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Rice breeding for multi‐canopy system: Estimations of genetic parameters and response to selection 多冠系统水稻育种:遗传参数估计和对选择的响应
IF 2.1 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-06-30 DOI: 10.1002/agj2.21629
Ma'rifatus Sholehah, Willy Bayuardi Suwarno, Vany Putri Hapsari, Nisfia Nurfirdausy Sulistyo, Siti Marwiyah, Hajrial Aswidinnoor
One strategy currently being developed to increase rice (Oryza sativa L.) productivity is using a multi‐canopy cropping system in rice cultivation. This method involves planting tall and short rice genotypes in the same hill. The objective of this experiment was to estimate the genetic parameters and response to selection in multi‐canopy rice. Each experiment was arranged in an augmented randomized complete block design with five replications for the checks. In the first planting season, 200 F3 families from IPB196 and IPB197 populations were planted in monoculture and multi‐canopy as the short genotypes. IPB187‐F‐40‐1‐1 was used in multi‐canopy as the tall genotype. Selection of 25% based on grain weight per hill of short genotype in multi‐canopy was performed, and 50 families were selected and their F4 seeds were planted in the second season along with the same tall genotype. The results indicated the genotype × cropping system was significant for grain weight per hill in the F3 and F4 generations. Grain weight per hill has a similar realized h2ns in the multi‐canopy (0.58) with monoculture (0.54). Meanwhile, the response to selection in multi‐canopy (3.60) was higher compared to monoculture (2.09), and therefore the selection of rice lines for a multi‐canopy system should be conducted in the multi‐canopy environment. A selection percentage of 5% resulted in a higher response to selection. These findings may provide insight into the acceleration of breeding rice varieties for the multi‐canopy system.
目前正在开发的提高水稻(Oryza sativa L.)产量的一项战略是在水稻种植中采用多冠种植系统。这种方法是在同一山丘上种植高矮稻基因型。本实验的目的是估计多冠水稻的遗传参数和对选择的反应。每项实验均采用增强型随机完全区组设计,设五次重复。在第一个种植季节,200 个来自 IPB196 和 IPB197 群体的 F3 家系作为短基因型被种植在单一种植和多冠栽培中。IPB187-F-40-1-1 作为高基因型用于多冠种植。根据多冠矮基因型每丘粒重的 25% 进行筛选,选出 50 个家系,其 F4 种子与相同的高基因型一起在第二季种植。结果表明,在 F3 和 F4 代中,基因型×种植制度对每丘粒重的影响显著。多冠栽培(0.58)与单冠栽培(0.54)的每丘粒重具有相似的实现h2ns。同时,多冠系的选择反应(3.60)高于单冠系(2.09),因此多冠系水稻品系的选择应在多冠系环境中进行。选择比例为 5%时,对选择的反应较高。这些发现可为加速培育多冠系水稻品种提供启示。
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引用次数: 0
Representing cropping systems with the MEMS 2 ecosystem model 用 MEMS 2 生态系统模型表示种植系统
IF 2.1 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-06-30 DOI: 10.1002/agj2.21611
Yao Zhang, Alison E. King, Emma Hamilton, M. Francesca Cotrufo
Croplands have been the focus of substantial investigation due to their considerable potential for sequestering carbon. Understanding the potential for soil organic carbon (SOC) sequestration and necessary management strategies will be enabled with accurate process‐based models. Accurately representing crop growth and agricultural practices will be critical for realistic SOC modeling. The MEMS 2 model incorporates a current understanding of SOC formation and stabilization, measurable SOC pools, and deep SOC dynamics and is seen as a highly promising tool to inform management intervention for SOC sequestration. Thus far, MEMS 2 has been developed to represent grasslands. In this study, we further developed MEMS 2 to model annual grain crops and common agricultural practices, such as irrigation, fertilization, harvesting, and tillage. Using four Ameriflux sites, we demonstrated an accurate simulation of crop growth and development. Model performance was strong for simulating aboveground biomass (index of agreement [d] range of 0.89–0.98) and green leaf area index (d from 0.90 to 0.96) across corn, soybean, and winter wheat. Good agreement with observations was also achieved for net ecosystem CO2 exchange (d from 0.90 to 0.96), evapotranspiration (d from 0.91 to 0.94), and soil temperature (d of 0.96), while discrepancy with the available soil water content data remain (d from 0.14 to 0.81 at four depths to 100 cm). While we will continue model testing and improvement, MEMS 2 (version 2.14) has now demonstrated its ability to effectively simulate the growth of common grain crops and practices.
由于耕地具有相当大的固碳潜力,因此一直是大量调查的重点。有了基于过程的精确模型,就能了解土壤有机碳 (SOC) 固碳的潜力和必要的管理策略。准确反映作物生长和农业实践对于建立现实的 SOC 模型至关重要。MEMS 2 模型结合了当前对 SOC 形成和稳定、可测量的 SOC 池和深层 SOC 动态的理解,被视为一种非常有前途的工具,可为 SOC 固碳的管理干预提供信息。迄今为止,MEMS 2 已开发用于表示草原。在本研究中,我们进一步开发了 MEMS 2,以模拟一年生谷物作物和常见农业实践,如灌溉、施肥、收割和耕作。通过使用四个 Ameriflux 站点,我们展示了对作物生长和发育的精确模拟。在模拟玉米、大豆和冬小麦的地上生物量(一致指数[d]范围为 0.89-0.98)和绿叶面积指数(d 范围为 0.90 至 0.96)方面,模型表现出色。生态系统二氧化碳净交换量(d 从 0.90 到 0.96)、蒸散量(d 从 0.91 到 0.94)和土壤温度(d 为 0.96)也与观测结果保持良好一致,但与现有的土壤含水量数据仍存在差异(在 100 厘米以下的四个深度,d 从 0.14 到 0.81)。虽然我们将继续对模型进行测试和改进,但 MEMS 2(2.14 版)现已证明其有能力有效地模拟常见粮食作物的生长和实践。
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引用次数: 0
Developing statistical models of aflatoxin risk in peanuts using historical weather data 利用历史气象数据开发花生黄曲霉毒素风险统计模型
IF 2.1 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-06-30 DOI: 10.1002/agj2.21627
Da‐Young Kim, Fikadu Getachew, Barry L. Tillman, Brendan Zurweller, William M. Hammond, Alina Zare, Raegan Holton, Zachary Brym
Aflatoxin contamination in peanuts (Arachis hypogaea L.) is a significant public health risk. Aflatoxin is detected postharvest after inspection of loads associated with grading at peanut buying points, leaving growers and shellers in a precarious position. Stricter limits on aflatoxin contamination could restrict the United States access to international markets. Predicting aflatoxin risk remains challenging, but improved tools could help inform postharvest storage segregation decisions and alert industry stakeholders to seasonal threats. This study aimed to develop and evaluate multiple statistical models that estimate the regional status of peanut aflatoxin contamination based on preharvest weather conditions. Our approach expanded on an existing peanut aflatoxin model for which a new geographic area and time period were tested. Weather variables served as independent variables to predict the risk of aflatoxin as the proportion of samples with greater than 20 ppb and 4 ppb aflatoxin (PGT20 [the proportion of samples with greater than 20 ppb aflatoxin] and PGT4 [the proportion of samples with greater than 4 ppb aflatoxin], respectively) across 10 counties in Georgia for 2018–2022. Best‐performing models were developed through multiple linear stepwise regression explaining more than 72% and 41% of the variability in PGT20 and PGT4, respectively. Model performance further varied whether it was a year of low or high aflatoxin incidence, with temperature observed as a key influencing factor across best‐performing models. This study established an adaptive approach to monitoring and managing aflatoxin risk through statistical predictive modeling, with output targeting farmers, industry, regulators, and public health officials. Future model development will aim to improve interpretation and confidence with in‐season aflatoxin prediction and efficacy testing of this approach across space and time.
花生(Arachis hypogaea L.)中的黄曲霉毒素污染是一个重大的公共卫生风险。黄曲霉毒素是在收获后对与花生收购点分级相关的装载货物进行检查后检测出来的,这使种植者和剥壳者的处境岌岌可危。对黄曲霉毒素污染的更严格限制可能会限制美国进入国际市场。预测黄曲霉毒素风险仍然具有挑战性,但改进后的工具有助于为收获后贮藏隔离决策提供信息,并提醒行业利益相关者注意季节性威胁。这项研究旨在开发和评估多种统计模型,根据收获前的天气条件来估计花生黄曲霉毒素污染的区域状况。我们的方法扩展了现有的花生黄曲霉毒素模型,对新的地理区域和时间段进行了测试。天气变量作为自变量,用于预测佐治亚州 10 个县 2018-2022 年黄曲霉毒素含量超过 20 ppb 和 4 ppb 的样本比例(PGT20 [黄曲霉毒素含量超过 20 ppb 的样本比例] 和 PGT4 [黄曲霉毒素含量超过 4 ppb 的样本比例])。通过多元线性逐步回归建立的最佳模型分别解释了 PGT20 和 PGT4 变异的 72% 和 41% 以上。无论是黄曲霉毒素发生率较低的年份还是较高的年份,模型的表现都会有进一步的变化,在表现最佳的模型中,温度是一个关键的影响因素。这项研究通过统计预测建模建立了一种监测和管理黄曲霉毒素风险的适应性方法,其产出针对农民、工业、监管机构和公共卫生官员。未来的模型开发将致力于改进对季节性黄曲霉毒素预测的解释和信心,并对这一方法进行跨时空的有效性测试。
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引用次数: 0
Soybean planting dates and maturity groups: Maximizing yield potential and decreasing risk in Louisiana 大豆种植日期和成熟度组别:最大化路易斯安那州的产量潜力并降低风险
IF 2.1 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-06-30 DOI: 10.1002/agj2.21626
David Moseley, Andre Reis, Thanos Gentimis, Priscila Campos, Josh Copes, Melanie Netterville, Peters Egbedi, Dustin Harrell, Manoch Kongchum, Ronnie Levy, Boyd Padgett, Samuel Soignier, Derek Scroggs, Jason Sanders, Joe Pankey, Katarzyna Fic
Soybean [Glycine max (L.) Merr.] producers in Louisiana began shifting to an early soybean production system (ESPS) in the early 2000s by planting earlier maturing varieties in April and May. Although this shift in planting practices has been supported by research elsewhere beginning in the mid‐1990s, there is minimum data focusing on the ESPS across Louisiana. The overall objective of this research was to evaluate the effect of planting date, maturity group (MG), and their combination across a comprehensive set of yield environments in the state of Louisiana to determine the combination of optimum planting date and MG for soybean production. We gathered field data from the 2013 to 2020 seasons originated from three Louisiana State University AgCenter research stations and four seed companies. A total of 428, 926, and 331 observations were analyzed from the Northeast, Central, and Southwest Louisiana regions, respectively. When including all data from across Louisiana, the optimum planting date was April 30. Breaking by regions, the average optimum planting date for the Northeast Louisiana region was April 9. The Central and Southwest results were divided by MG section, and the approximate optimum planting dates were April 15 and May 15, respectively. These results support the ESPS for the Northeast and Central Louisiana regions, but not for the Southwest Louisiana region. Optimizing planting recommendation is a critical component for supporting the development of varieties suitable for the southern production systems of the United States.
路易斯安那州的大豆 [Glycine max (L.) Merr.] 生产者从 2000 年代初开始转向早熟大豆生产系统 (ESPS),在 4 月和 5 月种植早熟品种。尽管从 20 世纪 90 年代中期开始,这种种植方式的转变得到了其他地区研究的支持,但在整个路易斯安那州,有关 ESPS 的数据却很少。本研究的总体目标是评估种植日期、成熟度组(MG)及其组合在路易斯安那州一系列综合产量环境中的影响,以确定大豆生产的最佳种植日期和成熟度组组合。我们从路易斯安那州立大学农业中心的三个研究站和四家种子公司收集了 2013 年至 2020 年的田间数据。我们分别对路易斯安那州东北部、中部和西南部地区的 428、926 和 331 个观测数据进行了分析。如果将路易斯安那州各地的所有数据都包括在内,最佳播种日期为 4 月 30 日。按地区划分,路易斯安那州东北部地区的平均最佳播种日期为 4 月 9 日。中部和西南部的结果按 MG 分区划分,大致的最佳播种日期分别为 4 月 15 日和 5 月 15 日。这些结果支持路易斯安那州东北部和中部地区的 ESPS,但不支持路易斯安那州西南部地区的 ESPS。优化种植建议是支持开发适合美国南部生产系统的品种的关键要素。
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引用次数: 0
Reformulation of dicamba herbicide: Impacts on offsite transport and soybean damage 麦草畏除草剂的重新配制:对异地运输和大豆损害的影响
IF 2.1 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-06-29 DOI: 10.1002/agj2.21630
Caleb R. Hammer, Timothy J. Griffis, John M. Baker, Pamela J. Rice, Lara E. Frankson, Jeffrey L. Gunsolus, Matthew D. Erickson, Ke Xiao, Aarti P. Mistry, Debalin Sarangi
The herbicide dicamba (3,6‐dichloro‐2‐methoxybenzoic acid) is commonly used to control broadleaf weeds in soybeans. Dicamba, however, is susceptible to volatilization and drift, thereby causing significant plant damage to nontarget crops downwind. Dicamba was reformulated to reduce volatility and off‐target movement. The effectiveness of the dicamba reformulation was assessed by quantifying dicamba emissions following spray application and investigated how meteorological factors influenced the off‐target movement. The experiments were conducted at the University of Minnesota Agricultural Experiment Station (UMORE Park) during the growing season of 2018, 2019, 2021, and 2022. Multiple high‐flow polyurethane foam air samplers were used to measure dicamba concentrations downwind from a 4‐ha soybean field sprayed with dicamba. Dicamba emissions were estimated using backward Lagrangian modeling constrained by the air sample observations. The results indicate that dicamba emissions and downwind transport were significant for several days following application. Further, non‐traited soybeans located within 15–45 m showed substantial dicamba‐related damage. In warmer, drier seasons, increased dicamba emissions caused more severe damage to downwind soybeans, likely worsened by drought stress preventing recovery. Favorable atmospheric conditions that reduced potential drift can be difficult to achieve in terms of the typical weather experienced over agricultural sites in the Upper Midwest. These results indicate that the dicamba reformulation has not adequately prevented significant post‐spray volatilization losses and downwind transport.
除草剂麦草畏(3,6-二氯-2-甲氧基苯甲酸)常用于控制大豆中的阔叶杂草。然而,麦草畏容易挥发和漂移,从而对下风向的非目标作物造成严重的植物损害。对麦草畏进行了重新配制,以减少挥发性和非目标移动。通过量化麦草畏在喷洒后的排放量,评估了麦草畏改良配方的效果,并研究了气象因素对非目标移动的影响。实验于 2018 年、2019 年、2021 年和 2022 年的生长季节在明尼苏达大学农业试验站(UMORE Park)进行。使用多个高流量聚氨酯泡沫空气采样器测量喷洒了麦草畏的 4 公顷大豆田下风向的麦草畏浓度。在空气样本观测结果的约束下,使用后向拉格朗日模型估算麦草畏的排放量。结果表明,在喷洒麦草畏后的几天内,麦草畏的排放量和下风迁移量都很大。此外,位于 15-45 米范围内的无麦草畏大豆也受到了严重的麦草畏相关损害。在温暖干燥的季节,麦草畏排放量的增加会对下风向的大豆造成更严重的损害,而干旱压力可能会阻碍大豆的恢复。在上中西部农业区的典型天气条件下,很难实现减少潜在漂移的有利大气条件。这些结果表明,麦草畏改良配方并没有充分防止大量的喷洒后挥发损失和下风向迁移。
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引用次数: 0
Long‐term crop yield benefits of subsurface drainage on poorly drained soils 排水不良土壤的地下排水对作物产量的长期益处
IF 2.1 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-06-28 DOI: 10.1002/agj2.21621
Yichao Rui, Benjamin Goller, Eileen J. Kladivko
Subsurface drainage is an important agricultural practice that has been widely utilized in the US Midwest to improve the productivity of poorly drained soils. Although widely adopted, long‐term yield benefits of drainage, particularly with varying spacings, in an ever‐changing climate are largely unknown. The goals of this study were to assess how various drainage spacings (5, 10, and 20 m) impacted crop yields compared to the undrained control in a long‐term trial (started in 1984) in southeastern Indiana and how these effects were influenced by the amount of rainfall of specific periods of the growing season. Drainage treatments led to an increase in corn (Zea mays) yields (by 12%–17%) but did not significantly affect soybean (Glycine max) yields compared to the control. In the initial 10 years of the experiment, drainage benefits were subtle and corn yields did not vary significantly across spacing treatments, whereas in the most recent 10 corn years, the drainage treatment effects became more pronounced, likely due to the combined effects of long‐term drainage system and conservation practices of no‐till and cover crops. Over 37 years, corn yields remained stagnant in the undrained plots but progressively increased in the drained treatments. Both corn and soybean yields showed a negative correlation with rainfall 14 days post‐planting, while drainage spacing treatments partially mitigated this negative effect. Our findings underscore the importance of effective drainage as a necessary prerequisite for realizing the potential benefits of conservation practices and improved crop genetics for increased crop productivity.
地下排水是美国中西部地区广泛采用的一种重要农业措施,可提高排水不良土壤的生产力。尽管这种方法已被广泛采用,但在气候不断变化的情况下,排水(尤其是不同间距的排水)的长期产量效益在很大程度上仍是未知数。本研究的目的是评估在印第安纳州东南部的一项长期试验(始于 1984 年)中,与未排水对照相比,不同的排水间距(5 米、10 米和 20 米)对作物产量的影响,以及这些影响如何受到生长季节特定时期降雨量的影响。与对照组相比,排水处理使玉米(Zea mays)产量增加(12%-17%),但对大豆(Glycine max)产量没有显著影响。在实验的最初 10 年中,排水系统的效益并不明显,不同间距处理的玉米产量差异也不大,而在最近的 10 个玉米种植年中,排水系统处理的效果变得更加明显,这可能是由于长期排水系统以及免耕和覆盖作物等保护措施的共同作用。在 37 年的时间里,未排水地块的玉米产量一直停滞不前,但排水处理地块的玉米产量却逐渐增加。玉米和大豆的产量与播种后 14 天的降雨量呈负相关,而排水间距处理则部分缓解了这一负面影响。我们的研究结果强调了有效排水的重要性,它是实现保护措施的潜在效益和改良作物基因以提高作物产量的必要前提。
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引用次数: 0
Evaluating the effects of cotton intercropping on cool‐season perennial forage persistence, forage mass, and nutritive value in the southeastern United States 评估棉花间作对美国东南部冷季型多年生牧草持久性、牧草质量和营养价值的影响
IF 2.1 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-06-28 DOI: 10.1002/agj2.21625
Eric D. Billman, W. Tillman Myers
Integrated forage–row cropping systems provide important agronomic and economic benefits to producers. However, little attention has been given to incorporating forages into row crop systems unique to the southeastern United States. This study assessed the viability of intercropping cotton (Gossypium hirsutum L.) on perennial, cool‐season legumes during the summer months in the Southeast Coastal Plain over two production years. Treatments included a weedy fallow, annual ryegrass (ARG; Lolium multiflorum Lam.) monoculture, a red clover (RC; Trifolium pratense L.) and white clover (WC; Trifolium repens L.) mixture, and a three‐species mixture of ARG, RC, and WC. Plots were established in fall 2020 with forage grown until May 2021 and 2022, when plots were strip‐tilled and planted with cotton. Cotton was managed with minimal herbicide use to preserve perennial clovers. Results indicated WC was more persistent than RC (>40 plants m−2 vs. <40 plants m−2), and the presence of perennial clovers suppressed weeds at similar levels to the ARG monoculture (35 weeds m−2) during the second spring. Perennial clovers grew taller (4–5 cm) when mixed with ARG. The presence of clovers mixed with ARG during the second spring reduced acid detergent fiber (ADF) concentration and increased crude protein (CP) concentration (280 g ADF kg−1; 167 g CP kg−1) compared to the ARG monoculture (315 g ADF kg−1; 126 g CP kg−1). Benefits of intercropping perennial forages with cotton were maximized during the second year of growth, but future work is necessary to improve stand survival.
牧草-连作系统为生产者带来了重要的农艺和经济效益。然而,在美国东南部地区,很少有人关注将牧草纳入连作系统的问题。这项研究评估了在东南沿海平原的两个生产年中,在夏季棉花(Gossypium hirsutum L. )与多年生冷季豆科植物间作的可行性。处理方式包括杂草休耕、一年生黑麦草(ARG;Lolium multiflorum Lam.)单作、红三叶(RC;Trifolium pratense L.)和白三叶(WC;Trifolium repens L.)混作,以及 ARG、RC 和 WC 三种混作。地块于 2020 年秋季建立,在 2021 年和 2022 年 5 月之前种植牧草,之后对地块进行条耕并种植棉花。棉花管理中尽量少用除草剂,以保护多年生三叶草。结果表明,WC 比 RC 更持久(>40 株 m-2 对 <40株 m-2),在第二年春季,多年生三叶草对杂草的抑制水平与 ARG 单一种植(35 株杂草 m-2)相似。多年生三叶草与 ARG 混合后长得更高(4-5 厘米)。与 ARG 单作(315 g ADF kg-1;126 g CP kg-1)相比,在第二年春季三叶草与 ARG 混种降低了酸性洗涤纤维(ADF)浓度,增加了粗蛋白(CP)浓度(280 g ADF kg-1;167 g CP kg-1)。在棉花生长的第二年,多年生牧草与棉花间作的效益最大化,但今后仍需努力提高棉花的存活率。
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引用次数: 0
A review of intercropping systems in Western Canada 加拿大西部间作系统综述
IF 2.1 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-06-28 DOI: 10.1002/agj2.21622
Vengai Mbanyele, Rebecca Oiza Enesi, Lana Shaw, Linda Yuya Gorim
Intercropping is gaining interest from Western Canadian producers who are looking for information on how to incorporate intercrops into their production systems. This review summarizes agronomic research on intercropping from the last 40 years in Western Canada and discusses the potential challenges of integrating intercropping into existing crop rotations while identifying challenges and possible solutions. Reviewed literature indicates that several intercrop combinations have been tested in small plots involving up to four crops simultaneously grown, with over 60% comprising pulse–oilseed combinations followed by pulse–cereal combinations at ∼30%. The land equivalent ratio (LER) for pulse–oilseed and pulse–cereal averaged 1.11 and 1.13, respectively. Key agronomic factors that influenced LER in different intercrop combinations have been summarized, and the relationship of N and seeding rate with crop grain LER and partial land equivalent ratio has been assessed. While the relationship between N rate and LER was unclear in pulse–oilseed combinations such as pea (Pisum sativum L.)–canola (Brassica napus L.), LER decreased linearly with increasing N rate (p < 0.004) in pea–barley (Hordeum vulgare L.) intercrop. We highlighted that incorporating intercrops into current rotations will decrease crop rotation lengths with possible implications for disease management. There are many logistical challenges to intercropping but new technology may help producers to adapt.
加拿大西部的生产者对间作套种越来越感兴趣,他们正在寻找如何将间作套种纳入其生产系统的信息。本综述总结了加拿大西部过去 40 年来对间作套种的农艺学研究,并讨论了将间作套种纳入现有作物轮作中可能面临的挑战,同时确定了挑战和可能的解决方案。查阅的文献表明,在同时种植多达四种作物的小地块中测试了几种间作组合,其中 60% 以上是脉动-油籽组合,其次是脉动-谷物组合,比例为 30%。脉动-油籽和脉动-谷物的土地当量比(LER)平均分别为 1.11 和 1.13。总结了影响不同间作组合土地等值率的主要农艺因素,并评估了氮和播种率与作物籽粒土地等值率和部分土地等值率的关系。在豌豆(Pisum sativum L.)-油菜(Brassica napus L.)等豆类-油料作物组合中,氮含量与颖花率之间的关系并不明确,而在豌豆-大麦(Hordeum vulgare L.)间作中,颖花率随着氮含量的增加呈线性下降(p < 0.004)。我们强调,将间作作物纳入当前的轮作中将缩短轮作时间,并可能对疾病管理产生影响。间作存在许多后勤方面的挑战,但新技术可能有助于生产者适应这些挑战。
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引用次数: 0
Characterization of agronomic performance and sterility in triploid and diploid cannabinoid hemp 三倍体和二倍体大麻的农艺性能和不育特征
IF 2.1 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-06-27 DOI: 10.1002/agj2.21618
David H. Suchoff, Shannon Henriquez Inoa, George M. Stack, Alexander J. Wares, Stephen I. Snyder, Maylin J. Murdock, Jocelyn K. C. Rose, Lawrence B. Smart, Tara A. Caton, Robert C. Pearce
Cannabinoid hemp is a dioecious crop where pistillate plants are selectively grown to maximize cannabinoid yields. Errant pollination can reduce pistillate flower yields, cannabinoid concentrations, and lead to unmarketable flowers due to the presence of undesirable seeds. We compared pollen sensitivity and agronomic performance of diploid (2n = 2x = 20 chromosomes) and triploid (2n = 3x = 30 chromosomes) cannabinoid hemp in open‐field conditions. The high‐CBD variety Lifter and high‐CBG variety White CBG were evaluated in both their diploid and triploid counterparts in fields with and without pollen. Trials were conducted during the 2021 and 2022 field seasons in Kentucky, New York, and North Carolina (n = 6 site years). Triploids produced taller and wider plants than diploids; however, this did not result in yield differences in the pollen‐free environment. In the presence of pollen, triploid Lifter and White CBG produced 87% and 77% fewer seeds than their diploid counterparts, respectively. Increased seed production in diploids also resulted in a significant reduction of seed‐free biomass and cannabinoid concentrations compared to triploids. In the absence of pollen, we did not find any appreciable differences in seed‐free biomass or cannabinoid concentrations between triploids and diploids. Though not completely pollen‐insensitive, triploidy is an effective means to reduce seed production and improve yields and quality in cannabinoid hemp in the presence of pollen.
大麻是一种雌雄异株的作物,有选择地种植雌花以最大限度地提高大麻素产量。错误的授粉会降低雌花产量和大麻素浓度,并因不良种子的存在而导致花卉滞销。我们比较了二倍体(2n = 2x = 20 条染色体)和三倍体(2n = 3x = 30 条染色体)大麻在露地条件下的花粉敏感性和农艺性能。高CBD品种Lifter和高CBG品种White CBG分别在有花粉和无花粉的田间对其二倍体和三倍体对应品种进行了评估。试验于 2021 年和 2022 年田间季节在肯塔基州、纽约州和北卡罗来纳州进行(n = 6 个地点年)。与二倍体相比,三倍体的植株更高、更宽;但这并没有导致无花粉环境下的产量差异。在有花粉的情况下,三倍体 Lifter 和白色 CBG 产生的种子分别比二倍体少 87% 和 77%。与三倍体相比,二倍体种子产量的增加也导致无籽生物量和大麻素浓度的显著降低。在没有花粉的情况下,我们没有发现三倍体和二倍体的无籽生物量或大麻素浓度有任何明显差异。虽然三倍体并非对花粉完全不敏感,但在有花粉的情况下,三倍体是减少大麻籽产量、提高产量和质量的有效手段。
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引用次数: 0
Novel herbicide from an optimized Bacillus altitudinis D30202 solvent extract 从优化的高度芽孢杆菌 D30202 溶剂提取物中提取的新型除草剂
IF 2.1 3区 农林科学 Q2 AGRONOMY Pub Date : 2024-06-26 DOI: 10.1002/agj2.21623
Xiu‐hua Ma, Shuo Shen, Wei Li, Jian Wang
The herbicidal activity of the fermentation broth, filtrate, and extracts of Bacillus altitudinis D30202 was evaluated against seed germination and seedling growth of wild oat (Avena fatua L.). The bacterial culture filtrate exhibited greater inhibition of the wild oat weed than the fermentation broth. The filtrate was also extracted with organic solvents. The herbicidal potency of the extracts on the growth of wild oat plants was as follows: chloroform (100% inhibition of germination) > n‐butanol (93.8%) > ethyl acetate (66.7%) > petroleum ether (6.3%) > aqueous phase (2.1%); so the chloroform extract was evaluated further. It inhibited wild oat radicle growth by 100%, and plumule growth by 97.9%, while spraying young plants with a 5 mg/mL extract caused severe desiccation of the leaves, stalk wilting, and plant death. IC50 values for inhibition of plumule and radicle growth and germination were 0.64–0.72 mg/mL. Scanning electron microscopy and transmission electron microscopy revealed changes in the microstructure of the leaves and root tips, and degradation of organelles following chloroform extract treatment. The 5 mg/mL extract had no adverse impact on the growth or health of highland barley (Hordeum vulgare L.), corn (Zea mays), and broad bean (Vicia faba L.), indicating that this novel bioherbicide is suitable for control of wild oat weeds in the production of these food crops (while having a minor impact on the health of pea plants and being phytotoxic to wheat plants).
评估了高度芽孢杆菌 D30202 的发酵液、滤液和提取物对野生燕麦(Avena fatua L.)种子发芽和幼苗生长的除草活性。与发酵液相比,细菌培养滤液对野燕麦杂草的抑制作用更大。滤液也用有机溶剂提取。提取物对野燕麦植物生长的除草效力如下:氯仿(100% 抑制发芽);正丁醇(93.8%);乙酸乙酯(66.7%);石油醚(6.3%);水相(2.1%);因此对氯仿提取物进行了进一步评估。它对野生燕麦胚根生长的抑制率为 100%,对李子生长的抑制率为 97.9%,而用 5 毫克/毫升的提取物喷洒幼苗会导致叶片严重干枯、茎秆萎蔫和植株死亡。抑制梅花和胚根生长及发芽的 IC50 值为 0.64-0.72 毫克/毫升。扫描电子显微镜和透射电子显微镜显示,氯仿提取物处理后,叶片和根尖的微观结构发生变化,细胞器退化。5 毫克/毫升的提取物对高地大麦(Hordeum vulgare L.)、玉米(Zea mays)和蚕豆(Vicia faba L.)的生长和健康没有不良影响,表明这种新型生物除草剂适合在这些粮食作物的生产过程中控制野生燕麦杂草(同时对豌豆植物的健康有轻微影响,对小麦植物有植物毒性)。
{"title":"Novel herbicide from an optimized Bacillus altitudinis D30202 solvent extract","authors":"Xiu‐hua Ma, Shuo Shen, Wei Li, Jian Wang","doi":"10.1002/agj2.21623","DOIUrl":"https://doi.org/10.1002/agj2.21623","url":null,"abstract":"The herbicidal activity of the fermentation broth, filtrate, and extracts of <jats:italic>Bacillus altitudinis</jats:italic> D30202 was evaluated against seed germination and seedling growth of wild oat (<jats:italic>Avena fatua</jats:italic> L.). The bacterial culture filtrate exhibited greater inhibition of the wild oat weed than the fermentation broth. The filtrate was also extracted with organic solvents. The herbicidal potency of the extracts on the growth of wild oat plants was as follows: chloroform (100% inhibition of germination) &gt; <jats:italic>n</jats:italic>‐butanol (93.8%) &gt; ethyl acetate (66.7%) &gt; petroleum ether (6.3%) &gt; aqueous phase (2.1%); so the chloroform extract was evaluated further. It inhibited wild oat radicle growth by 100%, and plumule growth by 97.9%, while spraying young plants with a 5 mg/mL extract caused severe desiccation of the leaves, stalk wilting, and plant death. IC<jats:sub>50</jats:sub> values for inhibition of plumule and radicle growth and germination were 0.64–0.72 mg/mL. Scanning electron microscopy and transmission electron microscopy revealed changes in the microstructure of the leaves and root tips, and degradation of organelles following chloroform extract treatment. The 5 mg/mL extract had no adverse impact on the growth or health of highland barley (<jats:italic>Hordeum vulgare</jats:italic> L.), corn (<jats:italic>Zea mays</jats:italic>), and broad bean (<jats:italic>Vicia faba</jats:italic> L.), indicating that this novel bioherbicide is suitable for control of wild oat weeds in the production of these food crops (while having a minor impact on the health of pea plants and being phytotoxic to wheat plants).","PeriodicalId":7522,"journal":{"name":"Agronomy Journal","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141518220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Agronomy Journal
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