Bu calisma ile Karasu Nehri (Tercan, Yedisu, Uzumlu) ve Isikpinar deresi cevresinden olmak uzere dort farkli lokaliteden toplanan gevrek sogut ( Salix fragilis L.) bitkisinden kok, kabuk, yaprak ve yetistikleri toprak orneklerinde (Fe, Cu, Zn, Mn, Pb, Co, Cr, Cd) agir metallerin miktari tespit edildi. Bitki ve toprak ornekleri laboratuvarda on islemlerden gecirildikten sonra agir metal konsantrasyonlarinin ICP-MS’de analizleri yapildi. Farkli lokalitelerden toplanan bitki kisimlarinda ve toprakta elde edilen veriler istatistiksel olarak karsilastirilarak, lokaliteler arasindaki farkliliklar belirlendi. Agir metal konsantrasyonunun bitkide daha cok koklerde biriktigi tespit edildi. Ayrica, agir metal miktarinin diger lokalitelere gore Yedisu lokalitesinde daha fazla oldugu belirlendi. Salix fragilis L. agir metal kirliliginin belirlenmesinde biyomonitor olabilecegi sonucuna varildi.
{"title":"Salix fragilis L. (Gevrek Söğüt)’ in Ağır Metal Kirliliğinde Biomonitor Olarak Kullanılması","authors":"Hasan Yavuzer, Etem Osma","doi":"10.18036/aubtdc.363013","DOIUrl":"https://doi.org/10.18036/aubtdc.363013","url":null,"abstract":"Bu calisma ile Karasu Nehri (Tercan, Yedisu, Uzumlu) ve Isikpinar deresi cevresinden olmak uzere dort farkli lokaliteden toplanan gevrek sogut ( Salix fragilis L.) bitkisinden kok, kabuk, yaprak ve yetistikleri toprak orneklerinde (Fe, Cu, Zn, Mn, Pb, Co, Cr, Cd) agir metallerin miktari tespit edildi. Bitki ve toprak ornekleri laboratuvarda on islemlerden gecirildikten sonra agir metal konsantrasyonlarinin ICP-MS’de analizleri yapildi. Farkli lokalitelerden toplanan bitki kisimlarinda ve toprakta elde edilen veriler istatistiksel olarak karsilastirilarak, lokaliteler arasindaki farkliliklar belirlendi. Agir metal konsantrasyonunun bitkide daha cok koklerde biriktigi tespit edildi. Ayrica, agir metal miktarinin diger lokalitelere gore Yedisu lokalitesinde daha fazla oldugu belirlendi. Salix fragilis L. agir metal kirliliginin belirlenmesinde biyomonitor olabilecegi sonucuna varildi.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87733738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bu calismada bir ileri oksidasyon yontemi olan elektro-oksidasyon prosesinin evsel nitelikli aritma camurlarinin anaerobik curumesi oncesinde bir on aritma islemi olarak kullanilabilirligi degerlendirilmistir. Yurutulen calismada elektro-oksidasyon prosesi, Denizli’de bulunan bir evsel atiksu aritma tesisinden alinan biyolojik camurlara uygulanmistir. Elektro-oksidasyon yonteminde titanyum elektrotlar hem katot hem de anot olarak kullanilmistir. Uygulanan yontemin camur dezentegrasyonu acisindan verimi dezentegrasyon derecesi (DD) parametresi kullanilarak Yanit Yuzey Istatistiksel Deney Metodu ile degerlendirilmistir. Degisken parametreler uygulanan gerilim ve sure olarak secilmistir. En yuksek dezentegrasyon derecesi (%13,9) 30 dakika sureyle 20 volt uygulamasinda elde edilmis olup, elektrolit ilavesinin dezentegrasyon derecesini arttirdigi belirlenmistir. 10 g/L Na 2 SO 4 ilavesi ile dezentegrasyon derecesi %16,6 olarak saptanmistir. Elektro-oksidasyon prosesi uygulamasi sonrasinda camurlarin anaerobik curume islemindeki performanslari Biyokimyasal Metan Potansiyeli (BMP) Testi kullanilarak degerlendirilmistir. BMP Testi sonuclari, elektro-oksidasyon prosesi kullanilarak on aritilan camurun ham camura oranla %11,8 daha fazla metan gazi uretimine neden oldugu tespit edilmistir. Calismadan elde edilen sonuclar, dezentegrasyon amaciyla kullanilan elektro-oksidasyon prosesinin camurlarin anaerobik curume performanslarini arttirdigini gostermistir.
{"title":"ELEKTRO-OKSİDASYON PROSESİ İLE BİYOLOJİK ÇAMUR DEZENTEGRASYONU","authors":"Gülbin Erden","doi":"10.18036/aubtdc.407651","DOIUrl":"https://doi.org/10.18036/aubtdc.407651","url":null,"abstract":"Bu calismada bir ileri oksidasyon yontemi olan elektro-oksidasyon prosesinin evsel nitelikli aritma camurlarinin anaerobik curumesi oncesinde bir on aritma islemi olarak kullanilabilirligi degerlendirilmistir. Yurutulen calismada elektro-oksidasyon prosesi, Denizli’de bulunan bir evsel atiksu aritma tesisinden alinan biyolojik camurlara uygulanmistir. Elektro-oksidasyon yonteminde titanyum elektrotlar hem katot hem de anot olarak kullanilmistir. Uygulanan yontemin camur dezentegrasyonu acisindan verimi dezentegrasyon derecesi (DD) parametresi kullanilarak Yanit Yuzey Istatistiksel Deney Metodu ile degerlendirilmistir. Degisken parametreler uygulanan gerilim ve sure olarak secilmistir. En yuksek dezentegrasyon derecesi (%13,9) 30 dakika sureyle 20 volt uygulamasinda elde edilmis olup, elektrolit ilavesinin dezentegrasyon derecesini arttirdigi belirlenmistir. 10 g/L Na 2 SO 4 ilavesi ile dezentegrasyon derecesi %16,6 olarak saptanmistir. Elektro-oksidasyon prosesi uygulamasi sonrasinda camurlarin anaerobik curume islemindeki performanslari Biyokimyasal Metan Potansiyeli (BMP) Testi kullanilarak degerlendirilmistir. BMP Testi sonuclari, elektro-oksidasyon prosesi kullanilarak on aritilan camurun ham camura oranla %11,8 daha fazla metan gazi uretimine neden oldugu tespit edilmistir. Calismadan elde edilen sonuclar, dezentegrasyon amaciyla kullanilan elektro-oksidasyon prosesinin camurlarin anaerobik curume performanslarini arttirdigini gostermistir.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84268285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cesitli endustriyel ve cevresel faaliyetler sonucunda ortaya cikan ucucu organik bilesikler (UOB’ler), toksik, kanserojen ve biyobirikim ozelliklerine sahip olan organik kimyasallardir. UOB’ler yuksek buhar basinci nedeniyle su ortaminda kolayca yayilabilmekte ve fiziksel ve kimyasal yapilari sayesinde lipit zarlardan gecerek insan DNA’sinda tahribata ve zehirliliklerden oturu de onemli olcude cevre kirliligine neden olmaktadir. Bu sebeple, cevre ve insan yasami acisindan kritik olan su matrisinde, maksimum bulunabilme miktarlari ulusal ve uluslararasi cevre ve saglik orgutleri tarafindan belirlenen bu maddelerin analiz edilmesi ve izlenmesi buyuk onem tasimaktadir. Bu calismada, yapilan literatur calismalari sonucunda, UOB’lerin fiziksel, kimyasal ve toksikolojik ozellikleri, UOB kaynaklari, UOB’lerin ornek hazirlama ve analiz yontemleri ile cevre ve insan sagligi uzerine olan etkileri derlenmistir. Ayrica, calismada deginilen UOB’lerin toksisite degerleri ortaya konarak, cevre ve ozellikle insan sagligi uzerine olasi etkileri aciklanmistir.
{"title":"SULARDA BULUNAN UÇUCU ORGANİK BİLEŞİKLER VE SAĞLIĞA ETKİLERİ","authors":"B. Güzel, Oltan Canli, Elmas Oktem Olgun","doi":"10.18036/aubtdc.401509","DOIUrl":"https://doi.org/10.18036/aubtdc.401509","url":null,"abstract":"Cesitli endustriyel ve cevresel faaliyetler sonucunda ortaya cikan ucucu organik bilesikler (UOB’ler), toksik, kanserojen ve biyobirikim ozelliklerine sahip olan organik kimyasallardir. UOB’ler yuksek buhar basinci nedeniyle su ortaminda kolayca yayilabilmekte ve fiziksel ve kimyasal yapilari sayesinde lipit zarlardan gecerek insan DNA’sinda tahribata ve zehirliliklerden oturu de onemli olcude cevre kirliligine neden olmaktadir. Bu sebeple, cevre ve insan yasami acisindan kritik olan su matrisinde, maksimum bulunabilme miktarlari ulusal ve uluslararasi cevre ve saglik orgutleri tarafindan belirlenen bu maddelerin analiz edilmesi ve izlenmesi buyuk onem tasimaktadir. Bu calismada, yapilan literatur calismalari sonucunda, UOB’lerin fiziksel, kimyasal ve toksikolojik ozellikleri, UOB kaynaklari, UOB’lerin ornek hazirlama ve analiz yontemleri ile cevre ve insan sagligi uzerine olan etkileri derlenmistir. Ayrica, calismada deginilen UOB’lerin toksisite degerleri ortaya konarak, cevre ve ozellikle insan sagligi uzerine olasi etkileri aciklanmistir.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43004165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Yücel, G. Isik, İ. Şengün, Y. Köse, F. Ceylan, B. Demirci, H. Şentürk
In this study, Eskisehir city and its environment were defined as a study area and wild plants consumed as food in this region were selected as research material. According to the study, 28 wild plants consumed as food around Eskisehir were determined. These plants were identified as Amaranthus retroflexus (Kizilbacak), Beta corolliflora (Kir Pancari), Capsella bursa-pastoris (Coban Cantasi), Carduus nutans (Devedikeni), Centaurea urvillei (Coban Cokerten), Chenopodium album (Sirken), Cichorium intybus (Karakavuk), Erodium cicutarium (Coban Ignesi), Galega officinalis (Kecisakali), Glaucium corniculatum (Boynuz Gelincik), Lactuca serriola (Aci Marul), Malva neglecta (Ebegumeci), Nasturtium officinale (Su Teresi), Papaver rhoeas (Gelincik), Papaver somniferum (Hashas), Polygonum lagathifolium (Madimak), Portulaca oleracea (Semizotu), Ranunculus ficaria (Arpaciksalebi), Raphanus raphanistrum (Turpotu), Rumex acetosa (Kuzukulagi), Rumex crispus (Labada), Silene alba (Toklubasi), Stellaria media (Bulgurcuk), Taraxacum officinale (Aciguneyik), Tragopogon dubius (Yemlik), Tragopogon pratensis (Teke Sakali), Urtica dioica (Buyuk Isirgan) and Urtica urens (Kucuk Isirgan). The findings of this study have revealed that the wild plants are an important alternative to well known agricultural products and have important potential for the realization of sustainable rural development.
{"title":"CONSUMPTION TYPES OF WILD PLANTS CONSUMED AS FOOD AROUND ESKIŞEHIR (TURKEY)","authors":"E. Yücel, G. Isik, İ. Şengün, Y. Köse, F. Ceylan, B. Demirci, H. Şentürk","doi":"10.18036/AUBTDC.422306","DOIUrl":"https://doi.org/10.18036/AUBTDC.422306","url":null,"abstract":"In this study, Eskisehir city and its environment were defined as a study area and wild plants consumed as food in this region were selected as research material. According to the study, 28 wild plants consumed as food around Eskisehir were determined. These plants were identified as Amaranthus retroflexus (Kizilbacak), Beta corolliflora (Kir Pancari), Capsella bursa-pastoris (Coban Cantasi), Carduus nutans (Devedikeni), Centaurea urvillei (Coban Cokerten), Chenopodium album (Sirken), Cichorium intybus (Karakavuk), Erodium cicutarium (Coban Ignesi), Galega officinalis (Kecisakali), Glaucium corniculatum (Boynuz Gelincik), Lactuca serriola (Aci Marul), Malva neglecta (Ebegumeci), Nasturtium officinale (Su Teresi), Papaver rhoeas (Gelincik), Papaver somniferum (Hashas), Polygonum lagathifolium (Madimak), Portulaca oleracea (Semizotu), Ranunculus ficaria (Arpaciksalebi), Raphanus raphanistrum (Turpotu), Rumex acetosa (Kuzukulagi), Rumex crispus (Labada), Silene alba (Toklubasi), Stellaria media (Bulgurcuk), Taraxacum officinale (Aciguneyik), Tragopogon dubius (Yemlik), Tragopogon pratensis (Teke Sakali), Urtica dioica (Buyuk Isirgan) and Urtica urens (Kucuk Isirgan). The findings of this study have revealed that the wild plants are an important alternative to well known agricultural products and have important potential for the realization of sustainable rural development.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48403429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lipases are widely distributed in nature and catalyze the hydrolysis of mono, di and tri acyl glycerols to liberate free fatty acids and glycerol. Various microorganisms produce lipases either alone or together with esterase. Lipases form an important group of relevant enzymes and they were used in a variety of biotechnological fields such as food, pharmaceutical, detergent, textile and cosmetic industries. Lipases from lactic acid bacteria may contribute in the development of flavors in foods especially meat products. The goal of the present study is to investigate the ability to produce lipolytic enzymes lactic acid bacteria strains which is selected as potential probiotics and isolated on pastirma samples in our laboratory to determinate the possibilities of using as starter cultures in pastirma production. Lipolytic activity of lactic acid bacteria isolated from pastirma has not been investigated up to now. The plate assay is performed to screening lipolytic activity of lactic acid bacteria isolates. In the study, 3 different growth medium tried and each medium contain tributyrin as a substrate. Using these methods, 50 strains of lactic acid bacteria isolated from Turkish pastirma are screened for the presence of lipolytic activity . As a result of the analysis, the lipolytic activity is observed in 26 tested strains. Lactobacillus plantarum have highest lipolytic activity.
{"title":"Lipolytic Activity of Lactic Acid Bacteria Isolated from Turkish Pastırma","authors":"E. Dinçer, M. Kıvanç","doi":"10.18036/AUBTDC.306292","DOIUrl":"https://doi.org/10.18036/AUBTDC.306292","url":null,"abstract":"Lipases are widely distributed in nature and catalyze the hydrolysis of mono, di and tri acyl glycerols to liberate free fatty acids and glycerol. Various microorganisms produce lipases either alone or together with esterase. Lipases form an important group of relevant enzymes and they were used in a variety of biotechnological fields such as food, pharmaceutical, detergent, textile and cosmetic industries. Lipases from lactic acid bacteria may contribute in the development of flavors in foods especially meat products. The goal of the present study is to investigate the ability to produce lipolytic enzymes lactic acid bacteria strains which is selected as potential probiotics and isolated on pastirma samples in our laboratory to determinate the possibilities of using as starter cultures in pastirma production. Lipolytic activity of lactic acid bacteria isolated from pastirma has not been investigated up to now. The plate assay is performed to screening lipolytic activity of lactic acid bacteria isolates. In the study, 3 different growth medium tried and each medium contain tributyrin as a substrate. Using these methods, 50 strains of lactic acid bacteria isolated from Turkish pastirma are screened for the presence of lipolytic activity . As a result of the analysis, the lipolytic activity is observed in 26 tested strains. Lactobacillus plantarum have highest lipolytic activity.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45010207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to investigate the antimicrobial and antioxidant activities of different extracts from Echinops antayensis C.Vural. Antimicrobial activity was estimated against several common human pathogenic bacterial strains using the agar disc diffusion and minimal inhibitory concentration assays. Antioxidant activity was evaluated using the DPPH radical-scavenging assay and total phenolic content methods. On the light of these experiments, E. antalyensis would seem to be an important natural antioxidant. This study is first report on the biological activity of E. antalyensis as regarded new species from Turkey.
{"title":"BIOACTIVITY RESEARCHING ON NEW SPECIES OF ECHINOPS L. (ASTERACEAE): ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF ECHINOPS ANTALYENSIS","authors":"Handan Şapçı, C. Vural","doi":"10.18036/AUBTDC.333248","DOIUrl":"https://doi.org/10.18036/AUBTDC.333248","url":null,"abstract":"The aim of this study was to investigate the antimicrobial and antioxidant activities of different extracts from Echinops antayensis C.Vural. Antimicrobial activity was estimated against several common human pathogenic bacterial strains using the agar disc diffusion and minimal inhibitory concentration assays. Antioxidant activity was evaluated using the DPPH radical-scavenging assay and total phenolic content methods. On the light of these experiments, E. antalyensis would seem to be an important natural antioxidant. This study is first report on the biological activity of E. antalyensis as regarded new species from Turkey.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42697535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bu calismada, biyodizel eldesi icin yetistirilmekte olan iki farkli Carthamus tinctorius L. (Asteraceae) cesidinin, Remzibey ve Dincer, tohumlari calisma materyali olarak kullanilmistir. Her bir ceside ait tohumlar Petri kaplari icinde, iki kat kurutma kâgidi uzerinde, 16 saat aydinlik/8 saat karanlik fotoperiyotta, 25±1˚C’ta, ucer tekrarli olmak uzere, saf sudan ibaret kontrol ve %2, %4, %8, %15, %50 ve %60 oranlarinda hazirlanmis kalsiyum karbonat solusyonlari icinde cimlenmeye birakilmistir. Elde edilen veriler istatistiksel olarak degerlendirildiginde, Dincer cesidinin Remzibey’e gore kalsiyum karbonata karsi daha dayanikli oldugu tespit edilmistir. Ayrica her iki Carthamus tinctorius cesidinin %8’lik kalsiyum karbonat solusyonunda %4’luk kalsiyum karbonata gore daha iyi cimlenme gosterdigi belirlenmistir. Sonuc olarak Carthamus tinctorius’ un her iki cesidinin de notr topraklarda ve hafif alkali topraklarda daha iyi cimlenebilecegi soylenebilir.
{"title":"FARKLI KONSANTRASYONLARDA KALSİYUM KARBONAT (CaCO3) UYGULAMASININ CARTHAMUS TINCTORIUS L.’A (ASTERACEAE) AİT FARKLI VARYETELERİN TOHUM ÇİMLENMESİ ÜZERİNE ETKİLERİ","authors":"Sema Leblebi̇ci̇, Gül Işik","doi":"10.18036/aubtdc.339180","DOIUrl":"https://doi.org/10.18036/aubtdc.339180","url":null,"abstract":"Bu calismada, biyodizel eldesi icin yetistirilmekte olan iki farkli Carthamus tinctorius L. (Asteraceae) cesidinin, Remzibey ve Dincer, tohumlari calisma materyali olarak kullanilmistir. Her bir ceside ait tohumlar Petri kaplari icinde, iki kat kurutma kâgidi uzerinde, 16 saat aydinlik/8 saat karanlik fotoperiyotta, 25±1˚C’ta, ucer tekrarli olmak uzere, saf sudan ibaret kontrol ve %2, %4, %8, %15, %50 ve %60 oranlarinda hazirlanmis kalsiyum karbonat solusyonlari icinde cimlenmeye birakilmistir. Elde edilen veriler istatistiksel olarak degerlendirildiginde, Dincer cesidinin Remzibey’e gore kalsiyum karbonata karsi daha dayanikli oldugu tespit edilmistir. Ayrica her iki Carthamus tinctorius cesidinin %8’lik kalsiyum karbonat solusyonunda %4’luk kalsiyum karbonata gore daha iyi cimlenme gosterdigi belirlenmistir. Sonuc olarak Carthamus tinctorius’ un her iki cesidinin de notr topraklarda ve hafif alkali topraklarda daha iyi cimlenebilecegi soylenebilir.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43148865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bu calismada Sivas’ta bulunan farkli solar tuzlalardan halofilik mikroorganizmalarin izolasyonu yapilmistir ve elde edilen izolatlarin 16S rRNA gen analizine dayali karakterizasyonu gerceklestirilmistir. Izolasyon islemi farkli tuz konsantrasyonlari ile hazirlanmis 5 farkli besiyeri ile gerceklestirilmistir. Morfolojik olarak farklilik gosteren kolonilerin 16S rRNA genleri ARDRA ile analiz edilmistir ve kesim profilleri karsilastirilmistir. 47 adet sus tanimlama amaciyla secilmistir. Bacteria domaini icerisinde yer alan izolatlarin Idiomarina , Salinivibrio , Halomonas , Salinibacter , Gracilibacillus , Thalassobacillus , Aliifodinibius , Chromohalobacter , Planococcus , Marinobacter , Bacillus , Aquisalimonas , Marinococcus , Alkalibacillus , Kangiella ve Microbulbifer cinsleri ile filogenetik olarak iliskili oldugu saptanmistir. Halofilik arke izolatlari ise Haloferax , Natrinema , Haloterrigena , Halopelagius , Halorhabdus ve Haloarcula cinsleri icerisinde dagilim gostermistir.
在这种钙中，已经通过致幻微生物对Sivas中发现的各种太阳盐进行了分离，并且通过16S rRNA基因分析对产生的隔离器进行了诊断。隔离要求与用不同盐浓度制备的五种不同食物一致。在形态学上，不同gosteren菌落的16S rRNA基因已与ARDRA进行了分析，并在某些图谱中进行了尸体解剖分析。对无氨酸敏感47倍。细菌领域：icerisinde yer alan izolarin Idiomarina，Salinivibrio，Halomonas，Salinibacter，Gracilibacillus，Thalassobacillus，Aliifodinibius，Chromohalobacter，Planococcus，Marinobacter，Bacillus，Aquisalimas，Marincoccus，Alkalibacillus和Kangiella ve Microbulbifer cinsleri ile filtogenetik olaak iliskili oldugu saptanmistir。Halopherax、Natrinema、Haloterigena、Halopelagius、Halorhabdus和Haloarcula是冰山中最复杂的物种。
{"title":"SİVAS’IN SOLAR TUZLALARINDAKİ MİKROBİYAL TOPLULUKLARIN KÜLTÜR-BAĞIMLI KARAKTERİZASYONU","authors":"M. Mutlu, S. Çinar","doi":"10.18036/aubtdc.305855","DOIUrl":"https://doi.org/10.18036/aubtdc.305855","url":null,"abstract":"Bu calismada Sivas’ta bulunan farkli solar tuzlalardan halofilik mikroorganizmalarin izolasyonu yapilmistir ve elde edilen izolatlarin 16S rRNA gen analizine dayali karakterizasyonu gerceklestirilmistir. Izolasyon islemi farkli tuz konsantrasyonlari ile hazirlanmis 5 farkli besiyeri ile gerceklestirilmistir. Morfolojik olarak farklilik gosteren kolonilerin 16S rRNA genleri ARDRA ile analiz edilmistir ve kesim profilleri karsilastirilmistir. 47 adet sus tanimlama amaciyla secilmistir. Bacteria domaini icerisinde yer alan izolatlarin Idiomarina , Salinivibrio , Halomonas , Salinibacter , Gracilibacillus , Thalassobacillus , Aliifodinibius , Chromohalobacter , Planococcus , Marinobacter , Bacillus , Aquisalimonas , Marinococcus , Alkalibacillus , Kangiella ve Microbulbifer cinsleri ile filogenetik olarak iliskili oldugu saptanmistir. Halofilik arke izolatlari ise Haloferax , Natrinema , Haloterrigena , Halopelagius , Halorhabdus ve Haloarcula cinsleri icerisinde dagilim gostermistir.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44832219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Due to the increasing demand and over collection of orchids from nature to produce salep, scientists have been led to search for more efficient ways to propagate these specific orchids in vitro . This present study compares germination performances of two commercial (Orchimax and Knudson C) and one specially prepared orchid growth media (SV), on economically and medicinally important orchids used to make salep; ( Anacamptis pyramidalis , Dactylorhiza romana , Neotinea tridentata and Anacamptis morio subsp. morio ) and further aims to obtain a mature orchid plant by following the natural environmental cycle. Significant differences in seed germination and protocorm development were observed. Asymbiotic germination tests showed that the specially prepared growth media performed better than the commercial media by 79.11% germination rate. Also, that A. morio subsp. morio had the best germination rate by 88.91%. Protocorms developed in the sixteenth week after sowing. Soil was collected from the natural habitat of each species and was used as a potting substrate, and this helped orchids to pass their initial acclimatization stage. Regeneration success of orchids at in vitro conditions could be increased by using SV growth medium, following their natural hardening off cycle and using specific substrates from their respective habitats.
{"title":"Comparative Growth Media Performances on in vitro Propagation of Some Salep Orchids","authors":"Yasemin Kemeç Hürkan, Kaan Hürkan, C. Aki","doi":"10.18036/AUBTDC.331328","DOIUrl":"https://doi.org/10.18036/AUBTDC.331328","url":null,"abstract":"Due to the increasing demand and over collection of orchids from nature to produce salep, scientists have been led to search for more efficient ways to propagate these specific orchids in vitro . This present study compares germination performances of two commercial (Orchimax and Knudson C) and one specially prepared orchid growth media (SV), on economically and medicinally important orchids used to make salep; ( Anacamptis pyramidalis , Dactylorhiza romana , Neotinea tridentata and Anacamptis morio subsp. morio ) and further aims to obtain a mature orchid plant by following the natural environmental cycle. Significant differences in seed germination and protocorm development were observed. Asymbiotic germination tests showed that the specially prepared growth media performed better than the commercial media by 79.11% germination rate. Also, that A. morio subsp. morio had the best germination rate by 88.91%. Protocorms developed in the sixteenth week after sowing. Soil was collected from the natural habitat of each species and was used as a potting substrate, and this helped orchids to pass their initial acclimatization stage. Regeneration success of orchids at in vitro conditions could be increased by using SV growth medium, following their natural hardening off cycle and using specific substrates from their respective habitats.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49465648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kok hucreler, ilac/toksisite taramalarinda, hastalik yolaklarinin arastirilmasi ve gelisimsel toksisite gibi mekanistik calismalarda halihazirda kullanilmaktadir. Insan-induklenmis pluripotent kok hucreleri (iPSC) farklilasmis, olgun ve ozellesmis hucrelerden pluripotensi genlerinin manuplasyonu ile elde edilebilmekte ve bu sayede kisinin genetik yapisini yansitabildigi gibi cevresel maruziyetin neden oldugu toksisite durumlari da belirlenebilmektedir. Insan embriyonik kok hucreleri (hESC) ve iPSC’lerin genomik, proteomik, transkripteomik ve metabolomik kaliplari kapsamli olarak cikartilarak; hastalik ve toksisite durumlarinin altinda yatan yolaklarin aydinlatilmasi saglanabilir. Her ne kadar teknolojik zorluklar ve etik engeller yaygin kullanimlarinin onune gecse de ilac kesiflerinde ve toksisite calismalarinda insan kok hucreleri temelli sistemlerin kullanimi, daha guvenli kisisellestirilmis ilaclarin uretilmesine ve cevresel toksikanlar icin daha dogru risk degerlendirmelerinin yapilmasina, maliyet ve deney hayvani kullaniminin azaltilmasina olanak saglayabilmektedir. Bu derlemede kok hucrelerin toksikoloji calismalarindaki kullanimlarindan bahsedilmistir.
{"title":"Kök Hücrelerin Toksikoloji Çalışmalarında Kullanımı","authors":"C. Yalçın, S. Yılmaz","doi":"10.18036/aubtdc.334686","DOIUrl":"https://doi.org/10.18036/aubtdc.334686","url":null,"abstract":"Kok hucreler, ilac/toksisite taramalarinda, hastalik yolaklarinin arastirilmasi ve gelisimsel toksisite gibi mekanistik calismalarda halihazirda kullanilmaktadir. Insan-induklenmis pluripotent kok hucreleri (iPSC) farklilasmis, olgun ve ozellesmis hucrelerden pluripotensi genlerinin manuplasyonu ile elde edilebilmekte ve bu sayede kisinin genetik yapisini yansitabildigi gibi cevresel maruziyetin neden oldugu toksisite durumlari da belirlenebilmektedir. Insan embriyonik kok hucreleri (hESC) ve iPSC’lerin genomik, proteomik, transkripteomik ve metabolomik kaliplari kapsamli olarak cikartilarak; hastalik ve toksisite durumlarinin altinda yatan yolaklarin aydinlatilmasi saglanabilir. Her ne kadar teknolojik zorluklar ve etik engeller yaygin kullanimlarinin onune gecse de ilac kesiflerinde ve toksisite calismalarinda insan kok hucreleri temelli sistemlerin kullanimi, daha guvenli kisisellestirilmis ilaclarin uretilmesine ve cevresel toksikanlar icin daha dogru risk degerlendirmelerinin yapilmasina, maliyet ve deney hayvani kullaniminin azaltilmasina olanak saglayabilmektedir. Bu derlemede kok hucrelerin toksikoloji calismalarindaki kullanimlarindan bahsedilmistir.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49466577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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