Pub Date : 2018-10-31DOI: 10.14421/BIOMEDICH.2018.72.45-49
Bayu Setya Aji Nugraha, W. Widodo, Rendi Yuntara, Normalita Normalita
Nglanggeran is a place that has a high plant diversity and there are many unique and unidentified wild plants. This study aims to list liliopside class plants which found around the climbing route. The angiosperm plants in the liliopside class found around the climbing route were successfully identified and consisted of 40 species belong to 17 families.
{"title":"Diversity of Angiospermae Plant Class Liliopsida in Mount Nglanggeran","authors":"Bayu Setya Aji Nugraha, W. Widodo, Rendi Yuntara, Normalita Normalita","doi":"10.14421/BIOMEDICH.2018.72.45-49","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2018.72.45-49","url":null,"abstract":"Nglanggeran is a place that has a high plant diversity and there are many unique and unidentified wild plants. This study aims to list liliopside class plants which found around the climbing route. The angiosperm plants in the liliopside class found around the climbing route were successfully identified and consisted of 40 species belong to 17 families.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81815542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-31DOI: 10.14421/BIOMEDICH.2018.72.33-38
Tiara Nur Elfiana, A. Fitria, E. Sedyadi, S. Y. Prabawati, Irwan Nugraha
Starch is known as a biodegradable raw material that can be degraded by bacteria and microorganisms in the soil. Starch has cellulose which is kind of plant cellulose. This study shows the biodegradation rates of plastic made from Ganyong Canna (Canna edulis Kerr) as a cellulose source which is added with nata de coco as a filler. The biodegradable plastic functional group was confirmed by using FITR. The results show that the O-H group of Ganyong Canna (Canna edulis Kerr) biodegradable plastic is located at wave number 3298.03 cm-1 and shifted to 3290.32 cm-1 after addition of nata de coco. The C-H bonds functional groups in Canna biodegradable plastics and nata de coco plastics are at wave numbers 2920.01 cm-1 and 2916.16 cm-1. While the C-O bonds functional groups in biodegradable starch plastics and nata de coco is shown at wave numbers 995.05 cm-1. The mechanical properties of biodegradable plastics testing are thickness, tensile strength, and elongation based on the ASTM method. The thickness is about 0.1005 mm, the tensile strength of biodegradable plastic is 4,3244 MPa and the elongation value range about 13.9639% while the WVTR range about 14.20 g/m² hours. The results show that the increase of the plastic degradation made from nata de coco occurs between 5% - 38% per days. It is faster than the plastic made from pure Ganyong Canna (Canna edulis Kerr) starch. These results indicate that nata de coco could be added in biodegradable plastic on packaging materials for better degradation.
{"title":"Degradation Study of Biodegradable Plastic Using Nata De Coco as A Filler","authors":"Tiara Nur Elfiana, A. Fitria, E. Sedyadi, S. Y. Prabawati, Irwan Nugraha","doi":"10.14421/BIOMEDICH.2018.72.33-38","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2018.72.33-38","url":null,"abstract":"Starch is known as a biodegradable raw material that can be degraded by bacteria and microorganisms in the soil. Starch has cellulose which is kind of plant cellulose. This study shows the biodegradation rates of plastic made from Ganyong Canna (Canna edulis Kerr) as a cellulose source which is added with nata de coco as a filler. The biodegradable plastic functional group was confirmed by using FITR. The results show that the O-H group of Ganyong Canna (Canna edulis Kerr) biodegradable plastic is located at wave number 3298.03 cm-1 and shifted to 3290.32 cm-1 after addition of nata de coco. The C-H bonds functional groups in Canna biodegradable plastics and nata de coco plastics are at wave numbers 2920.01 cm-1 and 2916.16 cm-1. While the C-O bonds functional groups in biodegradable starch plastics and nata de coco is shown at wave numbers 995.05 cm-1. The mechanical properties of biodegradable plastics testing are thickness, tensile strength, and elongation based on the ASTM method. The thickness is about 0.1005 mm, the tensile strength of biodegradable plastic is 4,3244 MPa and the elongation value range about 13.9639% while the WVTR range about 14.20 g/m² hours. The results show that the increase of the plastic degradation made from nata de coco occurs between 5% - 38% per days. It is faster than the plastic made from pure Ganyong Canna (Canna edulis Kerr) starch. These results indicate that nata de coco could be added in biodegradable plastic on packaging materials for better degradation.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73449837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-31DOI: 10.14421/BIOMEDICH.2018.72.57-59
Rakhmiyati Rakhmiyati, Muhammad Ja’far Luthfi
Common House Gecko (Hemidactylus frenatus) is one of reptiles that have ability to autotomy their tails. Tail autotomy is a mechanism to protect it self from predators. After the tail broke, there will be wound healing on the tail which is then followed by a tail regeneration event. Original tail and regenerate tail is very different morphologically and anatomically. The original tail is composed of bones while the tail of the regenerate is composed of cartilage. Histochemical staining using Alizarin Red-S Alcian Blue was done to differentiate bone and cartilage. This method will stained bones red while the cartilage will stained blue.
普通壁虎(半爪虎)是一种能够自切尾巴的爬行动物。尾巴自切是一种保护自己免受捕食者伤害的机制。尾巴断了之后，会有一个伤口愈合的尾巴，然后是一个尾巴再生事件。原尾和再生尾在形态学和解剖学上都有很大的不同。原来的尾巴由骨头组成，而再生的尾巴由软骨组成。用茜素红- s -阿利新蓝进行组织化学染色以区分骨和软骨。这种方法会把骨头染成红色，而把软骨染成蓝色。
{"title":"Alizarin Red S-Alcian Blue Staining for Regenerated tail of Common House Gecko (Hemidactylus frenatus)","authors":"Rakhmiyati Rakhmiyati, Muhammad Ja’far Luthfi","doi":"10.14421/BIOMEDICH.2018.72.57-59","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2018.72.57-59","url":null,"abstract":"Common House Gecko (Hemidactylus frenatus) is one of reptiles that have ability to autotomy their tails. Tail autotomy is a mechanism to protect it self from predators. After the tail broke, there will be wound healing on the tail which is then followed by a tail regeneration event. Original tail and regenerate tail is very different morphologically and anatomically. The original tail is composed of bones while the tail of the regenerate is composed of cartilage. Histochemical staining using Alizarin Red-S Alcian Blue was done to differentiate bone and cartilage. This method will stained bones red while the cartilage will stained blue.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81269330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-31DOI: 10.14421/BIOMEDICH.2018.72.51-55
S. Sugiyanto, L. Aryati, F. A. Kusumo, M. Hardianti
Nasopharyngeal Carcinoma (NPC) is a cancer that occurs in nasopharynx which is associated with Epstein-Barr Virus (EBV). Mutation agents in nasopharyngeal neoplasms occur because of EBV infection. Transformation of B-cells due to EBV causes hormone imbalance in lymphoid cells or nasopharyngeal epithelial tissue. Rates of EBV infection have been shown to be prognostic to NPC. The basic level of EBV DNA can be used for stratification prognosis, with higher titers showing greater disease severity and worse outcomes. With mathematical models, there is a correlation between the increase in Epstein-Barr Virus and the increase in Invasive Carcinoma Cells or increase in Nasopharyngeal Carcinoma Cells.
{"title":"Link of Nasopharyngeal Carcinoma and Epstein-Barr Virus","authors":"S. Sugiyanto, L. Aryati, F. A. Kusumo, M. Hardianti","doi":"10.14421/BIOMEDICH.2018.72.51-55","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2018.72.51-55","url":null,"abstract":"Nasopharyngeal Carcinoma (NPC) is a cancer that occurs in nasopharynx which is associated with Epstein-Barr Virus (EBV). Mutation agents in nasopharyngeal neoplasms occur because of EBV infection. Transformation of B-cells due to EBV causes hormone imbalance in lymphoid cells or nasopharyngeal epithelial tissue. Rates of EBV infection have been shown to be prognostic to NPC. The basic level of EBV DNA can be used for stratification prognosis, with higher titers showing greater disease severity and worse outcomes. With mathematical models, there is a correlation between the increase in Epstein-Barr Virus and the increase in Invasive Carcinoma Cells or increase in Nasopharyngeal Carcinoma Cells.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75889910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-31DOI: 10.14421/BIOMEDICH.2018.72.39-43
Ina Karlina, Muhammad Ja’far Luthfi
Dumbo catfish (Clarias gariepinus) and Gabus (Channa striata) have additional organ respiratory system structures in the gills, called labyrinth. This organ is a tool for fish to take oxygen directly from the air so that it can live in low oxygen habitats. Both fish have differences at the Order level. Catfish is an order of Siluriformes as Gabus is an order of Perciformes. The purpose of this study was to distinguish the anatomy and histology of the gill structure and labyrinth in both fish. The macroanatomy observation was conveyed by dissection to determine the gill topography in the two fish species. Histology preparations were carried out using the paraffin method and using Hematoxylin-Eosin (HE) staining that showed in the labyrinth organ there were many blood vessels to bind oxygen then distributed to all parts of the fish's body.
{"title":"Comparative Anatomy of Labyrinth and Gill of Catfish (Clarias gariepinus) (Burchell, 1822) and Snakehead Fish (Channa striata) (Bloch, 1793)","authors":"Ina Karlina, Muhammad Ja’far Luthfi","doi":"10.14421/BIOMEDICH.2018.72.39-43","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2018.72.39-43","url":null,"abstract":"Dumbo catfish (Clarias gariepinus) and Gabus (Channa striata) have additional organ respiratory system structures in the gills, called labyrinth. This organ is a tool for fish to take oxygen directly from the air so that it can live in low oxygen habitats. Both fish have differences at the Order level. Catfish is an order of Siluriformes as Gabus is an order of Perciformes. The purpose of this study was to distinguish the anatomy and histology of the gill structure and labyrinth in both fish. The macroanatomy observation was conveyed by dissection to determine the gill topography in the two fish species. Histology preparations were carried out using the paraffin method and using Hematoxylin-Eosin (HE) staining that showed in the labyrinth organ there were many blood vessels to bind oxygen then distributed to all parts of the fish's body.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78129932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-30DOI: 10.14421/BIOMEDICH.2018.71.5-13
E. Semiarti, E. Mursyanti, A. Suyoko, Faiza Senja Widya Perdana, Catharina Tri Widyastuti, A. N. Subchan
Orchid is an elegant ornamental plant and favoured by the society. Phalaenopsis "Sogo vivien" is a mini-sized orchid with an interesting white-striped purple petals. This study was aimed to analyze the stability of the integration of embryonic gene carrier T-DNA from Arabidobsis AtRKD4 into the P. "Sogo vivien" genome produced in 2016. The study was conducted in 3 stages: 1) Transgenic plant phenotype analysis (1 year old); 2) Examination of T-DNA integration in orchid genotypes using PCR. 3) Analysis of transgenic plant leaf explants’ ability to produce somatic embryo in vitro. In vitro cultures were performed on the base medium of New Phalaenopsis (NP), plus various concentrations of TDZ (0, 1, 2 mg.L -1 ) and IBA (0, 1, 2 mg.L -1 ) or without TDZ and IBA as controls. The transgenic Phalaenopsis ‘Sogo vivien’ were transferred to pot mediums via ex vitro with two treatments: the first leaves were cut as explants for in vitro culture, and the plants were transferred to the mixture of fern medium with shavings of bark. The integration of T-DNA in the genome was detected by DNA genome amplification from the second leaves using the AtRKD4 gene primers and the POH1 gene. The results showed that the highest number of somatic embryo (SE) propagules or protocorm like bodies (PLBs) amounted to 27 were derived from transgenic plant # 2 cultured on NP + 2 mg.L -1 TDZ +1 mg.L -1 IBA medium. The presence of AtRKD4 transgenes were detected with the amplification of 380 bp of the RKD4 gene from the genome of transgenic plant # 2 by using PCR. There were 2 out of 15 plants that positively carry the AtRKD4 gene and produce SE. Thus, the stability of the AtRKD4 carrier T-DNA integration in the genomes of transgenic plants was 13.3%.
{"title":"Stability of T-DNA Integration in Phalaenopsis “Sogo Vivien” Transgenic Orchid Carrying 35S::Gal4::AtRKD4::GR","authors":"E. Semiarti, E. Mursyanti, A. Suyoko, Faiza Senja Widya Perdana, Catharina Tri Widyastuti, A. N. Subchan","doi":"10.14421/BIOMEDICH.2018.71.5-13","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2018.71.5-13","url":null,"abstract":"Orchid is an elegant ornamental plant and favoured by the society. Phalaenopsis \"Sogo vivien\" is a mini-sized orchid with an interesting white-striped purple petals. This study was aimed to analyze the stability of the integration of embryonic gene carrier T-DNA from Arabidobsis AtRKD4 into the P. \"Sogo vivien\" genome produced in 2016. The study was conducted in 3 stages: 1) Transgenic plant phenotype analysis (1 year old); 2) Examination of T-DNA integration in orchid genotypes using PCR. 3) Analysis of transgenic plant leaf explants’ ability to produce somatic embryo in vitro. In vitro cultures were performed on the base medium of New Phalaenopsis (NP), plus various concentrations of TDZ (0, 1, 2 mg.L -1 ) and IBA (0, 1, 2 mg.L -1 ) or without TDZ and IBA as controls. The transgenic Phalaenopsis ‘Sogo vivien’ were transferred to pot mediums via ex vitro with two treatments: the first leaves were cut as explants for in vitro culture, and the plants were transferred to the mixture of fern medium with shavings of bark. The integration of T-DNA in the genome was detected by DNA genome amplification from the second leaves using the AtRKD4 gene primers and the POH1 gene. The results showed that the highest number of somatic embryo (SE) propagules or protocorm like bodies (PLBs) amounted to 27 were derived from transgenic plant # 2 cultured on NP + 2 mg.L -1 TDZ +1 mg.L -1 IBA medium. The presence of AtRKD4 transgenes were detected with the amplification of 380 bp of the RKD4 gene from the genome of transgenic plant # 2 by using PCR. There were 2 out of 15 plants that positively carry the AtRKD4 gene and produce SE. Thus, the stability of the AtRKD4 carrier T-DNA integration in the genomes of transgenic plants was 13.3%.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48811323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-30DOI: 10.14421/BIOMEDICH.2018.71.27-31
M. Amin, K. Putra, I. F. Amin, N. Earlia, Dina Maulina, B. Lukiati, U. Lestari
Inflammation is a tissue injury that occurs due to physical trauma or microbiological substances that involve the activities of many cell types. Inflammation can be prevented using the natural medicines from Allium cepa L. Quercetin is one of the bioactive compounds found in Allium cepa L and has been reported to have anti-inflammatory activity. The natural medicines have been used to minimize non-steroidal anti-inflammatory drugs. This study aims to investigated the modeling structures and the protein receptor from quecertin in inflammation mechanism and their optimization of the effectiveness in the human body. The bioinformatics tools used in this study are the database of quercetin compounds, Pubchem and Swis Target Prediction protein prediction databases, PyRx 0.8 molecular docking software, ligand docking, and binding site analysis with PyMOL and LigPlus software. The results from in silico show that quercetin compounds can interact with Muscleblind-like protein 1 target protein with a Binding Affinity minus value which is not much different from the dexamethasone compound. Dexamethason is a standart because it is a corticosteroid drug that can be used as an anti-inflammatory to reduce inflammation, allergic reactions, arthritis and other inflammatory diseases.
{"title":"Quercetin: the bioactive compound from Allium cepa L. as anti-inflammation based on in silico screening","authors":"M. Amin, K. Putra, I. F. Amin, N. Earlia, Dina Maulina, B. Lukiati, U. Lestari","doi":"10.14421/BIOMEDICH.2018.71.27-31","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2018.71.27-31","url":null,"abstract":"Inflammation is a tissue injury that occurs due to physical trauma or microbiological substances that involve the activities of many cell types. Inflammation can be prevented using the natural medicines from Allium cepa L. Quercetin is one of the bioactive compounds found in Allium cepa L and has been reported to have anti-inflammatory activity. The natural medicines have been used to minimize non-steroidal anti-inflammatory drugs. This study aims to investigated the modeling structures and the protein receptor from quecertin in inflammation mechanism and their optimization of the effectiveness in the human body. The bioinformatics tools used in this study are the database of quercetin compounds, Pubchem and Swis Target Prediction protein prediction databases, PyRx 0.8 molecular docking software, ligand docking, and binding site analysis with PyMOL and LigPlus software. The results from in silico show that quercetin compounds can interact with Muscleblind-like protein 1 target protein with a Binding Affinity minus value which is not much different from the dexamethasone compound. Dexamethason is a standart because it is a corticosteroid drug that can be used as an anti-inflammatory to reduce inflammation, allergic reactions, arthritis and other inflammatory diseases.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46295150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Leukocytes have an important role in driving away infections from pathogen microorganism by phagocytosis together with macrophages. The aim of this research was to analyze the leukocytes’ differentiation (eosinophil, basophil, neutrophil, lymphocytes, and monocytes) of mudskipper‘s blood (Periophthalmodon schlosseri ). Samples were taken from desa Tanipah Kalimantan Selatan. The sampling locations were decided by purposive or taking the data intentionally according to the consideration of mudskipper‘s location which is not homogenous. The data collection were conducted using Line Transect which means the sample was taken according to how many encounters can be taken. There were 15 mudskippers taken from Desa Tanipah used in this research. The parameter observed wasthe differentiation of leukocytes which consists of the percentages of monocytes, lymphocytes, eosinophil, basophil, and heterophil. The result showed that the percentage of lymphocytes in mudskippers is 62 + 4,1% and monocytes 24,7 + 0,8%, and the neutrophil is 0,5 + 0,1% and eosinophil 0,6 + 0,1% and no basophils were found. According to the result, it can be concluded that lymphocytesis the most dominant one amongst others.
{"title":"Leukocytes Description of Mudskipper (Periophthalmodon schlosseri) of Barito River Estuary, Desa Tanipah, Kalimantan Selatan","authors":"Heri Santoso, Hidayaturrahmah Hidayaturrahmah, Muhamat Muhamat","doi":"10.14421/BIOMEDICH.2018.71.1-4","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2018.71.1-4","url":null,"abstract":"Leukocytes have an important role in driving away infections from pathogen microorganism by phagocytosis together with macrophages. The aim of this research was to analyze the leukocytes’ differentiation (eosinophil, basophil, neutrophil, lymphocytes, and monocytes) of mudskipper‘s blood (Periophthalmodon schlosseri ). Samples were taken from desa Tanipah Kalimantan Selatan. The sampling locations were decided by purposive or taking the data intentionally according to the consideration of mudskipper‘s location which is not homogenous. The data collection were conducted using Line Transect which means the sample was taken according to how many encounters can be taken. There were 15 mudskippers taken from Desa Tanipah used in this research. The parameter observed wasthe differentiation of leukocytes which consists of the percentages of monocytes, lymphocytes, eosinophil, basophil, and heterophil. The result showed that the percentage of lymphocytes in mudskippers is 62 + 4,1% and monocytes 24,7 + 0,8%, and the neutrophil is 0,5 + 0,1% and eosinophil 0,6 + 0,1% and no basophils were found. According to the result, it can be concluded that lymphocytesis the most dominant one amongst others.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44578819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-30DOI: 10.14421/BIOMEDICH.2018.71.15-20
Namira Nur Arfa, B. Daryono, R. Reflinur
This study conducted in Laboratory of Molecular Biology, Balai Besar Bioteknologi dan Sumberdaya Genetik Pertanian (BB- Biogen) Bogor. The aims of this study are to determine and comparing the quantity, quality and the efficiency of DNA isolation result using detergent method and CTAB method. The parameters observed in this study are the value of DNA concentration, purity, and visualization result using gel electrophoresis. The samples are the leaves of Salak ‘Pondoh’ ( Salacca zalacca (Gaert.) Voss.). Detergent method is a method which was developed by Faculty of Biology UGM, it has simple method and relatively affordable cost. Meanwhile, CTAB method is one of the commonly used method s of DNA isolation protocol with relatively expensive cost. Detergent method used detergent in the cell wall separation and protein removal in the sample. The CTAB method used Cetyltrimethyl ammonium bromide (CTAB) for cell membrane separation in the sample. The research methods included DNA isolation with detergent and CTAB methods, PCR analysis and electrophoresis. Data analysis was done quantitatively using spectrophotometric method and qualitative used electrophoresis method. The result of the study showed that DNA isolation using CTAB method showed higher purity compared with detergent method with the purity values ranging from 1,3- 1,4 . Meanwhile, the concentration of DNA in the detergent method was higher than that of CTAB with the highest concentration of 1730 µg/ml. There is no difference between the quality of genomic DNA isolated by CTAB and detergent methods.
这项研究在分子生物学实验室进行，Balai Besar Bioteknologi dan Sumberdaya Genetik Pertanian（BB-Biogen）Bogor。本研究的目的是测定和比较洗涤剂法和CTAB法分离DNA的数量、质量和效率。本研究中观察到的参数是DNA浓度、纯度和凝胶电泳可视化结果的值。这些样品是萨拉克“Pondoh”（Salaca zalacca（Gaert.）Voss.）的叶子。清净剂法是由UGM生物学院开发的一种方法，方法简单，成本相对低廉。同时，CTAB法是DNA分离方案中常用的方法之一，成本相对较高。清净剂法采用清净剂对细胞壁进行分离，并去除样品中的蛋白质。CTAB法采用十六烷基三甲基溴化铵（CTAB）对样品进行细胞膜分离。研究方法包括用洗涤剂和CTAB法分离DNA、PCR分析和电泳。数据分析采用分光光度法定量，电泳法定性。研究结果表明，与洗涤剂法相比，CTAB法的DNA分离具有更高的纯度，其纯度值在1，3-1，4之间。同时，洗涤剂法中DNA的浓度高于CTAB，最高浓度为1730µg/ml。CTAB法和洗涤剂法分离的基因组DNA质量没有差异。
{"title":"Comparison of detergent and CTAB method for isolation of DNA from Salak ( Salacca zalacca (Gaert.) Voss. ‘Pondoh’)","authors":"Namira Nur Arfa, B. Daryono, R. Reflinur","doi":"10.14421/BIOMEDICH.2018.71.15-20","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2018.71.15-20","url":null,"abstract":"This study conducted in Laboratory of Molecular Biology, Balai Besar Bioteknologi dan Sumberdaya Genetik Pertanian (BB- Biogen) Bogor. The aims of this study are to determine and comparing the quantity, quality and the efficiency of DNA isolation result using detergent method and CTAB method. The parameters observed in this study are the value of DNA concentration, purity, and visualization result using gel electrophoresis. The samples are the leaves of Salak ‘Pondoh’ ( Salacca zalacca (Gaert.) Voss.). Detergent method is a method which was developed by Faculty of Biology UGM, it has simple method and relatively affordable cost. Meanwhile, CTAB method is one of the commonly used method s of DNA isolation protocol with relatively expensive cost. Detergent method used detergent in the cell wall separation and protein removal in the sample. The CTAB method used Cetyltrimethyl ammonium bromide (CTAB) for cell membrane separation in the sample. The research methods included DNA isolation with detergent and CTAB methods, PCR analysis and electrophoresis. Data analysis was done quantitatively using spectrophotometric method and qualitative used electrophoresis method. The result of the study showed that DNA isolation using CTAB method showed higher purity compared with detergent method with the purity values ranging from 1,3- 1,4 . Meanwhile, the concentration of DNA in the detergent method was higher than that of CTAB with the highest concentration of 1730 µg/ml. There is no difference between the quality of genomic DNA isolated by CTAB and detergent methods.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41474177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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