Pub Date : 2024-01-19DOI: 10.32634/0869-8155-2024-378-1-60-64
E. V. Ivanov, A. V. Kapustin, N. N. Avduevskaya
The article presents the results of studies of the vaccine against infectious mastitis and acute postpartum endometritis of cows inactivated. The specific efficacy of the vaccine was determined by the number of cases of cows with endometritis. The disease was recorded in 14.2% of the experimental and 52.5% of the animals in the control group within a month after calving.An experiment was conducted comparing the effectiveness of two immunization regimens, the first of which provides for primary vaccination of pregnant animals, the second — the beginning of immunization of heifers from 6–8 months of age, followed by a single injection every 6 months at a dose of 3 ml. During the experiment, endometritis was detected in 10% of cows in the 1st and 2nd experimental groups, which is 3.7 times lower than in animals in the control group (36.6%). Thus, it was established that both animal immunization schemes are effective in combating the incidence of calving cows with endometritis.When studying the intrauterine secretions of cows with endometritis, a significant effect of immunization on the species composition of pathogens was established. Thus, in cows of the control group, E.coli with endometritis was isolated 3.4 times more often than in animals of the experimental group. A similar pattern was observed for other microorganisms: the number of S. aureus isolates detected was 2.2 times less in the experimental group, S. agalactiae — 15.0 times, S. dysgalactiae — 2.0 times, S. uberis — 6.0 times, S. pyogenes — 3.0 times, K. pneumoniae — 3.4 times less in comparison with the control group.
{"title":"The effectiveness of vaccination in acute postpartum endometritis of cows","authors":"E. V. Ivanov, A. V. Kapustin, N. N. Avduevskaya","doi":"10.32634/0869-8155-2024-378-1-60-64","DOIUrl":"https://doi.org/10.32634/0869-8155-2024-378-1-60-64","url":null,"abstract":"The article presents the results of studies of the vaccine against infectious mastitis and acute postpartum endometritis of cows inactivated. The specific efficacy of the vaccine was determined by the number of cases of cows with endometritis. The disease was recorded in 14.2% of the experimental and 52.5% of the animals in the control group within a month after calving.An experiment was conducted comparing the effectiveness of two immunization regimens, the first of which provides for primary vaccination of pregnant animals, the second — the beginning of immunization of heifers from 6–8 months of age, followed by a single injection every 6 months at a dose of 3 ml. During the experiment, endometritis was detected in 10% of cows in the 1st and 2nd experimental groups, which is 3.7 times lower than in animals in the control group (36.6%). Thus, it was established that both animal immunization schemes are effective in combating the incidence of calving cows with endometritis.When studying the intrauterine secretions of cows with endometritis, a significant effect of immunization on the species composition of pathogens was established. Thus, in cows of the control group, E.coli with endometritis was isolated 3.4 times more often than in animals of the experimental group. A similar pattern was observed for other microorganisms: the number of S. aureus isolates detected was 2.2 times less in the experimental group, S. agalactiae — 15.0 times, S. dysgalactiae — 2.0 times, S. uberis — 6.0 times, S. pyogenes — 3.0 times, K. pneumoniae — 3.4 times less in comparison with the control group.","PeriodicalId":7465,"journal":{"name":"Agrarian science","volume":"04 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139612679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-19DOI: 10.32634/0869-8155-2024-378-1-52-59
A. D. Lemyakin, K. Sabetova, A. A. Chaitsky, P. O. Shchegolev, L. S. Badanina
Relevance. The genotype of the animal plays a significant role in realizing its productive potential, along with other factors affecting dairy productivity. Marker-oriented breeding (MAS) of cattle, which utilizes genetic markers associated with economically valuable traits, has gained popularity in the last two decades. One such marker is the thyroglobulin (TG) gene, which is involved in the production of a thyroid prohormone.Methods.The research was conducted on cows of Kostroma and black-and-white breeds at breeding farms in the Kostroma region. Genetic material was obtained from the tail vein of the cows using individual vacuum systems with an anticoagulant. The polymorphism of the TG gene (rs135751032) was determined using RT-PCR and detection by hybridization-fluorescence method. The results were processed using biometric analysis and calculation of population-genetic indicators.Results. In the populations of cows of the Kostroma and black-and-white breeds of the Kostroma region, the predominance of carriers of the CC genotype and the C allele of the thyroglobulin gene was established. At the same time, among cows of these breeds, there is a tendency to higher milk productivity in heterozygous animals. The nature of the associative relationship between TG genotypes and milk productivity indicators differed significantly for each individual herd. This indicates the need to study TG in combination with other DNA markers of milk productivity and metabolism. Statistically significant differences between cows of different TG genotypes were found only in full-aged animals, which perhaps indicates the prospects of using TG to determine the genetic potential of productive longevity of cows.
相关性。动物的基因型与影响奶牛生产率的其他因素一样，在发挥其生产潜力方面起着重要作用。以标记为导向的牛育种（MAS）利用与具有经济价值的性状相关的遗传标记，在过去二十年中得到了普及。其中一个标记是甲状腺球蛋白（TG）基因，该基因参与甲状腺原激素的生产。使用带有抗凝剂的独立真空系统从奶牛尾静脉获取遗传物质。采用 RT-PCR 和杂交荧光法检测 TG 基因（rs135751032）的多态性。结果采用生物计量分析和种群遗传指标计算方法进行处理。在科斯特罗马地区的科斯特罗马和黑白品种奶牛群体中，甲状腺球蛋白基因的 CC 基因型和 C 等位基因携带者占多数。同时，在这些品种的奶牛中，杂合子动物的产奶量呈上升趋势。在每个牛群中，甲状腺球蛋白基因型与产奶量指标之间的关联关系的性质差异很大。这表明有必要将 TG 与其他有关牛奶生产率和新陈代谢的 DNA 标记结合起来研究。不同 TG 基因型的奶牛之间仅在全龄动物中发现了统计学意义上的显著差异，这或许表明了利用 TG 确定奶牛生产寿命遗传潜力的前景。
{"title":"Research of thyroglobulin gene polymorphism in cows of Kostroma and black-and-white breeds of Kostroma region","authors":"A. D. Lemyakin, K. Sabetova, A. A. Chaitsky, P. O. Shchegolev, L. S. Badanina","doi":"10.32634/0869-8155-2024-378-1-52-59","DOIUrl":"https://doi.org/10.32634/0869-8155-2024-378-1-52-59","url":null,"abstract":"Relevance. The genotype of the animal plays a significant role in realizing its productive potential, along with other factors affecting dairy productivity. Marker-oriented breeding (MAS) of cattle, which utilizes genetic markers associated with economically valuable traits, has gained popularity in the last two decades. One such marker is the thyroglobulin (TG) gene, which is involved in the production of a thyroid prohormone.Methods.The research was conducted on cows of Kostroma and black-and-white breeds at breeding farms in the Kostroma region. Genetic material was obtained from the tail vein of the cows using individual vacuum systems with an anticoagulant. The polymorphism of the TG gene (rs135751032) was determined using RT-PCR and detection by hybridization-fluorescence method. The results were processed using biometric analysis and calculation of population-genetic indicators.Results. In the populations of cows of the Kostroma and black-and-white breeds of the Kostroma region, the predominance of carriers of the CC genotype and the C allele of the thyroglobulin gene was established. At the same time, among cows of these breeds, there is a tendency to higher milk productivity in heterozygous animals. The nature of the associative relationship between TG genotypes and milk productivity indicators differed significantly for each individual herd. This indicates the need to study TG in combination with other DNA markers of milk productivity and metabolism. Statistically significant differences between cows of different TG genotypes were found only in full-aged animals, which perhaps indicates the prospects of using TG to determine the genetic potential of productive longevity of cows.","PeriodicalId":7465,"journal":{"name":"Agrarian science","volume":"88 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139612946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-19DOI: 10.32634/0869-8155-2024-378-1-81-85
O. Voronina, N. Kolesnik, A. Savina, R. Rykov, S. Y. Zaitsev
Relevance. Goat’s milk has great potential for creating functional food products, including those with high antioxidant activity, which increases their therapeutic and preventive significance. A mathematical model has been proposed to evaluate the antioxidant activity of goat’s milk.Methods. The biochemical parameters of goat’s milk were analyzed using the MilkoScan 7 / Fossomatic 7 DC system (Denmark). The analysis of the total amount of water-soluble antioxidants was performed on the device “Tsvetyauza 01-AA”. Statistical processing of the results was carried out in the Microsoft Excel program using the Data Analysis package, in the R program (“Psych” package). The reliability of the differences was assessed using the Mann — Whitney U-test. Milk samples were obtained from Alpine goats in the summer of 2022 and 2023.Results. The antioxidant activity of goat›s milk was established at the level of 21.99 ± 1.48 mg/g (2022) and 22.30 ± 1.91 mg/g (2023). The best obtained regression equation model is characterized by a high multiple correlation coefficient (0.947) and is significant by the F-criterion (0.01). In the proposed model, for a preliminary assessment of the antioxidant activity of goat›s milk, it is necessary to use only one parameter of biochemical analysis — caseins. This model allows for a preliminary assessment of the antioxidant activity of goat milk. There are no significant differences between the data obtained in the experiment and the data obtained using the regression equation according to the t-criterion.
相关性。山羊奶在制造功能性食品方面具有巨大潜力，包括那些具有高抗氧化活性的产品，这增加了它们的治疗和预防意义。我们提出了一个数学模型来评估山羊奶的抗氧化活性。使用 MilkoScan 7 / Fossomatic 7 DC 系统（丹麦）分析了羊奶的生化参数。水溶性抗氧化剂总量的分析是在 "Tsvetyauza 01-AA "设备上进行的。结果的统计处理在 Microsoft Excel 程序的数据分析软件包和 R 程序（"Psych "软件包）中进行。采用曼-惠特尼 U 检验法评估差异的可靠性。牛奶样本取自 2022 年和 2023 年夏季的阿尔卑斯山山羊。山羊奶的抗氧化活性分别为 21.99 ± 1.48 mg/g（2022 年）和 22.30 ± 1.91 mg/g（2023 年）。所得到的最佳回归方程模型具有较高的多重相关系数（0.947），并且在 F 标准（0.01）下具有显著性。在建议的模型中，为了初步评估山羊奶的抗氧化活性，只需使用一个生化分析参数--酪蛋白。该模型可对羊奶的抗氧化活性进行初步评估。根据 t 标准，实验得出的数据与使用回归方程得出的数据之间没有明显差异。
{"title":"Antioxidant activity of goat›s milk with variants of multiple regression model","authors":"O. Voronina, N. Kolesnik, A. Savina, R. Rykov, S. Y. Zaitsev","doi":"10.32634/0869-8155-2024-378-1-81-85","DOIUrl":"https://doi.org/10.32634/0869-8155-2024-378-1-81-85","url":null,"abstract":"Relevance. Goat’s milk has great potential for creating functional food products, including those with high antioxidant activity, which increases their therapeutic and preventive significance. A mathematical model has been proposed to evaluate the antioxidant activity of goat’s milk.Methods. The biochemical parameters of goat’s milk were analyzed using the MilkoScan 7 / Fossomatic 7 DC system (Denmark). The analysis of the total amount of water-soluble antioxidants was performed on the device “Tsvetyauza 01-AA”. Statistical processing of the results was carried out in the Microsoft Excel program using the Data Analysis package, in the R program (“Psych” package). The reliability of the differences was assessed using the Mann — Whitney U-test. Milk samples were obtained from Alpine goats in the summer of 2022 and 2023.Results. The antioxidant activity of goat›s milk was established at the level of 21.99 ± 1.48 mg/g (2022) and 22.30 ± 1.91 mg/g (2023). The best obtained regression equation model is characterized by a high multiple correlation coefficient (0.947) and is significant by the F-criterion (0.01). In the proposed model, for a preliminary assessment of the antioxidant activity of goat›s milk, it is necessary to use only one parameter of biochemical analysis — caseins. This model allows for a preliminary assessment of the antioxidant activity of goat milk. There are no significant differences between the data obtained in the experiment and the data obtained using the regression equation according to the t-criterion.","PeriodicalId":7465,"journal":{"name":"Agrarian science","volume":"87 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139612792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-19DOI: 10.32634/0869-8155-2024-378-1-40-51
B. V. Violin, M. Rebezov
To analyze the current state of the journal “Agrarian Science”, the prospects for its development and correction of editorial policy, some indicators of the journal›s publication activity for 2023 are presented. In 2023, 248 scientific articles were published in 12 issues of the journal “Agrarian Science”in four sections presented in the journal — Agronomy; Animal Science and Veterinary Medicine; Agroengineering and food technologies; Economics, which is an average of 20.7 articles per issue. The editorial board provides open and free access to all materials published in the issues of the magazine. The average number of authors in a scientific article of the journal “Agrarian Science” is 3.2 people. In 2023, the number of publications with the participation of foreign authors amounted to 15 articles (6% of the total number), and Kazakhstan is the most active foreign country among those published in the journal in 2023 — 73% of the total number of publications among foreign authors. The average number of sources in the bibliographic lists of scientific articles of the journal for 2023 is 17.2 references. The main authors of the articles are candidates of sciences (or PhD) — 47.1% of the total number of authors. The number of publications funded from various scientific foundations or grants is 119, or 49% of the total number.
{"title":"Analysis of publication activity of the journal “Agrarian Science” for 2023","authors":"B. V. Violin, M. Rebezov","doi":"10.32634/0869-8155-2024-378-1-40-51","DOIUrl":"https://doi.org/10.32634/0869-8155-2024-378-1-40-51","url":null,"abstract":"To analyze the current state of the journal “Agrarian Science”, the prospects for its development and correction of editorial policy, some indicators of the journal›s publication activity for 2023 are presented. In 2023, 248 scientific articles were published in 12 issues of the journal “Agrarian Science”in four sections presented in the journal — Agronomy; Animal Science and Veterinary Medicine; Agroengineering and food technologies; Economics, which is an average of 20.7 articles per issue. The editorial board provides open and free access to all materials published in the issues of the magazine. The average number of authors in a scientific article of the journal “Agrarian Science” is 3.2 people. In 2023, the number of publications with the participation of foreign authors amounted to 15 articles (6% of the total number), and Kazakhstan is the most active foreign country among those published in the journal in 2023 — 73% of the total number of publications among foreign authors. The average number of sources in the bibliographic lists of scientific articles of the journal for 2023 is 17.2 references. The main authors of the articles are candidates of sciences (or PhD) — 47.1% of the total number of authors. The number of publications funded from various scientific foundations or grants is 119, or 49% of the total number.","PeriodicalId":7465,"journal":{"name":"Agrarian science","volume":"34 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139611877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-20DOI: 10.32634/0869-8155-2023-377-12-138-142
Z. Muradyan, A. I. Albulov, R. Rogov
Relevance. Annotation. Experimental data on improving the production technology of regulated enzymatic cleavage of chitosan to obtain its low-molecular derivatives are presented. Experimental studies were carried out in the conditions of the Department of Disease Diagnostics, Therapy, Obstetrics and Animal Reproduction of the Moscow State Academy of Veterinary Medicine and Biotechnology — MVA named after K.I. Skryabin and on the basis of the All-Russian Research and Technological Institute of the Biological Industry.Methods. Acid-soluble chitosan from king crab shell (MM 700 kDa, SDA 85%), chitosan succinate (MM 330 kDa, SZ 75.2%), low-molecular food chitosan (hydrochloride) (MM 50 kDa) were used as raw materials for obtaining new modifications of chitosan, gel chitosan (2% solution in 2% acetic acid) manufactured by Bioprogress LLC.Results. As a result of the experiment, the technological parameters of obtaining low-molecular chitosan by enzymatic hydrolysis were selected. The greatest decrease in the dynamic viscosity of chitosan was reliably observed in the pilot-industrial series II (by 29.3 times), and the pilot-industrial series I and III — by 6.9 and 10.6 times, respectively. As a result of enzymatic hydrolysis of chitosan in the pilot series II, it was possible to reduce the molecular weight from 700 to 24 kDa, and in series I and III — to 102 and 66 kDa. Based on the research results, a technological scheme for obtaining low-molecular-weight chitosan derivatives by enzymatic hydrolysis has been developed, tested and proposed for industrial use, technical conditions have been developed.
相关性。注释。本文介绍了改进壳聚糖酶解生产技术以获得其低分子衍生物的实验数据。实验研究是在以 K.I. Skryabin 命名的莫斯科国立兽医和生物技术学院（MVA）疾病诊断、治疗、产科和动物繁殖系以及全俄生物工业研究和技术研究所的条件下进行的。从帝王蟹壳中提取的酸溶性壳聚糖（MM 700 kDa，SDA 85%）、琥珀酸壳聚糖（MM 330 kDa，SZ 75.2%）、低分子食品壳聚糖（盐酸盐）（MM 50 kDa）被用作获得壳聚糖新改性的原料，即生物进步有限责任公司生产的凝胶壳聚糖（2%醋酸中的2%溶液）。实验结果选择了用酶水解法获得低分子壳聚糖的技术参数。在中试工业系列 II 中观察到壳聚糖动态粘度的最大降幅（29.3 倍），在中试工业系列 I 和 III 中观察到壳聚糖动态粘度的最大降幅（分别为 6.9 倍和 10.6 倍）。在中试系列 II 中，通过酶水解壳聚糖，可以将分子量从 700 kDa 降至 24 kDa，而在系列 I 和 III 中，则分别降至 102 kDa 和 66 kDa。在研究成果的基础上，开发了一种通过酶水解获得低分子量壳聚糖衍生物的技术方案，并对其进行了试验，提出了用于工业的技术条件。
{"title":"Improving the technology for obtaining of low molecular weight chitosan under conditions of controlled enzymatic cleavage","authors":"Z. Muradyan, A. I. Albulov, R. Rogov","doi":"10.32634/0869-8155-2023-377-12-138-142","DOIUrl":"https://doi.org/10.32634/0869-8155-2023-377-12-138-142","url":null,"abstract":"Relevance. Annotation. Experimental data on improving the production technology of regulated enzymatic cleavage of chitosan to obtain its low-molecular derivatives are presented. Experimental studies were carried out in the conditions of the Department of Disease Diagnostics, Therapy, Obstetrics and Animal Reproduction of the Moscow State Academy of Veterinary Medicine and Biotechnology — MVA named after K.I. Skryabin and on the basis of the All-Russian Research and Technological Institute of the Biological Industry.Methods. Acid-soluble chitosan from king crab shell (MM 700 kDa, SDA 85%), chitosan succinate (MM 330 kDa, SZ 75.2%), low-molecular food chitosan (hydrochloride) (MM 50 kDa) were used as raw materials for obtaining new modifications of chitosan, gel chitosan (2% solution in 2% acetic acid) manufactured by Bioprogress LLC.Results. As a result of the experiment, the technological parameters of obtaining low-molecular chitosan by enzymatic hydrolysis were selected. The greatest decrease in the dynamic viscosity of chitosan was reliably observed in the pilot-industrial series II (by 29.3 times), and the pilot-industrial series I and III — by 6.9 and 10.6 times, respectively. As a result of enzymatic hydrolysis of chitosan in the pilot series II, it was possible to reduce the molecular weight from 700 to 24 kDa, and in series I and III — to 102 and 66 kDa. Based on the research results, a technological scheme for obtaining low-molecular-weight chitosan derivatives by enzymatic hydrolysis has been developed, tested and proposed for industrial use, technical conditions have been developed.","PeriodicalId":7465,"journal":{"name":"Agrarian science","volume":"117 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138958470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-20DOI: 10.32634/0869-8155-2023-377-12-102-107
V. Bychkova, O. Kibalnik, I. A. Sazonova, O. Kameneva
Relevance. Currently, the study of the interaction of these traits with each other is a very important task of breeding. In the work, 15 varieties of grain sorghum were studied, differing in morphological parameters, growing season, degree of grain ripening, biochemical composition and yield of grain and green mass. The purpose of the study is to study the interaction of environmental conditions on the yield and biochemical composition of sorghum grain and to assess the significance of these relationships.Methods. The studies were conducted in 2020, 2021 and 2022. The years of research differed in meteorological conditions (hydrothermal Selyaninov coefficient (SCC) was 0.56–0.76). To assess the biochemical composition in sorghum grain, the spectroscopy method was used using an infrared analyzer Spectra Star XT. The grain yield was determined according to the generally accepted method. Results. The experimental results were processed using the “Agros 2.09” software package by the method of correlation analysis. Based on the results of three years of research, a correlation was established between biochemical composition and the meteorological conditions of the year (sum of temperatures, total precipitation, SCC), as well as a significant effect of these traits on the yield of sorghum grain.
{"title":"Influence of meteorological conditions of the year on the biochemical composition of sorghum grain","authors":"V. Bychkova, O. Kibalnik, I. A. Sazonova, O. Kameneva","doi":"10.32634/0869-8155-2023-377-12-102-107","DOIUrl":"https://doi.org/10.32634/0869-8155-2023-377-12-102-107","url":null,"abstract":"Relevance. Currently, the study of the interaction of these traits with each other is a very important task of breeding. In the work, 15 varieties of grain sorghum were studied, differing in morphological parameters, growing season, degree of grain ripening, biochemical composition and yield of grain and green mass. The purpose of the study is to study the interaction of environmental conditions on the yield and biochemical composition of sorghum grain and to assess the significance of these relationships.Methods. The studies were conducted in 2020, 2021 and 2022. The years of research differed in meteorological conditions (hydrothermal Selyaninov coefficient (SCC) was 0.56–0.76). To assess the biochemical composition in sorghum grain, the spectroscopy method was used using an infrared analyzer Spectra Star XT. The grain yield was determined according to the generally accepted method. Results. The experimental results were processed using the “Agros 2.09” software package by the method of correlation analysis. Based on the results of three years of research, a correlation was established between biochemical composition and the meteorological conditions of the year (sum of temperatures, total precipitation, SCC), as well as a significant effect of these traits on the yield of sorghum grain.","PeriodicalId":7465,"journal":{"name":"Agrarian science","volume":"123 27","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138953718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-20DOI: 10.32634/0869-8155-2023-377-12-118-123
V. Ostroshenko, M. N. Shein, L. Ostroshenko
Relevance. Currently, one of the main tasks in forestry is to obtain high–quality planting material. Studies are being conducted on accelerated cultivation of coniferous and deciduous plants in forest nurseries with the use of growth stimulants that positively affect seed germination, the growth of biometric indicators, the safety and survival of plants. In this regard, the use of plant growth stimulants, which also include “Zircon” and “Ferovit”, is relevant.Methods. The effect of root feeding with Ferovit and Zircon preparations on morphometric parameters of three-year-old Korean cedar seedlings (Pinus koraiensis Siebold et Zucc.) was studied. Venue: nursery of the Mountain Taiga Station — branch of the Federal Research Center for Biodiversity of Terrestrial Biota of East Asia FEB RAS in 2019—2022. Parameters such as the height of seedlings, height gain, root neck diameter, root length and biomass were studied.Results. It was found that a significant effect is provided by a variant of root fertilization of seedlings with the use of “Zircon” and “Ferovit” at a concentration of 1.5 ml / 3 l of water solution. The indicators of the height of seedlings compared to the control increased by 126.1%, height increase — by 136.7%, root neck diameter — by 20%, root length — by 21.8%, biomass — by 252.1%.
{"title":"The prolonged influence of stimulants on the growth of three-year-old Korean pine (Pinus koraiensis Siebold et Zucc.) seedlings","authors":"V. Ostroshenko, M. N. Shein, L. Ostroshenko","doi":"10.32634/0869-8155-2023-377-12-118-123","DOIUrl":"https://doi.org/10.32634/0869-8155-2023-377-12-118-123","url":null,"abstract":"Relevance. Currently, one of the main tasks in forestry is to obtain high–quality planting material. Studies are being conducted on accelerated cultivation of coniferous and deciduous plants in forest nurseries with the use of growth stimulants that positively affect seed germination, the growth of biometric indicators, the safety and survival of plants. In this regard, the use of plant growth stimulants, which also include “Zircon” and “Ferovit”, is relevant.Methods. The effect of root feeding with Ferovit and Zircon preparations on morphometric parameters of three-year-old Korean cedar seedlings (Pinus koraiensis Siebold et Zucc.) was studied. Venue: nursery of the Mountain Taiga Station — branch of the Federal Research Center for Biodiversity of Terrestrial Biota of East Asia FEB RAS in 2019—2022. Parameters such as the height of seedlings, height gain, root neck diameter, root length and biomass were studied.Results. It was found that a significant effect is provided by a variant of root fertilization of seedlings with the use of “Zircon” and “Ferovit” at a concentration of 1.5 ml / 3 l of water solution. The indicators of the height of seedlings compared to the control increased by 126.1%, height increase — by 136.7%, root neck diameter — by 20%, root length — by 21.8%, biomass — by 252.1%.","PeriodicalId":7465,"journal":{"name":"Agrarian science","volume":"118 37","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138953897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-20DOI: 10.32634/0869-8155-2023-377-12-114-117
N. I. Kirillova, I. Degtyareva
Relevance. The study of test-plants potential consists in determining indicators that reflect the quality of the seed material and its required quantity during production.Methods. The objects of research are modified biofungicides created on the basis of the microscopic fungus Trichoderma viride and natural minerals: diatomite, glauconite, sapropel, zeolite.The evaluation of the characteristics of the test-plant (spring wheat variety Ulyanovsk 105) when treated with modified biofungicides was carried out on the basis of indicators as germination energy and seed germination, biometric data (seedling and root length) and biomass (seedling and root weight).Results. It has been established that seed treatment with the created biofungicides has a positive effect on the germination energy (82.0–96.0%) and germination (87.0–96.0%) of the test plant under study. In all experimental variants, the length of the seedling was on average 11.0% higher compared to the control. The maximum values of this indicator were noted in the variants when the seeds of spring wheat were treated with a biofungicide based on T. viride (by 18.59%), as well as in the complex treatment of T. viride with a zeolite fraction of 0.04 mm (by 21.70%) and with diatomite (by 12.28%). Stimulation of the root system development by 8.71–21.13% was also noted. In all experimental variants, seed treatment with new biofungicides stimulates the formation of test plant biomass. The best values were noted in the variants with the treatment of T. viride and T. viride with zeolite (fraction 0.04 mm), where the weight gain of the seedling to the control is 25.67% and 20.86%, and the root weight is 25.23% and 18.39%, respectively. The use of modified biofungicides based on micromycete T. viride and natural minerals is promising for obtaining environmentally friendly crop products.
{"title":"Test-plant characteristics using modified biofungicides treatment","authors":"N. I. Kirillova, I. Degtyareva","doi":"10.32634/0869-8155-2023-377-12-114-117","DOIUrl":"https://doi.org/10.32634/0869-8155-2023-377-12-114-117","url":null,"abstract":"Relevance. The study of test-plants potential consists in determining indicators that reflect the quality of the seed material and its required quantity during production.Methods. The objects of research are modified biofungicides created on the basis of the microscopic fungus Trichoderma viride and natural minerals: diatomite, glauconite, sapropel, zeolite.The evaluation of the characteristics of the test-plant (spring wheat variety Ulyanovsk 105) when treated with modified biofungicides was carried out on the basis of indicators as germination energy and seed germination, biometric data (seedling and root length) and biomass (seedling and root weight).Results. It has been established that seed treatment with the created biofungicides has a positive effect on the germination energy (82.0–96.0%) and germination (87.0–96.0%) of the test plant under study. In all experimental variants, the length of the seedling was on average 11.0% higher compared to the control. The maximum values of this indicator were noted in the variants when the seeds of spring wheat were treated with a biofungicide based on T. viride (by 18.59%), as well as in the complex treatment of T. viride with a zeolite fraction of 0.04 mm (by 21.70%) and with diatomite (by 12.28%). Stimulation of the root system development by 8.71–21.13% was also noted. In all experimental variants, seed treatment with new biofungicides stimulates the formation of test plant biomass. The best values were noted in the variants with the treatment of T. viride and T. viride with zeolite (fraction 0.04 mm), where the weight gain of the seedling to the control is 25.67% and 20.86%, and the root weight is 25.23% and 18.39%, respectively. The use of modified biofungicides based on micromycete T. viride and natural minerals is promising for obtaining environmentally friendly crop products.","PeriodicalId":7465,"journal":{"name":"Agrarian science","volume":"10 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139168749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-20DOI: 10.32634/0869-8155-2023-377-12-108-113
A. Ashiev, K. N. Khabibullin, M. Skulova
Relevance. An integral part in the creation of new varieties is the study of the elements of the crop structure of collection samples, and the determination of their impact on yield will allow the inclusion of samples with their optimal ratio in the breeding process.Methods. The research was carried out in the fields of the “Donskoy” Research Center in 2019–2021 in accordance with the Methodology of the State Variety Testing (2019) and the Methodology of Field Experience (2012). The objects of research are 85 soybean samples from the world collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIGRR) of domestic and foreign breeding.Results. The most productive were samples with plant height 55–70 cm, attachment height of the lower bean 16–17 cm and 20–21 cm, number of branches 1.0–1.5 pcs/rast, number of beans 30–35 pcs/rast, number of seeds 65–70 pcs/rast, weight of 1000 seeds 140–150 g and 190–200 g, weight of seeds from a plant 11–12 g, period vegetation period is 112–114 days, protein content is 39.0–39.5%, fat content is 21.0–21.5%.
{"title":"The influence of crop structure elements on the seed productivity of soybean collection samples","authors":"A. Ashiev, K. N. Khabibullin, M. Skulova","doi":"10.32634/0869-8155-2023-377-12-108-113","DOIUrl":"https://doi.org/10.32634/0869-8155-2023-377-12-108-113","url":null,"abstract":"Relevance. An integral part in the creation of new varieties is the study of the elements of the crop structure of collection samples, and the determination of their impact on yield will allow the inclusion of samples with their optimal ratio in the breeding process.Methods. The research was carried out in the fields of the “Donskoy” Research Center in 2019–2021 in accordance with the Methodology of the State Variety Testing (2019) and the Methodology of Field Experience (2012). The objects of research are 85 soybean samples from the world collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIGRR) of domestic and foreign breeding.Results. The most productive were samples with plant height 55–70 cm, attachment height of the lower bean 16–17 cm and 20–21 cm, number of branches 1.0–1.5 pcs/rast, number of beans 30–35 pcs/rast, number of seeds 65–70 pcs/rast, weight of 1000 seeds 140–150 g and 190–200 g, weight of seeds from a plant 11–12 g, period vegetation period is 112–114 days, protein content is 39.0–39.5%, fat content is 21.0–21.5%.","PeriodicalId":7465,"journal":{"name":"Agrarian science","volume":"17 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138956500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-20DOI: 10.32634/0869-8155-2023-377-12-58-66
P. Burkov, M. Derkho, M. Rebezov, P. N. Shcherbakov, A. O. Derkho, K. Stepanova
Relevance. Reproductive insufficiency of sows associated with circovirus infection is widespread in pig breeding enterprises. Therefore, an increase in the effectiveness of immunity formed in animals during the post-vaccination period is of crucial importance in the formation of the reproductive potential of sows.Methods. The work was performed on sows, which at the end of the suckling period during weaning of piglets (on the 21st day after delivery) were vaccinated against CVS-2 with the “Ingelvak Circoflex” vaccine (Germany). In the experimental group, vaccination was combined with a two-time administration of a targeted immunobiostimulator “Transfer Factor” at a dose of 5 ml per head with an interval of 7 days (the second administration of “Transfer Factor” was carried out during vaccination). The effectiveness of post-vaccination immunity was evaluated based on the results of immunological, zootechnical and statistical studies.Results. It was found that the formation of post-vaccination immunity in control group sows occurs under conditions of an increase in the concentration of immunoglobulins in the blood (∑G + M + A) by 1.25–1.59 times due to IgG and IdM, a decrease in the absolute number of T- and B-lymphocytes by 11.84–15.51% and 14.12–17.51% while maintaining their percentage share in the total pool of lymphocytes, a decrease in the level of T-helpers by 34.27–36.47 against the background of an increase in cytotoxic lymphocytes and killers by 4.47–66.67%, which determines the yield of piglets per farrow in the amount of 12.5 heads and stillbirth at the level of 9.67%. The combination of vaccination with the introduction of a specific targeted immunobiostimulator in sows of the experimental group determines in the post–vaccination period; an increase in the concentration of immunoglobulins in the blood by 24.93–71.56% due to IgG; a decrease in the number of T-lymphocytes by 19.50–23.76% against the background of an increase in B-lymphocytes by 20.00–21.25%; preservation of the absolute number of T-helpers and reducing the number of cytotoxic lymphocytes and killers by 2.14–3.00 times, contributing to a 3.24% reduction in stillbirth and an increase in the yield of piglets per farrowing to 13.0 heads.
{"title":"Immunological status of sows during the reproductive cycle and correction of its condition with an antigen-directed biostimulator","authors":"P. Burkov, M. Derkho, M. Rebezov, P. N. Shcherbakov, A. O. Derkho, K. Stepanova","doi":"10.32634/0869-8155-2023-377-12-58-66","DOIUrl":"https://doi.org/10.32634/0869-8155-2023-377-12-58-66","url":null,"abstract":"Relevance. Reproductive insufficiency of sows associated with circovirus infection is widespread in pig breeding enterprises. Therefore, an increase in the effectiveness of immunity formed in animals during the post-vaccination period is of crucial importance in the formation of the reproductive potential of sows.Methods. The work was performed on sows, which at the end of the suckling period during weaning of piglets (on the 21st day after delivery) were vaccinated against CVS-2 with the “Ingelvak Circoflex” vaccine (Germany). In the experimental group, vaccination was combined with a two-time administration of a targeted immunobiostimulator “Transfer Factor” at a dose of 5 ml per head with an interval of 7 days (the second administration of “Transfer Factor” was carried out during vaccination). The effectiveness of post-vaccination immunity was evaluated based on the results of immunological, zootechnical and statistical studies.Results. It was found that the formation of post-vaccination immunity in control group sows occurs under conditions of an increase in the concentration of immunoglobulins in the blood (∑G + M + A) by 1.25–1.59 times due to IgG and IdM, a decrease in the absolute number of T- and B-lymphocytes by 11.84–15.51% and 14.12–17.51% while maintaining their percentage share in the total pool of lymphocytes, a decrease in the level of T-helpers by 34.27–36.47 against the background of an increase in cytotoxic lymphocytes and killers by 4.47–66.67%, which determines the yield of piglets per farrow in the amount of 12.5 heads and stillbirth at the level of 9.67%. The combination of vaccination with the introduction of a specific targeted immunobiostimulator in sows of the experimental group determines in the post–vaccination period; an increase in the concentration of immunoglobulins in the blood by 24.93–71.56% due to IgG; a decrease in the number of T-lymphocytes by 19.50–23.76% against the background of an increase in B-lymphocytes by 20.00–21.25%; preservation of the absolute number of T-helpers and reducing the number of cytotoxic lymphocytes and killers by 2.14–3.00 times, contributing to a 3.24% reduction in stillbirth and an increase in the yield of piglets per farrowing to 13.0 heads. ","PeriodicalId":7465,"journal":{"name":"Agrarian science","volume":"12 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138954772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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