Toluene is a common groundwater contaminant originating from leaks of petroleum products and industrial effluents. Accurately assessing the in-situ biodegradation rate of such contaminants is crucial for evaluating the effectiveness of bioremediation strategies. However, traditional drilling and sampling methods are costly and incapable of in-situ biodegradation rate assessment. In recent years, Spectral induced polarization (SIP) has demonstrated to be an effective tool for real-time monitoring of microbial activity. However, to date, limited researches have investigated its mechanism to real-time monitoring of toluene biodegradation. To address this gap, nine soil column experiments were conducted to monitor the biodegradation of dissolved-phase toluene using the SIP method. Biodegradation was qualitatively confirmed through changes in dissolved oxygen (DO), nitrate (NO3-), and carbon isotope ratios (δ13C). The results indicate that the observed increase in quadrature conductivity primarily reflects bacterial growth during biodegradation rather than variations in dissolved toluene concentration. The maximum specific growth rate () of the inoculated bacteria was estimated to be 0.035 d-1 and the corresponding toluene biodegradation rate constant was 0.018 d-1. These findings demonstrate that SIP offers strong potentials as a quantitative, non-invasive, technique for tracking microbial degradation processes in porous media, providing theoretical and methodological support for future field-scale bioremediation applications.
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