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Design of a bacterial system using Escherichia coli to detect the mutagenic effect of some drugs 利用大肠杆菌检测某些药物致突变作用的细菌系统的设计
Pub Date : 2023-02-15 DOI: 10.29350/qjps.2022.27.1.1604
Abbas Mayar Hezam
The current study aimed to use the auxotroph's tryptophan Escherichia coli as a test to detect Ibuprofen's mutagenic potential. the Ames test was used to detect reverse mutations in auxotroph's E. coli treated with Ibuprofen. The results showed a significant increase (P< 0.05) in reverse colonies of auxotroph's E. coli treated with 250 and 500 mg/mL of Ibuprofen compared to the negative control. The polymerase chain reaction and DNA Sequencing were used to detect the reverse mutations in the try gene of the auxotroph's E. coli. The results showed the presence of the try gene in all isolates treated with 250 and 500 mg/mL of Ibuprofen at a percentage of (100%). The try gene was sequenced using BLAST software and compared to the gene sequence of a standard isolate. The genetic variation analysis of the try gene isolates treated with Ibuprofen revealed that point mutations in the DNA of the try gene altered protein translation.
目前的研究旨在利用营养不良的大肠杆菌的色氨酸作为检测布洛芬致突变潜力的测试。艾姆斯试验被用来检测用布洛芬治疗的营养不良大肠杆菌的反向突变。结果表明,与阴性对照相比,250和500 mg/mL布洛芬处理的营养不良大肠杆菌反向菌落数量显著增加(P< 0.05)。采用聚合酶链反应法和DNA测序法检测缺陷型大肠杆菌中try基因的反向突变。结果表明,用250和500 mg/mL布洛芬处理的所有分离株中均存在try基因(100%)。利用BLAST软件对try基因进行测序,并与标准分离物的基因序列进行比较。对经布洛芬处理的try基因分离株的遗传变异分析表明,try基因DNA的点突变改变了蛋白质的翻译。
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引用次数: 0
Molecular characterization of Proteus mirabilis virulence factors isolated from patients with otitis media in Diwaniyah, Iraq 伊拉克迪瓦尼耶中耳炎患者分离的奇异变形杆菌毒力因子的分子特征
Pub Date : 2023-02-15 DOI: 10.29350/qjps.2021.26.5.1605
Abbas Mayar Hezam
The current study's goal was to identify virulence factors (genes) in P. mirabilis. A total of 25/100 (25%) Proteus mirabilis were obtained from patients with otitis media and identified using culture and biochemical characteristics, and Polymerase chain reaction (PCR). The PCR technique was used to detect the 16S rRNA gene and all virulence factors, including the ureC gene, which encodes urease synthesis, the flaA gene, which encodes flagella and fimbriae production, the hpmA gene, which encodes hemolysin production, and the zapA gene, which encodes protease. The results revealed that Proteus mirabilis isolates have 100% of the 16S rRNA gene, 100% of the ureC gene, 95% of the flaA gene, 95% of the hpmA gene, and 100% of the zapA gene.
目前的研究目标是鉴定奇异单胞杆菌的毒力因子(基因)。从中耳炎患者中获得25/100(25%)奇异变形杆菌,并通过培养、生化特征和聚合酶链反应(PCR)进行鉴定。采用PCR技术检测16S rRNA基因及所有毒力因子,包括编码脲酶合成的ureC基因、编码鞭毛和毛生成的flaA基因、编码溶血素生成的hpmA基因和编码蛋白酶的zapA基因。结果显示,奇异变形杆菌分离物具有100%的16S rRNA基因、100%的ureC基因、95%的flaA基因、95%的hpmA基因和100%的zapA基因。
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引用次数: 0
Soft i-Open Sets in Soft Bi-Topological Spaces 软双拓扑空间中的软i-开集
Pub Date : 2023-01-21 DOI: 10.29350/qjps.2022.27.1.1592
Sabih Askandar, Beyda S. Abdullah, L. Khaleel
In our study we introduced soft i-open sets and soft i-star-generalized-w-closed sets in soft bi-topological spaces, , using the notion of soft i-open sets in soft-topological-space, . We besides that give examples to clarify these relationships while presenting some essential characteristics and relationships between various groups of sets. Besides that we studied the property of soft bi-topologically extended and non-soft bi-topologically extended of soft i-open sets in soft bi-topological spaces by proofs and examples.
本文利用软拓扑空间中的软i-开集的概念,在软双拓扑空间中引入了软i-开集和软i-星广义-w-闭集。此外,我们还举例说明了这些关系,同时给出了不同集合群之间的一些基本特征和关系。此外,通过证明和实例研究了软双拓扑空间中软i开集的软双拓扑扩展和非软双拓扑扩展的性质。
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引用次数: 0
S A Review of DES and AES algorithms for image Encryption DES和AES图像加密算法综述
Pub Date : 2023-01-17 DOI: 10.29350/qjps.2022.27.1.1512
Suhad Muhajer
  Today the encryption of images has be a requirement to protect a secret image from unauthorized arrival. Different techniques have been presented and sophisticated in spatial, frequency and hybrid domains to encrypt the image securely. Depending on on the security requirements, encryption methods can be classified as full encryption or partial encryption. This paper proposes a review of several image encryption methods  based on AES and DES. The results of previous research have shown that good results in many security analysis such as time execution, entropy, differential attacks, histogram and others. Comparison between AES and DES based on number of factors are presented in this work.
今天,图像加密已经成为保护秘密图像免受未经授权到达的要求。在空间域、频率域和混合域提出了不同的技术来实现图像的安全加密。根据安全性要求,加密方法可分为完全加密和部分加密。本文综述了基于AES和DES的几种图像加密方法,前人的研究结果表明,它们在时间执行、熵、差分攻击、直方图等安全分析方面都取得了良好的效果。在此工作中,提出了AES和DES基于若干因素的比较。
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引用次数: 0
Comparative physiology study of side effect between Xenical and Lipo-6 Supplements which treated obese rabbets Xenical和lipo6补充剂治疗肥胖家兔副反应的比较生理学研究
Pub Date : 2022-07-18 DOI: 10.29350/qjps.2022.27.1.1542
H. G. Abdulshaheed
Obesity is rapidly becoming a health problem, and it is considered a metabolic disease of epidemic proportions. Obesity is also associated with many comorbidities such as hyperlipidemia, fatty liver, atherosclerosis, cancer. Use of over-the-counter weight-loss supplements has become more common in the world; Xenical and Lipo-6 are two examples of nutritional supplements that do not pass safety research before being sold to the consumer. The ability of Xenical and lipo-6 supplements on DNA fragmentation in human lymphocytes was studied, where these cells represent important defense line of the body. On other hand, thirty white rabbits Iraqis in this experiment, weighing 2.5 to 3.0 kg were employed, which divided randomly to the three group (A: Control group, B: Xenical group, C: Lipo-6 group). In the end of experiment, all animals synthesized for collection blood to complete blood count examination and then all animal scarified for taking tissues samples to histopathological examination. The results showed that, the effects of two different types of medications on DNA fragmentation in human lymphocytes were examined. Xenical, at high concentrations (500 µg/ml), caused a significant fragmentation of lymphocytes' DNA (p ≥ 0.05); DNA fragmentation percentages were 68.5 percent after 24 hours and reached 76.4 percent after 48 hours. the lipo-6 at high concentration displayed apoptotic activity against healthy cells after 24 hours of exposure; DNA fragmentation was 80.13%. After 48 hours, the proportion of DNA fragmentation increased to 89.71 percent. As well as, the present study were conducting to determine toxicity of drug in rabbits in order to scientific information about its safety. The results of this study, reveals a histological sections for (liver, lung, heart, spleen, and intestine) in rabbits groups treated with Xenical and Lipo- 6 two capsule per day. pathological alterations in the treated rabbits' intestines, including macrophage and lymphocyte infiltration indicating chronic inflammation, furthermore, intestine section showed Macro vesicular fatty change. The spleen histological Samples founded with highly-blood foci (bleeding), highly infiltration with lymphocytes, also section exhibit highly proliferative cells with abnormal blood collections in both groups treated with Xenical and Lipo-6,in comparative to normal spleen. the lung tissue inflammation, the production of air vacuoles, damage to the alveolar sac and found a significant RBC cast in the heart muscle.
肥胖正迅速成为一个健康问题,它被认为是一种流行病的代谢性疾病。肥胖还与许多合并症有关,如高脂血症、脂肪肝、动脉粥样硬化、癌症。使用非处方减肥补品在世界上变得越来越普遍;Xenical和lipo6是两个在出售给消费者之前没有通过安全研究的营养补充剂的例子。研究了Xenical和脂-6补充剂对人体淋巴细胞DNA断裂的影响,这些细胞是人体的重要防线。另一方面,选用体重2.5 ~ 3.0 kg的伊拉克大白兔30只,随机分为3组(A:对照组,B:赛尼可组,C:脂-6组)。实验结束后,所有动物合成采血完成血细胞计数检查,然后将所有动物切片取组织标本进行组织病理学检查。结果表明,两种不同类型的药物对人淋巴细胞DNA断裂的影响进行了检测。高浓度(500µg/ml)的赛尼可导致淋巴细胞DNA明显断裂(p≥0.05);24小时后DNA断裂率为68.5%,48小时后达到76.4%。高浓度脂-6暴露24小时后对健康细胞表现出凋亡活性;DNA断裂率为80.13%。48h后,DNA断裂比例增加到89.71%。同时,本研究还进行了药物对家兔的毒性测定,为其安全性提供科学依据。本研究结果显示,每天服用两粒Xenical和Lipo- 6胶囊的家兔组(肝、肺、心、脾和肠)的组织学切片。治疗后家兔肠道病理改变,巨噬细胞和淋巴细胞浸润显示慢性炎症,肠切片显示大泡性脂肪改变。脾脏组织学样品发现高度血灶(出血),淋巴细胞高度浸润,切片显示高度增殖细胞,与正常脾脏相比,Xenical和lipop -6治疗组的血液收集异常。肺组织炎症,产生空泡,肺泡囊受损,心肌中发现明显的红细胞铸型。
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引用次数: 0
Candida spp. associated with COVID-19 and its Susceptibility to some Antifungals 与COVID-19相关的念珠菌及其对某些抗真菌药物的敏感性
Pub Date : 2022-07-08 DOI: 10.29350/qjps.2022.27.1.1517
Mohammed M. Alkhuzaie, N. Jasim
This study aimed to conduct a survey of the Candida species associated with COVID-19 viral infection in 150 patients who were admitted to the intensive care unit (ICU) in Al-Diwaniyah Teaching Hospital in Al-Diwaniyah City, Iraq, for a five-month period from October 2021 to February 2022. The results indicated the dominance of Candida spp. over the rest of the isolated fungal species, with 97 isolates (64.66%). C. albicans was shown to be the most abundant species with a percentage of 55.67 percent (P 0.05), compared to the other Candida species that were isolated (Candida tropicalis 13.4 percent, Candida glabrata 12 percent, Candida krusie and Candida parapsilosis with 9.28 percent). In addition, we brought attention to the excellent action of the antifungals amphotericin B, itraconazole, and voriconazole, all of which have a high susceptibility rate.
本研究旨在对伊拉克Al-Diwaniyah市Al-Diwaniyah教学医院重症监护病房(ICU)收治的150名患者中与COVID-19病毒感染相关的念珠菌物种进行调查,为期5个月,从2021年10月至2022年2月。结果表明,念珠菌属真菌以97株(64.66%)居首位。其中,白色念珠菌数量最多,占55.67% (P < 0.05),其余念珠菌分别为热带念珠菌13.4%、光秃念珠菌12%、克鲁丝念珠菌和假丝念珠菌9.28%。此外,我们注意到抗真菌药物两性霉素B、伊曲康唑和伏立康唑的良好作用,它们都有很高的敏感性。
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引用次数: 0
Environmental and diagnostic study of some parasites in Barbus Xanthopterus fish of Al-Diwaniyah River Al-Diwaniyah河Barbus Xanthopterus鱼中某些寄生虫的环境和诊断研究
Pub Date : 2022-06-29 DOI: 10.29350/qjps.2022.27.1.1533
Abtisam Jasim Abbas
     The current study was concerned with investigating parasitic infections in Barbus Xanthopterus fish in Al-Diwaniyah River, for the period (July 2020-December 2020). (218) fish were collected from three different locations along the Al-Diwaniyah River. The results of the laboratory examination indicated that the fish were infected with the parasite Chilodonella Cyprinid and Eimiria sinenis. The current study recorded changes in the physical and chemical properties of Al-Diwaniyah River water, as the water temperature values, measured turbidity values, pH, electrical conductivity, hardness and TDS  recorded a clear variation during the months of the study. The results of the statistical analysis indicated that there were significant differences between these variables at a level of probability (P≤0.05).
目前的研究涉及在2020年7月至2020年12月期间调查Al-Diwaniyah河Barbus Xanthopterus鱼的寄生虫感染。在Al-Diwaniyah河沿岸的三个不同地点收集了218条鱼。实验室检查结果表明,该鱼感染了塞浦路斯赤络菌和中华艾氏体寄生虫。目前的研究记录了Al-Diwaniyah河水的物理和化学性质的变化,因为水温值、测量的浊度值、pH值、电导率、硬度和TDS在研究的几个月中记录了明显的变化。统计分析结果显示,这些变量之间在概率水平上有显著差异(P≤0.05)。
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引用次数: 0
Antibiotic susceptibility of Proteus Mirabillis that isolates of Diabetic foot ulcers in Al- Diwaniyah Hospital 在Al- Diwaniyah医院分离糖尿病足溃疡的奇异变形杆菌的抗生素敏感性
Pub Date : 2022-06-17 DOI: 10.29350/qjps.2022.27.1.1528
H. Jaber, S. Fazaa, Almiyah
The current study have been included 250 samples diabetic foot ulcer patients attending in Al-Diwaniyah Teaching Hospital and private clinics at a period of study from beginning October 2021 to February 2022, Isolates were identified by morphological form on blood agar and macConkey agar, traditional biochemical tests and then confirmed by Vitek 2 system.
本研究纳入了2021年10月至2022年2月期间在Al-Diwaniyah教学医院和私人诊所就诊的250例糖尿病足溃疡患者样本,通过血琼脂和macConkey琼脂的形态鉴定、传统生化检测和Vitek 2系统确认分离株。
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引用次数: 2
The Refractive Index Of Photonic Crystal Fibers As A Function Of Some Parameters And Temperature 光子晶体光纤的折射率与某些参数和温度的关系
Pub Date : 2022-06-06 DOI: 10.29350/qjps.2022.27.2.1473
Mohamةed Altaie
Photonic crystal fibers in the late period occupied a wide range of studies and research because of the ease of dealing with them in terms of design and installation, as there is a group of parameters used in them that can affect the refractive index of the pulse propagation through it, including the diameter of the air holes, the distance between the air holes and their number, as this study showed in addition to the aforementioned parameters The effect of temperature on the refractive index was also studied. It has been observed that with an increase in the diameter of the air holes, the refractive index increases and, conversely, the increase in the distance between them leads to a decrease in the refractive index. As for the number of air holes, it has no clear effect. As for the temperature, which is proportional to the frequency and intensity, this increase in temperature leads to an increase in the refractive index of the pulse passing through the photonic crystal fiber. Changing the temperature of the photonic crystal fiber is an interesting for dynamics fine refractive index tuning in active refractive index shift compensation system.  this paper  present a numerical analysis on the effect of photonic crystal fiber temperature on refractive index and modal features. the research depend on regular hexagonal crystal lattice fibers with specific geometric parameters using finite element method.
后期的光子晶体光纤由于在设计和安装上易于处理,因此得到了广泛的研究和研究,因为光子晶体光纤中使用了一组影响脉冲通过它的折射率的参数,包括气孔的直径、气孔之间的距离和气孔的数量;研究结果表明,除了上述参数外,还研究了温度对折射率的影响。已经观察到,随着气孔直径的增加,折射率增加,相反,气孔之间距离的增加导致折射率降低。对于气孔的数量,没有明显的影响。至于温度,它与频率和强度成正比,温度的增加导致脉冲通过光子晶体光纤的折射率增加。在主动折射率偏移补偿系统中,改变光子晶体光纤的温度是动态精细折射率调谐的一个研究方向。本文对光子晶体光纤温度对折射率和模态特性的影响进行了数值分析。采用有限元法对具有特定几何参数的正六方晶格纤维进行研究。
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引用次数: 0
Correlation and comparison of some trace elements and their related proteins in Type 2 Diabetes mellitus in Kalars city/ Kurdistan-Iraq. 卡拉尔市/库尔德斯坦-伊拉克地区2型糖尿病患者微量元素及其相关蛋白的相关性及比较
Pub Date : 2022-05-23 DOI: 10.29350/qjps.2022.27.2.1493
Helal Muhammed, b-Zeyan Abdullah
The serum levels of trace elements alterations have been linked with Type 2 Diabetes mellitus (T2 DM) and its complication. The Objectives of this study is to detect the correlation relation between serum Chromium, Magnesium (Mg), Zinc (Zn), Copper (Cu), and Iron (Fe) with Fasting Blood Sugar (FBS) in patients with type two diabetes mellitus, also to study the comparison of Ferroxidase, Transferrin, and Total iron binding capacity in patients with type two diabetes mellitus with control groups. A total of forty subjects were included in this study, they were divided in two groups (control and diabetic patients). Fasting blood samples were taken from all participants and analyzed for levels of Cr, Mg, Zn, Cu, and Fe. The concentration of serum trace elements of each sample was determined by using Inductively Coupled Plasma Optical Emission Spectroscopy. A negative correlation was found between Mg, Zn, and Fe level in Type-2 diabetics with FBS, whereas serum copper level shows significant positive correlation with FBS, and chromium shown no significant correlation with FBS. Ferroxidase, and Total binding capacity showed a significant increase, while a significant decrease was found in Transferrin of T2DM.
血清微量元素水平的改变与2型糖尿病(T2 DM)及其并发症有关。本研究的目的是检测2型糖尿病患者血清铬、镁(Mg)、锌(Zn)、铜(Cu)、铁(Fe)与空腹血糖(FBS)的相关性,并研究2型糖尿病患者与对照组铁氧化酶、转铁蛋白、总铁结合能力的比较。本研究共纳入40名受试者,将其分为两组(对照组和糖尿病患者)。从所有参与者身上采集空腹血液样本,分析Cr、Mg、Zn、Cu和Fe的水平。采用电感耦合等离子体发射光谱法测定各样品血清微量元素浓度。2型糖尿病合并FBS患者血清Mg、Zn、Fe水平呈负相关,血清铜水平与FBS呈显著正相关,血清铬水平与FBS无显著相关。T2DM患者铁氧化酶、总结合能力显著升高,转铁蛋白显著降低。
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引用次数: 1
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Al-Qadisiyah Journal Of Pure Science
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