Biotechnology advances最新文献_第7页

Innovative strategies to enhance lipid accumulation for algal biodiesel production: Ultrasound treatment, external electrostimulation, and integration of bioelectrochemical systems 提高藻类生物柴油生产中脂质积累的创新策略：超声处理、外部电刺激和生物电化学系统的集成。

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-10-17 DOI: 10.1016/j.biotechadv.2025.108740

Iryna Rusyn

Microalgae-based biodiesel, known as third-generation biodiesel, offers a promising path forward by combining efficient carbon capture with high biofuel yields. At the same time, it can address ethical and environmental concerns related to competition with food crops and habitat loss caused by oil crop cultivation, which remain critical challenges for sustainable biodiesel development. However, despite its potential, algal biodiesel production is hampered by several critical limitations, particularly the inherent trade-off between biomass accumulation and lipid synthesis, the low biomass and lipid yields, and the high costs associated with cultivation technologies and downstream processing. This study provides an in-depth review of recent advances in innovative ultrasonic and electrochemical strategies to overcome these bottlenecks into algal cultivation processes. Furthermore, our discussions have covered underlying mechanisms through which these stimuli affect algal metabolism, with the goal of gaining deeper insight into their applicability and long-term potential in sustainable biofuel production systems. The findings of this study demonstrate that ultrasound treatment, external electrostimulation, and the integration of bioelectrochemical systems represent effective tools markedly enhance microalgal biomass productivity and lipid accumulation. Optimization of treatment regimes and their synergistic interaction with other factors provides a robust approach to concurrently stimulating both algal growth and lipid biosynthesis. Additionally, these approaches also streamline downstream processing by improving lipid extraction efficiency, reducing reaction time, minimizing chemical input, and decreasing the number of processing stages. The compatibility of these technologies with waste-based cultivation and remediation further enhances their sustainability profile while simultaneously reducing operational costs on aeration demand, and CO₂ supplementation requirements. Representing promising frontiers in the field, these emerging and insufficiently studied approaches offer both environmental and economic advantages and warrant intensified research efforts to fully unlock their potential for advancing sustainable algal biofuel production.

以微藻为基础的生物柴油，被称为第三代生物柴油，通过将高效的碳捕获与高生物燃料产量相结合，提供了一条有前途的发展道路。与此同时，它可以解决与粮食作物竞争和油料作物种植造成的栖息地丧失有关的伦理和环境问题，这些问题仍然是可持续生物柴油发展的关键挑战。然而，尽管有潜力，藻类生物柴油的生产受到几个关键限制的阻碍，特别是生物质积累和脂质合成之间的内在权衡，低生物量和脂质产量，以及与培养技术和下游加工相关的高成本。本研究深入回顾了近年来在创新超声和电化学策略方面的进展，以克服藻类培养过程中的这些瓶颈。此外，我们的讨论涵盖了这些刺激影响藻类代谢的潜在机制，目的是更深入地了解它们在可持续生物燃料生产系统中的适用性和长期潜力。本研究结果表明，超声处理、外部电刺激和生物电化学系统的整合是有效的工具，可显著提高微藻生物量生产力和脂质积累。优化处理方案及其与其他因素的协同作用为同时刺激藻类生长和脂质生物合成提供了一种强有力的方法。此外，这些方法还通过提高脂质提取效率、缩短反应时间、减少化学投入和减少处理阶段数量来简化下游加工。这些技术与基于废物的种植和修复的兼容性进一步提高了其可持续性，同时降低了曝气需求和CO₂补充需求的运营成本。这些新兴的、尚未得到充分研究的方法代表了该领域前景广阔的前沿，提供了环境和经济优势，需要加强研究工作，以充分释放其推进可持续藻类生物燃料生产的潜力。

{"title":"Innovative strategies to enhance lipid accumulation for algal biodiesel production: Ultrasound treatment, external electrostimulation, and integration of bioelectrochemical systems","authors":"Iryna Rusyn","doi":"10.1016/j.biotechadv.2025.108740","DOIUrl":"10.1016/j.biotechadv.2025.108740","url":null,"abstract":"<div><div>Microalgae-based biodiesel, known as third-generation biodiesel, offers a promising path forward by combining efficient carbon capture with high biofuel yields. At the same time, it can address ethical and environmental concerns related to competition with food crops and habitat loss caused by oil crop cultivation, which remain critical challenges for sustainable biodiesel development. However, despite its potential, algal biodiesel production is hampered by several critical limitations, particularly the inherent trade-off between biomass accumulation and lipid synthesis, the low biomass and lipid yields, and the high costs associated with cultivation technologies and downstream processing. This study provides an in-depth review of recent advances in innovative ultrasonic and electrochemical strategies to overcome these bottlenecks into algal cultivation processes. Furthermore, our discussions have covered underlying mechanisms through which these stimuli affect algal metabolism, with the goal of gaining deeper insight into their applicability and long-term potential in sustainable biofuel production systems. The findings of this study demonstrate that ultrasound treatment, external electrostimulation, and the integration of bioelectrochemical systems represent effective tools markedly enhance microalgal biomass productivity and lipid accumulation. Optimization of treatment regimes and their synergistic interaction with other factors provides a robust approach to concurrently stimulating both algal growth and lipid biosynthesis. Additionally, these approaches also streamline downstream processing by improving lipid extraction efficiency, reducing reaction time, minimizing chemical input, and decreasing the number of processing stages. The compatibility of these technologies with waste-based cultivation and remediation further enhances their sustainability profile while simultaneously reducing operational costs on aeration demand, and CO₂ supplementation requirements. Representing promising frontiers in the field, these emerging and insufficiently studied approaches offer both environmental and economic advantages and warrant intensified research efforts to fully unlock their potential for advancing sustainable algal biofuel production.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"86 ","pages":"Article 108740"},"PeriodicalIF":12.5,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145328521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Material-binding peptides: sources, mechanisms, directed evolution and applications 物质结合肽：来源、机制、定向进化和应用。

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-10-16 DOI: 10.1016/j.biotechadv.2025.108742

Huan Chen , Ya-Ting Gao , Xu-Zhe Ge , Xin Wang , Feng Cheng , Ya-Ping Xue , Yu-Guo Zheng

Material-binding peptides (MBPs) can specifically bind to materials under mild conditions, such as room temperature and aqueous environments, thereby offering promising applications in both biotechnology and materials science. Recent advances in screening techniques, including phage display, bacterial display, and proteomics-based methods, combined with innovations in protein engineering and machine learning, have significantly accelerated the discovery and optimization of MBPs. These peptides have been successfully applied in areas such as catalyst immobilization (biocatalysis), biodegradation, and biomimetic mineralization. This review provides a comprehensive synthesis of the state-of-the-art in MBP research. It begins by discussing the sources of MBPs and the engineering strategies used to enhance their performance, then delves into the molecular mechanisms underlying their material interactions, and finally examines their emerging industrial applications. The review aims to guide researchers through current screening methodologies, provide mechanistic insights, and explore practical applications, offering a roadmap for future advancements in the field.

材料结合肽（MBPs）可以在室温和水环境等温和条件下特异性结合材料，因此在生物技术和材料科学中都有很好的应用前景。筛选技术的最新进展，包括噬菌体展示、细菌展示和基于蛋白质组学的方法，结合蛋白质工程和机器学习的创新，极大地加速了MBPs的发现和优化。这些多肽已成功应用于催化剂固定化（生物催化）、生物降解和仿生矿化等领域。本文综述了MBP研究的最新进展。首先讨论MBPs的来源和用于提高其性能的工程策略，然后深入研究其材料相互作用的分子机制，最后研究其新兴的工业应用。该综述旨在指导研究人员了解当前的筛选方法，提供机制见解，并探索实际应用，为该领域的未来发展提供路线图。

{"title":"Material-binding peptides: sources, mechanisms, directed evolution and applications","authors":"Huan Chen , Ya-Ting Gao , Xu-Zhe Ge , Xin Wang , Feng Cheng , Ya-Ping Xue , Yu-Guo Zheng","doi":"10.1016/j.biotechadv.2025.108742","DOIUrl":"10.1016/j.biotechadv.2025.108742","url":null,"abstract":"<div><div>Material-binding peptides (MBPs) can specifically bind to materials under mild conditions, such as room temperature and aqueous environments, thereby offering promising applications in both biotechnology and materials science. Recent advances in screening techniques, including phage display, bacterial display, and proteomics-based methods, combined with innovations in protein engineering and machine learning, have significantly accelerated the discovery and optimization of MBPs. These peptides have been successfully applied in areas such as catalyst immobilization (biocatalysis), biodegradation, and biomimetic mineralization. This review provides a comprehensive synthesis of the state-of-the-art in MBP research. It begins by discussing the sources of MBPs and the engineering strategies used to enhance their performance, then delves into the molecular mechanisms underlying their material interactions, and finally examines their emerging industrial applications. The review aims to guide researchers through current screening methodologies, provide mechanistic insights, and explore practical applications, offering a roadmap for future advancements in the field.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"86 ","pages":"Article 108742"},"PeriodicalIF":12.5,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smart culture medium optimization for recombinant protein production: Experimental, modeling, and AI/ML-driven strategies 重组蛋白生产的智能培养基优化：实验、建模和AI/ ml驱动策略。

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-10-15 DOI: 10.1016/j.biotechadv.2025.108738

Galib Khan , Carrie Sanford , Cong T. Trinh

Recombinant protein production (RPP) is central to biotechnology, where recombinant proteins are used as either end products or catalysts in the synthesis of chemicals, fuels, and materials. Among the major cost drivers, culture medium plays a pivotal role in determining protein yield and quality. This review presents a comprehensive perspective on the critical stages of “smart” culture medium optimization: planning, screening, modeling, optimization, and validation. In the planning stage, we examine the nutritional and energetic roles of medium components, including carbon, nitrogen, amino acids, salts, and trace metals, and their impacts on culture parameters such as pH, oxidative state, and osmolality. We highlight the variability in trace metal content due to water sources, culture vessels, and raw materials, which can substantially influence RPP. The screening stage covers Design of Experiments (DoE) approaches, assessing their theoretical basis, implementation, and limitations. For modeling, we describe methods that integrate experimental data to develop predictive models for smart medium formulation. Model-based optimization strategies can then be employed to select optimal media compositions for a given application. The validation stage aims to evaluate model predictions and provide feedback for model training and refinement. Finally, we survey mechanistic and artificial intelligence/machine learning (AI/ML)-driven models as integrated, transformational tools for predictive modeling of bioprocess conditions, nutrient availability, cellular metabolism, and protein quality, with the goal of optimizing culture media to enhance protein yields while reducing costs and environmental impact. We conclude by addressing the challenges of translating laboratory-scale medium optimization to industrial-scale settings and exploring future AI/ML-driven approaches that may overcome current bottlenecks and accelerate medium design for RPP. Overall, this review provides a unified framework for advancing smart medium design in RPP.

重组蛋白生产（RPP）是生物技术的核心，重组蛋白被用作合成化学品、燃料和材料的最终产品或催化剂。在主要的成本驱动因素中，培养基在决定蛋白质产量和质量方面起着关键作用。本文综述了“智能”培养基优化的关键阶段：规划、筛选、建模、优化和验证。在计划阶段，我们研究了培养基成分的营养和能量作用，包括碳、氮、氨基酸、盐和微量金属，以及它们对pH、氧化状态和渗透压等培养参数的影响。我们强调了由于水源、培养容器和原材料而引起的微量金属含量的变化，这些变化会极大地影响RPP。筛选阶段包括实验设计（DoE）方法，评估其理论基础，实施和局限性。对于建模，我们描述了整合实验数据以开发智能介质配方预测模型的方法。然后可以使用基于模型的优化策略为给定的应用程序选择最佳的媒体组合。验证阶段的目的是评估模型预测，并为模型训练和改进提供反馈。最后，我们调查了机械和人工智能/机器学习（AI/ML）驱动的模型，作为生物过程条件、营养可用性、细胞代谢和蛋白质质量预测建模的集成转换工具，目的是优化培养基，提高蛋白质产量，同时降低成本和环境影响。最后，我们解决了将实验室规模的介质优化转化为工业规模设置的挑战，并探索了未来人工智能/机器学习驱动的方法，这些方法可能克服当前的瓶颈，并加速RPP的介质设计。总之，这篇综述为推进RPP中的智能介质设计提供了一个统一的框架。

{"title":"Smart culture medium optimization for recombinant protein production: Experimental, modeling, and AI/ML-driven strategies","authors":"Galib Khan , Carrie Sanford , Cong T. Trinh","doi":"10.1016/j.biotechadv.2025.108738","DOIUrl":"10.1016/j.biotechadv.2025.108738","url":null,"abstract":"<div><div>Recombinant protein production (RPP) is central to biotechnology, where recombinant proteins are used as either end products or catalysts in the synthesis of chemicals, fuels, and materials. Among the major cost drivers, culture medium plays a pivotal role in determining protein yield and quality. This review presents a comprehensive perspective on the critical stages of “smart” culture medium optimization: planning, screening, modeling, optimization, and validation. In the planning stage, we examine the nutritional and energetic roles of medium components, including carbon, nitrogen, amino acids, salts, and trace metals, and their impacts on culture parameters such as pH, oxidative state, and osmolality. We highlight the variability in trace metal content due to water sources, culture vessels, and raw materials, which can substantially influence RPP. The screening stage covers Design of Experiments (DoE) approaches, assessing their theoretical basis, implementation, and limitations. For modeling, we describe methods that integrate experimental data to develop predictive models for smart medium formulation. Model-based optimization strategies can then be employed to select optimal media compositions for a given application. The validation stage aims to evaluate model predictions and provide feedback for model training and refinement. Finally, we survey mechanistic and artificial intelligence/machine learning (AI/ML)-driven models as integrated, transformational tools for predictive modeling of bioprocess conditions, nutrient availability, cellular metabolism, and protein quality, with the goal of optimizing culture media to enhance protein yields while reducing costs and environmental impact. We conclude by addressing the challenges of translating laboratory-scale medium optimization to industrial-scale settings and exploring future AI/ML-driven approaches that may overcome current bottlenecks and accelerate medium design for RPP. Overall, this review provides a unified framework for advancing smart medium design in RPP.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"86 ","pages":"Article 108738"},"PeriodicalIF":12.5,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145312343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circular RNAs in microalgae: Uncovering their biological significance 微藻中的环状rna：揭示其生物学意义。

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-10-15 DOI: 10.1016/j.biotechadv.2025.108739

Jaber Dehghani , Bahman Panahi , Jens Mortansson Jelstrup Nolsøe , Patrice Lerouge , Muriel Bardor , Yadollah Omidi

Circular RNAs (circRNAs) have recently garnered significant attention due to their emerging regulatory roles across eukaryotic organisms. These non-coding RNA molecules are generated through a non-canonical back-splicing mechanism that covalently joins the 5′ and 3′ ends, resulting in a closed-loop structure. Although the complete functional landscape of circRNAs remains to be elucidated, advances in RNA sequencing technologies and computational biology have accelerated their identification and functional annotation in both plant and mammalian systems. CircRNAs are increasingly implicated in the regulation of key cellular and metabolic processes, including transcription, translation, protein-protein interactions, cellular proliferation, development, and stress responses, thereby contributing to homeostasis and survival. In this study, we present a comprehensive overview of circRNA biogenesis, structural features, biological roles, and bioinformatic tools used for their prediction in eukaryotes. However, no prior studies have systematically characterized circRNAs in microalgae. To address this gap, we analyzed RNA-seq datasets from three phylogenetically distinct microalgal species (e.g., Chlamydomonas reinhardtii, Dunaliella salina, and Phaeodactylum tricornutum) using the CIRI2 pipeline to identify putative circRNAs. Our analysis revealed candidate circRNAs potentially involved in essential biological pathways, including RNA transcription regulation, mRNA splicing, translational control, chlorophyll function, cytochrome c maintenance, and various post-transcriptional and post-translational modifications. These findings offer novel insights into the regulatory landscape of circRNAs in microalgal species and lay the groundwork for future functional investigations.

环状rna （circRNAs）最近因其在真核生物中的新兴调控作用而引起了极大的关注。这些非编码RNA分子是通过非规范的反向剪接机制产生的，该机制将5‘和3’端共价连接，形成闭环结构。尽管环状RNA的完整功能景观仍有待阐明，但RNA测序技术和计算生物学的进步已经加速了它们在植物和哺乳动物系统中的鉴定和功能注释。CircRNAs越来越多地参与关键细胞和代谢过程的调控，包括转录、翻译、蛋白-蛋白相互作用、细胞增殖、发育和应激反应，从而有助于体内平衡和生存。在这项研究中，我们全面概述了真核生物中circRNA的生物发生、结构特征、生物学作用以及用于预测circRNA的生物信息学工具。然而，之前没有研究系统地表征微藻中的环状rna。为了解决这一空白，我们使用CIRI2管道分析了来自三种不同系统发育的微藻物种（如莱因哈衣藻、盐藻Dunaliella salina和三角褐藻Phaeodactylum tricornutum）的RNA-seq数据集，以鉴定推测的环状rna。我们的分析揭示了候选环状RNA可能参与必要的生物学途径，包括RNA转录调控、mRNA剪接、翻译控制、叶绿素功能、细胞色素c维持以及各种转录后和翻译后修饰。这些发现为微藻物种中环状rna的调控格局提供了新的见解，并为未来的功能研究奠定了基础。

{"title":"Circular RNAs in microalgae: Uncovering their biological significance","authors":"Jaber Dehghani , Bahman Panahi , Jens Mortansson Jelstrup Nolsøe , Patrice Lerouge , Muriel Bardor , Yadollah Omidi","doi":"10.1016/j.biotechadv.2025.108739","DOIUrl":"10.1016/j.biotechadv.2025.108739","url":null,"abstract":"<div><div>Circular RNAs (circRNAs) have recently garnered significant attention due to their emerging regulatory roles across eukaryotic organisms. These non-coding RNA molecules are generated through a non-canonical back-splicing mechanism that covalently joins the 5′ and 3′ ends, resulting in a closed-loop structure. Although the complete functional landscape of circRNAs remains to be elucidated, advances in RNA sequencing technologies and computational biology have accelerated their identification and functional annotation in both plant and mammalian systems. CircRNAs are increasingly implicated in the regulation of key cellular and metabolic processes, including transcription, translation, protein-protein interactions, cellular proliferation, development, and stress responses, thereby contributing to homeostasis and survival. In this study, we present a comprehensive overview of circRNA biogenesis, structural features, biological roles, and bioinformatic tools used for their prediction in eukaryotes. However, no prior studies have systematically characterized circRNAs in microalgae. To address this gap, we analyzed RNA-seq datasets from three phylogenetically distinct microalgal species (e.g., <em>Chlamydomonas reinhardtii</em>, <em>Dunaliella salina</em>, and <em>Phaeodactylum tricornutum</em>) using the CIRI2 pipeline to identify putative circRNAs. Our analysis revealed candidate circRNAs potentially involved in essential biological pathways, including RNA transcription regulation, mRNA splicing, translational control, chlorophyll function, cytochrome <em>c</em> maintenance, and various post-transcriptional and post-translational modifications. These findings offer novel insights into the regulatory landscape of circRNAs in microalgal species and lay the groundwork for future functional investigations.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"86 ","pages":"Article 108739"},"PeriodicalIF":12.5,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145312350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the enzymatic landscape of 4-α-glucanotransferases in carbohydrate bioprocessing 探索碳水化合物生物加工中4-α-葡萄糖基转移酶的酶景观。

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-10-14 DOI: 10.1016/j.biotechadv.2025.108737

Yu Wang , Yu Tian , Marie Sofie Møller , Zhengyu Jin , Xiaoxiao Li , Birte Svensson

4-α-Glucanotransferases (4αGTs), also known as amylomaltases or disproportionating enzymes, catalyze α-1,4-glucan and maltooligosaccharide transfer in starch and glycogen metabolism of microorganisms, plants and animals. The present review covers their classification, reactions, structure-function relationships, engineering and applications. 4αGTs belong to glycoside hydrolase families GH13, GH57, and GH77, and catalyze four types of reactions: disproportionation, cyclization, coupling, and hydrolysis, of which the first two are particularly important for biotechnological applications. Insights into active site structures and substrate binding have facilitated the rational design of product specificity, modes of action, and increased product yields. Assays of the four reactions encompass monitoring amylose consumption by iodine staining, release of glucose in maltotriose disproportionation, chromatographic analysis of change in chain lengths, and release of reducing sugar by hydrolysis. Major reactions in transglycosylation of starch include formation of amylopectin with extended branch chains and cyclization to large-ring cyclodextrins (LR-CDs), also referred to as cycloamyloses (CAs). Product yields, chain length distribution, and size of LR-CDs depend on the enzyme, substrates and reaction conditions. 4αGT products are useful in the food, biomaterials and pharma sectors. Thus, chain length modification can elicit resistance of starch to digestion via structural reorganization and confer thermo-reversible gel formation, while LR-CDs can increase aqueous solubility of guest-molecules for controlled delivery and adjust rheological behavior of starches. Moreover, 4αGT can generate bioactive glycoconjugates and novel oligosaccharides by transglycosylation. Future development of 4αGT-catalyzed reactions includes optimization by rational enzyme engineering and high-throughput screening technologies. This review portrays the immense potential of 4αGTs in sustainable biomanufacturing.

4-α-葡聚糖转移酶（4α gts），又称淀粉酶或歧化酶，在微生物、植物和动物的淀粉和糖原代谢中催化α-1,4-葡聚糖和麦芽糖低聚糖的转移。综述了它们的分类、反应、构效关系、工程及应用。4α gt属于糖苷水解酶家族GH13、GH57和GH77，可催化歧化、环化、偶联和水解四种反应，其中前两种反应在生物技术应用中尤为重要。对活性位点结构和底物结合的深入了解有助于合理设计产品特异性、作用模式和提高产品产量。这四种反应的测定包括用碘染色法监测直链淀粉的消耗、麦芽糖歧化过程中葡萄糖的释放、链长变化的色谱分析以及水解释放还原糖。淀粉转糖基化的主要反应包括支链延长的支链淀粉的形成和环化成大环环糊精（LR-CDs），也被称为环直链淀粉（CAs）。产物产率、链长分布和LR-CDs的大小取决于酶、底物和反应条件。4αGT产品在食品、生物材料和制药领域都很有用。因此，链长修饰可以通过结构重组引起淀粉对消化的抗性，并赋予热可逆凝胶形成，而LR-CDs可以增加客体分子的水溶性，以控制递送并调节淀粉的流变行为。此外，4αGT可以通过转糖基化生成具有生物活性的糖缀合物和新型低聚糖。4α gt催化反应的未来发展包括合理的酶工程优化和高通量筛选技术。这篇综述描绘了4α gt在可持续生物制造中的巨大潜力。

{"title":"Exploring the enzymatic landscape of 4-α-glucanotransferases in carbohydrate bioprocessing","authors":"Yu Wang , Yu Tian , Marie Sofie Møller , Zhengyu Jin , Xiaoxiao Li , Birte Svensson","doi":"10.1016/j.biotechadv.2025.108737","DOIUrl":"10.1016/j.biotechadv.2025.108737","url":null,"abstract":"<div><div>4-α-Glucanotransferases (4αGTs), also known as amylomaltases or disproportionating enzymes, catalyze α-1,4-glucan and maltooligosaccharide transfer in starch and glycogen metabolism of microorganisms, plants and animals. The present review covers their classification, reactions, structure-function relationships, engineering and applications. 4αGTs belong to glycoside hydrolase families GH13, GH57, and GH77, and catalyze four types of reactions: disproportionation, cyclization, coupling, and hydrolysis, of which the first two are particularly important for biotechnological applications. Insights into active site structures and substrate binding have facilitated the rational design of product specificity, modes of action, and increased product yields. Assays of the four reactions encompass monitoring amylose consumption by iodine staining, release of glucose in maltotriose disproportionation, chromatographic analysis of change in chain lengths, and release of reducing sugar by hydrolysis. Major reactions in transglycosylation of starch include formation of amylopectin with extended branch chains and cyclization to large-ring cyclodextrins (LR-CDs), also referred to as cycloamyloses (CAs). Product yields, chain length distribution, and size of LR-CDs depend on the enzyme, substrates and reaction conditions. 4αGT products are useful in the food, biomaterials and pharma sectors. Thus, chain length modification can elicit resistance of starch to digestion via structural reorganization and confer thermo-reversible gel formation, while LR-CDs can increase aqueous solubility of guest-molecules for controlled delivery and adjust rheological behavior of starches. Moreover, 4αGT can generate bioactive glycoconjugates and novel oligosaccharides by transglycosylation. Future development of 4αGT-catalyzed reactions includes optimization by rational enzyme engineering and high-throughput screening technologies. This review portrays the immense potential of 4αGTs in sustainable biomanufacturing.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"86 ","pages":"Article 108737"},"PeriodicalIF":12.5,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding bioprocesses with transcriptomics: current status and future potential 用转录组学解码生物过程：现状和未来潜力。

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-10-13 DOI: 10.1016/j.biotechadv.2025.108736

Nadja Alina Henke , Boas Pucker , Alexander Grünberger

Transcriptomic analyses represent widely used state-of-the art methodologies in molecular biosciences going back to the early 1960s. Over the last years, transcriptomics has become increasingly important in the field of bioprocess engineering. Systematic transcriptomics platform technologies (especially RNA-seq) play an accelerating role to investigate the gene expression profiles of cells in diverse bioprocesses, covering prokaryotic and eukaryotic cell systems. This review summarizes how different transcriptomics methodologies from RT-qPCR to microarrays and RNA-seq have been applied in bioprocess engineering to date. The major scopes of the reviewed works can be categorized as the following: strain/cell line characterization, investigation of culture/media conditions, process operations as well as scale-up/down studies. Subsequently, a perspective is given how emerging sequencing-based transcriptomics could envision the understanding of population diversities in space and time aided by single cell analysis (scRNA-seq) as well as transcriptional histories (Record-Seq).

转录组学分析代表了分子生物科学中广泛使用的最新方法，可以追溯到20世纪60年代初。在过去的几年里，转录组学在生物过程工程领域变得越来越重要。系统转录组学平台技术（特别是RNA-seq）在研究多种生物过程中细胞的基因表达谱方面发挥着加速作用，涵盖了原核和真核细胞系统。本文综述了迄今为止从RT-qPCR到微阵列和RNA-seq等不同的转录组学方法在生物工艺工程中的应用。所审查的工作的主要范围可分为以下几类：菌株/细胞系特性、培养/培养基条件调查、工艺操作以及放大/缩小研究。随后，给出了新兴的基于测序的转录组学如何在单细胞分析（scRNA-seq）和转录历史（Record-Seq）的帮助下，设想对空间和时间上种群多样性的理解。

引用次数: 0

Decoding microbial interactions: Interaction networks and regulatory strategies for medium-chain fatty acid biosynthesis through anaerobic chain elongation 解码微生物相互作用：通过厌氧链延伸的中链脂肪酸生物合成的相互作用网络和调节策略。

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-10-12 DOI: 10.1016/j.biotechadv.2025.108735

Yi Liang, Jiadong Yu , Zonglu Yao, Yuxuan Sun, Jing Feng, Ruixia Shen, Juan Luo, Lixin Zhao

The anaerobic biosynthesis of medium-chain fatty acids (MCFAs) as valorized bio-based chemicals relies on intricate and dynamic interaction networks within microbial communities. This review systematically summarizes the key mechanisms and regulatory strategies driving MCFA biosynthesis in terms of microbial interactions, with a focus on electron donor-acceptor generation and chain elongation (CE) processes. The functional stability and resilience of anaerobic fermentation systems are collectively sustained by microbial diversity via modular functional partitioning, metabolic complementarity, resilience against perturbations, and environmental adaptation. Notably, substrate competition and syntrophic symbiosis between functional taxa directly govern the directionality and efficiency of the metabolic flux. Carbon source preferences and environmental factors synergistically steer pathway selection, while exogenous interventions such as enhanced electron transfer or niche occupation optimize microbial cooperation. In addition, quorum sensing and electrochemical synergy further balance inter-species competition to achieve a dynamic equilibrium between metabolic branch inhibition and enrichment of CE consortia. These multidimensional interaction mechanisms provide high-purity electron donors and stable metabolic foundations for MCFA synthesis to guide directional microbial engineering strategies to enhance product yields. This study systematically summarized how microbial interaction networks drive efficient MCFA biosynthesis via a multi-scale coordination between various mechanisms, including metabolic flux partitioning control, environmental response feedback, and functional modularization design, providing a theoretical foundation for resolving critical challenges during anaerobic MCFA fermentation.

中链脂肪酸（MCFAs）作为生物基化学物的厌氧生物合成依赖于微生物群落内复杂和动态的相互作用网络。本文从微生物相互作用的角度系统地总结了MCFA生物合成的关键机制和调控策略，重点介绍了电子供体-受体生成和链延伸（CE）过程。厌氧发酵系统的功能稳定性和弹性是由微生物多样性通过模块化功能分配、代谢互补、对扰动的弹性和环境适应来共同维持的。值得注意的是，功能类群之间的底物竞争和共生关系直接决定了代谢通量的方向性和效率。碳源偏好和环境因素协同引导途径选择，而外源干预如增强电子转移或生态位占据优化微生物合作。此外，群体感应和电化学协同进一步平衡了种间竞争，实现了代谢分支抑制与CE联合体富集之间的动态平衡。这些多维相互作用机制为MCFA合成提供了高纯度的电子供体和稳定的代谢基础，指导定向微生物工程策略以提高产品收率。本研究系统总结了微生物相互作用网络如何通过代谢通量分配控制、环境响应反馈和功能模块化设计等多种机制之间的多尺度协调，推动高效的MCFA生物合成，为解决厌氧MCFA发酵过程中的关键挑战提供理论基础。

{"title":"Decoding microbial interactions: Interaction networks and regulatory strategies for medium-chain fatty acid biosynthesis through anaerobic chain elongation","authors":"Yi Liang, Jiadong Yu , Zonglu Yao, Yuxuan Sun, Jing Feng, Ruixia Shen, Juan Luo, Lixin Zhao","doi":"10.1016/j.biotechadv.2025.108735","DOIUrl":"10.1016/j.biotechadv.2025.108735","url":null,"abstract":"<div><div>The anaerobic biosynthesis of medium-chain fatty acids (MCFAs) as valorized bio-based chemicals relies on intricate and dynamic interaction networks within microbial communities. This review systematically summarizes the key mechanisms and regulatory strategies driving MCFA biosynthesis in terms of microbial interactions, with a focus on electron donor-acceptor generation and chain elongation (CE) processes. The functional stability and resilience of anaerobic fermentation systems are collectively sustained by microbial diversity via modular functional partitioning, metabolic complementarity, resilience against perturbations, and environmental adaptation. Notably, substrate competition and syntrophic symbiosis between functional taxa directly govern the directionality and efficiency of the metabolic flux. Carbon source preferences and environmental factors synergistically steer pathway selection, while exogenous interventions such as enhanced electron transfer or niche occupation optimize microbial cooperation. In addition, quorum sensing and electrochemical synergy further balance inter-species competition to achieve a dynamic equilibrium between metabolic branch inhibition and enrichment of CE consortia. These multidimensional interaction mechanisms provide high-purity electron donors and stable metabolic foundations for MCFA synthesis to guide directional microbial engineering strategies to enhance product yields. This study systematically summarized how microbial interaction networks drive efficient MCFA biosynthesis via a multi-scale coordination between various mechanisms, including metabolic flux partitioning control, environmental response feedback, and functional modularization design, providing a theoretical foundation for resolving critical challenges during anaerobic MCFA fermentation.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"86 ","pages":"Article 108735"},"PeriodicalIF":12.5,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding biocatalytic reactivity landscapes by physical fields and chemical strategies for green manufacturing 通过物理领域和化学策略扩大绿色制造的生物催化反应性景观。

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-10-11 DOI: 10.1016/j.biotechadv.2025.108734

Renjie Li, Hanbing Feng, Miao Shi, Aodi Zhang, Su Jing

The burgeoning demand for green manufacturing has spurred significant interest in harnessing biocatalysis across diverse sectors for the synthesis of high value-added compounds. Despite the inherent utility of biocatalysts, their application is often circumscribed by the specificity of the natural enzymes involved. To transcend these limitations, innovative physical and chemical methodologies have been integrated into biocatalytic processes. Physical field interventions, such as the application of mechanical force (force), temperature (heat), light, electricity, and ultrasound (sound) have been employed to engineer reaction systems that facilitate the transformation of substrates into target compounds. On the chemical frontier, metal catalysis, small molecule organocatalysis coupled with biocatalysis in cascade reactions have been revolutionized through several strategies: (I) the one-pot approach, where products are isolated in a stepwise manner by the metal catalysis and biocatalysis; (II) the sequential cascade reaction within a single vessel, wherein enzymes and metal catalysts are introduced simultaneously to amplify product yield; and (III) the cooperative reaction paradigm, where the metal catalyst's product serves as an intermediate or substrate that synergistically interacts with the enzyme to yield the desired product in a one-pot synthesis. This review outlines the research progress in physical and chemical techniques in biocatalysis, aiming to further broaden the reactivity profile of natural enzymes and thereby produce more desired chemicals in past years.

对绿色制造的快速增长的需求激发了人们对利用不同部门的生物催化来合成高附加值化合物的极大兴趣。尽管生物催化剂具有固有的效用，但它们的应用往往受到所涉及的天然酶的特异性的限制。为了超越这些限制，创新的物理和化学方法已被整合到生物催化过程中。物理场干预，如机械力（力）、温度（热）、光、电和超声波（声）的应用，已被用于设计促进底物转化为目标化合物的反应系统。在化学前沿，金属催化、小分子有机催化和级联反应中的生物催化已经通过以下几种策略发生了革命性的变化：(1)一锅法，即通过金属催化和生物催化逐步分离产物；（II）单个容器内的顺序级联反应，其中同时引入酶和金属催化剂以提高产品收率；（III）协同反应模式，其中金属催化剂的产物作为中间体或底物，与酶协同相互作用，在一锅合成中产生所需的产物。本文综述了近年来生物催化物理和化学技术的研究进展，旨在进一步拓宽天然酶的反应谱，从而生产出更多需要的化学物质。

{"title":"Expanding biocatalytic reactivity landscapes by physical fields and chemical strategies for green manufacturing","authors":"Renjie Li, Hanbing Feng, Miao Shi, Aodi Zhang, Su Jing","doi":"10.1016/j.biotechadv.2025.108734","DOIUrl":"10.1016/j.biotechadv.2025.108734","url":null,"abstract":"<div><div>The burgeoning demand for green manufacturing has spurred significant interest in harnessing biocatalysis across diverse sectors for the synthesis of high value-added compounds. Despite the inherent utility of biocatalysts, their application is often circumscribed by the specificity of the natural enzymes involved. To transcend these limitations, innovative physical and chemical methodologies have been integrated into biocatalytic processes. Physical field interventions, such as the application of mechanical force (force), temperature (heat), light, electricity, and ultrasound (sound) have been employed to engineer reaction systems that facilitate the transformation of substrates into target compounds. On the chemical frontier, metal catalysis, small molecule organocatalysis coupled with biocatalysis in cascade reactions have been revolutionized through several strategies: (I) the one-pot approach, where products are isolated in a stepwise manner by the metal catalysis and biocatalysis; (II) the sequential cascade reaction within a single vessel, wherein enzymes and metal catalysts are introduced simultaneously to amplify product yield; and (III) the cooperative reaction paradigm, where the metal catalyst's product serves as an intermediate or substrate that synergistically interacts with the enzyme to yield the desired product in a one-pot synthesis. This review outlines the research progress in physical and chemical techniques in biocatalysis, aiming to further broaden the reactivity profile of natural enzymes and thereby produce more desired chemicals in past years.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"86 ","pages":"Article 108734"},"PeriodicalIF":12.5,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial quorum sensing: Mechanisms, applications, and challenges 微生物群体感应：机制、应用和挑战。

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-10-06 DOI: 10.1016/j.biotechadv.2025.108733

Qi Ruan , Shuting Geng , Jianqiu Yu , Leilei Lu , Yanhua Liu , Jianqiu Chen , Qianjiahua Liao , Ruixin Guo

Quorum sensing (QS) is a sophisticated microbial communication system that orchestrates gene expression in response to population density, governing collective behaviors crucial for microbial survival and function. This comprehensive review elucidates the intricate synthesis pathways and mechanisms of QS signaling molecules across diverse microbial species. We critically analyze the multifaceted applications of QS in healthcare, agriculture, and environmental biotechnology, highlighting its potential to revolutionize these fields. The review also explores quorum quenching (QQ) strategies as a novel approach to microbial control and examines the unique adaptations of QS systems in extreme environments. By synthesizing recent advancements and identifying knowledge gaps, we outline pressing challenges and propose promising future research directions. This work aims to provide a roadmap for leveraging QS in developing innovative biotechnological solutions to address global challenges in health, food security, and environmental sustainability.

群体感应（Quorum sensing， QS）是一种复杂的微生物通讯系统，它根据种群密度协调基因表达，控制对微生物生存和功能至关重要的集体行为。本文综述了QS信号分子在不同微生物物种中复杂的合成途径和机制。我们批判性地分析了QS在医疗保健、农业和环境生物技术方面的多方面应用，强调了它在这些领域的革命性潜力。这篇综述还探讨了群体猝灭（QQ）策略作为一种新的微生物控制方法，并研究了QS系统在极端环境中的独特适应性。通过综合最近的进展和识别知识差距，我们概述了紧迫的挑战，并提出了有希望的未来研究方向。这项工作旨在为利用QS开发创新生物技术解决方案提供路线图，以应对健康、粮食安全和环境可持续性方面的全球挑战。

{"title":"Microbial quorum sensing: Mechanisms, applications, and challenges","authors":"Qi Ruan , Shuting Geng , Jianqiu Yu , Leilei Lu , Yanhua Liu , Jianqiu Chen , Qianjiahua Liao , Ruixin Guo","doi":"10.1016/j.biotechadv.2025.108733","DOIUrl":"10.1016/j.biotechadv.2025.108733","url":null,"abstract":"<div><div>Quorum sensing (QS) is a sophisticated microbial communication system that orchestrates gene expression in response to population density, governing collective behaviors crucial for microbial survival and function. This comprehensive review elucidates the intricate synthesis pathways and mechanisms of QS signaling molecules across diverse microbial species. We critically analyze the multifaceted applications of QS in healthcare, agriculture, and environmental biotechnology, highlighting its potential to revolutionize these fields. The review also explores quorum quenching (QQ) strategies as a novel approach to microbial control and examines the unique adaptations of QS systems in extreme environments. By synthesizing recent advancements and identifying knowledge gaps, we outline pressing challenges and propose promising future research directions. This work aims to provide a roadmap for leveraging QS in developing innovative biotechnological solutions to address global challenges in health, food security, and environmental sustainability.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"86 ","pages":"Article 108733"},"PeriodicalIF":12.5,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environments contaminated by pesticides are hotspots for the selection of bacterial chassis for biotechnological applications 农药污染环境是生物技术应用中细菌底盘选择的热点。

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-10-03 DOI: 10.1016/j.biotechadv.2025.108723

Caroline Rosa Silva , Marcos Pileggi

Water storage tanks contaminated with pesticides serve as a model for an artificial ecosystem in which non-target species, particularly microorganisms, must develop various response mechanisms to survive in such environments. These mechanisms can be classified into non-specific responses, which are associated with various stressors, as well as specific responses to herbicides. Due to the stressful conditions present in these environments, they are regarded as hotspots for the selection of bacterial chassis or consortia of strains that possess combinations of genes encoding diverse phenotypes adapted for survival against a range of toxic substances. This literature review aims to extend the concept of hotspots to other aquatic and terrestrial environments contaminated with pesticides, while also discussing hypotheses regarding the potential exploitation of adapted phenotypes in biotechnological applications. These applications include bioprospecting for microorganisms that produce antimicrobial or antitumor agents, developing live biotherapeutic products for various diseases, and implementing bioremediation strategies. While well established, advances in omics technologies offer new opportunities to enhance the efficiency and safety of these strategies by manipulating gene regulatory systems. However, substantial investment is needed for genetic and metabolic manipulation. Thus, identifying selective hotspots is a beneficial strategy for obtaining viable chassis, as many organisms have already been selected in their ecosystems, along with detailing regulatory systems through omics approaches.

被农药污染的水箱可以作为人工生态系统的模型，在这种生态系统中，非目标物种，特别是微生物，必须发展出各种反应机制才能在这种环境中生存。这些机制可分为与各种应激源相关的非特异性反应和对除草剂的特异性反应。由于这些环境中存在的压力条件，它们被认为是选择细菌底盘或菌株联合体的热点，这些菌株具有编码不同表型的基因组合，适合于对抗一系列有毒物质的生存。本文献综述旨在将热点的概念扩展到其他受农药污染的水生和陆地环境，同时也讨论了关于适应表型在生物技术应用中潜在开发的假设。这些应用包括对生产抗微生物或抗肿瘤药物的微生物进行生物勘探，开发各种疾病的活生物治疗产品，以及实施生物修复策略。虽然已经建立，组学技术的进步为通过操纵基因调控系统来提高这些策略的效率和安全性提供了新的机会。然而，基因和代谢操作需要大量的投资。因此，确定选择性热点是获得可行底盘的有益策略，因为许多生物体已经在其生态系统中被选中，同时通过组学方法详细管理系统。

{"title":"Environments contaminated by pesticides are hotspots for the selection of bacterial chassis for biotechnological applications","authors":"Caroline Rosa Silva , Marcos Pileggi","doi":"10.1016/j.biotechadv.2025.108723","DOIUrl":"10.1016/j.biotechadv.2025.108723","url":null,"abstract":"<div><div>Water storage tanks contaminated with pesticides serve as a model for an artificial ecosystem in which non-target species, particularly microorganisms, must develop various response mechanisms to survive in such environments. These mechanisms can be classified into non-specific responses, which are associated with various stressors, as well as specific responses to herbicides. Due to the stressful conditions present in these environments, they are regarded as hotspots for the selection of bacterial chassis or consortia of strains that possess combinations of genes encoding diverse phenotypes adapted for survival against a range of toxic substances. This literature review aims to extend the concept of hotspots to other aquatic and terrestrial environments contaminated with pesticides, while also discussing hypotheses regarding the potential exploitation of adapted phenotypes in biotechnological applications. These applications include bioprospecting for microorganisms that produce antimicrobial or antitumor agents, developing live biotherapeutic products for various diseases, and implementing bioremediation strategies. While well established, advances in omics technologies offer new opportunities to enhance the efficiency and safety of these strategies by manipulating gene regulatory systems. However, substantial investment is needed for genetic and metabolic manipulation. Thus, identifying selective hotspots is a beneficial strategy for obtaining viable chassis, as many organisms have already been selected in their ecosystems, along with detailing regulatory systems through omics approaches.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"86 ","pages":"Article 108723"},"PeriodicalIF":12.5,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0