Anaerobe最新文献

英文中文

A metabolite dehydrogenase pathway represses sporulation of Clostridioides difficile 一种代谢物脱氢酶途径抑制艰难梭菌的产孢。

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-06-01 Epub Date: 2025-05-09 DOI: 10.1016/j.anaerobe.2025.102971

Daniela Wetzel , Arshad Rizvi , Adrianne N. Edwards , Shonna M. McBride

Objectives

Clostridioides difficile is a major gastrointestinal pathogen that is transmitted as a dormant spore. As an intestinal pathogen, C. difficile must contend with variable environmental conditions, including fluctuations in pH and nutrient availability. Nutrition and pH both influence growth and spore formation, but how pH and nutrition jointly influence sporulation are not known. In this study, we investigated the dual impact of pH and pH-dependent metabolism on C. difficile sporulation.

Methods

We examined the impacts of pH and the metabolite acetoin on C. difficile growth, gene expression, and sporulation.

Results

We found that expression of the predicted acetoin dehydrogenase operon, CD0035-CD0039, was pH-dependent and repressed by acetoin and pyruvate. Regulation of the C. difficile CD0035-CD0039 locus is distinct from characterized orthologous systems and appears to involve a co-transcribed DeoR-family regulator, rather than a sigma⁵⁴-dependent activator. In addition, an CD0036 null mutant produced significantly more spores and initiated sporulation earlier than the parent strain. However, unlike other Firmicutes, growth and culture density of C. difficile was not increased by acetoin availability or disruption of the dehydrogenase pathway.

Conclusions

Together, these results indicate that acetoin, pH, and the CD0036-CD0039 dehydrogenase pathway play important roles in nutritional repression of sporulation in C. difficile. However, the data do not support the involvement of the CD0036-CD0039 pathway in acetoin metabolism and acetoin is not a significant stationary phase energy source for C. difficile.

艰难梭菌是一种主要的胃肠道病原体，通过休眠孢子传播。作为一种肠道病原体，艰难梭菌必须适应多变的环境条件，包括pH值和养分有效性的波动。营养和pH都影响生长和孢子形成，但pH和营养如何共同影响孢子形成尚不清楚。目的：在本研究中，我们研究了pH和pH依赖性代谢对艰难梭菌产孢的双重影响。方法：我们检测了pH和代谢物乙酰胆碱对艰难梭菌生长、基因表达和产孢的影响。结果：我们发现预测的乙酰丙酮脱氢酶操纵子CD0035-CD0039的表达是ph依赖性的，并被乙酰丙酮酸和丙酮酸抑制。艰难梭菌CD0035-CD0039位点的调控不同于特征的同源系统，似乎涉及共转录的deor家族调控因子，而不是依赖sigma54的激活因子。此外，CD0036零突变体比亲本菌株产生更多孢子和更早开始孢子形成。然而，与其他厚壁菌门不同的是，艰难梭菌的生长和培养密度不会因乙酰托因的可用性或脱氢酶途径的破坏而增加。结论：综上所述，acetoin、pH和CD0036-CD0039脱氢酶途径在艰难梭菌芽孢的营养抑制中起重要作用。然而，这些数据并不支持CD0036-CD0039途径参与乙酰托因代谢，并且乙酰托因不是艰难梭菌的重要固定相能量来源。

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引用次数: 0

Development of a polymicrobial host-bacterial interface biofilm model for bacterial vaginosis 细菌性阴道病多微生物宿主-细菌界面生物膜模型的建立。

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-06-01 Epub Date: 2025-03-08 DOI: 10.1016/j.anaerobe.2025.102952

William Johnston, Ryan Kean

Bacterial vaginosis (BV) is characterised by a polymicrobial biofilm forming on the vaginal epithelium. In this study, we have developed a host-pathogen model of BV to replicate disease. We demonstrated tissue colonisation by four key vaginal pathobionts that formed metronidazole tolerant biofilms, with subtle changes in cytotoxicity and inflammation.

细菌性阴道病（BV）的特征是在阴道上皮上形成多微生物生物膜。在这项研究中，我们建立了一个BV的宿主-病原体模型来复制疾病。我们证明了四种关键的阴道病原体形成甲硝唑耐受性生物膜的组织定植，在细胞毒性和炎症方面有细微的变化。

引用次数: 0

Molecular detection of toxigenic Clostridioides difficile in subgingival biofilm of severe periodontitis 重度牙周炎龈下生物膜中产毒艰难梭菌的分子检测。

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-06-01 Epub Date: 2025-03-15 DOI: 10.1016/j.anaerobe.2025.102955

Isabela Leite de Oliveira Rosa, Eliane de Oliveira Ferreira, Ana Paula Vieira Colombo

Objectives

The oral cavity is the main gateway for the entry of C. difficile spores to the digestive tract. In conditions of poor oral hygiene and periodontal diseases, the dysbiotic oral microbiota may be a reservoir for several human pathogens. Here, we explored the prevalence of C. difficile in the oral microbiota of patients with severe periodontitis by the molecular detection of species specific genes.

Methods

Subgingival biofilm, saliva and/or feces from 659 patients with gingivitis, periodontitis and no periodontal diseases were screened for the tpi and toxin A/B genes specific for C. difficile by multiplex PCR. Differences among groups were sought by the Chi-square test.

Results

The overall frequency of C. difficile tpi gene was 29 %, with a high detection of tcdB gene (44.8 %). Patients with periodontitis showed a greater prevalence of this gene in the biofilm than individuals with gingivitis and periodontal health (p = 0.001), particularly at more severe stages of disease (p < 0.05). No toxin genes were detected in feces or biofilm from healthy patients, whereas >70 % of the biofilm from patients with periodontal diseases were positive for these genes (p < 0.001). Detection of C. difficile tpi gene in oral/fecal samples correlated with periodontal inflammation (p < 0.05). A modest intra-individual agreement between tpi gene detection in feces and saliva was found within periodontitis patients (Kappa = 0.314; p = 0.003).

Conclusion

The high frequency of the C. difficile specific genes tpi and tcdB in the dysbiotic subgingival biofilm of advanced periodontitis could support the presence of the bacterium in this niche.

目的：口腔是艰难梭菌孢子进入消化道的主要通道。在口腔卫生不良和牙周病的情况下，口腔微生物群可能是几种人类病原体的储存库。在这里，我们通过物种特异性基因的分子检测来探讨艰难梭菌在严重牙周炎患者口腔微生物群中的流行情况。方法：对659例牙龈炎、牙周炎和非牙周病患者的龈下生物膜、唾液和/或粪便进行多重PCR筛选，检测难辨梭菌特异性tpi和毒素A/B基因。通过卡方检验寻找组间差异。结果：难辨梭菌tpi基因总检出率为29%，其中tcdB基因检出率较高（44.8%）。与牙龈炎和牙周健康的个体相比，牙周炎患者在生物膜中显示出更高的该基因的患病率（p=0.001），特别是在疾病的更严重阶段（牙周病患者的生物膜中有70%的这些基因呈阳性）。结论：晚期牙周炎患者牙龈下生物膜中难辨梭菌特异性基因tpi和tcdB的高频率可能支持该物种在该生态位中的存在。

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引用次数: 0

Antimicrobial resistance profiles of Clostridium species isolated from post-traumatic infections in a Costa Rican hospital 哥斯达黎加一家医院创伤后感染中分离的梭状芽孢杆菌的抗微生物药物耐药性谱

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-06-01 Epub Date: 2025-03-24 DOI: 10.1016/j.anaerobe.2025.102957

Mariela Alvarado-Rodríguez , Carlos Quesada-Gómez

This study analyzed antimicrobial resistance in 119 Clostridium species isolates collected at a Costa Rican trauma hospital (2018–2022). Isolates were identified using MALDI-TOF, and antimicrobial susceptibility was assessed by E-test. C. perfringens, C. tertium, and Paeniclostridium sordellii were most prevalent species. All isolates were susceptible to amoxicillin-clavulanic acid, linezolid, and metronidazole; however, resistance rates of 20 % to vancomycin and 25 % to clindamycin were observed. C. sphenoides showed the highest minimum inhibitory concentration values. Based on these findings, empirical treatment strategies at the hospital now prioritize amoxicillin-clavulanic acid, linezolid, and metronidazole, while reducing reliance on clindamycin due to observed resistance patterns.

本研究分析了哥斯达黎加一家创伤医院（2018-2022）收集的119种梭菌的抗微生物药物耐药性。采用MALDI-TOF对分离菌株进行鉴定，并用E-test对其进行药敏试验。产气荚膜梭菌、特氏梭菌和索氏小梭菌是最常见的菌种。所有分离株均对阿莫西林-克拉维酸、利奈唑胺和甲硝唑敏感；万古霉素耐药率为20%，克林霉素耐药率为25%。最小抑菌浓度最高。基于这些发现，医院的经验治疗策略现在优先考虑阿莫西林-克拉维酸、利奈唑胺和甲硝唑，同时由于观察到耐药模式而减少对克林霉素的依赖。

引用次数: 0

Novel competitive annealing-mediated isothermal amplification (CAMP)-based detection of epsilon toxin gene in Clostridium perfringens 基于竞争退火介导的等温扩增（CAMP）检测产气荚膜梭菌epsilon毒素基因的新方法。

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-06-01 Epub Date: 2025-03-26 DOI: 10.1016/j.anaerobe.2025.102958

A. Arun Prince Milton , Torik Basar , K. Srinivas , Aleimo G. Momin , Sabia Khan , G. Bhuvana Priya , Samir Das , S. Ghatak , K. Puro

In this study, we developed a novel competitive annealing-mediated isothermal amplification (CAMP)-based assay for the detection of the etx gene, which encodes the epsilon toxin of Clostridium perfringens. The assay, performed at 60 °C for 60 min, demonstrated 100 % specificity and was 100 times more sensitive than endpoint PCR.

在这项研究中，我们开发了一种新的基于竞争退火介导的等温扩增（CAMP）的检测方法，用于检测产气荚膜梭菌（Clostridium perfringens）中编码epsilon毒素的etx基因。实验在60°C下进行60分钟，显示出100%的特异性，比终点PCR灵敏100倍。

引用次数: 0

Genomic analysis and mobile genetic elements carriage of Clostridium perfringens type A A型产气荚膜梭菌基因组分析及可移动遗传元件携带。

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-06-01 Epub Date: 2025-05-03 DOI: 10.1016/j.anaerobe.2025.102970

Yajun Jiang , Haoran Zheng , Lulu Bai, Yuanyuan Wang, Jiaxin Zhong, Wenzhu Zhang, Telong Xu, Yuanhang Pan, Jingjing Tang, Jinxing Lu, Bike Zhang, Yuan Wu

Objectives

To explore a comprehensive genomic analysis of Clostridium perfringens type A strains from diverse regions in China, investigating their virulence genes, antibiotic resistance genes, and mobile genetic elements (MGEs) to inform strategies for infection control and resistance gene surveillance.

Methods

We conducted whole-genome sequencing on 168 C. perfringens type A strains from nine provinces in China (2016–2021). Previously described alpha-toxin (PLC) sequence typing for C. perfringens was used for comparisons with core genome multilocus sequence typing. Virulence genes, antibiotic resistance genes, and MGEs, including CRISPR/Cas, prophages, and plasmids of C. perfringens type A were investigated by molecular and bioinformatic methods.

Results

PLC type II contained the largest number of isolates (n = 44). The same type strains were largely clustered in the same branches. Tetracycline resistance genes tetA(P) and tetB(P) had high prevalence in type A isolates. 395 prophages were predicted including 265 “incomplete,” 55 “questionable,” and 75 “intact” prophages. CRISPR/Cas systems were more common in isolates from humans (63 %) than in those from animals and food (52 % and 46 %, respectively). Fifty-seven percent of strains likely had the tcp conjugation locus (tcpC to tcpH), and 12 isolates likely carried the conjugative pCW3 plasmid. Type A strains exhibited fewer plasmid-encoded toxins.

Conclusions

cgMLST analysis demonstrated some micro-evolution and regional transmission trends within type A, which exhibited partial correlated with PLC typing. This study highlights the need for enhanced surveillance of antimicrobial resistance and pathogenicity-associated MGEs in C. perfringens type A.

目的：对中国不同地区产气荚膜梭菌a型菌株进行基因组分析，了解其毒力基因、抗生素耐药基因和移动遗传元件（MGEs），为感染控制和耐药基因监测提供依据。方法：对2016-2021年中国9省168株A型产气荚膜荚膜杆菌进行全基因组测序。利用先前描述的产气荚膜荚膜荚膜菌α毒素（PLC）序列分型与核心基因组多位点序列分型进行比较。采用分子和生物信息学方法研究了产气荚膜荚膜菌A型的毒力基因、耐药基因和MGEs，包括CRISPR/Cas、噬菌体和质粒。结果：PLC II型菌株数量最多（n=44）；同一类型菌株大多聚集在同一枝上。四环素耐药基因tetA(P)和tetB(P)在A型分离株中流行率较高。预计有395个噬菌体，包括265个“不完整”，55个“可疑”和75个“完整”的噬菌体。CRISPR/Cas系统在人类分离株（63%）中比在动物和食物分离株（分别为52%和46%）中更常见。57%的菌株可能具有tcp偶联位点（tcpC到tcpH）， 12株菌株可能携带偶联pCW3质粒。A型菌株表现出较少的质粒编码毒素。结论：cgMLST分析显示A型病毒有一定的微观演化和区域传播趋势，与PLC分型部分相关。本研究强调了加强对A型产气荚膜荚膜杆菌抗菌素耐药性和致病性相关MGEs监测的必要性。

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引用次数: 0

Culture performance of Fastidious Anaerobe Agar for frequently-encountered anaerobic bacteria, and potential application in EUCAST antimicrobial susceptibility testing 厌氧琼脂对常见厌氧菌的培养性能及其在EUCAST抗菌药敏试验中的应用前景。

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-06-01 Epub Date: 2025-05-19 DOI: 10.1016/j.anaerobe.2025.102972

Katrine Agerbo Hovmand , Ziyap Acar , Emil Ainsworth Jochumsen , Sanne Malig , Jens Kjeldsen , Dorte Kinggaard Holm , Anna Christine Nilsson , Torkell Ellingsen , Maja Skov Kragsnaes , Ulrik Stenz Justesen

The culture performance of Fastidious Anaerobe Agar (FAA-HB) was evaluated for fecal samples and candidate-species for EUCAST antimicrobial susceptibility testing (AST). Thirty-six anaerobic species were cultured from fecal samples and most EUCAST candidate-species exhibited confluent growth within 16–20 h. The FAA-HB performed excellent for both culture and AST of a broad spectrum of anaerobic bacteria.

研究了厌氧琼脂（FAA-HB）在粪便样品和EUCAST药敏试验候选菌种中的培养性能。从粪便样品中培养36种厌氧菌，大多数EUCAST候选菌在16-20小时内表现出融合生长。FAA-HB对广谱厌氧菌的培养和AST均表现优异。

引用次数: 0

Growth of hydrogenotrophic methanogen Methanoculleus bourgensis MAB1 in the presence of dunite 养氢甲烷菌 Methanoculleus bourgensis MAB1 在白云石存在下的生长。

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-04-01 Epub Date: 2025-02-26 DOI: 10.1016/j.anaerobe.2025.102945

A. Neubeck , N. Callac , S. Isaksson , A. Schnürer

This study investigated the long-term potential of low-temperature serpentinization of dunite to support the growth of the hydrogenotrophic methanogen Methanoculleus bourgensis strain MAB1. Incubation experiments were conducted for over 800 days, using dunite powder as the sole energy source, with and without the addition of nickel, an essential cofactor for methanogenesis. The results indicated that hydrogen released from dunite was sufficient to sustain methanogen growth, but the process was slow, with methane production beginning only after approximately 300 days. The release of toxic metals from dunite, particularly zinc, appeared to inhibit methanogen growth over time, leading to the cessation of methane production after 528 days and likely the lysing of the methanogenic cells. The study suggests that hydrogen availability, rather than nickel, is the limiting factor for methanogen growth in these conditions.

本研究探讨了低温长蛇纹岩化对支持富氢产甲烷菌MAB1生长的长期潜力。在800多天的孵育实验中，使用硬岩粉作为唯一的能源，添加和不添加镍（甲烷生成的重要辅助因子）。结果表明，从泥质岩中释放的氢足以维持产甲烷菌的生长，但这一过程缓慢，大约300天后才开始产甲烷。随着时间的推移，从坚岩中释放的有毒金属，特别是锌，似乎抑制了产甲烷菌的生长，导致528天后甲烷生产停止，产甲烷细胞可能会裂解。研究表明，在这些条件下，氢的可用性，而不是镍，是甲烷菌生长的限制因素。

引用次数: 0

A retrospective study comparing the effectiveness of carbapenems and tazobactam/piperacillin as an empirical treatment for patients infected with Bacteroides fragilis 碳青霉烯类药物与他唑巴坦/哌拉西林治疗脆弱拟杆菌感染的回顾性研究

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-04-01 Epub Date: 2025-03-01 DOI: 10.1016/j.anaerobe.2025.102950

Hideo Kato , Mao Hagihara , Nobuhiro Asai , Hiroshige Mikamo , Takuya Iwamoto

Background

Bacteroides fragilis is one of the most frequently isolated pathogenic anaerobic bacteria. Infections caused by B. fragilis are commonly treated with carbapenems or tazobactam/piperacillin. However, there is no evidence for the optimal antibiotic choice against infections caused by B. fragilis as an empirical therapy owing to its varied antibiotic resistance mechanisms. Hence, we compared the effectiveness of carbapenems and tazobactam/piperacillin as an antibiotic therapy in patients infected with B. fragilis.

Methods

We investigated mortality, clinical and antimicrobial efficacy, and the percentage of patients who switched to broad-spectrum antibiotics, such as carbapenems or tazobactam/piperacillin, due to the aggravated symptoms of infection in patients receiving carbapenems or tazobactam/piperacillin from 2019 to 2024.

Results

A total of 60 patients were included in the study; 24 patients received carbapenems and 36 received tazobactam-piperacillin as an empirical treatment against B. fragilis infections. None of the patients in either group died. Compared to carbapenem treatment, tazobactam/piperacillin treatment significantly improved inflammatory markers, including body temperature and C-reactive protein (45.8 % vs. 72.2 %, p = 0.039 and 37.5 % vs. 63.4 %, p = 0.045, respectively; carbapenem vs. tazobactam/piperacillin). The percentage of patients switching from carbapenems to tazobactam/piperacillin or vice versa was significantly lower in the tazobactam/piperacillin group than in the carbapenem group (41.7 % vs. 11.1 %, p < 0.001).

Conclusions

Our findings suggest that tazobactam/piperacillin can be used as the first-line empirical treatment for patients infected with B. fragilis.

背景：脆弱拟杆菌是最常见的致病性厌氧菌之一。易裂芽孢杆菌引起的感染通常用碳青霉烯类或他唑巴坦/哌拉西林治疗。然而，由于脆弱芽孢杆菌的多种抗生素耐药机制，尚无证据表明针对脆弱芽孢杆菌感染的最佳抗生素选择是一种经验疗法。因此，我们比较了碳青霉烯类和他唑巴坦/哌拉西林作为抗生素治疗脆弱杆菌感染患者的有效性。方法：研究2019年至2024年期间，由于碳青霉烯类或他唑巴坦/哌拉西林感染症状加重，患者改用碳青霉烯类或他唑巴坦/哌拉西林等广谱抗生素的死亡率、临床和抗菌效果，以及患者比例。结果：共纳入60例患者；24例患者接受碳青霉烯类药物治疗，36例接受他唑巴坦-哌拉西林治疗。两组患者均未死亡。与碳青霉烯治疗相比，他唑巴坦/哌西林治疗显著改善炎症标志物，包括体温和c反应蛋白（分别为45.8%比72.2%,p = 0.039和37.5%比63.4%,p = 0.045）；碳青霉烯与他唑巴坦/哌拉西林)。他唑巴坦/哌拉西林组患者从碳青霉烯类药物转向他唑巴坦/哌拉西林或反之的比例显著低于碳青霉烯类药物组（41.7% vs. 11.1%, p < 0.001）。结论：他唑巴坦/哌拉西林可作为脆弱贝氏杆菌感染的一线经验治疗方案。

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引用次数: 0

Description of three new Leptotrichia species isolated from dental biofilm: Leptotrichia rugosa sp. nov., Leptotrichia mesophila sp. nov. and Leptotrichia alba sp. nov. 描述从牙科生物膜中分离出的三个新的 Leptotrichia 物种：Leptotrichia rugosa sp.

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-04-01 Epub Date: 2025-02-25 DOI: 10.1016/j.anaerobe.2025.102948

Xia Wang , Shi-Yan Jiao , Jie Wang, Ran-Ran Wu, Tong-Tong Zhang, Chun-Miao Wang, Xiao-Jun Li

Three bacterial strains, namely HSP-334^T, HSP-342^T and HSP-536^T, were isolated from human oral dental biofilm. These strains were identified as Gram stain-negative, straight or slightly curved anaerobes. Based on 16S rRNA genes analysis, strain HSP-334^T exhibited the closest identity to Leptotrichia shahii LB37^T (92.25 %). Strain HSP-342^T demonstrated the highest similarity to Leptotrichia hongkongensis HKU24^T (98.03 %), while strain HSP-536^T displayed the greatest resemblance to Leptotrichia buccalis DSM 1135^T (97.77 %). Notably, the maximum sequence similarity among the three isolates ranged from 91.56 % to 94.12 %. All the phylogenies showed that strains HSP-334^T, HSP-342^T, HSP-536^T, all members of genus Leptotrichia and Pseudoleptotrichia goodfellowii JCM 16774^T were clustered in one subclade within the family Leptotrichiaceae. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated between these three strains and their phylogenetically related species were determined to be lower than the established species delineation threshold values. The major cellular fatty acids detected in these novel strains were C_16:0 and C_18:1 ω7c. Strains HSP-334^T, HSP-342^T and HSP-536^T could be distinguished from each other by several phenotypic characteristics. Based on the comprehensive polyphasic taxonomic characterizations conducted, strains HSP-334^T, HSP-342^T and HSP-536^T represent three novel species of the genus Leptotrichia, for which the name Leptotrichia rugosa sp. nov. (type strain HSP-334^T = JCM 36566^T = CGMCC 1.18095^T = MCCC 1K09354^T), Leptotrichia mesophila sp. nov. (type strain HSP-342^T = JCM 36567^T = CGMCC 1.18052^T = MCCC 1K09338^T) and Leptotrichia alba sp. nov. (type strain HSP-536^T = JCM 36662^T = CGMCC 1.18096^T = MCCC 1K09339^T) are proposed.

从人口腔生物膜中分离到3株细菌，分别为HSP-334T、HSP-342T和HSP-536T。这些菌株被鉴定为革兰氏阴性，直或微弯曲厌氧菌。基于16S rRNA基因分析，菌株HSP-334T与沙希细毛菌LB37T的同源性最接近（92.25%）。菌株HSP-342T与香港钩毛菌HKU24T相似度最高（98.03%），菌株HSP-536T与颊钩毛菌DSM 1135T相似度最高（97.77%）。值得注意的是，3个分离株的最大序列相似性在91.56% ~ 94.12%之间。所有系统发育结果表明，菌株HSP-334T、HSP-342T、HSP-536T、细毛菌属和假细毛菌JCM 16774T均聚集在细毛菌科的一个亚枝上。结果表明，这3株菌株及其亲缘种的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值均低于已建立的种划分阈值。在这些新菌株中检测到的主要细胞脂肪酸为C16:0和C18:1ω7c。菌株HSP-334T、HSP-342T和HSP-536T可以通过几个表型特征进行区分。综合多相分类鉴定结果表明，菌株HSP-334T、HSP-342T和HSP-536T代表细毛菌属的3个新种，其中命名为细毛菌（Leptotrichia rugosa sp. nov.）（型菌株HSP-334T = JCM 36566T = CGMCC 1.18095T = MCCC 1K09354T）；提出了嗜中毛纤毛菌11 .（型菌株HSP-342T = JCM 36567T = CGMCC 1.18052T = MCCC 1K09338T）和白毛纤毛菌11 .（型菌株HSP-536T = JCM 36662T = CGMCC 1.18096T = MCCC 1K09339T）。

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