Begüm Güler, Pelin Sağlam Metiner, Sultan GÜLÇE İZ, A. Gürel
{"title":"Overview of 3D Technology Applications in Plants: Phenomic, Mapping with Robotic Systems, Architectural Designs, Plant and Animal Tissue Culture Approaches","authors":"Begüm Güler, Pelin Sağlam Metiner, Sultan GÜLÇE İZ, A. Gürel","doi":"10.18036/aubtdc.378468","DOIUrl":"https://doi.org/10.18036/aubtdc.378468","url":null,"abstract":"","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67555785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A NEW DISTRIBUTION AREA FOR LOCAL ENDEMIC ERODIUM SOMANUM H. PEŞMEN (GERANIACEAE) FROM ARROUND KADRİYE (BURSA)","authors":"Ö. Yilmaz, Aylin Yilmaz, G. Kaynak","doi":"10.18036/aubtdc.417399","DOIUrl":"https://doi.org/10.18036/aubtdc.417399","url":null,"abstract":"","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88610282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Okratoksin A (OTA), bazi Aspergillus ve Penicillium turleri tarafindan sentezlenen ozellikle islenmis tahillar, kahve cekirdekleri veya granul kahveler, sarap ve cesitli baharatlarda bulunabilen bir mikotoksindir. OTA insan ve hayvanlarda kanserojen, mutajenik ve teratojenik etkiler gostermektedir. Gerceklestirilen bu calismada yerel marketlerden temin edilen 30 adet kahve ornegi icerisindeki OTA miktari Yuksek Basincli Sivi Kromatografisi (YPSK) cihazi ile tayin edilmistir. Calismalar floresans dedektor kullanilarak Ex 330 Em 450 nm dalga boylarinda gerceklestirilmis, kolon sicakligi 25°C, sistem basinci 83 bar olarak olculmustur. Akis hizi 0.9 ml/dk olarak secilmistir. Kahveleri cozmek icin etanol kullanilmistir. Analiz boyunca C18 (200 mm 4.6 mm I.D., 5 µm partikul boyutuna sahip kolon kullanilmistir. OTA standardi ve numunelerin yaklasik 11.287 dk da sinyal verdigi gorulmektedir. Mobil faz olarak (su:asetonitril:asetik asit) (49.5:49.5:1) (h:h:h) kullanilmistir. Calisilan 30 adet ornegin tumunde cesitli miktarlarda OTA varligi tespit edilmis olup bunlardan sadece 12 tanesinin Turk Gida Kodeksi tarafindan belirlenen limitler altinda oldugu tespit edilmistir.
{"title":"A GENERAL OVERVIEW OF OCHRATOXIN-A CONTENT OF COFEES SOLD IN LOCAL MARKETS WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHY","authors":"G. İşcan, B. Ergun, M. Soyseven, G. Arli","doi":"10.18036/aubtdc.409099","DOIUrl":"https://doi.org/10.18036/aubtdc.409099","url":null,"abstract":"Okratoksin A (OTA), bazi Aspergillus ve Penicillium turleri tarafindan sentezlenen ozellikle islenmis tahillar, kahve cekirdekleri veya granul kahveler, sarap ve cesitli baharatlarda bulunabilen bir mikotoksindir. OTA insan ve hayvanlarda kanserojen, mutajenik ve teratojenik etkiler gostermektedir. Gerceklestirilen bu calismada yerel marketlerden temin edilen 30 adet kahve ornegi icerisindeki OTA miktari Yuksek Basincli Sivi Kromatografisi (YPSK) cihazi ile tayin edilmistir. Calismalar floresans dedektor kullanilarak Ex 330 Em 450 nm dalga boylarinda gerceklestirilmis, kolon sicakligi 25°C, sistem basinci 83 bar olarak olculmustur. Akis hizi 0.9 ml/dk olarak secilmistir. Kahveleri cozmek icin etanol kullanilmistir. Analiz boyunca C18 (200 mm 4.6 mm I.D., 5 µm partikul boyutuna sahip kolon kullanilmistir. OTA standardi ve numunelerin yaklasik 11.287 dk da sinyal verdigi gorulmektedir. Mobil faz olarak (su:asetonitril:asetik asit) (49.5:49.5:1) (h:h:h) kullanilmistir. Calisilan 30 adet ornegin tumunde cesitli miktarlarda OTA varligi tespit edilmis olup bunlardan sadece 12 tanesinin Turk Gida Kodeksi tarafindan belirlenen limitler altinda oldugu tespit edilmistir.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67555524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SILVER NANOPARTICLE SYNTHESIS WITH HERBAL DRUG EXTRACTS VIA GREEN SYNTHESIS","authors":"P. Nartop","doi":"10.18036/AUBTDC.426828","DOIUrl":"https://doi.org/10.18036/AUBTDC.426828","url":null,"abstract":"","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82225584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EFFECTS OF TIME COURSE SALICYLIC ACID ON THE ANTIOXIDANT DEFENSE SYSTEM IN BARLEY ROOTS UNDER SALT STRESS","authors":"H. Torun, F. Ayaz","doi":"10.18036/AUBTDC.444419","DOIUrl":"https://doi.org/10.18036/AUBTDC.444419","url":null,"abstract":"","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83235304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, peptidase production potential of 14 Bacillus species isolated from ropey breads in a previous study, was determined at 30, 37, 50 and 55 °C. Then, optimum enzyme production medium composition and conditions for two of the strains which were found to have the highest peptidase activity were optimised with one factor at a time approach. For this purpose, carbon source, nitrogen source, carbon/nitrogen ratio, agitation rate and pre-culture medium which ensure the best peptidase activity were determined by considering the cost-effectiveness. The best carbon and nitrogen source was determined as glucose and yeast extract, respectively. Furthermore, higher peptidase activity was obtained with a carbon/nitrogen ratio of 1:5, agitation rate of 250 rpm and with the utilization of enzyme production medium for preparing pre-culture.
{"title":"Determination of peptidase production potential of Bacillus strains isolated from ropey bread and optimisation of some culture conditions for peptidase production","authors":"M. Certel, Fundagül Erem","doi":"10.18036/aubtdc.348383","DOIUrl":"https://doi.org/10.18036/aubtdc.348383","url":null,"abstract":"In this study, peptidase production potential of 14 Bacillus species isolated from ropey breads in a previous study, was determined at 30, 37, 50 and 55 °C. Then, optimum enzyme production medium composition and conditions for two of the strains which were found to have the highest peptidase activity were optimised with one factor at a time approach. For this purpose, carbon source, nitrogen source, carbon/nitrogen ratio, agitation rate and pre-culture medium which ensure the best peptidase activity were determined by considering the cost-effectiveness. The best carbon and nitrogen source was determined as glucose and yeast extract, respectively. Furthermore, higher peptidase activity was obtained with a carbon/nitrogen ratio of 1:5, agitation rate of 250 rpm and with the utilization of enzyme production medium for preparing pre-culture.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67555269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bu calismada Hedysarum cappadocicum turunun anatomisini ortaya koymak amaclanmistir. Hedysarum cappadocicum turu Ic Anadolu ve Dogu Anadolu’da yayilis gosteren endemik bir turdur. Bu calismadaki ornekler Sivas ilinden toplanarak turun tanimi yapildiktan sonra kok, skapus, petiyol ve yaprakciklarin anatomileri arastirilmistir. Ture ait anatomik ozellikler detayli bir sekilde ilk defa bu calismada ortaya konulmustur. Anatomik calismalar sonucunda turun kok enine kesitinde sekonder yapi, skapus enine kesitinin cikintili olusu, tanin iceren izobilateral ve amfistomatik yaprakciklar, uc koseli petiyol ve anizositik stoma tipleri tanimlanmistir.
{"title":"ENDEMİK HEDYSARUM CAPPADOCICUM BOISS. (FABACEAE) TÜRÜNÜN ANATOMİK ÖZELLİKLERİ","authors":"Mehmet Tekin, Yurdanur Akyol","doi":"10.18036/AUBTDC.368393","DOIUrl":"https://doi.org/10.18036/AUBTDC.368393","url":null,"abstract":"Bu calismada Hedysarum cappadocicum turunun anatomisini ortaya koymak amaclanmistir. Hedysarum cappadocicum turu Ic Anadolu ve Dogu Anadolu’da yayilis gosteren endemik bir turdur. Bu calismadaki ornekler Sivas ilinden toplanarak turun tanimi yapildiktan sonra kok, skapus, petiyol ve yaprakciklarin anatomileri arastirilmistir. Ture ait anatomik ozellikler detayli bir sekilde ilk defa bu calismada ortaya konulmustur. Anatomik calismalar sonucunda turun kok enine kesitinde sekonder yapi, skapus enine kesitinin cikintili olusu, tanin iceren izobilateral ve amfistomatik yaprakciklar, uc koseli petiyol ve anizositik stoma tipleri tanimlanmistir.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"7 1","pages":"207-213"},"PeriodicalIF":0.0,"publicationDate":"2018-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47907547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cyclophosphamide is among anticancer and immunosuppressing drugs frequently used in chemotherapy treatment. Cyclophosphamide is a chemotherapeutic agent but has also toxic effect on testes. Testicular cells can be protected from toxic effects by antioxidant systems, including vitamin E. The aim of this study is to reveal what kind of an effect the vit-E exerts upon the experimentally induced CP toxicity on testicular tissue of rats. Results of the present study indicate that the testicular injury induced by CP can be prevented by administration of vitamin E.
{"title":"Histological examination of the role of Vitamin E in cyclophosphamide-induced testicular damage in rats","authors":"D. B. Dönmez, I. Yetim","doi":"10.18036/AUBTDC.364844","DOIUrl":"https://doi.org/10.18036/AUBTDC.364844","url":null,"abstract":"Cyclophosphamide is among anticancer and immunosuppressing drugs frequently used in chemotherapy treatment. Cyclophosphamide is a chemotherapeutic agent but has also toxic effect on testes. Testicular cells can be protected from toxic effects by antioxidant systems, including vitamin E. The aim of this study is to reveal what kind of an effect the vit-E exerts upon the experimentally induced CP toxicity on testicular tissue of rats. Results of the present study indicate that the testicular injury induced by CP can be prevented by administration of vitamin E.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76962391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The protease producing bacteria were screened from Indonesian traditional fermented food, tauco and terasi . During the study, 4 halophilic protease producers were isolated from tauco and terasi . Among these isolates, halophilic bacterial isolate TANN 4 was recorded as the best protease producer. Extracellular protease from isolate TANN 4 was partially purified using ammonium sulfate precipitation. The protease was partially purified with final yield of 72.87 % and 25.41 fold purity. This moderate thermoactive and alkaliphilic protease showed a pH optimum of 8.0 and temperature optimum was 50 °C. The enzyme was also active at salt concentrations ranging from 1 to 15 % (w/v), with optimum activity at 1 % NaCl (w/v). Ethylenediaminetetraacetic acid (EDTA) completely inhibited the enzyme activity suggesting that it was a metalloprotease. Among metal ions, the Ca 2+ , K + and Mg 2+ ions enhanced the activity of enzyme. The K M and Vmax values exhibited by partially purified protease were 0.0649 mM and 216.45 U mg −1 using casein as substrate. The molecular weight was estimated to be 19.8 kDa on SDS PAGE. The enzyme also fairly stable in Triton X-100, SDS, 1 % commercial detergents (OMO and Ariel) and 25 % methanol. This enzyme was capable of hydrolyzing casein, hemoglobin and bovine serum albumin (BSA). Automated ribotyping analysis revealed that 3 isolate (TANN 4, TR 2 and TR 4) resembled Halobacillus trueperi that exhibited 71, 68 and 69 % similarity respectively, and isolate (TR 1) resembled Virgibacillus pantothenticus with 64 % similarity. These characteristics make this halophilic bacterial extracellular metalloprotease seems to be potentially useful for biotechnological and industrial applications.
{"title":"ISOLATION, SCREENING, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE FROM HALOPHILIC BACTERIA ISOLATED FROM INDONESIAN FERMENTED FOOD","authors":"S. Fitriani, K. Guven","doi":"10.18036/aubtdc.322711","DOIUrl":"https://doi.org/10.18036/aubtdc.322711","url":null,"abstract":"The protease producing bacteria were screened from Indonesian traditional fermented food, tauco and terasi . During the study, 4 halophilic protease producers were isolated from tauco and terasi . Among these isolates, halophilic bacterial isolate TANN 4 was recorded as the best protease producer. Extracellular protease from isolate TANN 4 was partially purified using ammonium sulfate precipitation. The protease was partially purified with final yield of 72.87 % and 25.41 fold purity. This moderate thermoactive and alkaliphilic protease showed a pH optimum of 8.0 and temperature optimum was 50 °C. The enzyme was also active at salt concentrations ranging from 1 to 15 % (w/v), with optimum activity at 1 % NaCl (w/v). Ethylenediaminetetraacetic acid (EDTA) completely inhibited the enzyme activity suggesting that it was a metalloprotease. Among metal ions, the Ca 2+ , K + and Mg 2+ ions enhanced the activity of enzyme. The K M and Vmax values exhibited by partially purified protease were 0.0649 mM and 216.45 U mg −1 using casein as substrate. The molecular weight was estimated to be 19.8 kDa on SDS PAGE. The enzyme also fairly stable in Triton X-100, SDS, 1 % commercial detergents (OMO and Ariel) and 25 % methanol. This enzyme was capable of hydrolyzing casein, hemoglobin and bovine serum albumin (BSA). Automated ribotyping analysis revealed that 3 isolate (TANN 4, TR 2 and TR 4) resembled Halobacillus trueperi that exhibited 71, 68 and 69 % similarity respectively, and isolate (TR 1) resembled Virgibacillus pantothenticus with 64 % similarity. These characteristics make this halophilic bacterial extracellular metalloprotease seems to be potentially useful for biotechnological and industrial applications.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90564566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Ilgın, D. Burukoğlu, Merve Baysal, Özlem Hinis, Ö. Atlı
Quetiapine is an atypical antipsychotic drug used for treatments of patients with schizophrenia. Although hepatotoxic effects related to quetiapine treatment were reported in a few studies, potential hepatotoxicity of this drug was not identified clearly. Therefore, it was aimed to evaluate possible hepatotoxic effects of quetiapine by oral administration of this drug at 10 and 20 mg/kg doses to rats for 30 days in our study. For this purpose, plasma aspartate aminotransferase, alanine aminotransferase, total bilirubin and direct bilirubin levels as markers of hepatotoxicity were determined and histopathological examination was performed in liver tissues. According to our results, serum aspartate aminotransferase and alanine aminotransferase levels were significantly increased in quetiapine-administered groups, whereas total and direct bilirubin levels were significantly increased in high dose group. Histopathological examination of liver tissue indicated that necrotic areas were observed in 10 mg/kg quetiapine-administered group whereas necrotic areas were present and sinusoidal dilatation was observed in 20 mg/kg quetiapine-administered group. According to these results, we concluded that quetiapine may induced hepatotoxic effects in rats, dose-dependently.
{"title":"Assessment of Hepatotoxic Effects of Quetiapıne at Repeated Doses in Rats","authors":"S. Ilgın, D. Burukoğlu, Merve Baysal, Özlem Hinis, Ö. Atlı","doi":"10.18036/aubtdc.349864","DOIUrl":"https://doi.org/10.18036/aubtdc.349864","url":null,"abstract":"Quetiapine is an atypical antipsychotic drug used for treatments of patients with schizophrenia. Although hepatotoxic effects related to quetiapine treatment were reported in a few studies, potential hepatotoxicity of this drug was not identified clearly. Therefore, it was aimed to evaluate possible hepatotoxic effects of quetiapine by oral administration of this drug at 10 and 20 mg/kg doses to rats for 30 days in our study. For this purpose, plasma aspartate aminotransferase, alanine aminotransferase, total bilirubin and direct bilirubin levels as markers of hepatotoxicity were determined and histopathological examination was performed in liver tissues. According to our results, serum aspartate aminotransferase and alanine aminotransferase levels were significantly increased in quetiapine-administered groups, whereas total and direct bilirubin levels were significantly increased in high dose group. Histopathological examination of liver tissue indicated that necrotic areas were observed in 10 mg/kg quetiapine-administered group whereas necrotic areas were present and sinusoidal dilatation was observed in 20 mg/kg quetiapine-administered group. According to these results, we concluded that quetiapine may induced hepatotoxic effects in rats, dose-dependently.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76382837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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