Physiologie (Bucarest)最新文献

The incidence of spontaneous electrical activity during ischemia was studied in normal and spasmophilic subjects with a Bronk electrode inserted in the first dorsal interosseous muscle. Subjects were of both sexes, aged 16 to 65 years. The diagnosis of spasmophilia was established electromyographically by a variant of the Alajouanine test. Ischemia induced in many cases an electrical activity consisting of discharge potentials that have the amplitude, duration and morphology of motor unit potentials. The number of discharging motor units varied, sometimes leading to an electrical Trousseau associated to a carpal spasm. The discharge of individual motor units was irregular and practically uninfluenced by passive manipulations on the muscle or voluntary contraction. The activity started usually 30 sec to 1 min after the onset of ischemia, reached a maximal intensity (as to the number of motor units and frequency of discharge) in about 1 min and declined gradually in the following minutes. This activity was encountered in 55.06% of 770 cases of spasmophilia and only in 11.66% of 283 normal subjects, with a tendency to more intense discharge in spasmophilia. Its incidence in the spasmophilic group was closely related to the intensity of spontaneous repetitive discharges appearing electromyographically after the activation test, reaching 78.63% in most severe cases. The behaviour was interpreted as indicating a higher susceptibility in spasmophilia of axons to depolarization induced by hypoxia. This higher susceptibility appears as an important component of the excitability alterations leading to the complex neuromuscular disorders occurring in spasmophilia.

The effects of acute ethanol intoxication in different rat organs were studied with 32P. Since 32P incorporation in all compounds of cell membrane formation faithfully illustrate their structural and functional integrity, levels of 32P labelled phospholipids, from different organs were investigated in acute experiments. Researches were carried out on male rats, by administering doses of 3.5 and 5.8 g/kg body wt., and killing them at 2, 4, 6, 24, 48, and 72 hours. The response of the liver, brain, kidneys and spleen depends on the administered dose and varies with time.

An overview of recent data concerning the evaluation of overall risk for CHD shows the drawbacks of existing formulae. On this basis a more complete screening score and a new mathematic formula for computing the probability of risk for CHD have been assessed. Besides the primary and classical risk factors the latter also includes psychophysiological variables concerning stress and coronary prone behaviour. Five modalities to estimate the overall risk have been used in cases with various risks and the new formula has been applied to a group of 93 textile workers. The results certify the good differentiation power of the proposed formula.

The radioisotopic measurement of gastric emptying offers information about an aspect of gastric motility. A procedure performed in our laboratory is presented. It is based on the ingestion of 500 ml saline including 1 mCi (3.7 MBq)113m In-DTPA or 99Tc-DTPA. The subject is placed in a half-supine position. Radioactive counts of the tracer are collected with a gammacamera and processed with a microcomputer, using a special program. This offers the histograms of the whole stomach and of the regions of interest (proximal and distal stomach), and also the half-time of these curves. Normal values range between 8-25 min for each curve half-time. The method has applications in physiology, pharmacology and clinical diagnosis.

In 9 out of 11 subjects with upper digestive dyspepsia: pyrosis, nausea, emesis, bloating, with negative roentgenological findings, a radioisotopic test detected a delayed gastric emptying. The investigation was based on a 500 ml saline meal including lmCi (3.7 MBq) 113m In-DTPA. For the sequential detection of the radioactivity in front of the epigastric area a gammacamera was used. This was linked to a microcomputer processing the histograms of the curves and estimating the half-time of the gastric emptying (normal value 8-25 min). The results suggest the need for prokinetic drugs in the management of symptoms due to functional pathology.

Sinus node recovery time, used as an objective parameter of the sinus node function estimation, may be left out in the situation in which an atrial parasystolic focus is present. The overdrive suppression induced through rapid atrial pacing is without effect upon the protected parasystolic focus, and its firing will disturb the post-drive pause. This situation is noticed in two instances and demonstrated in one case.

Starting from the cyclicity of the anginal attack in variant angina, the authors point out the role of alkalosis, besides the richness in alfa-adrenoceptors of the great coronary trunks. At the level of the muscular cell there is a competition between H+-ions and Ca++-ions. The diminution of H+-ions as a result of alkalosis brings about the penetration of Ca++ into the cell and the appearance of the coronary spasm. So, we worked out an original method for the provocation of the spasm (the cold and hiperpneea test) and an original therapeutical procedure (by acetozolamid). We present, herein the first results scored in 16 patients.

The influence of different doses of ethanol on the phospholipid metabolism in rat was investigated with 32P. Male rats received by oral intubation a single intoxicating dose of ethanol (3.5 or 5.8 g/kg body wt) as a 45% solution. The corresponding control rats received an equal volume of water. The rats were killed at 2, 4, 6, 24, 48 and 72 hours. The liver, brain, kidney and spleen responses depend on the administered dose and vary with time.