Folia histochemica et cytochemica最新文献

The author has investigated the carotenoids in the eggs utilized in form of caviar of 4 species of fishes. By means of columnar and thin-layer chromatography, the following carotenoids were found to be present: beta-carotene, beta-cryptoxanthin, echinenone, canthaxanthin, lutein, tunaxanthin, isozeaxanthin, zeaxanthin, salmoxanthin, adobixanthin, adonixanthin ester, astaxanthin and astaxanthin ester. The total carotenoid varied from 0.229 (Th. chlacogramma) to 1.669 microgram/g fresh weight (O. nerka).

The effect of Ethylmercury-p-toluenesulphanilide on the neurosecretory function of the hypothalamic-hypophyseal system has been studied. The p-toluenesulphanilide of ethylmercury is a pesticide with fungicidal properties. The poison has been administered intragastrically for 10 days at a daily dose of 4 mg. The results of experiments presented herewith have shown that ingestion of EMTSA lead to an accumulation of neurosecretory material in the nerve cells of the supraoptic and paraventricular nuclei as well as in those of the neurohypophysis. In spite of the increased content of neurosecretory substances in the hypothalamic-hypophyseal system, the secretory cells of the supraoptic and paraventricular nuclei did not show any features of enhanced secretory activity. Basing on the observations made in this study an assumption was raised that the intragastric poisoning by EMTSA exerted an inhibitory effect on the release of ADH from the neurosecretory system. Intoxication by EMTSA induced only negligible degenerative changes in neurocytes of the hypothalamic nuclei.

A method has been developed for the demonstration of neutral and acidic glycoproteins in and for preparing autoradiographs of rat nasal glands embedded in JB-4 embedding kit.

A comparative histochemical study of polysaccharides in some arteries of the region of the heart of black vulture was performed. With some exceptions, no variation in polysaccharides composition in all arteries studied were shown. Arterial walls present a sialo-mucopolysaccharidic complex and it is composed of: sialic acid, neutral mucopolysaccharides, hyaluronic acid and chondroitin sulfates. The concentration of these carbohydrates, mainly of the acidic ones, decrease from the inner portions of the media toward the adventitia.

On the basis of the light and electron microscopic studies of synchronously dividing cells of antheridial filaments in Chara vulgaris, it has been determined that mitochondria maintain their individuality throughout the whole cell cycle. They are mostly round or ovale in profiles, rarely rod-, and occasionally dumbbell-shaped. Synchronisation of mitochondrial cycles does not occur within a single cell: the increase in the number of mitochondria is gradual and continuous, and their average sizes are approximately constant. In all phases of the cell cycle mitochondria occupy about 1.3% of the cell volume. As in the case of the cell volume, neither the total volume nor the number of mitochondria duplicates in the course of interphase, both values reaching merely 2/3 of than characteristic of the previous cycle. Within a single filament, after 4 successive mitotic cycles leading to an 8-fold increase in number of haploid nuclei, the number of mitochondria is only 3 times higher. This phenomenon is connected with morphogenetic reduction of cellular dimensions in successive and gradually shorter mitotic cycles preceding the stage of spermatids. It is supposed that the shortening of the nuclear cycle in consecutive generations of antheridial filaments is not accompanied by a proportional reduction of the mitochondrial cycles.

The simultaneous influence of swim and thermal stress on catecholamine (CA) content in rat adrenal medulla was investigated histofluorimetrically in satiated and 24 h fasted rats. It was found that the increase of adrenal CA synthesis rate occurs in both fasted and satiated rats in state of hypothermia after the swimming at 20 degree C and in the hyperthermia caused by swimming at 47 degree. Similar release-induced increase of adrenal CA synthesis rate was observed after the swimming performed at 25 degree C only in fasted, but not in satiated rats. This increase was associated with the better physical condition and the less marked hypothermia in fasted animals compared to the satiated ones. The ambient temperature of 4 degree C causing the extreme hypothermia in both groups of animals failed to cause the CA synthesis acceleration, evoking the evident CA exhaustion in adrenals. The possibility of hypothermia-induced impairment of acetylcholinergic regulation of CA synthesis in adrenals--have been suggested. The different results concerning the body temperature, physical condition and the adrenal CA level obtained in fasted and satiated rats exposed to swim-stress at 25 degree C and 37 degree C have been discussed on the basis of the possible influence of metabolism rate on the adrenergic reaction to stress.

The effects of growing of Sertoli cells isolated from rat seminiferous epithelium by a modified procedure, and responses of these cells to dBcAMP or FSH stimulation was estimated using morphological methods. The modified isolation procedure included repeated mechanical rinsing of tubule pieces with a modified EDTA containing Hanks medium. Moreover, streptornase instead DNase was added to the trypsine containing medium and this resulted in a better dispersion of tubule components. The culture conditions remained unmodified. A high degree homogeneity of the cultured cell population and an evident reactivity of these cells to dBcAMP and FSH was achieved. Furthermore, an observation of giant cells, visible in monolayer among the typical Sertoli cells, is discussed in this paper. Contrary to small number of myoid cells, being survived in the culture, these giant cells did not show positive reaction to alkaline phosphatase.

Histoenzymological study of acid phosphatase (GP-AI), 5-nucleotidase (AMP-A), adenosine triphosphatase (ATP-A) and beta-galactosidase (GLAC-A) of the metencephalon of turtle shows a pattern of distribution of enzymes similar to amphibians and mammalian metencephalon which provides indication of homology of the nuclei and tracts such as nucleus raphe, nuclei cerebelli fasciculus longitudinalis medialis, commissura ansulata and internal arcuate fibers. The nerve fibers, tracts and commissures demonstrate strong activity of GLAC-A as demonstrated in frog and bat by the author in previous studies.

The seromucoid level and its neutral sugar composition were studied and the results compared with Golgi-enriched membrane UDP-Gal leads to GlcNAc transferase activity and the morphology of the liver Golgi apparatus in situ. Four groups of rats were studied: 1. control, 2. streptozotocin diabetic, 3. insulin treated, and 4. streptozotocin diabetic and insulin treated. In many respects a correlation was observed between the seromucoid level and composition and the biochemical and morphological properties and composition and the biochemical morphological properties of the Golgi apparatus.