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Early Weaning Inhibits Intestinal Stem Cell Expansion to Disrupt the Intestinal Integrity of Duroc Piglets via Regulating the Keap1/Nrf2 Signaling. 早期断奶通过调控 Keap1/Nrf2 信号抑制杜洛克仔猪肠干细胞扩增以破坏肠道完整性
IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-30 DOI: 10.3390/antiox13101188
Ying-Chao Qin, Cheng-Long Jin, Ting-Cai Hu, Jia-Yi Zhou, Xiao-Fan Wang, Xiu-Qi Wang, Xiang-Feng Kong, Hui-Chao Yan

There are different stress resistance among different breeds of pigs. Changes in intestinal stem cells (ISCs) are still unclear among various breeds of piglets after early weaning. In the current study, Taoyuan Black and Duroc piglets were slaughtered at 21 days of age (early weaning day) and 24 days of age (3 days after early weaning) for 10 piglets in each group. The results showed that the rate of ISC-driven epithelial renewal in local Taoyuan Black pigs hardly changed after weaning for 3 days. However, weaning stress significantly reduced the weight of the duodenum and jejunum in Duroc piglets. Meanwhile, the jejunal villus height, tight junction-related proteins (ZO-1, Occludin, and Claudin1), as well as the trans-epithelial electrical resistance (TEER) values, were down-regulated after weaning for 3 days in Duroc piglets. Moreover, compared with Unweaned Duroc piglets, the numbers of Olfm4+ ISC cells, PCNA+ mitotic cells, SOX9+ secretory progenitor cells, and Villin+ absorptive cells in the jejunum were reduced significantly 3 days after weaning. And ex vivo jejunal crypt-derived organoids exhibited growth disadvantages in weaned Duroc piglets. Notably, the Keap1/Nrf2 signaling activities and the expression of HO-1 were significantly depressed in weaned Duroc piglets compared to Unweaned Duroc piglets. Thus, we can conclude that ISCs of Duroc piglets were more sensitive to weaning stress injury than Taoyuan Black piglets, and Keap1/Nrf2 signaling is involved in this process.

不同品种的猪具有不同的抗应激能力。不同品种的仔猪在早期断奶后肠干细胞(ISCs)的变化仍不清楚。在本研究中,桃源黑猪和杜洛克仔猪分别在 21 日龄(早期断奶日)和 24 日龄(早期断奶后 3 天)屠宰,每组 10 头仔猪。结果表明,当地桃源黑猪在断奶 3 天后,ISC 驱动的上皮更新率几乎没有变化。然而,断奶应激明显降低了杜洛克仔猪十二指肠和空肠的重量。同时,断奶3天后,杜洛克仔猪的空肠绒毛高度、紧密连接相关蛋白(ZO-1、Occludin和Claudin1)以及跨上皮电阻(TEER)值均出现下调。此外,与未断奶杜洛克仔猪相比,断奶 3 天后空肠中 Olfm4+ ISC 细胞、PCNA+ 有丝分裂细胞、SOX9+ 分泌祖细胞和 Villin+ 吸收细胞的数量显著减少。断奶后的杜洛克仔猪体内空肠隐窝衍生的器官组织表现出生长劣势。值得注意的是,与未断奶杜洛克仔猪相比,断奶杜洛克仔猪的Keap1/Nrf2信号活性和HO-1的表达均显著降低。因此,我们可以得出结论:与桃源黑猪相比,杜洛克仔猪的ISC对断奶应激损伤更敏感,而Keap1/Nrf2信号转导参与了这一过程。
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引用次数: 0
Reactive Oxygen Species in Cystic Kidney Disease. 囊性肾病中的活性氧。
IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-30 DOI: 10.3390/antiox13101186
Sanat Subhash, Sonya Vijayvargiya, Aetan Parmar, Jazlyn Sandhu, Jabrina Simmons, Rupesh Raina

Polycystic kidney disease (PKD) is a rare but significant renal condition with major implications for global acute and chronic patient care. Oxidative stress and reactive oxygen species (ROS) can significantly alter its pathophysiology, clinical outcomes, and treatment, contributing to negative outcomes, including hypertension, chronic kidney disease, and kidney failure. Inflammation from ROS and existing cysts propagate the generation and accumulation of ROS, exacerbating kidney injury, pro-fibrotic signaling cascades, and interstitial fibrosis. Early identification and prevention of oxidative stress and ROS can contribute to reduced cystic kidney disease progression and improved longitudinal patient outcomes. Increased research regarding biomarkers, the pathophysiology of oxidative stress, and novel therapeutic interventions alongside the creation of comprehensive guidelines establishing methods of assessment, monitoring, and intervention for oxidative stress in cystic kidney disease patients is imperative to standardize clinical practice and improve patient outcomes. The integration of artificial intelligence (AI), genetic editing, and genome sequencing could further improve the early detection and management of cystic kidney disease and mitigate adverse patient outcomes. In this review, we aim to comprehensively assess the multifactorial role of ROS in cystic kidney disease, analyzing its pathophysiology, clinical outcomes, treatment interventions, clinical trials, animal models, and future directions for patient care.

多囊肾(PKD)是一种罕见但严重的肾病,对全球急性和慢性病患者的护理具有重大影响。氧化应激和活性氧(ROS)可显著改变其病理生理学、临床结果和治疗,导致高血压、慢性肾病和肾衰竭等不良后果。ROS 引起的炎症和现有的囊肿会促进 ROS 的产生和积累,加剧肾脏损伤、促纤维化信号级联和间质纤维化。氧化应激和 ROS 的早期识别和预防有助于减少囊性肾脏疾病的进展和改善患者的纵向预后。为了规范临床实践和改善患者预后,必须加强对生物标志物、氧化应激病理生理学和新型治疗干预措施的研究,同时制定综合指南,确定评估、监测和干预囊性肾脏病患者氧化应激的方法。人工智能(AI)、基因编辑和基因组测序的整合可进一步改善囊性肾脏病的早期检测和管理,减轻患者的不良预后。在这篇综述中,我们旨在全面评估 ROS 在囊性肾脏病中的多因素作用,分析其病理生理学、临床结果、治疗干预、临床试验、动物模型以及患者护理的未来方向。
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引用次数: 0
Chemical Composition and Antioxidant Activity of Six Allium Extracts Using Protein-Based Biomimetic Methods. 利用基于蛋白质的仿生方法研究六种薤白提取物的化学成分和抗氧化活性
IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-29 DOI: 10.3390/antiox13101182
Ioana Andreea Barbu, Vlad Alexandru Toma, Augustin Cătălin Moț, Ana-Maria Vlase, Anca Butiuc-Keul, Marcel Pârvu

Medicinal plants are a valuable reservoir of novel pharmacologically active compounds. ROS and free radicals are primary contributors to oxidative stress, a condition associated with the onset of degenerative diseases such as cancer, coronary heart disease, and vascular disease. In this study, we used different spectrophotometry methods to demonstrate the antioxidant properties of 6 Allium extracts: Allium fistulosum; Allium ursinum; Allium cepa: Arieș red cultivar of A. cepa, and white variety of A. cepa; Allium sativum; and Allium senescens subsp. montanum. HPLC-MS determined the chemical composition of the extracts. Among the tested extracts, the Arieș red cultivar of A. cepa stands out as having the best antioxidant activity, probably due to the high content of polyphenols and alliin (12.67 µg/mL and 3565 ng/mL, respectively). The results obtained in this study show that Allium extracts have antioxidant activity, but also free radical scavenging capabilities. Also, their interactions with cytochrome c and hemoglobin can be the basis of future studies to create treatments for oxidative stress-related diseases.

药用植物是新型药理活性化合物的宝贵宝库。ROS 和自由基是导致氧化应激的主要因素,而氧化应激与癌症、冠心病和血管疾病等退行性疾病的发生有关。在这项研究中,我们使用了不同的分光光度法来证明 6 种薤白提取物的抗氧化特性:这 6 种薤白提取物分别是:瘘管薤白、乌苏薤白、牛肝菌薤白:牛肝菌薤白红色栽培品种和牛肝菌薤白白色品种、荠菜薤白和莴苣薤白亚种。HPLC-MS 测定了提取物的化学成分。在测试的萃取物中,Arieș red 栽培品种牛肝菌的抗氧化活性最好,这可能是由于多酚和蒜氨酸的含量较高(分别为 12.67 µg/mL 和 3565 ng/mL)。本研究的结果表明,薤白提取物不仅具有抗氧化活性,还具有清除自由基的能力。此外,薤白提取物与细胞色素 c 和血红蛋白之间的相互作用也可作为今后研究氧化应激相关疾病治疗方法的基础。
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引用次数: 0
One-Year Impact of Occupational Exposure to Polycyclic Aromatic Hydrocarbons on Sperm Quality. 职业暴露于多环芳烃对精子质量的一年影响
IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-29 DOI: 10.3390/antiox13101181
Mª Victoria Peña-García, Mª José Moyano-Gallego, Sara Gómez-Melero, Rafael Molero-Payán, Fernando Rodríguez-Cantalejo, Javier Caballero-Villarraso

Background: Polycyclic aromatic hydrocarbons (PAHs) have toxic potential, especially as carcinogens, neurotoxins, and endocrine disruptors. The objective of this study is to know the impact of exposure to PAHs on the reproductive health of male workers who operate in solar thermal plants.

Methods: Case-control study. A total of 61 men were included: 32 workers exposed to PAH at a solar thermal plant and 29 unexposed people. Seminal quality was studied both at the cellular level (quantity and quality of sperm) and at the biochemical level (magnitudes of oxidative stress in seminal plasma).

Results: In exposure to PAHs, a significantly higher seminal leukocyte infiltration was observed, as well as lower activity in seminal plasma of superoxide dismutase (SOD) and a reduced glutathione/oxidised glutathione (GSH/GSSG) ratio. The oxidative stress parameters of seminal plasma did not show a relationship with sperm cellularity, neither in those exposed nor in those not exposed to PAH.

Conclusion: One year of exposure to PAH in a solar thermal plant does not have a negative impact on the sperm cellularity of the worker, either quantitatively (sperm count) or qualitatively (motility, vitality, morphology, or cellular DNA fragmentation). However, PAH exposure is associated with lower antioxidant capacity and higher leukocyte infiltration in seminal plasma.

背景:多环芳烃(PAHs)具有潜在毒性,尤其是作为致癌物、神经毒素和内分泌干扰物。本研究的目的是了解接触多环芳烃对光热厂男工生殖健康的影响:方法:病例对照研究。方法:病例对照研究:方法:病例对照研究共纳入 61 名男性:32 名接触多环芳烃的太阳能热电厂工人和 29 名未接触者。从细胞水平(精子的数量和质量)和生化水平(精浆中氧化应激的大小)对精液质量进行了研究:结果:在暴露于多环芳烃的情况下,观察到精液中的白细胞浸润明显增加,精浆中超氧化物歧化酶(SOD)的活性和还原型谷胱甘肽/氧化型谷胱甘肽(GSH/GSSG)的比率降低。无论是暴露于多环芳烃还是未暴露于多环芳烃的人群,精浆中的氧化应激参数均未显示出与精子细胞性的关系:结论:在太阳能热电厂工作一年并接触多环芳烃不会对工人的精子细胞数量(精子数量)或质量(活力、生命力、形态或细胞 DNA 片段)产生负面影响。然而,接触多环芳烃会导致抗氧化能力降低和精浆中白细胞浸润增加。
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引用次数: 0
Different Oxidative Stress and Inflammation Patterns of Diseased Left Anterior Descending Coronary Artery versus Internal Thoracic Artery. 病变左前降支冠状动脉与胸内动脉的氧化应激和炎症模式不同
IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-28 DOI: 10.3390/antiox13101180
Andrea Salica, Vittoria Cammisotto, Raffaele Scaffa, Giulio Folino, Ruggero De Paulis, Roberto Carnevale, Umberto Benedetto, Wael Saade, Antonino Marullo, Sebastiano Sciarretta, Gianmarco Sarto, Silvia Palmerio, Valentina Valenti, Mariangela Peruzzi, Fabio Miraldi, Francesco Giosuè Irace, Giacomo Frati

Background: Oxidative stress and inflammation are typically implied in atherosclerosis pathogenesis and progression, especially in coronary artery disease (CAD). Our objective was to investigate the oxidative stress and inflammation burden directly associated with atherosclerotic plaque in patients with stable coronary disease undergoing coronary artery bypass graft (CABG) surgery. Specifically, markers of oxidative stress and inflammation were compared in blood samples obtained from the atherosclerotic left anterior descending artery (LAD) and blood samples obtained from the healthy left internal thoracic artery (LITA), used as a bypass graft, within the same patient.

Methods: Twenty patients scheduled for off-pump CABG were enrolled. Blood samples were collected from the LITA below anastomosis and the LAD below the stenosis. Samples were analysed for oxidative stress (sNOXdp, H2O2, NO) and inflammation markers (TNFα, IL-6, IL-1β, IL-10).

Results: The analysis showed a significant increase in oxidative stress burden in the LAD as compared to LITA, as indicated by higher sNOX2-dp and H2O2 levels and lower NO levels (p < 0.01). Also, pro-inflammatory cytokines were increased in the LAD as compared to the LITA, as indicated by higher TNFα and IL-6 amounts (p < 0.01). On the other hand, no significant differences could be seen regarding IL-1β and IL-10 levels between the two groups.

Conclusions: The oxidative stress and inflammatory burden are specifically enhanced in the LAD artery of stable coronary patients compared to systemic blood from the LITA of stable coronary patients.

背景:氧化应激和炎症通常与动脉粥样硬化的发病和进展有关,尤其是在冠状动脉疾病(CAD)中。我们的目的是研究接受冠状动脉旁路移植(CABG)手术的稳定型冠心病患者体内与动脉粥样硬化斑块直接相关的氧化应激和炎症负担。具体来说,比较了同一患者从动脉粥样硬化的左前降支动脉(LAD)采集的血液样本和从健康的左胸内动脉(LITA)(用作旁路移植)采集的血液样本中的氧化应激和炎症指标:方法:20 名计划接受非泵 CABG 手术的患者入组。从吻合口下方的 LITA 和狭窄处下方的 LAD 采集血液样本。对样本进行氧化应激(sNOXdp、H2O2、NO)和炎症指标(TNFα、IL-6、IL-1β、IL-10)分析:分析表明,与LITA相比,LAD的氧化应激负荷明显增加,表现为sNOX2-dp和H2O2水平升高,NO水平降低(P < 0.01)。此外,与 LITA 相比,LAD 中的促炎细胞因子增加,表现为 TNFα 和 IL-6 水平升高(p < 0.01)。另一方面,两组间的IL-1β和IL-10水平无明显差异:结论:与来自稳定期冠心病患者 LITA 的全身血液相比,稳定期冠心病患者 LAD 动脉中的氧化应激和炎症负荷明显增加。
{"title":"Different Oxidative Stress and Inflammation Patterns of Diseased Left Anterior Descending Coronary Artery versus Internal Thoracic Artery.","authors":"Andrea Salica, Vittoria Cammisotto, Raffaele Scaffa, Giulio Folino, Ruggero De Paulis, Roberto Carnevale, Umberto Benedetto, Wael Saade, Antonino Marullo, Sebastiano Sciarretta, Gianmarco Sarto, Silvia Palmerio, Valentina Valenti, Mariangela Peruzzi, Fabio Miraldi, Francesco Giosuè Irace, Giacomo Frati","doi":"10.3390/antiox13101180","DOIUrl":"10.3390/antiox13101180","url":null,"abstract":"<p><strong>Background: </strong>Oxidative stress and inflammation are typically implied in atherosclerosis pathogenesis and progression, especially in coronary artery disease (CAD). Our objective was to investigate the oxidative stress and inflammation burden directly associated with atherosclerotic plaque in patients with stable coronary disease undergoing coronary artery bypass graft (CABG) surgery. Specifically, markers of oxidative stress and inflammation were compared in blood samples obtained from the atherosclerotic left anterior descending artery (LAD) and blood samples obtained from the healthy left internal thoracic artery (LITA), used as a bypass graft, within the same patient.</p><p><strong>Methods: </strong>Twenty patients scheduled for off-pump CABG were enrolled. Blood samples were collected from the LITA below anastomosis and the LAD below the stenosis. Samples were analysed for oxidative stress (sNOXdp, H<sub>2</sub>O<sub>2</sub>, NO) and inflammation markers (TNFα, IL-6, IL-1β, IL-10).</p><p><strong>Results: </strong>The analysis showed a significant increase in oxidative stress burden in the LAD as compared to LITA, as indicated by higher sNOX2-dp and H<sub>2</sub>O<sub>2</sub> levels and lower NO levels (<i>p</i> < 0.01). Also, pro-inflammatory cytokines were increased in the LAD as compared to the LITA, as indicated by higher TNFα and IL-6 amounts (<i>p</i> < 0.01). On the other hand, no significant differences could be seen regarding IL-1β and IL-10 levels between the two groups.</p><p><strong>Conclusions: </strong>The oxidative stress and inflammatory burden are specifically enhanced in the LAD artery of stable coronary patients compared to systemic blood from the LITA of stable coronary patients.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"13 10","pages":""},"PeriodicalIF":6.0,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11505158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Functional Characterization of the Ciliate Stylonychia lemnae Serotonin N-Acetyltransferase, a Pivotal Enzyme in Melatonin Biosynthesis and Its Overexpression Leads to Peroxidizing Herbicide Tolerance in Rice. 纤毛虫 Stylonychia lemnae Serotonin N-Acetyltransferase 的功能特征,它是褪黑激素生物合成过程中的关键酶,其过度表达导致水稻耐过氧化性除草剂。
IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-27 DOI: 10.3390/antiox13101177
Kyungjin Lee, Kyoungwhan Back

Serotonin N-acetyltransferase (SNAT) is a pivotal enzyme for melatonin biosynthesis in all living organisms. It catalyzes the conversion of serotonin to N-acetylserotonin (NAS) or 5-methoxytrypytamine (5-MT) to melatonin. In contrast to animal- and plant-specific SNAT genes, a novel clade of archaeal SNAT genes has recently been reported. In this study, we identified homologues of archaeal SNAT genes in ciliates and dinoflagellates, but no animal- or plant-specific SNAT homologues. Archaeal SNAT homologue from the ciliate Stylonychia lemnae was annotated as a putative N-acetyltransferase. To determine whether the putative S. lemnae SNAT (SlSNAT) exhibits SNAT enzyme activity, we chemically synthesized and expressed the full-length SlSNAT coding sequence (CDS) in Escherichia coli, from which the recombinant SlSNAT protein was purified by Ni2+ affinity column chromatography. The recombinant SlSNAT exhibited SNAT enzyme activity toward serotonin (Km = 776 µM) and 5-MT (Km = 246 µM) as substrates. Furthermore, SlSNAT-overexpressing (SlSNAT-OE) transgenic rice plants showed higher levels of melatonin synthesis than wild-type controls. The SlSNAT-OE rice plants exhibited delayed leaf senescence and tolerance against treatment with the reactive oxygen species (ROS)-inducing herbicide butafenacil by decreasing hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels, suggesting that melatonin alleviates ROS production in vivo.

羟色胺 N-乙酰转移酶(SNAT)是所有生物体内褪黑素生物合成的关键酶。它催化血清素向 N-乙酰丝氨酸(NAS)或 5-甲氧基色胺(5-MT)向褪黑激素的转化。与动物和植物特异性 SNAT 基因不同,最近报道了一个新的古菌 SNAT 基因群。在这项研究中,我们在纤毛虫和甲藻中发现了古生SNAT基因的同源物,但没有发现动物或植物特有的SNAT同源物。来自纤毛虫Stylonychia lemnae的古SNAT同源基因被注释为一种推定的N-乙酰转移酶。为了确定S. lemnae SNAT(SlSNAT)是否具有SNAT酶活性,我们在大肠杆菌中化学合成并表达了全长的SlSNAT编码序列(CDS),并通过Ni2+亲和柱色谱法纯化了重组SlSNAT蛋白。重组的 SlSNAT 对血清素(Km = 776 µM)和 5-甲基硫醇(Km = 246 µM)具有 SNAT 酶活性。此外,与野生型对照相比,SlSNAT-OE 转基因水稻植株的褪黑激素合成水平更高。通过降低过氧化氢(H2O2)和丙二醛(MDA)水平,SlSNAT-OE 水稻植株表现出延迟叶片衰老和对活性氧(ROS)诱导除草剂丁氟螨酯的耐受性,这表明褪黑激素能减轻体内 ROS 的产生。
{"title":"Functional Characterization of the Ciliate <i>Stylonychia lemnae</i> Serotonin <i>N</i>-Acetyltransferase, a Pivotal Enzyme in Melatonin Biosynthesis and Its Overexpression Leads to Peroxidizing Herbicide Tolerance in Rice.","authors":"Kyungjin Lee, Kyoungwhan Back","doi":"10.3390/antiox13101177","DOIUrl":"10.3390/antiox13101177","url":null,"abstract":"<p><p>Serotonin <i>N</i>-acetyltransferase (SNAT) is a pivotal enzyme for melatonin biosynthesis in all living organisms. It catalyzes the conversion of serotonin to <i>N</i>-acetylserotonin (NAS) or 5-methoxytrypytamine (5-MT) to melatonin. In contrast to animal- and plant-specific <i>SNAT</i> genes, a novel clade of archaeal <i>SNAT</i> genes has recently been reported. In this study, we identified homologues of archaeal <i>SNAT</i> genes in ciliates and dinoflagellates, but no animal- or plant-specific <i>SNAT</i> homologues. Archaeal <i>SNAT</i> homologue from the ciliate <i>Stylonychia lemnae</i> was annotated as a putative <i>N</i>-acetyltransferase. To determine whether the putative <i>S. lemnae SNAT</i> (<i>SlSNAT</i>) exhibits SNAT enzyme activity, we chemically synthesized and expressed the full-length <i>SlSNAT</i> coding sequence (CDS) in <i>Escherichia coli</i>, from which the recombinant SlSNAT protein was purified by Ni<sup>2+</sup> affinity column chromatography. The recombinant SlSNAT exhibited SNAT enzyme activity toward serotonin (<i>K</i><sub>m</sub> = 776 µM) and 5-MT (<i>K</i><sub>m</sub> = 246 µM) as substrates. Furthermore, <i>SlSNAT</i>-overexpressing (SlSNAT-OE) transgenic rice plants showed higher levels of melatonin synthesis than wild-type controls. The SlSNAT-OE rice plants exhibited delayed leaf senescence and tolerance against treatment with the reactive oxygen species (ROS)-inducing herbicide butafenacil by decreasing hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and malondialdehyde (MDA) levels, suggesting that melatonin alleviates ROS production in vivo.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"13 10","pages":""},"PeriodicalIF":6.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11505474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
NADPH Oxidase 4: Crucial for Endothelial Function under Hypoxia-Complementing Prostacyclin. NADPH 氧化酶 4:缺氧条件下内皮功能的关键--补充前列环素。
IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-27 DOI: 10.3390/antiox13101178
Heike Brendel, Jennifer Mittag, Anja Hofmann, Helene Hempel, Sindy Giebe, Patrick Diaba-Nuhoho, Steffen Wolk, Christian Reeps, Henning Morawietz, Coy Brunssen

Aim: The primary endothelial NADPH oxidase isoform 4 (NOX4) is notably induced during hypoxia, with emerging evidence suggesting its vasoprotective role through H2O2 production. Therefore, we aimed to elucidate NOX4's significance in endothelial function under hypoxia. Methods: Human vessels, in addition to murine vessels from Nox4-/- mice, were explored. On a functional level, Mulvany myograph experiments were performed. To obtain mechanistical insights, human endothelial cells were cultured under hypoxia with inhibitors of hypoxia-inducible factors. Additionally, endothelial cells were cultured under combined hypoxia and laminar shear stress conditions. Results: In human occluded vessels, NOX4 expression strongly correlated with prostaglandin I2 synthase (PTGIS). Hypoxia significantly elevated NOX4 and PTGIS expression and activity in human endothelial cells. Inhibition of prolyl hydroxylase domain (PHD) enzymes, which stabilize hypoxia-inducible factors (HIFs), increased NOX4 and PTGIS expression even under normoxic conditions. NOX4 mRNA expression was reduced by HIF1a inhibition, while PTGIS mRNA expression was only affected by the inhibition of HIF2a under hypoxia. Endothelial function assessments revealed hypoxia-induced endothelial dysfunction in mesenteric arteries from wild-type mice. Mesenteric arteries from Nox4-/- mice exhibited an altered endothelial function under hypoxia, most prominent in the presence of cyclooxygenase inhibitor diclofenac to exclude the impact of prostacyclin. Restored protective laminar shear stress, as it might occur after thrombolysis, angioplasty, or stenting, attenuated the hypoxic response in endothelial cells, reducing HIF1a expression and its target NOX4 while enhancing eNOS expression. Conclusions: Hypoxia strongly induces NOX4 and PTGIS, with a close correlation between both factors in occluded, hypoxic human vessels. This relationship ensured endothelium-dependent vasodilation under hypoxic conditions. Protective laminar blood flow restores eNOS expression and mitigates the hypoxic response on NOX4 and PTGIS.

目的:缺氧时会明显诱导初级内皮 NADPH 氧化酶同工酶 4(NOX4),新的证据表明它通过产生 H2O2 起到保护血管的作用。因此,我们旨在阐明 NOX4 在缺氧条件下对内皮功能的重要作用。方法:除了研究 Nox4-/- 小鼠的小鼠血管外,我们还研究了人类血管。在功能层面,进行了 Mulvany 肌电图实验。为了深入了解机理,使用低氧诱导因子抑制剂在低氧条件下培养人类内皮细胞。此外,还在缺氧和层流剪切应力条件下培养内皮细胞。结果在人体闭塞血管中,NOX4的表达与前列腺素I2合成酶(PTGIS)密切相关。缺氧会明显提高人内皮细胞中 NOX4 和 PTGIS 的表达和活性。脯氨酰羟化酶结构域(PHD)酶能稳定缺氧诱导因子(HIF),即使在正常缺氧条件下,抑制该酶也会增加 NOX4 和 PTGIS 的表达。抑制 HIF1a 会降低 NOX4 mRNA 的表达,而 PTGIS mRNA 的表达只受缺氧条件下抑制 HIF2a 的影响。内皮功能评估显示,缺氧诱导野生型小鼠肠系膜动脉内皮功能障碍。Nox4-/-小鼠的肠系膜动脉在缺氧条件下表现出内皮功能的改变,在环氧化酶抑制剂双氯芬酸的作用下最为明显,从而排除了前列环素的影响。在溶栓、血管成形术或支架植入术后恢复的保护性层流剪切应力减轻了内皮细胞的缺氧反应,降低了 HIF1a 的表达及其靶标 NOX4 的表达,同时增强了 eNOS 的表达。结论缺氧会强烈诱导 NOX4 和 PTGIS,在闭塞、缺氧的人体血管中,这两个因素之间存在密切的相关性。这种关系确保了缺氧条件下内皮依赖性血管扩张。保护性层流可恢复 eNOS 的表达,减轻缺氧对 NOX4 和 PTGIS 的影响。
{"title":"NADPH Oxidase 4: Crucial for Endothelial Function under Hypoxia-Complementing Prostacyclin.","authors":"Heike Brendel, Jennifer Mittag, Anja Hofmann, Helene Hempel, Sindy Giebe, Patrick Diaba-Nuhoho, Steffen Wolk, Christian Reeps, Henning Morawietz, Coy Brunssen","doi":"10.3390/antiox13101178","DOIUrl":"10.3390/antiox13101178","url":null,"abstract":"<p><p><i>Aim</i>: The primary endothelial NADPH oxidase isoform 4 (NOX4) is notably induced during hypoxia, with emerging evidence suggesting its vasoprotective role through H<sub>2</sub>O<sub>2</sub> production. Therefore, we aimed to elucidate NOX4's significance in endothelial function under hypoxia. <i>Methods</i>: Human vessels, in addition to murine vessels from <i>Nox4<sup>-/-</sup></i> mice, were explored. On a functional level, Mulvany myograph experiments were performed. To obtain mechanistical insights, human endothelial cells were cultured under hypoxia with inhibitors of hypoxia-inducible factors. Additionally, endothelial cells were cultured under combined hypoxia and laminar shear stress conditions. <i>Results</i>: In human occluded vessels, NOX4 expression strongly correlated with prostaglandin I2 synthase (<i>PTGIS</i>). Hypoxia significantly elevated NOX4 and PTGIS expression and activity in human endothelial cells. Inhibition of prolyl hydroxylase domain (PHD) enzymes, which stabilize hypoxia-inducible factors (HIFs), increased NOX4 and PTGIS expression even under normoxic conditions. <i>NOX4</i> mRNA expression was reduced by HIF1a inhibition, while <i>PTGIS</i> mRNA expression was only affected by the inhibition of HIF2a under hypoxia. Endothelial function assessments revealed hypoxia-induced endothelial dysfunction in mesenteric arteries from wild-type mice. Mesenteric arteries from <i>Nox4<sup>-/-</sup></i> mice exhibited an altered endothelial function under hypoxia, most prominent in the presence of cyclooxygenase inhibitor diclofenac to exclude the impact of prostacyclin. Restored protective laminar shear stress, as it might occur after thrombolysis, angioplasty, or stenting, attenuated the hypoxic response in endothelial cells, reducing HIF1a expression and its target <i>NOX4</i> while enhancing <i>eNOS</i> expression. <i>Conclusions</i>: Hypoxia strongly induces NOX4 and PTGIS, with a close correlation between both factors in occluded, hypoxic human vessels. This relationship ensured endothelium-dependent vasodilation under hypoxic conditions. Protective laminar blood flow restores eNOS expression and mitigates the hypoxic response on NOX4 and PTGIS.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"13 10","pages":""},"PeriodicalIF":6.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11504732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential Nitric Oxide Responses in Primary Cultured Keratinocytes and Fibroblasts to Visible and Near-Infrared Light. 原代培养的角质形成细胞和成纤维细胞对可见光和近红外线的一氧化氮反应差异。
IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-27 DOI: 10.3390/antiox13101176
Augustin C Barolet, Brice Magne, Daniel Barolet, Lucie Germain

NO is a crucial signaling molecule involved in skin health, the immune response, and the protection against environmental stressors. This study explores how different wavelengths of light, namely blue (455 nm), red (660 nm), and near infrared (NIR, 850 nm), affect nitric oxide (NO) production in skin cells. Primary keratinocytes and fibroblasts from three donors were exposed to these wavelengths, and NO production was quantified using a DAF-FM fluorescent probe. The results demonstrated that all three wavelengths stimulated NO release, with blue light showing the most pronounced effect. Specifically, blue light induced a 1.7-fold increase in NO in keratinocytes compared to red and NIR light and a 2.3-fold increase in fibroblasts compared to red light. Notably, fibroblasts exposed to NIR light produced 1.5 times more NO than those exposed to red light, while keratinocytes consistently responded more robustly across all wavelengths. In conclusion, blue light significantly boosts NO production in both keratinocytes and fibroblasts, making it the most effective wavelength. Red and NIR light, while less potent, also promote NO production and could serve as complementary therapeutic options, particularly for minimizing potential photoaging effects.

一氧化氮是参与皮肤健康、免疫反应和抵御环境压力的重要信号分子。本研究探讨了不同波长的光,即蓝光(455 nm)、红光(660 nm)和近红外线(NIR,850 nm)如何影响皮肤细胞中一氧化氮(NO)的产生。将来自三位供体的原代角质细胞和成纤维细胞暴露在这些波长下,并使用 DAF-FM 荧光探针对一氧化氮的产生进行量化。结果表明,三种波长的光都能刺激一氧化氮的释放,其中蓝光的效果最明显。具体来说,与红光和近红外光相比,蓝光诱导角质细胞中的 NO 增加了 1.7 倍,与红光相比,成纤维细胞中的 NO 增加了 2.3 倍。值得注意的是,暴露在近红外光下的成纤维细胞产生的 NO 是暴露在红光下的成纤维细胞的 1.5 倍,而角质细胞在所有波长下的反应都更为强烈。总之,蓝光能显著促进角质细胞和成纤维细胞产生 NO,是最有效的波长。红光和近红外光虽然效力较弱,但也能促进氮氧化物的产生,可以作为补充治疗方案,尤其是在最大限度地减少潜在的光老化效应方面。
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引用次数: 0
Bufotalin Induces Oxidative Stress-Mediated Apoptosis by Blocking the ITGB4/FAK/ERK Pathway in Glioblastoma. 布福他林通过阻断胶质母细胞瘤的 ITGB4/FAK/ERK 通路诱导氧化应激介导的细胞凋亡
IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-27 DOI: 10.3390/antiox13101179
Junchao Tan, Guoqiang Lin, Rui Zhang, Yuting Wen, Chunying Luo, Ran Wang, Feiyun Wang, Shoujiao Peng, Jiange Zhang

Bufotalin (BT), a major active constituent of Chansu, has been found to possess multiple pharmacological activities. Although previous studies have shown that BT could inhibit the growth of glioblastoma (GBM), the safety of BT in vivo and the potential mechanism are still unclear. We conducted a systematic assessment to investigate the impact of BT on GBM cell viability, migration, invasion, and colony formation. Furthermore, in vivo results were obtained to evaluate the effect of BT on tumor growth. The preliminary findings of our study demonstrate the effective inhibition of GBM cell growth and subcutaneous tumor development in mice by BT, with tolerable levels of tolerance observed. Mechanistically, BT treatment induced mitochondrial dysfunction, bursts of reactive oxygen species (ROS), and subsequent cell apoptosis. More importantly, proteomic-based differentially expressed proteins analysis revealed a significant downregulation of integrin β4 (ITGB4) following BT treatment. Furthermore, our evidence suggested that the ITGB4/focal adhesion kinase (FAK)/extracellular signal-related kinase (ERK) pathway involved BT-induced apoptosis. Overall, our study demonstrates the anti-GBM effects of BT and elucidates the underlying mechanism, highlighting BT as a potential therapeutic option for GBM.

布福他林(BT)是蟾酥的一种主要活性成分,具有多种药理活性。虽然以前的研究表明 BT 可以抑制胶质母细胞瘤(GBM)的生长,但 BT 在体内的安全性和潜在机制仍不清楚。我们进行了一项系统评估,研究 BT 对 GBM 细胞活力、迁移、侵袭和集落形成的影响。此外,我们还获得了体内结果,以评估 BT 对肿瘤生长的影响。我们的初步研究结果表明,BT 能有效抑制小鼠 GBM 细胞的生长和皮下肿瘤的形成,并能观察到可耐受的程度。从机理上讲,BT 治疗可诱导线粒体功能障碍、活性氧(ROS)爆发以及随后的细胞凋亡。更重要的是,基于蛋白质组学的差异表达蛋白分析表明,BT 治疗后整合素 β4(ITGB4)显著下调。此外,我们的证据表明,ITGB4/病灶粘附激酶(FAK)/细胞外信号相关激酶(ERK)通路参与了 BT 诱导的细胞凋亡。总之,我们的研究证明了 BT 的抗 GBM 作用,并阐明了其潜在机制,突出表明 BT 是治疗 GBM 的一种潜在选择。
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引用次数: 0
Modulatory Impact of Oxidative Stress on Action Potentials in Pathophysiological States: A Comprehensive Review. 氧化应激对病理生理状态下动作电位的调节作用:全面回顾。
IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-26 DOI: 10.3390/antiox13101172
Chitaranjan Mahapatra, Ravindra Thakkar, Ravinder Kumar

Oxidative stress, characterized by an imbalance between the production of reactive oxygen species (ROS) and the body's antioxidant defenses, significantly affects cellular function and viability. It plays a pivotal role in modulating membrane potentials, particularly action potentials (APs), essential for properly functioning excitable cells such as neurons, smooth muscles, pancreatic beta cells, and myocytes. The interaction between oxidative stress and AP dynamics is crucial for understanding the pathophysiology of various conditions, including neurodegenerative diseases, cardiac arrhythmias, and ischemia-reperfusion injuries. This review explores how oxidative stress influences APs, focusing on alterations in ion channel biophysics, gap junction, calcium dynamics, mitochondria, and Interstitial Cells of Cajal functions. By integrating current research, we aim to elucidate how oxidative stress contributes to disease progression and discuss potential therapeutic interventions targeting this interaction.

氧化应激的特点是活性氧(ROS)的产生与机体抗氧化防御之间的不平衡,它严重影响着细胞的功能和活力。它在调节膜电位,尤其是动作电位(AP)方面起着关键作用,而动作电位对神经元、平滑肌、胰腺β细胞和肌细胞等可兴奋细胞的正常功能至关重要。氧化应激与动作电位动力学之间的相互作用对于理解神经退行性疾病、心律失常和缺血再灌注损伤等各种疾病的病理生理学至关重要。这篇综述探讨了氧化应激如何影响 AP,重点是离子通道生物物理学、缝隙连接、钙动力学、线粒体和 Cajal 间质细胞功能的改变。通过整合当前的研究,我们旨在阐明氧化应激是如何导致疾病进展的,并讨论针对这种相互作用的潜在治疗干预措施。
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引用次数: 0
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Antioxidants
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