Pub Date : 2021-10-01DOI: 10.14421/BIOMEDICH.2021.102.81-86
Efe Sylvanus Abiya, F. Ologundudu, Ekpo wisdom
In Nigeria, like many developing nations, the resultant effect of land degradation: aggravated soil erosion, flood disasters, salinization or alkalisation, and the desertification have been a major public health concern for the past decades, however this study highlighted some of the factors that leads to the menace of soil fertility. The study was conducted at a gemstone mining site in Ijero Ekiti, Ekiti State, Nigeria. The soil samples were collected at a depth of 0-15 cm top soil and 0-30 cm subsoil. A line transect of 20 cm was drawn and soil sample was collected, all samples were kept in a clean container and labeled accordingly before been transported to the laboratory for analysis. The plant samples were thoroughly washed with distilled water to remove dust and other particles, air dried in a dust free wire meshed cage. All data obtained from this research were subjected to one-way analysis of variance (ANOVA). The result obtained in this study indicated that the levels of heavy metal concentration tested were still within the permissible limit in the root and shoot of Chromolaena odorata between the mine and control site. The implication of this is that Chromolaena odorata is safe for human and animal consumption. The said plant can readily undergo photosynthetic activity to aid growth by exploiting the presence of these metals either as a macro-nutrient or micro-nutrient as seen from the Translocation Factor and Metal Transfer Factor. The study concludes that soil at Ijero-Ekiti mine site were slightly acidic soil pH, reduced organic carbon, total nitrogen, available phosphorus, exchangeable cations and averagely elevated heavy metal contents.
{"title":"Risk Assessment of Heavy Metals in Chromolaena odorata Collected Around Gemstone Mining Site in Ijero-Ekiti","authors":"Efe Sylvanus Abiya, F. Ologundudu, Ekpo wisdom","doi":"10.14421/BIOMEDICH.2021.102.81-86","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2021.102.81-86","url":null,"abstract":"In Nigeria, like many developing nations, the resultant effect of land degradation: aggravated soil erosion, flood disasters, salinization or alkalisation, and the desertification have been a major public health concern for the past decades, however this study highlighted some of the factors that leads to the menace of soil fertility. The study was conducted at a gemstone mining site in Ijero Ekiti, Ekiti State, Nigeria. The soil samples were collected at a depth of 0-15 cm top soil and 0-30 cm subsoil. A line transect of 20 cm was drawn and soil sample was collected, all samples were kept in a clean container and labeled accordingly before been transported to the laboratory for analysis. The plant samples were thoroughly washed with distilled water to remove dust and other particles, air dried in a dust free wire meshed cage. All data obtained from this research were subjected to one-way analysis of variance (ANOVA). The result obtained in this study indicated that the levels of heavy metal concentration tested were still within the permissible limit in the root and shoot of Chromolaena odorata between the mine and control site. The implication of this is that Chromolaena odorata is safe for human and animal consumption. The said plant can readily undergo photosynthetic activity to aid growth by exploiting the presence of these metals either as a macro-nutrient or micro-nutrient as seen from the Translocation Factor and Metal Transfer Factor. The study concludes that soil at Ijero-Ekiti mine site were slightly acidic soil pH, reduced organic carbon, total nitrogen, available phosphorus, exchangeable cations and averagely elevated heavy metal contents.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45031881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.14421/BIOMEDICH.2021.102.73-79
David Ehikhuemen Okonofua, J. N. Asiwe, K. K. Anachuna, E. G. Moke, K. Sanusi, Ebunoluwa Oluwabusola Adagbada, Mariam Onono Yusuf, Damilola Ifeoluwa Alawode, A. Fasanmade
Diabetes mellitus is a common risk factor for erythrocyte osmotic stress. This study was aimed at exploring the effect of streptozotocin (STZ)-induced diabetes mellitus and salt-induced hypertension on osmotic fragility and hemorheological variables in male Wistar rats. Thirty male rats were grouped into five groups of six animals each as follows: negative control (zero salt in diet); positive control (normal salt diet - 0.3% salt); high salt diet (8% salt) (HSD only); STZ induced diabetes and normal salt diet (STZ only); STZ induced diabetes and high salt diet (STZ + HSD). At the end of a 4 weeks period, hematological variables, osmotic fragility, rheology and cardiovascular responses were assessed. There was an increase (p<0.05) in the mean arterial pressure and heart rate of HSD, STZ and HSD + STZ groups indicating a salt induced hypertension. There was a decrease in the body weight of STZ and HSD +STZ groups. There was significant increase (p<0.05) in the haematocrit, platelets estimates and fibrinogen concentrations in the experimental groups when compared with the controls. The STZ and STZ + HSD groups showed a reduced clotting time which corresponded to the increased platelet estimates and fibrinogen concentration. The increase in haematocrit, platelet and plasma protein resulted in the increased blood viscosity and a decreased flow rate. The osmotic fragility test was also observed to be increased (p<0.05) in HSD, STZ only and STZ + HSD groups. Diabetes mellitus and hypertension increase the rate of hemolysis of erythrocyte, as well as increase blood viscosity.
{"title":"Effect of Diabetes Mellitus and Hypertension on Osmotic Fragility and Hemorheological Factors in Male Wistar Rats","authors":"David Ehikhuemen Okonofua, J. N. Asiwe, K. K. Anachuna, E. G. Moke, K. Sanusi, Ebunoluwa Oluwabusola Adagbada, Mariam Onono Yusuf, Damilola Ifeoluwa Alawode, A. Fasanmade","doi":"10.14421/BIOMEDICH.2021.102.73-79","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2021.102.73-79","url":null,"abstract":"Diabetes mellitus is a common risk factor for erythrocyte osmotic stress. This study was aimed at exploring the effect of streptozotocin (STZ)-induced diabetes mellitus and salt-induced hypertension on osmotic fragility and hemorheological variables in male Wistar rats. Thirty male rats were grouped into five groups of six animals each as follows: negative control (zero salt in diet); positive control (normal salt diet - 0.3% salt); high salt diet (8% salt) (HSD only); STZ induced diabetes and normal salt diet (STZ only); STZ induced diabetes and high salt diet (STZ + HSD). At the end of a 4 weeks period, hematological variables, osmotic fragility, rheology and cardiovascular responses were assessed. There was an increase (p<0.05) in the mean arterial pressure and heart rate of HSD, STZ and HSD + STZ groups indicating a salt induced hypertension. There was a decrease in the body weight of STZ and HSD +STZ groups. There was significant increase (p<0.05) in the haematocrit, platelets estimates and fibrinogen concentrations in the experimental groups when compared with the controls. The STZ and STZ + HSD groups showed a reduced clotting time which corresponded to the increased platelet estimates and fibrinogen concentration. The increase in haematocrit, platelet and plasma protein resulted in the increased blood viscosity and a decreased flow rate. The osmotic fragility test was also observed to be increased (p<0.05) in HSD, STZ only and STZ + HSD groups. Diabetes mellitus and hypertension increase the rate of hemolysis of erythrocyte, as well as increase blood viscosity.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87049200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-29DOI: 10.14421/BIOMEDICH.2021.101.67-71
E. G. Moke, E. K. Umukoro, E. Ojugbeli, T. Ezedom, T.M.E. Daubry, Iziegbe Lisa Omorodion
Medicinal plants usage in traditional medicine has risen over the years. Musa paradisiaca has been reported that it possesses various therapeutic efficacies. This study is aimed at evaluating the effect of parts of the ethanol fruit extracts of Musa paradisiaca on haematological indices and serum liver enzymes. Thirty Wistar rats were grouped into five groups of six animals each which were administered 200 mg/kg and 400 mg/kg of Musa paradisiac fruit (MPF) pulp extract or peel extract. Haematological indices and liver enzymes were assayed for at the end of the 14-days experimental period. MPF pulp and MPF peel at 400 mg/kg showed a significant (P<0.05) increase in red cell count and haematocrit level as compared to the control. There was a non-significant (P>0.05) increase in haemoglobin concentration in the treated rats as compared to the control. There was also a non-significant (P>0.05) change in AST, ALT, and ALP level of the treated rats as compared to the control. Fruit pulp and peel of Musa paradisiaca improve erythrocytes count and haematocrit level, and they may not be associated with liver toxicity.
{"title":"Effect of Ethanol Extracts of Musa paradisiaca Fruit Pulp and Peels on Haematological Indices and Liver Enzymes of Experimental Rats","authors":"E. G. Moke, E. K. Umukoro, E. Ojugbeli, T. Ezedom, T.M.E. Daubry, Iziegbe Lisa Omorodion","doi":"10.14421/BIOMEDICH.2021.101.67-71","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2021.101.67-71","url":null,"abstract":"Medicinal plants usage in traditional medicine has risen over the years. Musa paradisiaca has been reported that it possesses various therapeutic efficacies. This study is aimed at evaluating the effect of parts of the ethanol fruit extracts of Musa paradisiaca on haematological indices and serum liver enzymes. Thirty Wistar rats were grouped into five groups of six animals each which were administered 200 mg/kg and 400 mg/kg of Musa paradisiac fruit (MPF) pulp extract or peel extract. Haematological indices and liver enzymes were assayed for at the end of the 14-days experimental period. MPF pulp and MPF peel at 400 mg/kg showed a significant (P<0.05) increase in red cell count and haematocrit level as compared to the control. There was a non-significant (P>0.05) increase in haemoglobin concentration in the treated rats as compared to the control. There was also a non-significant (P>0.05) change in AST, ALT, and ALP level of the treated rats as compared to the control. Fruit pulp and peel of Musa paradisiaca improve erythrocytes count and haematocrit level, and they may not be associated with liver toxicity.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75076799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-27DOI: 10.14421/BIOMEDICH.2021.101.59-65
Subhashinie Sanadheera, D. Subasinghe, M. Solangaarachchi, M. Suraweera, N. Suraweera, Nadeesha Tharangika
According to traditional medicine, Hibiscus is used to treat hypertension, heart diseases and many other ailments. Commercially available Hibiscus tea is prepared with H. sabdariffa and is expensive. H. rosasinensis is the common variety which is abundant in tropical home-gardens, however the scientific data are lacking on the effects. The present review focuses on the available scientific data on the effects of H. rosasinensis on controlling diabetes and hypercholesterolemia. The endeavour was to identify whether H. rosasinensis flower petals as a ‘tea’ is effective for diabetic and hypercholesterolemic patients. Medical databases such as MEDLINE, BMC, BMJ, research gate, Mendelay literature search database, Google scholar and the general engine Google were searched from November 2018 to April 2019. Search was carried out using keywords such as “Hibiscus rosasinensis”, “antidiabetic effects”, “antihyperlipidemic effects”, “toxic effects”, “anti-inflammatory effects”, “phytochemicals in Hibiscus” etc. Data were critically analyzed to assess whether the effective doses of the research studies on a par with the doses present in H. rosasinensis teas. We found thirty-nine journal articles fulfilled the criteria. The data were categorized and extracted on uses of H. rosasinensis, anti-diabetic effects, anti-inflammatory effects, anti-hypercholesterolemic effects and its phytochemicals. The review revealed that the dose of H. rosasinensis petals present in a home-made Hibiscus tea is theoretically sufficient to elicit anti-hyperglycemic and anti-hyperlipidemic effects. Home-made Hibiscus tea is effective in controlling diabetes and hypercholesterolemia without causing acute toxicity.
{"title":"Hibiscus rosa-sinensis L. (red Hibiscus) Tea, Can It Be Used as A Home-Remedy to Control Diabetes and Hypercholesterolemia?","authors":"Subhashinie Sanadheera, D. Subasinghe, M. Solangaarachchi, M. Suraweera, N. Suraweera, Nadeesha Tharangika","doi":"10.14421/BIOMEDICH.2021.101.59-65","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2021.101.59-65","url":null,"abstract":"According to traditional medicine, Hibiscus is used to treat hypertension, heart diseases and many other ailments. Commercially available Hibiscus tea is prepared with H. sabdariffa and is expensive. H. rosasinensis is the common variety which is abundant in tropical home-gardens, however the scientific data are lacking on the effects. The present review focuses on the available scientific data on the effects of H. rosasinensis on controlling diabetes and hypercholesterolemia. The endeavour was to identify whether H. rosasinensis flower petals as a ‘tea’ is effective for diabetic and hypercholesterolemic patients. Medical databases such as MEDLINE, BMC, BMJ, research gate, Mendelay literature search database, Google scholar and the general engine Google were searched from November 2018 to April 2019. Search was carried out using keywords such as “Hibiscus rosasinensis”, “antidiabetic effects”, “antihyperlipidemic effects”, “toxic effects”, “anti-inflammatory effects”, “phytochemicals in Hibiscus” etc. Data were critically analyzed to assess whether the effective doses of the research studies on a par with the doses present in H. rosasinensis teas. We found thirty-nine journal articles fulfilled the criteria. The data were categorized and extracted on uses of H. rosasinensis, anti-diabetic effects, anti-inflammatory effects, anti-hypercholesterolemic effects and its phytochemicals. The review revealed that the dose of H. rosasinensis petals present in a home-made Hibiscus tea is theoretically sufficient to elicit anti-hyperglycemic and anti-hyperlipidemic effects. Home-made Hibiscus tea is effective in controlling diabetes and hypercholesterolemia without causing acute toxicity.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89954855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-19DOI: 10.14421/BIOMEDICH.2021.101.33-39
Lita Ayu Umartani, M. S. Nahdi
Ethnobotany is a study of the interaction between local people and their natural environment, especially regarding the use of plants as food and medicinal ingredients. Edible plants are a daily basic need whose existence is a necessity for the people on the slopes of Mount Merapi and Merbabu. How to use plants is transferred from generation to generation to form a culture. The research was carried out in March-May 2020 with the aim of digging local knowledge about plants used as food, including staple food, vegetables and medicines by studying the species diversity, including the benefits of plant parts, habit, how to use, process and how to obtain these species. The data were collected by using a combination of qualitative and quantitative methods with in-depth interviews through 40 respondents who were selected by purposive sampling. The results showed that the communities on the slopes of Mount Merapi and Merbabu used 74 plant species from 37 families as food sources. The favorite family is Fabaceae, followed by Zingiberaceae and Solanaceae. The most widely used habitus of plants were herbs (36.49%), followed by bush (28.38%), shrubs (18.92%) and trees (16.22%). Plant parts that are widely used are leaves (29.73%), fruit (17.57%), tubers (10.81%), seeds (9.46%), roots, rhizomes and flowers (6.76%), shoots (5.40%), stems (2.70%) and water, skin and heartwood (1.35%). The most common ways to use it are eaten raw as vegetables (29.73%), boiled (16.22%) and drink (12.16%). How to obtain it are through own cultivation (72.97%), and buying at the market (21.62%). The highest use values were Adas (Foeniculum vulgare Mill.) (0.25), chili pepper (Capsicum annum L.) (0.20), turmeric (Curcuma dosmetica loir) and water spinach (Ipomoea aquatica Forsk.) (0.17). The highest importance values were rice (Oryza sativa L.) (5.23%), and fennel (Foeniculum vulgare Mill.) (4.57%).
{"title":"Ethnobotanical Study of Edible Plant Communities on the Slopes of Mount Merapi and Merbabu, Indonesia","authors":"Lita Ayu Umartani, M. S. Nahdi","doi":"10.14421/BIOMEDICH.2021.101.33-39","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2021.101.33-39","url":null,"abstract":"Ethnobotany is a study of the interaction between local people and their natural environment, especially regarding the use of plants as food and medicinal ingredients. Edible plants are a daily basic need whose existence is a necessity for the people on the slopes of Mount Merapi and Merbabu. How to use plants is transferred from generation to generation to form a culture. The research was carried out in March-May 2020 with the aim of digging local knowledge about plants used as food, including staple food, vegetables and medicines by studying the species diversity, including the benefits of plant parts, habit, how to use, process and how to obtain these species. The data were collected by using a combination of qualitative and quantitative methods with in-depth interviews through 40 respondents who were selected by purposive sampling. The results showed that the communities on the slopes of Mount Merapi and Merbabu used 74 plant species from 37 families as food sources. The favorite family is Fabaceae, followed by Zingiberaceae and Solanaceae. The most widely used habitus of plants were herbs (36.49%), followed by bush (28.38%), shrubs (18.92%) and trees (16.22%). Plant parts that are widely used are leaves (29.73%), fruit (17.57%), tubers (10.81%), seeds (9.46%), roots, rhizomes and flowers (6.76%), shoots (5.40%), stems (2.70%) and water, skin and heartwood (1.35%). The most common ways to use it are eaten raw as vegetables (29.73%), boiled (16.22%) and drink (12.16%). How to obtain it are through own cultivation (72.97%), and buying at the market (21.62%). The highest use values were Adas (Foeniculum vulgare Mill.) (0.25), chili pepper (Capsicum annum L.) (0.20), turmeric (Curcuma dosmetica loir) and water spinach (Ipomoea aquatica Forsk.) (0.17). The highest importance values were rice (Oryza sativa L.) (5.23%), and fennel (Foeniculum vulgare Mill.) (4.57%).","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91023971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-19DOI: 10.14421/BIOMEDICH.2021.101.41-46
D. Krisdiyanto, Sudarlin Sudarlin, Hikmah Supriyati
The electronic transition processes of α-mangostin and β-mangostin as dye compounds in DSSC and their ability to transfer electrons to semiconductors have been studied in theory. The research was carried out computationally using the NwChem application. The methods used are Density Functional Theory (DFT) for structure optimization and Time-Dependent DFT (TD-DFT) for electronic transitions. The results showed that the energy of HOMO β-mangostin was lower that it was more stable or easier to form bonds with semiconductors. Likewise, its LUMO energy is lower than α-mangostin that it is easier to inject electrons into the semiconductor. The energy difference of HOMO-LUMO β-mangostin is smaller than α-mangostin. This shows that β-mangostin is more easily excited or more efficient in exciting solar energy to convert it into electricity. This is consistent with the molecular spectra where λmaxβ-mangostin is at a higher wavelength. Orbital analysis shows electron injection of α-mangostin and β-mangostin into the semiconductor via double O atoms in each molecule. The injection is influenced by the bond length, where the bond length of α-mangostin to TiO2 is smaller than that of β-mangostin to TiO2.
{"title":"Computational Chemical Study of Pigment of Mangosteen (Garcinia mangostana) Rind Extract as Dye Compound in Dye-Sensitized Solar Cell (DSSC)","authors":"D. Krisdiyanto, Sudarlin Sudarlin, Hikmah Supriyati","doi":"10.14421/BIOMEDICH.2021.101.41-46","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2021.101.41-46","url":null,"abstract":"The electronic transition processes of α-mangostin and β-mangostin as dye compounds in DSSC and their ability to transfer electrons to semiconductors have been studied in theory. The research was carried out computationally using the NwChem application. The methods used are Density Functional Theory (DFT) for structure optimization and Time-Dependent DFT (TD-DFT) for electronic transitions. The results showed that the energy of HOMO β-mangostin was lower that it was more stable or easier to form bonds with semiconductors. Likewise, its LUMO energy is lower than α-mangostin that it is easier to inject electrons into the semiconductor. The energy difference of HOMO-LUMO β-mangostin is smaller than α-mangostin. This shows that β-mangostin is more easily excited or more efficient in exciting solar energy to convert it into electricity. This is consistent with the molecular spectra where λmaxβ-mangostin is at a higher wavelength. Orbital analysis shows electron injection of α-mangostin and β-mangostin into the semiconductor via double O atoms in each molecule. The injection is influenced by the bond length, where the bond length of α-mangostin to TiO2 is smaller than that of β-mangostin to TiO2.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81761262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-16DOI: 10.14421/BIOMEDICH.2021.101.27-32
Z. Mohammedi
Honey and propolis is natural food, produced by honey bees (Apis mellifera) and largely used by the local population for its medicinal properties. Our work aims to extract and evaluate the hydrogen peroxide scavenging capacity of different phenolic extracts from some bee products. Phenolic compounds from honey samples, propolis, and bee pollen were extracted by methanol and subjected to radical scavenging activity towards hydrogen peroxide. The results showed the highest values for the total phenolic and total flavonoïd contents in propolis and bee pollen, and a great hydrogen peroxide (H2O2) inhibition (IC50: 0.205 - 2.210 µg/mL) with honey extracts, while sample “multiflower” is the better antioxidant, more than ascorbic acid used as control. The less scavenging activity was observed with the extract from bee pollen (IC50: 39.383 µg/mL). To combat the harmful effects of free radicals, especially reactive oxygen species including hydrogen peroxide, it is important to use phenolic extracts instead of using honey as it is, and extracts from the honey of different types are excellent antioxidants compared to other bee products.
{"title":"Extraction, Phenolic Content and Hydrogen Peroxide Scavenging Capacity of Extracts from Some Honey Samples, Propolis and Bee Pollen","authors":"Z. Mohammedi","doi":"10.14421/BIOMEDICH.2021.101.27-32","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2021.101.27-32","url":null,"abstract":"Honey and propolis is natural food, produced by honey bees (Apis mellifera) and largely used by the local population for its medicinal properties. Our work aims to extract and evaluate the hydrogen peroxide scavenging capacity of different phenolic extracts from some bee products. Phenolic compounds from honey samples, propolis, and bee pollen were extracted by methanol and subjected to radical scavenging activity towards hydrogen peroxide. The results showed the highest values for the total phenolic and total flavonoïd contents in propolis and bee pollen, and a great hydrogen peroxide (H2O2) inhibition (IC50: 0.205 - 2.210 µg/mL) with honey extracts, while sample “multiflower” is the better antioxidant, more than ascorbic acid used as control. The less scavenging activity was observed with the extract from bee pollen (IC50: 39.383 µg/mL). To combat the harmful effects of free radicals, especially reactive oxygen species including hydrogen peroxide, it is important to use phenolic extracts instead of using honey as it is, and extracts from the honey of different types are excellent antioxidants compared to other bee products.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86219972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-13DOI: 10.14421/BIOMEDICH.2021.101.23-25
Marsha Darmawan, Elysanti Dwi Maharani
Osteogenesis Imperfecta is a hereditary connective tissue disorder due to COL1A1/2 mutation causing gene defect encoding proteins to metabolize collagen. The skeletal manifestation of OI causing bone incompetence, hence the name brittle bone disease. Here we report three cases of OI type IV in adults. Skeletal conventional X-rays were performed to all patients and all of them has similar results such as bowing deformities of long bones, old union and some non-union fractures with extreme angulation and severe osteoporosis. OI are classified based on skeletal structure, sclera colorization, dentinogenesis, and functional metabolic defect genetically. OI type I and IV can live until adults; also, the same type of OI can be found in siblings. Skeletal conventional X-rays can solely make the diagnosis.
{"title":"Brittle Bone Brothers: Osteogenesis Imperfecta Conventional Serial Case","authors":"Marsha Darmawan, Elysanti Dwi Maharani","doi":"10.14421/BIOMEDICH.2021.101.23-25","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2021.101.23-25","url":null,"abstract":"Osteogenesis Imperfecta is a hereditary connective tissue disorder due to COL1A1/2 mutation causing gene defect encoding proteins to metabolize collagen. The skeletal manifestation of OI causing bone incompetence, hence the name brittle bone disease. Here we report three cases of OI type IV in adults. Skeletal conventional X-rays were performed to all patients and all of them has similar results such as bowing deformities of long bones, old union and some non-union fractures with extreme angulation and severe osteoporosis. OI are classified based on skeletal structure, sclera colorization, dentinogenesis, and functional metabolic defect genetically. OI type I and IV can live until adults; also, the same type of OI can be found in siblings. Skeletal conventional X-rays can solely make the diagnosis.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76039441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-01DOI: 10.14421/BIOMEDICH.2021.101.15-21
Kelemu Mulluye, A. Kebede, N. Bussa
Pectinases are the group of enzymes that degrade pectin. This study was conducted with the aim of isolation of efficient pectinase producing pectinolytic fungi from the decomposing mango peels using extracted mango peels pectin as a growth substrate under submerged fermentation, determining optimum pectinase production conditions with regards to some physicochemical parameters. The organisms were screened for the production of pectinase using Pectin agar media, and the two active pectinolytic fungi (P1 and P2) were isolated. pectinase production media was later used for the Lab scale production of pectinase by inoculating p1 and p2 and incubating for 7 days. The enzyme was extracted after seven days of fermentation and every day tested for their pectinolytic activity. P2 showed relatively higher pectinolytic activity and was therefore used for further studies. P2 was inoculated into a broth containing mango pectin under submerged fermentation. Results indicate that a pectin yield of mango peel 17.75%. Different parameters optimization processes were investigated on submerged fermentation namely pH, incubation period, temperature and substrate concentration optima were found 6, 4 days, 35oC and 1.5% respectively. The result suggests that mango peels have high pectin content and can be used for the value-added synthesis of pectinase.
{"title":"Production and Optimization of Pectinase from Pectinolytic Fungi Cultivated on Mango peels and Pectin Subjected to Submerged Fermentation","authors":"Kelemu Mulluye, A. Kebede, N. Bussa","doi":"10.14421/BIOMEDICH.2021.101.15-21","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2021.101.15-21","url":null,"abstract":"Pectinases are the group of enzymes that degrade pectin. This study was conducted with the aim of isolation of efficient pectinase producing pectinolytic fungi from the decomposing mango peels using extracted mango peels pectin as a growth substrate under submerged fermentation, determining optimum pectinase production conditions with regards to some physicochemical parameters. The organisms were screened for the production of pectinase using Pectin agar media, and the two active pectinolytic fungi (P1 and P2) were isolated. pectinase production media was later used for the Lab scale production of pectinase by inoculating p1 and p2 and incubating for 7 days. The enzyme was extracted after seven days of fermentation and every day tested for their pectinolytic activity. P2 showed relatively higher pectinolytic activity and was therefore used for further studies. P2 was inoculated into a broth containing mango pectin under submerged fermentation. Results indicate that a pectin yield of mango peel 17.75%. Different parameters optimization processes were investigated on submerged fermentation namely pH, incubation period, temperature and substrate concentration optima were found 6, 4 days, 35oC and 1.5% respectively. The result suggests that mango peels have high pectin content and can be used for the value-added synthesis of pectinase.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79783261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-01DOI: 10.14421/BIOMEDICH.2021.101.1-5
M. Y. Hardiansyah, Y. Musa, A. M. Jaya
The low productivity of cocoa plantations in Indonesia is partly due to the low quality of seeds, which refers to the impeded growth of cultivated cocoa nurseries. Seed is the initial growth of plants so the importance of giving special treatment to seeds will refer to better seed growth. Provision of Plant Growth Promoting Rhizobacteria (PGPR) microbes can produce indoleacetic acid (IAA) in plants to improve the quality of plant growth. This study aims to determine the effectiveness of the provision of Plant Growth Promoting Rhizobacteria bamboo rhizosphere against cocoa seed germination. The study was carried out in the farmer group garden, Gantarangkeke District, Bantaeng. This study was arranged in the form of a two-factor factorial design (F2F) in a randomized block design (RBD). The use of cocoa seed type as the first factor consisted of GTB (Gantarangkeke Bantaeng) local cocoa seed and MCC 01 cocoa seed and seed immersion treatment at PGPR rhizosphere bamboo concentration as the second factor consisting of 0% (control) concentration, 5%, 10 % and 15%. The results obtained indicate that administration of seeds with bamboo rhizosphere PGPR affects the germination (100.00%), the speed of seed growth (7.14%/etmal), as well as on abnormal seeds (10.00%). So that the provision of bamboo rhizosphere PGPR on cocoa seeds has an effective influence on seed germination and cocoa seedling development.
{"title":"The Effectiveness of Giving Plant PGPR Rhizosphere Bamboo on Cocoa Seeds Germination at The Nursery Level","authors":"M. Y. Hardiansyah, Y. Musa, A. M. Jaya","doi":"10.14421/BIOMEDICH.2021.101.1-5","DOIUrl":"https://doi.org/10.14421/BIOMEDICH.2021.101.1-5","url":null,"abstract":"The low productivity of cocoa plantations in Indonesia is partly due to the low quality of seeds, which refers to the impeded growth of cultivated cocoa nurseries. Seed is the initial growth of plants so the importance of giving special treatment to seeds will refer to better seed growth. Provision of Plant Growth Promoting Rhizobacteria (PGPR) microbes can produce indoleacetic acid (IAA) in plants to improve the quality of plant growth. This study aims to determine the effectiveness of the provision of Plant Growth Promoting Rhizobacteria bamboo rhizosphere against cocoa seed germination. The study was carried out in the farmer group garden, Gantarangkeke District, Bantaeng. This study was arranged in the form of a two-factor factorial design (F2F) in a randomized block design (RBD). The use of cocoa seed type as the first factor consisted of GTB (Gantarangkeke Bantaeng) local cocoa seed and MCC 01 cocoa seed and seed immersion treatment at PGPR rhizosphere bamboo concentration as the second factor consisting of 0% (control) concentration, 5%, 10 % and 15%. The results obtained indicate that administration of seeds with bamboo rhizosphere PGPR affects the germination (100.00%), the speed of seed growth (7.14%/etmal), as well as on abnormal seeds (10.00%). So that the provision of bamboo rhizosphere PGPR on cocoa seeds has an effective influence on seed germination and cocoa seedling development.","PeriodicalId":8882,"journal":{"name":"Biology, Medicine, & Natural Product Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79541227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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