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Australian journal of biotechnology最新文献

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The US biotechnology industry: an analysis of trends. 美国生物技术产业:趋势分析。
Pub Date : 1988-09-01
M D Dibner
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引用次数: 0
Western Biotechnology Limited. 西方生物科技有限公司
Pub Date : 1988-09-01
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引用次数: 0
Patent battles continue in biotechnology in the United States. 在美国,生物技术领域的专利之争仍在继续。
Pub Date : 1988-09-01
S Irvine
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引用次数: 0
Glutathione S-transferase: a new bacterial expression system. 谷胱甘肽s -转移酶:一种新的细菌表达系统。
Pub Date : 1988-09-01
N Samaras
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引用次数: 0
A competitive inhibition ELISA as a probe for studying the refolding of human serum albumin. 竞争抑制酶联免疫吸附试验(ELISA)用于研究人血清白蛋白的再折叠。
Pub Date : 1988-09-01
J A Clausen, S Reid, D H Randerson

The refolding of iodoacetic acid-blocked human serum albumin (HSA) was studied using a modified competitive inhibition ELISA. A maximum of 89% native activity was detected 24 hours after initiating refolding using an albumin concentration of 600 micrograms/mL. The presence of both monomer and polymer HSA was studied using native polyacrylamide gel electrophoresis of thiol-blocked HSA samples. Monomer HSA was not detected until 2.5 hours after initiating refolding. Fractionated polymer and monomer HSA from a sample trapped at 72 hours after initiating refolding was determined to have 40% and 87% native activity respectively. Both polymer and monomer HSA fractions contribute to the overall immunological activity detected by the ELISA, at various times. The ELISA assay was able to detect the changing HSA conformation associated with refolding of totally reduced HSA.

采用改良的竞争抑制酶联免疫吸附法研究了碘乙酸阻断的人血清白蛋白(HSA)的再折叠。当白蛋白浓度为600微克/毫升时,24小时后检测到89%的天然活性。采用天然聚丙烯酰胺凝胶电泳技术对巯基阻断的人血蛋白样品进行了单体和聚合物人血蛋白的研究。在开始再折叠2.5小时后才检测到单体HSA。在开始再折叠72小时后,从样品中分离出的聚合物和单体HSA分别具有40%和87%的天然活性。在不同的时间,聚合体和单体HSA组分都有助于ELISA检测到的整体免疫活性。ELISA检测能够检测到与完全还原的HSA重折叠相关的HSA构象的变化。
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引用次数: 0
Biotechnology regulation update. 生物技术法规更新。
Pub Date : 1988-09-01
S Meek
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引用次数: 0
On-line information for biotechnologists. 生物技术专家的在线信息。
Pub Date : 1988-09-01
J Affleck
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引用次数: 0
Genetic Manipulation Advisory Committee (GMAC) 基因操作咨询委员会(GMAC)
Pub Date : 1988-09-01
N Millis
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引用次数: 0
Biotechnology information sources. 生物技术信息源。
Pub Date : 1988-05-26 DOI: 10.1300/J122V08N02_10
Julie M. Neway, Arct, Mls
Biotechnology has experienced rapid growth in the past three years. A host of new books, journals, databases, dictionaries and directories have been published to satisfy the information needs of scientific, regulatory, legal and marketing personnel. The biotechnology literature is discussed in this paper under its general, business, scientific, regulatory and patent aspects. Major sources are highlighted in the text, with an extensive source list in the Appendix.
生物技术在过去三年中经历了快速发展。出版了大量的新书、期刊、数据库、词典和目录,以满足科学、监管、法律和营销人员的信息需求。本文从一般、商业、科学、法规和专利等方面对生物技术文献进行了讨论。主要来源在文本中突出显示,附录中有一个广泛的来源列表。
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引用次数: 1
期刊
Australian journal of biotechnology
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