Annales de biologie clinique最新文献

Purpose: Measurement of the haemolysis index (HI) is usually performed in clinical chemistry laboratories in order to inform about whether biological analyses are influenced by in vivo or in vitro haemolysis of the specimen. Our aim was to evaluate the analytical performance of Abbott C-16000 analyser HI measurement in order to determine whether this could be used to reliably measure cell-free haemoglobin (fHB) in plasma samples.

Methods: The repeatability, reproducibility, lower limit of detection (LLOD) and lower limit of quantification (LLOQ) of C-16000 HI measurement were determined as well as the potential interference of bilirubin, triglycerides and myoglobin. C-16000 HI values of biological samples with various ranges of fHB were compared to those measured using the established reference method, second-derivate spectroscopy.

Results: Results: C-16000 HI determination showed excellent linear correlation with the reference method (y = 1.0043x – 1.248, R² = 0.998), a broad analytical measurement range (400-20,000 mg/L; y = 0.9904x + 72.972, R² = 0.999), clinically relevant LLOD (56 mg/L) and LLOQ (84 mg/L), good repeatability (coefficient of variation (CV) = 1-15%) and good reproducibility (CV = 5-7%). No interference was observed with myoglobin at concentrations as high as 35,447 mg/L, unconjugated and conjugated bilirubin (at concentrations up to 500 mg/L and 375 mg/L, respectively) or triglycerides up to 6.8 mmol/L. However, a significant underestimation of fHB concentrations was observed at higher triglyceride levels.

Conclusion: This study demonstrates that Abbott C-16000 analyser HI is reliable and accurately measures plasma fHB concentrations under pathophysiological conditions except when there are high blood concentrations of triglycerides.

Since 2010, the postgraduate training diploma in laboratory medicine has experienced a strong decline in its attractiveness to French medical students. This work aims to objectify and quantify this decline between 2004 and 2021. To do so, we have carried out an analyze of the trend to choose laboratory medicine after the national ranking review performed by all French students. Then, we have also compiled the data on the right to change one’s mind and final quits from laboratory medicine postgraduate training. The laboratory medicine reform has led to deep changes in its organization. The accreditation requirements, the laboratories grouping, as well as the financialization of this activity have strongly changed the work of laboratory physician and impacted its attractiveness for the young generations.

Nosocomial infections constitute a significant public health problem but are poorly controlled in our health structures, especially those associated with resuscitation care. The first objective of this study was to identify the different microbial strains present in different biological samples taken from patients staying in the resuscitation unit of the Annaba University Hospital Center. The second objective was to assess the antimicrobial sensitivity of isolated microbes from the patients’ samples, to determine the risk factors, the most incriminated microbial agents in nosocomial infections. During the study period from January 2013 to December 2016, we collected 1,151 biological samples from 1,938 patients admitted to Resuscitation Medical Service. The samples were subjected to different microbiological analyses. Our results showed that over 59% of the collected samples were microbiologically positive. The identified species include Candida albicans (115 cases) and Candida.sp (81 cases). The Gram-negative bacterial strains found in the samples included Acinetobacter baumannii (108 cases), Klebssiella pneumoniae (99 cases) Pseudomonas aeruginosa (79 cases), and Escherichia coli (73 cases). Gram positive bacteria included Staphylococcus aureus (94 cases) and Enterococcus faecalis (53 cases). The antibiogram analyses showed significant antibiotic resistance reaching 93.75% for ampicillin, but sensitivity to colistin reaching 81.81%. Moreover, the fungal strains are represented by the genus albicans, showing a significant resistance to antifungals, reaching 80% with miconazole. Conclusion. The nosocomial infections in the medical unit were caused by the candida genus and multi-resistant bacteria to various antibiotics and antifungals. The most important factor associated with these infections was the use of medical devices.

Background: Point of care testing (POCT) tests are needed to assess severity and to help for triage in hospital and in prehospital settings. Before their use, the analytical performances of POCTs have to be compared with central laboratory reference methods. In this study, we describe the comparability of results obtained by either the Abbott i-STAT® System POCT handheld device or the blood gases analyzer of the central laboratory of our hospital.

Methods: Sample blood from 37 septic patients admitted to the intensive care unit (ICU) were assayed by Abbott i-STAT® System POCT and Radiometer ABL800 Flex® lab analyzer. Studied parameters were as follows: pH, pO2, pCO2, base excess (BE), HCO3- and lactate. Comparability was evaluated using Bland-Altman method. The clinical value for possible mismatch issued of values differences was also assessed.

Results: Quite acceptable correlations in results of POCT and laboratory analyzer were observed with R² most of time above 0.85. Bland-Altman analysis showed a bias of 1.26% for Abbott i-STAT® System POCT vs laboratory.

Conclusion: Abbott i-STAT® System POCT handheld device is comparable to Radiometer ABL800 Flex® lab analyzer and concordant with laboratory analysis. Abbott i-STAT® System POCT handled device could be used in the prehospital settings in order to evaluate the severity of sepsis.

The Cycle Threshold (Ct) value of SARS-CoV-2 RT-PCR are used as an indicator of viral load. Using a collection of 45 fresh nasopharyngeal samples collected on universal transport media, we compare the Ct value obtained with 2 RT-PCR assays, the Alinity M SARS-CoV-2 and the Alinity M RESP-4-Plex (Abbott Molecular, Des Plaines, Illinois, Etats-Unis) processed on an Alinity M device. The assays are highly correlated; however, the Ct values were in median lower of 4.54 with the Alinity M RESP-4-Plex. This difference could be attributed to earlier detection of positivity by the software of the Alinity M rather than a difference in RT-PCR performances. The Ct-value of SARS-CoV-2 RT-PCR should be interpreted with caution taking into account the clinical context, pre-analytical and analytical findings.