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Relationship between bovine brucellosis and production systems in Kashongi sub-county of Kiruhura-Uganda. 乌干达基鲁胡拉卡松吉县牛布鲁氏菌病与生产系统的关系
Mugizi
The present study aimed at determining the seroprevalence of bovine brucellosis and abortion across the major production systems practiced in Kashongi sub-county in 2006. A questionnaire was used to obtain breeding information and blood for testing exposure to Brucella abortus antigens was collected from a total of 258 cattle across the production systems practiced. There was a significant difference in the prevalence of bovine abortion among the 3 production systems and among breeds (p of animals from different herds tended to increase the prevalence of bovine brucellosis. It was concluded that intensification be  accompanied by good management practices and communally grazed herds be strictly of the resistant local breeds to reduce the risk of abortion.
本研究旨在确定2006年卡松吉县主要生产系统中牛布鲁氏菌病和流产的血清流行率。使用了一份调查问卷来获取育种信息,并从整个生产系统中共258头牛收集了用于检测流产布鲁氏菌抗原暴露的血液。牛流产率在3种生产体系之间存在显著差异,不同畜群的牛流产率往往会增加牛布鲁氏菌病的发病率。结论认为,强化放牧应辅以良好的管理措施,并严格采用具有抗性的地方品种,以减少流产风险。
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引用次数: 6
Effect of probiotics supplementation of cassava-groundnut cake meal based diet on the performance, nutrient digestibility and haematology in starting pigs 木薯花生饼粕基础饲粮中添加益生菌对育肥猪生产性能、营养物质消化率和血液学的影响
A. Fatufe
In a 56 days feeding trial, eighteen starting pigs with initial body weight of 6.78 ± 0.5 kg were assigned randomly to 2 dietary treatment groups of nine pigs each (3 pens of 3 pigs each). Diet I was the control, which was not supplemented with probiotics, while Diet II was supplemented with 0.05% probiotics (probiotics contained Saccharomyces cerevisae and Lactobacillus sporogenes) into the control diet at the expense of wheat bran. Diets and water were offered on ad libitum basis. Starting pigs responded nonsignificantly (p> 0.05) to probiotics supplementation in body weight gain, feed conversion ratio and feed intake. Similarly, wet and dry matter faecal output, dry matter, crude protein and crude fat digestibilities, packed cell volume, erythrocytes and leukocytes values were not significantly influenced by probiotics supplementation, whereas crude ash digestibility was significantly (p = 0.03) improved by probiotics supplementation. It was concluded that probiotics has no pronounced effect on the growth rate and nutrient digestibility of starting pigs in a cassava-groundnut cake meal based diet, however beneficial effect could be expected in terms of mineral metabolism. Similarly, the health status of starting pigs is not adversely affected by probiotics supplementation of cassava-groundnut cake meal based diet.
试验选取初始体重为6.78±0.5 kg的18头猪,随机分为2个饲粮处理组,每组9头猪(3个猪圈,每个猪圈3头猪)。饲粮I为对照,不添加益生菌,饲粮II在对照组饲粮中添加0.05%的益生菌(益生菌中含有酿酒酵母菌和产孢乳杆菌),同时不添加麦麸。日粮和水按量提供。饲粮中添加益生菌对仔猪增重、饲料系数和采食量无显著影响(p < 0.05)。同样,添加益生菌对湿、干物质排泄量、干物质消化率、粗蛋白质和粗脂肪消化率、堆积细胞体积、红细胞和白细胞值无显著影响,而粗灰分消化率显著提高(p = 0.03)。由此可见,益生菌对木薯花生饼粕基础饲粮的生长速率和营养物质消化率无显著影响,但对矿物质代谢有有益影响。同样,以木薯花生饼粕为基础的日粮中添加益生菌不会对仔猪的健康状况产生不利影响。
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引用次数: 0
Prevalence of Aeromonashydrophila and Yersinia Enterocolitica in some sea foods sold in sharkia governorate markets and the effects of heat treatments on their viabilities 沙尔基亚市售海产品中嗜水气单胞菌和结肠炎耶尔森菌的流行及热处理对其生存力的影响
S. E. Dien, Wm Enas, M. Sami, M. Hanan, El Lawendi, Masoumeh Saleh
Total of 120 samples of fresh mullet fish, frozen tilapia fillet and chilled shrimps (40 samples from each) were collected from Sharkia Governorate markets to assess the presence of Aeromonas hydrophila and Yersinia enterocolitica short methods. The results showed that the prevalence of Aeromonas spp. were 34 (85%), 12 (30%) and 28 (70%) for mullet fish, frozen tilapia fillet and shrimp samples respectively, the mean total colony count of this microorganism were 4.91 ±0.247, 4.383 ±0.463 and 4.44 ±0.125 log/ CFU in the same order. Aeromonas hydrophila, A. caviae and A. sobria were present in the examined samples, but A. hydrophila was the common species. Concerning Yersinia spp., it was detected in 26 (65%), 6 (15%) and 20 (50%) samples of mullet fish, frozen tilapia fillet and shrimp samples respectively, with the mean total colony counts of 5.142±0.353, 4.284 ±0.671 and 3.06±0.221 log/ CFU respectively in the same order. Y. enterocolitica, Y. fredrediksenii, Y. Intermedia and Y. Kristensenii were detected in the examined samples, while; Y. enterocolitica is the most predominant isolate. From the present investigation, both A. hydrophila and Y. enterocolitica are sensitive to heat treatment. Thus, good ripening of sea foods is adequate to control these microorganisms.
从Sharkia省市场共收集了120份新鲜鲻鱼、冷冻罗非鱼鱼片和冷冻虾(各40份)样本,以评估嗜水气单胞菌和小肠结肠炎耶尔森菌的存在。结果表明,在乌鱼、冷冻罗非鱼鱼片和对虾样品中,气单胞菌的检出率分别为34(85%)、12(30%)和28(70%),菌落总数依次为4.91±0.247、4.383±0.463和4.44±0.125 log/ CFU。检出嗜水气单胞菌、洞穴单胞菌和嗜水气单胞菌,但嗜水气单胞菌为常见种。乌鱼、冷冻罗非鱼片和对虾样品中检出耶尔森菌26例(65%)、6例(15%)和20例(50%),平均菌落总数依次为5.142±0.353、4.284±0.671和3.06±0.221 log/ CFU。检出小肠结肠炎耶氏菌、弗雷德氏耶氏菌、中间耶氏菌和克里斯滕森耶氏菌;小肠结肠炎菌是最主要的分离菌。从目前的调查来看,嗜水单胞菌和小肠结肠炎单胞菌都对热处理敏感。因此,成熟的海产品足以控制这些微生物。
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引用次数: 7
Influence of some expressive genes mutation on growth and body measurements of scavenging chickens in the dense humid forest of Cameroon 喀麦隆浓密潮湿森林中一些表达基因突变对食腐鸡生长和体型的影响
J. Fotsa, K. Poné, X. Rognon, M. Tixier-Boichard, Y. Manjeli, A. Bordas
Seven hundred and fifty one (751) mature local chickens reared in the dense humid forest zone of Cameroon were described to identify expressive genes mutation and their influences on growth and body measurements of birds in the rural environment of three provinces in Cameroon (Center, South and East). The major results showed that hens from the Center Province bearing CR*CR mutation (Crested) were heavier (87g; P PTI*PTI (feathered shank) and NA*NA (Naked neck) positively increased shank width but only in the Center Province by 0.23 cm and 0.19 cm compared with their wild alleles PTI*N and NA*N,  respectively. It was concluded that the expressive genes mutation could be associated with enhanced performance, although upgrading feeding, health and management conditions could optimize their expression.
在喀麦隆中部、南部和东部三省的农村环境中,对751只喀麦隆稠密潮湿林区饲养的成熟地方鸡进行了基因表达突变及其对鸟类生长和体型测量的影响。主要结果表明:中部省携带CR*CR突变(冠毛)的蛋鸡较重(87g);与野生等位基因PTI*N和NA*N相比,P PTI*PTI(羽柄)和NA*NA(裸颈)分别增加了0.23 cm和0.19 cm。综上所述,尽管改善饲养、卫生和管理条件可以优化表达基因的表达,但表达基因突变可能与提高生产性能有关。
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引用次数: 0
A synopsis of cattle performance in Zimbabwe’s ‘initial’ resettlement areas after land reforms and redistribution 津巴布韦土地改革和再分配后“最初”安置地区的牛的表现摘要
T. Chinuwo, P. Mugabe, I. Mpofu, S. Mulugeta, E. Timpong-Jones
Cattle performance was monitored over a two year period in a resettlement area, a spatial land-use product of Zimbabwe’s land reforms post-independence. A total of 30 farmers each owning at least 5 cattle in 3 selected villages of a 20 village resettlement scheme were targeted for the study. Participating village rangelands were assessed monthly for biomass yield in different physiognomic cover classes. Cattle in the study were monitored over a two year period for reproduction (calving rate and frequency, re-calving rates) and exit records (sales, slaughters, deaths, exchange, and buy-in) under farmer management conditions. Cattle weights, exit and reproduction records were analyzed as measures of performance. The study found low to medium calving rates (25-40%) and low re-calving rates (16 -26%), with diminished nutrition and low off-take (7.3% to 18.7%) as major impediments to cattle performance. Monthly cattle weights fluctuated with quantity of available grazing biomass, and were lowest during the dry months (September to December). Cows and heifers were affected more by diminished nutrition than steers. The study concluded that improving nutrition, increasing off-take and possibly availing bulls in resettlement areas were appropriate actions to increase cattle performance in the short to medium term, complimented by adequate veterinary practices.
在津巴布韦独立后土地改革的空间土地利用产物安置区内,对牛的表现进行了为期两年的监测。在20个村庄重新安置计划的3个选定村庄中,共有30名农民每人至少拥有5头牛。每个月对参与的村庄牧场在不同地貌覆盖等级下的生物量产量进行评估。在两年的时间里,研究人员监测了在农民管理条件下的牛的繁殖(产犊率和频率、再产犊率)和退出记录(销售、屠宰、死亡、交换和买入)。分析了牛重、退出和繁殖记录作为性能指标。研究发现,低至中等产犊率(25-40%)和低再产犊率(16 -26%),营养减少和低采食量(7.3%至18.7%)是牛生产性能的主要障碍。月牛体重随有效放牧生物量的变化而波动,在旱季(9 ~ 12月)最低。母牛和小母牛比阉牛更容易受到营养减少的影响。该研究的结论是,在适当的兽医实践的配合下,改善营养、增加摄取和可能在安置地区利用公牛是在中短期内提高牛生产性能的适当行动。
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引用次数: 0
Comparative susceptibility of vero and baby hamster kidney cell lines to PPR virus. 豚鼠和幼鼠肾细胞系对小反刍兽疫病毒的比较易感性。
B. Emikpe, O. Oyero, S. Akpavie
This study was undertaken to assess the comparative susceptibility of the different cell lines to PPRV using virus isolation by Vero and BHK cell lines from field samples. The inoculated BHK and Vero cells supported the growth of the virus with syncytia formation more commonly observed in the BHK cells while vacuolation and cell disintegration were more marked in Vero cells. When the virus was passaged from Vero cells to Vero cells, the CPE occurred on the fourth day while passaging from Vero cells to BHK cells, revealed CPE in three days. The onset of CPE and its pattern was earlier and more prominent in BHK than in Vero cells. This could be due to species difference in the origin of the two cell lines and the receptors for attachment and replication. As BHK cell line was not routinely used for primary isolation, results obtained in this study showed that the virus titres obtained are usually higher, the CPE produced clearer, and the onset of CPE development shorter hence it can the be good for primary isolation of PPRV.
本研究采用Vero和BHK细胞系从田间样本中分离病毒的方法,评估不同细胞系对PPRV的相对易感性。接种的BHK细胞和Vero细胞支持病毒生长,BHK细胞更常见的是合胞体形成,而Vero细胞更明显的是液泡形成和细胞解体。当病毒从Vero细胞传代到Vero细胞时,第4天出现CPE;从Vero细胞传代到BHK细胞时,第3天出现CPE。与Vero细胞相比,BHK细胞CPE的发病时间和模式更早、更突出。这可能是由于两种细胞系的起源和附着和复制受体的物种差异。由于BHK细胞系未常规用于原代分离,本研究结果表明,获得的病毒滴度通常较高,CPE产生更清晰,CPE发病时间较短,因此可以用于PPRV的原代分离。
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引用次数: 3
A serosurvey of Bovine Brucellosis in three cattle-rearing regions of Somaliland (Northern Somalia) 索马里兰(索马里北部)三个养牛区牛布鲁氏菌病血清调查
Y. Ghanem, A. Sawalhy, A. Saad, Ahemd H. Abdelkader, A. Heybe
A random survey to study the seroprevalence of bovine brucellosis has been carried out between July 2008 and December 2008 in three main districts of Somaliland (Awdal, Waqoyi Galbed and Togdheer) of northern Somalia. A total of 1668 bovine blood sera were randomly collected from 46 herd/flock of accessible unvaccinated male and female cattle to be analyzed. Rose Bengal Plate Test (RBPT) and indirect ELISA (I-ELISA) were used to screen all sera samples. The prevalence of brucellosis in the tested bovine sera were 5.5% (n=91), and 4.4% (n=74) by RBPT, and by I-ELISA respectively. There was a little difference regarding to sex of the prevalence between males and females in the overall studied districts as it was 5.0% and 3.8% in males by RBPT and I-ELISA respectively, while it was 5.8% and 5.0% in females respectively. There were little differences in prevalences of brucellosis among the studied districts which was highest in Awdal (6.5%) whereas in Waqoyi Galbeed (5.1%) and in Togdheer (5.5%) by RBPT, while it was in (5.1%), followed by Waqoyi Galbeed (4.1%), and Togdheer (4.1%) regions by (I-ELISA). There was no significant  difference in the seroprevalence according to the age.
2008年7月至2008年12月期间,在索马里兰北部的三个主要地区(Awdal、Waqoyi Galbed和Togdheer)进行了一项随机调查,以研究牛布鲁氏菌病的血清患病率。从46个可获得的未接种疫苗的公、母牛中随机抽取1668份牛血清进行分析。采用玫瑰孟加拉平板法(RBPT)和间接ELISA法(I-ELISA)对所有血清样本进行筛选。RBPT法和I-ELISA法检测的牛血清布鲁氏菌病检出率分别为5.5% (n=91)和4.4% (n=74)。在整个研究地区,男性和女性的患病率在性别上差异不大,RBPT和I-ELISA的男性患病率分别为5.0%和3.8%,而女性患病率分别为5.8%和5.0%。各区布鲁氏菌病患病率差异不大,RBPT法测定的最高地区为Awdal(6.5%),其次为Waqoyi Galbeed(5.1%)和Togdheer (5.5%), I-ELISA法测定的最高地区为Waqoyi Galbeed(5.1%)和Togdheer(4.1%)。不同年龄段的血清阳性率差异无统计学意义。
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引用次数: 2
A seroprevalence study of ovine and caprine brucellosis in three main regions of Somaliland (Northern Somalia) 索马里兰(索马里北部)三个主要地区绵羊和山羊布鲁氏菌病血清流行率研究
Y. Ghanem, A. Sawalhy, A. Saad, Ahemd H. Abdelkader, A. Heybe
A random survey to study the seroprevalence of ovine and caprine brucellosis was carried out between July 2008 and January 2009 in three main districts of arid and semiarid rearing regions of Somaliland. A total of 1938 sheep, and 1344 goats’ blood sera were randomly collected from 42 herd/flock of accessible unvaccinated sheep and goats to be analyzed. Rose Bengal Plate Test (RBPT) and indirect ELISA (I-ELISA) were used to screen all serum samples. At herd level, for sheep, a total 42 herd/flocks were examined, out of which, 2 (4.8%) were seronegative while 40 (95.2%) were seropositive by I-ELISA, 10 (23.8%) were seronegative and 32 (76.2%) seropositive by RBPT. At herd level, for goats, a total 42 herd/flocks were examined, out of which, 6 (14.3%) were seronegative while 36 (85.7%) were seropositive by I-ELISA, 11 (26.2%) were seronegative and 32 (73.8%) seropositive by RBPT. A non significant seroprevalence differences were found at herd level between the three studied districts. There was no significant difference in the prevalence of brucellosis between the different studied districts. This study throws a strong light on that sheep and goats should be included in a national program for control and eradication of brucellosis in Somaliland. Further epidemiological studies and identification of the Brucella biotypes involved is recommended.
2008年7月至2009年1月期间,在索马里兰干旱和半干旱养殖区的三个主要地区进行了一项研究绵羊和山羊布鲁氏菌病血清流行率的随机调查。从42个可获得的未接种疫苗的绵羊和山羊中随机采集1938只绵羊和1344只山羊的血清进行分析。采用玫瑰孟加拉平板试验(RBPT)和间接酶联免疫吸附试验(I-ELISA)对所有血清样本进行筛选。在羊群水平上,共检测了42只羊,其中2只(4.8%)血清阴性,40只(95.2%)血清阳性,10只(23.8%)血清阴性,32只(76.2%)血清阳性。在羊群水平上,共检测42只山羊,其中血清阴性6只(14.3%),I-ELISA血清阳性36只(85.7%),RBPT血清阴性11只(26.2%),血清阳性32只(73.8%)。在三个研究地区之间,发现畜群水平的血清阳性率差异不显著。不同地区的布鲁氏菌病患病率无显著差异。这项研究强烈表明,绵羊和山羊应该被纳入索马里兰控制和根除布鲁氏菌病的国家规划。建议进一步开展流行病学研究并确定所涉及的布鲁氏菌生物型。
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引用次数: 5
Prevalence of laminitis and the patterns of claw lesions in dairy cows in Nairobi and the Peri-Urban Districts 在内罗毕和城郊地区的奶牛板炎患病率和爪损的模式
J. Nguhiu-Mwangi, P. Mbithi, J. Wabacha, P. Mbuthia
Prospective study was carried out to determine the characteristics of claw lesions in dairy cattle in Nairobi and the peri-urban districts between December 2005 and May 2006. The gaits of 300 dairy cows in 29 zero-grazed and 3 pasture-grazed farms were evaluated and their claws examined for laminitis and other lesions. A retrospective study was also conducted on case records for dairy cattle foot lameness in the Large Animal Clinic of the University of Nairobi. Claws were also collected from abattoirs and examined for lesions. Data was analyzed with GENSTAT for Windows Discovery Edition 2. From the retrospective study, the prevalence of laminitic lesions was 0.79% while infective lesions particularly interdigital necrobacillosis and sole abscesses had prevalence of 35.7% and 11.1% respectively. In the prospective study, the prevalence of laminitis was 70.3%, sole bruising 45%, heel erosion 27.3%, white line separation 18% and double soles 17%. However, the total prevalence of infective claw lesions was 4.7%. Abattoir claws had lesions with similar trends as prospective survey. Current nutrition, housing and management practices that are adopted for increasing productivity and improving hygiene, may account for changes seen in claw lesion patterns of increased laminitis and decreased infective lesions in dairy cattle.
在2005年12月至2006年5月期间,对内罗毕和城郊地区奶牛爪损的特征进行了前瞻性研究。对29个零放牧牧场和3个放牧牧场的300头奶牛进行步态评价,并对其爪部进行足部炎等病变检查。还对内罗毕大学大型动物诊所的奶牛足跛病例记录进行了回顾性研究。还从屠宰场收集爪子并检查是否有病变。使用GENSTAT for Windows Discovery Edition 2分析数据。从回顾性研究来看,板状病变的患病率为0.79%,而感染性病变,特别是指间坏死杆菌病和足底脓肿的患病率分别为35.7%和11.1%。在前瞻性研究中,足板炎的患病率为70.3%,鞋底挫伤为45%,脚跟糜烂为27.3%,白线分离为18%,双底为17%。然而,感染性爪损的总患病率为4.7%。屠宰场爪子的病变趋势与前瞻性调查相似。目前为提高生产力和改善卫生而采用的营养、饲养和管理做法,可能解释了奶牛足板炎增加和传染性病变减少的爪损模式的变化。
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引用次数: 4
Experimental studies on some parasites of public health impoetance in market fish. 市售鱼类中几种对公共卫生有害的寄生虫的实验研究。
E. Sahar, A. Nagwa, M. Hanan
A total of 500 fish (250 Tilapia sp. and 250 Clarias lazera were  collected from fish markets at Zagazige city in Sharkia governorate. The encysted metacercariae were detected in Tilapia sp. 84.8% and in Clarias lazera 86.8% of the examined fish. The adult worm were collected after experimental infection of puppies with encysted metacercariae as Heterophyes heterophyes, Heterophyes aequalis, Prohemistomum vivax , Mesostephanus appendiculatus and Mesostephanus flapi. The control of these metacercariae were attempted by freezing at-180C for 48 hour, grilling for 10 minutes and frying for 5 minutes. The aim of the present investigation is to study the public health importance of encysted metacercariae in (Tilapia sp. and Clarias lazera)by experimental infection of puppies and identification of recovered adult. Moreover, the effect of some treatment methods rendering the infected fish safe for consumption.
在沙尔基亚省扎加齐格市的鱼市共收集了500条鱼(250条罗非鱼和250条克拉拉鱼)。罗非鱼检出率为84.8%,克拉氏鱼检出率为86.8%。实验感染包囊囊蚴幼犬后,采集成虫,分别为异盲异眼、平异眼异眼、间日异眼、尾盲中棘和flapi中棘。用180℃冷冻48小时、烤10分钟、煎5分钟的方法控制这些囊蚴。本研究旨在通过对幼犬的实验感染和对成虫的鉴定,探讨罗非鱼(罗非鱼属)囊蚴感染的公共卫生意义。此外,一些处理方法的效果使受感染的鱼可以安全食用。
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引用次数: 2
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Bulletin of animal health and production in Africa. Bulletin des sante et production animales en Afrique
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