首页 > 最新文献

Lab on a Chip最新文献

英文 中文
Enabling the recirculation of leukocytes in a high-throughput microphysiological system (MPS) to study immune cell-vascular tissue interactions. 使白细胞在高通量微生理系统(MPS)中的再循环能够研究免疫细胞-血管组织的相互作用。
IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-01-06 DOI: 10.1039/d5lc01001b
Tyler Gerhardson,Nerses J Haroutunian,Ryan Dubay,Joseph N Urban,Anthony Quinnert,Brett C Isenberg,Samuel H Kann,Halee Kim,Robert Gaibler,Hesham Azizgolshani,Elizabeth L Wiellette,Corin Williams
Microphysiological systems (MPS) are promising technologies that can enhance the drug development pipeline and fill gaps in identifying medical countermeasures for a variety of public health contexts. The integration of immune cells with MPS is increasingly recognized as a critical element for accurately modeling inflammatory responses in disease, injury, and infection. Specifically, the recruitment of circulating leukocytes to the vascular endothelium is an important first step in the inflammatory cascade. However, developing an MPS that supports physiologically relevant immune cell circulation poses significant biological and engineering challenges due to the delicate, short-lived nature of immune cells and the physical stresses imparted by many pumping systems. Here we present advancements to a previously established high-throughput MPS platform, PREDICT96, to enable recirculation of neutrophil-rich flow within microfluidics-based vascular tissue models. To maintain cells in suspension during recirculation, density adjustments to the culture media were made. Hardware and software controls were integrated to develop a pumping strategy that reduced the peak velocity and acceleration on the recirculating cells, maintaining high viability (90%) and minimal activation of neutrophils for up to 24 hours of continuous recirculation through vascular tissue models. Additionally, an analytical model was developed that mapped pump configuration changes to altered flow characteristics through the system. These technical advancements will enable more accurate modeling of immune cell interactions with tissues in a high-throughput testing platform, which will enhance the understanding of and ability to respond to a range of human health threats.
微生理系统(MPS)是一种很有前途的技术,可以增强药物开发管道,填补在各种公共卫生环境中确定医疗对策方面的空白。免疫细胞与MPS的整合越来越被认为是准确模拟疾病、损伤和感染中的炎症反应的关键因素。具体来说,循环白细胞向血管内皮的募集是炎症级联反应中重要的第一步。然而,开发一种支持生理相关免疫细胞循环的MPS面临着重大的生物学和工程学挑战,因为免疫细胞的脆弱、短暂性和许多泵送系统所带来的物理压力。在这里,我们介绍了先前建立的高通量MPS平台PREDICT96的进展,该平台可以在基于微流体的血管组织模型中实现富含中性粒细胞的血流再循环。为了使细胞在循环过程中保持悬浮状态,对培养基进行了密度调整。将硬件和软件控制集成在一起,开发出一种泵送策略,可以降低再循环细胞的峰值速度和加速度,在长达24小时的血管组织模型中保持高活力(90%)和最小的中性粒细胞激活。此外,开发了一个分析模型,将泵配置的变化映射到系统中流动特性的变化。这些技术进步将使高通量测试平台能够更准确地模拟免疫细胞与组织的相互作用,这将增强对一系列人类健康威胁的理解和应对能力。
{"title":"Enabling the recirculation of leukocytes in a high-throughput microphysiological system (MPS) to study immune cell-vascular tissue interactions.","authors":"Tyler Gerhardson,Nerses J Haroutunian,Ryan Dubay,Joseph N Urban,Anthony Quinnert,Brett C Isenberg,Samuel H Kann,Halee Kim,Robert Gaibler,Hesham Azizgolshani,Elizabeth L Wiellette,Corin Williams","doi":"10.1039/d5lc01001b","DOIUrl":"https://doi.org/10.1039/d5lc01001b","url":null,"abstract":"Microphysiological systems (MPS) are promising technologies that can enhance the drug development pipeline and fill gaps in identifying medical countermeasures for a variety of public health contexts. The integration of immune cells with MPS is increasingly recognized as a critical element for accurately modeling inflammatory responses in disease, injury, and infection. Specifically, the recruitment of circulating leukocytes to the vascular endothelium is an important first step in the inflammatory cascade. However, developing an MPS that supports physiologically relevant immune cell circulation poses significant biological and engineering challenges due to the delicate, short-lived nature of immune cells and the physical stresses imparted by many pumping systems. Here we present advancements to a previously established high-throughput MPS platform, PREDICT96, to enable recirculation of neutrophil-rich flow within microfluidics-based vascular tissue models. To maintain cells in suspension during recirculation, density adjustments to the culture media were made. Hardware and software controls were integrated to develop a pumping strategy that reduced the peak velocity and acceleration on the recirculating cells, maintaining high viability (90%) and minimal activation of neutrophils for up to 24 hours of continuous recirculation through vascular tissue models. Additionally, an analytical model was developed that mapped pump configuration changes to altered flow characteristics through the system. These technical advancements will enable more accurate modeling of immune cell interactions with tissues in a high-throughput testing platform, which will enhance the understanding of and ability to respond to a range of human health threats.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"11 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145907541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High-throughput biomimetic cycling of red blood cells: elucidating the morpho-mechanical determinants of fatigue and clearance 红细胞的高通量仿生循环:阐明疲劳和清除的形态-机械决定因素
IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-01-05 DOI: 10.1039/D5LC01022E
Yahui Du, Wenjiao Wu, Yuexiu Chen, Lihang Zhu, Shuhao Ma, Fengjiang Zhang and Xuejin Li

During their 120-day circulatory lifespan, red blood cells (RBCs) undergo repeated mechanical deformation as they traverse microcapillaries and splenic inter-endothelial slits (IES). This cyclic mechanical loading gradually impairs RBC deformability, ultimately leading to their clearance by the spleen. However, current platforms for investigating RBC fatigue often couple mechanical loading with real-time observation, which obscures the cumulative impact of cyclic strain. To address this limitation, we developed an integrated microfluidic chip equipped with a dedicated “S”-shaped fatigue zone–each RBC experiences hundreds of extrusion events during a single continuous pass through this zone–followed by a physically decoupled observation zone. This design enables clear separation of fatigue induction from biomechanical evaluation. Our findings show that cyclic extrusion drives a progressive morphological transition in the RBC population from discocytes to echinocytes and spherocytes, along with reduced cell volume and surface area, increased membrane shear modulus, and elevated sphericity. Combined experiments and simulations reveal that the passage of spherocytes depends not only on their deformability but also critically on the relative size of the cells versus the channel dimensions. Furthermore, simulations of splenic filtration identify the sphericity index–not membrane stiffness–as the primary geometric factor governing RBC retention in IES. This work presents a high-throughput, label-free platform that disentangles RBC fatigue induction from post-fatigue analysis. It provides mechanistic insights into how repetitive mechanical stress regulates RBC aging and clearance, offering a valuable tool for advancing our understanding of RBC physiology in health and disease.

在其120天的循环周期中,红细胞(rbc)在穿过微血管和脾内皮间缝(IES)时经历了反复的机械变形。这种循环的机械负荷逐渐损害红细胞的变形能力,最终导致其被脾脏清除。然而,目前用于研究RBC疲劳的平台通常将机械加载与实时观察相结合,这模糊了循环应变的累积影响。为了解决这一限制,我们开发了一种集成的微流控芯片,配备了专用的“S”形疲劳区——每个红细胞在一次连续通过该区域时经历数百次挤压事件——随后是物理解耦的观察区。这种设计使疲劳诱导与生物力学评估清晰分离。我们的研究结果表明,循环挤压推动红细胞群体从椎间盘细胞向棘细胞和球细胞的渐进形态转变,同时细胞体积和表面积减少,膜剪切模量增加,球形度升高。综合实验和模拟表明,球细胞的通过不仅取决于它们的可变形性,而且关键地取决于细胞相对于通道尺寸的相对大小。此外,脾滤过的模拟表明球形指数——而不是膜刚度——是控制IES中红细胞保留的主要几何因素。这项工作提出了一个高通量,无标签的平台,从疲劳后分析中解开红细胞疲劳诱导。它提供了重复机械应力如何调节红细胞老化和清除的机制见解,为促进我们对健康和疾病中的红细胞生理学的理解提供了有价值的工具。
{"title":"High-throughput biomimetic cycling of red blood cells: elucidating the morpho-mechanical determinants of fatigue and clearance","authors":"Yahui Du, Wenjiao Wu, Yuexiu Chen, Lihang Zhu, Shuhao Ma, Fengjiang Zhang and Xuejin Li","doi":"10.1039/D5LC01022E","DOIUrl":"10.1039/D5LC01022E","url":null,"abstract":"<p >During their 120-day circulatory lifespan, red blood cells (RBCs) undergo repeated mechanical deformation as they traverse microcapillaries and splenic inter-endothelial slits (IES). This cyclic mechanical loading gradually impairs RBC deformability, ultimately leading to their clearance by the spleen. However, current platforms for investigating RBC fatigue often couple mechanical loading with real-time observation, which obscures the cumulative impact of cyclic strain. To address this limitation, we developed an integrated microfluidic chip equipped with a dedicated “S”-shaped fatigue zone–each RBC experiences hundreds of extrusion events during a single continuous pass through this zone–followed by a physically decoupled observation zone. This design enables clear separation of fatigue induction from biomechanical evaluation. Our findings show that cyclic extrusion drives a progressive morphological transition in the RBC population from discocytes to echinocytes and spherocytes, along with reduced cell volume and surface area, increased membrane shear modulus, and elevated sphericity. Combined experiments and simulations reveal that the passage of spherocytes depends not only on their deformability but also critically on the relative size of the cells <em>versus</em> the channel dimensions. Furthermore, simulations of splenic filtration identify the sphericity index–not membrane stiffness–as the primary geometric factor governing RBC retention in IES. This work presents a high-throughput, label-free platform that disentangles RBC fatigue induction from post-fatigue analysis. It provides mechanistic insights into how repetitive mechanical stress regulates RBC aging and clearance, offering a valuable tool for advancing our understanding of RBC physiology in health and disease.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 3","pages":" 551-563"},"PeriodicalIF":5.4,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145907979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fully printed and flexible patch for real-time wireless monitoring of the sweating rate with physiological detection 全印刷和柔性贴片,实时无线监测出汗率与生理检测。
IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-01-03 DOI: 10.1039/D5LC00755K
Hao Wen, Kailong Dong, Feiyang Huang, Zhiqing Gao, Zijian An, Rujing Sun, Xin Li, Qing Ye and Qingjun Liu

The human sweating rate reflects body hydration status and holds intrinsic significance for monitoring physiological health. This work presents a fully printed sweat rate sensor architecture, where the internal sensing layer is fabricated via aerosol jet printing at micrometer resolution to detect nanoliter-scale sweat volume changes in microfluidic channels. The sensor transduces internal microstructural variations into radiofrequency (RF) signals through energy coupling, enabling wireless transmission to external terminals. Leveraging the RF sensor's wireless compatibility, a pulse wave sensor for monitoring physiological changes is integrated into the system. This allows simultaneous operation with the sweat rate sensor without wired connections, ultimately forming a wireless and battery-free wearable patch suitable for detecting the skin sweating rate and heart rate during human activities. By analyzing the patch's wireless signals and extracting parameters including resonant frequency and amplitude, we develop a dual-mode sensing patch. The system evaluates the effects of daily activities like resting, walking, exercising and environmental factors like temperature on skin perspiration and heart rate. In addition, the fully printed technology adopted in this work provides ideas for the lightweight and low-cost development of wearable sweat sensing systems.

人体出汗率反映了人体的水合状态,对监测生理健康具有内在意义。这项工作提出了一个完全打印的汗液速率传感器架构,其中内部传感层是通过微米分辨率的气溶胶喷射打印制造的,以检测微流体通道中纳米级汗液体积的变化。传感器通过能量耦合将内部微结构变化转换为射频(RF)信号,从而实现无线传输到外部终端。利用射频传感器的无线兼容性,用于监测生理变化的脉冲波传感器集成到系统中。这样可以在没有有线连接的情况下与汗液传感器同时操作,最终形成一个无线和无电池的可穿戴贴片,适合检测人体活动时的皮肤出汗率和心率。通过对贴片的无线信号进行分析,提取谐振频率和幅值等参数,研制出双模传感贴片。该系统评估日常活动(如休息、散步、锻炼)和环境因素(如温度)对皮肤出汗和心率的影响。此外,本工作采用的全印刷技术为可穿戴式汗液传感系统的轻量化和低成本开发提供了思路。
{"title":"Fully printed and flexible patch for real-time wireless monitoring of the sweating rate with physiological detection","authors":"Hao Wen, Kailong Dong, Feiyang Huang, Zhiqing Gao, Zijian An, Rujing Sun, Xin Li, Qing Ye and Qingjun Liu","doi":"10.1039/D5LC00755K","DOIUrl":"10.1039/D5LC00755K","url":null,"abstract":"<p >The human sweating rate reflects body hydration status and holds intrinsic significance for monitoring physiological health. This work presents a fully printed sweat rate sensor architecture, where the internal sensing layer is fabricated <em>via</em> aerosol jet printing at micrometer resolution to detect nanoliter-scale sweat volume changes in microfluidic channels. The sensor transduces internal microstructural variations into radiofrequency (RF) signals through energy coupling, enabling wireless transmission to external terminals. Leveraging the RF sensor's wireless compatibility, a pulse wave sensor for monitoring physiological changes is integrated into the system. This allows simultaneous operation with the sweat rate sensor without wired connections, ultimately forming a wireless and battery-free wearable patch suitable for detecting the skin sweating rate and heart rate during human activities. By analyzing the patch's wireless signals and extracting parameters including resonant frequency and amplitude, we develop a dual-mode sensing patch. The system evaluates the effects of daily activities like resting, walking, exercising and environmental factors like temperature on skin perspiration and heart rate. In addition, the fully printed technology adopted in this work provides ideas for the lightweight and low-cost development of wearable sweat sensing systems.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 3","pages":" 618-626"},"PeriodicalIF":5.4,"publicationDate":"2026-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145907811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CombiCTx: screening diffusion gradients of anti-cancer drug combinations CombiCTx:筛选抗癌药物组合的扩散梯度
IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-01-01 DOI: 10.1039/D5LC00686D
Christina Stelzl, Ada Lerma-Clavero, Selina Camenisch, Benoit Simon, Olle Eriksson, Oliver Degerstedt, Hans Lennernäs, Femke Heindryckx, Johan Kreuger and Paul O'Callaghan

The reduced effectiveness of chemotherapy in many patients undergoing treatment highlights the need for novel drug combinations that target drug resistance mechanisms contributing to tumor survival. Dynamic conditions within the tumor microenvironment influence the response to anti-cancer drugs. Accordingly, identifying effective drug concentrations and interactions (additive, synergistic, or antagonistic) in relevant tumor tissue models will inform new treatment combinations. To address this need for combinatorial chemotherapeutic (CTx) screening assays, we have developed a new assay called CombiCTx, which uses a device with three reservoirs containing gels loaded with anti-cancer drugs. The drug-loaded device is inverted and placed in a standard culture dish above cancer cells, and both are then enclosed in gel. Drugs diffuse from the reservoirs and expose cancer cells to overlapping dynamic drug gradients. We imaged diffusion of the anti-cancer drug doxorubicin in the assay using time-lapse microscopy, and established an imaging protocol for quantifying MDA-MB-231 breast cancer cell survival responses along drug gradients. Finally, evaluating combination effects of navitoclax and gemcitabine with CombiCTx revealed localized effects of navitoclax, attributed to limited diffusion, while gemcitabine seemed to diffuse readily throughout the assay and revealed a mild synergy in navitoclax affected regions. These data demonstrate the capacity of CombiCTx to evaluate the cytotoxic effects of anti-cancer drug combinations while accounting for drug diffusion differences, which is relevant in the context of the 3D tumor environment and may thereby help inform clinical treatment strategies.

化疗在许多患者中的有效性降低,这突出表明需要针对有助于肿瘤生存的耐药机制的新型药物组合。肿瘤微环境的动态条件影响肿瘤对抗癌药物的反应。因此,确定相关肿瘤组织模型中的有效药物浓度和相互作用(加性、增效或拮抗)将为新的治疗组合提供信息。为了满足这种对组合化疗(CTx)筛选测定的需求,我们开发了一种名为CombiCTx的新测定方法,该方法使用一个带有三个储层的装置,储层含有装载抗癌药物的凝胶。将装载药物的装置倒置并置于癌细胞上方的标准培养皿中,然后将两者都包裹在凝胶中。药物从储存库中扩散,使癌细胞暴露于重叠的动态药物梯度中。我们在实验中使用延时显微镜对抗癌药物阿霉素的扩散进行成像,并建立了一种成像方案,用于定量MDA-MB-231乳腺癌细胞沿药物梯度的生存反应。最后,在CombiCTx中评估navitoclax和吉西他滨的联合效应,发现navitoclax的作用是有效的,但局部的作用,归因于有限的扩散,而吉西他滨似乎在整个试验中很容易扩散,并在navitoclax区域显示出轻微的协同作用。这些数据证明了CombiCTx在考虑药物扩散差异的同时评估抗癌药物组合的细胞毒性作用的能力,这与3D肿瘤环境相关,因此可能有助于为临床治疗策略提供信息。
{"title":"CombiCTx: screening diffusion gradients of anti-cancer drug combinations","authors":"Christina Stelzl, Ada Lerma-Clavero, Selina Camenisch, Benoit Simon, Olle Eriksson, Oliver Degerstedt, Hans Lennernäs, Femke Heindryckx, Johan Kreuger and Paul O'Callaghan","doi":"10.1039/D5LC00686D","DOIUrl":"10.1039/D5LC00686D","url":null,"abstract":"<p >The reduced effectiveness of chemotherapy in many patients undergoing treatment highlights the need for novel drug combinations that target drug resistance mechanisms contributing to tumor survival. Dynamic conditions within the tumor microenvironment influence the response to anti-cancer drugs. Accordingly, identifying effective drug concentrations and interactions (additive, synergistic, or antagonistic) in relevant tumor tissue models will inform new treatment combinations. To address this need for combinatorial chemotherapeutic (CTx) screening assays, we have developed a new assay called CombiCTx, which uses a device with three reservoirs containing gels loaded with anti-cancer drugs. The drug-loaded device is inverted and placed in a standard culture dish above cancer cells, and both are then enclosed in gel. Drugs diffuse from the reservoirs and expose cancer cells to overlapping dynamic drug gradients. We imaged diffusion of the anti-cancer drug doxorubicin in the assay using time-lapse microscopy, and established an imaging protocol for quantifying MDA-MB-231 breast cancer cell survival responses along drug gradients. Finally, evaluating combination effects of navitoclax and gemcitabine with CombiCTx revealed localized effects of navitoclax, attributed to limited diffusion, while gemcitabine seemed to diffuse readily throughout the assay and revealed a mild synergy in navitoclax affected regions. These data demonstrate the capacity of CombiCTx to evaluate the cytotoxic effects of anti-cancer drug combinations while accounting for drug diffusion differences, which is relevant in the context of the 3D tumor environment and may thereby help inform clinical treatment strategies.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 3","pages":" 695-710"},"PeriodicalIF":5.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/lc/d5lc00686d?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145895372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integrated high-performance microcapsule fire extinguishing system for confined spaces with real-time monitoring and early warning capabilities 集成高性能微胶囊灭火系统,用于密闭空间,具有实时监控和预警能力
IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2025-12-30 DOI: 10.1039/D5LC01053E
Qiaosheng Pan, Jiachao Zhang, Jijie Fu, Ning Sang, Dang Ding, Peng Zhang, Chen Li, Tianpei Zhou, Ting Si, Fangsheng Huang and Zhiqiang Zhu

Small-scale fires in confined spaces represent critical precursors to catastrophic disasters, making their early suppression essential for safeguarding lives and property. However, at present, fire extinguishing systems used in confined space still suffer from several limitations, such as large physical scale, the absence of real-time temperature monitoring, delayed release of the extinguishing agent and early efficient fire extinguishing ability. These shortcomings seriously hinder the timely prevention of early fire and restrict the choice of appropriate post-fire management strategies. To overcome these challenges, we have developed an integrated fire extinguishing system based on high-performance composite microcapsules. This miniaturized system integrates real-time wireless monitoring, early warning, rapid fire extinguishing and cooling capacity at the initial stage of fire. The system consists of three core components: a mounting assembly supporting flexible installation, a fire suppression module composed of two-dimensional microcapsule patches, and a temperature-sensing unit for continuous environmental monitoring. The microcapsule patches, thermally triggered to release fire-extinguishing agents, exhibit high extinguishing efficiency and rapid cooling, thereby enabling proactive fire containment in confined spaces. The sensing module provides real-time thermal surveillance with wireless data transmission to remote terminals. Importantly, the temperature monitoring and early-warning system operates independently of the extinguishing agent release to ensure there is no delay in suppression. Experimental validation confirms the system's efficacy in rapid fire suppression, ambient cooling, and intelligent early warning, offering an innovative solution for confined space fire risk mitigation.

密闭空间内的小规模火灾是灾难性灾害的重要先兆,因此及早扑灭火灾对于保护生命和财产至关重要。然而,传统的密闭空间灭火系统往往缺乏实时温度监测和有效的主动灭火能力,再加上人为因素,如注意力的疏忽,经常导致火灾升级。为了解决这些限制,我们开发了一种基于高性能复合微胶囊的综合灭火系统,能够在初期阶段快速灭火和环境冷却。该系统结合了实时热监测、无线数据传输和智能温度调节。该系统由三个核心部件组成:一个支持灵活安装的安装组件,一个由二维微胶囊贴片组成的灭火模块,以及一个用于连续环境监测的温度传感单元。微胶囊贴片,热触发释放灭火剂,具有高灭火效率和快速冷却,从而实现密闭空间的主动灭火。同时,传感模块提供实时热监控,无线数据传输到远程终端。实验验证证实了该系统在快速灭火、环境冷却和智能预警方面的有效性,为降低密闭空间火灾风险提供了一种创新的解决方案。
{"title":"Integrated high-performance microcapsule fire extinguishing system for confined spaces with real-time monitoring and early warning capabilities","authors":"Qiaosheng Pan, Jiachao Zhang, Jijie Fu, Ning Sang, Dang Ding, Peng Zhang, Chen Li, Tianpei Zhou, Ting Si, Fangsheng Huang and Zhiqiang Zhu","doi":"10.1039/D5LC01053E","DOIUrl":"10.1039/D5LC01053E","url":null,"abstract":"<p >Small-scale fires in confined spaces represent critical precursors to catastrophic disasters, making their early suppression essential for safeguarding lives and property. However, at present, fire extinguishing systems used in confined space still suffer from several limitations, such as large physical scale, the absence of real-time temperature monitoring, delayed release of the extinguishing agent and early efficient fire extinguishing ability. These shortcomings seriously hinder the timely prevention of early fire and restrict the choice of appropriate post-fire management strategies. To overcome these challenges, we have developed an integrated fire extinguishing system based on high-performance composite microcapsules. This miniaturized system integrates real-time wireless monitoring, early warning, rapid fire extinguishing and cooling capacity at the initial stage of fire. The system consists of three core components: a mounting assembly supporting flexible installation, a fire suppression module composed of two-dimensional microcapsule patches, and a temperature-sensing unit for continuous environmental monitoring. The microcapsule patches, thermally triggered to release fire-extinguishing agents, exhibit high extinguishing efficiency and rapid cooling, thereby enabling proactive fire containment in confined spaces. The sensing module provides real-time thermal surveillance with wireless data transmission to remote terminals. Importantly, the temperature monitoring and early-warning system operates independently of the extinguishing agent release to ensure there is no delay in suppression. Experimental validation confirms the system's efficacy in rapid fire suppression, ambient cooling, and intelligent early warning, offering an innovative solution for confined space fire risk mitigation.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 3","pages":" 576-590"},"PeriodicalIF":5.4,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145895373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gravity-induced tunable asymmetric droplet splitting for flexible and precise reagent formulation on vertical digital microfluidic devices 重力诱导的可调不对称液滴分裂在垂直数字微流体中用于灵活和精确的试剂配制。
IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2025-12-29 DOI: 10.1039/D5LC00868A
Juyue Dong, Zerui Song, Kunlun Guo, Hang Xu, Zhibei Qu, Zhen Gu and Huifeng Wang

Digital microfluidic devices enable parallel, quantitative and flexible handling of discrete droplets via electrowetting on dielectric (EWOD) force. However, droplet splitting behavior in conventional digital microfluidic devices is limited by the geometry of actuating electrodes. In this study, we proposed a gravity-induced size-tuning splitting (GITS) method, which has no requirements for specialized electrodes or complicated chip configurations. Both experimental and simulation results demonstrated that gravity facilitates the droplet generation by directionally enhancing the EWOD force in a vertical digital microfluidic chip. Further observation revealed that the size tunability was affected by the droplet volume, voltage amplitude, and especially contact line ratios between droplet and electrodes. Moreover, to achieve reliable on-chip operations, the critical size of the droplet for passive dropping was investigated, which exhibited a functional relationship with the gap height. Then GITS was implemented by integrating an artificial intelligence (AI)-driven feedback control of the contact line and the gravity induced droplet dropping. As a result, it achieved wide splitting ratios from 1 to 7.33, with the coefficient of variation below 3%. Finally, GITS was applied to manage reagents of various sizes for on-chip cell viability assays, demonstrating its potential for flexible reagent configuration in future biomedical applications.

数字微流体能够通过电介质上的电润湿(EWOD)力对离散液滴进行并行,定量和灵活的处理。然而,在传统的数字微流体中,液滴的分裂行为受到致动电极几何形状的限制。在这项研究中,我们提出了一种重力诱导的尺寸调谐分裂方法(GITS),该方法不需要专门的电极或复杂的芯片结构。实验和仿真结果均表明,重力通过定向增强垂直数字微流控芯片内的EWOD力来促进液滴的生成。进一步观察发现,液滴体积、电压幅值、特别是液滴与电极之间的接触线比对尺寸的可调性有影响。此外,为了实现可靠的片上操作,研究了被动滴注的临界液滴尺寸,其与间隙高度呈函数关系。然后,将人工智能驱动的接触线反馈控制与重力诱导滴下相结合,实现了GITS。在1 ~ 7.33范围内实现了较宽的劈裂比,变异系数在3%以下。最后,GITS被应用于管理不同尺寸的芯片上细胞活力测定试剂,展示了其在未来生物医学应用中灵活试剂配置的潜力。
{"title":"Gravity-induced tunable asymmetric droplet splitting for flexible and precise reagent formulation on vertical digital microfluidic devices","authors":"Juyue Dong, Zerui Song, Kunlun Guo, Hang Xu, Zhibei Qu, Zhen Gu and Huifeng Wang","doi":"10.1039/D5LC00868A","DOIUrl":"10.1039/D5LC00868A","url":null,"abstract":"<p >Digital microfluidic devices enable parallel, quantitative and flexible handling of discrete droplets <em>via</em> electrowetting on dielectric (EWOD) force. However, droplet splitting behavior in conventional digital microfluidic devices is limited by the geometry of actuating electrodes. In this study, we proposed a gravity-induced size-tuning splitting (GITS) method, which has no requirements for specialized electrodes or complicated chip configurations. Both experimental and simulation results demonstrated that gravity facilitates the droplet generation by directionally enhancing the EWOD force in a vertical digital microfluidic chip. Further observation revealed that the size tunability was affected by the droplet volume, voltage amplitude, and especially contact line ratios between droplet and electrodes. Moreover, to achieve reliable on-chip operations, the critical size of the droplet for passive dropping was investigated, which exhibited a functional relationship with the gap height. Then GITS was implemented by integrating an artificial intelligence (AI)-driven feedback control of the contact line and the gravity induced droplet dropping. As a result, it achieved wide splitting ratios from 1 to 7.33, with the coefficient of variation below 3%. Finally, GITS was applied to manage reagents of various sizes for on-chip cell viability assays, demonstrating its potential for flexible reagent configuration in future biomedical applications.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 3","pages":" 725-734"},"PeriodicalIF":5.4,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145847383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A mechanomimetic model of skin fibrosis 皮肤纤维化的机械模拟模型。
IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2025-12-29 DOI: 10.1039/D5LC00560D
Alberto Pappalardo, Deniz Ornek, Laura Garriga Cerda, Charlotte Y. Lee, Kristin Myers, Jeffrey W. Kysar and Hasan Erbil Abaci

Skin fibrosis results from excessive extracellular matrix (ECM) deposition and tissue remodeling due to persistent inflammation and mechanotransduction dysregulation. Current in vivo animal models lack human relevance, while conventional 2D and 3D in vitro models misrepresent physiological mechanical forces. To address this gap, we developed a miniaturized edgeless-skin chip (ESC) platform with gravity-driven perfusion, enabling enhanced biomechanical mimicry for fibrosis modeling. ESCs present bioengineered skin grown around a 3D-printed scaffold, mimicking the continuous geometry of human skin and in vivo mechanical balance. Compared to conventional skin constructs (CSCs) that have open boundaries on all sides, ESCs exhibited higher sensitivity to TGF-β1, leading to increased ECM deposition, myofibroblast activation, YAP signaling upregulation, matrix stiffness and reduced hydraulic permeability. Inhibiting YAP signaling with verteporfin (VTP) reduced collagen deposition, prevented tissue stiffening, and attenuated several fibrosis markers, confirming the role of mechanotransduction in fibrosis progression using human cells. Transcriptome analysis revealed upregulation of fibrosis-associated genes, including COL10A1, COL11A1, and ACTA2, counterbalanced by elevation of anti-fibrotic regulators such as DKK2, which suggests the activation of negative feedback mechanisms. These findings establish the ESC platform as a robust human-relevant mechanomimetic model for studying fibrosis and evaluating anti-fibrotic therapies, addressing a critical need for translational drug discovery.

皮肤纤维化是由过度的细胞外基质(ECM)沉积和持续炎症和机械转导失调引起的组织重塑引起的。目前的体内动物模型缺乏与人类的相关性,而传统的2D和3D体外模型错误地描述了生理机械力。为了解决这一问题,我们开发了一种小型化的无边缘皮肤芯片(ESC)平台,该平台具有重力驱动的灌注,可以增强纤维化建模的生物力学模拟。ESCs是围绕3d打印支架生长的生物工程皮肤,模仿人类皮肤的连续几何形状和体内机械平衡。与四面开放边界的传统皮肤构建体(CSCs)相比,ESCs对TGF-β1表现出更高的敏感性,导致ECM沉积增加,肌成纤维细胞活化,YAP信号上调,基质刚度和水力渗透性降低。用椎泊芬(VTP)抑制YAP信号可以减少胶原沉积,防止组织硬化,并减弱几种纤维化标志物,证实了机械转导在人类细胞纤维化进展中的作用。转录组分析显示,包括COL10A1、COL11A1和ACTA2在内的纤维化相关基因上调,被抗纤维化调节因子如DKK2的升高所抵消,这表明负反馈机制的激活。这些发现建立了ESC平台作为研究纤维化和评估抗纤维化治疗的强大的人类相关机械模拟模型,解决了转化药物发现的关键需求。
{"title":"A mechanomimetic model of skin fibrosis","authors":"Alberto Pappalardo, Deniz Ornek, Laura Garriga Cerda, Charlotte Y. Lee, Kristin Myers, Jeffrey W. Kysar and Hasan Erbil Abaci","doi":"10.1039/D5LC00560D","DOIUrl":"10.1039/D5LC00560D","url":null,"abstract":"<p >Skin fibrosis results from excessive extracellular matrix (ECM) deposition and tissue remodeling due to persistent inflammation and mechanotransduction dysregulation. Current <em>in vivo</em> animal models lack human relevance, while conventional 2D and 3D <em>in vitro</em> models misrepresent physiological mechanical forces. To address this gap, we developed a miniaturized edgeless-skin chip (ESC) platform with gravity-driven perfusion, enabling enhanced biomechanical mimicry for fibrosis modeling. ESCs present bioengineered skin grown around a 3D-printed scaffold, mimicking the continuous geometry of human skin and <em>in vivo</em> mechanical balance. Compared to conventional skin constructs (CSCs) that have open boundaries on all sides, ESCs exhibited higher sensitivity to TGF-β1, leading to increased ECM deposition, myofibroblast activation, YAP signaling upregulation, matrix stiffness and reduced hydraulic permeability. Inhibiting YAP signaling with verteporfin (VTP) reduced collagen deposition, prevented tissue stiffening, and attenuated several fibrosis markers, confirming the role of mechanotransduction in fibrosis progression using human cells. Transcriptome analysis revealed upregulation of fibrosis-associated genes, including COL10A1, COL11A1, and ACTA2, counterbalanced by elevation of anti-fibrotic regulators such as DKK2, which suggests the activation of negative feedback mechanisms. These findings establish the ESC platform as a robust human-relevant mechanomimetic model for studying fibrosis and evaluating anti-fibrotic therapies, addressing a critical need for translational drug discovery.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 3","pages":" 665-680"},"PeriodicalIF":5.4,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145950888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A comprehensive toolkit for manipulation and analysis of sprouting capillary networks based on magnetic ordering of multiple EC-coated microcarriers and their use in tissue modelling and drug testing. 一个全面的工具包,用于操作和分析发芽毛细血管网络,基于多个ec涂层微载体的磁排序及其在组织建模和药物测试中的应用。
IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2025-12-24 DOI: 10.1039/d5lc00664c
Katarzyna Olga Rojek, Antoni Wrzos, Fabio Maiullari, Konrad Gizynski, Maria Grazia Ceraolo, Claudia Bearzi, Roberto Rizzi, Piotr Szymczak, Jan Guzowski
Despite significant developments in endothelial-cell (EC) manipulation techniques, an in vitro model of a functional microvasculature with controlled local interconnectivity (< 1 mm length scale) under well-defined global architecture (~1 cm length scale) is still lacking. Here, we report the generation of such controlled multi-scale vascular networks via manipulation of tens of sprouting EC microcarriers. We exploit magnetic patterning to assemble superparamagnetic mi-crobeads coated with human umbilical vein endothelial cells (HUVECs) into ordered arrays and establish effective growth rules governing the directionality of sprouting and the development of interconnections between the neighboring beads depending on the applied bead-bead spacing. The microcarrier-based approach offers a range of advantages over conventional EC-manipulation techniques including: (i) expedited sprouting, (ii) spatial control over the intercon-nections, (iii) reduction in cell consumption by even >100x, and (iv) a native high-throughput format. We co-develop a multiparametric morphometric analysis tool and demonstrate high-content assessment of drug-induced vascular remodeling in 3D tumor microenvironments. Over-all, we propose a uniquely precise and standardized vascular tissue-engineering and imaging toolkit with applications, e.g., in angiogenesis/anastomosis research as well as high-throughput drug testing including personalized therapies.
尽管内皮细胞(EC)操作技术取得了重大进展,但在明确定义的全局结构(~1厘米长度尺度)下,具有控制局部互联性(1毫米长度尺度)的功能性微血管的体外模型仍然缺乏。在这里,我们报告了通过操纵数十个发芽的EC微载体来产生这种受控的多尺度维管网络。我们利用磁成像技术将涂有人脐静脉内皮细胞(HUVECs)的超顺磁性微珠组装成有序阵列,并建立有效的生长规则来控制发芽的方向性,以及根据应用的珠粒间距在相邻珠粒之间形成互连。与传统的ec操作技术相比,基于微载体的方法提供了一系列优势,包括:(i)加速发芽,(ii)对互连的空间控制,(iii)将细胞消耗减少100倍,以及(iv)原生高通量格式。我们共同开发了一种多参数形态计量分析工具,并展示了3D肿瘤微环境中药物诱导血管重构的高含量评估。总的来说,我们提出了一个独特的精确和标准化的血管组织工程和成像工具包,应用于血管生成/吻合研究以及包括个性化治疗在内的高通量药物测试。
{"title":"A comprehensive toolkit for manipulation and analysis of sprouting capillary networks based on magnetic ordering of multiple EC-coated microcarriers and their use in tissue modelling and drug testing.","authors":"Katarzyna Olga Rojek, Antoni Wrzos, Fabio Maiullari, Konrad Gizynski, Maria Grazia Ceraolo, Claudia Bearzi, Roberto Rizzi, Piotr Szymczak, Jan Guzowski","doi":"10.1039/d5lc00664c","DOIUrl":"https://doi.org/10.1039/d5lc00664c","url":null,"abstract":"Despite significant developments in endothelial-cell (EC) manipulation techniques, an in vitro model of a functional microvasculature with controlled local interconnectivity (&lt; 1 mm length scale) under well-defined global architecture (~1 cm length scale) is still lacking. Here, we report the generation of such controlled multi-scale vascular networks via manipulation of tens of sprouting EC microcarriers. We exploit magnetic patterning to assemble superparamagnetic mi-crobeads coated with human umbilical vein endothelial cells (HUVECs) into ordered arrays and establish effective growth rules governing the directionality of sprouting and the development of interconnections between the neighboring beads depending on the applied bead-bead spacing. The microcarrier-based approach offers a range of advantages over conventional EC-manipulation techniques including: (i) expedited sprouting, (ii) spatial control over the intercon-nections, (iii) reduction in cell consumption by even &gt;100x, and (iv) a native high-throughput format. We co-develop a multiparametric morphometric analysis tool and demonstrate high-content assessment of drug-induced vascular remodeling in 3D tumor microenvironments. Over-all, we propose a uniquely precise and standardized vascular tissue-engineering and imaging toolkit with applications, e.g., in angiogenesis/anastomosis research as well as high-throughput drug testing including personalized therapies.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"25 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145823780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Tumor Spheroid Array Chip for High-Fidelity Evaluation of Liposomal Drug Delivery Through the EPR Effect 一种肿瘤球体阵列芯片,用于通过EPR效应高保真评估脂质体给药
IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2025-12-23 DOI: 10.1039/d5lc00893j
Yedam Lee, Sujin Kim, Hyeyeon Koh, Jung Yeon Han, Jihoon Ko, Yeonwoo Park
Conventional two-dimensional (2D) culture systems fail to recapitulate the structural and functional complexity of the tumor microenvironment (TME), limiting their translational relevance for preclinical drug evaluation. Here, we present a high-throughput microfluidic Tumor Spheroid Array (TSA)-Chip for investigating nanocarrier-based cancer therapies under physiologically perfusable conditions. Multicellular colorectal tumor spheroids—comprising cancer cells, endothelial cells, and fibroblasts—were embedded in a fibrin hydrogel to facilitate the formation of peritumoral vascular networks. The TSA-Chip enables continuous medium perfusion, live imaging, and quantitative assessment of vascular permeability. Using this platform, we evaluated the delivery and therapeutic efficacy of liposomal 5-fluorouracil (5-FU), leveraging the enhanced permeability and retention (EPR) effect. Compared to free 5-FU, the liposomal formulation showed improved tumor-specific accumulation and reduced vascular leakage. Furthermore, combination treatment with the anti-angiogenic agent Cyramza™ (ramucirumab) enhanced tumor suppression while preserving vascular integrity. This scalable and physiologically relevant platform provides a robust preclinical model for assessing nanoparticle transport and therapeutic outcomes in a perfusable TME, advancing precision oncology research.
传统的二维(2D)培养系统无法概括肿瘤微环境(TME)的结构和功能复杂性,限制了它们在临床前药物评估中的翻译相关性。在这里,我们提出了一种高通量微流控肿瘤球体阵列(TSA)芯片,用于研究生理可灌注条件下基于纳米载体的癌症治疗。多细胞结直肠肿瘤球体——包括癌细胞、内皮细胞和成纤维细胞——被包裹在纤维蛋白水凝胶中,以促进肿瘤周围血管网络的形成。TSA-Chip可实现连续介质灌注、实时成像和血管通透性定量评估。利用这个平台,我们评估了5-氟尿嘧啶脂质体(5-FU)的递送和治疗效果,利用增强的渗透性和潴留(EPR)效应。与游离5-FU相比,脂质体制剂可改善肿瘤特异性积累,减少血管渗漏。此外,与抗血管生成剂Cyramza™(ramucirumab)联合治疗增强了肿瘤抑制,同时保持了血管完整性。这个可扩展的和生理学相关的平台提供了一个强大的临床前模型,用于评估纳米颗粒在可灌注TME中的转运和治疗结果,推进精准肿瘤学研究。
{"title":"A Tumor Spheroid Array Chip for High-Fidelity Evaluation of Liposomal Drug Delivery Through the EPR Effect","authors":"Yedam Lee, Sujin Kim, Hyeyeon Koh, Jung Yeon Han, Jihoon Ko, Yeonwoo Park","doi":"10.1039/d5lc00893j","DOIUrl":"https://doi.org/10.1039/d5lc00893j","url":null,"abstract":"Conventional two-dimensional (2D) culture systems fail to recapitulate the structural and functional complexity of the tumor microenvironment (TME), limiting their translational relevance for preclinical drug evaluation. Here, we present a high-throughput microfluidic Tumor Spheroid Array (TSA)-Chip for investigating nanocarrier-based cancer therapies under physiologically perfusable conditions. Multicellular colorectal tumor spheroids—comprising cancer cells, endothelial cells, and fibroblasts—were embedded in a fibrin hydrogel to facilitate the formation of peritumoral vascular networks. The TSA-Chip enables continuous medium perfusion, live imaging, and quantitative assessment of vascular permeability. Using this platform, we evaluated the delivery and therapeutic efficacy of liposomal 5-fluorouracil (5-FU), leveraging the enhanced permeability and retention (EPR) effect. Compared to free 5-FU, the liposomal formulation showed improved tumor-specific accumulation and reduced vascular leakage. Furthermore, combination treatment with the anti-angiogenic agent Cyramza™ (ramucirumab) enhanced tumor suppression while preserving vascular integrity. This scalable and physiologically relevant platform provides a robust preclinical model for assessing nanoparticle transport and therapeutic outcomes in a perfusable TME, advancing precision oncology research.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"26 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145813281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Magnetophoretic long jump of magnetic microparticles in an engineered magnetic stray field landscape for highly localized and large throughput on-chip fractionation 磁微粒在杂散磁场环境下的磁电泳远跳,用于高定域和大通量的片上分选。
IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Pub Date : 2025-12-23 DOI: 10.1039/D5LC01000D
Rico Huhnstock, Lukas Paetzold, Piotr Kuświk and Arno Ehresmann

A common issue faced by magnetic particle-based lab-on-a-chip systems, e.g., for medical diagnostics, is the intrinsic fabrication-related polydispersity in particle sizes and magnetic properties. Therefore, to reduce this variation, it is prudent to integrate a pre-separation procedure for the particles into the overall workflow of the system. In this work, a concept for the controlled on-chip fractionation of micron-sized superparamagnetic beads (SPBs) is introduced, which is applicable for sorting magnetic particles according to their properties in a continuous operation mode. A specifically designed magnetic domain pattern is imprinted into an exchange-biased thin film system to generate a tailored magnetic stray field landscape (MFL), enabling lateral transport of SPBs when superposing the MFL with external magnetic field pulses. The domain pattern consists of parallel stripes with gradually increasing and decreasing width, resulting in a step-wise jumping motion of SPBs with increasing/decreasing jump distance. SPBs with different magnetophoretic mobilities, determined, among others, by the particle size and magnetic susceptibility, discontinue their lateral motion at different jump distances, i.e., different lateral positions on the substrate. Thorough analysis of the motion using optical microscopy and particle tracking revealed that an increasing stripe width not only leads to a larger jump distance but also to a lowered jump velocity. As a consequence, particles are spatially separated according to their magnetic and structural properties with a large throughput and time efficiency, as simultaneous sorting occurs for all particles present on the substrate using a constant sequence of short external field pulses.

基于磁性颗粒的芯片实验室系统(例如用于医疗诊断)面临的一个共同问题是,颗粒大小和磁性的固有制造相关的多分散性。因此,为了减少这种变化,将颗粒的预分离程序集成到系统的整体工作流程中是谨慎的。本文提出了一种微米级超顺磁珠(SPBs)片上控制分选的概念,该概念适用于在连续操作模式下根据磁性颗粒的性质对其进行分选。一个特别设计的磁畴图案被印印到交换偏置薄膜系统中,以产生定制的磁杂散场景观(MFL),当MFL与外部磁场脉冲叠加时,可以实现spb的横向传输。畴图由宽度逐渐增大和减小的平行条纹组成,导致spb随跳跃距离的增大和减小呈阶梯跳跃运动。具有不同磁泳流动性的spb(其中由粒径和磁化率决定)在不同的跳跃距离,即在衬底上的不同横向位置停止其横向运动。利用光学显微镜和粒子跟踪对运动进行深入分析表明,增加条纹宽度不仅会导致更大的跳跃距离,而且会降低跳跃速度。因此,颗粒根据其磁性和结构特性进行空间分离,具有大通量和时间效率,因为使用恒定序列的短外场脉冲可以同时对衬底上的所有颗粒进行分选。
{"title":"Magnetophoretic long jump of magnetic microparticles in an engineered magnetic stray field landscape for highly localized and large throughput on-chip fractionation","authors":"Rico Huhnstock, Lukas Paetzold, Piotr Kuświk and Arno Ehresmann","doi":"10.1039/D5LC01000D","DOIUrl":"10.1039/D5LC01000D","url":null,"abstract":"<p >A common issue faced by magnetic particle-based lab-on-a-chip systems, <em>e.g.</em>, for medical diagnostics, is the intrinsic fabrication-related polydispersity in particle sizes and magnetic properties. Therefore, to reduce this variation, it is prudent to integrate a pre-separation procedure for the particles into the overall workflow of the system. In this work, a concept for the controlled on-chip fractionation of micron-sized superparamagnetic beads (SPBs) is introduced, which is applicable for sorting magnetic particles according to their properties in a continuous operation mode. A specifically designed magnetic domain pattern is imprinted into an exchange-biased thin film system to generate a tailored magnetic stray field landscape (MFL), enabling lateral transport of SPBs when superposing the MFL with external magnetic field pulses. The domain pattern consists of parallel stripes with gradually increasing and decreasing width, resulting in a step-wise jumping motion of SPBs with increasing/decreasing jump distance. SPBs with different magnetophoretic mobilities, determined, among others, by the particle size and magnetic susceptibility, discontinue their lateral motion at different jump distances, <em>i.e.</em>, different lateral positions on the substrate. Thorough analysis of the motion using optical microscopy and particle tracking revealed that an increasing stripe width not only leads to a larger jump distance but also to a lowered jump velocity. As a consequence, particles are spatially separated according to their magnetic and structural properties with a large throughput and time efficiency, as simultaneous sorting occurs for all particles present on the substrate using a constant sequence of short external field pulses.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" 2","pages":" 494-506"},"PeriodicalIF":5.4,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/lc/d5lc01000d?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145807844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Lab on a Chip
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1