Current topics in molecular endocrinology最新文献

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Androgen binding in the testis: in vitro production of androgen binding protein (ABP) by Sertoli cell cultures and measurement of nuclear bound androgen by a nuclear exchange assay. 睾丸中的雄激素结合:通过支持细胞培养体外产生雄激素结合蛋白(ABP)和通过核交换试验测量核结合雄激素。

Current topics in molecular endocrinology

Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_21

B M Sanborn, J S Elkington, A Steinberger, E Steinberger

Androgen binding activity in the testis has two components. One component, ABP, has been shown to be produced by Sertoli cell cultures for at least 9 days in the absence of exogenously added hormones. FSH (10-100 microgram/ml) markedly enhances the secretion of ABP. MIX has a potentiating effect after long treatment intervals (7 days). In order to study the second component, intracellular androgen receptor, a nuclear exchange assay was developed. Competition for exchange activity using 3H-dihydrotestosterone was significant for a 500 fold excess of testosterone, dihydrotestosteron, progesterone, and cyproterone acetate. The exchange activity was increased 2-10 fold by prior treatment in vitro or in vivo with testosterone. Significant exchange activity was found in long-term hypophysectomized adult and immature animals and in tubule and germ cell fractions. In isolated germ cell fractions, the highest concentration of exchange activity was associated with the most mature elements. These data suggest that androgen exchange activity may exist in both Sertoli cell and germ cell fractions and suggest that the mechanism of action of androgens in the testis is quite complex.

睾丸中的雄激素结合活性有两个组成部分。其中一种成分ABP已被证明在没有外源添加激素的情况下，由支持细胞培养至少9天产生。FSH(10-100微克/毫升)显著促进ABP的分泌，MIX在较长治疗间隔(7天)后具有增强作用。为了研究第二组分，细胞内雄激素受体，开发了核交换试验。使用3h -二氢睾酮对交换活性的竞争对于睾酮、二氢睾酮、孕酮和醋酸环丙孕酮的500倍过量是显著的。通过体外或体内睾酮治疗，交换活性增加2-10倍。在长期去垂体的成年和未成熟动物以及小管和生殖细胞中发现了显著的交换活性。在分离的生殖细胞组分中，交换活性浓度最高的是最成熟的元素。这些数据表明雄激素交换活性可能存在于睾丸支持细胞和生殖细胞中，并提示雄激素在睾丸中的作用机制相当复杂。

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引用次数: 39

Transcription during mammalian spermatogenesis with special reference to Sertoli cells. 哺乳动物精子发生过程中的转录，特别涉及支持细胞。

Current topics in molecular endocrinology

Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_32

L L Tres, A L Kierszenbaum

引用次数: 4

Gonadotropic regulation of 5 alpha-reductase activity in the interstitial cells and whole testis homogenate of the immature rat. 促性腺激素对未成熟大鼠间质细胞和全睾丸匀浆中5 - α还原酶活性的调节。

Current topics in molecular endocrinology

Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_4

S N Nayfeh, J C Coffey, N J Kotite, F S French

引用次数: 10

Synthesis of estradiol-17 beta by Sertoli cells in culture: stimulation by FSH and dibutyryl cyclic AMP. 培养的支持细胞合成雌二醇-17 β: FSH和二丁基环AMP的刺激。

Current topics in molecular endocrinology

Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_6

D T Armstrong, Y S Moon, I B Fritz, J H Dorrington

Sertoli cells isolated from the testes of 18 to 20-day old rats synthesize estradiol-17 beta when grown in primary culture in the presence of testosterone (5 X 10(-7) M). After an initial lag phase of about 2 hours, follicle-stimulating hormone (FSH) stimulates synthesis of estradiol up to 50-fold, synthesis being approximately linear for 24 hours when medium is changed every 2-6 hours. Luteinizing hormone (LH) causes only a marginal stimulation, at concentrations consistent with contamination with FSH. Dibutyryl cyclic 3',5'-adenosine monophosphate (dbcAMP) (10(-4)M) mimicks the effect of FSH. No significant synthesis occurs in the absence of steroid substrate or in the presence of pregnenolone (5 X 10(-7) M). When cells are pre-incubated for 24 hours with FSH in the absence of testosterone, addition of testosterone results in an immediated increase in estradiol synthesis without a lag phase, suggesting that FSH or dbcAMP are capable of inducing the conditions necessary for stimulation in the absence of testosterone. These observations provide the first direct evidence of estradiol-17 beta synthesis by Sertoli cells from normal animals, and offer evidence that synthesis of this steroid is regulated at the level of the aromatizing enzyme system by FSH and cyclic AMP.

从18 - 20日龄大鼠睾丸中分离出的支持细胞，在存在睾酮(5 × 10(-7) M)的原代培养中生长时，合成雌二醇-17 β。经过大约2小时的初始滞后期，卵泡刺激素(FSH)刺激雌二醇的合成高达50倍，当每2-6小时更换一次培养基时，合成在24小时内近似线性。促黄体生成素(LH)在浓度与卵泡刺激素污染一致时，只会引起轻微的刺激。二丁基环3′，5′-腺苷单磷酸(dbcAMP) (10(-4)M)模拟FSH的作用。在没有类固醇底物或孕烯醇酮存在的情况下，没有明显的合成(5 × 10(-7) M)。当细胞在没有睾酮的情况下与FSH预孵育24小时，添加睾酮会导致雌二醇合成立即增加，而没有滞后期，这表明FSH或dbcAMP能够在没有睾酮的情况下诱导刺激所需的条件。这些观察结果为正常动物的Sertoli细胞合成雌二醇-17 β提供了第一个直接证据，并提供了证据，证明这种类固醇的合成是由FSH和环AMP在芳香化酶系统水平上调节的。

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引用次数: 19

Physicochemical and biological properties of androgen receptors. 雄激素受体的物理化学和生物学特性。

Current topics in molecular endocrinology

Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_17

C W Bardin, O Jänne, L P Bullock, S T Jacob

引用次数: 8

Androgen transport and receptor mechnisms in testis and epididymis. 睾丸和附睾雄激素转运和受体机制。

Current topics in molecular endocrinology

Pub Date : 1974-01-01 DOI: 10.1007/978-1-4684-2595-6_16

F S French, W S McLean, A A Smith, D J Tindall, S C Weddington, P Petrusz, M Sar, W E Stumpf, S N Nayfeh

引用次数: 4

Conformational and metabolic aspects of gonadotropins. 促性腺激素的构象和代谢方面。

Current topics in molecular endocrinology

Pub Date : 1974-01-01 DOI: 10.1007/978-1-4684-2595-6_6

D Puett, M Ascoli, L A Holladay

引用次数: 11

Testicular estradiol receptors in the rat. 大鼠睾丸雌二醇受体。

Current topics in molecular endocrinology

Pub Date : 1974-01-01 DOI: 10.1007/978-1-4684-2595-6_21

E Mulder, W M van Beurden-Lamers, W De Boer, A O Brinkman, H J van der Molen

Specific high affinity saturable binding proteins for oestradiol-17beta have been demonstrated in the cytoplasm of liver, adrenal, pituitary, prostate, epididymis and testis interstitial tissue of the rat. Specific testicular oestradiol receptors were present in cytoplasmic and nuclear fractions of interstitial tissue, but not in seminiferous tubules of rat testis. After in vivo or in vitro administration of estradiol the steroid was bound by the nuclear fraction of interstitial tissue only in the presence of the cytoplasmic receptor. Using agar gel electrophoresis the cytoplasmic oestradiol receptor from rat testis could be separated from the testicular androgen binding protein (ABP) and from a specific androgen receptor.

在大鼠肝脏、肾上腺、垂体、前列腺、附睾和睾丸间质组织的细胞质中发现了特异性高亲和力的雌二醇-17 β饱和结合蛋白。特异性睾丸雌二醇受体存在于大鼠睾丸间质组织的细胞质和细胞核部分，而不存在于精小管中。体内或体外给予雌二醇后，类固醇仅在细胞质受体存在的情况下才与间质组织的核部分结合。利用琼脂凝胶电泳技术，可以将大鼠睾丸细胞质雌二醇受体与睾丸雄激素结合蛋白(ABP)及特异性雄激素受体分离。

引用次数: 21

Effects of FSH on levels of androgen binding protein in the testis. 卵泡刺激素对睾丸雄激素结合蛋白水平的影响。

Current topics in molecular endocrinology

Pub Date : 1974-01-01 DOI: 10.1007/978-1-4684-2595-6_19

I B Fritz, B Kopec, K Lam, R G Vernon

引用次数: 42

FSH binding in rat testes during maturation and following hypophysectomy. Cellular localization of FSH receptors. 成熟和垂体切除术后大鼠睾丸中FSH的结合。FSH受体的细胞定位。

Current topics in molecular endocrinology

Pub Date : 1974-01-01 DOI: 10.1007/978-1-4684-2595-6_10

A Steinberger, K H Thanki, B Siegal

引用次数: 52

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