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Anatomical Study of Male Reproductive Organs of the Indonesian Short-Nosed Fruit Bat (Cynopterus titthaecheilus Temminck, 1825) 印度尼西亚短鼻果蝠(Cynopterus titthaecheilus Temminck, 1825)雄性生殖器官的解剖研究
Pub Date : 2017-04-27 DOI: 10.14421/BIOMEDICH.2017.61.13-17
Anisatuzzahro Anisatuzzahro, Muhammad Ja’far Luthfi
Bats are one of the mammals of Chiroptera order. Chiroptera Order has two sub-orders, Megachiroptera and Microchiroptera. One of the species of Megachiroptera is Cynopterus titthaecheilus ( C. titthaecheilus ). Local name for C. titthaecheilus is the Indonesian short-nosed fruit bat . Characteristics of C. titthaecheilus are dark brown body hair and no tragus in the ear. Population of C. titthaecheilus in Indonesia are relatively abundant,  but research about anatomy of male reproduction organs of this species is still rare. The purpose of this study was to determine the anatomical features of male C. tittahecheilus reproductive organs. Observations of macroscopic anatomy include observation of morphology, size, weight and volume of penis, testiscle, epididymis, vas defferens, prostate and seminal vesicles. Observation on microscopical anatomy of male reproduction organ is using histological slide preparations stained by Hematoxylin-Eosin .
蝙蝠是翼手目的哺乳动物之一。翼手目有两个亚目,大翅目和小翅目。大翅目的其中一种是隐翅目(C.titthaecheilus)。C.titthaecheilus的当地名称是印度尼西亚的短鼻果蝙蝠。山竹的特征是体毛呈深棕色,耳朵里没有耳屏。印尼的山雀种群数量相对丰富,但对该物种雄性生殖器官解剖的研究仍然很少。本研究的目的是确定山雀雄性生殖器官的解剖特征。宏观解剖学观察包括阴茎、睾丸、附睾、输精管、前列腺和精囊的形态、大小、重量和体积的观察。用苏木精-伊红染色的组织学玻片对雄性生殖器官的显微解剖进行观察。
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引用次数: 0
Analysis of Bull Sperm DNA Abnormalities Due to Cadmium Accumulation 镉积累引起公牛精子DNA异常的分析
Pub Date : 2017-04-27 DOI: 10.14421/BIOMEDICH.2017.61.5-8
Fuad Fitriawan
Sperm abnormalities can occur by various causes. Abnormality of sperm is usually characterized by abnormal sperm motility and viability. This was caused by the inability of mitochondria on ATP-ase in producing ATP and ecto-enzyme Cik role in keeping the movement so that movement of sperm motility declines. Research that leads to total abnormal sperm DNA analysis is still rare. The purpose of this research was to get the results of the molecular characteristic picture of the overall characteristics of DNA loci that have abnormalities in bull sperm and get a picture of differences in overall DNA loci of abnormal and normal sperm. This research was conducted in October-December 2016. With the results of Group I consists of D, E with a percent similarity of 92.308%, group II consists of C and Group I with a percentage similarity of 50.125%, group II consists of A and B with a percent similarity of 100%, group II and group III with a percentage similarity of 0%. Based on the above data it can be concluded that the treatment A and B is not suspected to cause DNA damage compared to treatment C, D and E.
精子异常可由多种原因引起。精子异常通常以精子活力和活力异常为特征。这是由于线粒体在ATP酶上不能产生ATP和胞外酶Cik在保持运动中的作用,从而导致精子运动能力下降。导致精子DNA分析总体异常的研究仍然很少。本研究的目的是获得公牛精子中存在异常的DNA基因座的总体特征的分子特征图的结果,并获得异常精子和正常精子的总体DNA基因座差异的图像。本研究于2016年10月至12月进行。结果:第一组由D、E组成,相似度为92.308%,第二组由C和第一组组成,相似度50.125%,第II组由a和B组成,相似度100%,第II和第III组,相似度0%。基于上述数据,可以得出结论,与处理C、D和E相比,处理A和B不被怀疑会引起DNA损伤。
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引用次数: 1
Starch-Glycerol Based Edible Film and Effect of Rosella (Hibiscus Sabdariffa Linn) Extract and Surimi Dumbo Catfish (Clarias gariepinus) Addition on Its Mechanical Properties 淀粉-甘油基可食性薄膜及玫瑰茄提取物和鲣鱼提取物对薄膜力学性能的影响
Pub Date : 2016-10-24 DOI: 10.14421/BIOMEDICH.2016.52.33-40
E. Sedyadi, Syafiana Khusna Aini, D. Anggraini, D. Ekawati
Effect of Rosella ( Hibiscus Sabdariffa Linn) Extract and Surimi Dumbo catfish ( Clarias gariepinus ) addition on Starch-Based Edible Film-Glycerol Mechanical Properties has been done. The purpose of this study is to create an active environment-friendly packaging material. Surimi additions are intended to improve the mechanical properties of bioplastics and additions of Rosella extract intended as a bio-indicator of acidity. The method used was Solvent Casting. An amount of surimi and rosella extract varied to obtain the best mechanical properties. The results shows that the addition of surimi and rosella flower extract significantly effect the elongation of Edible films produced up to 27%.
研究了玫瑰茄(Hibiscus Sabdariffa Linn)提取物和鲣鱼(Clarias gariepinus)对淀粉基食用膜甘油力学性能的影响。本研究的目的是创造一种有效的环保包装材料。鱼糜的添加是为了改善生物塑料的机械性能,而迷迭香提取物的添加是为了作为酸度的生物指示剂。采用溶剂铸造法。鱼糜和迷迭香提取物的添加量不同,可获得最佳的力学性能。结果表明,鱼糜和迷迭花提取物的加入对食用膜伸长率有显著影响,可达27%。
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引用次数: 7
Effect of Addition of Soursop Leaf Extract to Ganyong (Canna edulis Ker.) Starch Edible Film and its application in Red Grape Storage Time 番荔枝叶提取物对甘永(美人蕉)的影响淀粉可食膜及其在红葡萄贮藏中的应用
Pub Date : 2016-10-24 DOI: 10.14421/BIOMEDICH.2016.52.55-59
E. Widyastuti, E. Sedyadi, S. Y. Prabawati
This study aimed to determine the effect of addition of Soursop leaf extract to  edible film and its effect on the storage time of red grapes. This research had three main steps, soursop leaf extraction, edible film making, and application on red grapes storage time. Soursop leaves extract variation used in this research are 0.5; 1; and 1.5% (w/w total) and the best result was used to coat the red grapes. The results showed that the optimum composition was obtained on the edible film with the addition of 0.5% (w/w total) soursop leaf extract. The composition increased film thickness from 0.03 to 0.08 mm, decreased film’s tensile strength from 11.89 to 8.42 MPa, decreased elongation from 12.71 to 11.03%, decreased the Young Modulus from 0.935 to 0.764 MPa, and decreased the vapor transmission rate from 7.45 to 6.55 g/m 2 .hour. The 50% shrinkage of weight and 50% texture damage are used as parameters to measure the Red grapes storage time. Based on weight shrinkage, red grapes storage time change from 24 days to 29 days by using an edible film coating without addition of extract, an extend to 32 days while using an edible film coating with addition of soursop leaf extract. Based on texture damage, red grapes storage time increased from 13 to 41 days if using an edible film coating without addition of extract, and increased to 40 days using an edible film coating with the addition of soursop leaf extract.
本研究旨在研究番荔枝叶提取物对红葡萄食用膜的影响及其对红葡萄贮藏时间的影响。本研究主要分为三个步骤,分别是酸参叶提取、食用膜制作和红葡萄贮藏时间的应用。本研究中使用的刺果叶提取物变异量为0.5;1;以总用量1.5% (w/w)涂覆红葡萄效果最佳。结果表明,在食用膜上添加0.5% (w/w总量)的番荔枝叶提取物,获得了最佳的配方。该组分使薄膜厚度从0.03 mm增加到0.08 mm,拉伸强度从11.89 MPa降低到8.42 MPa,伸长率从12.71降低到11.03%,杨氏模量从0.935降低到0.764 MPa,水蒸气透过率从7.45降低到6.55 g/m 2 h。以50%的收缩率和50%的织构损伤作为衡量红葡萄贮藏时间的参数。根据收缩率,未添加提取物的可食膜包衣组的红葡萄贮藏时间由24天延长至29天,添加刺草叶提取物的可食膜包衣组的贮藏时间延长至32天。以质地损伤为基础,不添加提取物的可食膜涂膜可使红葡萄的贮藏时间从13天增加到41天,添加刺草叶提取物的可食膜涂膜可使红葡萄的贮藏时间增加到40天。
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引用次数: 2
Optimization of Binocular Microscope with Micro Digital Camera for Measuring Seminiferous Tubules Epithelium Height 微型数码相机双筒显微镜测量精小管上皮高度的优化
Pub Date : 2016-10-24 DOI: 10.14421/BIOMEDICH.2016.52.41-47
Sutriyono Sutriyono
Binocular microscope optimization using micro digital camera to measure seminiferous tubules epithelium of mouse testis ( Mus muculus ) was completed. This research was conducted at Biology Laboratory of Faculty of Science and Technology UIN Sunan Kalijaga. The purpose of this study was to determine the height of the seminiferous tubules epithelium of mouse testis using micro digital camera. A computer was connected with binocular microscope and optilab advance, then calibration of optilab advance were done on objective lens magnification at 4x, 10x and 40x with 10x ocular lens. The analysis used was descriptive analysis. The mean of  the seminiferous tubules epithelium height is 105.6 μm. Optimization on computer and binocular microscope with micro digital camera can be used to measure seminiferous tubules epithelium.
利用微型数码相机对小鼠睾丸精管上皮的测量进行了双目显微镜优化。这项研究是在苏南卡利加大学科学技术学院生物实验室进行的。本研究的目的是利用微型数码相机测定小鼠睾丸精小管上皮的高度。将计算机与双目显微镜和optilab advance连接,然后用10倍目镜对物镜在4倍、10倍和40倍倍率下进行optilab advance标定。采用描述性分析。精小管上皮平均高度为105.6 μm。利用计算机优化和带微型数码相机的双目显微镜可以测量精小管上皮。
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引用次数: 1
A Stability Mathematical Model of Nasopharyngeal Carcinoma on Cellular Level 鼻咽癌细胞水平的稳定数学模型
Pub Date : 2016-10-24 DOI: 10.14421/BIOMEDICH.2016.52.61-64
S. Sugiyanto, F. A. Kusumo, L. Aryati, M. Hardianti
This paper discussed the stability of “Tumorigenesis Models” to link between EBV and carcinoma of the nasopharyngeal from normal cell to invasive carcinoma. The review on this case accomplished the previous theorem of equilibrium point on “Tumorigenesis Models”.
本文讨论了EBV与鼻咽癌从正常细胞到侵袭性癌的“肿瘤发生模型”的稳定性。本病例的复习完成了以往“肿瘤发生模型”的平衡点定理。
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引用次数: 0
Histological Study of Common House Gecko (Hemidactylus frenatus) Regenerated Tail 普通壁虎再生尾的组织学研究
Pub Date : 2016-10-24 DOI: 10.14421/BIOMEDICH.2016.52.49-53
Rakhmiyati Rakhmiyati, Muhammad Ja’far Luthfi
Common house gecko ( Hemidactylus frenatus ) belongs to suborder Lacertilia that has capacity to shed its tail (autotomy) as a self-defense mechanism. After autotomy, tail regeneration occurs. Axiale skeleton of an original tail is composed of bony vertebrae, whereas the the regenerate one is comprised of cartilaginous tube. The purpose of this study was to determine the histological difference between axial skeleton of the original tail and the regenerate one of the common house gecko. Twenty four individuals consist of twelve common house gecko with original tail and twelve with regenerate tail were used. Microanatomical observations were carried on histological slide of original and regenerated tail stained with Hematoxylin-Eosin and Mallory Acid Fuchsin. The results showed that the original tail comprised of bony vertebrae whereas regenerated tail supported by cartilaginous tube.
普通壁虎(Hemidactylus frenatus)属于裂尾亚目,具有脱尾(自切)作为自卫机制的能力。自切后,尾巴再生。原始尾巴的轴骨由骨性椎骨组成,而再生尾巴由软骨管组成。本研究的目的是确定原始尾轴骨骼与再生尾轴骨骼的组织学差异。实验共24只,包括12只原尾普通壁虎和12只再生尾壁虎。用苏木精-伊红和茂萝酸品红染色对原尾和再生尾的组织学切片进行显微解剖观察。结果表明,原尾由骨性椎体组成,再生尾由软骨管支撑。
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引用次数: 3
Checklist of Macroalgae in Waisai Coast, Raja Ampat Raja Ampat的Waisai海岸大型藻类清单
Pub Date : 2016-05-01 DOI: 10.14421/BIOMEDICH.2016.51.23-32
R. Suryandari, W. Widodo
Macroalgae are very abundant organisms in Indonesian coastal zone. They comprise 8.6% of the total marine organisms.The aim of the research was to identify macroalgae in Waisai Coast Raja Ampat. The results showed that 38 macroalgae were found in Waisai Coast Raja Ampat but only 29 species of macroalgae can be identified. Macroalgae found in Waisai Coast Raja Ampat are green algae, red algae and brown algae. Green algae found and identified are Caulerpa macra (Weber-van Bosse) Draisma & Prud’homme, Caulerpa racemosa var. macrophysa (Sonder wx Kutzing) W.R.Taylor,  Caulerpa sertularoides (S. Gmelin) Howe f. brevipes (J. Agardh Svedilus), Caulerpa cupressoides (Vahl) C. Agardh, Halimeda discoidea Decaisne, Halimeda Opuntia (Linnaeus) J.V. Lamoroux, Halimeda tuna (J. Ellis & Solander) J.V. Lamoroux, Halimeda cylindraceae Decaisne, Halimeda macroloba Decaisne, Avrainvillea erecta (Berkeley) A. Gepp & E.S. Gepp, Codium geppiorum O.C.Schmidt, Boergesenia forbesii (Hardvey) Feldmann, Valonia ventricosa J. Agardh, Dictyosphaeria cavernosa (Forsskal) Borgesen, Chaetomorpha spiralis Okamura, Anadyomene wrightii Harvey ex. J. E. Gray, Neomeris annulata Dickie. Red algae species found and successfully identified are Acanthophora spicifera (M. Vahl) Borgesen, Laurencia papilosa (C. Agardh) Greville, Gracilaria salicornia (C. Agardh) E.Y. Dawson, Amphiora fragilissima (Linnaeus) J.V. Lamoroux, Hypnea pannosa J. Agardh. Brown algae species  found and identified are Hormophysa cuneiformis (J.F. Gmelin) P.C. Silva, Sargassum aquifolium (Turner) C. Agardh, Sargassum polycystum C. Agardh, Turbinaria ornata (Turner) J. Agardh, Padina australis Hauck, Canistrocarpus cervicornis (Kutzing) De Paula & De Clerck Hydroclathrus clatratus (C. Agardh ) M. Howe. The only species found in Indonesia is Sargassum aquifolium.
大型藻类是印度尼西亚沿海地区非常丰富的生物。它们占海洋生物总数的8.6%。该研究的目的是鉴定Raja Ampat的Waisai海岸的大型藻类。结果表明,拉加安帕省怀赛海岸共发现38种大型藻类,但可鉴定的大型藻类仅有29种。在怀赛海岸拉加安帕发现的大型藻类有绿藻、红藻和褐藻。已发现和鉴定的绿藻有:大锅藻(Weber-van Bosse) Draisma & Prud 'homme,总状锅藻var. macrophysa (Sonder wx Kutzing) W.R.Taylor, sertularoides (S. Gmelin) Howe f. brevipes (J. Agardh Svedilus),柏树锅藻(Vahl) C. Agardh, halmeda disidea Decaisne, halmeda Opuntia (Linnaeus) J.V. Lamoroux, halmeda tuna (J. Ellis & Solander) J.V. Lamoroux, halmeda aceae Decaisne, halmeda macroloba Decaisne,竖叶菊(Berkeley) A. Gepp & E. s . Gepp,叶菊(Codium geppiorum) O.C.Schmidt, forbesii (Hardvey) Feldmann, Valonia . Agardh, Dictyosphaeria cavernosa (Forsskal) Borgesen, Chaetomorpha spiralis Okamura, Anadyomene wrightii Harvey ex. J. E. Gray, Neomeris annulata Dickie。已发现并成功鉴定的红藻有棘藻(Acanthophora spicifera, M. Vahl) Borgesen、Laurencia papilosa (C. Agardh) Greville、水杨花(Gracilaria salicornia, C. Agardh) E.Y. Dawson、Amphiora fragilissima (Linnaeus) J.V. Lamoroux、Hypnea pannosa J. Agardh。发现和鉴定的褐藻种类有:角形藻(horophysa cuneiformis, J.F. Gmelin) P.C. Silva、水藻(Sargassum aquifolium, Turner) C. Agardh、多囊藻C. Agardh、Turbinaria ornata (Turner) J. Agardh、南藻Hauck、Canistrocarpus cervicornis (Kutzing) De Paula和De Clerck Hydroclathrus clatratus (C. Agardh) M. Howe。在印度尼西亚发现的唯一物种是马尾藻。
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引用次数: 0
New Record Marsdenia tenacissima (Asclepiadoideae, Apocynaceae) In Gunung Ijo Baturagung Yogyakarta Yogyakarta Gunung Ijo Baturagung的新记录
Pub Date : 2016-04-15 DOI: 10.14421/BIOMEDICH.2016.51.1-8
Widodo Widodo, Muhammad Ja’far Luthfi
Marsdenia tenacissima population were found among wild bushes at S 07 o 47’ 03.4”; E 110 o 30’ 48.0” about 415 meter above sea level in Gunung Ijo Baturagung Yogyakarta. Identification was based on literature and herbarium specimen. The research was conduct using exploration methods, morphoanatomical observation, and specimen collection. Marsdenia tenacissima in Jawa was not reported in Flora of Java. Marsdenia tenacissima habitus was liana. The specific character for its identification was pollinia’s structure. This paper presented other important character namely leaf, stem, flower, pollinia, and fruit of Marsdenia tenacissima in Gunung Ijo Baturagung Yogyakarta. Accurate description and examination of any plant species were needed for its conservation and awareness of public to local biodiversity.
在S 07 ~ 47′03.4′的野生灌木中发现了马氏藤种群;东经110°30 ' 48.0 ",海拔约415米。鉴定依据文献和植物标本室标本。采用勘探法、形态解剖观察、标本采集等方法进行研究。《爪哇植物志》中未见爪哇地区的马丝denia tenacissima。马氏藤属藤本植物。其鉴定的具体特征是花粉的结构。本文介绍了位于日惹的马氏藤(Marsdenia tenacissima)的其他重要性状,即叶、茎、花、授粉和果实。任何一种植物都需要对其进行准确的描述和检查,以保护其物种,提高公众对当地生物多样性的认识。
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引用次数: 1
Modified Alizarin Red S-Alcian Blue Staining for Reptilian Skeleton 爬虫类骨骼改良茜素红s -阿利新蓝染色
Pub Date : 2016-04-15 DOI: 10.14421/BIOMEDICH.2016.51.19-22
Muhammad Ja’far Luthfi
Skeletal staining is an important method in anatomical study. The aim of the research was to develop staining and clearing method of Reptilian skeleton using Alizarin Red S-Alcian Blue. The specimen were eviscerated, fixed, stained, cleared, and keep in glycerine solution. This modified double-staining has successfully stain bone and cartilage of Reptilian.
骨骼染色是解剖学研究的重要方法。本研究的目的是建立一种利用茜素红和阿利新蓝对爬行动物骨骼进行染色和清除的方法。标本被取出,固定,染色,清除,保存在甘油溶液中。这种改良的双染色方法已成功地对爬行动物的骨和软骨进行了染色。
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引用次数: 0
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Biology, Medicine, & Natural Product Chemistry
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