Folia histochemica et cytochemica最新文献

The effect of x-ray irradiation with doses of 0.5 Gy and 3 Gy on the number of the spermatozoa and the structure of seminiferous tubules in the inbred strains of mice KE, KP, CBA/Kw and C57/Kw was investigated on the 28th day following irradiation. Differences in the radiosensitivity between the studied strains were found. C57/Kw males revealed the highest resistance to x-rays as manifested by the absence of significant differences in the number of the spermatozoa and the relative frequency of tubules at stages VII-XII of the spermatogenetic cycle between control animals and those irradiated with 0.5 Gy. The same dose caused a decrease in the number of spermatozoa and altered the frequency of the particular stages in the other strains. Following irradiation with 3 Gy the number of spermatozoa in the C57/Kw strains declined but still was significantly higher than in the other strains. The latter dose brought about an inhibition of the spermatogenesis in all the studied strains to the extent of degeneration of the seminiferous tubules which contained no more than occasional spermatogonia. The percentage of such degenerated tubules in the C57/Kw strain was, however, as low as 14.5%, whereas it approximated 50% in the KE and KP strain.

The localization of sulfomucins and sialomucins present in the various types of mucus cells--secretory (SMC) and mature non-secretory mucus cells (MMC) of the different regions of the alimentary canal in Labeo rohita (Ham.) has been studied by fluorescence microscopical techniques (viz., Ferric alum Coriphosphine--FAC, Coriphosphine thiazol yellow--CTY and Dische reaction--DR). High degree of fluorescence activity confirms the presence of relatively high amount of sulfomucins in the mucus cells of the oesophageal and buccopharyngeal regions when tested with FAC and CTY methods while moderate intensity of the same in the mucus cells of the two aforesaid regions indicates the localization of relatively lesser amount of sialomucins studied by DR technique. Moderate degree of fluorescence is also observed in the mucus cells (in both SMC and MMC) of the intestinal region with the techniques of FAC, CTY and DR whereas weak fluorescence of FAC, CTY and DR methods is marked in the mucus cells (in both SMC and MMC) of the rectal region due to the presence of low amount of sulfomucins and sialomucins.

Activity of acid phosphatase, beta-glucuronidase and N-acetyl-beta-D-glucosaminidase in peripheral blood neutrophils of 30 women with breast cancer has been studied by means of semiquantitative cytochemical methods. In comparison to 20 healthy women the patients showed an increased activity of acid phosphatase and beta-glucuronidase whereas that of N-acetyl-beta-D-glucosaminidase was significantly lowered. It has been suggested that observed intracellular enzymatic deficiency is of importance with regard to lowered antitumor activity of neutrophils. Analogous deficiency of N-acetyl-beta-D-glucosaminidase within the neutrophils has previously been observed by the authors in women with malignant tumors of reproductive organs.

The adrenal structure was studied in three inbred mice strains: KP, KE, and CBA. In adult females the adrenal weight and size was higher than in males from the respective strains. It resulted from the higher adrenocortical volume in females, from the higher volume of particular adrenocortical zones and from the presence of an undegenerated x-zone. In the KE females the x-zone deserves a particular attention because of its unusual morphology, different from that observed in females from the other strains. Significant differences were found between the investigated strains in the volume of particular adrenocortical zones, as well as in the number and size of the adrenocortical cells. The above differences were confirmed by the morphometric and sterereologic analysis using Weibel's method.

The fossil dinosaur bones over 80 million years old were studied. Samples for examination were prepared with specially elaborated methods. Observations in the light and scanning electron microscope yielded images of the spatial distribution of vascular canals. Besides, the contents of these canals were isolated; the contents fully corresponded to the vascular network in dinosaur bones under study, this permitted the morphological assessment of the vascular system. In the dinosaur bone, independently of its type, i.e. whether it was primitive e.g. plexiform, or Haversian , two blood inflow tracts were distinguished: the main, primary tract running from the marrow cavity, and the secondary tract departing from the periosteum. Examinations made with the use of an X-ray microanalyzer corroborated the essential importance of the tract running from the marrow cavity, revealing the presence of the same material (iron) in the wall of the marrow cavity and in the vascular canals closely connected with this part of the cavity. The bone was found to contain the axial vascular canals, whose course was parallel to the long axis of the bone, and the anastomosing canals perpendicular to them and thereby to the long axis of the bone. The system of the interlinked canals of the two types bounded the bone mass into a prism. The osseous space restricted by these canals was defined as the functional bone area, undoubtedly functionally subject to the control of the surrounding vessels. The spatial system of the functional bone areas was more orderly and these areas were markedly smaller in the primitive, plexiform bone than in the Haversian bone.(ABSTRACT TRUNCATED AT 250 WORDS)

The experiments were carried out on "Swiss" mice which received injections of mercuric chloride. The organs examined included the spleen and bone marrow. The detection of mercury in the cells of the above organs was performed according to the histochemical method developed by Timm[13]. In both cases Timm's Ioethod failed, proving to be invalid for determination of the lowest mercuric concentrations. By use of the hXE technique, it was found that the minimum concentration of mercury in the tissue detectable by cyto-chemical methods amounts to 0.65-1.0 ppm.

Brains and nerve cords of Spodoptera litura (Fabr.) of various stages during metamorphosis were tested for acid mycopolisaccharides (AMPS), PAS-positive substances, glycogen, proteins and lipids. During reorganisation of the neural lamella in pupal period glycogen and PAS-positive substances are stored in perineurium and AMPS in the glial lacunar system (GIS) and below the perineurium as a thin layer. Lipids and AMPS diffuse in the GIS. The perineurium and GIS serve for the passage as well as storage of nutrients. In neuropile, only proteins are present. Significances of these findings is discussed.

The purification of four different peptides from the NSP fraction of bovine plasma, using: organic solvents extraction, column Sephadex G-50 filtration, thin-layer Sephadex G-25 electrophoresis in 5 M guanidine hydrochloride and thin-layer Bio-Gel P-6 electrophoresis methods was described. The molecular weights of the isolated peptides and their amino acid composition were determined. The dose-dependent effect two of the four peptides on 3H-thymidine incorporation into BHK-21 and BHK/RSV cells was described. The results support previous observations that the NSP fraction contains a peptide growth prometing factor and a potent peptide growth inhibitor. The possible physiological role of the peptide growth inhibitor was discussed.

Slices of Morris hepatoma 7777 or rat liver isolated from control or turpentine-injected rats were incubated for 2 h with 14C-leucine. Radioactivities incorporated into albumin, alpha-fetoprotein, fibrinogen, alpha 1-AP-globulin, haptoglobin and alpha 1-acid glycoprotein were determined after the proteins had been isolated from the incubation medium or tissue homogenate by immunoprecipitation with monospecific antisera. It was found that hepatoma synthesizes fibrinogen, alpha 1-AP-globulin and alpha 1-acid glycoprotein in the amounts comparable to rat liver, whereas formation of albumin and haptoglobin is reduced 5- to 10-fold. Local inflammation elicited by injection of turpentine to tissue donors increased formation of acute-phase protein in liver slices but had no effect on synthesis of these proteins in preparations of Morris hepatoma, although certain ultrastructural changes in the Golgi complex were observed not only in the liver but also in the tumour.

The effect of gonadotropic hormones on androgen level in media of cultured Leydig cells isolated from mouse testes of various age was investigated. Changes of androgen level were connected with the age of mice. The highest secretory level of androgen was found in cultures of Leydig cells from mature 60 day old animals, which then decreased in 7 month old mice. Stimulating effect of LH and PRL on hormonal secretion by cultured Leydig cells was observed.