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Investigation of Virulence Factors in Microbial Organisms that Associated with Public Health Risk Isolates from Different Environmental Regions 不同环境区与公共卫生风险相关的微生物中毒力因子的研究
Pub Date : 2023-02-25 DOI: 10.23851/mjs.v33i5.1303
S. A. Abid, Sarah N. Aziz, N. A. Saeed, S. N. Mizil, I. AL-Kadmy, N. Hussein, Nadal A. Al-Saryi, S. Ibrahim, J. D. Hussein
Infectious diseases caused by infected tools in the environments are threaten to the safety and public health. Transmission sources of these infectious diseases are unknown, but it is thought that non-living materials called fomites, are the major source of acquired infections. Three hundred and one swabs were taken from different sources and cultured on blood agar to study heamolysis ability of isolated bacteria. In this study, MacConkey agar was used to isolate Gram-negative bacteria and Sabouraud agar (SDA) to isolate fungi. The biofilm formation test was done by Congo red plate assay. 41 (13.6%) bacterial isolates were obtained and (18.27%) of fungi were isolated on Sabouraud agar (SDA). Staphylococcus aureus was the more frequent bacterial species that isolated in this study. 29% of samples showed hemolysin activity on blood agar and 32%of the isolates were biofilm- producer. Results revealed that (7.9%) of Gram-negative bacteria harbored the fimH gene, (9%) harbored the icaA were Gram-positive and 6.3 % of fungal samples had HWP1 gene. Furthermore, (9.3%) from the total samples are bacterial samples harbored hla gene belong to Staphylococcus spp. Furthermore, (5.07%) of tested samples possessed hlyA gene were Gram-negative bacteria. We found in our study that infectious organisms can be transmitted from one individual to another by fomites responsible for acquired infection.
环境中受感染工具引起的传染病威胁着安全和公众健康。这些传染病的传播来源尚不清楚,但人们认为,被称为污染物的非生物物质是获得性感染的主要来源。从不同来源取拭子301份,在血琼脂上培养,研究分离细菌的溶血能力。本研究采用MacConkey琼脂分离革兰氏阴性菌,Sabouraud琼脂(SDA)分离真菌。生物膜形成试验采用刚果红平板法。在Sabouraud琼脂(SDA)上分离到细菌41株(13.6%),真菌41株(18.27%)。金黄色葡萄球菌是本研究中分离出的较常见的细菌种类。29%的菌株对血琼脂具有溶血素活性,32%的菌株能产生生物膜。结果表明,革兰氏阴性菌(7.9%)携带fimH基因,革兰氏阳性菌(9%)携带icaA基因,革兰氏阳性菌(6.3%)携带HWP1基因。携带hla基因的细菌样本中有9.3%为葡萄球菌,携带hlyA基因的细菌样本中有5.07%为革兰氏阴性菌。我们在研究中发现,传染性生物体可以通过引起获得性感染的污染物从一个个体传播到另一个个体。
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引用次数: 4
The Influence of Age, Gender and Weight on Sex Hormone Levels and Histological Development of Reproductive Organs in Arabi Sheep 年龄、性别和体重对阿拉伯羊性激素水平和生殖器官组织发育的影响
Pub Date : 2023-02-25 DOI: 10.23851/mjs.v33i5.1309
Aseel Jameel Jumaa, W. Y. Kassim
This study was conducted in the College of Agriculture - University of Basra from 1/9/2020 to 15/1/2021 by collecting 120 blood samples from males and females of the Arabi sheep breed, as well as taking samples from reproductive organs (testes and ovaries) to evaluate the effect of age, weight and sex of animals on sexual hormone levels . Samples were divided into three age groups (5-8 months, 9-12 months and 1-1.5 years) and three body weight groups (15-25, 26-36, 37-47) kg. The results showed a significant (P<0.05) increase in the concentration of gonadotropin hormones (FSH, LH) and steroidal hormones (estrogen, progesterone and testosterone) in animals older than 9 months. The concentration of FSH and LH hormones were higher females compared to males. All levels of sex hormones significantly (P<0.05) increased when the body weight increased to more than 26 kg in both sexes. Reproductive organs in both sexes (testes and ovaries) developed when the animal age and weight increased.
本研究于2020年9月1日至2021年1月15日在巴士拉大学农业学院进行,采集了120只阿拉比羊雄性和雌性的血液样本,并采集了生殖器官(睾丸和卵巢)样本,以评估动物年龄、体重和性别对性激素水平的影响。样本分为3个年龄组(5-8月龄、9-12月龄和1-1.5岁)和3个体重组(15-25、26-36、37-47)kg。结果显示,9月龄以上动物的促性腺激素(FSH、LH)和甾体激素(雌激素、孕酮和睾酮)浓度显著(P<0.05)升高。卵泡刺激素和黄体生成素浓度女性高于男性。当体重增加到26 kg以上时,两性性激素水平均显著升高(P<0.05)。雌雄生殖器官(睾丸和卵巢)随着动物年龄和体重的增加而发育。
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引用次数: 0
Detection of Biofilm Formation in Classical and Hypervirulent Klebsiella pneumoniae 经典和高致病性肺炎克雷伯菌生物膜形成的检测
Pub Date : 2023-02-25 DOI: 10.23851/mjs.v33i5.1315
Iman Mohammad Mahmood Alansary, Nadal A. Al-Saryi
Klebsiella pneumoniae has considered as a relevant healthcare-associated pathogen, its risk of infections is increasing in the presence of medical devices. K. pneumoniae is known for its ability to form biofilm on biotic and abiotic surfaces. Biofilm of K. pneumoniae assists in bacterial protection from host immune responses and antibiotics. Hypervirulent Klebsiella pneumoniae (hvKp) emerges as a new pathotype, which first appeared in Asian Pacific Rim but spread globally. Thus, this study aimed to investigate the ability of K. pneumoniae including hvKp and potential hvKp isolates to form biofilm. One hundred isolates of K. pneumoniae were collected from different hospitals in Baghdad city. These isolates were identified by phenotypic characterization on selective agar plates, biochemical tests, VITEK II, and molecular identification. Biofilm formation was tested in these isolates by two methods, congo red and Tissue Culture Plate method. In congo red method, 33% of the isolates were biofilm producer and (63%) can form biofilm by TCP method divided as: 14% strong, 15 moderate, 34% weak, and 37% non-biofilm producer. The hvKp and potential hvKp isolates showed a variable ability to form biofilm as classical K. pneumoniae.
肺炎克雷伯菌被认为是一种与卫生保健相关的病原体,在医疗器械的存在下,其感染风险正在增加。肺炎克雷伯菌以其在生物和非生物表面形成生物膜的能力而闻名。肺炎克雷伯菌的生物膜有助于细菌免受宿主免疫反应和抗生素的侵害。高致病性肺炎克雷伯菌(hvKp)作为一种新的病原体出现,它最初出现在亚太地区,但在全球范围内传播。因此,本研究旨在探讨肺炎克雷伯菌包括hvKp和潜在hvKp分离株形成生物膜的能力。从巴格达市不同医院收集了100株肺炎克雷伯菌分离株。这些分离物通过选择琼脂板上的表型表征、生化试验、VITEK II和分子鉴定进行鉴定。采用刚果红法和组织培养平板法检测分离菌株的生物膜形成情况。在刚果红法中,33%的分离菌能形成生物膜,63%的分离菌能形成生物膜,按TCP法分为:强分离菌14%,中等分离菌15株,弱分离菌34%,不形成生物膜的分离菌37%。hvKp和潜在hvKp分离株表现出与典型肺炎克雷伯菌形成生物膜的不同能力。
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引用次数: 1
The Relation Between the Noise Stress Effects and Neurotransmitters, Sex Hormones Levels in the Male and Female Balb/c Mice 噪声应激效应与雌雄Balb/c小鼠神经递质、性激素水平的关系
Pub Date : 2023-02-25 DOI: 10.23851/mjs.v33i5.1321
Zainab A. A. Al-Shammary, Sabah A. Hameid, J. Jouda
Many studies suggest that noise stress could damage many body systems such as nervous and endocrine systems, so the goal of this study was to demonstrate how the levels of neurotransmitters and sex hormones in mature male and female mice are affected by different noise stress exposure durations? In this study, 60 Balb/C mice (males and females) were used. Fifty mice were exposed to 90dB noise stress for 4h/daily and were scarified in different time points; 1,7,14,21, and 28 days while the rest 10mice were not exposed to noise stress and they serve as control. After the end of the experiment, the blood was collected and the serum samples were used to determine neurotransmitters levels, serotonin (5-HT) and noradrenaline (NA), and hormones levels included testosterone, estrogen and cortisol levels. The findings showed that neurotransmitters levels were significantly higher in mice exposed to noise stress for 1,8,14, and 21days which was non-significant decrease compared to the control in the 28-day stress group. While the levels of cortisone were continuously rising in the exposed noise stress mice groups with the length of duration, the levels of estrogen in female and testosterone in male were continuously decreasing. Interestingly, the levels of neurotransmitters and cortisol were significantly higher in females than males in all noise stress groups. Additionally, these effects completely differ after 28 days of noise exposure, which needs more future studies to prove the risk of exposure to this type of stress on the body systems.
许多研究表明噪音压力会损害许多身体系统,如神经系统和内分泌系统,因此本研究的目的是证明成熟的雄性和雌性小鼠的神经递质和性激素水平是如何受到不同噪音压力暴露时间的影响的。本研究选用Balb/C小鼠(雄性和雌性)60只。将50只小鼠置于90dB噪声环境下,每天4小时,在不同时间点进行损伤;第1、7、14、21和28天,其余10只小鼠不受噪声胁迫,作为对照组。实验结束后采血,测定血清中神经递质、血清中5-HT、去甲肾上腺素(NA)、睾酮、雌激素、皮质醇等激素水平。结果表明,噪声应激1、8、14和21天小鼠的神经递质水平显著升高,与28天应激组相比无显著降低。暴露噪声应激小鼠组可的松水平随着持续时间的延长而不断升高,雌性雌激素和雄性睾酮水平则不断下降。有趣的是,在所有噪音压力组中,女性的神经递质和皮质醇水平明显高于男性。此外,这些影响在噪音暴露28天后完全不同,这需要更多的未来研究来证明暴露于这种类型的压力对身体系统的风险。
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引用次数: 0
Influence of Graphene Sheets Accumulation on Optical Band Gap Enhanced Graphite Exfoliation 石墨烯片积累对光学带隙增强石墨剥离的影响
Pub Date : 2022-12-30 DOI: 10.23851/mjs.v33i4.1216
Riad M. Hameed, Ahmad Al-Haddad, Abbas K. H. Albarazanchi
Recently, graphene has been adopted to replace other expansive materials in various devices that perform numerous functionalities in many industrial fields. Meanwhile, researchers are still investigating the amazing properties of graphene. Herein, reduced graphene oxide (rGO) has been successfully exfoliated directly using a graphite rod in a modified electrolyte including a table salt as a co-electrolyte. The structure of graphene obtained by using exfoliation methods shows a low ratio of O/C and confirms the high crystallinity of rGO. The thickness of rGO was adjusted during the drying of the drops of rGO solution and obtained about an 8-80 nm rGO thick. The increased O/C ratio and crystallinity enhancement could be attributed to the quantum confinement effect. Further investigations to estimate the decay constant of the optical band gap during the thinning of the rGO layers show that the optical band gap was associated with thicknesses of the rGO at a decay constant of 0.3367±0.00205. These results would be crucial in several optical applications that depend on the thicknesses and the band gap.
近年来,石墨烯已被用于取代其他膨胀材料的各种器件,在许多工业领域中执行许多功能。与此同时,研究人员仍在研究石墨烯的惊人特性。本文中,还原氧化石墨烯(rGO)已经成功地在改性电解质中使用石墨棒直接剥离,其中包括食盐作为共电解质。用剥离法得到的石墨烯结构显示出低的O/C比,证实了还原氧化石墨烯的高结晶度。在还原氧化石墨烯溶液滴液干燥过程中调整还原氧化石墨烯的厚度,得到的还原氧化石墨烯厚度约为8 ~ 80 nm。O/C比的增加和结晶度的增强可归因于量子约束效应。进一步的研究估计了在rGO层变薄过程中光学带隙的衰减常数,结果表明,光学带隙与rGO厚度相关,衰减常数为0.3367±0.00205。这些结果在一些依赖于厚度和带隙的光学应用中是至关重要的。
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引用次数: 1
Effect of Colloidal Cu NPs/Cinnamon Extract on the Antibacterial Activity 胶体铜NPs/肉桂提取物对抗菌活性的影响
Pub Date : 2022-12-30 DOI: 10.23851/mjs.v33i4.1154
Maryam Mohammed K., B. M. Ahmed
This study explores a new facile method of obtaining plant extract using a plasma jet and a way of producing Cu nanoparticles (Cu NPs) using pulsed laser ablation in liquid. A 532 nm Nd:YAG laser at varying energies (500, 700, and 900 mJ) was used to prepare Cu NPs. Cinnamon bark extract was prepared quickly by DC plasma discharge (plasma jet) exposure at 5 and 15 min. The study showed the effects of a mixture (Cu NPs with cinnamon extract) on Escherichia coli bacteria. The colloids inhibited E. coli; the inhibition increased with duration and laser energy. The characterization of the Cu NPs was performed using ultraviolet-visible spectrophotometry, and field emission-scanning electron microscopy (FE-SEM).
本研究探索了一种利用等离子体射流获取植物提取物的简便方法和一种在液体中利用脉冲激光烧蚀制备铜纳米粒子的方法。利用532 nm不同能量(500、700和900 mJ)的Nd:YAG激光器制备Cu NPs。采用直流等离子体放电(等离子体射流)在5和15 min下快速制备肉桂皮提取物。研究了铜纳米粒子与肉桂提取物的混合物对大肠杆菌的影响。胶体抑制大肠杆菌;抑制作用随持续时间和激光能量的增加而增加。采用紫外可见分光光度法和场发射扫描电镜(FE-SEM)对铜纳米粒子进行了表征。
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引用次数: 1
Study IR- Raman Spectra properties of Aluminium Phosphide Diamondoids Nanostructures via DFT 利用DFT研究磷化铝类金刚石纳米结构的红外-拉曼光谱特性
Pub Date : 2022-12-30 DOI: 10.23851/mjs.v33i4.1182
H. A. Fayyadh, Dh. Kh. Kafi, A. Darweesh
Density Functional Theory has been utilized to investigate the electronic and structural characteristics of Aluminium phosphide (AlP). The exchange-correlation potential was calculated using the Generalized Gradient Approximation. The structural, electronic and vibrational features of AlP diamondoids and nanocrystals were investigated using Density Functional Theory at the PBE/6-31(d) level, which included polarization functions. Vibrational modes have been optimized concerning IR intensity, force constants, and lowered masses. In this study there are two components to the vibrational force constant for AlP diamondoids. The first one is distinguished by a reduced mass that is greater than 1 amu and consists primarily of Al-P vibrations that are positioned roughly between 0 and 231 cm-1. The second component has a decreased mass very near to 1 amu and is in the 1228–2400 cm–1 range. It is entirely made up of hydrogen vibrational modes. AlP diamondoids were evaluated with the results of experimental bulk in terms of molecular size-related changes in allocated vibrational frequencies.
利用密度泛函理论研究了磷化铝(AlP)的电子和结构特性。利用广义梯度近似计算交换相关势。利用PBE/6-31(d)水平的密度泛函理论(包括极化函数)研究了AlP类金刚石和纳米晶体的结构、电子和振动特征。振动模式在红外强度、力常数和降低质量方面进行了优化。在本研究中,AlP类金刚石的振动力常数有两个分量。第一个的特点是质量减小,大于1amu,主要由Al-P振动组成,其位置大致在0到231 cm-1之间。第二个分量的质量减小到非常接近1 μ m,在1228-2400 cm-1范围内。它完全由氢的振动模式组成。用实验体积的结果来评估AlP类金刚石在分配振动频率方面的分子大小相关变化。
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引用次数: 0
Study of Phenotypic and Genotypic Factors of Staphylococcus aureus Clinical Local Isolates 金黄色葡萄球菌临床局部分离株表型和基因型因素的研究
Pub Date : 2022-12-30 DOI: 10.23851/mjs.v33i4.1166
A. M. Ali, M. M. Abdallah
The isolates of Staphylococcus aureus were isolated from patients with various infections in hospitals, the isolates were identified and accurately diagnosed by phenotypic examination and biochemical tests, as well Vitek-2, and then genetic detection and diagnosis of many of the pathogenic factors associated with Staphylococcus aureus using conventional polymerase chain reaction (PCR) and testing for association by antibiotic resistance and production of some toxins by Staphylococcus aureus. After performing analysis of statistical, it was set up that the correlation coefficient of the PCR technique using virulence genes, sensitivity test to antibiotics and other virulence factors were significant at p < 0.05, but was insignificant with the biofilm production.
从医院各类感染患者中分离金黄色葡萄球菌,通过表型检查、生化检测、Vitek-2等方法对分离株进行鉴定和准确诊断,然后采用常规聚合酶链式反应(PCR)、检测金黄色葡萄球菌耐药性和部分毒素产生的相关性,对金黄色葡萄球菌的多种相关致病因素进行基因检测和诊断。经统计分析发现,毒力基因PCR技术与抗生素敏感性试验及其他毒力因子的相关系数均显著(p < 0.05),但与生物膜产量的相关系数不显著。
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引用次数: 0
Special Arcs in PG(3,13) PG(3,13)中的特殊弧线
Pub Date : 2022-12-30 DOI: 10.23851/mjs.v33i4.1197
Saja Makki Attook, E. B. Al-Zangana
In this paper, we constructed new arcs in the three-dimensional projective space over GF(13) of degrees 2, 3, 4, 5, 6, 7, 10, 11, 14, 15, 18, 19, 23, 28, 30, 47, 55, 98 and sizes 2, 4, 5, 7, 10, 14, 17, 20, 28, 34, 35, 68, 70, 85, 119, 140, 170, 238, 340, 476, 595, 1190. These arcs are classified to complete and incomplete arcs. Also, the incomplete arcs are extended to complete arcs.
本文在GF(13)上的三维射影空间中构造了新的圆弧,圆弧的角度为2、3、4、5、6、7、10、11、14、15、18、19、23、28、30、47、55、98,尺寸为2、4、5、7、10、14、17、20、28、34、35、68、70、85、119、140、170、238、340、476、595、1190。这些弧线分为完全弧线和不完全弧线。同时,将不完全弧扩展为完全弧。
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引用次数: 0
Numerical Simulation of The Spectroscopic Properties of The Laser Dye for Low Concentrations 低浓度激光染料光谱特性的数值模拟
Pub Date : 2022-12-30 DOI: 10.23851/mjs.v33i4.1158
H. Dagher
In the current study, a mathematical model using the MATLAB program was studied depending on the logistic power peak function to simulate the change in the absorbance with the different values of the concentrations of Rhodamine B. There are five parameters that have been applied to the concentration values of this laser dye that are proposed by the logistic power peak function of the seventh degree. To measure the absorption measurements, a range of concentrations from 4.7x10-7 to 3.76x10-6 M was prepared from Rhodamine B and dissolved in deionized water with a peak absorption at 556 nm. A JASCO V-630 spectrophotometer was used to record the spectra of the dye. The intensity is detected using a silicon photodiode (S1337). The results were found that the suggested function approved the relationship between the absorbance and the values of the concentrations with a significant accuracy depending on the high values of the correlation coefficients between the approximated original data which were determined more than 99.2%. The advantages of this function represent by providing parameters, which are used to describe the mathematical processes with high-resolution property profiles.
在目前的研究中,利用MATLAB程序研究了依赖logistic功率峰值函数的数学模型,以模拟罗丹明b浓度不同值时吸光度的变化。该激光染料的浓度值有五个参数被应用,这些参数是由七次logistic功率峰值函数提出的。为了测量吸收测量,从罗丹明B制备了4.7x10-7到3.76x10-6 M的浓度范围,并溶解在去离子水中,吸收峰在556 nm处。用JASCO V-630分光光度计记录染料的光谱。使用硅光电二极管(S1337)检测强度。结果表明,该函数能很好地反映吸光度与浓度之间的关系,其准确度取决于近似原始数据之间的相关系数的高值,确定的相关系数大于99.2%。该函数的优点在于提供参数,这些参数用于描述具有高分辨率属性配置文件的数学过程。
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引用次数: 0
期刊
Al-Mustansiriyah Journal of Science
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