Annales immunologiae Hungaricae最新文献

Sera administered intravenously to mice hypersensitized with normal horse serum and with horse serum of anti-lymphocytic activity, produce reactions that differ considerably in mice treated in different ways. The experiments of the authors indicate that the anaphylactogenic effect of ATS is substantially stronger than that or normal horse serum. The paper discusses the clinical and theoretical conclusions to be drawn from this phenomenon observed in the experiments.

Mice with their lymphoid system made atrophic by neonatal thymectomy, showed increased sensitivity to DAD a lymphotropic cytostatic agent. Wasting syndrome developed earlier and in a higher per cent of neonatally thymectomized mice, when the animals were treated also with the subethal dose of DAD.

An immunodiffusion method combined with thin-layer chromathography was elaborated for the assessment of the molecular weight of IgM. Sera from 100 normal individuals as well as from 162 patients with different types of paraproteinaemia, 100 patients with uveitis and 55 venous umbilical cord sera were analysed. Presence of 75 IgM, besides the usual 19S IgM was detected in 21% of the normal sera, in 27% of the paraproteinaemic sera, in 30% of the uveitic sera and in 5.4% of venous umbilical cord sera. IgM level in the sera was determined by radial immunodiffusion. The authors didn't find correlation between the presence of 7S IgM and different levels of IgM.

Normal human pooled plasma was fractionated by a variety of methods. The IgE concentration of the different fractions was determined by a solid-phase radioimmunoassay. The results of these studies indicate, that polyclonal IgE behaves similarly to IgE of myeloma origin. A biospecific method was worked out to purify IgE from fraction III of the cold ethanol fractionation procedure.

The authors have compared the anaphylaxis due to active and passive sensitization of mice. In the case of active sensitizing, anti-mouse anti-thymocyte horse serum (ATS), and/or normal horse serum (NHS), whereas in the case of passive sensitizing, plasma, peripheral leukocytes, spleen cells and thymocytes of sensitized animals were used. Provocation of shock was carried out by intravenous administration of ATS or NHS. Irreversible anaphylaxis occurred in a significantly higher rate in the case of ATS than NHS sensitivity, produced either actively, or passively. Differences have been found also between the 4 investigated materials according to their passive transfer potency.

The role of the immunizing dose on antibody production was studied in rabbits. The humoral immune response to soluble and insoluble human IgG was evaluated by the measurement of the amount of specific antibodies. The immobilizations of antigen was made by antigen-antibody precipitation and attachment of the protein to polyacrylamide and agarose matrices. It was found, that the antibody production did not decrease when the antigen dose was reduced from 100 microng to 300 microng; application of the antigen in immobilized form did not enhance the antibody response.

The authors describe a simple method for isolating heterogeneous IgM from fraction I obtained as a by-product of the large scale fractionation of human plasma. The optimum circumstances of fractionation were determined and it was found that the majority of IgM is precipitated between 2 and 5% polyethylene glycol concentration and 1.1 and 1.8 M ammonium sulphate concentration. After a simple fractionation and gel filtration, a preparation of high purity can be obtained.

The authors investigated serum samples of 5000 inhabitants (age 0 to 85 years) in a crowded industrial area, by means of the counterimmunoelectrophoretic method. They evaluate their results in relation of age, sex and occupation and presented literary data about the subtypes of the carriers in Budapest. They compared them with data of Hungarian and several other authors respectively. Some possible reasons of the differences are discussed and the importance of the non-parental transmission is emphasized.

The authors have carried out various tests for determining protein concentrations. They have compared the methods, and have found, as well as proved, that the spectrophotometric method I of protein determination can be employed with sufficient accuracy for the purpose of serial tests.