Pub Date : 2022-05-01DOI: 10.4103/2305-0500.346092
M. Alomar
Objective: To investigate antioxidant potential of pentoxifylline on spermatozoa of small ruminants including rams and bucks. Methods: The levels of hydrogen peroxide (H2O2) production in ram and buck spermatozoa incubated with 0 (control), 4 and 8 mM of pentoxifylline were measured after 45-min incubation. Then, the motility parameters of ram and buck spermatozoa incubated with 0 (control), 1 mM of H2O2, 1 mM of H2O2 plus 4 mM of pentoxifylline, and 1 mM of H2O2 plus 8 mM of pentoxifylline were analysed. H2O2 was estimated using a fluorometric assay and spermatozoa motility characteristics were analyzed using computer aided sperm analyzer. Results: Pentoxifylline significantly decreased the levels of H2O2 produced from both ram and buck spermatozoa (P<0.05), and significant lower rates of H2O2 formation were noted when 8 mM of pentoxifylline was added (P<0.05). The values of all sperm motility parameters for the two species significantly decreased after incubation with H2O2 (P<0.05). In contrast, when the spermatozoa were incubated with both H2O2 and two concentrations of pentoxifylline, the motility values rose significantly compared to those incubated with H2O2 alone (P<0.05). For both ram and buck sperm samples, the rapid and static subpopulation motility parameters were the most affected categories by pentoxifylline addition compared to the medium and slow categories. Conclusions: Pentoxifylline possesses an antioxidant capacity proved by its ability of reducing H2O2 levels as well as by increasing motility values of stressed spermatozoa. Therefore, pentoxifylline could be recommended as antioxidant additive for spermatozoa of small ruminants under stress conditions.
{"title":"Antioxidant potential of pentoxifylline on spermatozoa of small ruminants","authors":"M. Alomar","doi":"10.4103/2305-0500.346092","DOIUrl":"https://doi.org/10.4103/2305-0500.346092","url":null,"abstract":"Objective: To investigate antioxidant potential of pentoxifylline on spermatozoa of small ruminants including rams and bucks. Methods: The levels of hydrogen peroxide (H2O2) production in ram and buck spermatozoa incubated with 0 (control), 4 and 8 mM of pentoxifylline were measured after 45-min incubation. Then, the motility parameters of ram and buck spermatozoa incubated with 0 (control), 1 mM of H2O2, 1 mM of H2O2 plus 4 mM of pentoxifylline, and 1 mM of H2O2 plus 8 mM of pentoxifylline were analysed. H2O2 was estimated using a fluorometric assay and spermatozoa motility characteristics were analyzed using computer aided sperm analyzer. Results: Pentoxifylline significantly decreased the levels of H2O2 produced from both ram and buck spermatozoa (P<0.05), and significant lower rates of H2O2 formation were noted when 8 mM of pentoxifylline was added (P<0.05). The values of all sperm motility parameters for the two species significantly decreased after incubation with H2O2 (P<0.05). In contrast, when the spermatozoa were incubated with both H2O2 and two concentrations of pentoxifylline, the motility values rose significantly compared to those incubated with H2O2 alone (P<0.05). For both ram and buck sperm samples, the rapid and static subpopulation motility parameters were the most affected categories by pentoxifylline addition compared to the medium and slow categories. Conclusions: Pentoxifylline possesses an antioxidant capacity proved by its ability of reducing H2O2 levels as well as by increasing motility values of stressed spermatozoa. Therefore, pentoxifylline could be recommended as antioxidant additive for spermatozoa of small ruminants under stress conditions.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42461235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.4103/2305-0500.346088
K. Manokaran, Pavithra Bhat, D. Nayak, Ravisankar Baskaran, Prabu Paramasivam, Shiek S. S. J. Ahmed, K. Priya, K. R. Ranganath Pai, Vignesh Balaji
Oxidative stress arises from an imbalance between the body’s pro-oxidants and antioxidants. Recently, oxidative stress has been proven a contributing factor to many female reproductive disorders including infertility, preeclampsia, endometriosis and polycystic ovarian syndrome. Herein, we review the mechanistic role of oxidative stress in inducing the most common female reproductive disorders. The current review has also highlighted the protective role of vitamin C, necessary for certain female reproductive hormone secretion by the antral follicle and corpus luteum and also essential for collagen production in ovarian tissue remodeling after ovulation, in alleviating oxidative stress and thus improving female reproductive outcomes.
{"title":"Oxidative stress and female reproductive disorder: A review","authors":"K. Manokaran, Pavithra Bhat, D. Nayak, Ravisankar Baskaran, Prabu Paramasivam, Shiek S. S. J. Ahmed, K. Priya, K. R. Ranganath Pai, Vignesh Balaji","doi":"10.4103/2305-0500.346088","DOIUrl":"https://doi.org/10.4103/2305-0500.346088","url":null,"abstract":"Oxidative stress arises from an imbalance between the body’s pro-oxidants and antioxidants. Recently, oxidative stress has been proven a contributing factor to many female reproductive disorders including infertility, preeclampsia, endometriosis and polycystic ovarian syndrome. Herein, we review the mechanistic role of oxidative stress in inducing the most common female reproductive disorders. The current review has also highlighted the protective role of vitamin C, necessary for certain female reproductive hormone secretion by the antral follicle and corpus luteum and also essential for collagen production in ovarian tissue remodeling after ovulation, in alleviating oxidative stress and thus improving female reproductive outcomes.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43868831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.4103/2305-0500.346091
S. Sarmadi, Ara Omranipour, E. Mirzaian, Roghayyeh Ahangari, Zahra Yazdi, A. Asgarian, Monireh Mirzaie
Objective: To describe histopathologic findings in the placentas in women with coronavirus disease 2019 (COVID-19) during pregnancy. Methods: In a cross-sectional study, 38 pregnant women with COVID-19 and undergoing delivery between March 2020 and January 2022, were included. The patients had positive polymerase chain reaction (PCR) test for SARS-CoV-2 infection and the placentas after delivery were sent for histopathologic evaluation based on the Amsterdam Placental Workshop Group Consensus Statement and assessed by two pathologists. Results: Our results showed that maternal vascular malperfusion was the most common and was present in 17 cases (44.7%). These features included accelerated villous maturation (36.8%) distal villous hypoplasia (5.3%), placental infarction (5.3%) and intervillous fibrin deposition (10.5%). Other pathologic findings included focal calcification (10.5%), intravillous congestion and hemorrhage (10.5%), sub-chorionic hemorrhage (5.3%), acute villitis, chronic histiocytic intervillositis and delayed villous maturation each in one case (2.6%). Twelve out of 38 cases showed no significant pathologic changes. Fetal outcomes included neonatal intensive care unit admission rate of 13.2%, dyspnea 31.6%, newborn’s anosmia 7.9%, intrauterine fetal demise 2.6%, asphyxia 2.6% and neonate COVID infection 5.3%. Conclusions: Microvasculopathy, as a sign of maternal vascular malperfusion, is a common finding in placentas from SARS-CoV-2 positive pregnant women in the present study. Further studies with larger sample sizes and comparative studies between COVID-19 positive and negative, as well as information from patient follow-up are suggested.
{"title":"Placental pathologies and fetal outcome in pregnant women with COVID-19: A retrospective study","authors":"S. Sarmadi, Ara Omranipour, E. Mirzaian, Roghayyeh Ahangari, Zahra Yazdi, A. Asgarian, Monireh Mirzaie","doi":"10.4103/2305-0500.346091","DOIUrl":"https://doi.org/10.4103/2305-0500.346091","url":null,"abstract":"Objective: To describe histopathologic findings in the placentas in women with coronavirus disease 2019 (COVID-19) during pregnancy. Methods: In a cross-sectional study, 38 pregnant women with COVID-19 and undergoing delivery between March 2020 and January 2022, were included. The patients had positive polymerase chain reaction (PCR) test for SARS-CoV-2 infection and the placentas after delivery were sent for histopathologic evaluation based on the Amsterdam Placental Workshop Group Consensus Statement and assessed by two pathologists. Results: Our results showed that maternal vascular malperfusion was the most common and was present in 17 cases (44.7%). These features included accelerated villous maturation (36.8%) distal villous hypoplasia (5.3%), placental infarction (5.3%) and intervillous fibrin deposition (10.5%). Other pathologic findings included focal calcification (10.5%), intravillous congestion and hemorrhage (10.5%), sub-chorionic hemorrhage (5.3%), acute villitis, chronic histiocytic intervillositis and delayed villous maturation each in one case (2.6%). Twelve out of 38 cases showed no significant pathologic changes. Fetal outcomes included neonatal intensive care unit admission rate of 13.2%, dyspnea 31.6%, newborn’s anosmia 7.9%, intrauterine fetal demise 2.6%, asphyxia 2.6% and neonate COVID infection 5.3%. Conclusions: Microvasculopathy, as a sign of maternal vascular malperfusion, is a common finding in placentas from SARS-CoV-2 positive pregnant women in the present study. Further studies with larger sample sizes and comparative studies between COVID-19 positive and negative, as well as information from patient follow-up are suggested.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44805965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.4103/2305-0500.346093
A. Mostafa, M. El‐Belely, S. Ismail, R. El-Sheshtawy, M. Shahba
Objective: To investigate the effects of non-permeable cryoprotectant, cholesterol-loaded cyclodextrin, when added at different concentrations into cooled and frozen-thawed semen extended with Tris-citrate-fructose egg yolk glycerol and lecithin-based extenders. Methods: A total of 40 ejaculates from four buffalo bulls were collected using artificial vagina. Ejaculates were extended with one of Tris-citrate-fructose egg yolk glycerol and lecithin-based extenders which contained different concentrations [0 (control), 0.75, 1.50, 2.25 and 3.00 mg/mL] of cholesterol-loaded cyclodextrin. The extended semen samples were cooled to 5 °C and then frozen slowly to -196 °C in 0.25 mL ministraws before being stored in liquid nitrogen pending its evaluation. Sperm motility, live sperm, normal sperm morphology, sperm membrane integrity and acrosome morphology were measured. Results: Supplementation of cholesterol-loaded cyclodextrin improved progressive motility, viability, morphology and acrosome as well as plasma membrane integrities at 1.50-2.25 mg/mL depending upon types of used extenders and stages of pre- and post-freezing process (P<0.01). The best concentration was 1.50 mg/mL at pre-freeze stage and 2.25 mg/mL at post- freezing. However, greater concentration (3.00 mg/mL) of cholesterol-loaded cyclodextrin had a detrimental effect compared to the control group with the two evaluated extenders (P<0.01). Conclusions: Cholesterol-loaded cyclodextrin supplementation at 1.50-2.25 mg/mL concentration could improve pre-frozen and post-thawed buffalo sperm quality. The most suitable concentration is 1.50 mg/mL at pre-freeze stage and 2.25 mg/mL at post-freezing.
{"title":"Effect of cholesterol-loaded cyclodextrin enriched extenders on the quality of prefrozen and frozen buffalo semen","authors":"A. Mostafa, M. El‐Belely, S. Ismail, R. El-Sheshtawy, M. Shahba","doi":"10.4103/2305-0500.346093","DOIUrl":"https://doi.org/10.4103/2305-0500.346093","url":null,"abstract":"Objective: To investigate the effects of non-permeable cryoprotectant, cholesterol-loaded cyclodextrin, when added at different concentrations into cooled and frozen-thawed semen extended with Tris-citrate-fructose egg yolk glycerol and lecithin-based extenders. Methods: A total of 40 ejaculates from four buffalo bulls were collected using artificial vagina. Ejaculates were extended with one of Tris-citrate-fructose egg yolk glycerol and lecithin-based extenders which contained different concentrations [0 (control), 0.75, 1.50, 2.25 and 3.00 mg/mL] of cholesterol-loaded cyclodextrin. The extended semen samples were cooled to 5 °C and then frozen slowly to -196 °C in 0.25 mL ministraws before being stored in liquid nitrogen pending its evaluation. Sperm motility, live sperm, normal sperm morphology, sperm membrane integrity and acrosome morphology were measured. Results: Supplementation of cholesterol-loaded cyclodextrin improved progressive motility, viability, morphology and acrosome as well as plasma membrane integrities at 1.50-2.25 mg/mL depending upon types of used extenders and stages of pre- and post-freezing process (P<0.01). The best concentration was 1.50 mg/mL at pre-freeze stage and 2.25 mg/mL at post- freezing. However, greater concentration (3.00 mg/mL) of cholesterol-loaded cyclodextrin had a detrimental effect compared to the control group with the two evaluated extenders (P<0.01). Conclusions: Cholesterol-loaded cyclodextrin supplementation at 1.50-2.25 mg/mL concentration could improve pre-frozen and post-thawed buffalo sperm quality. The most suitable concentration is 1.50 mg/mL at pre-freeze stage and 2.25 mg/mL at post-freezing.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45308923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.4103/2305-0500.341117
A. Fadl, E. Abdelnaby, H. El-Sherbiny
{"title":"INRA82 extender enhances semen quality in ram under cooled and cryopreserved stages","authors":"A. Fadl, E. Abdelnaby, H. El-Sherbiny","doi":"10.4103/2305-0500.341117","DOIUrl":"https://doi.org/10.4103/2305-0500.341117","url":null,"abstract":"","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48673016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.4103/2305-0500.341116
Zeinab Maleki, A. Farshad, J. Rostamzadeh
{"title":"Protective effect of Scrophularia striata combined with trehalose and cysteine added to diluents on cryopreservd goat epididymal sperm","authors":"Zeinab Maleki, A. Farshad, J. Rostamzadeh","doi":"10.4103/2305-0500.341116","DOIUrl":"https://doi.org/10.4103/2305-0500.341116","url":null,"abstract":"","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43437528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.4103/2305-0500.341115
W. Oshiegbu, C. Elu, I. Onyesom
{"title":"Profiling of seminal antioxidant indices and sperm quality in Plasmodium berghei-induced malarial mice treated with Phyllanthus amarus","authors":"W. Oshiegbu, C. Elu, I. Onyesom","doi":"10.4103/2305-0500.341115","DOIUrl":"https://doi.org/10.4103/2305-0500.341115","url":null,"abstract":"","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43165605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.4103/2305-0500.341114
Z. Djerrou, Houria Benyezzar-Kenana, Z. Maameri, L. Benhamza
{"title":"An ethnopharmacological survey of medicinal plants used in the traditional treatment of human infertility in eastern Algeria","authors":"Z. Djerrou, Houria Benyezzar-Kenana, Z. Maameri, L. Benhamza","doi":"10.4103/2305-0500.341114","DOIUrl":"https://doi.org/10.4103/2305-0500.341114","url":null,"abstract":"","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42804038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.4103/2305-0500.341112
M. Mahfuzur, Md. S. Alam, M. Billah
{"title":"Determinants of emergency contraceptive pill use in Bangladesh: An analysis of national survey data","authors":"M. Mahfuzur, Md. S. Alam, M. Billah","doi":"10.4103/2305-0500.341112","DOIUrl":"https://doi.org/10.4103/2305-0500.341112","url":null,"abstract":"","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44124700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.4103/2305-0500.341113
A. Ibikunle, P. Ajayi, O. Ibikunle, D. Ibirongbe, A. Alabi, K. Durowade
{"title":"Spousal communication, fertility preference and other factors affecting contraceptive use among married couples in Ekiti State, Nigeria","authors":"A. Ibikunle, P. Ajayi, O. Ibikunle, D. Ibirongbe, A. Alabi, K. Durowade","doi":"10.4103/2305-0500.341113","DOIUrl":"https://doi.org/10.4103/2305-0500.341113","url":null,"abstract":"","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41752978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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