Pub Date : 2020-01-01DOI: 10.36648/2386-5180.8.1.310
Nnadozie Igbokwe, D. Jeyanesan, A. Mehta
We report a case of live cervical ectopic pregnancy (CEP) at 6 weeks gestation. A 36-year-old nulliparous who presented with mild bleeding in vagina. She was haemodynamic ally stable with a serum B-hCG of 10713. A transvaginal ultrasound scan showed an empty uterus and lives cervical ectopic pregnancy with a fetal pole measuring 7.1 mm and yolk sac with negative sliding sign. She was counselled on options of management and had hysteroscopy and surgical evacuation under ultrasound guidance with no complications and post-operative methotrexate injection. She had a significant drop in her serial B-hCG and urine pregnancy test 3 weeks after surgery was negative.
{"title":"Management of Live Cervical Ectopic Pregnancy","authors":"Nnadozie Igbokwe, D. Jeyanesan, A. Mehta","doi":"10.36648/2386-5180.8.1.310","DOIUrl":"https://doi.org/10.36648/2386-5180.8.1.310","url":null,"abstract":"We report a case of live cervical ectopic pregnancy (CEP) at 6 weeks gestation. A 36-year-old nulliparous who presented with mild bleeding in vagina. She was haemodynamic ally stable with a serum B-hCG of 10713. A transvaginal ultrasound scan showed an empty uterus and lives cervical ectopic pregnancy with a fetal pole measuring 7.1 mm and yolk sac with negative sliding sign. She was counselled on options of management and had hysteroscopy and surgical evacuation under ultrasound guidance with no complications and post-operative methotrexate injection. She had a significant drop in her serial B-hCG and urine pregnancy test 3 weeks after surgery was negative.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79479787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.36648/2386-5180.8.1.308
Nnadozie Igbokwe, M. Tomlinson
Study question: Can the use of radio frequency identification (RFID) and track and trace technology reduce the risks and cost of sperm cryopreservation? Summary answer: The use this system is more efficient and accurate with no biosafety issue when compared with the manual method. What is known: The use of the manual system for auditing of cryopreserved samples is tedious and expensive, with high rate of mix-up in ART settings. No documented evidence about any biohazard effect of high frequency RFID tags (13.56 MHz) on gametes and embryos. Study design: A prospective study cohort study using 20 sperm samples. Only samples with initial good quality were used, and the study lasted for 6 weeks. Methods: Reliability of the system was evaluated by doing multiple reads and percentage accuracy recorded. The prepared samples were exposed to continuous RFID radiation over 24 hours and their motility and speed checked serially using computerised assisted semen analysis (CASA). Comparison was made with a control group and secondarily with samples on heated block at 36°C. Statistical analysis was done between the groups. Results: Reliability of 100% as a witnessing system was practically recorded. No significant effect of RFID radiation on sperm motility, however increased temperature significantly reduced both sperm motility and velocity with time (p<0.0001). Limitations: Small sample size of 20. Technical errors and challenges of a new system affected extensive biosafety evaluation Wider implication: Better structured studies are needed to confirm these findings
{"title":"The Use of Radio Frequency Identification (RFID) and Track and Trace Technology in Reducing the Risks and Cost of Sperm Cryopreservation","authors":"Nnadozie Igbokwe, M. Tomlinson","doi":"10.36648/2386-5180.8.1.308","DOIUrl":"https://doi.org/10.36648/2386-5180.8.1.308","url":null,"abstract":"Study question: Can the use of radio frequency identification (RFID) and track and trace technology reduce the risks and cost of sperm cryopreservation? Summary answer: The use this system is more efficient and accurate with no biosafety issue when compared with the manual method. What is known: The use of the manual system for auditing of cryopreserved samples is tedious and expensive, with high rate of mix-up in ART settings. No documented evidence about any biohazard effect of high frequency RFID tags (13.56 MHz) on gametes and embryos. Study design: A prospective study cohort study using 20 sperm samples. Only samples with initial good quality were used, and the study lasted for 6 weeks. Methods: Reliability of the system was evaluated by doing multiple reads and percentage accuracy recorded. The prepared samples were exposed to continuous RFID radiation over 24 hours and their motility and speed checked serially using computerised assisted semen analysis (CASA). Comparison was made with a control group and secondarily with samples on heated block at 36°C. Statistical analysis was done between the groups. Results: Reliability of 100% as a witnessing system was practically recorded. No significant effect of RFID radiation on sperm motility, however increased temperature significantly reduced both sperm motility and velocity with time (p<0.0001). Limitations: Small sample size of 20. Technical errors and challenges of a new system affected extensive biosafety evaluation Wider implication: Better structured studies are needed to confirm these findings","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78081369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.36648/2386-5180.8.1.309
P. Nobis, Himleena Gautam, K. Kathar, P. Goswami, Joydev Sharma
Background: Physiological changes in spiral arteries i.e. remodeling occur upto myometrial segment of the vessels and these changes are important for growth of the fetus. However in hypertensive pregnancies and IUGR, these changes are often not seen and arteries remain non-dilated. The aims of the study were to assess the extent of spiral vessel dilatation in hypertensive pregnancies and IUGR cases. Methods: Placental bed biopsy were taken in cases who underwent caesarean section and gave consent for the study, during the study period. 50 cases were included. Morphology and diameters of spiral arteries were assessed by histopathological examination. Arteries who retained the non-pregnant morphology were considered non-dilated and those who showed features of remodeling were considered normal. 14 cases were excluded as no vessel was seen in biopsy specimen. T-test and Chi-square tests were used for analysis. Out of the included 36 cases, percentage of non-dilated vessels was significantly high in gestational hypertension and preeclampsia(p=0.0230) and also significantly high in IUGR cases, (p=0.0113),irrespective of hypertension. Also the percentage of non-dilated vessels was higher in nulliparous women, though not significant. Placental bed biopsy specimens have limitations because they only provide information about a small segment of the placental bed. It is possible that areas close to the nonbiopsy site may have a completely different degree of vascular transformation. Moreover the number of cases in this study is less. Conclusion: Absence of spiral arterial remodeling is a crucial factor in hypertension in pregnancy and low birth weight babies. And adequate research is needed in this aspect so that such knowledge can help us to prevent hypertension in pregnancy and IUGR.
{"title":"Spiral Arterial Morphology in Relation to Hypertension in Pregnancy and Birth Weight by Placental Bed Biopsy: A Retrospective Analysis","authors":"P. Nobis, Himleena Gautam, K. Kathar, P. Goswami, Joydev Sharma","doi":"10.36648/2386-5180.8.1.309","DOIUrl":"https://doi.org/10.36648/2386-5180.8.1.309","url":null,"abstract":"Background: Physiological changes in spiral arteries i.e. remodeling occur upto myometrial segment of the vessels and these changes are important for growth of the fetus. However in hypertensive pregnancies and IUGR, these changes are often not seen and arteries remain non-dilated. The aims of the study were to assess the extent of spiral vessel dilatation in hypertensive pregnancies and IUGR cases. Methods: Placental bed biopsy were taken in cases who underwent caesarean section and gave consent for the study, during the study period. 50 cases were included. Morphology and diameters of spiral arteries were assessed by histopathological examination. Arteries who retained the non-pregnant morphology were considered non-dilated and those who showed features of remodeling were considered normal. 14 cases were excluded as no vessel was seen in biopsy specimen. T-test and Chi-square tests were used for analysis. Out of the included 36 cases, percentage of non-dilated vessels was significantly high in gestational hypertension and preeclampsia(p=0.0230) and also significantly high in IUGR cases, (p=0.0113),irrespective of hypertension. Also the percentage of non-dilated vessels was higher in nulliparous women, though not significant. Placental bed biopsy specimens have limitations because they only provide information about a small segment of the placental bed. It is possible that areas close to the nonbiopsy site may have a completely different degree of vascular transformation. Moreover the number of cases in this study is less. Conclusion: Absence of spiral arterial remodeling is a crucial factor in hypertension in pregnancy and low birth weight babies. And adequate research is needed in this aspect so that such knowledge can help us to prevent hypertension in pregnancy and IUGR.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76923823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.36648/2386-5180.8.2.314
K. Sridevi, Amit Munjal, A. Ch, S. Nachiappan, P. Raman, S. Bhalla, Sourya Kumar, A. Nayyar
Humanity has witnessed a pandemic in late December or, early January. Novel corona virus nCov-19 is responsible for causing COVID-19. The first case was reported in Wuhan city, China in the month of December 2019. No specific drugs have been approved yet for its treatment, though, convalescent plasma (CP) therapy is expected to increase the survivable rate. The history of convalescent plasma therapy dates back to the early 20th century. A plethora of studies suggest that CP can be used to treat the emerging infectious diseases. We had a systematic search in PubMed and found numerous Chinese and Korean clinical trials of convalescent plasma transfusions. The present review gives an insight in to the same.
{"title":"Convalescent Plasma Therapy for Prophylaxis and Treatment of COVID-19: A Systematic Research of Facts and Files, A Narrative Review","authors":"K. Sridevi, Amit Munjal, A. Ch, S. Nachiappan, P. Raman, S. Bhalla, Sourya Kumar, A. Nayyar","doi":"10.36648/2386-5180.8.2.314","DOIUrl":"https://doi.org/10.36648/2386-5180.8.2.314","url":null,"abstract":"Humanity has witnessed a pandemic in late December or, early January. Novel corona virus nCov-19 is responsible for causing COVID-19. The first case was reported in Wuhan city, China in the month of December 2019. No specific drugs have been approved yet for its treatment, though, convalescent plasma (CP) therapy is expected to increase the survivable rate. The history of convalescent plasma therapy dates back to the early 20th century. A plethora of studies suggest that CP can be used to treat the emerging infectious diseases. We had a systematic search in PubMed and found numerous Chinese and Korean clinical trials of convalescent plasma transfusions. The present review gives an insight in to the same.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78355263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.36648/2386-5180.8.2.313
Nwaogwugwu Jc
Management of blood glucose level is the hallmark in the treatment of diabetes. Much work has not been done on the management of diabetes using the stem tuber extract of Colocasia esculenta. The objective of this study was to evaluate the antihyperglycemic and hematological parameter on Colocasia esculenta aqueous stem extract in alloxan induced diabetic rats. Sixty (60) male rats were used in the study. Seven days of acclimatization, the rats were divided randomly into six groups of five in each group. Group 1: Served as normal control, Group 2: Diabetic control group (negative control), Group 3: Diabetic group and “Glucinorm-M80” (positive control), Group 4: Diabetic group and extract at 200 mg/kg bodyweight, Group 5: Diabetic group and extract at 400 mg/kg, Group 6: Diabetic group and extract at 600 mg/kg. Diabetes was induced in albino rats by intraperitoneal injection of alloxan at a single dose of 120 mg/kg body weight in groups 2 to 6 after starving them for 24 hrs. The animals were given feed and water ad libitum. The albino rats were administered for twenty eight days with the aqueous Colocasia esculenta stem tuber, after which they were fasted overnight, anaesthetized with chloroform and sacrificed. The result showed that there was a significant increase (p<0.05) in mean body weight of the positive control and the treatment groups (200 mg/kg to 600 mg/kg) when compared with the negative control which has a significant decrease (p<0.05) in mean body weight. The result showed that there was a substantial decrease in blood glucose of animals administered with different doses of aqueous extract of Colocasia esculenta stem tuber and the positive control group. The result showed that a significant difference (P<0.05) in the concentrations of RBC, PCV, HB, PLT and MCH of the treatment groups and negative group when compared with the normal control group. Also there was a significant difference in glycosylate hemoglobin of the treatment groups when compared with the control group and negative group. This study has demonstrated that aqueous stem tuber extract of Colocasia esculenta has a significant increase on body weight which may have a role of improving the states of possible weight loss following complicated diabetes. Also, aqueous stem tuber extract of Colocasia esculenta has an ameliorative effect on sugar level and some hematological parameters of alloxan induced diabetic rats showing effective diabetic control and management of diabetes.
{"title":"Hematological changes and antidiabetic activities of Colocasia esculenta (L.schatt) stem tuber aqueous extract in alloxan induced diabetic rats","authors":"Nwaogwugwu Jc","doi":"10.36648/2386-5180.8.2.313","DOIUrl":"https://doi.org/10.36648/2386-5180.8.2.313","url":null,"abstract":"Management of blood glucose level is the hallmark in the treatment of diabetes. Much work has not been done on the management of diabetes using the stem tuber extract of Colocasia esculenta. The objective of this study was to evaluate the antihyperglycemic and hematological parameter on Colocasia esculenta aqueous stem extract in alloxan induced diabetic rats. Sixty (60) male rats were used in the study. Seven days of acclimatization, the rats were divided randomly into six groups of five in each group. Group 1: Served as normal control, Group 2: Diabetic control group (negative control), Group 3: Diabetic group and “Glucinorm-M80” (positive control), Group 4: Diabetic group and extract at 200 mg/kg bodyweight, Group 5: Diabetic group and extract at 400 mg/kg, Group 6: Diabetic group and extract at 600 mg/kg. Diabetes was induced in albino rats by intraperitoneal injection of alloxan at a single dose of 120 mg/kg body weight in groups 2 to 6 after starving them for 24 hrs. The animals were given feed and water ad libitum. The albino rats were administered for twenty eight days with the aqueous Colocasia esculenta stem tuber, after which they were fasted overnight, anaesthetized with chloroform and sacrificed. The result showed that there was a significant increase (p<0.05) in mean body weight of the positive control and the treatment groups (200 mg/kg to 600 mg/kg) when compared with the negative control which has a significant decrease (p<0.05) in mean body weight. The result showed that there was a substantial decrease in blood glucose of animals administered with different doses of aqueous extract of Colocasia esculenta stem tuber and the positive control group. The result showed that a significant difference (P<0.05) in the concentrations of RBC, PCV, HB, PLT and MCH of the treatment groups and negative group when compared with the normal control group. Also there was a significant difference in glycosylate hemoglobin of the treatment groups when compared with the control group and negative group. This study has demonstrated that aqueous stem tuber extract of Colocasia esculenta has a significant increase on body weight which may have a role of improving the states of possible weight loss following complicated diabetes. Also, aqueous stem tuber extract of Colocasia esculenta has an ameliorative effect on sugar level and some hematological parameters of alloxan induced diabetic rats showing effective diabetic control and management of diabetes.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88180664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.21767/2386-5180.100285
Jamuneswary Selvarajoo, Jasmine Kho Pei Ying, I. Tang
Extramedullary (extraosseous) plasmacytoma is a plasma cell tumor that grows within any soft tissue. It is a scarce neoplasm, characterized by monoclonal proliferation of plasma cells. Most of the lesions appear in the head and neck region, with the upper respiratory tract as the primary site. We here present a case of a young man who presented to us with similar presentation as of a patient with nasal polyps. Extra medullary plasmacytoma of the nasal cavity is relatively quite rare and should be in the back of our mind when we are dealing with unilateral nasal cavity masses.
{"title":"Nasal Extramedullary Plasmacytoma - A Rare Case","authors":"Jamuneswary Selvarajoo, Jasmine Kho Pei Ying, I. Tang","doi":"10.21767/2386-5180.100285","DOIUrl":"https://doi.org/10.21767/2386-5180.100285","url":null,"abstract":"Extramedullary (extraosseous) plasmacytoma is a plasma cell tumor that grows within any soft tissue. It is a scarce neoplasm, characterized by monoclonal proliferation of plasma cells. Most of the lesions appear in the head and neck region, with the upper respiratory tract as the primary site. We here present a case of a young man who presented to us with similar presentation as of a patient with nasal polyps. Extra medullary plasmacytoma of the nasal cavity is relatively quite rare and should be in the back of our mind when we are dealing with unilateral nasal cavity masses.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76288164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.21767/2386-5180.100281
I. Yi, Hao Li, M. Lim, Ernest Fu Weizhong, ini Cl Rao, Y. K. Chong, M. Bundele
Merkel Cell Carcinoma (MCC) is a rare, malignant neuroendocrine tumour belonging to the broad category of cancers called small round blue cell tumours. We present a case of this rare tumour occurring in a squamous papilloma of the external auditory canal of a patient, which regressed spontaneously and was subsequently excised with no evidence of recurrence.
{"title":"Merkel Cell Carcinoma Occurring Within A Squamous Papilloma of the External Ear Canal - A Case of Spontaneous Regression","authors":"I. Yi, Hao Li, M. Lim, Ernest Fu Weizhong, ini Cl Rao, Y. K. Chong, M. Bundele","doi":"10.21767/2386-5180.100281","DOIUrl":"https://doi.org/10.21767/2386-5180.100281","url":null,"abstract":"Merkel Cell Carcinoma (MCC) is a rare, malignant neuroendocrine tumour belonging to the broad category of cancers called small round blue cell tumours. We present a case of this rare tumour occurring in a squamous papilloma of the external auditory canal of a patient, which regressed spontaneously and was subsequently excised with no evidence of recurrence.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86720913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.21767/2386-5180.100287
Ch Kantharao, K. Swarna, J. Leelakrishna, J. Anusha, B. Asha, B. Bhavani
Aim: The aim of the present study is to prepare and evaluate Diclofenac sodium orodispresible tablets by using two super disintegrates, such as croscarmellose sodium (CCS) and sodium starch glycolate (SSG) in different concentrations by using direct compression technique. Materials and methods: We prepared 8 formulations by using direct compression technique. For this preparation, we have used different excipients along with the Dicofenac sodium drug such as sodium starch glycolate, carboxy methyl cellulose, lactose, mannitol, croscarmellose sodium, micro crystalline cellulose, magnesium stearate and talc. To identify the drug, we have used two methods such as Ultraviolet Spectroscopy and Capillary tube method. Results: All the formulations are disintegrated within one minute indicating the suitability of the product. Among all the formulations, F1, F5 and F6 showed maximum drug released within 30 minutes compared to other formulations. F1 formulation contains SSG 10%, F5 formulation contains CCS 10% and F6 contains CCS 20%. And F6 formulation gave 100% drug release when compared to F5 formulation which gave 82.3% of drug release within 30 minutes.
{"title":"Diclofenac Orodispersible Tablets: Formulation and In Vitro Evaluation","authors":"Ch Kantharao, K. Swarna, J. Leelakrishna, J. Anusha, B. Asha, B. Bhavani","doi":"10.21767/2386-5180.100287","DOIUrl":"https://doi.org/10.21767/2386-5180.100287","url":null,"abstract":"Aim: The aim of the present study is to prepare and evaluate Diclofenac sodium orodispresible tablets by using two super disintegrates, such as croscarmellose sodium (CCS) and sodium starch glycolate (SSG) in different concentrations by using direct compression technique. Materials and methods: We prepared 8 formulations by using direct compression technique. For this preparation, we have used different excipients along with the Dicofenac sodium drug such as sodium starch glycolate, carboxy methyl cellulose, lactose, mannitol, croscarmellose sodium, micro crystalline cellulose, magnesium stearate and talc. To identify the drug, we have used two methods such as Ultraviolet Spectroscopy and Capillary tube method. Results: All the formulations are disintegrated within one minute indicating the suitability of the product. Among all the formulations, F1, F5 and F6 showed maximum drug released within 30 minutes compared to other formulations. F1 formulation contains SSG 10%, F5 formulation contains CCS 10% and F6 contains CCS 20%. And F6 formulation gave 100% drug release when compared to F5 formulation which gave 82.3% of drug release within 30 minutes.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88571629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.21767/2386-5180.100280
S. AnitaRao, G. Ravishankar, T. Muralidhar, C. Soonu, B. Nageshwarrao
Aim: To determine the antibacterial effectiveness of calcium hydroxide and chlorhexidine gutta-percha points against Enterococcus faecalis and Streptococcus mutans Materials and methods: Eighteen extracted single-rooted human teeth were selected. After decoronation, root canals were prepared by using k files (Mani, Inc, Japan) up to master apical file size of forty. Following autoclave sterilization of these specimens, root canals were incubated at 37°C with E. faecalis and S. mutans for 24 hrs. Specimens were tested by inserting calcium hydroxid(Coltene/WhaledentInc.Germany), chlorhexidine (Roekoactivpoint, Germany) and conventional gutta-percha points (Endomax Guttapercha Points, Dento One Inc) for 1 hr, 3 hrs and 6 hrs respectively. Dentin chips collected after incubation were inoculated into agar plates and following an overnight incubation the colonies grown on agar plates were counted and interpreted as colony forming units. Results were tested statistically by using Friedman test for intragroup comparison at various time periods. One way Anova for intergroup comparison at different time periods. Overall two way Anova for comparison between the groups and the bacteria. Results: When compared with conventional gutta percha points, calcium hydroxide and chlorhexidine gutta-percha points showed significantly lower colony forming units against E. faecalis and S. mutans Conclusion: Chlorhexidine gutta percha points were more effective against E. faecalis and S. mutans than calcium hydroxide and conventional gutta-percha points.
目的:观察氢氧化钙和洗己定胶过胶穴位对粪肠球菌和变形链球菌的抑菌效果。材料和方法:选择18颗拔除的人单根牙齿。根管装饰完毕后，使用k锉(Mani, Inc, Japan)准备根管，根管尖锉大小为40。在对这些标本进行高压灭菌后，将根管与粪肠杆菌和变形链球菌一起在37°C下孵育24小时。分别用氢氧化钙(Coltene/WhaledentInc.Germany)、氯己定(Roekoactivpoint, Germany)和常规杜胶针(Endomax Guttapercha points, Dento One Inc.)浸泡1小时、3小时和6小时。将培养后收集的牙本质芯片接种到琼脂板上，经过一夜的培养，对琼脂板上生长的菌落进行计数并解释为菌落形成单位。采用Friedman检验对各时间段组内比较结果进行统计学检验。单因素方差分析用于不同时期的组间比较。两组和细菌之间的总体双向方差分析比较。结果:氢氧化钙和氯己定杜胶穴位对粪肠杆菌和变形链球菌的集落形成单位明显低于常规杜胶穴位。结论:氯己定杜胶穴位对粪肠杆菌和变形链球菌的防治效果优于氢氧化钙和常规杜胶穴位。
{"title":"Comparative Evaluation of Antimicrobial Efficacy of Calcium Hydroxide and Chlorhexidine Gutta Percha Points Against E. faecalis and S. mutans - An In Vitro Study","authors":"S. AnitaRao, G. Ravishankar, T. Muralidhar, C. Soonu, B. Nageshwarrao","doi":"10.21767/2386-5180.100280","DOIUrl":"https://doi.org/10.21767/2386-5180.100280","url":null,"abstract":"Aim: To determine the antibacterial effectiveness of calcium hydroxide and chlorhexidine gutta-percha points against Enterococcus faecalis and Streptococcus mutans Materials and methods: Eighteen extracted single-rooted human teeth were selected. After decoronation, root canals were prepared by using k files (Mani, Inc, Japan) up to master apical file size of forty. Following autoclave sterilization of these specimens, root canals were incubated at 37°C with E. faecalis and S. mutans for 24 hrs. Specimens were tested by inserting calcium hydroxid(Coltene/WhaledentInc.Germany), chlorhexidine (Roekoactivpoint, Germany) and conventional gutta-percha points (Endomax Guttapercha Points, Dento One Inc) for 1 hr, 3 hrs and 6 hrs respectively. Dentin chips collected after incubation were inoculated into agar plates and following an overnight incubation the colonies grown on agar plates were counted and interpreted as colony forming units. Results were tested statistically by using Friedman test for intragroup comparison at various time periods. One way Anova for intergroup comparison at different time periods. Overall two way Anova for comparison between the groups and the bacteria. Results: When compared with conventional gutta percha points, calcium hydroxide and chlorhexidine gutta-percha points showed significantly lower colony forming units against E. faecalis and S. mutans Conclusion: Chlorhexidine gutta percha points were more effective against E. faecalis and S. mutans than calcium hydroxide and conventional gutta-percha points.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91183232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.21767/2386-5180.100291
Shun Li, Xiang Wu, Zhong-Wei Ji, Bin Ru, Quan Wan, Wenbo Diao, Y. Niu
Aim: To explore the long-term outcome of patients with lumbar disc herniation (LDH) performed with percutaneous transforaminal endoscopic discectomy technique (PTED). Materials and methods: In this retrospective study, we collected the medical records from 974 patients with LDH who received PTED operation from May 2010 to July 2015. Follow-up measurements were performed at 1, 3, 6 and 12 months after surgery. Before and after surgery, visual analogue scale (VAS) was used for evaluating pain in leg and low back. Oswestry Disability Index (ODI) was used for evaluating the recovery of function. Japanese Orthopaedic Association (JOA) and modified MacNab criteria were used for clinical efficacy evaluation. Results: We found that the mean value of VAS and ODI were significantly decreased at each follow-up time points compared with that before operation (each p<0.01). The JOA showed significantly improving after the surgery through the follow-up period (each p<0.05). Furthermore, according to the modified MacNab criteria, the overall response of clinical efficiency was excellent in 32.7% patients and good in 54.9% patients.. Conclusion: PTED performed with broad, easy, and immediate surgery (BEIS) technique is an effective endoscopic discectomy approach for the treatment of LDH.
{"title":"Percutaneous Transforaminal Endoscopic Discectomy for Lumbar Disc Herniation: A Retrospective Study","authors":"Shun Li, Xiang Wu, Zhong-Wei Ji, Bin Ru, Quan Wan, Wenbo Diao, Y. Niu","doi":"10.21767/2386-5180.100291","DOIUrl":"https://doi.org/10.21767/2386-5180.100291","url":null,"abstract":"Aim: To explore the long-term outcome of patients with lumbar disc herniation (LDH) performed with percutaneous transforaminal endoscopic discectomy technique (PTED). Materials and methods: In this retrospective study, we collected the medical records from 974 patients with LDH who received PTED operation from May 2010 to July 2015. Follow-up measurements were performed at 1, 3, 6 and 12 months after surgery. Before and after surgery, visual analogue scale (VAS) was used for evaluating pain in leg and low back. Oswestry Disability Index (ODI) was used for evaluating the recovery of function. Japanese Orthopaedic Association (JOA) and modified MacNab criteria were used for clinical efficacy evaluation. Results: We found that the mean value of VAS and ODI were significantly decreased at each follow-up time points compared with that before operation (each p<0.01). The JOA showed significantly improving after the surgery through the follow-up period (each p<0.05). Furthermore, according to the modified MacNab criteria, the overall response of clinical efficiency was excellent in 32.7% patients and good in 54.9% patients.. Conclusion: PTED performed with broad, easy, and immediate surgery (BEIS) technique is an effective endoscopic discectomy approach for the treatment of LDH.","PeriodicalId":8195,"journal":{"name":"Annals of Clinical and Laboratory Research","volume":"128 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91373240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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