首页 > 最新文献

American journal of physiology. Cell physiology最新文献

英文 中文
Melatonin mitigates matrix stiffness-induced disc degenetation by inhibiting reactive oxygen species and melatonin receptor mediated-PI3K/AKT/NF-κB pathway 褪黑激素通过抑制活性氧和褪黑激素受体介导的PI3K/AKT/NF-κB通路,减轻基质僵化诱发的椎间盘退化
IF 5.5 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-09-09 DOI: 10.1152/ajpcell.00630.2023
Zhihui Liang, Jia Song, Wen-ji Shangguan, Qiu-qi Zhang, Jiang Shao, Yue-hui Zhang
American Journal of Physiology-Cell Physiology, Ahead of Print.
美国生理学-细胞生理学杂志》,提前出版。
{"title":"Melatonin mitigates matrix stiffness-induced disc degenetation by inhibiting reactive oxygen species and melatonin receptor mediated-PI3K/AKT/NF-κB pathway","authors":"Zhihui Liang, Jia Song, Wen-ji Shangguan, Qiu-qi Zhang, Jiang Shao, Yue-hui Zhang","doi":"10.1152/ajpcell.00630.2023","DOIUrl":"https://doi.org/10.1152/ajpcell.00630.2023","url":null,"abstract":"American Journal of Physiology-Cell Physiology, Ahead of Print. <br/>","PeriodicalId":7585,"journal":{"name":"American journal of physiology. Cell physiology","volume":null,"pages":null},"PeriodicalIF":5.5,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142204163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum for Hagen et al., volume 327, 2024, p. C372–C378 Hagen 等人的更正,第 327 卷,2024 年,第 C372-C378 页
IF 5.5 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-09-09 DOI: 10.1152/ajpcell.00326.2024_cor
American Journal of Physiology-Cell Physiology, Volume 327, Issue 3, Page C867-C867, September 2024.
美国生理学杂志-细胞生理学》,第 327 卷第 3 期,第 C867-C867 页,2024 年 9 月。
{"title":"Corrigendum for Hagen et al., volume 327, 2024, p. C372–C378","authors":"","doi":"10.1152/ajpcell.00326.2024_cor","DOIUrl":"https://doi.org/10.1152/ajpcell.00326.2024_cor","url":null,"abstract":"American Journal of Physiology-Cell Physiology, Volume 327, Issue 3, Page C867-C867, September 2024. <br/>","PeriodicalId":7585,"journal":{"name":"American journal of physiology. Cell physiology","volume":null,"pages":null},"PeriodicalIF":5.5,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142204250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Involvement of the gut microbiota in cancer cachexia. 肠道微生物群对癌症恶病质的影响
IF 5 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-07-09 DOI: 10.1152/ajpcell.00327.2024
Brandon N VanderVeen, Thomas D Cardaci, Brooke M Bullard, Michael Madden, Jie Li, Kandy T Velazquez, Jason L Kubinak, Daping Fan, E Angela Murphy

Cancer cachexia, or the unintentional loss of body weight in patients with cancer, is a multiorgan and multifactorial syndrome with a complex and largely unknown etiology; however, metabolic dysfunction and inflammation remain hallmarks of cancer-associated wasting. Although cachexia manifests with muscle and adipose tissue loss, perturbations to the gastrointestinal tract may serve as the frontline for both impaired nutrient absorption and immune-activating gut dysbiosis. Investigations into the gut microbiota have exploded within the past two decades, demonstrating multiple gut-tissue axes; however, the link between adipose and skeletal muscle wasting and the gut microbiota with cancer is only beginning to be understood. Furthermore, the most used anticancer drugs (e.g. chemotherapy and immune checkpoint inhibitors) negatively impact gut homeostasis, potentially exacerbating wasting and contributing to poor patient outcomes and survival. In this review, we 1) highlight our current understanding of the microbial changes that occur with cachexia, 2) discuss how microbial changes may contribute to adipose and skeletal muscle wasting, and 3) outline study design considerations needed when examining the role of the microbiota in cancer-induced cachexia.

癌症恶病质,即癌症患者无意中出现的体重减轻,是一种多器官、多因素综合征,病因复杂且大多不明;然而,代谢功能障碍和炎症仍然是癌症相关消瘦的标志。恶病质表现为肌肉和脂肪组织的损失,而胃肠道的紊乱可能是营养吸收受损和免疫激活性肠道菌群失调的前线。在过去的二十年中,对肠道微生物群的研究呈爆炸式增长,显示出多种肠道组织轴;然而,人们对脂肪和骨骼肌萎缩与肠道微生物群与癌症之间的联系才刚刚开始有所了解。此外,最常用的抗癌药物(如化疗、免疫检查点抑制剂)会对肠道稳态产生负面影响,可能会加剧消瘦并导致患者预后和生存率低下。在本综述中,我们将:1)重点介绍我们目前对恶病质发生的微生物变化的理解;2)讨论微生物变化如何可能导致脂肪和骨骼肌消瘦;3)概述在研究微生物群在癌症诱导的恶病质中的作用时需要考虑的研究设计因素。
{"title":"Involvement of the gut microbiota in cancer cachexia.","authors":"Brandon N VanderVeen, Thomas D Cardaci, Brooke M Bullard, Michael Madden, Jie Li, Kandy T Velazquez, Jason L Kubinak, Daping Fan, E Angela Murphy","doi":"10.1152/ajpcell.00327.2024","DOIUrl":"10.1152/ajpcell.00327.2024","url":null,"abstract":"<p><p>Cancer cachexia, or the unintentional loss of body weight in patients with cancer, is a multiorgan and multifactorial syndrome with a complex and largely unknown etiology; however, metabolic dysfunction and inflammation remain hallmarks of cancer-associated wasting. Although cachexia manifests with muscle and adipose tissue loss, perturbations to the gastrointestinal tract may serve as the frontline for both impaired nutrient absorption and immune-activating gut dysbiosis. Investigations into the gut microbiota have exploded within the past two decades, demonstrating multiple gut-tissue axes; however, the link between adipose and skeletal muscle wasting and the gut microbiota with cancer is only beginning to be understood. Furthermore, the most used anticancer drugs (e.g. chemotherapy and immune checkpoint inhibitors) negatively impact gut homeostasis, potentially exacerbating wasting and contributing to poor patient outcomes and survival. In this review, we <i>1</i>) highlight our current understanding of the microbial changes that occur with cachexia, <i>2</i>) discuss how microbial changes may contribute to adipose and skeletal muscle wasting, and <i>3</i>) outline study design considerations needed when examining the role of the microbiota in cancer-induced cachexia.</p>","PeriodicalId":7585,"journal":{"name":"American journal of physiology. Cell physiology","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11427007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Glucose influences endometrial receptivity to embryo implantation through O-GlcNAcylation-mediated regulation of the cytoskeleton. 葡萄糖通过 O-GlcNAcylation 介导的细胞骨架调节作用影响子宫内膜对胚胎植入的接受能力。
IF 5 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-07-16 DOI: 10.1152/ajpcell.00559.2023
Peter T Ruane, Isabel Paterson, Beth Reeves, Daman Adlam, Stéphane C Berneau, Lewis Renshall, Jan J Brosens, Susan J Kimber, Daniel R Brison, John D Aplin, Melissa Westwood

Phenotypic changes to endometrial epithelial cells underpin receptivity to embryo implantation at the onset of pregnancy but the effect of hyperglycemia on these processes remains poorly understood. Here, we show that physiological levels of glucose (5 mM) abolished receptivity in the endometrial epithelial cell line, Ishikawa. However, embryo attachment was supported by 17 mM glucose as a result of glucose flux through the hexosamine biosynthetic pathway (HBP) and modulation of cell function via protein O-GlcNAcylation. Pharmacological inhibition of HBP or protein O-GlcNAcylation reduced embryo attachment in cocultures at 17 mM glucose. Mass spectrometry analysis of the O-GlcNAcylated proteome in Ishikawa cells revealed that myosin phosphatase target subunit 1 (MYPT1) is more highly O-GlcNAcylated in 17 mM glucose, correlating with loss of its target protein, phospho-myosin light chain 2, from apical cell junctions of polarized epithelium. Two-dimensional (2-D) and three-dimensional (3-D) morphologic analysis demonstrated that the higher glucose level attenuates epithelial polarity through O-GlcNAcylation. Inhibition of Rho (ras homologous)A-associated kinase (ROCK) or myosin II led to reduced polarity and enhanced receptivity in cells cultured in 5 mM glucose, consistent with data showing that MYPT1 acts downstream of ROCK signaling. These data implicate regulation of endometrial epithelial polarity through RhoA signaling upstream of actomyosin contractility in the acquisition of endometrial receptivity. Glucose levels impinge on this pathway through O-GlcNAcylation of MYPT1, which may impact endometrial receptivity to an implanting embryo in women with diabetes.NEW & NOTEWORTHY Understanding how glucose regulates endometrial function will support preconception guidance and/or the development of targeted interventions for individuals living with diabetes wishing to embark on pregnancy. We found that glucose can influence endometrial epithelial cell receptivity to embryo implantation by regulating posttranslational modification of proteins involved in the maintenance of cell polarity. Impaired or inappropriate endometrial receptivity could contribute to fertility and/or early pregnancy complications caused by poor glucose control.

子宫内膜上皮细胞的表型变化是妊娠开始时胚胎着床的接受性的基础,但高血糖对这些过程的影响仍鲜为人知。在这里,我们发现生理水平的葡萄糖(5mM)会降低子宫内膜上皮细胞系石川(Ishikawa)的接受能力。然而,由于葡萄糖通过己糖胺生物合成途径(HBP)和通过蛋白质 O-GlcNAcylation 调节细胞功能,17mM 葡萄糖支持胚胎附着。药物抑制 HBP 或蛋白 O-GlcNAcylation 可减少胚胎在 17mM 葡萄糖条件下的共培养中的附着。对石川细胞中的 O-GlcNAcylated 蛋白质组进行的质谱分析表明,肌球蛋白磷酸酶靶亚基 1 (MYPT1) 在 17mM 葡萄糖中的 O-GlcNAcyl 化程度更高,这与极化上皮细胞顶端细胞连接处其靶蛋白磷酸肌球蛋白轻链 2 的丢失有关。二维和三维形态学分析表明,较高的葡萄糖水平会通过 O-GlcNAcylation 减弱上皮的极性。抑制 RhoA 相关激酶(ROCK)或肌球蛋白 II 会导致在 5mM 葡萄糖中培养的细胞极性减弱、接受性增强,这与 MYPT1 在 ROCK 信号下游发挥作用的数据一致。这些数据表明,在子宫内膜接受性的获得过程中,子宫内膜上皮的极性是通过肌球蛋白收缩性上游的 RhoA 信号调节的。葡萄糖水平通过 MYPT1 的 O-GlcNAcylation 对这一途径产生影响,这可能会影响糖尿病妇女的子宫内膜对植入胚胎的接受能力。
{"title":"Glucose influences endometrial receptivity to embryo implantation through O-GlcNAcylation-mediated regulation of the cytoskeleton.","authors":"Peter T Ruane, Isabel Paterson, Beth Reeves, Daman Adlam, Stéphane C Berneau, Lewis Renshall, Jan J Brosens, Susan J Kimber, Daniel R Brison, John D Aplin, Melissa Westwood","doi":"10.1152/ajpcell.00559.2023","DOIUrl":"10.1152/ajpcell.00559.2023","url":null,"abstract":"<p><p>Phenotypic changes to endometrial epithelial cells underpin receptivity to embryo implantation at the onset of pregnancy but the effect of hyperglycemia on these processes remains poorly understood. Here, we show that physiological levels of glucose (5 mM) abolished receptivity in the endometrial epithelial cell line, Ishikawa. However, embryo attachment was supported by 17 mM glucose as a result of glucose flux through the hexosamine biosynthetic pathway (HBP) and modulation of cell function via protein O-GlcNAcylation. Pharmacological inhibition of HBP or protein O-GlcNAcylation reduced embryo attachment in cocultures at 17 mM glucose. Mass spectrometry analysis of the O-GlcNAcylated proteome in Ishikawa cells revealed that myosin phosphatase target subunit 1 (MYPT1) is more highly O-GlcNAcylated in 17 mM glucose, correlating with loss of its target protein, phospho-myosin light chain 2, from apical cell junctions of polarized epithelium. Two-dimensional (2-D) and three-dimensional (3-D) morphologic analysis demonstrated that the higher glucose level attenuates epithelial polarity through O-GlcNAcylation. Inhibition of Rho (ras homologous)A-associated kinase (ROCK) or myosin II led to reduced polarity and enhanced receptivity in cells cultured in 5 mM glucose, consistent with data showing that MYPT1 acts downstream of ROCK signaling. These data implicate regulation of endometrial epithelial polarity through RhoA signaling upstream of actomyosin contractility in the acquisition of endometrial receptivity. Glucose levels impinge on this pathway through O-GlcNAcylation of MYPT1, which may impact endometrial receptivity to an implanting embryo in women with diabetes.<b>NEW & NOTEWORTHY</b> Understanding how glucose regulates endometrial function will support preconception guidance and/or the development of targeted interventions for individuals living with diabetes wishing to embark on pregnancy. We found that glucose can influence endometrial epithelial cell receptivity to embryo implantation by regulating posttranslational modification of proteins involved in the maintenance of cell polarity. Impaired or inappropriate endometrial receptivity could contribute to fertility and/or early pregnancy complications caused by poor glucose control.</p>","PeriodicalId":7585,"journal":{"name":"American journal of physiology. Cell physiology","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The risks and rewards of the synergist ablation model in skeletal muscle biology research. 协同消融模型在骨骼肌生物学研究中的风险与回报:编辑聚焦
IF 5 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-07-29 DOI: 10.1152/ajpcell.00504.2024
Anika L Syroid, Thomas J Hawke
{"title":"The risks and rewards of the synergist ablation model in skeletal muscle biology research.","authors":"Anika L Syroid, Thomas J Hawke","doi":"10.1152/ajpcell.00504.2024","DOIUrl":"10.1152/ajpcell.00504.2024","url":null,"abstract":"","PeriodicalId":7585,"journal":{"name":"American journal of physiology. Cell physiology","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Skeletal muscle hypertrophy: cell growth is cell growth. 回应反驳啮齿动物增效剂消融模型在确定骨骼肌肥大的分子和细胞机制方面的实用性。
IF 5 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-07-29 DOI: 10.1152/ajpcell.00418.2024
Benjamin I Burke, Ahmed Ismaeel, Ferdinand von Walden, Kevin A Murach, John J McCarthy

Roberts et al. have provided an insightful counterpoint to our review article on the utility of the synergist ablation model. The purpose of this review is to provide some further dialogue regarding the strengths and weaknesses of the synergist ablation model. Specifically, we highlight that the robustness of the model overshadows surgical limitations. We also compare the transcriptomic responses to synergist ablation in mice and resistance exercise in humans to identify common pathways. We conclude that "cell growth is cell growth" and that the mechanisms available to cells to accumulate biomass and increase in size are similar across cell types and independent of the rate of growth.

罗伯茨等人对我们关于协同消融模式的实用性的评论文章提出了有见地的反驳。这篇综述的目的是就协同消融模式的优缺点提供一些进一步的对话。具体来说,我们强调该模型的稳健性掩盖了手术的局限性。我们还比较了小鼠对增效剂消融和人类阻力运动的转录组反应,以确定共同的途径。我们的结论是:"细胞生长就是细胞生长",细胞积累生物量和增大体积的机制在不同类型的细胞中是相似的,与生长速度无关。
{"title":"Skeletal muscle hypertrophy: cell growth is cell growth.","authors":"Benjamin I Burke, Ahmed Ismaeel, Ferdinand von Walden, Kevin A Murach, John J McCarthy","doi":"10.1152/ajpcell.00418.2024","DOIUrl":"10.1152/ajpcell.00418.2024","url":null,"abstract":"<p><p>Roberts et al. have provided an insightful counterpoint to our review article on the utility of the synergist ablation model. The purpose of this review is to provide some further dialogue regarding the strengths and weaknesses of the synergist ablation model. Specifically, we highlight that the robustness of the model overshadows surgical limitations. We also compare the transcriptomic responses to synergist ablation in mice and resistance exercise in humans to identify common pathways. We conclude that \"cell growth is cell growth\" and that the mechanisms available to cells to accumulate biomass and increase in size are similar across cell types and independent of the rate of growth.</p>","PeriodicalId":7585,"journal":{"name":"American journal of physiology. Cell physiology","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Alpha-ketoglutarate is required for chronic hypoxia-induced cardiac remodeling. 慢性缺氧诱导的心脏重塑需要α-酮戊二酸。
IF 5 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-07-29 DOI: 10.1152/ajpcell.00257.2024
Daishi Tang, Yong Gu, Shasha Chen, Tong Niu, Jin'ao Zhu, Panpan Liu, Mingge Ding, Yanjie Guo

Chronic hypoxia (CH) is commonly associated with various cardiovascular diseases, with cardiac hypertrophy being the most frequently observed alteration. Metabolic remodeling is another consequence seen in the hypoxic heart. However, the mechanistic linkage between metabolic remodeling and cardiac hypertrophy in the hypoxic heart remains unclear. In this study, wild-type C57BL/6J mice were subjected to CH for 4 wk. Echocardiography and morphological analysis were used to assess the cardiac effects. We found that 4 wk of CH led to significant cardiac hypertrophy in the mice, whereas cardiac function remained unchanged compared with normoxic mice. In addition, CH induced an elevation in cardiac alpha-ketoglutarate (α-KG) content. Promoting α-KG degradation in the CH hearts prevented CH-induced cardiac hypertrophy but led to noticeable cardiac dysfunction. Mechanistically, α-KG promoted the transcription of hypertrophy-related genes by regulating histone methylation. Silencing lysine-specific demethylase 5 (KDM5), a histone demethylation enzyme, blunted α-KG-induced transcription of hypertrophy-related genes. These data suggest that α-KG is required for CH-induced cardiac remodeling, thus establishing a connection between metabolic changes and cardiac remodeling in hypoxic hearts.NEW & NOTEWORTHY We reported that alpha-ketoglutarate (α-KG) is indispensable for chronic hypoxia (CH)-induced cardiac remodeling, which builds the bridge between metabolic intermediates and cardiac remodeling.

慢性缺氧(CH)通常与各种心血管疾病有关,而心脏肥大是最常观察到的改变。代谢重塑是缺氧性心脏的另一个后果。然而,缺氧心脏中代谢重塑与心脏肥大之间的机理联系仍不清楚。在这项研究中,野生型 C57BL/6J 小鼠接受了为期四周的缺氧治疗。超声心动图和形态学分析被用来评估对心脏的影响。我们发现,与常氧小鼠相比,四周的 CH 会导致小鼠心脏明显肥大,而心脏功能保持不变。此外,CH 还诱导了心脏α-酮戊二酸(α-KG)含量的升高。促进α-KG在CH心脏中的降解可防止CH诱导的心脏肥大,但会导致明显的心脏功能障碍。从机制上讲,α-KG通过调节组蛋白甲基化促进肥大相关基因的转录。抑制组蛋白去甲基化酶赖氨酸特异性去甲基化酶5(KDM5)可减弱α-KG诱导的肥大相关基因的转录。这些数据表明,α-KG 是 CH 诱导的心脏重塑所必需的,从而建立了缺氧心脏代谢变化与心脏重塑之间的联系。
{"title":"Alpha-ketoglutarate is required for chronic hypoxia-induced cardiac remodeling.","authors":"Daishi Tang, Yong Gu, Shasha Chen, Tong Niu, Jin'ao Zhu, Panpan Liu, Mingge Ding, Yanjie Guo","doi":"10.1152/ajpcell.00257.2024","DOIUrl":"10.1152/ajpcell.00257.2024","url":null,"abstract":"<p><p>Chronic hypoxia (CH) is commonly associated with various cardiovascular diseases, with cardiac hypertrophy being the most frequently observed alteration. Metabolic remodeling is another consequence seen in the hypoxic heart. However, the mechanistic linkage between metabolic remodeling and cardiac hypertrophy in the hypoxic heart remains unclear. In this study, wild-type C57BL/6J mice were subjected to CH for 4 wk. Echocardiography and morphological analysis were used to assess the cardiac effects. We found that 4 wk of CH led to significant cardiac hypertrophy in the mice, whereas cardiac function remained unchanged compared with normoxic mice. In addition, CH induced an elevation in cardiac alpha-ketoglutarate (α-KG) content. Promoting α-KG degradation in the CH hearts prevented CH-induced cardiac hypertrophy but led to noticeable cardiac dysfunction. Mechanistically, α-KG promoted the transcription of hypertrophy-related genes by regulating histone methylation. Silencing lysine-specific demethylase 5 (KDM5), a histone demethylation enzyme, blunted α-KG-induced transcription of hypertrophy-related genes. These data suggest that α-KG is required for CH-induced cardiac remodeling, thus establishing a connection between metabolic changes and cardiac remodeling in hypoxic hearts.<b>NEW & NOTEWORTHY</b> We reported that alpha-ketoglutarate (α-KG) is indispensable for chronic hypoxia (CH)-induced cardiac remodeling, which builds the bridge between metabolic intermediates and cardiac remodeling.</p>","PeriodicalId":7585,"journal":{"name":"American journal of physiology. Cell physiology","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cell firing between ON alpha retinal ganglion cells and coupled amacrine cells in the mouse retina. 小鼠视网膜上α视网膜神经节细胞和耦合羊膜细胞之间的细胞发射。
IF 5 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-07-16 DOI: 10.1152/ajpcell.00238.2024
Qin Wang, ChungHim So, Feng Pan

Gap junctions are channels that allow for direct transmission of electrical signals between cells. However, the ability of one cell to be impacted or controlled by other cells through gap junctions remains unclear. In this study, heterocellular coupling between ON α retinal ganglion cells (α-RGCs) and displaced amacrine cells (ACs) in the mouse retina was used as a model. The impact of the extent of coupling of interconnected ACs on the synchronized firing between coupled ON α-RGC-AC pair was investigated using the dopamine 1 receptor (D1R) antagonist-SCH23390 and agonist-SKF38393. It was observed that the synchronized firing between the ON α-RGC-ACs pairs was increased by the D1R antagonist SCH23390, whereas it was eradicated by the agonist SKF38393. Subsequently, the signaling drive was investigated by infecting coupled ON α-RGC-AC pairs with the channelrhodopsin-2(ChR2) mutation L132C engineered to enhance light sensitivities. The results demonstrated that the spikes of ON α-RGCs (without ChR2) could be triggered by ACs (with ChR2) through the gap junction, and vice versa. Furthermore, it was observed that ON α-RGCs stimulated with 3-10 Hz currents by whole cell patch could elicit synchronous spikes in the coupled ACs, and vice versa. This provided direct evidence that the firing of one cell could be influenced by another cell through gap junctions. However, this phenomenon was not observed between OFF α-RGC pairs. The study implied that the synchronized firing between ON α-RGC-AC pairs could potentially be affected by the coupling of interconnected ACs. Additionally, one cell type could selectively control the firing of another cell type, thereby forcefully transmitting information. The key role of gap junctions in synchronizing firing and driving cells between α-RGCs and coupled ACs in the mouse retina was highlighted.NEW & NOTEWORTHY This study investigates the role of gap junctions in transmitting electrical signals between cells and their potential for cell control. Using ON α retinal ganglion cells (α-RGCs) and amacrine cells (ACs) in the mouse retina, the researchers find that the extent of coupling between ACs affects synchronized firing. Bidirectional signaling occurs between ACs and ON α-RGCs through gap junctions.

间隙连接是细胞间直接传输电信号的通道。然而,一个细胞通过间隙连接受其他细胞影响或控制的能力仍不清楚。本研究以小鼠视网膜中ON α视网膜神经节细胞(RGC)和移位的羊膜细胞(AC)之间的异细胞耦合为模型。研究了相互连接的 AC 的耦合程度对耦合 ON α RGC-AC 间同步点火的影响。结果发现,多巴胺1受体拮抗剂SCH23390会增加ON α RGC-ACs对之间的同步点火,而SKF38393则会消除这种同步点火。随后,耦合的ON α RGC-AC对感染了通道色素-2(ChR2)突变L132C。ON α RGC(不含 ChR2)的尖峰可由 AC(含 ChR2)通过间隙连接触发,反之亦然。此外,研究还观察到,通过全细胞贴片以 3-10 Hz 电流刺激 ON α RGC,可引起耦合 AC 的同步尖峰,反之亦然。这项研究表明,ON α RGC-AC 对之间的同步点火有可能受到相互连接的 AC 的耦合影响,另一种细胞类型可以选择性地控制一种细胞类型的点火,信息可以被强力传递。该研究强调了间隙连接在小鼠视网膜中α RGC和耦合AC之间同步点火和驱动细胞的关键作用。
{"title":"Cell firing between ON alpha retinal ganglion cells and coupled amacrine cells in the mouse retina.","authors":"Qin Wang, ChungHim So, Feng Pan","doi":"10.1152/ajpcell.00238.2024","DOIUrl":"10.1152/ajpcell.00238.2024","url":null,"abstract":"<p><p>Gap junctions are channels that allow for direct transmission of electrical signals between cells. However, the ability of one cell to be impacted or controlled by other cells through gap junctions remains unclear. In this study, heterocellular coupling between ON α retinal ganglion cells (α-RGCs) and displaced amacrine cells (ACs) in the mouse retina was used as a model. The impact of the extent of coupling of interconnected ACs on the synchronized firing between coupled ON α-RGC-AC pair was investigated using the dopamine 1 receptor (D1R) antagonist-SCH23390 and agonist-SKF38393. It was observed that the synchronized firing between the ON α-RGC-ACs pairs was increased by the D1R antagonist SCH23390, whereas it was eradicated by the agonist SKF38393. Subsequently, the signaling drive was investigated by infecting coupled ON α-RGC-AC pairs with the channelrhodopsin-2(ChR2) mutation L132C engineered to enhance light sensitivities. The results demonstrated that the spikes of ON α-RGCs (without ChR2) could be triggered by ACs (with ChR2) through the gap junction, and vice versa. Furthermore, it was observed that ON α-RGCs stimulated with 3-10 Hz currents by whole cell patch could elicit synchronous spikes in the coupled ACs, and vice versa. This provided direct evidence that the firing of one cell could be influenced by another cell through gap junctions. However, this phenomenon was not observed between OFF α-RGC pairs. The study implied that the synchronized firing between ON α-RGC-AC pairs could potentially be affected by the coupling of interconnected ACs. Additionally, one cell type could selectively control the firing of another cell type, thereby forcefully transmitting information. The key role of gap junctions in synchronizing firing and driving cells between α-RGCs and coupled ACs in the mouse retina was highlighted.<b>NEW & NOTEWORTHY</b> This study investigates the role of gap junctions in transmitting electrical signals between cells and their potential for cell control. Using ON α retinal ganglion cells (α-RGCs) and amacrine cells (ACs) in the mouse retina, the researchers find that the extent of coupling between ACs affects synchronized firing. Bidirectional signaling occurs between ACs and ON α-RGCs through gap junctions.</p>","PeriodicalId":7585,"journal":{"name":"American journal of physiology. Cell physiology","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The utility of the rodent synergist ablation model in identifying molecular and cellular mechanisms of skeletal muscle hypertrophy. 啮齿动物增效剂消融模型在确定骨骼肌肥大的分子和细胞机制中的作用
IF 5 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-07-29 DOI: 10.1152/ajpcell.00362.2024
Benjamin I Burke, Ahmed Ismaeel, John J McCarthy

Skeletal muscle exhibits remarkable plasticity to adapt to stimuli such as mechanical loading. The mechanisms that regulate skeletal muscle hypertrophy due to mechanical overload have been thoroughly studied. Remarkably, our understanding of many of the molecular and cellular mechanisms that regulate hypertrophic growth were first identified using the rodent synergist ablation (SA) model and subsequently corroborated in human resistance exercise training studies. To demonstrate the utility of the SA model, we briefly summarize the hypertrophic mechanisms identified using the model and the following translation of these mechanism to human skeletal muscle hypertrophy induced by resistance exercise training.

骨骼肌在适应机械负荷等刺激方面具有显著的可塑性。我们对调节骨骼肌因机械超负荷而肥大的机制进行了深入研究。值得注意的是,我们对调控肥大生长的许多分子和细胞机制的了解首先是通过啮齿类动物增效剂消融(SA)模型确定的,随后在人类阻力运动训练研究中得到了证实。为了证明 SA 模型的实用性,我们简要总结了利用该模型确定的肥大机制,以及随后将这些机制转化为阻力运动训练诱导的人类骨骼肌肥大。
{"title":"The utility of the rodent synergist ablation model in identifying molecular and cellular mechanisms of skeletal muscle hypertrophy.","authors":"Benjamin I Burke, Ahmed Ismaeel, John J McCarthy","doi":"10.1152/ajpcell.00362.2024","DOIUrl":"10.1152/ajpcell.00362.2024","url":null,"abstract":"<p><p>Skeletal muscle exhibits remarkable plasticity to adapt to stimuli such as mechanical loading. The mechanisms that regulate skeletal muscle hypertrophy due to mechanical overload have been thoroughly studied. Remarkably, our understanding of many of the molecular and cellular mechanisms that regulate hypertrophic growth were first identified using the rodent synergist ablation (SA) model and subsequently corroborated in human resistance exercise training studies. To demonstrate the utility of the SA model, we briefly summarize the hypertrophic mechanisms identified using the model and the following translation of these mechanism to human skeletal muscle hypertrophy induced by resistance exercise training.</p>","PeriodicalId":7585,"journal":{"name":"American journal of physiology. Cell physiology","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11427019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
EPAC1 and 2 inhibit K+ currents via PLC/PKC and NOS/PKG pathways in rat ventricular cardiomyocytes. EPAC1 和 2 通过 PLC/PKC 和 NOS/PKG 途径抑制大鼠心室心肌细胞中的 K+ 电流。
IF 5 2区 生物学 Q2 CELL BIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-07-10 DOI: 10.1152/ajpcell.00582.2023
Arthur Boileve, Olivier Romito, Thomas Hof, Aurélia Levallois, Laura Brard, Sarah d'Hers, Alexandre Fouchet, Christophe Simard, Romain Guinamard, Fabien Brette, Laurent Sallé

The exchange protein directly activated by cAMP (EPAC) has been implicated in cardiac proarrhythmic signaling pathways including spontaneous diastolic Ca2+ leak from sarcoplasmic reticulum and increased action potential duration (APD) in isolated ventricular cardiomyocytes. The action potential (AP) lengthening following acute EPAC activation is mainly due to a decrease of repolarizing steady-state K+ current (IKSS) but the mechanisms involved remain unknown. This study aimed to assess the role of EPAC1 and EPAC2 in the decrease of IKSS and to investigate the underlying signaling pathways. AP and K+ currents were recorded with the whole cell configuration of the patch-clamp technique in freshly isolated rat ventricular myocytes. EPAC1 and EPAC2 were pharmacologically activated with 8-(4-chlorophenylthio)-2'-O-methyl-cAMP acetoxymethyl ester (8-CPTAM, 10 µmol/L) and inhibited with R-Ce3F4 and ESI-05, respectively. Inhibition of EPAC1 and EPAC2 significantly decreased the effect of 8-CPTAM on APD and IKSS showing that both EPAC isoforms are involved in these effects. Unexpectedly, calmodulin-dependent protein kinase II (CaMKII) inhibition by AIP or KN-93, and Ca2+ chelation by intracellular BAPTA, did not impact the response to 8-CPTAM. However, inhibition of PLC/PKC and nitric oxide synthase (NOS)/PKG pathways partially prevents the 8-CPTAM-dependent decrease of IKSS. Finally, the cumulative inhibition of PKC and PKG blocked the 8-CPTAM effect, suggesting that these two actors work along parallel pathways to regulate IKSS upon EPAC activation. On the basis of such findings, we propose that EPAC1 and EPAC2 are involved in APD lengthening by inhibiting a K+ current via both PLC/PKC and NOS/PKG pathways. This may have pathological implications since EPAC is upregulated in diseases such as cardiac hypertrophy.NEW & NOTEWORHTY Exchange protein directly activated by cAMP (EPAC) proteins modulate ventricular electrophysiology at the cellular level. Both EPAC1 and EPAC2 isoforms participate in this effect. Mechanistically, PLC/PKC and nitric oxide synthase (NO)/PKG pathways are involved in regulating K+ repolarizing current whereas the well-known downstream effector of EPAC, calmodulin-dependent protein kinase II (CaMKII), does not participate. This may have pathological implications since EPAC is upregulated in diseases such as cardiac hypertrophy. Thus, EPAC inhibition may be a new approach to prevent arrhythmias under pathological conditions.

cAMP 直接激活的交换蛋白(EPAC)与心脏促心律失常信号通路有关,包括肌浆网自发的舒张期 Ca2+ 泄漏和离体心室心肌细胞的动作电位持续时间(APD)增加。急性 EPAC 激活后的 AP 延长主要是由于再极化稳态 K+ 电流(IKSS)的减少,但其中的机制仍不清楚。本研究旨在评估 EPAC1 和 2 在 IKSS 下降中的作用,并研究其潜在的信号通路。在新鲜分离的大鼠心室肌细胞中,采用贴片钳技术的全细胞构型记录 AP 和 K+ 电流。用 8-CPTAM (10 µmol/L)对 EPAC1 和 2 进行药理激活,并分别用 R-Ce3F4 和 ESI-05 进行抑制。抑制 EPAC1 和 EPAC2 可明显降低 8-CPTAM 对 APD 和 IKSS 的影响,这表明这两种 EPAC 异构体都参与了这些影响。意想不到的是,用 AIP 或 KN-93 抑制 CaMKII 和用细胞内 BAPTA 螯合 Ca2+ 并不影响对 8-CPTAM 的反应。然而,抑制 PLC/PKC 和 NOS/PKG 通路可部分阻止 8-CPTAM 依赖性的 IKSS 下降。最后,PKC 和 PKG 的累积抑制阻断了 8-CPTAM 的效应,这表明这两种作用者在 EPAC 激活时沿着平行的途径调节 IKSS。基于这些发现,我们认为 EPAC1 和 2 通过 PLC/PKC 和 NOS/PKG 途径抑制 K+ 电流,从而参与了 APD 的延长。这可能具有病理意义,因为 EPAC 在心脏肥大等疾病中上调。
{"title":"EPAC1 and 2 inhibit K<sup>+</sup> currents via PLC/PKC and NOS/PKG pathways in rat ventricular cardiomyocytes.","authors":"Arthur Boileve, Olivier Romito, Thomas Hof, Aurélia Levallois, Laura Brard, Sarah d'Hers, Alexandre Fouchet, Christophe Simard, Romain Guinamard, Fabien Brette, Laurent Sallé","doi":"10.1152/ajpcell.00582.2023","DOIUrl":"10.1152/ajpcell.00582.2023","url":null,"abstract":"<p><p>The exchange protein directly activated by cAMP (EPAC) has been implicated in cardiac proarrhythmic signaling pathways including spontaneous diastolic Ca<sup>2+</sup> leak from sarcoplasmic reticulum and increased action potential duration (APD) in isolated ventricular cardiomyocytes. The action potential (AP) lengthening following acute EPAC activation is mainly due to a decrease of repolarizing steady-state K<sup>+</sup> current (IK<sub>SS</sub>) but the mechanisms involved remain unknown. This study aimed to assess the role of EPAC1 and EPAC2 in the decrease of IK<sub>SS</sub> and to investigate the underlying signaling pathways. AP and K<sup>+</sup> currents were recorded with the whole cell configuration of the patch-clamp technique in freshly isolated rat ventricular myocytes. EPAC1 and EPAC2 were pharmacologically activated with 8-(4-chlorophenylthio)-2'-<i>O</i>-methyl-cAMP acetoxymethyl ester (8-CPTAM, 10 µmol/L) and inhibited with R-Ce3F4 and ESI-05, respectively. Inhibition of EPAC1 and EPAC2 significantly decreased the effect of 8-CPTAM on APD and IK<sub>SS</sub> showing that both EPAC isoforms are involved in these effects. Unexpectedly, calmodulin-dependent protein kinase II (CaMKII) inhibition by AIP or KN-93, and Ca<sup>2+</sup> chelation by intracellular BAPTA, did not impact the response to 8-CPTAM. However, inhibition of PLC/PKC and nitric oxide synthase (NOS)/PKG pathways partially prevents the 8-CPTAM-dependent decrease of IK<sub>SS</sub>. Finally, the cumulative inhibition of PKC and PKG blocked the 8-CPTAM effect, suggesting that these two actors work along parallel pathways to regulate IK<sub>SS</sub> upon EPAC activation. On the basis of such findings, we propose that EPAC1 and EPAC2 are involved in APD lengthening by inhibiting a K<sup>+</sup> current via both PLC/PKC and NOS/PKG pathways. This may have pathological implications since EPAC is upregulated in diseases such as cardiac hypertrophy.<b>NEW & NOTEWORHTY</b> Exchange protein directly activated by cAMP (EPAC) proteins modulate ventricular electrophysiology at the cellular level. Both EPAC1 and EPAC2 isoforms participate in this effect. Mechanistically, PLC/PKC and nitric oxide synthase (NO)/PKG pathways are involved in regulating K<sup>+</sup> repolarizing current whereas the well-known downstream effector of EPAC, calmodulin-dependent protein kinase II (CaMKII), does not participate. This may have pathological implications since EPAC is upregulated in diseases such as cardiac hypertrophy. Thus, EPAC inhibition may be a new approach to prevent arrhythmias under pathological conditions.</p>","PeriodicalId":7585,"journal":{"name":"American journal of physiology. Cell physiology","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141578736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
American journal of physiology. Cell physiology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1