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Coronaviruses in pregnant women in Saudi Arabia: A systematic comparative review of MERS-CoV and SARS-CoV-2 沙特阿拉伯孕妇中的冠状病毒:MERS-CoV和严重急性呼吸系统综合征冠状病毒2型的系统比较综述
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2021-05-01 DOI: 10.4103/2305-0500.316621
M. Barry, Magdoleen Barry
Objective: To compare the effects of coronaviruses, such as Middle East respiratory syndrome coronavirus (MERS-CoV) or coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), on pregnant women in Saudi Arabia, and to understand the disease dynamics of these coronaviruses so as to konw how to provide care and management of infected pregnant women and infants. Methods: We conducted a systematic review of all published papers on MERS-CoV and SARS-CoV-2 infections in pregnant women in Saudi Arabia to identify knowledge gaps. Inclusion and exclusion criteria were based on the PRISMA system. The search included all papers which were published between September 1, 2012 and November 29, 2020 on pregnant women with MERS-CoV and/ or COVID-19 in Saudi Arabia. Of 26 papers screened, five were included in the analysis. Results: A total of 11 pregnant women with MERS-CoV and four with COVID-19 were reported in Saudi Arabia in the medical literature during the review period. The mean ages of the women were 33 and 31 years old for MERS-CoV and COVID-19, respectively. Maternal and fetal mortality in cases of MERS-CoV were 35% and 30%, respectively, while no maternal or fetal mortalities were reported in COVID-19 cases. Conclusions: Very limited data has emerged from Saudi Arabia on pregnant women MERS-CoV and COVID-19. With such high mortality observed with MERS-CoV compared to COVID-19, there is a need for greater reporting of cases to truly grasp the extent of these infections in pregnant women in a country where both coronaviruses are circulating.
目的:比较中东呼吸综合征冠状病毒(MERS-CoV)或由严重急性呼吸综合征2型冠状病毒(SARS-CoV-2)引起的2019冠状病毒病(新冠肺炎)等冠状病毒对沙特阿拉伯孕妇的影响,了解这些冠状病毒的疾病动态,从而了解如何为受感染的孕妇和婴儿提供护理和管理。方法:我们对沙特阿拉伯孕妇感染MERS-CoV和严重急性呼吸系统综合征冠状病毒2型的所有已发表论文进行了系统综述,以确定知识差距。纳入和排除标准基于PRISMA系统。搜索包括2012年9月1日至2020年11月29日期间发表的关于沙特阿拉伯患有MERS-CoV和/或新冠肺炎孕妇的所有论文。在筛选的26篇论文中,有5篇被纳入分析。结果:在审查期间,沙特阿拉伯的医学文献中共报告了11名患有MERS-CoV的孕妇和4名患有新冠肺炎的孕妇。MERS-CoV和新冠肺炎患者的平均年龄分别为33岁和31岁。MERS-CoV病例中的孕产妇和胎儿死亡率分别为35%和30%,而新冠肺炎病例中没有孕产妇或胎儿死亡报告。结论:沙特阿拉伯关于孕妇MERS-CoV和新冠肺炎的数据非常有限。与新冠肺炎相比,MERS-CoV的死亡率如此之高,在一个两种冠状病毒都在传播的国家,需要更多的病例报告,以真正掌握孕妇感染的程度。
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引用次数: 2
Effect of flaxseed supplementation on metabolic state, endocrine profiles, body composition and reproductive performance of sows 添加亚麻籽对母猪代谢状态、内分泌、身体成分和繁殖性能的影响
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2021-05-01 DOI: 10.4103/2305-0500.316625
Sukhjinder Kaur, A. Singh, M. Honparkhe, Ajeet Kumar, Prahlad Singh, U. Singh
Objective: To assess the effect of flaxseed supplementation on metabolic profile, endocrine concentrations, non-esterified fatty acids (NEFA), body composition variables, and reproductive performance of sows. Methods: All the 21 crossbred Large White Yorkshire sows were considered in the study period starting at day 1 of current farrowing when the feeding of specific supplemental ration was started until the day of subsequent farrowing (days 150-155) and were equally allocated into three groups. Group 1 served as the control group and followed their normal feeding schedule. Group 2 and group 3, in addition to their normal feeding schedule, were supplemented with flaxseed at a rate of 0.5% and 1.0% of the dry matter, respectively. Blood samples were collected 15 days prior to farrowing, on the day of farrowing (day 0), at weekly intervals until day 28 of lactation and at monthly intervals during gestation to harvest the plasma. Plasma was used to assess the metabolic and endocrine status of sows. Body weight of each sow and individual birth weight of all piglets born were measured. Results: Flaxseed supplementation led to decrease in plasma cholesterol and triglyceride levels in the supplemented groups than in the control group (P<0.05). Plasma estradiol-17β level was higher in group 2 than that in group 1 and 3 on day 90 of the gestation period (P<0.05). The mean plasma level of insulin-like growth factor 1 was higher in group 3 than that in group 1 and 2 both in late lactation (day 28) as well as in early gestation (day 30) (P<0.05). Plasma NEFA and weight gain were greater in sows of group 2 and 3 compared to those fed with the normal control diets (P<0.05). The proportion of pregnant sows relative to sows bred was 100.0% in group 2 and 3 and 85.7% in the control group. Piglet mortality was lower in group 2 and 3 compared to group 1 (P>0.05). Conclusions: Flaxseed improves endocrine profiles, NEFA concentrations and body weight, resulting in better pregnancy rate and litter size.
目的:评估补充亚麻籽对母猪代谢状况、内分泌浓度、非酯化脂肪酸(NEFA)、身体成分变量和繁殖性能的影响。方法:从本次分娩第1天开始,当开始饲喂特定补充日粮直到随后分娩的那一天(第150至155天)时,将21头大白约克郡杂交母猪全部纳入研究期,并将其平均分为三组。第1组作为对照组,并遵循其正常喂养计划。第2组和第3组,除了正常的喂养计划外,还分别以干物质的0.5%和1.0%的比例补充亚麻籽。在分娩前15天、分娩当天(第0天)、哺乳期第28天之前每周采集血样,妊娠期间每月采集血样以采集血浆。血浆用于评估母猪的代谢和内分泌状况。测量每头母猪的体重和所有出生仔猪的个体出生体重。结果:与对照组相比,添加亚麻籽组的血浆胆固醇和甘油三酯水平降低(P0.05)。结论:亚麻籽改善了内分泌状况、NEFA浓度和体重,从而提高了妊娠率和产仔数。
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引用次数: 2
Adansonia digitata aqueous leaf extract ameliorates dexamethasone-induced testicular injury in male Wistar rats 洋地黄水叶提取物对地塞米松致雄性Wistar大鼠睾丸损伤的改善作用
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2021-05-01 DOI: 10.4103/2305-0500.316623
Joseph Dare, A. Ojewale, O. Olaniyan, John Adole, G. Okotie, C. Akintayo, A. Dare
Objective: To evaluate the effects of aqueous leaf extract of Adansonia (A.) digitata L on dexamethasone-induced testicular damage in male Wistar rats. Methods: Twenty adult male Wistar rats weighing 170-190 g were divided into four groups. Group I received 0.5 mL of phosphate buffer orally for 28 days and served as the normal control group; group II received 10 mg/kg of dexamethasone (a synthetic glucocorticoid) intraperitoneally for 7 days and 0.5 mL of phosphate buffer orally for 21 days, group III received 10 mg/kg of dexamethasone for 7 days and 800 mg/kg of A. digitata extract orally for 21 days; group IV received 10 mg/kg of dexamethasone for 7 days and 300 mg/kg of vitamin-E orally for 21 days. Dexamethasone was administered intra-peritoneally for 7 days and all administration lasted for 28 days. The rats were sacrificed by anesthesia with diethyl ether and the testes of each animal were harvested. The testis was homogenized in 0.25 M sucrose at 4 °C for biochemical and histological analyses. Results: Administration of dexamethasone significantly decreased body weight, glucose-6-phosphate dehydrogenase (G6PDH), lactate dehydrogenase (LDH), superoxide dismutase (SOD), and glutathione peroxidase (GPx) (P<0.05), and significantly increased malondialdehyde (MDA) activities (P<0.05). The degeneration in the population of spermatogonia and vacuolation and abnormal widening of the interstitial spaces were observed in the rats treated with dexamethasone. However, administration of A. digitata significantly increased SOD, GPx, G6PDH, and LDH levels, significantly decreased MDA activities and improved the histoarchitecture of the testis (P<0.05). Conclusions: A. digitata may have an ameliorative effect on dexamethasone-induced testicular damage in Wistar rats because of its anti-inflammatory and antioxidant properties.
目的:探讨花楸叶水提物对地塞米松致雄性Wistar大鼠睾丸损伤的影响。方法:体重170 ~ 190 g的成年雄性Wistar大鼠20只,随机分为4组。ⅰ组患者口服磷酸盐缓冲液0.5 mL,连续28 d,作为正常对照组;II组患者腹腔注射地塞米松(一种合成糖皮质激素)10 mg/kg,连续7天,口服磷酸盐缓冲液0.5 mL,连续21天;III组患者口服地塞米松10 mg/kg,连续7天,口服地塞米松提取物800 mg/kg,连续21天;IV组给予地塞米松10 mg/kg口服7 d,维生素e 300 mg/kg口服21 d。地塞米松腹腔给药7 d,全部给药28 d。用乙醚麻醉处死大鼠,取各组睾丸。睾丸用0.25 M蔗糖在4°C下匀浆,进行生化和组织学分析。结果:地塞米松显著降低体重、葡萄糖-6-磷酸脱氢酶(G6PDH)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)活性(P<0.05),显著提高丙二醛(MDA)活性(P<0.05)。地塞米松给药后大鼠精原细胞群变性、空泡形成、间质异常增宽。而给药后睾丸组织SOD、GPx、G6PDH、LDH水平显著升高(P<0.05), MDA活性显著降低(P<0.05),组织结构明显改善(P<0.05)。结论:地塞米松对Wistar大鼠睾丸损伤的影响可能与地塞米松抗炎、抗氧化作用有关。
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引用次数: 0
Effect of royal jelly on in vitro fertilization and early embryo development following nicotine treatment in adult female rats 蜂王浆对成年雌性大鼠尼古丁处理后体外受精和早期胚胎发育的影响
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2021-05-01 DOI: 10.4103/2305-0500.316624
Z. Khodabandeh, V. Nejati, A. Shalizar-Jalali, G. Najafi, F. Rahmani
Objective: To scrutinize the protective role of royal jelly as an antioxidant on nicotine-induced changes in malondialdehyde (MDA) level, p53 expression, in vitro fertilization (IVF) rate, and early embryo development in adult female rats. Methods: A total of 56 adult female Wistar rats were divided into 8 groups (n=7 in each group). Group 1 served as an untreated control group, group 2, 3 and 4 received nicotine at a dose of 0.50, 1.00 and 2.00 mg/kg respectively, group 5 received royal jelly at a dose of 100.00 mg/kg, and group 6, 7 and 8 received 0.50, 1.00 and 2.00 mg/kg nicotine, respectively, with 100.00 mg/kg body weight royal jelly. Nicotine and royal jelly were administered daily for 49 days in the experimental groups intra-peritoneally and orally, respectively. At the end of the experimental period, p53 expression, IVF rate and early embryo development as well as MDA concentration were measured. Results: The IVF rate, number of cumulus oocytes, two-cell embryos and blastocysts decreased in the nicotine-treated groups in a dose-dependent manner. In addition, p53 mRNA expression and MDA levels increased in the nicotine-treated groups. Royal jelly co-administration led to partial improvement in the aforementioned parameters. Conclusions: Royal jelly may have a repro-protective effect in nicotine-administered female rats in terms of its anti-oxidant and anti-apoptotic properties.
目的:探讨蜂王浆作为抗氧化剂对尼古丁诱导的成年雌性大鼠丙二醛(MDA)水平、p53表达、体外受精(IVF)率和早期胚胎发育的保护作用。方法:取成年雌性Wistar大鼠56只,随机分为8组,每组7只。第1组为未处理对照组,第2、3、4组分别给予0.50、1.00、2.00 mg/kg剂量的尼古丁,第5组给予100.00 mg/kg剂量的蜂王浆,第6、7、8组分别给予0.50、1.00、2.00 mg/kg剂量的尼古丁,蜂王浆为100.00 mg/kg体重。试验组每日给予尼古丁和蜂王浆腹腔注射和口服,连续49天。实验结束时,检测p53表达、体外受精率、早期胚胎发育及MDA浓度。结果:尼古丁处理组体外受精率、卵丘细胞数、双细胞胚胎数和囊胚数均呈剂量依赖性降低。此外,尼古丁处理组p53 mRNA表达和MDA水平升高。蜂王浆共同给药导致上述参数的部分改善。结论:蜂王浆具有抗氧化和抗细胞凋亡的作用,对尼古丁给药的雌性大鼠具有再生保护作用。
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引用次数: 2
Vitamin E supplementation may negatively affect preimplantation development and mitochondrial ultrastructure of vitrified murine embryos 补充维生素E可能对玻璃化小鼠胚胎的植入前发育和线粒体超微结构产生负面影响
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2021-05-01 DOI: 10.4103/2305-0500.316626
Mimi-Sophia Sarbandi, Nor-Ashikin Noor Khan, N. Rahman, Z. Eshak, Fathiah Abdullah, M. Malek, Aqila-Akmal Kamal
Objective: To observe the effects of vitamin E on post-vitrification preimplantation development, gross morphology as well as mitochondrial distribution and ultrastructure. Methods: Twenty-four female C57BL/6NTac mice, aged 12-16 weeks, were randomly divided into four groups. Group A did not receive any treatment and served as the control group. Group B was treated with corn oil stripped of tocopherols and served as the vehicle group. Group C was treated with 60 mg/kg body weight of tocotrienol-rich-fraction with corn oil stripped of tocopherols. Group D was treated with 60 mg/kg body weight of alpha-tocopherol with corn oil stripped of tocopherols. All treatments were administered orally for 7 consecutive days. After superovulation and mating with fertile males, 2-cell stage embryos were harvested for vitrification. Post vitrification development in vitro, gross morphology and ultrastructure were compared between groups. Results: The number of 2 and 8-cell embryo, and blastocysts in the treatment and control groups were not significantly different (P>0.05). Following vitrification, all 2-cell embryos had equal-sized blastomeres and intact zona pellucida. Mitochondrial aggregation toward the perinuclear region was seen in all of the treatment groups. Both groups C and D had vacuolated mitochondria, which was reflected in the trend of preimplantation development reduction. Conclusions: Vitamin E supplementation of 60 mg/kg body weight does not improve the viability of healthy embryos according to this study. As a result, the most effective dose of vitamin E supplementation may be determined by the initial quality of the embryos.
目的:观察维生素E对大鼠玻璃化后种植前发育、毛囊形态及线粒体分布和超微结构的影响。方法:24只12 ~ 16周龄雌性C57BL/6NTac小鼠随机分为4组。A组不进行任何治疗,作为对照组。B组以玉米油剥去生育酚处理,作为载体组。C组以60 mg/kg体重的富生育三烯馏分加玉米油去生育酚处理。D组以60 mg/kg体重的α -生育酚加玉米油剥去生育酚。所有治疗均口服,连续7天。在超排卵并与可育雄性交配后,收集2细胞期胚胎进行玻璃化。体外玻璃化发育后,比较各组的大体形态和超微结构。结果:治疗组和对照组2、8细胞胚数、囊胚数差异无统计学意义(P < 0.05)。玻璃化后,所有2细胞胚胎均有大小相等的卵裂球和完整的透明带。在所有治疗组中,线粒体向核周区域聚集。C组和D组均有空泡化线粒体,这反映在着床前发育减少的趋势上。结论:本研究表明,添加60 mg/kg体重的维生素E不能提高健康胚胎的存活率。因此,维生素E补充的最有效剂量可能取决于胚胎的初始质量。
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引用次数: 0
Excess iodine exposure: An emerging area of concern for male reproductive physiology in the post-salt iodization era 过量碘暴露:后盐加碘时代男性生殖生理学关注的一个新领域
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2021-05-01 DOI: 10.4103/2305-0500.316622
Arijit Chakraborty
To prevent iodine deficiency disorders, the universal salt iodization programme has been introduced all over the globe, including environmentally iodine sufficient regions irrespective of their iodine status. As a result, iodine-induced thyroid dysfunctions namely hyperthyroidism, hypothyroidism, autoimmune thyroid diseases, endemic goiter and even thyroid cancer including infertility, still births, abortions and embryo toxicity have emerged as a major public health problem. In other words, the consequence of iodine deficiency and excess is almost ‘U’-shaped. Hypothyroidism caused by iodine deficiency affects reproductive functions of organisms; however, such undesirable effects of iodine overload on male gonadal physiology together with hormonal profiles are yet to be adequately explored. The discovery of iodide transporter in the testis justifies an independent role of iodine in male reproductive function, which is not entirely known. Recent studies on human subjects and animal models are now revealing further perceptions into the effect of excess iodine on male infertility with euthyroid status. Excess iodine exposure has been linked with deterioration of structural and functional changes of testis leading to compromised spermatogenesis by affecting various cellular and molecular signaling pathways culminating into disrupted the blood-testis barrier and cytoskeleton. This review provides an update and summarizes various novel insights of excess iodine exposure on reproduction by establishing the independent role of iodine on male reproductive endocrinology, which might help in formulating future strategies to prevent iodine-induced male infertility, an emerging global concern, especially in the post-salt iodization era.
为了预防缺碘症,全球各地都推行了普遍食盐加碘方案,包括环境碘充足的地区,不论其碘状况如何。因此,碘引起的甲状腺功能障碍,即甲状腺机能亢进、甲状腺机能减退、自身免疫性甲状腺疾病、地方性甲状腺肿,甚至包括不孕症、死胎、流产和胚胎毒性在内的甲状腺癌,已成为一个重大的公共卫生问题。换句话说,碘缺乏和过量的后果几乎是U型的。缺碘引起的甲状腺功能减退影响生物体的生殖功能;然而,碘超载对男性性腺生理和激素谱的不良影响尚未得到充分探讨。睾丸中碘转运体的发现证明了碘在男性生殖功能中的独立作用,这一点尚不完全清楚。最近对人类受试者和动物模型的研究现在进一步揭示了过量碘对甲状腺功能正常的男性不育症的影响。过量的碘暴露与睾丸结构和功能变化的恶化有关,通过影响各种细胞和分子信号通路,最终破坏血睾丸屏障和细胞骨架,从而导致精子发生受损。本文通过建立碘对男性生殖内分泌的独立作用,提供了碘过量暴露对生殖的最新和总结的各种新见解,这可能有助于制定未来的策略来预防碘诱导的男性不育,这是一个新兴的全球关注的问题,特别是在后盐碘化时代。
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引用次数: 2
Combined effects of Gymnema sylvestre and Pergularia daemia on letrozole-induced polycystic ovarian syndrome in rats 来曲唑诱导大鼠多囊卵巢综合征的联合作用
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2021-03-01 DOI: 10.4103/2305-0500.311610
Sudhakar Pachiappan, Kothai Ramalingam, Arul Balasubramanian
Objective: To investigate the combined therapeutic potential of Gymnema (G.) sylvestre and Pergularia (P.) daemia on letrozole-induced polycystic ovarian syndrome (PCOS) in rats. Methods: Thirty six healthy female Wistar rats with regular estrus cycles were randomly divided into six groups each of 6. Group I received 1 mL of 0.5% carboxyl methyl cellulose orally and served as the vehicle control group, while groups II to VI were treated with letrozole (1 mg/kg body weight p. o.) for 21 days to induce PCOS. After induction of PCOS, group II served as the PCOS control group, without treatment; group III received metformin (20 mg/kg body weight p. o.) as the standard group, and groups IV to VI received G. sylvestre (100 mg/kg body weight p. o.), P. daemia (300 mg/kg body weight p. o.), and the combination of G. sylvestre and P. daemia, respectively, for 28 days. Vaginal smears were collected from all rats daily throughout the study to determine the phases of the estrus cycle. After completing the treatment schedule, oral glucose tolerance test, serum lipid profile and reproductive hormonal analysis were carried out. Subsequently, the rats were sacrificed to collect ovary and uterus for histopathological examination. Results: The PCOS control rats showed a significant irregularity in the estrus cycle, hyperglycemia, and the altered serum lipid profile such as the increased low and very low density lipoprotein, triglyceride, and decreased high density lipoproteins. In addition, the PCOS control rats showed a significant increase in serum luteinizing hormone, testosterone, and estrogen, and decrease in follicle stimulating hormone and progesterone. These changes were significantly revoked in all the treatment groups. The test drugs also significantly reduced the gained ovary weight (P<0.001), and histopathology of the ovary showed almost normal ovary. Among the treatment groups, the group of combination treatment of G. sylvestre and P. daemia showed superior ameliorative results in PCOS parameters. Conclusions: Combination of G. sylvestre and P. daemia presents potent synergistic activity against hyperandrogenism, hyperinsulinemia, anovulation and follicular cysts in letrozole-induced PCOS rats.
目的:探讨金合欢(Gymnema(G.)sylvestre)和Pergular ia(P.)daemia联合治疗来曲唑诱导的大鼠多囊卵巢综合征(PCOS)的疗效。方法:将36只发情周期正常的健康雌性Wistar大鼠随机分为6组,每组6只。第一组口服1mL 0.5%羧甲基纤维素并作为载体对照组,而第二至第六组用来曲唑(1mg/kg体重口服)治疗21天以诱导PCOS。PCOS诱导后,Ⅱ组为PCOS对照组,不治疗;第III组接受二甲双胍(20mg/kg体重p.o.)作为标准组,第IV至VI组分别接受G.sylvestre(100mg/kg体重p.o.o.)、p.daemia(300mg/kg体重p.o.o.o.)以及G.sylvestre和p.daemi的组合,持续28天。在整个研究过程中,每天从所有大鼠身上采集阴道涂片,以确定发情周期的阶段。在完成治疗计划后,进行口服糖耐量试验、血脂谱和生殖激素分析。随后,处死大鼠以收集卵巢和子宫进行组织病理学检查。结果:PCOS对照大鼠发情周期明显不规则,高血糖,血脂变化,如低密度和极低密度脂蛋白、甘油三酯升高,高密度脂蛋白降低。此外,多囊卵巢综合征对照大鼠血清黄体生成素、睾酮和雌激素显著增加,卵泡刺激素和孕酮显著减少。在所有治疗组中,这些变化均被显著撤销。试验药物还显著降低了增加的卵巢重量(P<0.001),卵巢的组织病理学显示卵巢几乎正常。在治疗组中,西尔维斯特和达氏疟原虫联合治疗组在PCOS参数方面显示出优越的改善效果。结论:在来曲唑诱导的PCOS大鼠中,sylvestre和P.daemia联合用药对高雄激素血症、高胰岛素血症、无排卵和卵泡囊肿具有强大的协同作用。
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引用次数: 3
Evaluation of pig oocyte in vitro maturation and fertilization using three gonadotropin-based hormonal compounds 以促性腺激素为基础的三种激素化合物对猪卵母细胞体外成熟和受精的评价
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2021-03-01 DOI: 10.4103/2305-0500.311618
Rosario Santiago-Rodriguez, Alma Álvarez-Guerrero, Fernando Garcia-Gonzalez, A. Alcántar-Rodríguez, A. Medrano
Objective: To evaluate the effect of human chorionic gonadotropins (hCG) and equine chorionic gonadotropins (eCG) on in vitro gilt oocyte maturation and embryonic development, using frozen semen for fertilization. Methods: Two independent experiments (6 replicates each) were carried out to evaluate gilt oocyte maturation, and fertilization and embryonic development by using ovaries from a local abattoir. Totally, 712 oocytes were randomly distributed in four-well dishes to receive Novormon (eCG 5.0 IU), PG600 (eCG 5.0 IU and hCG 2.5 IU), Chorulon (hCG 5.0 IU), or no hormones. Oocytes were incubated with 5% CO2, 95% air and saturation humidity at 39 °C for 44 h. Maturation of the oocytes to metaphase II was assessed by using the aceto-orcein technique. In addition, 741 oocytes were used and randomly distributed in four-well dishes, and then oocyte maturation was carried out as mentioned, but matured oocytes were washed and placed in fertilization medium with frozen-thawed sperm. Gametes were co-incubated for 7 h, and then washed and placed in development medium, and incubated for further 7 days, at which time embryonic development was evaluated. Fertilization and embryo development media were not supplemented with the studied hormones. Results: Novormon (eCG) and PG600 (eCG+hCG) treatments significantly improved the percentages of metaphase II oocytes compared to the control group (P<0.05). Furthermore, a significant increase was also observed in the young blastocyst stage between the control group and the PG600 treatment group (P<0.05). Conclusions: Hormonal products Novormon (eCG) and PG600 (eCG+hCG) can obtain the highest percentages of in vitro maturation in gilt oocytes; however, this effect is not transferred to fertilization rates.
目的:探讨人绒毛膜促性腺激素(hCG)和马绒毛膜促性腺激素(eCG)对体外后备母猪卵母细胞成熟和胚胎发育的影响。方法:采用当地某屠宰场的卵巢,进行2个独立实验(每个实验6个重复),对后备母猪卵母细胞成熟、受精和胚胎发育进行评价。将712个卵母细胞随机分布于四孔培养皿中,分别给予Novormon (eCG 5.0 IU)、PG600 (eCG 5.0 IU, hCG 2.5 IU)、Chorulon (hCG 5.0 IU)和无激素。卵母细胞在39°C、5% CO2、95%空气和饱和湿度条件下孵育44小时。卵母细胞成熟到中期II的情况采用aceto-orcein技术进行评估。另外,使用741个卵母细胞,随机分布在四孔培养皿中,然后按照上述方法进行卵母细胞成熟,但将成熟的卵母细胞洗涤后置于冷冻解冻精子受精培养基中。配子共孵育7 h,洗涤后置于发育培养基中,再孵育7 d,观察胚胎发育情况。受精和胚胎发育培养基中未添加所研究的激素。结果:与对照组相比,Novormon (eCG)和PG600 (eCG+hCG)治疗可显著提高中期II期卵母细胞百分比(P<0.05)。此外,PG600处理组与对照组相比,幼胚期也显著增加(P<0.05)。结论:激素产品Novormon (eCG)和PG600 (eCG+hCG)在镀金卵母细胞中体外成熟率最高;然而,这种影响不会转移到受精率上。
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引用次数: 1
Antifertility effects of 60-day oral gavage of ethanol extract of Spondias mombin leaves in guinea pigs: A biochemical, reproductive and histological study 灌胃海绵叶乙醇提取物60天对豚鼠的抗生育作用:生化、生殖和组织学研究
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2021-03-01 DOI: 10.4103/2305-0500.311609
O. Ogunro, M. Yakubu
Objective: To investigate the impact of ethanol extract of Spondias (S.) mombin leaves on the biochemical parameters of testicular function, hormonal profile, sperm profile and reproductive outcomes of guinea pigs. Methods: Sexually matured male [(1000.40±8.12) g] and female [(810.00±7.09) g] guinea pigs were used. In testicular function study, a total of 24 male guinea pigs were randomised into 4 groups of 6 guinea pigs each. Group A (control) were orally administered 1 mL of physiological saline, once daily for 60 days while groups B, C and D were treated like the control group except that they were orally administered 100, 250 and 500 mg/kg body weight of ethanol extract of S. mombin leaves. For the fertility study, the same animal groupings and treatments in the testicular function study were adopted. The male guinea pigs were paired with the females (1:1) and afterwards examined for pregnancy outcomes. Results: The ethanol extract of S. mombin leaves contained saponins, alkaloids, flavonoids, tannins, steroids, phenolics, phlobatannins, cardiac glycosides, cardenolides and dienolides with saponins (4.80 mg/mL) occurring the most whilst cardenolides and dienolides (0.08 mg/mL) were the least. The ethanol extract of S. mombin leaves significantly and dose dependently reduced the activities of alkaline phosphatase, glutamate dehydrogenase, 3-hydroxy-3-methylglutaryl coenzyme A reductase, malic enzyme, 17-β-hydroxysteroid dehydrogenase, lactate dehydrogenase, catalase and superoxide dismutase as well as levels of testosterone, glycogen, total protein and ascorbic acid in the testes when compared with the control group (P<0.05). All the doses of ethanol extract of S. mombin leaves also reduced the levels of sorbitol dehydrogenase, 3-β-hydroxysteroid dehydrogenase, glucose-6-phosphate dehydrogenase and sialic acid whereas the levels of testicular acid phosphatase, gamma glutamyl transferase and cholesterol increased dose dependently (P<0.05). The serum luteinising hormone, testosterone and estradiol were reduced after the administration of ethanol extract of S. mombin leaves whereas levels of serum follicle stimulating hormone increased significantly. The 100 and 200 mg/kg body weight of ethanol extract of S. mombin leaves increased the testosterone/estradiol ratios whilst the 500 mg/kg body weight of ethanol extract of S. mombin leaves decreased it. The sperm motility, sperm count, normal sperm morphology, sperm density, sperm viability and semen viscosity were significantly reduced in the ethanol extract of S. mombin leaves-treated guinea pigs (P<0.05) whereas the head-, tail- and neck-defects increased significantly when compared with the control group (P<0.05). In contrast, the semen volume and pH were not significantly altered by the ethanol extract of S. mombin leaves (P>0.05). The ethanol extract of S. mombin leaves at both 100 and 250 mg/kg body weight significantly reduced the total number, circumference, weight and length of the pups whe
目的:研究海棘叶乙醇提取物对豚鼠睾丸功能生化指标、激素谱、精子谱及生殖结局的影响。方法:选用性成熟雄性豚鼠[(1000.40±8.12)g]和雌性豚鼠[(810.00±7.09)g]。在睾丸功能研究中,24只雄性豚鼠随机分为4组,每组6只。A组(对照组)灌胃生理盐水1 mL,每日1次,连用60 D; B、C、D组除灌胃山楂叶乙醇提取物100、250、500 mg/kg体重外,与对照组处理相同。生殖研究采用与睾丸功能研究相同的动物分组和处理方法。雄性豚鼠与雌性(1:1)配对,随后检查妊娠结局。结果:山楂叶乙醇提取物中含有皂苷、生物碱、黄酮类、单宁、甾体、酚类、酚类、酚类、心苷类、桂皮内酯和二烯内酯,其中皂苷含量最多(4.80 mg/mL),桂皮内酯和二烯内酯含量最少(0.08 mg/mL)。与对照组相比,山楂叶乙醇提取物显著且剂量依赖地降低了睾丸中碱性磷酸酶、谷氨酸脱氢酶、3-羟基-3-甲基戊二酰辅酶A还原酶、苹果酸酶、17-β-羟基类固醇脱氢酶、乳酸脱氢酶、过氧化氢酶和超氧化物歧化酶的活性,以及睾丸激素、糖原、总蛋白和抗坏血酸水平(P0.05)。100和250 mg/kg体重的山楂叶乙醇提取物均显著降低了幼鼠的总数、周长、重量和长度,而500 mg/kg体重的山楂叶乙醇提取物处理的大鼠未产生幼鼠。250和500 mg/kg体重的山楂叶乙醇提取物可诱导精子小管变性和坏死,生发上皮内出现空泡,少量精子完全消失。其中,山楂叶乙醇提取物在500 mg/kg体重时对各项参数的影响最为显著。结论:山楂叶通过内分泌失调、抗生精活性、睾丸功能障碍和氧化应激诱导雄性豚鼠不育,这可能与山楂叶中皂苷、生物碱、黄酮类、单宁、类固醇、酚类物质和心脏苷的存在有关。
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引用次数: 5
Aphrodisiac potential of Polyalthia bullata (Tongkat Ali) in fowl 东革阿里对家禽的壮阳作用
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2021-03-01 DOI: 10.4103/2305-0500.311615
J. Vejayan, Yasmin Yahya, S. Chakravarthi, H. Ibrahim, Aida Yun
Objective: To study the aphrodisiac potential of Polyalthia (P.) bullata in fowl. Methods: In this study, testosterone, as an indicator of the aphrodisiac potential of P. bullata, was investigated for its release from TM3 Leydig cells grown in vitro and in 4 fowls given capsules containing P. bullata at a dose of 10 mg in each capsule twice a day, for 50 days. In the latter in vivo evaluation, mating behaviours were additionally determined after the treated fowls were released to the individual hens, and their testes and liver were dissected for histological examinations. Blood drawn from the fowls was assessed for any changes in diagnostic parameters. Results: In the in vitro test (TM3 Leydig cells), P. bullata was able to increase testosterone to 0.48 nmol/L within 72 h of incubation, compared to the untreated control with only 0.18 nmol/L, i.e., an increase of 170%. In the in vivo test, outcomes in the fowls dosed with P. bullata showed similar positive elevations of testosterone to (9.72±1.10) nmol/L in comparison to the controls that showed a level of only (4.05±0.84) nmol/L. Total frequencies of mating behaviours were observed (wing flapping, body shakes, crowing and beak pecking) to be 23 counts for the test compared to only 15 for the control fowls. Histological examination of the male reproductive organs provided evidence of testosterone boosting based on an observable increase in the activity at the seminiferous tubules of testis tissues without any damaging effects, compared to the controls. In the nine diagnostic blood parameters assessed, including alanine aminotransferase, aspartate aminotransferase, and gamma glutamyltransferase, none was remarkably elevated compared to the controls. The histological changes in the liver were not severe and mainly consisted of only localized moderate but recoverable obstructions and swellings of the vessels and tubules. Conclusions: P. bullata is able to boost testosterone both in vitro and in vivo, with no acute toxicities.
目的:研究大叶茯苓对家禽的壮阳作用。方法:通过体外培养的TM3间质细胞和4只鸡的体外培养,研究睾丸激素(睾丸激素)的释放情况,每个胶囊10 mg,每天2次,连续50天。在后者的体内评估中,将处理后的鸡放归母鸡个体后,进一步测定交配行为,并解剖其睾丸和肝脏进行组织学检查。从家禽身上抽血评估诊断参数是否有任何变化。结果:在体外实验(TM3间质细胞)中,在培养72h内,黄颡虾能将睾酮水平提高到0.48 nmol/L,而未处理的对照组仅为0.18 nmol/L,即提高了170%。在体内试验中,与对照组(4.05±0.84)nmol/L的睾酮水平相比,给药鸡的睾酮水平呈(9.72±1.10)nmol/L的阳性升高,对照组睾酮水平为(9.72±1.10)nmol/L。在试验中观察到交配行为的总频率(拍打翅膀、摇动身体、啼叫和啄嘴)为23次,而对照鸡只有15次。与对照组相比,男性生殖器官的组织学检查提供了睾丸激素增加的证据,这是基于睾丸组织精管活动的可观察到的增加,而没有任何破坏性影响。在评估的9个诊断血液参数中,包括丙氨酸转氨酶、天冬氨酸转氨酶和谷氨酰转氨酶,与对照组相比,没有一个明显升高。肝脏的组织学改变并不严重,主要是局部、中度但可恢复的血管和小管阻塞和肿胀。结论:在体内和体外均能提高睾酮水平,且无急性毒性。
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引用次数: 1
期刊
Asian Pacific Journal of Reproduction
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