Abstract. Albarido NA, Tabugo SR. 2024. High-throughput analysis using 16S rRNA gene of bacterial communities present in selected bivalves and gastropods species from Bayug Island, Iligan City, Philippines. Biodiversitas 25: 431-438. Seashells, which include bivalves and gastropods, have global recognition for their significant contributions to the economy, ecology, and medicine. They hold value as a food source and are highly regarded as effective biological indicators. The objective of this study is to identify the bacterial communities present in selected edible species of bivalves (Pinctada margaritifera Linnaeus, 1758 and Anadara granosa Linnaeus, 1758) and gastropods (Canarium urceus Linnaeus, 1758 and Conus stercusmuscarum Linnaeus, 1758), through high-throughput sequencing metabarcoding. Bacterial samples were collected via a swabbing technique on the surface and inside parts of selected mollusc species, which were then placed on sterilized seawater for DNA extraction. Genomic DNA was isolated from the samples, and the V3-V4 region of the 16S rRNA gene was amplified and sequenced using the Illumina MiSeq platform. Four amplicon libraries were generated, representing the two bivalve and two gastropod species in the study area. Data analysis was conducted using the Parallel Meta Suite software. Upon quality control and processing, 173,489 amplicon sequence variants (ASVs) were obtained. Within the bacterial community, the most abundant genera included Stenotrophomonas, Vibrio, Serratia, Photobacterium, and Shewanella. The assessment of alpha diversity, using the Shannon index, indicated a higher diversity in A. granosa. Furthermore, the analysis using the PICRUSt algorithm within the Parallel Meta Suite unveiled the involvement of specific bacteria found in the selected gastropod and bivalve species in various functions. These functions encompass protein production, xenobiotic metabolism, biodegradation, and other metabolism-related processes, supporting these organisms' ecological and physiological roles.
{"title":"High-throughput analysis using 16S rRNA gene of bacterial communities present in selected bivalves and gastropods species from Bayug Island, Iligan City, Philippines","authors":"Nitcel Aymie Albarido, S. Tabugo","doi":"10.13057/biodiv/d250150","DOIUrl":"https://doi.org/10.13057/biodiv/d250150","url":null,"abstract":"Abstract. Albarido NA, Tabugo SR. 2024. High-throughput analysis using 16S rRNA gene of bacterial communities present in selected bivalves and gastropods species from Bayug Island, Iligan City, Philippines. Biodiversitas 25: 431-438. Seashells, which include bivalves and gastropods, have global recognition for their significant contributions to the economy, ecology, and medicine. They hold value as a food source and are highly regarded as effective biological indicators. The objective of this study is to identify the bacterial communities present in selected edible species of bivalves (Pinctada margaritifera Linnaeus, 1758 and Anadara granosa Linnaeus, 1758) and gastropods (Canarium urceus Linnaeus, 1758 and Conus stercusmuscarum Linnaeus, 1758), through high-throughput sequencing metabarcoding. Bacterial samples were collected via a swabbing technique on the surface and inside parts of selected mollusc species, which were then placed on sterilized seawater for DNA extraction. Genomic DNA was isolated from the samples, and the V3-V4 region of the 16S rRNA gene was amplified and sequenced using the Illumina MiSeq platform. Four amplicon libraries were generated, representing the two bivalve and two gastropod species in the study area. Data analysis was conducted using the Parallel Meta Suite software. Upon quality control and processing, 173,489 amplicon sequence variants (ASVs) were obtained. Within the bacterial community, the most abundant genera included Stenotrophomonas, Vibrio, Serratia, Photobacterium, and Shewanella. The assessment of alpha diversity, using the Shannon index, indicated a higher diversity in A. granosa. Furthermore, the analysis using the PICRUSt algorithm within the Parallel Meta Suite unveiled the involvement of specific bacteria found in the selected gastropod and bivalve species in various functions. These functions encompass protein production, xenobiotic metabolism, biodegradation, and other metabolism-related processes, supporting these organisms' ecological and physiological roles.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"22 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140457713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Novita Permata Putri, Muhammad Rifat Muharam, Abdulrahman Ritonga, Corina Dewi Ruswanti, Dian Arthen, Munasik Munasik
Abstract. Putri NP, Muharam MR, Ritonga A, Ruswanti CD, Arthen D, Munasik. 2024. Examining the effectiveness of bored pile coral for coral rehabilitation. Biodiversitas 25: 153-161. Semut Island is located in Kiabu Village, Anambas Islands, Riau, Indonesia, and has suffered significant damage to its coral cover due to blast fishing. To address this issue, Anambas Foundation initiated a coral reef rehabilitation on Semut Island using a customized artificial reef called Bored Pile Coral (BPC), a cylindrical structure made of concrete hammered into the seabed. This research aims to examine the effectiveness of BPC by monitoring its performance. Therefore, from February 2022 to January 2023, 1,500 BPCs were deployed and attached with corals covering an area of 170 m2. We quantified five ecological indicators, i.e., coral cover, survival rate, recruitment, growth rates, and fish biomass. Results showed an increase in coral cover from 3.8% to 8%. The survival rate was 64%, and the mean monthly growth rates varied among three genera: Pocillopora sp. (0.235±0.02 month-1), Acropora sp. (0.22±0.03 month-1) and Porites sp. (0.08±0.02 month-1). The monthly mean fish biomass data showed the biomass was 427,25 Kg/Ha and increased 3,9 times since the beginning of the research. BPC's structure has been proven to act as stable media for hard corals, although the modification may be necessary to enhance structural complexity and suitability for carnivorous fish habitats.
{"title":"Examining the effectiveness of bored pile coral for coral rehabilitation","authors":"Novita Permata Putri, Muhammad Rifat Muharam, Abdulrahman Ritonga, Corina Dewi Ruswanti, Dian Arthen, Munasik Munasik","doi":"10.13057/biodiv/d250117","DOIUrl":"https://doi.org/10.13057/biodiv/d250117","url":null,"abstract":"Abstract. Putri NP, Muharam MR, Ritonga A, Ruswanti CD, Arthen D, Munasik. 2024. Examining the effectiveness of bored pile coral for coral rehabilitation. Biodiversitas 25: 153-161. Semut Island is located in Kiabu Village, Anambas Islands, Riau, Indonesia, and has suffered significant damage to its coral cover due to blast fishing. To address this issue, Anambas Foundation initiated a coral reef rehabilitation on Semut Island using a customized artificial reef called Bored Pile Coral (BPC), a cylindrical structure made of concrete hammered into the seabed. This research aims to examine the effectiveness of BPC by monitoring its performance. Therefore, from February 2022 to January 2023, 1,500 BPCs were deployed and attached with corals covering an area of 170 m2. We quantified five ecological indicators, i.e., coral cover, survival rate, recruitment, growth rates, and fish biomass. Results showed an increase in coral cover from 3.8% to 8%. The survival rate was 64%, and the mean monthly growth rates varied among three genera: Pocillopora sp. (0.235±0.02 month-1), Acropora sp. (0.22±0.03 month-1) and Porites sp. (0.08±0.02 month-1). The monthly mean fish biomass data showed the biomass was 427,25 Kg/Ha and increased 3,9 times since the beginning of the research. BPC's structure has been proven to act as stable media for hard corals, although the modification may be necessary to enhance structural complexity and suitability for carnivorous fish habitats.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"307 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140457537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract. Manoppo JSS, Putra RE, Dungani R, Ahmad I. 2024. The diversity and distribution of residential termites in the Minahasa Raya Region, North Sulawesi Province, Indonesia. Biodiversitas 25: 39-48. Termites are major pests in urban settings, particularly due to their preference for wood, despite its ecological importance leading to structural damage to buildings. Studies showed a gap in the North Sulawesi Region, Indonesia, particularly in termite distribution, control, and economic impact in residential zones. This study aimed to (i) gather preliminary data on termite species in Minahasa and North Sulawesi from the literature; (ii) locate termite colonies via house/building surveys and baiting; (iii) map the distribution of identified termite species by Geographical Information System (GIS); and (iv) record environmental characteristics relevant to termite distribution and related damages. The study revealed a diversity of residential termite species in terms of morphological characteristics and habitats. Three termite families were identified: Rhinotermitidae, Termitidae, and Kalotermitidae, encompassing five sub-families and six genera, with the most notable species being Cryptotermes brevis Walker 1853, Odontotermes javanicus Holmgren, 1912, and Nasutitermes atripennis Haviland, 1898 among others. The Cryptotermes and Odontotermes were the most commonly encountered genera in Minahasa Raya's residential areas.
Abstract.Manoppo JSS, Putra RE, Dungani R, Ahmad I. 2024.印度尼西亚北苏拉威西省 Minahasa Raya 地区住宅白蚁的多样性和分布。Biodiversitas 25: 39-48.白蚁是城市环境中的主要害虫，特别是由于它们对木材的偏爱，尽管木材在生态方面的重要性导致了建筑物结构的破坏。研究显示，印度尼西亚北苏拉威西地区在白蚁分布、控制和对居民区的经济影响方面存在空白。本研究的目的是：(i) 从文献中收集有关米纳哈萨和北苏拉威西地区白蚁种类的初步数据；(ii) 通过房屋/建筑物调查和诱饵定位白蚁群落；(iii) 利用地理信息系统（GIS）绘制已识别白蚁种类的分布图；以及 (iv) 记录与白蚁分布和相关损害有关的环境特征。研究显示，住宅白蚁的形态特征和栖息地多种多样。确定了三个白蚁科：最著名的白蚁种类有：Cryptotermes brevis Walker 1853、Odontotermes javanicus Holmgren, 1912 和 Nasutitermes atripennis Haviland, 1898 等。在 Minahasa Raya 的居民区，最常遇到的虫属是隐翅虫和 Odontotermes。
{"title":"The diversity and distribution of residential termites in the Minahasa Raya Region, North Sulawesi Province, Indonesia","authors":"Jacklin Stella Salome Manoppo, Ramadhani Eka Putra, Rudi Dungani, Intan Ahmad","doi":"10.13057/biodiv/d250105","DOIUrl":"https://doi.org/10.13057/biodiv/d250105","url":null,"abstract":"Abstract. Manoppo JSS, Putra RE, Dungani R, Ahmad I. 2024. The diversity and distribution of residential termites in the Minahasa Raya Region, North Sulawesi Province, Indonesia. Biodiversitas 25: 39-48. Termites are major pests in urban settings, particularly due to their preference for wood, despite its ecological importance leading to structural damage to buildings. Studies showed a gap in the North Sulawesi Region, Indonesia, particularly in termite distribution, control, and economic impact in residential zones. This study aimed to (i) gather preliminary data on termite species in Minahasa and North Sulawesi from the literature; (ii) locate termite colonies via house/building surveys and baiting; (iii) map the distribution of identified termite species by Geographical Information System (GIS); and (iv) record environmental characteristics relevant to termite distribution and related damages. The study revealed a diversity of residential termite species in terms of morphological characteristics and habitats. Three termite families were identified: Rhinotermitidae, Termitidae, and Kalotermitidae, encompassing five sub-families and six genera, with the most notable species being Cryptotermes brevis Walker 1853, Odontotermes javanicus Holmgren, 1912, and Nasutitermes atripennis Haviland, 1898 among others. The Cryptotermes and Odontotermes were the most commonly encountered genera in Minahasa Raya's residential areas.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"384 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140457817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract. Keray AS, Widiati R, Kusumastuti TA. 2024. The role of local pigs for economic and socio-cultural in East Flores District, East Nusa Tenggara, Indonesia. Biodiversitas 25: 214-222. This study was conducted to identify the cultural activities related to swine rearing in East Flores District and analyze the decision making in choosing the types of rearing practice. Based on the survey using questionnaires from 120 pig farmers, which was conducted purposive, were those who had at least three sows for breeding, fattening with five heads of pigs, and combined with sows and sires. To identify the role of local pigs, as many as 3 informants consist of traditional leaders, farmers, and Agriculture Extension Officers, and analyzed by multinomial logistic regression model. The results showed that the traditional rituals that required the sacrifice of pigs included the ceremonies marking the start of building residential and traditional community buildings and dowry (belis) presentation in a wedding ceremony. The decision-making in choosing the rearing practice was influenced by pig ownership, care system (P<0.01), and income (P<0.05). Farmers who have small scale will choose extensive and fattening systems to reduce feed costs. Those practicing breeding are still maintaining local breeds because traditional rituals require it. Further efforts are needed to increase the populations of local breeds as well as cross-bred pigs to maintain the ceremonies in traditional rituals and income generation.
{"title":"The role of local pigs for economic and socio-cultural in East Flores District, East Nusa Tenggara, Indonesia","authors":"A. S. Keray, R. Widiati, T. A. Kusumastuti","doi":"10.13057/biodiv/d250124","DOIUrl":"https://doi.org/10.13057/biodiv/d250124","url":null,"abstract":"Abstract. Keray AS, Widiati R, Kusumastuti TA. 2024. The role of local pigs for economic and socio-cultural in East Flores District, East Nusa Tenggara, Indonesia. Biodiversitas 25: 214-222. This study was conducted to identify the cultural activities related to swine rearing in East Flores District and analyze the decision making in choosing the types of rearing practice. Based on the survey using questionnaires from 120 pig farmers, which was conducted purposive, were those who had at least three sows for breeding, fattening with five heads of pigs, and combined with sows and sires. To identify the role of local pigs, as many as 3 informants consist of traditional leaders, farmers, and Agriculture Extension Officers, and analyzed by multinomial logistic regression model. The results showed that the traditional rituals that required the sacrifice of pigs included the ceremonies marking the start of building residential and traditional community buildings and dowry (belis) presentation in a wedding ceremony. The decision-making in choosing the rearing practice was influenced by pig ownership, care system (P<0.01), and income (P<0.05). Farmers who have small scale will choose extensive and fattening systems to reduce feed costs. Those practicing breeding are still maintaining local breeds because traditional rituals require it. Further efforts are needed to increase the populations of local breeds as well as cross-bred pigs to maintain the ceremonies in traditional rituals and income generation.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"254 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140457833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Tirtasari, L. T. Suwanti, M. Mufasirin, P. Hastutiek, Elly Nur Indasari, R. Kurnijasanti, H. Plumeriastuti, Erma Safitri, E. Hestianah
Abstract. Tirtasari K, Suwanti LT, Mufasirin, Hastutiek P, Indasari EN, Kurnijasanti R, Plumeriastuti H, Safitri E, Hestianah EP. 2024. Phytochemical analysis and antioxidant activities ethanol extract of propolis Trigona spp. from different vegetation in Lombok, West Nusa Tenggara, Indonesia. Biodiversitas 25: 404-411. Propolis is a natural substance containing a resin that is collected by honey bees from various plants and has a significant effect on human health such as antibacterial, antifungal, anti-inflammatory, antiviral, anesthetic, antioxidant, antitumoural, antiprotozoal, anticancer, antihypertensive, anticarcinogenic, antihepatotoxic and has cytotoxic activity. Cultivation of Trigona spp. in Lombok is growing rapidly. Trigona spp. produces more propolis than honey bees of Apis spp., but bee products that are maximally utilized are only honey, and propolis has not been utilized optimally. Currently, there is no scientific validation of the phytochemical content, bioactive compounds, and biological activities of ethanol extract propolis Trigona spp. from Lombok, West Nusa Tenggara. So, this study aimed to determine the phytochemical compounds, bioactive compounds, and antioxidant activity of propolis. Gas Chromatography-Mass Spectrometry (GC-MS) analysis was performed to determine the bioactive compounds of ethanol extract propolis. DPPH (2,2-diphenyl-1-picrylhydrazyl) was used to evaluate antioxidant activity. The composition of propolis compounds is different for each propolis depending on the bee species and the vegetation source from which the resin was collected. In this research, propolis was collected from different vegetation sources (cashews, moringa, coffee, and lote trees). This study used qualitative and quantitative phytochemical analysis and the antioxidant DPPH test. Different components and percentages cause significant variations in the results obtained from phytochemical tests. The content is different in various propolis, there are saponins, flavonoids, and tannins. The highest antioxidant activity was found in propolis with moringa resin 38.4150 (very strong), followed by lote tree resin -66.0420 (strong), cashew resin 81.8140 (strong), and coffee resin 100.7465 (moderate).
{"title":"Phytochemical analysis and antioxidant activities ethanol extract of propolis Trigona spp. from different vegetation in Lombok, West Nusa Tenggara, Indonesia","authors":"K. Tirtasari, L. T. Suwanti, M. Mufasirin, P. Hastutiek, Elly Nur Indasari, R. Kurnijasanti, H. Plumeriastuti, Erma Safitri, E. Hestianah","doi":"10.13057/biodiv/d250147","DOIUrl":"https://doi.org/10.13057/biodiv/d250147","url":null,"abstract":"Abstract. Tirtasari K, Suwanti LT, Mufasirin, Hastutiek P, Indasari EN, Kurnijasanti R, Plumeriastuti H, Safitri E, Hestianah EP. 2024. Phytochemical analysis and antioxidant activities ethanol extract of propolis Trigona spp. from different vegetation in Lombok, West Nusa Tenggara, Indonesia. Biodiversitas 25: 404-411. Propolis is a natural substance containing a resin that is collected by honey bees from various plants and has a significant effect on human health such as antibacterial, antifungal, anti-inflammatory, antiviral, anesthetic, antioxidant, antitumoural, antiprotozoal, anticancer, antihypertensive, anticarcinogenic, antihepatotoxic and has cytotoxic activity. Cultivation of Trigona spp. in Lombok is growing rapidly. Trigona spp. produces more propolis than honey bees of Apis spp., but bee products that are maximally utilized are only honey, and propolis has not been utilized optimally. Currently, there is no scientific validation of the phytochemical content, bioactive compounds, and biological activities of ethanol extract propolis Trigona spp. from Lombok, West Nusa Tenggara. So, this study aimed to determine the phytochemical compounds, bioactive compounds, and antioxidant activity of propolis. Gas Chromatography-Mass Spectrometry (GC-MS) analysis was performed to determine the bioactive compounds of ethanol extract propolis. DPPH (2,2-diphenyl-1-picrylhydrazyl) was used to evaluate antioxidant activity. The composition of propolis compounds is different for each propolis depending on the bee species and the vegetation source from which the resin was collected. In this research, propolis was collected from different vegetation sources (cashews, moringa, coffee, and lote trees). This study used qualitative and quantitative phytochemical analysis and the antioxidant DPPH test. Different components and percentages cause significant variations in the results obtained from phytochemical tests. The content is different in various propolis, there are saponins, flavonoids, and tannins. The highest antioxidant activity was found in propolis with moringa resin 38.4150 (very strong), followed by lote tree resin -66.0420 (strong), cashew resin 81.8140 (strong), and coffee resin 100.7465 (moderate).","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140458943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Faizah Nur Shabrina, Kunto Wibowo, Tuty Arisuryanti
Abstract. Shabrina FN, Wibowo K, Arisuryanti T. 2024. Cryptic diversity of mudskipper genus Boleophthalmus (Gobiiformes: Oxudercidae) from the north coast of East Java, Indonesia. Biodiversitas 25: 412-420. Mudskippers of genus Boleophthalmus are commonly found in the Indo-Pacific area. This study aimed to examine the diversity of the genus Boleophthalmus from the north coast of East Java Province, Indonesia by employing the mitochondrial COI gene for DNA barcoding and morphological characterization. Ten individuals of this genus were analyzed. According to the similarity percentage evaluated using Basic Local Alignment Search Tool (BLAST) and Barcode of Life Data (BOLD) identification engines, all the individuals were identified as Boleophthalmus boddarti Pallas, 1770. However, phylogenetic analysis revealed that three of the individuals were identified as B. boddarti, and seven were identified as Boleophthalmus pectinirostris Linnaeus, 1758 originated from East Java, which is separated from the clade of B. pectinirostris from East Asia population. These results were supported by the genetic distance of 8.93%-13.81% and the morphological characteristics determined by principal component analysis (PCA). PCA revealed that B. boddarti and B. pectinirostris are primarily distinguished by the number of (longitudinal) scales (69-75 versus 113-123), length of second dorsal fin base (42.3-43.9 versus 38.1-49.0), and caudal fin length (18.1-22.4 versus 17.5-21.6). Further detailed studies are needed to clarify whether the seven individuals belonging to B. pectinirostris complex should be regarded as a new species or a new subspecies.
Abstract.Shabrina FN, Wibowo K, Arisuryanti T. 2024.印度尼西亚东爪哇岛北海岸弹涂鱼属Boleophthalmus（戈壁形目：Oxudercidae）的隐秘多样性。Biodiversitas 25: 412-420.弹涂鱼属 Boleophthalmus 常见于印度洋-太平洋地区。本研究旨在利用线粒体 COI 基因进行 DNA 条形编码和形态特征描述，研究印度尼西亚东爪哇省北海岸弹涂鱼属的多样性。对该属的 10 个个体进行了分析。根据基本局部比对搜索工具（BLAST）和生命条形码数据（BOLD）识别引擎评估的相似度，所有个体均被鉴定为 Boleophthalmus boddarti Pallas，1770。然而，系统进化分析表明，其中 3 个个体被鉴定为 B. boddarti，7 个个体被鉴定为 Boleophthalmus pectinirostris Linnaeus, 1758，它们来自东爪哇，与来自东亚种群的 B. pectinirostris 支系相分离。遗传距离（8.93%-13.81%）和主成分分析（PCA）确定的形态特征均支持上述结果。PCA 显示，B. boddarti 和 B. pectinirostris 主要通过（纵向）鳞片数量（69-75 对 113-123）、第二背鳍基部长度（42.3-43.9 对 38.1-49.0）和尾鳍长度（18.1-22.4 对 17.5-21.6）来区分。这 7 个属于 B. pectinirostris 复合物的个体应被视为新种还是新亚种，还需要进一步的详细研究。
{"title":"Cryptic diversity of mudskipper genus Boleophthalmus (Gobiiformes: Oxudercidae) from the north coast of East Java, Indonesia","authors":"Faizah Nur Shabrina, Kunto Wibowo, Tuty Arisuryanti","doi":"10.13057/biodiv/d250148","DOIUrl":"https://doi.org/10.13057/biodiv/d250148","url":null,"abstract":"Abstract. Shabrina FN, Wibowo K, Arisuryanti T. 2024. Cryptic diversity of mudskipper genus Boleophthalmus (Gobiiformes: Oxudercidae) from the north coast of East Java, Indonesia. Biodiversitas 25: 412-420. Mudskippers of genus Boleophthalmus are commonly found in the Indo-Pacific area. This study aimed to examine the diversity of the genus Boleophthalmus from the north coast of East Java Province, Indonesia by employing the mitochondrial COI gene for DNA barcoding and morphological characterization. Ten individuals of this genus were analyzed. According to the similarity percentage evaluated using Basic Local Alignment Search Tool (BLAST) and Barcode of Life Data (BOLD) identification engines, all the individuals were identified as Boleophthalmus boddarti Pallas, 1770. However, phylogenetic analysis revealed that three of the individuals were identified as B. boddarti, and seven were identified as Boleophthalmus pectinirostris Linnaeus, 1758 originated from East Java, which is separated from the clade of B. pectinirostris from East Asia population. These results were supported by the genetic distance of 8.93%-13.81% and the morphological characteristics determined by principal component analysis (PCA). PCA revealed that B. boddarti and B. pectinirostris are primarily distinguished by the number of (longitudinal) scales (69-75 versus 113-123), length of second dorsal fin base (42.3-43.9 versus 38.1-49.0), and caudal fin length (18.1-22.4 versus 17.5-21.6). Further detailed studies are needed to clarify whether the seven individuals belonging to B. pectinirostris complex should be regarded as a new species or a new subspecies.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"11 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140458889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ni’matuzahroh Ni’matuzahroh, M. Affandi, Agus Supriyanto, Berlian Rustantina, Laily Ainun Jaiyah, Aisyah Rahmawati, Hesti Nurhayati, S. K. Sari, A. M. Khiftiyah, Daman Huri
Abstract. Ni’matuzahroh, Affandi M, Supriyanto A, Rustantina B, Jaiyah LA, Rahmawati A, Nurhayati H, Sari SK, Khiftiyah AM, Huri D. 2024. Biodiversity of hydrolytic enzymes-producing soil bacteria from a Durian Park, Jombang, Indonesia: Beneficial prospect for sustainable agriculture. Biodiversitas 25: 392-403. Biofertilizer is an alternative to chemical fertilizer that can enhance plant growth but is specific to certain plants and soils. Various biofertilizers have been produced, but the specific biofertilizers to enhance durian growth have not been widely available. This study aims to determine the potential of hydrolytic enzyme-producing bacteria, one of the microbial components of biofertilizers, in the De Durian Park (DDP) area, Wonosalam, Jombang District, Indonesia. The bacteria were isolated using the selective medium plating technique. The obtained isolates were screened for their ability to produce cellulase, amylase, protease, and lipase. The clear zone around the colony indicated the ability of the isolates to produce hydrolytic enzymes, and then the clear zone index was obtained. The best three isolates for each enzyme production were characterized macroscopically, microscopically, and biochemically, then identified using 16S rRNA gene sequence analysis. Fifty-two isolates were successfully obtained from the soil of DDP. The best three cellulolytic bacteria had indices ranging from 1.83 to 2.14, categorized as moderate-strong, identified as Bacillus anthracis, B. pacificus, and B. cereus. The amylolytic bacteria ranged from 1.25 to 1.35, categorized as moderate, and identified as Bacillus anthracis, B. paranthracis, and B. paramycoides. The proteolytic bacteria ranged from 1.33 to 1.50, categorized as moderate, identified as B. pumilus, B. paramycoides, and B. cereus. The lipolytic bacteria ranged from 1.94 to 3.87, categorized as moderate-strong, and were identified as Enterobacter sichuanensis, B. pacificus, and E. asburiae. These cellulolytic, amylolytic, proteolytic, and lipolytic bacteria have the potency as candidates for a biofertilizer formula.
摘要Ni'matuzahroh, Affandi M, Supriyanto A, Rustantina B, Jaiyah LA, Rahmawati A, Nurhayati H, Sari SK, Khiftiyah AM, Huri D. 2024.印度尼西亚裕廊榴莲园中产生水解酶的土壤细菌的生物多样性：可持续农业的有益前景。Biodiversitas 25: 392-403.生物肥料是化肥的替代品，可促进植物生长，但对某些植物和土壤有特定的作用。目前已生产出多种生物肥料，但可促进榴莲生长的特定生物肥料尚未广泛使用。本研究旨在确定生物肥料的微生物成分之一--水解酶产生细菌在印度尼西亚裕廊县沃诺萨拉姆的榴莲公园（DDP）地区的潜力。采用选择性培养基平板技术分离了这些细菌。对获得的分离菌进行了筛选，以检测其产生纤维素酶、淀粉酶、蛋白酶和脂肪酶的能力。菌落周围的透明区表示分离菌产生水解酶的能力，然后得出透明区指数。对每种酶的最佳三个分离物进行宏观、微观和生化鉴定，然后使用 16S rRNA 基因序列分析进行鉴定。从 DDP 的土壤中成功获得了 52 个分离菌株。最好的三种纤维素分解菌的指数在 1.83 到 2.14 之间，被归类为中强，分别是炭疽芽孢杆菌、太平洋芽孢杆菌和蜡样芽孢杆菌。淀粉溶解菌的指数从 1.25 到 1.35 不等，属于中等，确定为炭疽芽孢杆菌、副炭疽芽孢杆菌和副酵母菌。蛋白质分解菌的范围在 1.33 至 1.50 之间，属于中等，确定为普米氏杆菌、副酵母菌和蜡样芽孢杆菌。脂肪分解菌从 1.94 到 3.87 不等，被归类为中-强，确定为四川肠杆菌、太平洋肠杆菌和阿斯布里亚肠杆菌。这些纤维素分解菌、淀粉分解菌、蛋白质分解菌和脂肪分解菌具有作为生物肥料配方候选菌的潜力。
{"title":"Biodiversity of hydrolytic enzymes-producing soil bacteria from a Durian Park, Jombang, Indonesia: Beneficial prospect for sustainable agriculture","authors":"Ni’matuzahroh Ni’matuzahroh, M. Affandi, Agus Supriyanto, Berlian Rustantina, Laily Ainun Jaiyah, Aisyah Rahmawati, Hesti Nurhayati, S. K. Sari, A. M. Khiftiyah, Daman Huri","doi":"10.13057/biodiv/d250146","DOIUrl":"https://doi.org/10.13057/biodiv/d250146","url":null,"abstract":"Abstract. Ni’matuzahroh, Affandi M, Supriyanto A, Rustantina B, Jaiyah LA, Rahmawati A, Nurhayati H, Sari SK, Khiftiyah AM, Huri D. 2024. Biodiversity of hydrolytic enzymes-producing soil bacteria from a Durian Park, Jombang, Indonesia: Beneficial prospect for sustainable agriculture. Biodiversitas 25: 392-403. Biofertilizer is an alternative to chemical fertilizer that can enhance plant growth but is specific to certain plants and soils. Various biofertilizers have been produced, but the specific biofertilizers to enhance durian growth have not been widely available. This study aims to determine the potential of hydrolytic enzyme-producing bacteria, one of the microbial components of biofertilizers, in the De Durian Park (DDP) area, Wonosalam, Jombang District, Indonesia. The bacteria were isolated using the selective medium plating technique. The obtained isolates were screened for their ability to produce cellulase, amylase, protease, and lipase. The clear zone around the colony indicated the ability of the isolates to produce hydrolytic enzymes, and then the clear zone index was obtained. The best three isolates for each enzyme production were characterized macroscopically, microscopically, and biochemically, then identified using 16S rRNA gene sequence analysis. Fifty-two isolates were successfully obtained from the soil of DDP. The best three cellulolytic bacteria had indices ranging from 1.83 to 2.14, categorized as moderate-strong, identified as Bacillus anthracis, B. pacificus, and B. cereus. The amylolytic bacteria ranged from 1.25 to 1.35, categorized as moderate, and identified as Bacillus anthracis, B. paranthracis, and B. paramycoides. The proteolytic bacteria ranged from 1.33 to 1.50, categorized as moderate, identified as B. pumilus, B. paramycoides, and B. cereus. The lipolytic bacteria ranged from 1.94 to 3.87, categorized as moderate-strong, and were identified as Enterobacter sichuanensis, B. pacificus, and E. asburiae. These cellulolytic, amylolytic, proteolytic, and lipolytic bacteria have the potency as candidates for a biofertilizer formula.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"18 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140459024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract. Iman MH, Kuswandi PC, Subrata SA. 2024. Genetic variation of the native Rusa deer (Rusa timorensis) in Java and Bali (Indonesia) as revealed using non-invasive sampling. Biodiversitas 25: 355-360. Rusa deer is a vulnerable species with a large geographic range but natively inhabits Java and Bali. Despite the wide distribution, its native population is declining, raising a concern about a small population's adverse genetic effect. It encourages genetic studies to provide baseline data that has been vacant recently. This research aimed to demonstrate an application of non-invasive sampling to collect DNA samples and a simple procedure to obtain and analyze genetic data for the Rusa deer. This research also aimed to provide genetic variation of the native deer population as baseline data. The research sites were Baluran, Alas Purwo in East Java, and Bali Barat national parks from which fecal samples were collected. Moreover, 20 DNA samples were isolated from the feces using a kit (Dneasy PowerSoil Pro from Qiagen) and amplified at the control region gene using a forward: AAACCAGAAAAGGAGAGCAAC and a reverse: TCATCTAGGCATTTTCAGTGCC primer. The amplicons were sequenced, and the number of Haplotypes (Hn), Haplotype diversity (Hd), nucleotide diversity (p), site polymorphism, and phylogeographic tree were determined. The result showed that all the sequences had coverage of 100% and identity >98% with the Rusa timorensis sequence available in the GenBank. Furthermore, we found Hn = 11, Hd = 0.88, p = 0.005 and 30 site polymorphisms. Therefore, compared to an introduced population, the Rusa deer has a richer Hd and higher site polymorphism but a poorer p. Furthermore, we found that the Baluran population had high Hd, p, and is possibly forming a distinct clade.
摘要： Iman MH, Kuswandi PC, Subrata SA.Iman MH, Kuswandi PC, Subrata SA.2024.印度尼西亚爪哇岛和巴厘岛原生鹿（Rusa timorensis）的遗传变异，通过非入侵性采样揭示。Biodiversitas 25: 355-360.鲁沙鹿是一种易危物种，地理分布广泛，但原生栖息于爪哇和巴厘岛。尽管分布广泛，但其原生种群数量却在减少，令人担忧小种群会对遗传产生不利影响。它鼓励进行基因研究，以提供最近空缺的基线数据。本研究旨在展示非侵入性采样法在收集 DNA 样本方面的应用，以及获取和分析鲁沙鹿遗传数据的简单程序。这项研究还旨在提供本地鹿群的遗传变异作为基线数据。研究地点是东爪哇的巴卢兰（Baluran）、阿拉斯普沃（Alas Purwo）和巴厘巴拉特（Bali Barat）国家公园，并从中采集了粪便样本。此外，使用试剂盒（Qiagen 公司的 Dneasy PowerSoil Pro）从粪便中分离出 20 份 DNA 样本，并使用正向扩增法扩增控制区基因：AAACCAGAAAAGGAGAGCAAC 和反向：TCATCTAGGCATTTTCAGTGCC引物扩增控制区基因。对扩增子进行测序，并测定了单倍型数目（Hn）、单倍型多样性（Hd）、核苷酸多样性（p）、位点多态性和系统地理树。结果表明，所有序列的覆盖率均为 100%，与 GenBank 中的 Rusa timorensis 序列的同一性大于 98%。此外，我们还发现了 Hn = 11、Hd = 0.88、p = 0.005 和 30 个位点的多态性。因此，与引进种群相比，鲁沙鹿具有更丰富的 Hd 和更高的位点多态性，但 p 较差。此外，我们还发现 Baluran 种群具有较高的 Hd 和 p，并可能形成一个独特的支系。
{"title":"Genetic variation of the native Rusa deer (Rusa timorensis) in Java and Bali (Indonesia) as revealed using non-invasive sampling","authors":"M. H. Iman, P. C. Kuswandi, S. Subrata","doi":"10.13057/biodiv/d250141","DOIUrl":"https://doi.org/10.13057/biodiv/d250141","url":null,"abstract":"Abstract. Iman MH, Kuswandi PC, Subrata SA. 2024. Genetic variation of the native Rusa deer (Rusa timorensis) in Java and Bali (Indonesia) as revealed using non-invasive sampling. Biodiversitas 25: 355-360. Rusa deer is a vulnerable species with a large geographic range but natively inhabits Java and Bali. Despite the wide distribution, its native population is declining, raising a concern about a small population's adverse genetic effect. It encourages genetic studies to provide baseline data that has been vacant recently. This research aimed to demonstrate an application of non-invasive sampling to collect DNA samples and a simple procedure to obtain and analyze genetic data for the Rusa deer. This research also aimed to provide genetic variation of the native deer population as baseline data. The research sites were Baluran, Alas Purwo in East Java, and Bali Barat national parks from which fecal samples were collected. Moreover, 20 DNA samples were isolated from the feces using a kit (Dneasy PowerSoil Pro from Qiagen) and amplified at the control region gene using a forward: AAACCAGAAAAGGAGAGCAAC and a reverse: TCATCTAGGCATTTTCAGTGCC primer. The amplicons were sequenced, and the number of Haplotypes (Hn), Haplotype diversity (Hd), nucleotide diversity (p), site polymorphism, and phylogeographic tree were determined. The result showed that all the sequences had coverage of 100% and identity >98% with the Rusa timorensis sequence available in the GenBank. Furthermore, we found Hn = 11, Hd = 0.88, p = 0.005 and 30 site polymorphisms. Therefore, compared to an introduced population, the Rusa deer has a richer Hd and higher site polymorphism but a poorer p. Furthermore, we found that the Baluran population had high Hd, p, and is possibly forming a distinct clade.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"84 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140459072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. V. Simamora, M. V. Hahuly, P. Nenotek, Y. R. Kana, M. Kasim, Roddialek Pollo, Antonius R. B. Ola, Fadlan Pramatana
Abstract. Simamora AV, Hahuly MV, Nenotek PS, Kana YR, Kasim M, Pollo R, Ola AR, Pramatana F. 2024. In vitro screening of fungal endophytes from sandalwood (Santalum album) as antagonists to phytopathogens. Biodiversitas 25: 361-371. Endophytic fungi possess bioactive compounds and generate secondary metabolites like their host plants. The phytochemical compounds found in sandalwood plants (Santalum album L.) are recognized for their anti-pathogenic properties, suggesting the potential of sandalwood endophytic fungi as effective anti-phytopathogens. Alternaria solani and Fusarium oxysporum threaten tomato plants, whereas Phytophthora palmivora is particularly detrimental to cocoa plants. The aims of this study were: (i) to obtain isolates of endophytic fungi from roots, stems, and leaves of sandalwood plants and (ii) to assess the ability of endophytic fungi as anti-phytopathogens in vitro. Endophytic fungi were isolated through a direct plating method and next by purification and identification. All obtained endophytic fungi underwent testing for their antagonistic potential against A. solani, F. oxysporum, and P. palmivora in vitro through the dual culture method. The inhibition percentage was analyzed using variance analysis and further examined with the 5% HSD test. The study successfully isolated 104 endophytic fungal isolates from the sandalwood; 29 isolates were from the roots, 33 from the stems, and 42 from the leaves. All endophytic isolates demonstrated the ability to inhibit the growth of A. solani, F. oxysporum, and P. palmivora in vitro, with various inhibition percentages ranging from 26.7 to 83.3%. Further research will concentrate on secondary metabolites from selected isolates showing inhibition levels exceeding 70%.
摘要Simamora AV, Hahuly MV, Nenotek PS, Kana YR, Kasim M, Pollo R, Ola AR, Pramatana F. 2024.作为植物病原体拮抗剂的檀香内生真菌的体外筛选。Biodiversitas 25: 361-371。内生真菌拥有生物活性化合物，并能像宿主植物一样产生次级代谢产物。在檀香植物（Santalum album L.）中发现的植物化学物质具有公认的抗病原性，这表明檀香内生真菌具有有效抗植物病原体的潜力。Alternaria solani 和 Fusarium oxysporum 威胁着番茄植物，而棕榈疫霉对可可植物尤其有害。本研究的目的是(i) 从檀香植物的根、茎和叶中分离内生真菌；(ii) 评估内生真菌在体外抗植物病原体的能力。内生真菌通过直接培养法进行分离，然后进行纯化和鉴定。所有获得的内生真菌都通过双重培养法进行了体外拮抗 A. solani、F. oxysporum 和 P. palmivora 的潜力测试。采用方差分析对抑制率进行了分析，并用 5% HSD 检验对抑制率进行了进一步检验。研究从檀香中成功分离出 104 个内生真菌分离物，其中 29 个来自根部，33 个来自茎部，42 个来自叶片。所有内生真菌分离物均能在体外抑制 A. solani、F. oxysporum 和 P. palmivora 的生长，抑制率从 26.7% 到 83.3% 不等。进一步的研究将集中于抑制率超过 70% 的部分分离物的次级代谢物。
{"title":"In vitro screening of fungal endophytes from sandalwood (Santalum album) as antagonists to phytopathogens","authors":"A. V. Simamora, M. V. Hahuly, P. Nenotek, Y. R. Kana, M. Kasim, Roddialek Pollo, Antonius R. B. Ola, Fadlan Pramatana","doi":"10.13057/biodiv/d250142","DOIUrl":"https://doi.org/10.13057/biodiv/d250142","url":null,"abstract":"Abstract. Simamora AV, Hahuly MV, Nenotek PS, Kana YR, Kasim M, Pollo R, Ola AR, Pramatana F. 2024. In vitro screening of fungal endophytes from sandalwood (Santalum album) as antagonists to phytopathogens. Biodiversitas 25: 361-371. Endophytic fungi possess bioactive compounds and generate secondary metabolites like their host plants. The phytochemical compounds found in sandalwood plants (Santalum album L.) are recognized for their anti-pathogenic properties, suggesting the potential of sandalwood endophytic fungi as effective anti-phytopathogens. Alternaria solani and Fusarium oxysporum threaten tomato plants, whereas Phytophthora palmivora is particularly detrimental to cocoa plants. The aims of this study were: (i) to obtain isolates of endophytic fungi from roots, stems, and leaves of sandalwood plants and (ii) to assess the ability of endophytic fungi as anti-phytopathogens in vitro. Endophytic fungi were isolated through a direct plating method and next by purification and identification. All obtained endophytic fungi underwent testing for their antagonistic potential against A. solani, F. oxysporum, and P. palmivora in vitro through the dual culture method. The inhibition percentage was analyzed using variance analysis and further examined with the 5% HSD test. The study successfully isolated 104 endophytic fungal isolates from the sandalwood; 29 isolates were from the roots, 33 from the stems, and 42 from the leaves. All endophytic isolates demonstrated the ability to inhibit the growth of A. solani, F. oxysporum, and P. palmivora in vitro, with various inhibition percentages ranging from 26.7 to 83.3%. Further research will concentrate on secondary metabolites from selected isolates showing inhibition levels exceeding 70%.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"149 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140459099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Solihin, D. A. Santosa, Budi Nugroho, P. Purwono, Rija Sudirja, H. Maulana, N. Kamaluddin, A. Karuniawan, Syaiful Anwar
Abstract. Solihin E, Santosa DA, Nugroho B, Purwono, Sudirja R, Maulana H, Kamaluddin NN, Karuniawan A, Anwar S. 2024. Selection for representative environment to identify high yield and sweetness levels of sweet potato (Ipomoea batatas) in Indonesia. Biodiversitas 25: 386-391. Sweet potato is one of the important and commercial agricultural commodities in the world. Yield and sweetness levels are important traits that can influence consumer preferences. Environment is an important factor in the development of sweet potatoes. A suitable environment will provide high yields and good quality. The objectives of this study were to examine the significance and relevance of sweet potato yield and sweetness levels in two locations (Sumedang and Bandung District) and to select representative environments for sweet potato yield and sweetness levels. The research was conducted in Sumedang and Bandung districts on lowland paddy field and dry land. The field experiment used a factorial randomized completed block design with three repetitions. The significance and relevance of sweet potato yield and sweetness levels was analysis using T-test. Representative environments were identified by GGE biplot. The results showed that the Bandung and Sumedang environments showed significant differences in yield but not in sweetness. The Bandung paddy field is the most representative environment for yield, while the Sumedang dry field and Bandung dry field are the most representative environments for sweetness. The results of this research can be used by researchers, farmers and industrial users in the sweet potato development program, especially the yield and yield quality.
摘要Solihin E, Santosa DA, Nugroho B, Purwono, Sudirja R, Maulana H, Kamaluddin NN, Karuniawan A, Anwar S. 2024.印度尼西亚甘薯（Ipomoea batatas）高产和甜度水平的代表性环境选择。Biodiversitas 25: 386-391.甘薯是世界上重要的商业农产品之一。产量和甜度是影响消费者喜好的重要特征。环境是甘薯发展的一个重要因素。适宜的环境将带来高产和优质。本研究的目的是考察两个地区（苏美当和万隆区）甘薯产量和甜度水平的重要性和相关性，并选择甘薯产量和甜度水平的代表性环境。研究在苏美当和万隆地区的低洼水田和旱地上进行。田间试验采用了三次重复的因子随机整群设计。甘薯产量和甜度水平的显著性和相关性采用 T 检验法进行分析。通过 GGE 双图确定了代表性环境。结果表明，Bandung 和 Sumedang 环境在产量上有显著差异，但在甜度上没有。万隆水田是产量最具代表性的环境，而苏美当旱田和万隆旱田则是甜度最具代表性的环境。这项研究的结果可供研究人员、农民和工业用户在甘薯开发项目中使用，尤其是在产量和产量质量方面。
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