There are different stress resistance among different breeds of pigs. Changes in intestinal stem cells (ISCs) are still unclear among various breeds of piglets after early weaning. In the current study, Taoyuan Black and Duroc piglets were slaughtered at 21 days of age (early weaning day) and 24 days of age (3 days after early weaning) for 10 piglets in each group. The results showed that the rate of ISC-driven epithelial renewal in local Taoyuan Black pigs hardly changed after weaning for 3 days. However, weaning stress significantly reduced the weight of the duodenum and jejunum in Duroc piglets. Meanwhile, the jejunal villus height, tight junction-related proteins (ZO-1, Occludin, and Claudin1), as well as the trans-epithelial electrical resistance (TEER) values, were down-regulated after weaning for 3 days in Duroc piglets. Moreover, compared with Unweaned Duroc piglets, the numbers of Olfm4+ ISC cells, PCNA+ mitotic cells, SOX9+ secretory progenitor cells, and Villin+ absorptive cells in the jejunum were reduced significantly 3 days after weaning. And ex vivo jejunal crypt-derived organoids exhibited growth disadvantages in weaned Duroc piglets. Notably, the Keap1/Nrf2 signaling activities and the expression of HO-1 were significantly depressed in weaned Duroc piglets compared to Unweaned Duroc piglets. Thus, we can conclude that ISCs of Duroc piglets were more sensitive to weaning stress injury than Taoyuan Black piglets, and Keap1/Nrf2 signaling is involved in this process.
{"title":"Early Weaning Inhibits Intestinal Stem Cell Expansion to Disrupt the Intestinal Integrity of Duroc Piglets via Regulating the Keap1/Nrf2 Signaling.","authors":"Ying-Chao Qin, Cheng-Long Jin, Ting-Cai Hu, Jia-Yi Zhou, Xiao-Fan Wang, Xiu-Qi Wang, Xiang-Feng Kong, Hui-Chao Yan","doi":"10.3390/antiox13101188","DOIUrl":"10.3390/antiox13101188","url":null,"abstract":"<p><p>There are different stress resistance among different breeds of pigs. Changes in intestinal stem cells (ISCs) are still unclear among various breeds of piglets after early weaning. In the current study, Taoyuan Black and Duroc piglets were slaughtered at 21 days of age (early weaning day) and 24 days of age (3 days after early weaning) for 10 piglets in each group. The results showed that the rate of ISC-driven epithelial renewal in local Taoyuan Black pigs hardly changed after weaning for 3 days. However, weaning stress significantly reduced the weight of the duodenum and jejunum in Duroc piglets. Meanwhile, the jejunal villus height, tight junction-related proteins (ZO-1, Occludin, and Claudin1), as well as the trans-epithelial electrical resistance (TEER) values, were down-regulated after weaning for 3 days in Duroc piglets. Moreover, compared with Unweaned Duroc piglets, the numbers of Olfm4<sup>+</sup> ISC cells, PCNA<sup>+</sup> mitotic cells, SOX9<sup>+</sup> secretory progenitor cells, and Villin<sup>+</sup> absorptive cells in the jejunum were reduced significantly 3 days after weaning. And ex vivo jejunal crypt-derived organoids exhibited growth disadvantages in weaned Duroc piglets. Notably, the Keap1/Nrf2 signaling activities and the expression of HO-1 were significantly depressed in weaned Duroc piglets compared to Unweaned Duroc piglets. Thus, we can conclude that ISCs of Duroc piglets were more sensitive to weaning stress injury than Taoyuan Black piglets, and Keap1/Nrf2 signaling is involved in this process.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":null,"pages":null},"PeriodicalIF":6.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11505184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Polycystic kidney disease (PKD) is a rare but significant renal condition with major implications for global acute and chronic patient care. Oxidative stress and reactive oxygen species (ROS) can significantly alter its pathophysiology, clinical outcomes, and treatment, contributing to negative outcomes, including hypertension, chronic kidney disease, and kidney failure. Inflammation from ROS and existing cysts propagate the generation and accumulation of ROS, exacerbating kidney injury, pro-fibrotic signaling cascades, and interstitial fibrosis. Early identification and prevention of oxidative stress and ROS can contribute to reduced cystic kidney disease progression and improved longitudinal patient outcomes. Increased research regarding biomarkers, the pathophysiology of oxidative stress, and novel therapeutic interventions alongside the creation of comprehensive guidelines establishing methods of assessment, monitoring, and intervention for oxidative stress in cystic kidney disease patients is imperative to standardize clinical practice and improve patient outcomes. The integration of artificial intelligence (AI), genetic editing, and genome sequencing could further improve the early detection and management of cystic kidney disease and mitigate adverse patient outcomes. In this review, we aim to comprehensively assess the multifactorial role of ROS in cystic kidney disease, analyzing its pathophysiology, clinical outcomes, treatment interventions, clinical trials, animal models, and future directions for patient care.
{"title":"Reactive Oxygen Species in Cystic Kidney Disease.","authors":"Sanat Subhash, Sonya Vijayvargiya, Aetan Parmar, Jazlyn Sandhu, Jabrina Simmons, Rupesh Raina","doi":"10.3390/antiox13101186","DOIUrl":"10.3390/antiox13101186","url":null,"abstract":"<p><p>Polycystic kidney disease (PKD) is a rare but significant renal condition with major implications for global acute and chronic patient care. Oxidative stress and reactive oxygen species (ROS) can significantly alter its pathophysiology, clinical outcomes, and treatment, contributing to negative outcomes, including hypertension, chronic kidney disease, and kidney failure. Inflammation from ROS and existing cysts propagate the generation and accumulation of ROS, exacerbating kidney injury, pro-fibrotic signaling cascades, and interstitial fibrosis. Early identification and prevention of oxidative stress and ROS can contribute to reduced cystic kidney disease progression and improved longitudinal patient outcomes. Increased research regarding biomarkers, the pathophysiology of oxidative stress, and novel therapeutic interventions alongside the creation of comprehensive guidelines establishing methods of assessment, monitoring, and intervention for oxidative stress in cystic kidney disease patients is imperative to standardize clinical practice and improve patient outcomes. The integration of artificial intelligence (AI), genetic editing, and genome sequencing could further improve the early detection and management of cystic kidney disease and mitigate adverse patient outcomes. In this review, we aim to comprehensively assess the multifactorial role of ROS in cystic kidney disease, analyzing its pathophysiology, clinical outcomes, treatment interventions, clinical trials, animal models, and future directions for patient care.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":null,"pages":null},"PeriodicalIF":6.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11504974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ioana Andreea Barbu, Vlad Alexandru Toma, Augustin Cătălin Moț, Ana-Maria Vlase, Anca Butiuc-Keul, Marcel Pârvu
Medicinal plants are a valuable reservoir of novel pharmacologically active compounds. ROS and free radicals are primary contributors to oxidative stress, a condition associated with the onset of degenerative diseases such as cancer, coronary heart disease, and vascular disease. In this study, we used different spectrophotometry methods to demonstrate the antioxidant properties of 6 Allium extracts: Allium fistulosum; Allium ursinum; Allium cepa: Arieș red cultivar of A. cepa, and white variety of A. cepa; Allium sativum; and Allium senescens subsp. montanum. HPLC-MS determined the chemical composition of the extracts. Among the tested extracts, the Arieș red cultivar of A. cepa stands out as having the best antioxidant activity, probably due to the high content of polyphenols and alliin (12.67 µg/mL and 3565 ng/mL, respectively). The results obtained in this study show that Allium extracts have antioxidant activity, but also free radical scavenging capabilities. Also, their interactions with cytochrome c and hemoglobin can be the basis of future studies to create treatments for oxidative stress-related diseases.
药用植物是新型药理活性化合物的宝贵宝库。ROS 和自由基是导致氧化应激的主要因素,而氧化应激与癌症、冠心病和血管疾病等退行性疾病的发生有关。在这项研究中,我们使用了不同的分光光度法来证明 6 种薤白提取物的抗氧化特性:这 6 种薤白提取物分别是:瘘管薤白、乌苏薤白、牛肝菌薤白:牛肝菌薤白红色栽培品种和牛肝菌薤白白色品种、荠菜薤白和莴苣薤白亚种。HPLC-MS 测定了提取物的化学成分。在测试的萃取物中,Arieș red 栽培品种牛肝菌的抗氧化活性最好,这可能是由于多酚和蒜氨酸的含量较高(分别为 12.67 µg/mL 和 3565 ng/mL)。本研究的结果表明,薤白提取物不仅具有抗氧化活性,还具有清除自由基的能力。此外,薤白提取物与细胞色素 c 和血红蛋白之间的相互作用也可作为今后研究氧化应激相关疾病治疗方法的基础。
{"title":"Chemical Composition and Antioxidant Activity of Six <i>Allium</i> Extracts Using Protein-Based Biomimetic Methods.","authors":"Ioana Andreea Barbu, Vlad Alexandru Toma, Augustin Cătălin Moț, Ana-Maria Vlase, Anca Butiuc-Keul, Marcel Pârvu","doi":"10.3390/antiox13101182","DOIUrl":"10.3390/antiox13101182","url":null,"abstract":"<p><p>Medicinal plants are a valuable reservoir of novel pharmacologically active compounds. ROS and free radicals are primary contributors to oxidative stress, a condition associated with the onset of degenerative diseases such as cancer, coronary heart disease, and vascular disease. In this study, we used different spectrophotometry methods to demonstrate the antioxidant properties of 6 <i>Allium</i> extracts: <i>Allium fistulosum</i>; <i>Allium ursinum</i>; <i>Allium cepa</i>: Arieș red cultivar of <i>A. cepa</i>, and white variety of <i>A. cepa</i>; <i>Allium sativum</i>; and <i>Allium senescens</i> subsp. <i>montanum</i>. HPLC-MS determined the chemical composition of the extracts. Among the tested extracts, the Arieș red cultivar of <i>A. cepa</i> stands out as having the best antioxidant activity, probably due to the high content of polyphenols and alliin (12.67 µg/mL and 3565 ng/mL, respectively). The results obtained in this study show that <i>Allium</i> extracts have antioxidant activity, but also free radical scavenging capabilities. Also, their interactions with cytochrome c and hemoglobin can be the basis of future studies to create treatments for oxidative stress-related diseases.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":null,"pages":null},"PeriodicalIF":6.0,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11504208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mª Victoria Peña-García, Mª José Moyano-Gallego, Sara Gómez-Melero, Rafael Molero-Payán, Fernando Rodríguez-Cantalejo, Javier Caballero-Villarraso
Background: Polycyclic aromatic hydrocarbons (PAHs) have toxic potential, especially as carcinogens, neurotoxins, and endocrine disruptors. The objective of this study is to know the impact of exposure to PAHs on the reproductive health of male workers who operate in solar thermal plants.
Methods: Case-control study. A total of 61 men were included: 32 workers exposed to PAH at a solar thermal plant and 29 unexposed people. Seminal quality was studied both at the cellular level (quantity and quality of sperm) and at the biochemical level (magnitudes of oxidative stress in seminal plasma).
Results: In exposure to PAHs, a significantly higher seminal leukocyte infiltration was observed, as well as lower activity in seminal plasma of superoxide dismutase (SOD) and a reduced glutathione/oxidised glutathione (GSH/GSSG) ratio. The oxidative stress parameters of seminal plasma did not show a relationship with sperm cellularity, neither in those exposed nor in those not exposed to PAH.
Conclusion: One year of exposure to PAH in a solar thermal plant does not have a negative impact on the sperm cellularity of the worker, either quantitatively (sperm count) or qualitatively (motility, vitality, morphology, or cellular DNA fragmentation). However, PAH exposure is associated with lower antioxidant capacity and higher leukocyte infiltration in seminal plasma.
背景:多环芳烃(PAHs)具有潜在毒性,尤其是作为致癌物、神经毒素和内分泌干扰物。本研究的目的是了解接触多环芳烃对光热厂男工生殖健康的影响:方法:病例对照研究。方法:病例对照研究:方法:病例对照研究共纳入 61 名男性:32 名接触多环芳烃的太阳能热电厂工人和 29 名未接触者。从细胞水平(精子的数量和质量)和生化水平(精浆中氧化应激的大小)对精液质量进行了研究:结果:在暴露于多环芳烃的情况下,观察到精液中的白细胞浸润明显增加,精浆中超氧化物歧化酶(SOD)的活性和还原型谷胱甘肽/氧化型谷胱甘肽(GSH/GSSG)的比率降低。无论是暴露于多环芳烃还是未暴露于多环芳烃的人群,精浆中的氧化应激参数均未显示出与精子细胞性的关系:结论:在太阳能热电厂工作一年并接触多环芳烃不会对工人的精子细胞数量(精子数量)或质量(活力、生命力、形态或细胞 DNA 片段)产生负面影响。然而,接触多环芳烃会导致抗氧化能力降低和精浆中白细胞浸润增加。
{"title":"One-Year Impact of Occupational Exposure to Polycyclic Aromatic Hydrocarbons on Sperm Quality.","authors":"Mª Victoria Peña-García, Mª José Moyano-Gallego, Sara Gómez-Melero, Rafael Molero-Payán, Fernando Rodríguez-Cantalejo, Javier Caballero-Villarraso","doi":"10.3390/antiox13101181","DOIUrl":"10.3390/antiox13101181","url":null,"abstract":"<p><strong>Background: </strong>Polycyclic aromatic hydrocarbons (PAHs) have toxic potential, especially as carcinogens, neurotoxins, and endocrine disruptors. The objective of this study is to know the impact of exposure to PAHs on the reproductive health of male workers who operate in solar thermal plants.</p><p><strong>Methods: </strong>Case-control study. A total of 61 men were included: 32 workers exposed to PAH at a solar thermal plant and 29 unexposed people. Seminal quality was studied both at the cellular level (quantity and quality of sperm) and at the biochemical level (magnitudes of oxidative stress in seminal plasma).</p><p><strong>Results: </strong>In exposure to PAHs, a significantly higher seminal leukocyte infiltration was observed, as well as lower activity in seminal plasma of superoxide dismutase (SOD) and a reduced glutathione/oxidised glutathione (GSH/GSSG) ratio. The oxidative stress parameters of seminal plasma did not show a relationship with sperm cellularity, neither in those exposed nor in those not exposed to PAH.</p><p><strong>Conclusion: </strong>One year of exposure to PAH in a solar thermal plant does not have a negative impact on the sperm cellularity of the worker, either quantitatively (sperm count) or qualitatively (motility, vitality, morphology, or cellular DNA fragmentation). However, PAH exposure is associated with lower antioxidant capacity and higher leukocyte infiltration in seminal plasma.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":null,"pages":null},"PeriodicalIF":6.0,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11504984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andrea Salica, Vittoria Cammisotto, Raffaele Scaffa, Giulio Folino, Ruggero De Paulis, Roberto Carnevale, Umberto Benedetto, Wael Saade, Antonino Marullo, Sebastiano Sciarretta, Gianmarco Sarto, Silvia Palmerio, Valentina Valenti, Mariangela Peruzzi, Fabio Miraldi, Francesco Giosuè Irace, Giacomo Frati
Background: Oxidative stress and inflammation are typically implied in atherosclerosis pathogenesis and progression, especially in coronary artery disease (CAD). Our objective was to investigate the oxidative stress and inflammation burden directly associated with atherosclerotic plaque in patients with stable coronary disease undergoing coronary artery bypass graft (CABG) surgery. Specifically, markers of oxidative stress and inflammation were compared in blood samples obtained from the atherosclerotic left anterior descending artery (LAD) and blood samples obtained from the healthy left internal thoracic artery (LITA), used as a bypass graft, within the same patient.
Methods: Twenty patients scheduled for off-pump CABG were enrolled. Blood samples were collected from the LITA below anastomosis and the LAD below the stenosis. Samples were analysed for oxidative stress (sNOXdp, H2O2, NO) and inflammation markers (TNFα, IL-6, IL-1β, IL-10).
Results: The analysis showed a significant increase in oxidative stress burden in the LAD as compared to LITA, as indicated by higher sNOX2-dp and H2O2 levels and lower NO levels (p < 0.01). Also, pro-inflammatory cytokines were increased in the LAD as compared to the LITA, as indicated by higher TNFα and IL-6 amounts (p < 0.01). On the other hand, no significant differences could be seen regarding IL-1β and IL-10 levels between the two groups.
Conclusions: The oxidative stress and inflammatory burden are specifically enhanced in the LAD artery of stable coronary patients compared to systemic blood from the LITA of stable coronary patients.
{"title":"Different Oxidative Stress and Inflammation Patterns of Diseased Left Anterior Descending Coronary Artery versus Internal Thoracic Artery.","authors":"Andrea Salica, Vittoria Cammisotto, Raffaele Scaffa, Giulio Folino, Ruggero De Paulis, Roberto Carnevale, Umberto Benedetto, Wael Saade, Antonino Marullo, Sebastiano Sciarretta, Gianmarco Sarto, Silvia Palmerio, Valentina Valenti, Mariangela Peruzzi, Fabio Miraldi, Francesco Giosuè Irace, Giacomo Frati","doi":"10.3390/antiox13101180","DOIUrl":"10.3390/antiox13101180","url":null,"abstract":"<p><strong>Background: </strong>Oxidative stress and inflammation are typically implied in atherosclerosis pathogenesis and progression, especially in coronary artery disease (CAD). Our objective was to investigate the oxidative stress and inflammation burden directly associated with atherosclerotic plaque in patients with stable coronary disease undergoing coronary artery bypass graft (CABG) surgery. Specifically, markers of oxidative stress and inflammation were compared in blood samples obtained from the atherosclerotic left anterior descending artery (LAD) and blood samples obtained from the healthy left internal thoracic artery (LITA), used as a bypass graft, within the same patient.</p><p><strong>Methods: </strong>Twenty patients scheduled for off-pump CABG were enrolled. Blood samples were collected from the LITA below anastomosis and the LAD below the stenosis. Samples were analysed for oxidative stress (sNOXdp, H<sub>2</sub>O<sub>2</sub>, NO) and inflammation markers (TNFα, IL-6, IL-1β, IL-10).</p><p><strong>Results: </strong>The analysis showed a significant increase in oxidative stress burden in the LAD as compared to LITA, as indicated by higher sNOX2-dp and H<sub>2</sub>O<sub>2</sub> levels and lower NO levels (<i>p</i> < 0.01). Also, pro-inflammatory cytokines were increased in the LAD as compared to the LITA, as indicated by higher TNFα and IL-6 amounts (<i>p</i> < 0.01). On the other hand, no significant differences could be seen regarding IL-1β and IL-10 levels between the two groups.</p><p><strong>Conclusions: </strong>The oxidative stress and inflammatory burden are specifically enhanced in the LAD artery of stable coronary patients compared to systemic blood from the LITA of stable coronary patients.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":null,"pages":null},"PeriodicalIF":6.0,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11505158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Serotonin N-acetyltransferase (SNAT) is a pivotal enzyme for melatonin biosynthesis in all living organisms. It catalyzes the conversion of serotonin to N-acetylserotonin (NAS) or 5-methoxytrypytamine (5-MT) to melatonin. In contrast to animal- and plant-specific SNAT genes, a novel clade of archaeal SNAT genes has recently been reported. In this study, we identified homologues of archaeal SNAT genes in ciliates and dinoflagellates, but no animal- or plant-specific SNAT homologues. Archaeal SNAT homologue from the ciliate Stylonychia lemnae was annotated as a putative N-acetyltransferase. To determine whether the putative S. lemnae SNAT (SlSNAT) exhibits SNAT enzyme activity, we chemically synthesized and expressed the full-length SlSNAT coding sequence (CDS) in Escherichia coli, from which the recombinant SlSNAT protein was purified by Ni2+ affinity column chromatography. The recombinant SlSNAT exhibited SNAT enzyme activity toward serotonin (Km = 776 µM) and 5-MT (Km = 246 µM) as substrates. Furthermore, SlSNAT-overexpressing (SlSNAT-OE) transgenic rice plants showed higher levels of melatonin synthesis than wild-type controls. The SlSNAT-OE rice plants exhibited delayed leaf senescence and tolerance against treatment with the reactive oxygen species (ROS)-inducing herbicide butafenacil by decreasing hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels, suggesting that melatonin alleviates ROS production in vivo.
{"title":"Functional Characterization of the Ciliate <i>Stylonychia lemnae</i> Serotonin <i>N</i>-Acetyltransferase, a Pivotal Enzyme in Melatonin Biosynthesis and Its Overexpression Leads to Peroxidizing Herbicide Tolerance in Rice.","authors":"Kyungjin Lee, Kyoungwhan Back","doi":"10.3390/antiox13101177","DOIUrl":"10.3390/antiox13101177","url":null,"abstract":"<p><p>Serotonin <i>N</i>-acetyltransferase (SNAT) is a pivotal enzyme for melatonin biosynthesis in all living organisms. It catalyzes the conversion of serotonin to <i>N</i>-acetylserotonin (NAS) or 5-methoxytrypytamine (5-MT) to melatonin. In contrast to animal- and plant-specific <i>SNAT</i> genes, a novel clade of archaeal <i>SNAT</i> genes has recently been reported. In this study, we identified homologues of archaeal <i>SNAT</i> genes in ciliates and dinoflagellates, but no animal- or plant-specific <i>SNAT</i> homologues. Archaeal <i>SNAT</i> homologue from the ciliate <i>Stylonychia lemnae</i> was annotated as a putative <i>N</i>-acetyltransferase. To determine whether the putative <i>S. lemnae SNAT</i> (<i>SlSNAT</i>) exhibits SNAT enzyme activity, we chemically synthesized and expressed the full-length <i>SlSNAT</i> coding sequence (CDS) in <i>Escherichia coli</i>, from which the recombinant SlSNAT protein was purified by Ni<sup>2+</sup> affinity column chromatography. The recombinant SlSNAT exhibited SNAT enzyme activity toward serotonin (<i>K</i><sub>m</sub> = 776 µM) and 5-MT (<i>K</i><sub>m</sub> = 246 µM) as substrates. Furthermore, <i>SlSNAT</i>-overexpressing (SlSNAT-OE) transgenic rice plants showed higher levels of melatonin synthesis than wild-type controls. The SlSNAT-OE rice plants exhibited delayed leaf senescence and tolerance against treatment with the reactive oxygen species (ROS)-inducing herbicide butafenacil by decreasing hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and malondialdehyde (MDA) levels, suggesting that melatonin alleviates ROS production in vivo.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":null,"pages":null},"PeriodicalIF":6.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11505474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Heike Brendel, Jennifer Mittag, Anja Hofmann, Helene Hempel, Sindy Giebe, Patrick Diaba-Nuhoho, Steffen Wolk, Christian Reeps, Henning Morawietz, Coy Brunssen
Aim: The primary endothelial NADPH oxidase isoform 4 (NOX4) is notably induced during hypoxia, with emerging evidence suggesting its vasoprotective role through H2O2 production. Therefore, we aimed to elucidate NOX4's significance in endothelial function under hypoxia. Methods: Human vessels, in addition to murine vessels from Nox4-/- mice, were explored. On a functional level, Mulvany myograph experiments were performed. To obtain mechanistical insights, human endothelial cells were cultured under hypoxia with inhibitors of hypoxia-inducible factors. Additionally, endothelial cells were cultured under combined hypoxia and laminar shear stress conditions. Results: In human occluded vessels, NOX4 expression strongly correlated with prostaglandin I2 synthase (PTGIS). Hypoxia significantly elevated NOX4 and PTGIS expression and activity in human endothelial cells. Inhibition of prolyl hydroxylase domain (PHD) enzymes, which stabilize hypoxia-inducible factors (HIFs), increased NOX4 and PTGIS expression even under normoxic conditions. NOX4 mRNA expression was reduced by HIF1a inhibition, while PTGIS mRNA expression was only affected by the inhibition of HIF2a under hypoxia. Endothelial function assessments revealed hypoxia-induced endothelial dysfunction in mesenteric arteries from wild-type mice. Mesenteric arteries from Nox4-/- mice exhibited an altered endothelial function under hypoxia, most prominent in the presence of cyclooxygenase inhibitor diclofenac to exclude the impact of prostacyclin. Restored protective laminar shear stress, as it might occur after thrombolysis, angioplasty, or stenting, attenuated the hypoxic response in endothelial cells, reducing HIF1a expression and its target NOX4 while enhancing eNOS expression. Conclusions: Hypoxia strongly induces NOX4 and PTGIS, with a close correlation between both factors in occluded, hypoxic human vessels. This relationship ensured endothelium-dependent vasodilation under hypoxic conditions. Protective laminar blood flow restores eNOS expression and mitigates the hypoxic response on NOX4 and PTGIS.
{"title":"NADPH Oxidase 4: Crucial for Endothelial Function under Hypoxia-Complementing Prostacyclin.","authors":"Heike Brendel, Jennifer Mittag, Anja Hofmann, Helene Hempel, Sindy Giebe, Patrick Diaba-Nuhoho, Steffen Wolk, Christian Reeps, Henning Morawietz, Coy Brunssen","doi":"10.3390/antiox13101178","DOIUrl":"10.3390/antiox13101178","url":null,"abstract":"<p><p><i>Aim</i>: The primary endothelial NADPH oxidase isoform 4 (NOX4) is notably induced during hypoxia, with emerging evidence suggesting its vasoprotective role through H<sub>2</sub>O<sub>2</sub> production. Therefore, we aimed to elucidate NOX4's significance in endothelial function under hypoxia. <i>Methods</i>: Human vessels, in addition to murine vessels from <i>Nox4<sup>-/-</sup></i> mice, were explored. On a functional level, Mulvany myograph experiments were performed. To obtain mechanistical insights, human endothelial cells were cultured under hypoxia with inhibitors of hypoxia-inducible factors. Additionally, endothelial cells were cultured under combined hypoxia and laminar shear stress conditions. <i>Results</i>: In human occluded vessels, NOX4 expression strongly correlated with prostaglandin I2 synthase (<i>PTGIS</i>). Hypoxia significantly elevated NOX4 and PTGIS expression and activity in human endothelial cells. Inhibition of prolyl hydroxylase domain (PHD) enzymes, which stabilize hypoxia-inducible factors (HIFs), increased NOX4 and PTGIS expression even under normoxic conditions. <i>NOX4</i> mRNA expression was reduced by HIF1a inhibition, while <i>PTGIS</i> mRNA expression was only affected by the inhibition of HIF2a under hypoxia. Endothelial function assessments revealed hypoxia-induced endothelial dysfunction in mesenteric arteries from wild-type mice. Mesenteric arteries from <i>Nox4<sup>-/-</sup></i> mice exhibited an altered endothelial function under hypoxia, most prominent in the presence of cyclooxygenase inhibitor diclofenac to exclude the impact of prostacyclin. Restored protective laminar shear stress, as it might occur after thrombolysis, angioplasty, or stenting, attenuated the hypoxic response in endothelial cells, reducing HIF1a expression and its target <i>NOX4</i> while enhancing <i>eNOS</i> expression. <i>Conclusions</i>: Hypoxia strongly induces NOX4 and PTGIS, with a close correlation between both factors in occluded, hypoxic human vessels. This relationship ensured endothelium-dependent vasodilation under hypoxic conditions. Protective laminar blood flow restores eNOS expression and mitigates the hypoxic response on NOX4 and PTGIS.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":null,"pages":null},"PeriodicalIF":6.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11504732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Augustin C Barolet, Brice Magne, Daniel Barolet, Lucie Germain
NO is a crucial signaling molecule involved in skin health, the immune response, and the protection against environmental stressors. This study explores how different wavelengths of light, namely blue (455 nm), red (660 nm), and near infrared (NIR, 850 nm), affect nitric oxide (NO) production in skin cells. Primary keratinocytes and fibroblasts from three donors were exposed to these wavelengths, and NO production was quantified using a DAF-FM fluorescent probe. The results demonstrated that all three wavelengths stimulated NO release, with blue light showing the most pronounced effect. Specifically, blue light induced a 1.7-fold increase in NO in keratinocytes compared to red and NIR light and a 2.3-fold increase in fibroblasts compared to red light. Notably, fibroblasts exposed to NIR light produced 1.5 times more NO than those exposed to red light, while keratinocytes consistently responded more robustly across all wavelengths. In conclusion, blue light significantly boosts NO production in both keratinocytes and fibroblasts, making it the most effective wavelength. Red and NIR light, while less potent, also promote NO production and could serve as complementary therapeutic options, particularly for minimizing potential photoaging effects.
一氧化氮是参与皮肤健康、免疫反应和抵御环境压力的重要信号分子。本研究探讨了不同波长的光,即蓝光(455 nm)、红光(660 nm)和近红外线(NIR,850 nm)如何影响皮肤细胞中一氧化氮(NO)的产生。将来自三位供体的原代角质细胞和成纤维细胞暴露在这些波长下,并使用 DAF-FM 荧光探针对一氧化氮的产生进行量化。结果表明,三种波长的光都能刺激一氧化氮的释放,其中蓝光的效果最明显。具体来说,与红光和近红外光相比,蓝光诱导角质细胞中的 NO 增加了 1.7 倍,与红光相比,成纤维细胞中的 NO 增加了 2.3 倍。值得注意的是,暴露在近红外光下的成纤维细胞产生的 NO 是暴露在红光下的成纤维细胞的 1.5 倍,而角质细胞在所有波长下的反应都更为强烈。总之,蓝光能显著促进角质细胞和成纤维细胞产生 NO,是最有效的波长。红光和近红外光虽然效力较弱,但也能促进氮氧化物的产生,可以作为补充治疗方案,尤其是在最大限度地减少潜在的光老化效应方面。
{"title":"Differential Nitric Oxide Responses in Primary Cultured Keratinocytes and Fibroblasts to Visible and Near-Infrared Light.","authors":"Augustin C Barolet, Brice Magne, Daniel Barolet, Lucie Germain","doi":"10.3390/antiox13101176","DOIUrl":"10.3390/antiox13101176","url":null,"abstract":"<p><p>NO is a crucial signaling molecule involved in skin health, the immune response, and the protection against environmental stressors. This study explores how different wavelengths of light, namely blue (455 nm), red (660 nm), and near infrared (NIR, 850 nm), affect nitric oxide (NO) production in skin cells. Primary keratinocytes and fibroblasts from three donors were exposed to these wavelengths, and NO production was quantified using a DAF-FM fluorescent probe. The results demonstrated that all three wavelengths stimulated NO release, with blue light showing the most pronounced effect. Specifically, blue light induced a 1.7-fold increase in NO in keratinocytes compared to red and NIR light and a 2.3-fold increase in fibroblasts compared to red light. Notably, fibroblasts exposed to NIR light produced 1.5 times more NO than those exposed to red light, while keratinocytes consistently responded more robustly across all wavelengths. In conclusion, blue light significantly boosts NO production in both keratinocytes and fibroblasts, making it the most effective wavelength. Red and NIR light, while less potent, also promote NO production and could serve as complementary therapeutic options, particularly for minimizing potential photoaging effects.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":null,"pages":null},"PeriodicalIF":6.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11504005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bufotalin (BT), a major active constituent of Chansu, has been found to possess multiple pharmacological activities. Although previous studies have shown that BT could inhibit the growth of glioblastoma (GBM), the safety of BT in vivo and the potential mechanism are still unclear. We conducted a systematic assessment to investigate the impact of BT on GBM cell viability, migration, invasion, and colony formation. Furthermore, in vivo results were obtained to evaluate the effect of BT on tumor growth. The preliminary findings of our study demonstrate the effective inhibition of GBM cell growth and subcutaneous tumor development in mice by BT, with tolerable levels of tolerance observed. Mechanistically, BT treatment induced mitochondrial dysfunction, bursts of reactive oxygen species (ROS), and subsequent cell apoptosis. More importantly, proteomic-based differentially expressed proteins analysis revealed a significant downregulation of integrin β4 (ITGB4) following BT treatment. Furthermore, our evidence suggested that the ITGB4/focal adhesion kinase (FAK)/extracellular signal-related kinase (ERK) pathway involved BT-induced apoptosis. Overall, our study demonstrates the anti-GBM effects of BT and elucidates the underlying mechanism, highlighting BT as a potential therapeutic option for GBM.
{"title":"Bufotalin Induces Oxidative Stress-Mediated Apoptosis by Blocking the ITGB4/FAK/ERK Pathway in Glioblastoma.","authors":"Junchao Tan, Guoqiang Lin, Rui Zhang, Yuting Wen, Chunying Luo, Ran Wang, Feiyun Wang, Shoujiao Peng, Jiange Zhang","doi":"10.3390/antiox13101179","DOIUrl":"10.3390/antiox13101179","url":null,"abstract":"<p><p>Bufotalin (BT), a major active constituent of Chansu, has been found to possess multiple pharmacological activities. Although previous studies have shown that BT could inhibit the growth of glioblastoma (GBM), the safety of BT in vivo and the potential mechanism are still unclear. We conducted a systematic assessment to investigate the impact of BT on GBM cell viability, migration, invasion, and colony formation. Furthermore, in vivo results were obtained to evaluate the effect of BT on tumor growth. The preliminary findings of our study demonstrate the effective inhibition of GBM cell growth and subcutaneous tumor development in mice by BT, with tolerable levels of tolerance observed. Mechanistically, BT treatment induced mitochondrial dysfunction, bursts of reactive oxygen species (ROS), and subsequent cell apoptosis. More importantly, proteomic-based differentially expressed proteins analysis revealed a significant downregulation of integrin β4 (ITGB4) following BT treatment. Furthermore, our evidence suggested that the ITGB4/focal adhesion kinase (FAK)/extracellular signal-related kinase (ERK) pathway involved BT-induced apoptosis. Overall, our study demonstrates the anti-GBM effects of BT and elucidates the underlying mechanism, highlighting BT as a potential therapeutic option for GBM.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":null,"pages":null},"PeriodicalIF":6.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11505062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oxidative stress, characterized by an imbalance between the production of reactive oxygen species (ROS) and the body's antioxidant defenses, significantly affects cellular function and viability. It plays a pivotal role in modulating membrane potentials, particularly action potentials (APs), essential for properly functioning excitable cells such as neurons, smooth muscles, pancreatic beta cells, and myocytes. The interaction between oxidative stress and AP dynamics is crucial for understanding the pathophysiology of various conditions, including neurodegenerative diseases, cardiac arrhythmias, and ischemia-reperfusion injuries. This review explores how oxidative stress influences APs, focusing on alterations in ion channel biophysics, gap junction, calcium dynamics, mitochondria, and Interstitial Cells of Cajal functions. By integrating current research, we aim to elucidate how oxidative stress contributes to disease progression and discuss potential therapeutic interventions targeting this interaction.
{"title":"Modulatory Impact of Oxidative Stress on Action Potentials in Pathophysiological States: A Comprehensive Review.","authors":"Chitaranjan Mahapatra, Ravindra Thakkar, Ravinder Kumar","doi":"10.3390/antiox13101172","DOIUrl":"10.3390/antiox13101172","url":null,"abstract":"<p><p>Oxidative stress, characterized by an imbalance between the production of reactive oxygen species (ROS) and the body's antioxidant defenses, significantly affects cellular function and viability. It plays a pivotal role in modulating membrane potentials, particularly action potentials (APs), essential for properly functioning excitable cells such as neurons, smooth muscles, pancreatic beta cells, and myocytes. The interaction between oxidative stress and AP dynamics is crucial for understanding the pathophysiology of various conditions, including neurodegenerative diseases, cardiac arrhythmias, and ischemia-reperfusion injuries. This review explores how oxidative stress influences APs, focusing on alterations in ion channel biophysics, gap junction, calcium dynamics, mitochondria, and Interstitial Cells of Cajal functions. By integrating current research, we aim to elucidate how oxidative stress contributes to disease progression and discuss potential therapeutic interventions targeting this interaction.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":null,"pages":null},"PeriodicalIF":6.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11504047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}