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Profiling of seminal antioxidant indices and sperm quality in Plasmodium berghei-induced malarial mice treated with Phyllanthus amarus 白桦对柏氏疟原虫诱导的疟疾小鼠精液抗氧化指标及精子质量的影响
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2022-03-01 DOI: 10.4103/2305-0500.341115
W. Oshiegbu, C. Elu, I. Onyesom
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引用次数: 2
An ethnopharmacological survey of medicinal plants used in the traditional treatment of human infertility in eastern Algeria 阿尔及利亚东部用于传统治疗人类不孕不育的药用植物的民族药理学调查
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2022-03-01 DOI: 10.4103/2305-0500.341114
Z. Djerrou, Houria Benyezzar-Kenana, Z. Maameri, L. Benhamza
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引用次数: 1
Spousal communication, fertility preference and other factors affecting contraceptive use among married couples in Ekiti State, Nigeria 尼日利亚埃基提州已婚夫妇的配偶沟通、生育偏好和其他影响避孕药具使用的因素
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2022-03-01 DOI: 10.4103/2305-0500.341113
A. Ibikunle, P. Ajayi, O. Ibikunle, D. Ibirongbe, A. Alabi, K. Durowade
{"title":"Spousal communication, fertility preference and other factors affecting contraceptive use among married couples in Ekiti State, Nigeria","authors":"A. Ibikunle, P. Ajayi, O. Ibikunle, D. Ibirongbe, A. Alabi, K. Durowade","doi":"10.4103/2305-0500.341113","DOIUrl":"https://doi.org/10.4103/2305-0500.341113","url":null,"abstract":"","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"11 1","pages":"70 - 76"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41752978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determinants of emergency contraceptive pill use in Bangladesh: An analysis of national survey data 孟加拉国紧急避孕药使用的决定因素:对全国调查数据的分析
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2022-03-01 DOI: 10.4103/2305-0500.341112
M. Mahfuzur, Md. S. Alam, M. Billah
{"title":"Determinants of emergency contraceptive pill use in Bangladesh: An analysis of national survey data","authors":"M. Mahfuzur, Md. S. Alam, M. Billah","doi":"10.4103/2305-0500.341112","DOIUrl":"https://doi.org/10.4103/2305-0500.341112","url":null,"abstract":"","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"11 1","pages":"62 - 69"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44124700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A scoping review of SARS-CoV-2 and male infertility: Concerns and future prospects 严重急性呼吸系统综合征冠状病毒2型与男性不育的范围界定综述:关注点和未来前景
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2022-03-01 DOI: 10.4103/2305-0500.341111
F. Al-Khikani, Aalae Ayit
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been detected in the blood, urine, facial/anal swabs, semen, and vaginal discharge;all have been shown to contain SARSCoV-2 RNA. Recent findings have highlighted the prospect of SARS-CoV-2 invading the genital system in addition to other tissues, which might give rise to reproductive concerns. This investigation sheds light on male reproductive tract vulnerability to invasion by SARS-CoV-2 and provides a foundation for further researches into male fertility. Males are infected with COVID-19 at a higher rate than females. As a result, some data suggest that this viral infection might affect the male reproductive system. The probable causes for male genital tract abnormalities in COVID-19 are: 1) high expression of angiotensin-converting enzyme 2 in the testes;2) SARS-CoV-2 infection indirectly induces immune response in the testes;3) SARS-CoV-2 directly damages male genital cells by virus-receptor binding activity;4) fever in SARSCoV-2 infected males may cause damages to testicular cells;5) testosterone level decreased in SAR-CoV-2 infected males;6) males are more susceptible to COVID-19 than females, which may be due to differences in the physiology of the genital tract. This review seeks to offer some insights into the potential causes of COVID-19 that affect the male reproductive system, as well as future prospect on this issue.
在血液、尿液、面部/肛门拭子、精液和阴道分泌物中检测到严重急性呼吸综合征冠状病毒2型;所有这些都已被证明含有严重急性呼吸系统综合征冠状病毒2型核糖核酸。最近的研究结果强调了严重急性呼吸系统综合征冠状病毒2型除了入侵其他组织外,还可能入侵生殖系统,这可能会引起生殖问题。这项研究揭示了男性生殖道易受严重急性呼吸系统综合征冠状病毒2型侵袭的脆弱性,并为进一步研究男性生育能力奠定了基础。男性感染新冠肺炎的比率高于女性。因此,一些数据表明,这种病毒感染可能会影响男性生殖系统。新冠肺炎男性生殖道异常的可能原因是:1)睾丸中血管紧张素转化酶2的高表达;2) 严重急性呼吸系统综合征冠状病毒2型感染间接诱导睾丸免疫反应;3) 严重急性呼吸系统综合征冠状病毒2型通过病毒受体结合活性直接损害男性生殖细胞;4) 严重急性呼吸系统综合征冠状病毒2型感染的男性发烧可能导致睾丸细胞损伤;5) 严重急性呼吸系统综合征冠状病毒2型感染男性的睾酮水平下降;6) 男性比女性更容易感染新冠肺炎,这可能是由于生殖道生理学的差异。这篇综述试图对影响男性生殖系统的新冠肺炎的潜在原因以及这一问题的未来前景提供一些见解。
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引用次数: 6
Successful live birth after intracytoplasmic sperm injection using testicular sperm in non-mosaic Klinefelter syndrome 非花叶性Klinefelter综合征患者使用睾丸精子注射胞浆内精子后成功活产
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2022-01-01 DOI: 10.4103/2305-0500.335862
S. Jellad, R. Arfaoui, N. Souayah, F. Hammami
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引用次数: 0
Effects of enzymatic and non-enzymatic antioxidants in diluents on cryopreserved bull epididymal sperm 稀释剂中酶促和非酶促抗氧化剂对公牛附睾精子冷冻保存的影响
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2022-01-01 DOI: 10.4103/2305-0500.335861
Hasan Sakhdary, A. Farshad, J. Rostamzadeh, F. Binabaj, Keyvan Sobhani
Objective: To evaluate the supplementation effects of vitamin E, vitamin C, superoxide dismutase (SOD), and catalase to diluents on bull cryopreserved epididymal sperm. Methods: Sperm were retrieved from 20 bull testes and were then supplemented with 0.1 mM vitamin E, 5.0 mM vitamin C, 100.0 IU/mL SOD, and 100.0 μg/mL catalase alone, or in a combination. The control treatment contained no addition. After supplementation, samples were frozen and stored in liquid nitrogen. The sperm parameters including motility, progressive motility, viability, acrosome integrity, plasma membrane integrity, kinematics and DNA damage were evaluated following the thawing process. Results: Vitamin E alone significantly increased the parameters of acrosome and membrane integrity compared to the control treatment (P<0.05). While compared to the control treatment, vitamin C had no improvement effect on sperm characteristics except for membrane integrity. Treatment of vitamin E+vitamin C had a significant improvement in total motility, progressive motility, viability, membrane and acrosome integrity compared to the control and other treatments (P<0.05). Compared to the control treatment, addition of SOD or catalase alone significantly improved the percentages of total motility, progressive motility, viability, membrane and acrosome integrity (P<0.05). Furthermore, SOD+catalase significantly increased total motility, progressive motility, viability, acrosome and membrane integrity characteristics compared to the catalase treatment (P<0.05). Vitamin E alone, vitamin E+vitamin C, and SOD in diluents decreased DNA damages and thereby improved the rate of intact sperm heads. Conclusions: Addition of 100.0 IU/mL SOD alone and 0.1 mM vitamin E+5.0 mM vitamin C, and also 5.0 mM vitamin C+100 μg/mL catalase in a combination improves the quality of cryopreserved bull epididymal sperm and could be used for cryopreservation.
目的:评价维生素E、维生素C、超氧化物歧化酶(SOD)和过氧化氢酶对公牛冷冻保存附睾精子稀释液的补充作用。方法:从20个公牛睾丸中提取精子,然后单独或组合补充0.1mM维生素E、5.0mM维生素C、100.0IU/mL SOD和100.0μg/mL过氧化氢酶。对照处理不含任何添加剂。补充后,将样品冷冻并储存在液氮中。解冻过程后评估精子的参数,包括活力、进行性活力、活力、顶体完整性、质膜完整性、运动学和DNA损伤。结果:与对照组相比,单独使用维生素E显著提高了精子顶体参数和膜完整性(P<0.05),而与对照组比较,维生素C对精子特征的改善作用除膜完整性外没有。与对照和其他处理相比,维生素E+维生素C处理在总运动能力、进行性运动能力、活力、膜和顶体完整性方面有显著改善(P<0.05),此外,与过氧化氢酶处理相比,SOD+过氧化氢酶显著提高了总活力、进行性活力、活力、顶体和膜完整性特征(P<0.05)。单独的维生素E、维生素E+维生素C和稀释剂中的SOD减少了DNA损伤,从而提高了完整精子头的率。结论:单独添加100.0IU/mL SOD和0.1mM维生素E+5.0mM维生素C以及5.0mM维生素C+100μg/mL过氧化氢酶可提高冷冻保存公牛附睾精子的质量,可用于冷冻保存。
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引用次数: 1
Uptake of in-vitro fertilization among couples attending fertility clinic in a tertiary health institution 在三级医疗机构生育诊所就诊的夫妇接受体外受精
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2022-01-01 DOI: 10.4103/2305-0500.335857
Mabel Omobude, O. Olorunfemi, F. Okafor
Objective: To identify factors that determine the uptake of in-vitro fertilization (IVF), and to determine the predicting effect of the identified factors on the uptake of IVF among couples attending fertility clinic at a tertiary health institution in Benin City, Nigeria. Methods: This study adopted a cross-sectional descriptive design. A self-structured questionnaire tested was validated and administered to 250 couples who attended fertility clinic. Data were collected from March 2021 to June 2021 and were analyzed using the Statistical Package for Social Science (SPSS) version 21. Data were presented and interpreted using descriptive statistics and inferential statistics such as Chi-square, univariate statistics and multivariate logistic regression analysis. Results: Among 250 couples, 154 (61.6%) were willing to adopt IVF. The multivariate logistic regression analysis showed that catholic religion [odds ratio (OR) 0.21, 95% confident interval (CI) 0.54-0.73], family income (OR 1.50, 95% CI 1.10-2.00) and age (OR 1.04, 95% CI 1.01-1.10) were the major factors that determined the uptake of IVF, with P-value of <0.001, 0.018 and 0.031, respectively. Conclusions: The uptake of IVF could be sustained or improved on, if the government should improve on the cost of living for families, and religious leaders need to be encouraged to pass on the information about IVF to their members to be well guided about the possibilities of IVF.
目的:确定决定体外受精(IVF)接受的因素,并确定所确定的因素对在尼日利亚贝宁市一家三级卫生机构生育诊所就诊的夫妇接受体外受精的预测作用。方法:本研究采用横断面描述性设计。对250对到生育诊所就诊的夫妇进行了问卷调查。数据于2021年3月至2021年6月收集,并使用社会科学统计软件包(SPSS)第21版进行分析。使用描述性统计和推理统计(如卡方统计、单变量统计和多变量逻辑回归分析)来呈现和解释数据。结果:250对夫妇中,有154对(61.6%)愿意采用体外受精。多因素logistic回归分析显示,天主教信仰[比值比(OR) 0.21, 95%可信区间(CI) 0.54 ~ 0.73]、家庭收入(OR 1.50, 95% CI 1.10 ~ 2.00)和年龄(OR 1.04, 95% CI 1.01 ~ 1.10)是决定接受体外受精的主要因素,p值分别<0.001、0.018和0.031。结论:只要政府提高家庭生活成本,鼓励宗教领袖向其成员传递有关试管婴儿的信息,引导他们了解试管婴儿的可能性,试管婴儿的采用是可以持续或提高的。
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引用次数: 2
Taurine in semen extender modulates post-thaw semen quality, sperm kinematics and oxidative stress status in mithun (Bos frontalis) spermatozoa 精液扩展剂中的牛磺酸调节解冻后精子质量、精子运动和氧化应激状态
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2022-01-01 DOI: 10.4103/2305-0500.335860
Perumal Ponraj, K. Khate, K. Vupru
Objective: To assess the effect of taurine on post-thaw semen quality parameters, sperm kinematics, antioxidant and oxidative stress profiles and sperm cholesterol efflux in mithun (Bos frontalis). Methods: A total of 50 ejaculates (n=25 samples) were selected based on biophysical parameters. Each sample was split into four equal aliquots after dilution with the Tris-citrate-glycerol extender. Group I, II, III and IV contained 0 mM (the control), 25 mM, 50 mM and 100 mM of taurine, respectively. Frozen-thawed samples were analysed for motility parameters (progressive forward and in bovine cervical mucus penetration test), kinetic and velocity parameters by computer-assisted sperm analyzer, viability, sperm and nuclear abnormalities, acrosome integrity, plasma membrane and nuclear integrities, sperm enzymatic leakage and biochemical (sperm cholesterol and oxidative stress) profiles. Results: The extender containing 50 mM taurine led to a significant enhancement in viability, acrosomal integrity, plasma membrane integrity, motility (progressive and in cervical mucus), and sperm cholesterol content and notably reduced sperm morphological and nuclear abnormalities, and leakage of intracellular enzymes compared to other taurine treated and untreated control groups (P<0.05). Moreover, in addition to significant improvement in kinetic and velocity profiles, 50 mM taurine protected the integrity of acrosome and biochemical membranes than in the untreated control and other taurine treated groups. Inclusion of 50 mM taurine held a clear advantage over the control or 25 mM or 100 mM taurine in cryopreservation of mithun semen. Conclusions: Taurine (50 mM) supplementation in semen extender can be effectively utilized to reduce oxidative stress and improve post-thaw semen quality in mithun.
目的:探讨牛磺酸对山羊解冻后精液质量参数、精子运动学、抗氧化应激及精子胆固醇外排的影响。方法:根据生物物理参数选取50例射精(n=25例)。用三柠檬酸盐-甘油扩展剂稀释后,将每个样品分成四等份。ⅰ组、ⅱ组、ⅲ组和ⅳ组牛磺酸含量分别为0 mM(对照)、25 mM、50 mM和100 mM。通过计算机辅助精子分析仪分析冻融样品的运动参数(渐进式向前和牛宫颈粘液渗透试验)、动力学和速度参数、活力、精子和核异常、顶体完整性、质膜和核完整性、精子酶泄漏和生化(精子胆固醇和氧化应激)谱。结果:与其他牛磺酸处理组和未处理组相比,添加50 mM牛磺酸的延长剂显著提高了精子活力、顶体完整性、质膜完整性、运动性(进行性和宫颈粘液)和精子胆固醇含量,显著减少了精子形态和核异常以及细胞内酶渗漏(P<0.05)。此外,与未处理的对照组和其他牛磺酸处理组相比,50 mM牛磺酸除了显著改善了动力学和速度剖面外,还保护了顶体和生化膜的完整性。在低温保存中,加入50毫米牛磺酸比对照组或25毫米或100毫米牛磺酸有明显的优势。结论:在精液填充剂中添加50 mM牛磺酸可有效降低氧化应激,提高解冻后精液质量。
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引用次数: 1
Blepharis persica increases testosterone biosynthesis by modulating StAR and 3β-HSD expression in rat testicular tissues 桃通过调节大鼠睾丸组织StAR和3β-HSD的表达来增加睾酮的生物合成
Q4 REPRODUCTIVE BIOLOGY Pub Date : 2022-01-01 DOI: 10.4103/2305-0500.335859
N. Gaikar, Nishit D Patel, Samir G Patel, Priyal Patel, P. Chudasama, M. Raval
Objective: To evaluate the effect of methanolic extract and ethyl acetate fraction of methanol extract prepared from the seeds of Blepharis (B.) persica on testosterone biosynthesis and also to elucidate the underlying mechanism. Methods: Forty-eight male Wistar rats were divided into eight groups (n=6 per group). Group I received 0.3% w/w gum acacia suspension p.o. and served as the normal control group. Group II was administered testosterone propionate in arachis oil i.m. as the positive control group. Group III to V received B. persica methanolic extract p.o. at doses of 50, 100 and 200 mg/kg body weight. Group VI to VIII received B. persica ethyl acetate fraction p.o. at doses of 50, 100 and 200 mg/kg body weight. The testis was used for biochemical estimation and histological studies. The effects of methanolic extract and ethyl acetate fraction of B. persica on testicular testosterone, mRNA expression corresponding to steroidogenic acute regulatory protein (StAR) and 3β-hydroxysteroid dehydrogenase (3β-HSD) along with 3β-HSD enzyme assay were evaluated in testicular tissues and sperm concentration. Ethyl acetate fraction of B. persica was subjected to column chromatography. In-vitro studies were performed using TM3 cell line at three dose levels (50, 100, 200 μg/mL), each for methanolic extract, ethyl acetate fraction and 2-benzoxazolinone for evaluation of their comparative effect on testosterone production. Results: Ethyl acetate fraction and methanolic extract of B. persica could elevate the testicular testosterone content compared to the normal control group. The treatment with methanolic extract and ethyl acetate fraction of B. persica increased the expression of mRNA corresponding to StAR by 6.7 fold and 10.6 fold, respectively, whereas the mRNA expression of 3β-HSD increased by 5.7 fold and 7.3 fold, respectively. Moreover, fraction and extract treatment exhibited increased 3β-HSD activity in the testicular tissues and were found to elevate sperm concentration in seminal fluid. The spermatogenic potential was further ensured by histological observations. 2-benzoxazolinone was isolated from ethyl acetate fraction and identified using spectral studies. It showed the ability to increase the testosterone content in the TM3 Leydig cells. Conclusions: Methanolic extract and ethyl acetate fraction of B. persica are able to increase the testicular testosterone in rats by elevating mRNA expression of StAR and 3β-HSD in testicular tissues, leading to increase the sperm concentration.
目的:评价桃籽甲醇提取物和甲醇提取物乙酸乙酯部分对睾酮生物合成的影响,并阐明其潜在机制。方法:48只雄性Wistar大鼠分为8组(每组6只)。第一组口服0.3%w/w阿拉伯树胶混悬液,作为正常对照组。第二组为阳性对照组,于蛛网膜下腔注射丙酸睾酮。第III组至第V组以50、100和200毫克/公斤体重的剂量口服波斯芽孢杆菌甲醇提取物。第VI至VIII组以50mg/kg、100mg/kg和200mg/kg体重的剂量口服施用B.persica乙酸乙酯部分。睾丸用于生化评估和组织学研究。在睾丸组织和精子浓度中,评价了柿子甲醇提取物和乙酸乙酯部分对睾丸睾酮、甾体生成急性调节蛋白(StAR)和3β-羟基类固醇脱氢酶(3β-HSD)mRNA表达以及3β-HSD酶测定的影响。用柱色谱法测定了波斯B.persica的乙酸乙酯部分。使用TM3细胞系在三个剂量水平(50、100、200μg/mL)下进行体外研究,分别针对甲醇提取物、乙酸乙酯部分和2-苯并恶唑啉酮,以评估其对睾酮产生的比较影响。结果:与正常对照组相比,柿子乙酸乙酯组分和甲醇提取物可提高睾丸睾酮含量。用B.persica的甲醇提取物和乙酸乙酯部分处理后,StAR对应的mRNA表达分别增加了6.7倍和10.6倍,而3β-HSD的mRNA表达则分别增加了5.7倍和7.3倍。此外,组分和提取物处理显示睾丸组织中3β-HSD活性增加,并发现提高精液中精子浓度。组织学观察进一步确保了生精潜能。2-苯并恶唑啉酮从乙酸乙酯馏分中分离,并通过光谱研究进行鉴定。它显示出增加TM3 Leydig细胞中睾酮含量的能力。结论:柿子甲醇提取物和乙酸乙酯组分能够通过提高睾丸组织StAR和3β-HSD的mRNA表达来增加大鼠睾丸睾酮,从而提高精子浓度。
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引用次数: 1
期刊
Asian Pacific Journal of Reproduction
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