Pub Date : 2011-03-08DOI: 10.4314/BAHPA.V58I3.64214
B. Emikpe, S. Akpavie
Mannheimiosis due to Mannheimia hemolytica was experimental y induced in 15 six -month-old West african dwarf goats by intratracheal inoculation of 1 x 109 colony-forming units of four hour log-phase Mannheimia hemolytica serotype A:2 with five goats serving as controls. The clinical signs were observed and two goats were euthanized at predetermined intervals for bacteriological and pathological investigations on tissues col ected using standard techniques. The clinical signs in order of manifestation were anorexia, pyrexia, dyspnea, nasal discharge, recumbency and death. The prominent lesions observed at necropsy were pulmonary congestion and edema with consolidation and pleural adhesions of the apical lobes, fibrinous pleurisy as wel as, petechial and ecchymotic hemorrhages of skeletal muscles. The average percentage lung consolidation for the infection was 10.0% with no significant sex variation (P>0.05) and the right lung was more affected (p Keywords: Goat – Pneumonia – Mannheimia hemolytica, Experimental infection – Nigeria. Mannheimiosis causee par Mannheimia hemolytica a ete provoque experimentalement chez 15 chevres naines d’Afrique de l’ouest âgees de six mois par inoculation intra tracheale de 1 x 109 d’unites formant colonies de phase de croissance logarithmique de quatre heures de Mannheimia hemolytica de type serologique A:2 avec cinq chevres servant de temoins. Les signes cliniques ont ete observes et deux chevres ont ete euthanasiees a des interval es predeterminees pour faire des analyses bacteriologiques et pathologiques sur les tissus preleves a l’aide des techniques usuel es. Les signes cliniques par ordre d’apparition etaient l’anorexie, la pyrexie, la dyspnee, l’ecoulement nasal, le decubitus et la mort. Les observations importantes faites a l’autopsie etaient la congestion pulmonaire, un oedeme avec une hepatisation et des adherences pleurales des lobes apicaux, la pleuresie fibrineuse ainsi que des petechies et ecchymoses hemorragiques des muscles squelettiques. Le pourcentage moyen de l’hepatisation des poumons pour l’infection etait de 10,0% sans variations remarquables entre les sexes (P>0,05) et le poumon droit etait le plus touche (p Mots cles: Chevres, pneumonie, Mannheimia hemolytica A2, infection experimentale
本文以5只山羊为对照，对15只6月龄西非矮山羊气管内接种1 × 109个4小时溶血性曼海姆病血清型A:2菌落形成单位，实验性诱导溶血性曼海姆病。观察临床症状，并在预定的时间间隔对两只山羊实施安乐死，使用标准技术对组织进行细菌学和病理学检查。临床表现为厌食、发热、呼吸困难、流鼻液、躺卧、死亡。尸检中观察到的突出病变是肺充血和水肿，并伴有根尖叶的实变和胸膜粘连，纤维性胸膜炎以及骨骼肌瘀点和淤血出血。感染的平均肺实变率为10.0%，性别差异无统计学意义(P < 0.05)，右肺受影响更大(P关键词:山羊-肺炎-溶血性肝炎，实验性感染-尼日利亚。1 × 109 d 'unites形成菌落de phase de croismique de quresmique de quresres de blood ologique a:2个平均5个chresserserde temoins。临床症状不包括观察，两种疾病不包括安乐死，预先确定的时间间隔不包括分析，细菌学和病理学不包括组织疾病，通常技术不包括治疗。临床症状表现为:厌食、发热、呼吸困难、鼻部疼痛、卧位和鼻部疼痛。主要表现为肺充血、肝纤维化、肺顶叶胸膜粘连、胸膜纤维蛋白升高、肺内膜淤血、肌肉挤压性出血等。感染人群肝炎发生率为10.0%，无性别差异(P < 0.05);感染人群肝炎发生率为10.0%，无性别差异(P < 0.05);感染人群肝炎发生率为10.0%，无性别差异(P < 0.05);感染人群肝炎发生率为10.0%，无性别差异(P < 0.05)
{"title":"The clinical and Pathological features of experimental Mannheimia Hemolytica A2 infectionin West African Dwarf Goats.","authors":"B. Emikpe, S. Akpavie","doi":"10.4314/BAHPA.V58I3.64214","DOIUrl":"https://doi.org/10.4314/BAHPA.V58I3.64214","url":null,"abstract":"Mannheimiosis due to Mannheimia hemolytica was experimental y induced in 15 six -month-old West african dwarf goats by intratracheal inoculation of 1 x 109 colony-forming units of four hour log-phase Mannheimia hemolytica serotype A:2 with five goats serving as controls. The clinical signs were observed and two goats were euthanized at predetermined intervals for bacteriological and pathological investigations on tissues col ected using standard techniques. The clinical signs in order of manifestation were anorexia, pyrexia, dyspnea, nasal discharge, recumbency and death. The prominent lesions observed at necropsy were pulmonary congestion and edema with consolidation and pleural adhesions of the apical lobes, fibrinous pleurisy as wel as, petechial and ecchymotic hemorrhages of skeletal muscles. The average percentage lung consolidation for the infection was 10.0% with no significant sex variation (P>0.05) and the right lung was more affected (p Keywords: Goat – Pneumonia – Mannheimia hemolytica, Experimental infection – Nigeria. Mannheimiosis causee par Mannheimia hemolytica a ete provoque experimentalement chez 15 chevres naines d’Afrique de l’ouest âgees de six mois par inoculation intra tracheale de 1 x 109 d’unites formant colonies de phase de croissance logarithmique de quatre heures de Mannheimia hemolytica de type serologique A:2 avec cinq chevres servant de temoins. Les signes cliniques ont ete observes et deux chevres ont ete euthanasiees a des interval es predeterminees pour faire des analyses bacteriologiques et pathologiques sur les tissus preleves a l’aide des techniques usuel es. Les signes cliniques par ordre d’apparition etaient l’anorexie, la pyrexie, la dyspnee, l’ecoulement nasal, le decubitus et la mort. Les observations importantes faites a l’autopsie etaient la congestion pulmonaire, un oedeme avec une hepatisation et des adherences pleurales des lobes apicaux, la pleuresie fibrineuse ainsi que des petechies et ecchymoses hemorragiques des muscles squelettiques. Le pourcentage moyen de l’hepatisation des poumons pour l’infection etait de 10,0% sans variations remarquables entre les sexes (P>0,05) et le poumon droit etait le plus touche (p Mots cles: Chevres, pneumonie, Mannheimia hemolytica A2, infection experimentale","PeriodicalId":75643,"journal":{"name":"Bulletin of animal health and production in Africa. Bulletin des sante et production animales en Afrique","volume":"58 1","pages":"253-261"},"PeriodicalIF":0.0,"publicationDate":"2011-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70487550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-08DOI: 10.4314/BAHPA.V58I4.64229
S. Younis, Hm El Basheir, Ambar Ahmed, Y. Elmansoury, Amani Magid, Ayman Osman
This study was carried out to assess and quantify the post- treatments changes in cellular responses in calves naturally infected with microfilariae (mff) of Onchocerca gutturosa. Peripheral blood cells (PBC) from Onchocerca gutturosa - infected calves (n=9) as well as from uninfected ones (n=3) were subjected to an in vitro cyto-adherence to microfilariae (mff) and lymphocyte proliferation in response to two different antigens. Infected groups of three calves each, were treated with either Chloroquine (200mg i/m daily for 7 days), a single s/c dose of Ivermectin (150 μg / kg body weight) or standard dose of Artemether (160 mg i/m for three successive days). Two weeks post- treatments the same assays were repeated. Results showed that the cyto-adherence to mff was greater in cultures with sera from infected calves whereby the adherent cells covered 17 – 20.9% of the mff body surface, compared to a weak adherence to mff in cultures with control sera from uninfected calves (3.4%). Treatment resulted in a significant (P Key words: Onchocerca gutturosa, Peripheral blood cells, vitro cyto-adherence, calves Cette etude a ete realisee pour evaluer et quantifier les changements post-traitements dans les reactions cellulaires chez les veaux infectes naturellement par des microfilaires (mmf) de l’onchocercose gutturosa. Les cellules sanguines peripheriques (CSP) obtenues a partir de veaux infectes par l’onchocercose gutturosa - (n = 9), ainsi que de veaux non infectes (n = 3) ont ete soumises a une cyto-adherence invitro aux microfilaires (mmf) et a la proliferation des lymphocytes en reaction a deux antigenes differents. Les groupes atteints de trois veaux chacun, ont ete traites soit par la chloroquine (200 mg i / m par jour pendant 7 jours), une dose unique s / c de l’ivermectine (150 ug / kg de poids corporel) ou une dose standard de l’artemether (160 i mg / m pendant trois jours successifs). Deux semaines apres ces traitements, les memes essais ont ete repetes. Les resultats obtenus montrent que la cyto-adherence des microfilaires etait plus grande dans les cultures faites avec des serums de veaux infectes, la ou les cellules adherentes couvraient de 17 a 20,9% de la surface du corps des mmf, comparativement a une faible adherence aux mmf dans les cultures avec des serums de controle de veaux sains ( 3,4%). Le traitement a entraine une augmentation significative de l’adherence a la surface des mmf (P ete plutot faible dans les groupes infectes avec un indice de stimulation (SI) variant entre 1,70 a 2,15 ; alors que chez les veaux infectes elle etait comprise entre 1,13 a 1,66. Apres les traitements, la proliferation des lymphocytes n’a montre aucun changement dans l’indice de stimulation (SI) et celle-ci se situait entre 1,60 a 2,13. Il a ete conclu que: le traitement par l’ivermectine, la chloroquine ou l’artemether ont ameliore considerablement l’adherence cellulaire a la surface des ffm sur les serums stimules alors que, son effet sur les reponses
本研究旨在评估和量化自然感染绒毛盘尾丝虫(mff)的小牛在处理后细胞反应的变化。本研究对感染盘尾丝虫的小牛(n=9)和未感染盘尾丝虫的小牛(n=3)外周血细胞(PBC)进行了体外细胞粘附微丝蚴(mff)和淋巴细胞增殖对两种不同抗原的反应。每组3头小牛分别接受氯喹(每天200mg /m，连续7天)、单剂量伊维菌素(150 μg / kg体重)或标准剂量蒿甲醚(160 mg /m，连续3天)治疗。治疗两周后重复同样的测定。结果表明，与未感染小牛的对照血清培养的mff粘附较弱(3.4%)相比，用感染小牛的血清培养的mff粘附细胞更强，粘附细胞覆盖了mff体表的17 - 20.9%。关键词:盘尾丝虫病，外周血细胞，体外细胞粘附，犊牛，体外细胞粘附，体外细胞粘附，体外细胞粘附，体外细胞粘附，体外细胞粘附，体外细胞粘附，体外细胞粘附，体外细胞粘附，体外细胞粘附，体外细胞粘附，体外细胞粘附，体外细胞粘附，体外细胞粘附，体外细胞粘附外周血源性细胞(CSP)获得了1组小鼠感染(n = 9)， 1组小鼠感染(n = 3)， 1组小鼠感染(n = 3)， 1组细胞粘附性体外微丝细胞(mmf)和2组不同抗原反应的淋巴细胞增殖。三组药物分别为三联剂、三联剂氯喹(200毫克/立方米/小时，连续7小时)、一组标准剂量的甲醚(150毫克/公斤，连续3小时)和一组标准剂量的甲醚(160毫克/立方米，连续3小时)。双模组表现为双特征，双模组表现为双重复。结果obtenus montrent乘缆车cyto-adherence des microfilaires定律更大在文化做了用des血清de veaux感染,拉欧莱斯小房附着couvraient de 17 20 9% de la表面du陆战队des mmf, comparativement有一个faible依从性辅助mmf在文化用des血清de controle de veaux理科(3 4%)。结果表明:1、1、2、3组感染均有显著性差异，分别为1、70和2、15组;该中心包括1,13至1,66号中心。治疗后，淋巴细胞的增殖、细胞内细胞的变化、刺激指数(SI)和细胞内细胞的变化情况[1,60]和[2,13]。结果表明:甲维菌素、氯喹和甲氧基甲醚均能显著改善细胞粘附性和细胞表面粘附性，对血清、淋巴细胞、淋巴细胞增殖的抑制作用不显著。主要细胞:盘尾丝虫病、外周血细胞、体外细胞粘附、静脉
{"title":"Post-treatment cyto-adherence and lymphocytes proliferation in Onchocerca gutturosa infected-zebu calves.","authors":"S. Younis, Hm El Basheir, Ambar Ahmed, Y. Elmansoury, Amani Magid, Ayman Osman","doi":"10.4314/BAHPA.V58I4.64229","DOIUrl":"https://doi.org/10.4314/BAHPA.V58I4.64229","url":null,"abstract":"This study was carried out to assess and quantify the post- treatments changes in cellular responses in calves naturally infected with microfilariae (mff) of Onchocerca gutturosa. Peripheral blood cells (PBC) from Onchocerca gutturosa - infected calves (n=9) as well as from uninfected ones (n=3) were subjected to an in vitro cyto-adherence to microfilariae (mff) and lymphocyte proliferation in response to two different antigens. Infected groups of three calves each, were treated with either Chloroquine (200mg i/m daily for 7 days), a single s/c dose of Ivermectin (150 μg / kg body weight) or standard dose of Artemether (160 mg i/m for three successive days). Two weeks post- treatments the same assays were repeated. Results showed that the cyto-adherence to mff was greater in cultures with sera from infected calves whereby the adherent cells covered 17 – 20.9% of the mff body surface, compared to a weak adherence to mff in cultures with control sera from uninfected calves (3.4%). Treatment resulted in a significant (P Key words: Onchocerca gutturosa, Peripheral blood cells, vitro cyto-adherence, calves Cette etude a ete realisee pour evaluer et quantifier les changements post-traitements dans les reactions cellulaires chez les veaux infectes naturellement par des microfilaires (mmf) de l’onchocercose gutturosa. Les cellules sanguines peripheriques (CSP) obtenues a partir de veaux infectes par l’onchocercose gutturosa - (n = 9), ainsi que de veaux non infectes (n = 3) ont ete soumises a une cyto-adherence invitro aux microfilaires (mmf) et a la proliferation des lymphocytes en reaction a deux antigenes differents. Les groupes atteints de trois veaux chacun, ont ete traites soit par la chloroquine (200 mg i / m par jour pendant 7 jours), une dose unique s / c de l’ivermectine (150 ug / kg de poids corporel) ou une dose standard de l’artemether (160 i mg / m pendant trois jours successifs). Deux semaines apres ces traitements, les memes essais ont ete repetes. Les resultats obtenus montrent que la cyto-adherence des microfilaires etait plus grande dans les cultures faites avec des serums de veaux infectes, la ou les cellules adherentes couvraient de 17 a 20,9% de la surface du corps des mmf, comparativement a une faible adherence aux mmf dans les cultures avec des serums de controle de veaux sains ( 3,4%). Le traitement a entraine une augmentation significative de l’adherence a la surface des mmf (P ete plutot faible dans les groupes infectes avec un indice de stimulation (SI) variant entre 1,70 a 2,15 ; alors que chez les veaux infectes elle etait comprise entre 1,13 a 1,66. Apres les traitements, la proliferation des lymphocytes n’a montre aucun changement dans l’indice de stimulation (SI) et celle-ci se situait entre 1,60 a 2,13. Il a ete conclu que: le traitement par l’ivermectine, la chloroquine ou l’artemether ont ameliore considerablement l’adherence cellulaire a la surface des ffm sur les serums stimules alors que, son effet sur les reponses ","PeriodicalId":75643,"journal":{"name":"Bulletin of animal health and production in Africa. Bulletin des sante et production animales en Afrique","volume":"58 1","pages":"349-354"},"PeriodicalIF":0.0,"publicationDate":"2011-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70487901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-08DOI: 10.4314/BAHPA.V58I4.64231
A. Oloye, M. Oyeyemi, S. Olurode, M. Durosinmi
Eight healthy west African dwarf rams aged between 1.5 and 2.5 years weighing between 13 and 18 kg were assigned randomly into groups for right and left testicular orchidectomy. The Hemi-orchiidectomy was performed after three weeks of quarantine and testicular characteristics of excised testes examined. Thereafter, for a period of seven weeks, spermiograms were studied and recorded after which intact testes were removed and responsiveness to hemiorchidectomy studied. Basal spermiogram, gotten from all the animals before hemiorchidectomy served as control while comparison were made between testes removed during hemiorchidectomy and the intact testis removed thereafter. Compensatory hypertrophy was observed in all the animals after the procedure in the two groups. However this compensation was not of significant variation comparing animals assigned to left testicular orchidectomy with those for right testicular orchidectomy, (p hence their recommendation for use in the event of accidental loss, irreversible atrophy or degeneration of a single testis. Key words : Hemiorchidectomy, Spermiogram, Testicular Characteristics, West African Dwarf, Ram. Huit beliers nains d’Afrique de l’Ouest en bonne sante d’âge variant entre 1,5 et 2,5 ans et pesant entre 13 et 18 kg, ont ete repartis au hasard en groupes pour une orchidectomie des testicules droit et gauche. L’hemiorchidectomie a ete realisee apres trois semaines de quarantaine et les caracteristiques des testicules excises ont ete examines. Sur une periode de sept semaines, les spermogrammes ont ete par la suite enregistres et etudies ; apres quoi les testicules intacts ont ete enleves et leur reactivite aux hemiorchidectomies etudiee. Le spermogramme de base obtenu a partir de tous les animaux avant hemiorchidectomie a servi de temoin, tandis qu’une comparaison a ete faite entre les testicules enleves pendant l’hemiorchidectomie et le testicule intact qui a ete retire par la suite. L’hypertrophie compensatrice a ete observee chez tous les animaux apres cette procedure dans les deux groupes. Toutefois, cette compensation n’a pas varie de maniere significative en comparant les animaux soumis a l’orchidectomie testiculaire gauche avec ceux de orchidectomie testiculaire droite, (p testicule unique. Mots cles: hemiorchidectomie, spermogramme, testicules, Beliers nains d’Afrique de l’Ouest
选取8只年龄在1.5 ~ 2.5岁、体重在13 ~ 18公斤之间的健康西非矮公羊，随机分为两组，分别进行左右睾丸和睾丸切除术。在隔离三周后进行半睾丸切除术，并检查切除睾丸的特征。此后，在7周的时间里，研究并记录精子图，之后取出完整的睾丸，并研究对半睾丸切除术的反应。以所有动物半睾丸切除术前的基础精子图为对照，将半睾丸切除术后切除的睾丸与切除后的完整睾丸进行比较。两组动物术后均出现代偿性肥厚。然而，将左睾丸切除术与右睾丸切除术的动物进行比较，这种补偿没有显著差异，因此他们建议在发生意外损失，单个睾丸不可逆转萎缩或变性的情况下使用。关键词:半睾丸切除术，精子图，睾丸特征，西非侏儒，公羊。Huit认为，在非洲西部地区，有1、5、2、5个品种的品种为1、5、2、5个品种的品种为13、18公斤的品种，有3个品种的品种为1、5、2、5个品种。半睾丸切除术是一种有效的治疗方法，它可以有效地保护睾丸运动和检查的特征。在6个月的时间里，精子图和其他研究的精子图是一致的;研究了半睾丸切除术后睾丸完整程度的变化及其对神经反应的影响。半睾丸切除术前和半睾丸切除术前的精子图与未切除的精子图比较，并与未切除的精子图和未切除的精子图比较。肥厚代偿在两组中观察到，两组的肥厚代偿在两组的肥厚代偿中观察到。因此，睾丸代偿在不同的个体中具有不同的意义，在不同的个体中具有不同的意义，在不同的个体中具有不同的意义，在不同的个体中具有不同的意义。Mots:半睾丸切除术，精子检查，睾丸，Beliers nains d 'Afrique de l ' west
{"title":"Effect of Hemiorchidectomy on Spermiogram and Testicular Characteristics of West African Dwarf Ram.","authors":"A. Oloye, M. Oyeyemi, S. Olurode, M. Durosinmi","doi":"10.4314/BAHPA.V58I4.64231","DOIUrl":"https://doi.org/10.4314/BAHPA.V58I4.64231","url":null,"abstract":"Eight healthy west African dwarf rams aged between 1.5 and 2.5 years weighing between 13 and 18 kg were assigned randomly into groups for right and left testicular orchidectomy. The Hemi-orchiidectomy was performed after three weeks of quarantine and testicular characteristics of excised testes examined. Thereafter, for a period of seven weeks, spermiograms were studied and recorded after which intact testes were removed and responsiveness to hemiorchidectomy studied. Basal spermiogram, gotten from all the animals before hemiorchidectomy served as control while comparison were made between testes removed during hemiorchidectomy and the intact testis removed thereafter. Compensatory hypertrophy was observed in all the animals after the procedure in the two groups. However this compensation was not of significant variation comparing animals assigned to left testicular orchidectomy with those for right testicular orchidectomy, (p hence their recommendation for use in the event of accidental loss, irreversible atrophy or degeneration of a single testis. Key words : Hemiorchidectomy, Spermiogram, Testicular Characteristics, West African Dwarf, Ram. Huit beliers nains d’Afrique de l’Ouest en bonne sante d’âge variant entre 1,5 et 2,5 ans et pesant entre 13 et 18 kg, ont ete repartis au hasard en groupes pour une orchidectomie des testicules droit et gauche. L’hemiorchidectomie a ete realisee apres trois semaines de quarantaine et les caracteristiques des testicules excises ont ete examines. Sur une periode de sept semaines, les spermogrammes ont ete par la suite enregistres et etudies ; apres quoi les testicules intacts ont ete enleves et leur reactivite aux hemiorchidectomies etudiee. Le spermogramme de base obtenu a partir de tous les animaux avant hemiorchidectomie a servi de temoin, tandis qu’une comparaison a ete faite entre les testicules enleves pendant l’hemiorchidectomie et le testicule intact qui a ete retire par la suite. L’hypertrophie compensatrice a ete observee chez tous les animaux apres cette procedure dans les deux groupes. Toutefois, cette compensation n’a pas varie de maniere significative en comparant les animaux soumis a l’orchidectomie testiculaire gauche avec ceux de orchidectomie testiculaire droite, (p testicule unique. Mots cles: hemiorchidectomie, spermogramme, testicules, Beliers nains d’Afrique de l’Ouest","PeriodicalId":75643,"journal":{"name":"Bulletin of animal health and production in Africa. Bulletin des sante et production animales en Afrique","volume":"58 1","pages":"365-368"},"PeriodicalIF":0.0,"publicationDate":"2011-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70487986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-08DOI: 10.4314/BAHPA.V58I3.64207
MC Kadja, M. Kpodékon, Y. Kane, K. Tchassou, Y. Kaboret, J. Maini, B. Taminiau
Surveys conducted in Senegal have shown a strong association of staphylococci with subclinical mastitis in dairy cows. This study aimed to characterise Staphylococcus aureus strains identified in the dairy farms in Dakar. Of a total of 244 Staphylococcus spp isolates col ected from 135 lactating cows with subclinical mastitis at six dairy herds in peri-urban region of Dakar, 109 S. aureus strains were isolated and identified using phenotypic methods. Using PCR, genes of thermonuclease and major capsular type 5 and 8 antigens were identified respectively in 98.17%, 93.58% and 1.84% of S. aureus strains. Similarly, the spa gene was found in 68.81% of S. aureus strains, luk D (62.39%) and luk S (0.92%). Among the virulence genes sought, spa, Luk S and Luk D genes were found respectively in 68.81%, 62.39% and 0.92% strains of S. aureus. However, the lukF, lukM and tst1 genes were absent in al isolated S. aureus. Among the six enterotoxins analysed, none of the S. aureus harboured the genes sea, sec and seh. Only the seb, sei and sej were found respectively at rates of 2.75%, 3.67% and 26.6%. Final y, the gene for resistance to methicil in was found in 2.75% of the strains isolated. The analysis of the association between virulence genes and the presence of subclinical mastitis showed the importance of genes Spa lukD and SEJ. Antibiotic resistance testing revealed a good sensitivity of S. aureus to most of the antibiotics tested. This study showed the low virulence of the S aureus strains isolated. Keywords: Dairy cows - Subclinical mastitis - Staphylococcus aureus - PCR - Dakar Des enquetes menees au Senegal ont montre la forte correlation des staphylocoques aux mammites subcliniques chez les vaches laitieres. La presente etude avait pour but de caracteriser les souches de Staphylococcus aureus identifies dans les fermes laitieres a Dakar. Sur un total de 244 isolats de Staphylococcus spp isoles de 135 vaches laitieres a mammites subcliniques dans six troupeaux de vaches laitieres en zone peri-urbaine de Dakar, 109 souches de S. aureus ontete isolees et identifiees par des methodes phenotypiques. Par PCR, les genes de la thermonuclease et des antigenes capsulaires majeurs 5 et 8 ont ete identifies respectivement chez 98,17%, 93.58% et 1,84% des souches de S. aureus. Parmi les genes de virulence recherches, les genes spa, Luk D et Luk S ont ete retrouves respectivement chez 68,81%, 62,39% et 0,92% des souches de S. aureus. En revanche, les genes lukF, lukM et tst1 etaient absents. Parmi les six enterotoxines recherchees, sec et seh etaient absentes, seb, sei et sej ont ete retrouvees respectivement chez 2,75%, 3,67% et de 26,6% des souches. Enfin, le gene de resistance a la methicil ine a ete retrouve dans 2,75% des souches isolees. L’analyse de l’association entre les genes de virulence et la presence de mammites subcliniques a montre l’importance des genes spa, lukD et sej. Une bonne sensibilite de S. aureus a ete notee a la plupart des antibiotique
在塞内加尔进行的调查显示，葡萄球菌与奶牛的亚临床乳腺炎密切相关。本研究旨在鉴定在达喀尔奶牛场发现的金黄色葡萄球菌菌株的特征。从达喀尔近郊6个奶牛场135头患有亚临床乳腺炎的泌乳奶牛中分离出244株葡萄球菌，其中109株金黄色葡萄球菌采用表型方法进行了分离鉴定。PCR检测结果显示，金黄色葡萄球菌热核酸酶基因在98.17%、93.58%和1.84%中被检出，主要荚膜5型和8型抗原基因被检出。同样，spa基因在68.81%的金黄色葡萄球菌、luk D(62.39%)和luk S(0.92%)中检出。在所寻找的毒力基因中，spa、Luk S和Luk D基因分别存在于68.81%、62.39%和0.92%的金黄色葡萄球菌菌株中。而lukF、lukM和tst1基因在所有分离的金黄色葡萄球菌中均不存在。在分析的6种肠道毒素中，没有金黄色葡萄球菌含有sea、sec和seh基因。只有seb、sei和sej的阳性率分别为2.75%、3.67%和26.6%。最后，在2.75%的分离菌株中发现了对甲氧敌敌畏的抗性基因。毒力基因与亚临床乳腺炎的相关性分析表明，基因Spa lud和SEJ的重要性。抗生素耐药试验显示金黄色葡萄球菌对大多数所测抗生素具有良好的敏感性。本研究表明，分离的金黄色葡萄球菌菌株具有低毒力。关键词:奶牛-亚临床乳腺炎-金黄色葡萄球菌- PCR -塞内加尔达喀尔乳糜炎与乳糜炎的相关性本文介绍了一种金黄色葡萄球菌在达喀尔的鉴定方法。共分离出244株葡萄球菌分离株、135株乳齿动物分离株和6株达喀尔近郊地区分离株、109株金黄色葡萄球菌分离株，鉴定出不同方法的表型。PCR结果显示，热核酶基因和荚膜主要抗原5和8个基因分别鉴定出金黄色葡萄球菌的98、17%、93.58%和1.84%。Parmi les genes de毒力研究、les genes spa、Luk D和Luk S ont et分别为68、81%、62、39%和0、92%。即便如此，基因lukF、lukM等也会导致基因缺失。Parmi研究了6种肠毒素，其中2种肠毒素、2种肠毒素、3种肠毒素、2种肠毒素、3种肠毒素、2种肠毒素、3种肠毒素、3种肠毒素、6种肠毒素分别为2、75%、3、67%、26、6%。结果显示，抗药基因和抗药菌株的回收率为2.75%。我分析了我的关联中心，这些基因的毒性和哺乳动物亚临床的存在，以及我的重要性的基因spa, lud等。一个对金黄色葡萄球菌敏感的骨质疏松症在抗生素治疗后被发现。对金黄色葡萄球菌分离株的毒力进行了研究。动物:乳糜泻-乳齿亚群-金黄色葡萄球菌- PCR -达喀尔
{"title":"Typing of Staphylococcus aureus strains isolated from milk cows with subclinical mastitis in Dakar, Senegal Kadja","authors":"MC Kadja, M. Kpodékon, Y. Kane, K. Tchassou, Y. Kaboret, J. Maini, B. Taminiau","doi":"10.4314/BAHPA.V58I3.64207","DOIUrl":"https://doi.org/10.4314/BAHPA.V58I3.64207","url":null,"abstract":"Surveys conducted in Senegal have shown a strong association of staphylococci with subclinical mastitis in dairy cows. This study aimed to characterise Staphylococcus aureus strains identified in the dairy farms in Dakar. Of a total of 244 Staphylococcus spp isolates col ected from 135 lactating cows with subclinical mastitis at six dairy herds in peri-urban region of Dakar, 109 S. aureus strains were isolated and identified using phenotypic methods. Using PCR, genes of thermonuclease and major capsular type 5 and 8 antigens were identified respectively in 98.17%, 93.58% and 1.84% of S. aureus strains. Similarly, the spa gene was found in 68.81% of S. aureus strains, luk D (62.39%) and luk S (0.92%). Among the virulence genes sought, spa, Luk S and Luk D genes were found respectively in 68.81%, 62.39% and 0.92% strains of S. aureus. However, the lukF, lukM and tst1 genes were absent in al isolated S. aureus. Among the six enterotoxins analysed, none of the S. aureus harboured the genes sea, sec and seh. Only the seb, sei and sej were found respectively at rates of 2.75%, 3.67% and 26.6%. Final y, the gene for resistance to methicil in was found in 2.75% of the strains isolated. The analysis of the association between virulence genes and the presence of subclinical mastitis showed the importance of genes Spa lukD and SEJ. Antibiotic resistance testing revealed a good sensitivity of S. aureus to most of the antibiotics tested. This study showed the low virulence of the S aureus strains isolated. Keywords: Dairy cows - Subclinical mastitis - Staphylococcus aureus - PCR - Dakar Des enquetes menees au Senegal ont montre la forte correlation des staphylocoques aux mammites subcliniques chez les vaches laitieres. La presente etude avait pour but de caracteriser les souches de Staphylococcus aureus identifies dans les fermes laitieres a Dakar. Sur un total de 244 isolats de Staphylococcus spp isoles de 135 vaches laitieres a mammites subcliniques dans six troupeaux de vaches laitieres en zone peri-urbaine de Dakar, 109 souches de S. aureus ontete isolees et identifiees par des methodes phenotypiques. Par PCR, les genes de la thermonuclease et des antigenes capsulaires majeurs 5 et 8 ont ete identifies respectivement chez 98,17%, 93.58% et 1,84% des souches de S. aureus. Parmi les genes de virulence recherches, les genes spa, Luk D et Luk S ont ete retrouves respectivement chez 68,81%, 62,39% et 0,92% des souches de S. aureus. En revanche, les genes lukF, lukM et tst1 etaient absents. Parmi les six enterotoxines recherchees, sec et seh etaient absentes, seb, sei et sej ont ete retrouvees respectivement chez 2,75%, 3,67% et de 26,6% des souches. Enfin, le gene de resistance a la methicil ine a ete retrouve dans 2,75% des souches isolees. L’analyse de l’association entre les genes de virulence et la presence de mammites subcliniques a montre l’importance des genes spa, lukD et sej. Une bonne sensibilite de S. aureus a ete notee a la plupart des antibiotique","PeriodicalId":75643,"journal":{"name":"Bulletin of animal health and production in Africa. Bulletin des sante et production animales en Afrique","volume":"58 1","pages":"201-210"},"PeriodicalIF":0.0,"publicationDate":"2011-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70487382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-08DOI: 10.4314/BAHPA.V58I3.64209
A. O. Oguleye, A. T. Ajuwape, A. Adetosoye
The susceptibility of 41 Salmonella enterica isolates isolated from poultry disease outbreaks between April 2005 and August 2007 in Oyo and Ogun States Nigeria to ciprofloxacin, nalidixic acid, chloramphenicol, streptomycin, kanamycin, ampicil in, neomycin, and tetracycline was determined. Using microtitre method for MIC determination, 22 resistance patterns were obtained. These include an octuple resistant pattern manifested by six isolates; three different septuple resistance patterns exhibited by four isolates, eight isolates manifested three dif erent sextuple resistance patterns, six isolates exhibited five different quintuple resistant patterns, three dif erent quadruple resistant patterns were presented by six isolates, five dif erent triple resistance patterns were shown by eight isolates, whereas two isolates showed double resistant pattern and a single resistance to ampicillin was seen in one isolate. A total of 30/41 (73.2%) were multi-drug resistant, showing resistance to four or eight antibiotics. R-type cip.amp.str.chl.kan.neo.tet.nal was the commonest seen in six isolates. Of al the isolates 26/ 41(63.4%) were ciprofloxacin resistant. This investigation reveals the widespread occurrence of multidrug resistant Salmonel a serovars from poultry disease outbreaks in Nigeria. Key words: Fluoroquinolone resistant Salmonel a enterica, Poultry, Multidrug-resistant. On a determine la susceptibilite de 41 isolats de Salmonella enteric isoles des maladies des epidemies de la volail e entre avril 2005 et aout 2007 dans les Etats d’Oyo et d’Ogun du Nigeria a la ciprofloxacine, a l’acide naledixique, au chloramphenicol, au streptomycine, au kanamycine, a l’ampicil ine, au neomycine et au tetracycline. En utilisant la methode de microtitration pour la determination de la CMI (concentration minimale inhibitrice), 22 profils de resistance ont ete obtenus. Il s’agit notamment du modele resistant octuple manifeste par six isolats; trois differents profils de resistance septuple exposes par quatre isolats, huit isolats qui manifestent trois differents profils de resistance aux sextuples, six isolats presentaient cinq differents modes de resistance quintuple, trois differents modes de quadruple resistants ont ete presentes par six isolats, cinq differents profils de resistance aux triples ont ete montrees par huit isolats alors que deux isolats ont montre un double modele resistant et une resistance a l’ampicil ine uniquement a ete observee dans un isolat. Un total de 30/41 (73,2%) resistait aux medicaments multiples, presentant une resistance a quatre ou huit antibiotiques. Le type R cip.amp.str.chl.kan.neo.tet.nal etait plus frequemment vu dans six isolats. De tous les isolats de 26/41 (63,4%) etaient resistant a la ciprofloxacine. Cette enquete revele la presence generalisee de la Salmonel a de type serologique multiresistant dans les epidemies de maladies de la volail e au Nigeria.
测定了2005年4月至2007年8月在尼日利亚奥约州和奥贡州暴发的家禽疾病暴发中分离的41株肠沙门氏菌对环丙沙星、纳利地酸、氯霉素、链霉素、卡那霉素、阿霉素、新霉素和四环素的敏感性。采用微滴法测定MIC，得到22种耐药模式。其中包括六株菌株表现出的八元耐药模式;4株菌株表现出3种不同的七重耐药模式，8株菌株表现出3种不同的六重耐药模式，6株菌株表现出5种不同的五重耐药模式，6株菌株表现出3种不同的四重耐药模式，8株菌株表现出5种不同的三重耐药模式，2株菌株表现出双重耐药模式，1株菌株表现出对氨苄西林的单一耐药模式。多药耐药30/41(73.2%)，分别对4种或8种抗生素耐药。r型cip.amp.str.chl.kan.neo.tet.nal在6株分离株中最常见。其中26/ 41株(63.4%)对环丙沙星耐药。这项调查揭示了尼日利亚家禽疾病暴发中广泛发生的耐多药沙门氏菌a血清型。关键词:耐氟喹诺酮类肠沙门氏菌，家禽，多重耐药2005年4月至2007年前后，尼日利亚奥约州和奥贡州的41株肠源性沙门氏菌分离株被检测出对环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星、环丙沙星和环四环素的敏感性。采用微量滴定法测定最小抑制浓度(CMI)， 22个谱线对药物的抗药效果良好。模型抗药八元体在6个分离株中表现为抗药八元体;三种不同类型的电阻七组暴露出四分之一隔离，三种不同类型的电阻六组，六种不同类型的电阻五组，三种不同类型的电阻四组，三种不同类型的电阻四组，三种不同类型的电阻四组，三种不同类型的电阻四组，三种不同类型的电阻六组。五点不同资料de阻力辅助三元组安大略省的高频装饰音管par huit isolats两个变量那么isolats安大略省的装饰音管联合国抗双模型等一个电阻l 'ampicil快乐或者疾病observee在联合国isolat。共有30/41例(73.2%)耐药，其中1 /4耐药。Le type R cip.amp.str.chl.kan.neo.tet.nal etta1加频率vu为6个隔离器。这些分离株中有26/41(63.4%)对环丙沙星具有耐药性。在尼日利亚，沙门氏菌病和多耐药病毒的血清学型流行病学调查显示存在。
{"title":"Fluoroquinolone resistant Salmonella enterica of poultry origin from south western states of Nigeria.","authors":"A. O. Oguleye, A. T. Ajuwape, A. Adetosoye","doi":"10.4314/BAHPA.V58I3.64209","DOIUrl":"https://doi.org/10.4314/BAHPA.V58I3.64209","url":null,"abstract":"The susceptibility of 41 Salmonella enterica isolates isolated from poultry disease outbreaks between April 2005 and August 2007 in Oyo and Ogun States Nigeria to ciprofloxacin, nalidixic acid, chloramphenicol, streptomycin, kanamycin, ampicil in, neomycin, and tetracycline was determined. Using microtitre method for MIC determination, 22 resistance patterns were obtained. These include an octuple resistant pattern manifested by six isolates; three different septuple resistance patterns exhibited by four isolates, eight isolates manifested three dif erent sextuple resistance patterns, six isolates exhibited five different quintuple resistant patterns, three dif erent quadruple resistant patterns were presented by six isolates, five dif erent triple resistance patterns were shown by eight isolates, whereas two isolates showed double resistant pattern and a single resistance to ampicillin was seen in one isolate. A total of 30/41 (73.2%) were multi-drug resistant, showing resistance to four or eight antibiotics. R-type cip.amp.str.chl.kan.neo.tet.nal was the commonest seen in six isolates. Of al the isolates 26/ 41(63.4%) were ciprofloxacin resistant. This investigation reveals the widespread occurrence of multidrug resistant Salmonel a serovars from poultry disease outbreaks in Nigeria. Key words: Fluoroquinolone resistant Salmonel a enterica, Poultry, Multidrug-resistant. On a determine la susceptibilite de 41 isolats de Salmonella enteric isoles des maladies des epidemies de la volail e entre avril 2005 et aout 2007 dans les Etats d’Oyo et d’Ogun du Nigeria a la ciprofloxacine, a l’acide naledixique, au chloramphenicol, au streptomycine, au kanamycine, a l’ampicil ine, au neomycine et au tetracycline. En utilisant la methode de microtitration pour la determination de la CMI (concentration minimale inhibitrice), 22 profils de resistance ont ete obtenus. Il s’agit notamment du modele resistant octuple manifeste par six isolats; trois differents profils de resistance septuple exposes par quatre isolats, huit isolats qui manifestent trois differents profils de resistance aux sextuples, six isolats presentaient cinq differents modes de resistance quintuple, trois differents modes de quadruple resistants ont ete presentes par six isolats, cinq differents profils de resistance aux triples ont ete montrees par huit isolats alors que deux isolats ont montre un double modele resistant et une resistance a l’ampicil ine uniquement a ete observee dans un isolat. Un total de 30/41 (73,2%) resistait aux medicaments multiples, presentant une resistance a quatre ou huit antibiotiques. Le type R cip.amp.str.chl.kan.neo.tet.nal etait plus frequemment vu dans six isolats. De tous les isolats de 26/41 (63,4%) etaient resistant a la ciprofloxacine. Cette enquete revele la presence generalisee de la Salmonel a de type serologique multiresistant dans les epidemies de maladies de la volail e au Nigeria.","PeriodicalId":75643,"journal":{"name":"Bulletin of animal health and production in Africa. Bulletin des sante et production animales en Afrique","volume":"58 1","pages":"218-224"},"PeriodicalIF":0.0,"publicationDate":"2011-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70487436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-08DOI: 10.4314/BAHPA.V58I3.64218
F. G. Sodeinde, A. A. Akingbade, S. Amao, T. Rafiu, J. A. Alalade
The effect of legume intercrop on regrowth ability and nutritive value via in vitro gas production of guinea grass was evaluated in a 2 x 2 factorial arrangement. Panicum maximum cv T 58 grass with Canavalia ensiformis intercrop was studied for six weeks. The tiller height, tiller numbers, regrowth percentage were recorded on weekly basis. The sward was harvested at the end of six weeks. Samples were taken, oven dried and dry matter yield determined. Proximate composition of the grasses was then carried out. The soil condition was improved after treatment. The stem had the least crude protein content than the leaves (5.43%). In vitro gas production carried out showed that methane gas production was higher in the stem fraction than in the leaves. This suggests that leave utilization will be higher when fed to ruminants. Intercropping Canavalia ensiformis with Panicum maximum enhanced the regrowth ability and in vitro gas production thereby reducing the necessity for chemical fertilizer. Keywords: Canavalia ensiformis , Regrowth ability, In vitro gas, Panicum maximum L’effet de la culture intercalaire de legumineuses sur la capacite de regeneration et sur la valeur nutritive a travers la production de gaz in-vitro de l’herbe de Guinee a ete evalue dans un arrangement factoriel de 2 x 2. L’herbe Panicum maximum cv T 58 et la culture intercalaire de Canavalia ensiformis ont ete etudie pendant six semaines. La hauteur de la taille, le nombre de tailles, le pourcentage de repousse ont ete enregistre hebdomadairement. L’herbe a ete fauchee a la fin de la sixieme semaines. Des echantillons ont ete preleves et seches au four. La matiere seche produite a ete analysee. La composition generale des graminees a ensuite ete effectuee. L’etat du sol a ete ameliore apres le traitement. Le pedicelle avait une plus faible quantite de proteines brutes que les feuilles (5,43%). La production de gaz effectuee in vitro a demontre que le gaz methane produit etait plus eleve dans le pedicelle que dans les feuilles. Cela suggere que l’utilisation des feuilles serait plus importante quand elles servent de nourriture pour les ruminants. La culture intercalaire de Canavalia ensiformis couplee au Panicum maximum a renforce la capacite de repousse et la production in vitro de gaz reduisant ainsi le besoin de recourir aux engrais chimiques.
采用2 × 2因子法评价豆科间作对豚草再生能力和体外产气营养价值的影响。间作菜豆草的大穗草cv t58草进行了为期6周的试验研究。每周记录分蘖高度、分蘖数、再生率。六周后，草被收割了。取样品，烘箱烘干，测定干物质产率。然后进行了草的近似组成。处理后土壤状况得到改善。茎粗蛋白质含量最低，为叶片的5.43%。体外产气试验表明，茎段的甲烷产气量高于叶片。这说明反刍动物对叶片的利用率更高。苜蓿与大头草间作可提高植株的再生能力和体外产气量，从而减少对化肥的需要量。关键词:大黄芥;再生能力;离体气体;大黄芥(Panicum maximum);豆科植物;再生能力;L 'herbe Panicum maximum cv t58 et la culture intercalaire de Canavalia ensiformis研究了6个位点。在登记登记前，我们将为您提供高级服务、高级服务、高级服务。我想我是一个很好的朋友，我是一个很好的朋友。这两种机制都是在四种情况下发生的。这就产生了一个完整的分析。La composition generale des graminees a ensuite ete effecee。我的灵魂是一个伟大的灵魂，我的灵魂是一个伟大的灵魂。Le pedicelle ava1 + fail定量de proteinbrutes que les feuilles(5.43%)。在离体条件下，产气效果良好，产气量大，产气量大，产气量大，产气量大，产气量大，产气量大，产气量大，产气量大。Cela建议对食物的利用和对反刍动物的营养服务进行重要的利用。在体外培养中，加那伐利亚(canavaliaensiformis)和帕尼伐利亚(Panicum)最大限度地增强了酶解能力，并在体外产生了抗氧化酶。
{"title":"Intercroping effect of Canavalia Ensiformis on regrowth ability and in-vitro gas production of Panicum Maximum Cvt58.","authors":"F. G. Sodeinde, A. A. Akingbade, S. Amao, T. Rafiu, J. A. Alalade","doi":"10.4314/BAHPA.V58I3.64218","DOIUrl":"https://doi.org/10.4314/BAHPA.V58I3.64218","url":null,"abstract":"The effect of legume intercrop on regrowth ability and nutritive value via in vitro gas production of guinea grass was evaluated in a 2 x 2 factorial arrangement. Panicum maximum cv T 58 grass with Canavalia ensiformis intercrop was studied for six weeks. The tiller height, tiller numbers, regrowth percentage were recorded on weekly basis. The sward was harvested at the end of six weeks. Samples were taken, oven dried and dry matter yield determined. Proximate composition of the grasses was then carried out. The soil condition was improved after treatment. The stem had the least crude protein content than the leaves (5.43%). In vitro gas production carried out showed that methane gas production was higher in the stem fraction than in the leaves. This suggests that leave utilization will be higher when fed to ruminants. Intercropping Canavalia ensiformis with Panicum maximum enhanced the regrowth ability and in vitro gas production thereby reducing the necessity for chemical fertilizer. Keywords: Canavalia ensiformis , Regrowth ability, In vitro gas, Panicum maximum L’effet de la culture intercalaire de legumineuses sur la capacite de regeneration et sur la valeur nutritive a travers la production de gaz in-vitro de l’herbe de Guinee a ete evalue dans un arrangement factoriel de 2 x 2. L’herbe Panicum maximum cv T 58 et la culture intercalaire de Canavalia ensiformis ont ete etudie pendant six semaines. La hauteur de la taille, le nombre de tailles, le pourcentage de repousse ont ete enregistre hebdomadairement. L’herbe a ete fauchee a la fin de la sixieme semaines. Des echantillons ont ete preleves et seches au four. La matiere seche produite a ete analysee. La composition generale des graminees a ensuite ete effectuee. L’etat du sol a ete ameliore apres le traitement. Le pedicelle avait une plus faible quantite de proteines brutes que les feuilles (5,43%). La production de gaz effectuee in vitro a demontre que le gaz methane produit etait plus eleve dans le pedicelle que dans les feuilles. Cela suggere que l’utilisation des feuilles serait plus importante quand elles servent de nourriture pour les ruminants. La culture intercalaire de Canavalia ensiformis couplee au Panicum maximum a renforce la capacite de repousse et la production in vitro de gaz reduisant ainsi le besoin de recourir aux engrais chimiques.","PeriodicalId":75643,"journal":{"name":"Bulletin of animal health and production in Africa. Bulletin des sante et production animales en Afrique","volume":"58 1","pages":"280-285"},"PeriodicalIF":0.0,"publicationDate":"2011-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70487609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-08DOI: 10.4314/BAHPA.V58I4.64238
J. Kibugu, M. W. Muchiri, N. Mbugua, J. N. Mwangi, J. Thuita
{"title":"Short Communication: Comparative Evaluation of Anticoagulatory Activity of Ethylenediamine Tetra-Acetic Acid (Edta) and Heparin For Haematological Analysis.","authors":"J. Kibugu, M. W. Muchiri, N. Mbugua, J. N. Mwangi, J. Thuita","doi":"10.4314/BAHPA.V58I4.64238","DOIUrl":"https://doi.org/10.4314/BAHPA.V58I4.64238","url":null,"abstract":"","PeriodicalId":75643,"journal":{"name":"Bulletin of animal health and production in Africa. Bulletin des sante et production animales en Afrique","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70487698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-08DOI: 10.4314/BAHPA.V58I4.64233
J. Kana, A. Téguia, J. T. Choumboue
One hundred and twenty 3-week old male broiler chickens were used to evaluate the effects of dietary charcoal from Canarium schweinfurthii (charcoal A) and maize cob (charcoal B) on aflatoxin B1 toxicosis in broiler chickens. The individually caged birds were randomly allotted to 8 groups of 15 birds and fed in a completely randomised design a diet with either fresh groundnut meal (C-), groundnut meal infested with 22.02 ppb of aflatoxin B1 (C+) or diet C+ supplemented with either 0.2 or 0.4% of charcoal A (A0.2 and A0.4 respectively), charcoal B (B0.2 and B0.4 respectively) or a 1/1 mixture of A and B (M0.2 and M0.4 respectively). The results indicated that the inclusion of charcoal either individually or as a mixture significantly (P 0.05) by the treatments. Both charcoal A and B significantly (P Key words: Aflatoxin B1, Broiler chickens, Canarium schweinfurthii, Maize cob, Plant charcoal, Toxin biner. Cent vingt poussins males âges de 3 semaines eleves en cage dans un dispositif completement aleatoire ont ete utilises pour evaluer l’effet du charbon des noyaux de Canarium schweinfurthii (charbon A), de rafles de mais (charbon B) ou de leur association (M) dans la proportion 1/1 comme capteur d’aflatoxine B1 dans l’aliment de poulet de chair. A cet effet, les poussins ont recu soit un aliment contenant du tourteau d’arachide de bonne qualite (C-) soit un aliment (C+) contenant du tourteau d’arachide infeste d’Aspergillus flavus (22,02ppb/kg d’aflatoxine B1), soit l’aliment C+ supplemente avec 0,2 ou 0,4% de charbon active de noyaux de Canarium (A0,2 et A0,4), de rafles de mais (B0,2 et B0,4) ou de l’association des charbons A et B (M0,2 et M0,4). La consommation alimentaire a ete significativement (P Mots cles: Aflatoxine B1, Canarium schweinfurthii, Capteur de toxine, Charbon vegetaux, Poulets de chair, Rafles de mais.
以123周龄肉鸡为试验对象，研究了饲粮中添加蓖麻炭(charcoal A)和玉米芯炭(charcoal B)对肉鸡黄曲霉毒素B1中毒的影响。将单笼饲养的雏鸟随机分为8组，每组15只，采用完全随机设计，分别饲喂新鲜花生粕(C-)、含22.02 ppb黄曲霉毒素B1 (C+)的花生粕或在饲料C+中添加0.2或0.4%的木炭a (A0.2和A0.4)、木炭B (B0.2和B0.4)或a和B的1/1混合物(M0.2和M0.4)。结果表明，不同处理对炭的单独或混合包埋均有显著影响(P < 0.05)。关键词:黄曲霉毒素B1，肉仔鸡，香菜，玉米芯，植物炭，毒素剂。1/1捕集黄曲霉素B1和1/1捕集黄曲霉素B1和1/1捕集黄曲霉素B1和1捕集黄曲霉素B1和1捕集黄曲霉素。一个cet(中央东部东京),里面的普桑recu所以联合国滋养品contenant du tourteau d 'arachide好的质量(C -)所以联合国食物(C +) contenant du tourteau d 'arachide infeste d 'Aspergillus flavus ppb /公斤(22日02 d 'aflatoxine B1),所以l 'aliment C + supplemente用0,2或者0,4%炭疽活跃de noyaux de Canarium (A0, 2等A0, 4),德rafles de但是(B0, 2 B0, 4)或者de l 'association des炭疽等B (M0, 2等M0, 4)。植物:黄曲霉毒素B1，香菜，毒素捕获器，碳蔬菜，椅子Poulets de chair，莱弗斯。
{"title":"The evaluation of activated dietary charcoal from Canarium schweinfurthii Engl. seed and maize cob as toxin binder in broiler chickens.","authors":"J. Kana, A. Téguia, J. T. Choumboue","doi":"10.4314/BAHPA.V58I4.64233","DOIUrl":"https://doi.org/10.4314/BAHPA.V58I4.64233","url":null,"abstract":"One hundred and twenty 3-week old male broiler chickens were used to evaluate the effects of dietary charcoal from Canarium schweinfurthii (charcoal A) and maize cob (charcoal B) on aflatoxin B1 toxicosis in broiler chickens. The individually caged birds were randomly allotted to 8 groups of 15 birds and fed in a completely randomised design a diet with either fresh groundnut meal (C-), groundnut meal infested with 22.02 ppb of aflatoxin B1 (C+) or diet C+ supplemented with either 0.2 or 0.4% of charcoal A (A0.2 and A0.4 respectively), charcoal B (B0.2 and B0.4 respectively) or a 1/1 mixture of A and B (M0.2 and M0.4 respectively). The results indicated that the inclusion of charcoal either individually or as a mixture significantly (P 0.05) by the treatments. Both charcoal A and B significantly (P Key words: Aflatoxin B1, Broiler chickens, Canarium schweinfurthii, Maize cob, Plant charcoal, Toxin biner. Cent vingt poussins males âges de 3 semaines eleves en cage dans un dispositif completement aleatoire ont ete utilises pour evaluer l’effet du charbon des noyaux de Canarium schweinfurthii (charbon A), de rafles de mais (charbon B) ou de leur association (M) dans la proportion 1/1 comme capteur d’aflatoxine B1 dans l’aliment de poulet de chair. A cet effet, les poussins ont recu soit un aliment contenant du tourteau d’arachide de bonne qualite (C-) soit un aliment (C+) contenant du tourteau d’arachide infeste d’Aspergillus flavus (22,02ppb/kg d’aflatoxine B1), soit l’aliment C+ supplemente avec 0,2 ou 0,4% de charbon active de noyaux de Canarium (A0,2 et A0,4), de rafles de mais (B0,2 et B0,4) ou de l’association des charbons A et B (M0,2 et M0,4). La consommation alimentaire a ete significativement (P Mots cles: Aflatoxine B1, Canarium schweinfurthii, Capteur de toxine, Charbon vegetaux, Poulets de chair, Rafles de mais.","PeriodicalId":75643,"journal":{"name":"Bulletin of animal health and production in Africa. Bulletin des sante et production animales en Afrique","volume":"58 1","pages":"375-381"},"PeriodicalIF":0.0,"publicationDate":"2011-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70488051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-08DOI: 10.4314/BAHPA.V58I3.64220
B. Emikpe, O. Ohore, O. Oladele, B. A. Saka
{"title":"Short Communication: Influence of Levamisole on antibody response to Newcastle disease vaccination in chemically Immunosupressed Broiler Chicken.","authors":"B. Emikpe, O. Ohore, O. Oladele, B. A. Saka","doi":"10.4314/BAHPA.V58I3.64220","DOIUrl":"https://doi.org/10.4314/BAHPA.V58I3.64220","url":null,"abstract":"","PeriodicalId":75643,"journal":{"name":"Bulletin of animal health and production in Africa. Bulletin des sante et production animales en Afrique","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70487359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-03-08DOI: 10.4314/BAHPA.V58I3.64221
W. Tigre, T. Gudeta, F. Regassa
{"title":"Short Communication: Preliminary study on Bovine Tuberculosis in Nekemte Municipailty Abattoir, WesternEthiopia.","authors":"W. Tigre, T. Gudeta, F. Regassa","doi":"10.4314/BAHPA.V58I3.64221","DOIUrl":"https://doi.org/10.4314/BAHPA.V58I3.64221","url":null,"abstract":"","PeriodicalId":75643,"journal":{"name":"Bulletin of animal health and production in Africa. Bulletin des sante et production animales en Afrique","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70487416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：