Pub Date : 2022-03-01DOI: 10.4103/2305-0500.341115
W. Oshiegbu, C. Elu, I. Onyesom
{"title":"Profiling of seminal antioxidant indices and sperm quality in Plasmodium berghei-induced malarial mice treated with Phyllanthus amarus","authors":"W. Oshiegbu, C. Elu, I. Onyesom","doi":"10.4103/2305-0500.341115","DOIUrl":"https://doi.org/10.4103/2305-0500.341115","url":null,"abstract":"","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"11 1","pages":"84 - 92"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43165605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.4103/2305-0500.341114
Z. Djerrou, Houria Benyezzar-Kenana, Z. Maameri, L. Benhamza
{"title":"An ethnopharmacological survey of medicinal plants used in the traditional treatment of human infertility in eastern Algeria","authors":"Z. Djerrou, Houria Benyezzar-Kenana, Z. Maameri, L. Benhamza","doi":"10.4103/2305-0500.341114","DOIUrl":"https://doi.org/10.4103/2305-0500.341114","url":null,"abstract":"","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"11 1","pages":"77 - 83"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42804038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.4103/2305-0500.341113
A. Ibikunle, P. Ajayi, O. Ibikunle, D. Ibirongbe, A. Alabi, K. Durowade
{"title":"Spousal communication, fertility preference and other factors affecting contraceptive use among married couples in Ekiti State, Nigeria","authors":"A. Ibikunle, P. Ajayi, O. Ibikunle, D. Ibirongbe, A. Alabi, K. Durowade","doi":"10.4103/2305-0500.341113","DOIUrl":"https://doi.org/10.4103/2305-0500.341113","url":null,"abstract":"","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"11 1","pages":"70 - 76"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41752978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.4103/2305-0500.341112
M. Mahfuzur, Md. S. Alam, M. Billah
{"title":"Determinants of emergency contraceptive pill use in Bangladesh: An analysis of national survey data","authors":"M. Mahfuzur, Md. S. Alam, M. Billah","doi":"10.4103/2305-0500.341112","DOIUrl":"https://doi.org/10.4103/2305-0500.341112","url":null,"abstract":"","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"11 1","pages":"62 - 69"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44124700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.4103/2305-0500.341111
F. Al-Khikani, Aalae Ayit
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been detected in the blood, urine, facial/anal swabs, semen, and vaginal discharge;all have been shown to contain SARSCoV-2 RNA. Recent findings have highlighted the prospect of SARS-CoV-2 invading the genital system in addition to other tissues, which might give rise to reproductive concerns. This investigation sheds light on male reproductive tract vulnerability to invasion by SARS-CoV-2 and provides a foundation for further researches into male fertility. Males are infected with COVID-19 at a higher rate than females. As a result, some data suggest that this viral infection might affect the male reproductive system. The probable causes for male genital tract abnormalities in COVID-19 are: 1) high expression of angiotensin-converting enzyme 2 in the testes;2) SARS-CoV-2 infection indirectly induces immune response in the testes;3) SARS-CoV-2 directly damages male genital cells by virus-receptor binding activity;4) fever in SARSCoV-2 infected males may cause damages to testicular cells;5) testosterone level decreased in SAR-CoV-2 infected males;6) males are more susceptible to COVID-19 than females, which may be due to differences in the physiology of the genital tract. This review seeks to offer some insights into the potential causes of COVID-19 that affect the male reproductive system, as well as future prospect on this issue.
{"title":"A scoping review of SARS-CoV-2 and male infertility: Concerns and future prospects","authors":"F. Al-Khikani, Aalae Ayit","doi":"10.4103/2305-0500.341111","DOIUrl":"https://doi.org/10.4103/2305-0500.341111","url":null,"abstract":"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been detected in the blood, urine, facial/anal swabs, semen, and vaginal discharge;all have been shown to contain SARSCoV-2 RNA. Recent findings have highlighted the prospect of SARS-CoV-2 invading the genital system in addition to other tissues, which might give rise to reproductive concerns. This investigation sheds light on male reproductive tract vulnerability to invasion by SARS-CoV-2 and provides a foundation for further researches into male fertility. Males are infected with COVID-19 at a higher rate than females. As a result, some data suggest that this viral infection might affect the male reproductive system. The probable causes for male genital tract abnormalities in COVID-19 are: 1) high expression of angiotensin-converting enzyme 2 in the testes;2) SARS-CoV-2 infection indirectly induces immune response in the testes;3) SARS-CoV-2 directly damages male genital cells by virus-receptor binding activity;4) fever in SARSCoV-2 infected males may cause damages to testicular cells;5) testosterone level decreased in SAR-CoV-2 infected males;6) males are more susceptible to COVID-19 than females, which may be due to differences in the physiology of the genital tract. This review seeks to offer some insights into the potential causes of COVID-19 that affect the male reproductive system, as well as future prospect on this issue.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"11 1","pages":"53 - 61"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44906789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4103/2305-0500.335862
S. Jellad, R. Arfaoui, N. Souayah, F. Hammami
{"title":"Successful live birth after intracytoplasmic sperm injection using testicular sperm in non-mosaic Klinefelter syndrome","authors":"S. Jellad, R. Arfaoui, N. Souayah, F. Hammami","doi":"10.4103/2305-0500.335862","DOIUrl":"https://doi.org/10.4103/2305-0500.335862","url":null,"abstract":"","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"11 1","pages":"51 - 52"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45673283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4103/2305-0500.335861
Hasan Sakhdary, A. Farshad, J. Rostamzadeh, F. Binabaj, Keyvan Sobhani
Objective: To evaluate the supplementation effects of vitamin E, vitamin C, superoxide dismutase (SOD), and catalase to diluents on bull cryopreserved epididymal sperm. Methods: Sperm were retrieved from 20 bull testes and were then supplemented with 0.1 mM vitamin E, 5.0 mM vitamin C, 100.0 IU/mL SOD, and 100.0 μg/mL catalase alone, or in a combination. The control treatment contained no addition. After supplementation, samples were frozen and stored in liquid nitrogen. The sperm parameters including motility, progressive motility, viability, acrosome integrity, plasma membrane integrity, kinematics and DNA damage were evaluated following the thawing process. Results: Vitamin E alone significantly increased the parameters of acrosome and membrane integrity compared to the control treatment (P<0.05). While compared to the control treatment, vitamin C had no improvement effect on sperm characteristics except for membrane integrity. Treatment of vitamin E+vitamin C had a significant improvement in total motility, progressive motility, viability, membrane and acrosome integrity compared to the control and other treatments (P<0.05). Compared to the control treatment, addition of SOD or catalase alone significantly improved the percentages of total motility, progressive motility, viability, membrane and acrosome integrity (P<0.05). Furthermore, SOD+catalase significantly increased total motility, progressive motility, viability, acrosome and membrane integrity characteristics compared to the catalase treatment (P<0.05). Vitamin E alone, vitamin E+vitamin C, and SOD in diluents decreased DNA damages and thereby improved the rate of intact sperm heads. Conclusions: Addition of 100.0 IU/mL SOD alone and 0.1 mM vitamin E+5.0 mM vitamin C, and also 5.0 mM vitamin C+100 μg/mL catalase in a combination improves the quality of cryopreserved bull epididymal sperm and could be used for cryopreservation.
{"title":"Effects of enzymatic and non-enzymatic antioxidants in diluents on cryopreserved bull epididymal sperm","authors":"Hasan Sakhdary, A. Farshad, J. Rostamzadeh, F. Binabaj, Keyvan Sobhani","doi":"10.4103/2305-0500.335861","DOIUrl":"https://doi.org/10.4103/2305-0500.335861","url":null,"abstract":"Objective: To evaluate the supplementation effects of vitamin E, vitamin C, superoxide dismutase (SOD), and catalase to diluents on bull cryopreserved epididymal sperm. Methods: Sperm were retrieved from 20 bull testes and were then supplemented with 0.1 mM vitamin E, 5.0 mM vitamin C, 100.0 IU/mL SOD, and 100.0 μg/mL catalase alone, or in a combination. The control treatment contained no addition. After supplementation, samples were frozen and stored in liquid nitrogen. The sperm parameters including motility, progressive motility, viability, acrosome integrity, plasma membrane integrity, kinematics and DNA damage were evaluated following the thawing process. Results: Vitamin E alone significantly increased the parameters of acrosome and membrane integrity compared to the control treatment (P<0.05). While compared to the control treatment, vitamin C had no improvement effect on sperm characteristics except for membrane integrity. Treatment of vitamin E+vitamin C had a significant improvement in total motility, progressive motility, viability, membrane and acrosome integrity compared to the control and other treatments (P<0.05). Compared to the control treatment, addition of SOD or catalase alone significantly improved the percentages of total motility, progressive motility, viability, membrane and acrosome integrity (P<0.05). Furthermore, SOD+catalase significantly increased total motility, progressive motility, viability, acrosome and membrane integrity characteristics compared to the catalase treatment (P<0.05). Vitamin E alone, vitamin E+vitamin C, and SOD in diluents decreased DNA damages and thereby improved the rate of intact sperm heads. Conclusions: Addition of 100.0 IU/mL SOD alone and 0.1 mM vitamin E+5.0 mM vitamin C, and also 5.0 mM vitamin C+100 μg/mL catalase in a combination improves the quality of cryopreserved bull epididymal sperm and could be used for cryopreservation.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"11 1","pages":"44 - 50"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46307480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4103/2305-0500.335857
Mabel Omobude, O. Olorunfemi, F. Okafor
Objective: To identify factors that determine the uptake of in-vitro fertilization (IVF), and to determine the predicting effect of the identified factors on the uptake of IVF among couples attending fertility clinic at a tertiary health institution in Benin City, Nigeria. Methods: This study adopted a cross-sectional descriptive design. A self-structured questionnaire tested was validated and administered to 250 couples who attended fertility clinic. Data were collected from March 2021 to June 2021 and were analyzed using the Statistical Package for Social Science (SPSS) version 21. Data were presented and interpreted using descriptive statistics and inferential statistics such as Chi-square, univariate statistics and multivariate logistic regression analysis. Results: Among 250 couples, 154 (61.6%) were willing to adopt IVF. The multivariate logistic regression analysis showed that catholic religion [odds ratio (OR) 0.21, 95% confident interval (CI) 0.54-0.73], family income (OR 1.50, 95% CI 1.10-2.00) and age (OR 1.04, 95% CI 1.01-1.10) were the major factors that determined the uptake of IVF, with P-value of <0.001, 0.018 and 0.031, respectively. Conclusions: The uptake of IVF could be sustained or improved on, if the government should improve on the cost of living for families, and religious leaders need to be encouraged to pass on the information about IVF to their members to be well guided about the possibilities of IVF.
目的:确定决定体外受精(IVF)接受的因素,并确定所确定的因素对在尼日利亚贝宁市一家三级卫生机构生育诊所就诊的夫妇接受体外受精的预测作用。方法:本研究采用横断面描述性设计。对250对到生育诊所就诊的夫妇进行了问卷调查。数据于2021年3月至2021年6月收集,并使用社会科学统计软件包(SPSS)第21版进行分析。使用描述性统计和推理统计(如卡方统计、单变量统计和多变量逻辑回归分析)来呈现和解释数据。结果:250对夫妇中,有154对(61.6%)愿意采用体外受精。多因素logistic回归分析显示,天主教信仰[比值比(OR) 0.21, 95%可信区间(CI) 0.54 ~ 0.73]、家庭收入(OR 1.50, 95% CI 1.10 ~ 2.00)和年龄(OR 1.04, 95% CI 1.01 ~ 1.10)是决定接受体外受精的主要因素,p值分别<0.001、0.018和0.031。结论:只要政府提高家庭生活成本,鼓励宗教领袖向其成员传递有关试管婴儿的信息,引导他们了解试管婴儿的可能性,试管婴儿的采用是可以持续或提高的。
{"title":"Uptake of in-vitro fertilization among couples attending fertility clinic in a tertiary health institution","authors":"Mabel Omobude, O. Olorunfemi, F. Okafor","doi":"10.4103/2305-0500.335857","DOIUrl":"https://doi.org/10.4103/2305-0500.335857","url":null,"abstract":"Objective: To identify factors that determine the uptake of in-vitro fertilization (IVF), and to determine the predicting effect of the identified factors on the uptake of IVF among couples attending fertility clinic at a tertiary health institution in Benin City, Nigeria. Methods: This study adopted a cross-sectional descriptive design. A self-structured questionnaire tested was validated and administered to 250 couples who attended fertility clinic. Data were collected from March 2021 to June 2021 and were analyzed using the Statistical Package for Social Science (SPSS) version 21. Data were presented and interpreted using descriptive statistics and inferential statistics such as Chi-square, univariate statistics and multivariate logistic regression analysis. Results: Among 250 couples, 154 (61.6%) were willing to adopt IVF. The multivariate logistic regression analysis showed that catholic religion [odds ratio (OR) 0.21, 95% confident interval (CI) 0.54-0.73], family income (OR 1.50, 95% CI 1.10-2.00) and age (OR 1.04, 95% CI 1.01-1.10) were the major factors that determined the uptake of IVF, with P-value of <0.001, 0.018 and 0.031, respectively. Conclusions: The uptake of IVF could be sustained or improved on, if the government should improve on the cost of living for families, and religious leaders need to be encouraged to pass on the information about IVF to their members to be well guided about the possibilities of IVF.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"11 1","pages":"12 - 19"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46347533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4103/2305-0500.335860
Perumal Ponraj, K. Khate, K. Vupru
Objective: To assess the effect of taurine on post-thaw semen quality parameters, sperm kinematics, antioxidant and oxidative stress profiles and sperm cholesterol efflux in mithun (Bos frontalis). Methods: A total of 50 ejaculates (n=25 samples) were selected based on biophysical parameters. Each sample was split into four equal aliquots after dilution with the Tris-citrate-glycerol extender. Group I, II, III and IV contained 0 mM (the control), 25 mM, 50 mM and 100 mM of taurine, respectively. Frozen-thawed samples were analysed for motility parameters (progressive forward and in bovine cervical mucus penetration test), kinetic and velocity parameters by computer-assisted sperm analyzer, viability, sperm and nuclear abnormalities, acrosome integrity, plasma membrane and nuclear integrities, sperm enzymatic leakage and biochemical (sperm cholesterol and oxidative stress) profiles. Results: The extender containing 50 mM taurine led to a significant enhancement in viability, acrosomal integrity, plasma membrane integrity, motility (progressive and in cervical mucus), and sperm cholesterol content and notably reduced sperm morphological and nuclear abnormalities, and leakage of intracellular enzymes compared to other taurine treated and untreated control groups (P<0.05). Moreover, in addition to significant improvement in kinetic and velocity profiles, 50 mM taurine protected the integrity of acrosome and biochemical membranes than in the untreated control and other taurine treated groups. Inclusion of 50 mM taurine held a clear advantage over the control or 25 mM or 100 mM taurine in cryopreservation of mithun semen. Conclusions: Taurine (50 mM) supplementation in semen extender can be effectively utilized to reduce oxidative stress and improve post-thaw semen quality in mithun.
{"title":"Taurine in semen extender modulates post-thaw semen quality, sperm kinematics and oxidative stress status in mithun (Bos frontalis) spermatozoa","authors":"Perumal Ponraj, K. Khate, K. Vupru","doi":"10.4103/2305-0500.335860","DOIUrl":"https://doi.org/10.4103/2305-0500.335860","url":null,"abstract":"Objective: To assess the effect of taurine on post-thaw semen quality parameters, sperm kinematics, antioxidant and oxidative stress profiles and sperm cholesterol efflux in mithun (Bos frontalis). Methods: A total of 50 ejaculates (n=25 samples) were selected based on biophysical parameters. Each sample was split into four equal aliquots after dilution with the Tris-citrate-glycerol extender. Group I, II, III and IV contained 0 mM (the control), 25 mM, 50 mM and 100 mM of taurine, respectively. Frozen-thawed samples were analysed for motility parameters (progressive forward and in bovine cervical mucus penetration test), kinetic and velocity parameters by computer-assisted sperm analyzer, viability, sperm and nuclear abnormalities, acrosome integrity, plasma membrane and nuclear integrities, sperm enzymatic leakage and biochemical (sperm cholesterol and oxidative stress) profiles. Results: The extender containing 50 mM taurine led to a significant enhancement in viability, acrosomal integrity, plasma membrane integrity, motility (progressive and in cervical mucus), and sperm cholesterol content and notably reduced sperm morphological and nuclear abnormalities, and leakage of intracellular enzymes compared to other taurine treated and untreated control groups (P<0.05). Moreover, in addition to significant improvement in kinetic and velocity profiles, 50 mM taurine protected the integrity of acrosome and biochemical membranes than in the untreated control and other taurine treated groups. Inclusion of 50 mM taurine held a clear advantage over the control or 25 mM or 100 mM taurine in cryopreservation of mithun semen. Conclusions: Taurine (50 mM) supplementation in semen extender can be effectively utilized to reduce oxidative stress and improve post-thaw semen quality in mithun.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"11 1","pages":"35 - 43"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45628121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4103/2305-0500.335859
N. Gaikar, Nishit D Patel, Samir G Patel, Priyal Patel, P. Chudasama, M. Raval
Objective: To evaluate the effect of methanolic extract and ethyl acetate fraction of methanol extract prepared from the seeds of Blepharis (B.) persica on testosterone biosynthesis and also to elucidate the underlying mechanism. Methods: Forty-eight male Wistar rats were divided into eight groups (n=6 per group). Group I received 0.3% w/w gum acacia suspension p.o. and served as the normal control group. Group II was administered testosterone propionate in arachis oil i.m. as the positive control group. Group III to V received B. persica methanolic extract p.o. at doses of 50, 100 and 200 mg/kg body weight. Group VI to VIII received B. persica ethyl acetate fraction p.o. at doses of 50, 100 and 200 mg/kg body weight. The testis was used for biochemical estimation and histological studies. The effects of methanolic extract and ethyl acetate fraction of B. persica on testicular testosterone, mRNA expression corresponding to steroidogenic acute regulatory protein (StAR) and 3β-hydroxysteroid dehydrogenase (3β-HSD) along with 3β-HSD enzyme assay were evaluated in testicular tissues and sperm concentration. Ethyl acetate fraction of B. persica was subjected to column chromatography. In-vitro studies were performed using TM3 cell line at three dose levels (50, 100, 200 μg/mL), each for methanolic extract, ethyl acetate fraction and 2-benzoxazolinone for evaluation of their comparative effect on testosterone production. Results: Ethyl acetate fraction and methanolic extract of B. persica could elevate the testicular testosterone content compared to the normal control group. The treatment with methanolic extract and ethyl acetate fraction of B. persica increased the expression of mRNA corresponding to StAR by 6.7 fold and 10.6 fold, respectively, whereas the mRNA expression of 3β-HSD increased by 5.7 fold and 7.3 fold, respectively. Moreover, fraction and extract treatment exhibited increased 3β-HSD activity in the testicular tissues and were found to elevate sperm concentration in seminal fluid. The spermatogenic potential was further ensured by histological observations. 2-benzoxazolinone was isolated from ethyl acetate fraction and identified using spectral studies. It showed the ability to increase the testosterone content in the TM3 Leydig cells. Conclusions: Methanolic extract and ethyl acetate fraction of B. persica are able to increase the testicular testosterone in rats by elevating mRNA expression of StAR and 3β-HSD in testicular tissues, leading to increase the sperm concentration.
{"title":"Blepharis persica increases testosterone biosynthesis by modulating StAR and 3β-HSD expression in rat testicular tissues","authors":"N. Gaikar, Nishit D Patel, Samir G Patel, Priyal Patel, P. Chudasama, M. Raval","doi":"10.4103/2305-0500.335859","DOIUrl":"https://doi.org/10.4103/2305-0500.335859","url":null,"abstract":"Objective: To evaluate the effect of methanolic extract and ethyl acetate fraction of methanol extract prepared from the seeds of Blepharis (B.) persica on testosterone biosynthesis and also to elucidate the underlying mechanism. Methods: Forty-eight male Wistar rats were divided into eight groups (n=6 per group). Group I received 0.3% w/w gum acacia suspension p.o. and served as the normal control group. Group II was administered testosterone propionate in arachis oil i.m. as the positive control group. Group III to V received B. persica methanolic extract p.o. at doses of 50, 100 and 200 mg/kg body weight. Group VI to VIII received B. persica ethyl acetate fraction p.o. at doses of 50, 100 and 200 mg/kg body weight. The testis was used for biochemical estimation and histological studies. The effects of methanolic extract and ethyl acetate fraction of B. persica on testicular testosterone, mRNA expression corresponding to steroidogenic acute regulatory protein (StAR) and 3β-hydroxysteroid dehydrogenase (3β-HSD) along with 3β-HSD enzyme assay were evaluated in testicular tissues and sperm concentration. Ethyl acetate fraction of B. persica was subjected to column chromatography. In-vitro studies were performed using TM3 cell line at three dose levels (50, 100, 200 μg/mL), each for methanolic extract, ethyl acetate fraction and 2-benzoxazolinone for evaluation of their comparative effect on testosterone production. Results: Ethyl acetate fraction and methanolic extract of B. persica could elevate the testicular testosterone content compared to the normal control group. The treatment with methanolic extract and ethyl acetate fraction of B. persica increased the expression of mRNA corresponding to StAR by 6.7 fold and 10.6 fold, respectively, whereas the mRNA expression of 3β-HSD increased by 5.7 fold and 7.3 fold, respectively. Moreover, fraction and extract treatment exhibited increased 3β-HSD activity in the testicular tissues and were found to elevate sperm concentration in seminal fluid. The spermatogenic potential was further ensured by histological observations. 2-benzoxazolinone was isolated from ethyl acetate fraction and identified using spectral studies. It showed the ability to increase the testosterone content in the TM3 Leydig cells. Conclusions: Methanolic extract and ethyl acetate fraction of B. persica are able to increase the testicular testosterone in rats by elevating mRNA expression of StAR and 3β-HSD in testicular tissues, leading to increase the sperm concentration.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"11 1","pages":"27 - 34"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42304015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}