Raviraj Pansuriya, James Doutch, Bhagyesh Parmar, Suresh Kumar Kailasa, Najet Mahmoudi, Clare Hoskins and Naved I. Malek
The non-invasive nature and potential for sustained release make transdermal drug administration an appealing treatment option for cancer therapy. However, the strong barrier of the stratum corneum (SC) poses a challenge for the penetration of hydrophilic chemotherapy drugs such as 5-fluorouracil (5-FU). Due to its biocompatibility and capacity to increase drug solubility and permeability, especially when paired with chemical enhancers, such as oleic acid (OA), which is used in this work, choline glycinate ([Cho][Gly]) has emerged as a potential substance for transdermal drug delivery. In this work, we examined the possibility of transdermal delivery of 5-FU for the treatment of breast cancer using an ionic hydrogel formulation consisting of [Cho][Gly] with OA. Small angle neutron scattering, rheological analysis, field emission scanning electron microscopy, and dynamic light scattering analysis were used to characterize the ionic hydrogel. The non-covalent interactions present between [Cho][Gly] and OA were investigated by computational simulations and FTIR spectroscopy methods. When subjected to in vitro drug permeation using goat skin in a Franz diffusion cell, the hydrogel demonstrated sustained release of 5-FU and effective permeability in the order: [Cho][Gly]–OA gel > [Cho][Gly] > PBS (control). The hydrogel also demonstrated 92% cell viability after 48 hours for the human keratinocyte cell line (HaCaT cells) as well as the normal human cell line L-132. The breast cancer cell line MCF-7 and the cervical cancer cell line HeLa were used to study in vitro cytotoxicity that was considerably affected by the 5-FU-loaded hydrogel. These results indicate the potential of the hydrogel as a transdermal drug delivery vehicle for the treatment of breast cancer.
透皮给药具有非侵入性和持续释放的潜力,是治疗癌症的一种有吸引力的治疗方法。然而,角质层(SC)的强大屏障给亲水性化疗药物(如 5-氟尿嘧啶(5-FU))的渗透带来了挑战。由于胆碱甘氨酸([Cho][Gly])具有生物相容性和增加药物溶解度和渗透性的能力,特别是与本研究中使用的油酸(OA)等化学增强剂搭配使用时,胆碱甘氨酸已成为一种潜在的透皮给药物质。在这项工作中,我们研究了使用由 [Cho][Gly] 和 OA 组成的离子水凝胶配方透皮给药 5-FU 治疗乳腺癌的可能性。我们利用小角中子散射、流变学分析、场发射扫描电子显微镜和动态光散射分析对离子水凝胶进行了表征。计算模拟和傅立叶变换红外光谱法研究了[Cho][Gly]与 OA 之间的非共价相互作用。在弗朗兹扩散池中使用山羊皮进行体外药物渗透试验时,水凝胶显示出 5-FU 的持续释放和有效渗透性,其顺序为[Cho][Gly]-OA凝胶 > [Cho][Gly] > PBS(对照组)。48 小时后,水凝胶还显示人类角质细胞系(HaCaT 细胞)和正常人细胞系 L-132 的细胞存活率为 92%。乳腺癌细胞株 MCF-7 和宫颈癌细胞株 HeLa 被用来研究体外细胞毒性,5-FU 负载水凝胶对细胞毒性的影响很大。这些结果表明了水凝胶作为透皮给药载体治疗乳腺癌的潜力。
{"title":"A bio-ionic liquid based self-healable and adhesive ionic hydrogel for the on-demand transdermal delivery of a chemotherapeutic drug†","authors":"Raviraj Pansuriya, James Doutch, Bhagyesh Parmar, Suresh Kumar Kailasa, Najet Mahmoudi, Clare Hoskins and Naved I. Malek","doi":"10.1039/D4TB00510D","DOIUrl":"10.1039/D4TB00510D","url":null,"abstract":"<p >The non-invasive nature and potential for sustained release make transdermal drug administration an appealing treatment option for cancer therapy. However, the strong barrier of the stratum corneum (SC) poses a challenge for the penetration of hydrophilic chemotherapy drugs such as 5-fluorouracil (5-FU). Due to its biocompatibility and capacity to increase drug solubility and permeability, especially when paired with chemical enhancers, such as oleic acid (OA), which is used in this work, choline glycinate ([Cho][Gly]) has emerged as a potential substance for transdermal drug delivery. In this work, we examined the possibility of transdermal delivery of 5-FU for the treatment of breast cancer using an ionic hydrogel formulation consisting of [Cho][Gly] with OA. Small angle neutron scattering, rheological analysis, field emission scanning electron microscopy, and dynamic light scattering analysis were used to characterize the ionic hydrogel. The non-covalent interactions present between [Cho][Gly] and OA were investigated by computational simulations and FTIR spectroscopy methods. When subjected to <em>in vitro</em> drug permeation using goat skin in a Franz diffusion cell, the hydrogel demonstrated sustained release of 5-FU and effective permeability in the order: [Cho][Gly]–OA gel > [Cho][Gly] > PBS (control). The hydrogel also demonstrated 92% cell viability after 48 hours for the human keratinocyte cell line (HaCaT cells) as well as the normal human cell line L-132. The breast cancer cell line MCF-7 and the cervical cancer cell line HeLa were used to study <em>in vitro</em> cytotoxicity that was considerably affected by the 5-FU-loaded hydrogel. These results indicate the potential of the hydrogel as a transdermal drug delivery vehicle for the treatment of breast cancer.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":7.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140922679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tao Zhang, Liya Bai, Ran You, Meng Yang, Qian Chen, Yuanyuan Cheng, Zhanyin Qian, Yinsong Wang and Yuanyuan Liu
Melittin (Mel) is considered a promising candidate drug for the treatment of triple negative breast cancer (TNBC) due to its various antitumor effects. However, its clinical application is hampered by notable limitations, including hemolytic activity, rapid clearance, and a lack of tumor selectivity. Here, we designed novel biomimetic nanoparticles based on homologous tumor cell membranes and poly(lactic-co-glycolic acid) (PLGA)/poly(beta-aminoester) (PBAE), denoted MDM@TPP, which efficiently coloaded the cytolytic peptide Mel and the photosensitizer mTHPC. Both in vitro and in vivo, the MDM@TPP nanoparticles effectively mitigated the acute toxicity of melittin and exhibited strong TNBC targeting ability due to the homologous targeting effect of the tumor cell membrane. Under laser irradiation, the MDM@TPP nanoparticles showed excellent photodynamic performance and thus accelerated the release of Mel by disrupting cell membrane integrity. Moreover, Mel combined with photodynamic therapy (PDT) can synergistically kill tumor cells and induce significant immunogenic cell death, thereby stimulating the maturation of dendritic cells (DCs). In 4T1 tumor-bearing mice, MDM@TPP nanoparticles effectively inhibited the growth and metastasis of primary tumors and finally prevented tumor recurrence by improving the immune response.
{"title":"Homologous-targeting biomimetic nanoparticles co-loaded with melittin and a photosensitizer for the combination therapy of triple negative breast cancer†","authors":"Tao Zhang, Liya Bai, Ran You, Meng Yang, Qian Chen, Yuanyuan Cheng, Zhanyin Qian, Yinsong Wang and Yuanyuan Liu","doi":"10.1039/D3TB02919K","DOIUrl":"10.1039/D3TB02919K","url":null,"abstract":"<p >Melittin (Mel) is considered a promising candidate drug for the treatment of triple negative breast cancer (TNBC) due to its various antitumor effects. However, its clinical application is hampered by notable limitations, including hemolytic activity, rapid clearance, and a lack of tumor selectivity. Here, we designed novel biomimetic nanoparticles based on homologous tumor cell membranes and poly(lactic-<em>co</em>-glycolic acid) (PLGA)/poly(beta-aminoester) (PBAE), denoted MDM@TPP, which efficiently coloaded the cytolytic peptide Mel and the photosensitizer mTHPC. Both <em>in vitro</em> and <em>in vivo</em>, the MDM@TPP nanoparticles effectively mitigated the acute toxicity of melittin and exhibited strong TNBC targeting ability due to the homologous targeting effect of the tumor cell membrane. Under laser irradiation, the MDM@TPP nanoparticles showed excellent photodynamic performance and thus accelerated the release of Mel by disrupting cell membrane integrity. Moreover, Mel combined with photodynamic therapy (PDT) can synergistically kill tumor cells and induce significant immunogenic cell death, thereby stimulating the maturation of dendritic cells (DCs). In 4T1 tumor-bearing mice, MDM@TPP nanoparticles effectively inhibited the growth and metastasis of primary tumors and finally prevented tumor recurrence by improving the immune response.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":7.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Neera Singh, K. P. Srikanth, Vasanth Gopal, Monika Rajput, Geetha Manivasagam, K. G. Prashanth, Kaushik Chatterjee and Satyam Suwas
This work aimed to manufacture Ti–28.5Nb and Ti–40.0Nb (wt%) alloys in situ via selective laser melting (SLM) from Ti and Nb elemental powders. X-ray diffraction analysis revealed complete β-phase (cubic) in Ti–40.0Nb and a mixture of (α′′ orthorhombic + β cubic) phases in Ti–28.5Nb were formed, whereas few of the Nb particles remained only partially fused during manufacturing. The fraction of partially melted Nb particles was determined as ∼2 and ∼18% in Ti–28.5Nb and Ti–40Nb, respectively. Mechanical characterization revealed higher hardness and more strength in Ti–28.5Nb than in Ti–40.0Nb due to the presence of the α′′ phase in the former. Tribocorrosion tests reveal a significantly better wear-corrosion resistance for Ti–40.0Nb, as determined from a lower total volume loss in Ti–40.0Nb (∼2 × 10−4 mm−3) than in Ti–28.5Nb (∼13 × 10−2 mm−3). The lower volume loss and better corrosion resistance behavior are attributed to the β phase, which was dominant in Ti–40.0Nb. Cell studies reveal no toxicity for up to 7 days. Both the alloys were better at supporting cell proliferation than wrought Ti6Al4V. This study presents a route to preparing Ti–Nb alloys in situ by SLM that are promising candidates for biomedical applications.
{"title":"In situ production of low-modulus Ti–Nb alloys by selective laser melting and their functional assessment toward orthopedic applications","authors":"Neera Singh, K. P. Srikanth, Vasanth Gopal, Monika Rajput, Geetha Manivasagam, K. G. Prashanth, Kaushik Chatterjee and Satyam Suwas","doi":"10.1039/D4TB00379A","DOIUrl":"10.1039/D4TB00379A","url":null,"abstract":"<p >This work aimed to manufacture Ti–28.5Nb and Ti–40.0Nb (wt%) alloys <em>in situ via</em> selective laser melting (SLM) from Ti and Nb elemental powders. X-ray diffraction analysis revealed complete β-phase (cubic) in Ti–40.0Nb and a mixture of (α′′ orthorhombic + β cubic) phases in Ti–28.5Nb were formed, whereas few of the Nb particles remained only partially fused during manufacturing. The fraction of partially melted Nb particles was determined as ∼2 and ∼18% in Ti–28.5Nb and Ti–40Nb, respectively. Mechanical characterization revealed higher hardness and more strength in Ti–28.5Nb than in Ti–40.0Nb due to the presence of the α′′ phase in the former. Tribocorrosion tests reveal a significantly better wear-corrosion resistance for Ti–40.0Nb, as determined from a lower total volume loss in Ti–40.0Nb (∼2 × 10<small><sup>−4</sup></small> mm<small><sup>−3</sup></small>) than in Ti–28.5Nb (∼13 × 10<small><sup>−2</sup></small> mm<small><sup>−3</sup></small>). The lower volume loss and better corrosion resistance behavior are attributed to the β phase, which was dominant in Ti–40.0Nb. Cell studies reveal no toxicity for up to 7 days. Both the alloys were better at supporting cell proliferation than wrought Ti6Al4V. This study presents a route to preparing Ti–Nb alloys <em>in situ</em> by SLM that are promising candidates for biomedical applications.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":7.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141163174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ishfaq Ahmad Rather, Manzoor Ahmad, Pinaki Talukdar and Rashid Ali
Therapeutic applications have sparked increased interest in the use of synthetic anion receptors for ion transport across lipid membranes. In this context, the construction of synthetic transmembrane transporters for the physiologically important chloride ion is currently of enormous interest. As a result, considerable effort is being devoted to the design and synthesis of artificial transmembrane chloride ion transporters. However, only inadequate progress has been made in developing macrocyclic chloride ion transporters using the fundamental principles of supramolecular chemistry, and hence this field entails fostering investigations. In this investigation, the synthesis of two new double walled trifluorophenyl/phthalimide extended calix[4]pyrrole (C4P) receptors (3 and 7) has been successfully reported. 1H-NMR titration and HRMS studies confirmed the 1 : 1 binding stoichiometry of the chloride ion with these receptors in the solution phase (only receptor 3b was studied by 1H-NMR). Regarding ion transport of 3b and 7, when studied in the HPTS-based vesicular system, 3b showed better activity with an EC50 value of 0.39 μM. The detailed ion transport studies on 3b have revealed that ion transport occurs through the Cl−/NO3− antiport mode.
{"title":"Probing and evaluating transmembrane chloride ion transport in double walled trifluorophenyl/phthalimide extended calix[4]pyrrole-based supramolecular receptors†","authors":"Ishfaq Ahmad Rather, Manzoor Ahmad, Pinaki Talukdar and Rashid Ali","doi":"10.1039/D3TB02880A","DOIUrl":"10.1039/D3TB02880A","url":null,"abstract":"<p >Therapeutic applications have sparked increased interest in the use of synthetic anion receptors for ion transport across lipid membranes. In this context, the construction of synthetic transmembrane transporters for the physiologically important chloride ion is currently of enormous interest. As a result, considerable effort is being devoted to the design and synthesis of artificial transmembrane chloride ion transporters. However, only inadequate progress has been made in developing macrocyclic chloride ion transporters using the fundamental principles of supramolecular chemistry, and hence this field entails fostering investigations. In this investigation, the synthesis of two new double walled trifluorophenyl/phthalimide extended calix[4]pyrrole (C4P) receptors (<strong>3</strong> and <strong>7</strong>) has been successfully reported. <small><sup>1</sup></small>H-NMR titration and HRMS studies confirmed the 1 : 1 binding stoichiometry of the chloride ion with these receptors in the solution phase (only receptor <strong>3b</strong> was studied by <small><sup>1</sup></small>H-NMR). Regarding ion transport of <strong>3b</strong> and <strong>7</strong>, when studied in the HPTS-based vesicular system, <strong>3b</strong> showed better activity with an EC<small><sub>50</sub></small> value of 0.39 μM. The detailed ion transport studies on <strong>3b</strong> have revealed that ion transport occurs through the Cl<small><sup>−</sup></small>/NO<small><sub>3</sub></small><small><sup>−</sup></small> antiport mode.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":7.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141159351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ali Yadegari, Mahsa Akbarzadeh, Farshad Kargaran, Ramin Mirzaee, Iman Salahshoori, Marcos A. L. Nobre and Hossein Ali Khonakdar
The advent of polymer-based dielectrics marked a significant breakthrough in dielectric materials. However, despite their many advantages, they pose serious environmental threats. Therefore, in recent years, there has been growing interest in bio-based polymers as a sustainable alternative to traditional petroleum-based polymers. Their renewable nature and reduced environmental impact can fulfil the rising demand for eco-friendly substitutes. Beyond their ecological benefits, bio-based polymers also possess distinctive electrical properties that make them extremely attractive in a variety of applications. Considering these, herein, we present recent advancements in bio-based dielectric polymers and nanocomposites. First, the fundamental concepts of dielectric and polymer-based dielectric materials are covered. Then, we will delve into the discussion of recent advancements in the dielectric properties and thermal stability of bio-based polymers, including polylactic acid, polyhydroxyalkanoates, polybutylene succinate, starch, cellulose, chitosan, chitins, and alginates, and their nanocomposites. Other novel bio-based dielectric polymers and their distinct dielectric characteristics have also been pointed out. In an additional section, the piezoelectric properties of these polymers and their recent biomedical applications have been highlighted and discussed thoroughly. In conclusion, this paper thoroughly discusses the recent advances in bio-based dielectric polymers and their potential to revolutionize the biomedical industry while cultivating a more sustainable and greener future.
{"title":"Recent advancements in bio-based dielectric and piezoelectric polymers and their biomedical applications","authors":"Ali Yadegari, Mahsa Akbarzadeh, Farshad Kargaran, Ramin Mirzaee, Iman Salahshoori, Marcos A. L. Nobre and Hossein Ali Khonakdar","doi":"10.1039/D4TB00231H","DOIUrl":"10.1039/D4TB00231H","url":null,"abstract":"<p >The advent of polymer-based dielectrics marked a significant breakthrough in dielectric materials. However, despite their many advantages, they pose serious environmental threats. Therefore, in recent years, there has been growing interest in bio-based polymers as a sustainable alternative to traditional petroleum-based polymers. Their renewable nature and reduced environmental impact can fulfil the rising demand for eco-friendly substitutes. Beyond their ecological benefits, bio-based polymers also possess distinctive electrical properties that make them extremely attractive in a variety of applications. Considering these, herein, we present recent advancements in bio-based dielectric polymers and nanocomposites. First, the fundamental concepts of dielectric and polymer-based dielectric materials are covered. Then, we will delve into the discussion of recent advancements in the dielectric properties and thermal stability of bio-based polymers, including polylactic acid, polyhydroxyalkanoates, polybutylene succinate, starch, cellulose, chitosan, chitins, and alginates, and their nanocomposites. Other novel bio-based dielectric polymers and their distinct dielectric characteristics have also been pointed out. In an additional section, the piezoelectric properties of these polymers and their recent biomedical applications have been highlighted and discussed thoroughly. In conclusion, this paper thoroughly discusses the recent advances in bio-based dielectric polymers and their potential to revolutionize the biomedical industry while cultivating a more sustainable and greener future.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":7.0,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140913255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vaishnavi Dhawan, Paige Nicole Martin, Xiaoming Hu and Xinyan Tracy Cui
Invasive neural implants allow for high-resolution bidirectional communication with the nervous tissue and have demonstrated the ability to record neural activity, stimulate neurons, and sense neurochemical species with high spatial selectivity and resolution. However, upon implantation, they are exposed to a foreign body response which can disrupt the seamless integration of the device with the native tissue and lead to deterioration in device functionality for chronic implantation. Modifying the device surface by incorporating bioactive coatings has been a promising approach to camouflage the device and improve integration while maintaining device performance. In this work, we explored the novel application of a chondroitin sulfate (CS) based hydrophilic coating, with anti-fouling and neurite-growth promoting properties for neural recording electrodes. CS-coated samples exhibited significantly reduced protein-fouling in vitro which was maintained for up to 4-weeks. Cell culture studies revealed a significant increase in neurite attachment and outgrowth and a significant decrease in microglia attachment and activation for the CS group as compared to the control. After 1-week of in vivo implantation in the mouse cortex, the coated probes demonstrated significantly lower biofouling as compared to uncoated controls. Like the in vitro results, increased neuronal population (neuronal nuclei and neurofilament) and decreased microglial activation were observed. To assess the coating's effect on the recording performance of silicon microelectrodes, we implanted coated and uncoated electrodes in the mouse striatum for 1 week and performed impedance and recording measurements. We observed significantly lower impedance in the coated group, likely due to the increased wettability of the coated surface. The peak-to-peak amplitude and the noise floor levels were both lower in the CS group compared to the controls, which led to a comparable signal-to-noise ratio between the two groups. The overall single unit yield (% channels recording a single unit) was 74% for the CS and 67% for the control group on day 1. Taken together, this study demonstrates the effectiveness of the polysaccharide-based coating in reducing biofouling and improving biocompatibility for neural electrode devices.
{"title":"Investigation of a chondroitin sulfate-based bioactive coating for neural interface applications†","authors":"Vaishnavi Dhawan, Paige Nicole Martin, Xiaoming Hu and Xinyan Tracy Cui","doi":"10.1039/D4TB00501E","DOIUrl":"10.1039/D4TB00501E","url":null,"abstract":"<p >Invasive neural implants allow for high-resolution bidirectional communication with the nervous tissue and have demonstrated the ability to record neural activity, stimulate neurons, and sense neurochemical species with high spatial selectivity and resolution. However, upon implantation, they are exposed to a foreign body response which can disrupt the seamless integration of the device with the native tissue and lead to deterioration in device functionality for chronic implantation. Modifying the device surface by incorporating bioactive coatings has been a promising approach to camouflage the device and improve integration while maintaining device performance. In this work, we explored the novel application of a chondroitin sulfate (CS) based hydrophilic coating, with anti-fouling and neurite-growth promoting properties for neural recording electrodes. CS-coated samples exhibited significantly reduced protein-fouling <em>in vitro</em> which was maintained for up to 4-weeks. Cell culture studies revealed a significant increase in neurite attachment and outgrowth and a significant decrease in microglia attachment and activation for the CS group as compared to the control. After 1-week of <em>in vivo</em> implantation in the mouse cortex, the coated probes demonstrated significantly lower biofouling as compared to uncoated controls. Like the <em>in vitro</em> results, increased neuronal population (neuronal nuclei and neurofilament) and decreased microglial activation were observed. To assess the coating's effect on the recording performance of silicon microelectrodes, we implanted coated and uncoated electrodes in the mouse striatum for 1 week and performed impedance and recording measurements. We observed significantly lower impedance in the coated group, likely due to the increased wettability of the coated surface. The peak-to-peak amplitude and the noise floor levels were both lower in the CS group compared to the controls, which led to a comparable signal-to-noise ratio between the two groups. The overall single unit yield (% channels recording a single unit) was 74% for the CS and 67% for the control group on day 1. Taken together, this study demonstrates the effectiveness of the polysaccharide-based coating in reducing biofouling and improving biocompatibility for neural electrode devices.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":7.0,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/tb/d4tb00501e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140923745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mingsan Miao, Liang Guo, Jinyan Xue, Yuzhen Jia, Zhenzhen Cui and Huaixia Yang
The development of a simple, rapid, and sensitive technology for the simultaneous detection of mycotoxins is of great significance in ensuring the safety of foods and drugs. Herein, a fluorescence aptasensor with high sensitivity and reproducibility for the simultaneous detection of aflatoxin B1 (AFB1) and ochratoxin A (OTA) was developed. In this sensing system, AFB1 and OTA aptamers were co-immobilized on the surface of magnetic beads (MBs) to form a Y-shaped structure through the principle of complementary base pairing, and were used as recognition probes to specifically capture the target. Activators regenerated by electron transfer for atom transfer radical polymerization (ARGET ATRP) was used as a signal amplification strategy to improve the sensitivity. The initiator modified at the end of an antibody initiates the ARGET ATRP reaction. Different fluorescence signals were designed to achieve the simultaneous detection of OTA and AFB1 with limits of 426.18 and 79.55 fg mL−1 for AFB1 and OTA, respectively. In addition, experiments were conducted on three types of samples, and the recoveries of the two mycotoxins ranged from 87.30% to 109.50%, with relative standard deviations ranging from 0.50% to 4.92% under reproducible conditions. The results suggest that the developed aptasensor is sufficient to meet the different regulatory requirements of the two mycotoxins in food and drug safety and shows great potential.
开发一种简单、快速、灵敏的同时检测霉菌毒素的技术对于确保食品和药品安全具有重要意义。本研究开发了一种具有高灵敏度和高重现性的荧光传感器,用于同时检测黄曲霉毒素 B1(AFB1)和赭曲霉毒素 A(OTA)。在该传感系统中,通过碱基互补配对原理,将 AFB1 和 OTA 合体共同固定在磁珠(MBs)表面,形成 Y 型结构,并以此作为识别探针,特异性地捕获目标物。原子转移自由基聚合的电子转移再生引发剂(ARGET ATRP)被用作信号放大策略,以提高灵敏度。抗体末端修饰的引发剂启动 ARGET ATRP 反应。设计了不同的荧光信号以实现对 OTA 和 AFB1 的同时检测,AFB1 和 OTA 的检测限分别为 426.18 和 79.55 fg mL-1。此外,还对三种样品进行了实验,在重现性良好的条件下,两种霉菌毒素的回收率为 87.30% 至 109.50%,相对标准偏差为 0.50% 至 4.92%。结果表明,所开发的适配传感器足以满足食品和药品安全领域对这两种霉菌毒素的不同监管要求,并显示出巨大的潜力。
{"title":"A controllable Y-shaped DNA structure assisted aptasensor for the simultaneous detection of AFB1 and OTA based on ARGET ATRP†","authors":"Mingsan Miao, Liang Guo, Jinyan Xue, Yuzhen Jia, Zhenzhen Cui and Huaixia Yang","doi":"10.1039/D4TB00383G","DOIUrl":"10.1039/D4TB00383G","url":null,"abstract":"<p >The development of a simple, rapid, and sensitive technology for the simultaneous detection of mycotoxins is of great significance in ensuring the safety of foods and drugs. Herein, a fluorescence aptasensor with high sensitivity and reproducibility for the simultaneous detection of aflatoxin B<small><sub>1</sub></small> (AFB<small><sub>1</sub></small>) and ochratoxin A (OTA) was developed. In this sensing system, AFB<small><sub>1</sub></small> and OTA aptamers were co-immobilized on the surface of magnetic beads (MBs) to form a Y-shaped structure through the principle of complementary base pairing, and were used as recognition probes to specifically capture the target. Activators regenerated by electron transfer for atom transfer radical polymerization (ARGET ATRP) was used as a signal amplification strategy to improve the sensitivity. The initiator modified at the end of an antibody initiates the ARGET ATRP reaction. Different fluorescence signals were designed to achieve the simultaneous detection of OTA and AFB<small><sub>1</sub></small> with limits of 426.18 and 79.55 fg mL<small><sup>−1</sup></small> for AFB<small><sub>1</sub></small> and OTA, respectively. In addition, experiments were conducted on three types of samples, and the recoveries of the two mycotoxins ranged from 87.30% to 109.50%, with relative standard deviations ranging from 0.50% to 4.92% under reproducible conditions. The results suggest that the developed aptasensor is sufficient to meet the different regulatory requirements of the two mycotoxins in food and drug safety and shows great potential.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":7.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141077491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yijing Yao, Yiwen Zheng, Mingtai Wu, Yihui Gao, Qian Yu, Mengyao Liu, Xiaoxiao Luo, Rui Wang and Lixin Jiang
Pancreatic cancer is an aggressive and highly fatal malignant tumor. Recent studies have shown that cancer stem cells (CSCs) play an important role in resisting current therapeutic modalities. Furthermore, CD133 is highly expressed in CSCs. High-intensity focused ultrasound (HIFU) is a promising non-invasive therapeutic strategy for unresectable pancreatic cancers. In our study, we synthesized targeted CD133 organosilane nanomicelles by encapsulating perfluorohexane (PFH). The CD133 antibody on the surface could specifically bind to CD133-positive pancreatic cancer cells and selectively concentrate in pancreatic cancer tumor tissues. PFH was introduced to improve the ablation effect of HIFU due to its liquid–gas phase transition properties. By combining with the dorsal skinfold window chamber model (DSWC) of pancreatic cancer in nude mice, multiphoton fluorescence microscopy was used to evaluate the targeting effect of nanomicelles on pancreatic cancer tumor tissue. These multifunctional nanomicelles synergistically affected HIFU treatment of pancreatic cancer, providing an integrated research platform for diagnosing and treating pancreatic cancer with HIFU.
{"title":"CD133-targeted multifunctional nanomicelles for dual-modality imaging and synergistic high-intensity focus ultrasound (HIFU) ablation on pancreatic cancer in nude mice†","authors":"Yijing Yao, Yiwen Zheng, Mingtai Wu, Yihui Gao, Qian Yu, Mengyao Liu, Xiaoxiao Luo, Rui Wang and Lixin Jiang","doi":"10.1039/D4TB00091A","DOIUrl":"10.1039/D4TB00091A","url":null,"abstract":"<p >Pancreatic cancer is an aggressive and highly fatal malignant tumor. Recent studies have shown that cancer stem cells (CSCs) play an important role in resisting current therapeutic modalities. Furthermore, CD133 is highly expressed in CSCs. High-intensity focused ultrasound (HIFU) is a promising non-invasive therapeutic strategy for unresectable pancreatic cancers. In our study, we synthesized targeted CD133 organosilane nanomicelles by encapsulating perfluorohexane (PFH). The CD133 antibody on the surface could specifically bind to CD133-positive pancreatic cancer cells and selectively concentrate in pancreatic cancer tumor tissues. PFH was introduced to improve the ablation effect of HIFU due to its liquid–gas phase transition properties. By combining with the dorsal skinfold window chamber model (DSWC) of pancreatic cancer in nude mice, multiphoton fluorescence microscopy was used to evaluate the targeting effect of nanomicelles on pancreatic cancer tumor tissue. These multifunctional nanomicelles synergistically affected HIFU treatment of pancreatic cancer, providing an integrated research platform for diagnosing and treating pancreatic cancer with HIFU.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":7.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141077494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xu Wei, Chang Wan, Xu Peng, Yihao Luo, Mengyue Hu, Can Cheng, Shaoxiong Feng and Xixun Yu
Biomaterials with dual functions of osteoimmunomodulation and bone repair are very promising in the field of orthopedic materials. For this purpose, we prepared copper-based carbon dots (CuCDs) and doped them into oxychondroitin sulfate/poly-acrylamide hydrogel (OPAM) to obtain a hybrid hydrogel (CuCDs/OPAM). We evaluated its osteoimmunomodulatory and bone repair properties in vitro and in vivo. The obtained CuCDs/OPAM exhibited good rBMSCs-cytocompatibility and anti-inflammatory properties in vitro. It also could effectively promote rBMSCs differentiation and the expression of osteogenic differentiation factors from rBMSCs under an inflammatory environment. Moreover, CuCDs/OPAM could induce macrophage phenotype switching (from M1-type macrophages to M2-type macrophages) in vivo, which is beneficial for anti-inflammatory action and presents good osteoimmunomodulation capability to induce a bone immune microenvironment to promote the differentiation of rBMSCs. In conclusion, CuCDs/OPAM hydrogel has dual functions of osteoimmunomodulatory and bone repair and is a promising bone filling and repair material.
{"title":"Copper-based carbon dots modified hydrogel with osteoimmunomodulatory and osteogenesis for bone regeneration†","authors":"Xu Wei, Chang Wan, Xu Peng, Yihao Luo, Mengyue Hu, Can Cheng, Shaoxiong Feng and Xixun Yu","doi":"10.1039/D4TB00526K","DOIUrl":"10.1039/D4TB00526K","url":null,"abstract":"<p >Biomaterials with dual functions of osteoimmunomodulation and bone repair are very promising in the field of orthopedic materials. For this purpose, we prepared copper-based carbon dots (CuCDs) and doped them into oxychondroitin sulfate/poly-acrylamide hydrogel (OPAM) to obtain a hybrid hydrogel (CuCDs/OPAM). We evaluated its osteoimmunomodulatory and bone repair properties <em>in vitro</em> and <em>in vivo</em>. The obtained CuCDs/OPAM exhibited good rBMSCs-cytocompatibility and anti-inflammatory properties <em>in vitro</em>. It also could effectively promote rBMSCs differentiation and the expression of osteogenic differentiation factors from rBMSCs under an inflammatory environment. Moreover, CuCDs/OPAM could induce macrophage phenotype switching (from M1-type macrophages to M2-type macrophages) <em>in vivo</em>, which is beneficial for anti-inflammatory action and presents good osteoimmunomodulation capability to induce a bone immune microenvironment to promote the differentiation of rBMSCs. In conclusion, CuCDs/OPAM hydrogel has dual functions of osteoimmunomodulatory and bone repair and is a promising bone filling and repair material.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":7.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiacheng Wang, Zhenglong Dou, Li Xia and Nan Huang
Magnesium alloy is currently regarded as the most favourable biodegradable metal; however, obstacles remain to be overcome in terms of managing its corrosion and ensuring its biocompatibility. In this study, a metal–organic complex comprising Ca ions incorporated in tannic acid (TA) was prepared and used to coat magnesium alloy by chemical conversion and dipping processes, followed by modification with stearic acid (SA). This metal–organic complex coating was demonstrated to be homogeneous and compact, and it significantly improved the electrochemical corrosion resistance and long-term degradation behaviour of the coated samples. Consequently, the well-controlled release of Mg and Ca ions, as well as the osteo-compatible TA and SA molecules, promoted the proliferation of osteoblast cells. This metal–organic complex coating offers a promising modifying strategy for magnesium-based orthopaedic implants.
镁合金目前被认为是最理想的可生物降解金属;然而,在控制其腐蚀性和确保其生物相容性方面仍有许多障碍需要克服。在这项研究中,通过化学转化和浸渍工艺,制备了由钙离子和单宁酸(TA)组成的金属有机复合物,并将其用于镁合金涂层,然后用硬脂酸(SA)进行改性。结果表明,这种金属有机复合物涂层均匀致密,能显著提高涂层样品的耐电化学腐蚀性能和长期降解性能。因此,镁离子和钙离子的良好释放以及与骨兼容的 TA 和 SA 分子促进了成骨细胞的增殖。这种金属有机复合物涂层为镁基骨科植入物提供了一种前景广阔的改性策略。
{"title":"Metal–organic complex coating for enhanced corrosion control and biocompatibility on biodegradable magnesium alloy for orthopaedic implants†","authors":"Jiacheng Wang, Zhenglong Dou, Li Xia and Nan Huang","doi":"10.1039/D4TB00347K","DOIUrl":"10.1039/D4TB00347K","url":null,"abstract":"<p >Magnesium alloy is currently regarded as the most favourable biodegradable metal; however, obstacles remain to be overcome in terms of managing its corrosion and ensuring its biocompatibility. In this study, a metal–organic complex comprising Ca ions incorporated in tannic acid (TA) was prepared and used to coat magnesium alloy by chemical conversion and dipping processes, followed by modification with stearic acid (SA). This metal–organic complex coating was demonstrated to be homogeneous and compact, and it significantly improved the electrochemical corrosion resistance and long-term degradation behaviour of the coated samples. Consequently, the well-controlled release of Mg and Ca ions, as well as the osteo-compatible TA and SA molecules, promoted the proliferation of osteoblast cells. This metal–organic complex coating offers a promising modifying strategy for magnesium-based orthopaedic implants.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":7.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140923874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}