Biotechnology advances最新文献_第7页

Geminivirus vectors: From gene silencing to synthetic biology 双病毒载体：从基因沉默到合成生物学

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-11-29 DOI: 10.1016/j.biotechadv.2025.108771

Yi Zhang , Shulin Deng

Geminiviruses, the largest plant DNA virus family, cause devastating diseases in crops worldwide. These viruses possess distinctive features, such as the stem-loop structure and replication protein (Rep), which enable the creation of functional geminiviral replicons (GVRs) in plants. Over three decades, geminiviruses have been developed into vectors for virus-induced gene silencing (VIGS), high-level protein expression, and genome editing. This review introduces the genomic structure, Rep protein domains and functions, as well as the historical applications of geminiviruses, then highlights their prominent roles in VIGS and synthetic biology. As VIGS vectors, bipartite geminiviruses utilize AV1 gene replacement, while monopartite species rely on satellite DNAs to insert target sequences, enabling gene silencing in diverse plants. In synthetic biology, GVRs facilitate high-level protein expression through autonomous replication and enhance CRISPR/Cas genome editing efficiency in crops. Additionally, gene regulatory elements, including tissue-specific promoters and gene expression enhancement sequences from geminiviral genomes or satellite DNA expand their utility in genetic engineering. Finally, this review provides an outlook on the future development of geminivirus vectors. GVRs can work as plasmid-like DNAs for supporting diverse and creative designs in plant synthetic biology. The stem-loop structure and Rep are not unique to geminiviruses, a fact that suggests potential cross-kingdom applications of GVRs beyond plants. Vast viral resources enable further acceleration of GVR applications through resource mining and optimization. Moreover, attenuated or engineered geminiviral strains hold promise as “plant vaccines” via cross-protection. Collectively, geminivirus vectors bridge fundamental viral research with practical innovations in crop improvement, biomanufacturing, and synthetic biology.

双病毒是最大的植物DNA病毒家族，在世界各地的作物中造成毁灭性的疾病。这些病毒具有独特的特征，如茎环结构和复制蛋白（Rep），这使得能够在植物中产生功能性双病毒复制子（GVRs）。三十多年来，双病毒已发展成为病毒诱导基因沉默（VIGS）、高水平蛋白表达和基因组编辑的载体。本文介绍了双病毒的基因组结构、Rep蛋白结构域和功能，以及它们在VIGS和合成生物学中的重要作用。作为VIGS载体，二分双病毒利用AV1基因替代，而单分双病毒依靠卫星dna插入靶序列，在多种植物中实现基因沉默。在合成生物学中，GVRs通过自主复制促进高水平蛋白表达，提高作物CRISPR/Cas基因组编辑效率。此外，基因调控元件，包括来自双病毒基因组或卫星DNA的组织特异性启动子和基因表达增强序列，扩大了它们在基因工程中的应用。最后，对双病毒载体的研究进展进行了展望。gvr可以作为质粒一样的dna，支持植物合成生物学中多样化和创造性的设计。茎环结构和Rep并不是双病毒所独有的，这一事实表明GVRs可能在植物之外的跨界应用。通过资源挖掘和优化，大量的病毒资源可以进一步加速GVR应用。此外，减毒或工程化的双病毒毒株有望通过交叉保护成为“植物疫苗”。总的来说，双病毒载体将基础病毒研究与作物改良、生物制造和合成生物学方面的实际创新联系起来。

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引用次数: 0

Bridging photosynthesis and photovoltaics: Biotechnological pathways for sustainable solar energy 桥接光合作用和光伏：可持续太阳能的生物技术途径

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-11-28 DOI: 10.1016/j.biotechadv.2025.108768

Zhaodong Li , Zihui Gao , Haonan Song , Jialiang He , Wei Xiong

Integrating biological systems with artificial optoelectronic materials for efficient solar energy conversion has emerged as a cutting-edge and promising research direction in the pursuit of sustainable energy solutions. Natural photosynthesis, through intricate biological mechanisms, converts solar energy into chemical energy, serving as an inspiration for human innovation; concurrently, photovoltaic technologies utilize semiconductor materials to directly transform solar radiation into electricity. Recent interdisciplinary research efforts have led to the development of bio-abiotic hybrid interfaces, combining the regenerative capabilities of biological systems with the tunable optoelectronic properties of artificial materials, aiming to enhance solar energy conversion efficiency. This review focuses on the latest advancements in artificial photosynthesis, bio-photoelectrochemical systems, and bio-photovoltaic systems, emphasizing their potential to improve solar energy conversion efficiency. We explore the design principles, operational mechanisms, and performance metrics of these hybrid devices, and conduct an in-depth analysis of technical challenges such as interface stability and electron transfer efficiency. Furthermore, we propose future research directions to optimize these systems for practical applications in sustainable energy production. By integrating knowledge from biology, materials science, and energy engineering, we aim to provide new perspectives and strategies for the development of solar energy conversion technologies, advancing toward more efficient and sustainable energy solutions.

将生物系统与人工光电材料相结合，实现高效的太阳能转换，已成为追求可持续能源解决方案的前沿和有前途的研究方向。自然光合作用通过复杂的生物机制，将太阳能转化为化学能，为人类创新提供灵感；同时，光伏技术利用半导体材料将太阳辐射直接转化为电能。近年来，跨学科的研究努力导致了生物-非生物混合界面的发展，将生物系统的再生能力与人造材料的可调谐光电特性相结合，旨在提高太阳能转换效率。本文综述了人工光合作用、生物光电化学系统和生物光伏系统的最新进展，强调了它们在提高太阳能转换效率方面的潜力。我们探讨了这些混合器件的设计原理、运行机制和性能指标，并对界面稳定性和电子传递效率等技术挑战进行了深入分析。此外，我们提出了未来的研究方向，以优化这些系统在可持续能源生产中的实际应用。通过整合生物学，材料科学和能源工程的知识，我们的目标是为太阳能转换技术的发展提供新的视角和策略，朝着更高效和可持续的能源解决方案前进。

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引用次数: 0

Microbial computing: Review and Perspectives 微生物计算：回顾与展望

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-11-27 DOI: 10.1016/j.biotechadv.2025.108766

Paul Ahavi , Audrey Le Gouellec , Jean-Loup Faulon

Engineering microbial computers has been a longstanding endeavor in synthetic biology. Like other unconventional computing disciplines, the goal is to bring computation into real-world scenarios. Several potential applications in bioproduction, bioremediation, and biomedicine highlight the promise of this discipline. The first biocomputers were bottom-up predictable circuits that relied on a monoculture-based digital logic and were able to emulate simple logic gates. Drawing from computer theory and extending the analogy with conventional hardware has enabled the engineering of more complex circuits. However, this abstraction soon reached its limits and introduced a semantic gap, which, alongside the constraints imposed by the monoculture paradigm, led to significant scalability limitations such as metabolic burden, orthogonality issues and noisy expression. This review outlines the strategies developed to overcome these issues and engineer more complex biodevices: (i) mitigation strategies that focus on the optimization of the circuits, (ii) multicellular computing that distributes the metabolic load across a consortium and (iii) the implementation of more energy-efficient computing frameworks, such as analog and neuromorphic architectures. While these bottom-up strategies have yielded significant progress, they remain insufficient to emulate the computational complexity of the cellular signal-processing system. In this review, we additionally introduce a new perspective on biocomputing with a top-down approach named reservoir computing. This framework leverages the inherent dynamical computational capabilities and functionalities of biosystems to solve more complex and diverse tasks, thus offering a promising new path for engineering the next generation of microbial computers.

在合成生物学中，工程微生物计算机一直是一项长期的努力。与其他非常规计算学科一样，其目标是将计算带入现实世界。在生物生产、生物修复和生物医学方面的几个潜在应用突出了这一学科的前景。第一批生物计算机是自下而上的可预测电路，它依赖于基于单一文化的数字逻辑，能够模拟简单的逻辑门。从计算机理论出发，将其与传统硬件进行类比，使得设计更复杂的电路成为可能。然而，这种抽象很快就达到了极限，并引入了语义差距，这与单一文化范式所施加的约束一起，导致了显著的可扩展性限制，如代谢负担、正交性问题和嘈杂的表达。本综述概述了为克服这些问题和设计更复杂的生物设备而开发的策略：(i)侧重于优化电路的缓解策略，（ii）在整个联合体中分配代谢负荷的多细胞计算，以及（iii）实施更节能的计算框架，如模拟和神经形态架构。虽然这些自下而上的策略已经取得了重大进展，但它们仍然不足以模拟蜂窝信号处理系统的计算复杂性。在这篇综述中，我们还介绍了一种新的生物计算视角，即自上而下的水库计算方法。该框架利用生物系统固有的动态计算能力和功能来解决更复杂和多样化的任务，从而为工程下一代微生物计算机提供了一条有希望的新途径。

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引用次数: 0

Optogenetic tools for optimizing key signalling nodes in synthetic biology 优化合成生物学关键信号节点的光遗传学工具

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-11-27 DOI: 10.1016/j.biotechadv.2025.108770

Yuehui Tian , Shanshan Xu , Zidong Ye, Huiru Liu, Dongqing Wei, Hossain M. Zabed, Junhua Yun, Guoyan Zhang, Yufei Zhang, Cheng Zhang, Ruiqi Liu, Jia Li, Xianghui Qi

The modification of key enzymes for chemical production plays a crucial role in enhancing the yield of targeted products. However, manipulating key nodes in specific signalling pathways remains constrained by traditional gene overexpression or knockout strategies. Discovering and designing optogenetic tools enable us to regulate enzymatic activity or gene expression at key nodes in a spatiotemporal manner, rather than relying solely on chemical induction throughout production processes. In this review, we discuss the recent applications of optogenetic tools in the regulation of microbial metabolites, plant sciences and disease therapies. We categorize optogenetic tools into five classes based on their distinct applications. First, light-induced gene expression schedules can balance the trade-off between chemical production and cell growth phases. Second, light-triggered liquid-liquid phase separation (LLPS) modules provide opportunities to co-localize and condense key enzymes for enhancing catalytic efficiency. Third, light-induced subcellular localized photoreceptors enable the relocation of protein of interest across various subcellular compartments, allowing for the investigation of their dynamic regulatory processes. Fourth, light-regulated enzymes can dynamically regulate production of cyclic nucleotides or investigate endogenous components similar with conditional depletion or recovery function of protein of interest. Fifth, light-gated ion channels and pumps can be utilized to investigate dynamic ion signalling cascades in both animals and plants, or to boost ATP accumulation for enhancing biomass or bioproduct yields in microorganisms. Overall, this review aims to provide a comprehensive overview of optogenetic strategies that have the potential to advance both basic research and bioindustry within the field of synthetic biology.

化工生产中关键酶的修饰对提高目标产品的收率起着至关重要的作用。然而，操纵特定信号通路中的关键节点仍然受到传统基因过表达或敲除策略的限制。发现和设计光遗传学工具使我们能够以时空方式调节关键节点的酶活性或基因表达，而不是仅仅依赖于整个生产过程中的化学诱导。本文综述了近年来光遗传学工具在微生物代谢调控、植物科学和疾病治疗等方面的应用。我们将光遗传学工具根据其不同的应用分为五类。首先，光诱导的基因表达时间表可以平衡化学生产和细胞生长阶段之间的权衡。其次，光触发的液-液相分离（LLPS）模块提供了共定位和浓缩关键酶的机会，以提高催化效率。第三，光诱导的亚细胞定位光感受器使感兴趣的蛋白质能够在不同的亚细胞区室中重新定位，从而允许研究它们的动态调节过程。第四，光调节酶可以动态调节环核苷酸的产生或研究内源性成分，类似于感兴趣蛋白质的条件耗尽或恢复功能。第五，光门控离子通道和泵可用于研究动物和植物中的动态离子信号级联，或促进ATP积累以提高微生物的生物量或生物产品产量。总之，这篇综述的目的是提供一个全面的概述光遗传策略，有可能推动基础研究和生物产业在合成生物学领域。

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引用次数: 0

Progress in photo-enzyme coupling catalysis for carbon dioxide reduction 光-酶偶联催化二氧化碳还原研究进展

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-11-26 DOI: 10.1016/j.biotechadv.2025.108769

Ning Liu, Wenfang Liu

By mimicking natural photosynthesis, the photo-enzyme coupling catalysis (PECC) for carbon dioxide conversion integrates the advantages of photocatalysis and enzymatic catalysis, offering an effective and innovative pathway for capture and utilization of greenhouse gas. This review provides a comprehensive overview of recent advancements in this technology, covering the fundamental principles, key components, synergistic mechanisms, compatibility, and future perspectives. A photo-enzyme coupling system (PECS) can be categorized into cofactor-dependent or cofactor-independent system based on the requirement for cofactor mediation. Its main components include photocatalyst and enzyme, which demonstrates unique advantage in the synergism of energy transfer and substrate activation. In order to improve the compatibility of PECS, the strategies including compartmentalized immobilization and process optimization are employed. By developing highly efficient photocatalyst, strengthening interfacial interaction, and optimizing enzyme engineering, PECC holds great promise for transitioning from laboratory research to industrial application, providing robust support for mitigating global climate change and addressing energy crisis.

光-酶耦合催化（PECC）通过模拟自然光合作用，将光催化和酶催化的优点结合在一起，为温室气体的捕获和利用提供了一条有效的创新途径。本文综述了该技术的最新进展，包括基本原理、关键成分、协同机制、兼容性和未来前景。光-酶偶联系统根据对辅助因子的要求可分为辅助因子依赖性系统和辅助因子非依赖性系统。它的主要成分是光催化剂和酶，在能量传递和底物活化的协同作用方面具有独特的优势。为了提高PECS的相容性，采用了分区固定和工艺优化策略。通过开发高效光催化剂、加强界面相互作用、优化酶工程，PECC有望从实验室研究向工业应用过渡，为减缓全球气候变化和应对能源危机提供有力支持。

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引用次数: 0

From dye exclusion to high-throughput screening: A review of cell viability assays and their applications 从染料排除到高通量筛选：细胞活力测定及其应用综述

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-11-21 DOI: 10.1016/j.biotechadv.2025.108764

Kumar Vishven Naveen , Akanksha Tyagi , Omnia Mohammed Hamid Ibrahium, Rainer E.A.W. Fischer, Raluca Ostafe

Cell viability assays (CVAs) are widely used in cell biology, biomedical research, drug development, and biotechnology to assess cell health, proliferation, cytotoxicity, and functional activity under various conditions. Key applications span from everyday cell culture monitoring to drug screening and toxicology studies, immunology, vaccine development, and stem cell and regenerative medicine. Despite the long history and widespread use of CVAs, selecting the right assay remains a challenge for researchers. The increasing number of available assay options has led to confusion and inefficiencies, as scientists struggle to navigate the differences, trade-offs, and technical limitations of each method. Many researchers continue using the assays they were trained with, rather than exploring newer, more sensitive, or more appropriate techniques. Lab protocols are often passed down without reassessment, and new projects frequently adopt assays based on convenience (e.g., reagent availability or existing equipment) rather than rational selection criteria. Some groups deliberately choose less sensitive assays under the assumption that they produce “better-distributed” data. However, this incorrect justification arises because assays with a high limit of detection (LOD) fail to capture small variations, creating the misleading perception of clean and well-distributed data. Ignoring small variations does not improve accuracy - it simply reduces sensitivity, potentially leading to incorrect conclusions. Hence, the purpose of this review is to provide a comprehensive overview of contemporary CVAs by categorizing detection methods and summarizing their concepts, applications, benefits, and limitations, while also highlighting the potential need for novel approaches in this field. To assist researchers in selecting the most appropriate assay for their experimental goals, we also present a visual decision tree that integrates mechanistic insights with practical considerations.

细胞活力测定（cva）广泛应用于细胞生物学、生物医学研究、药物开发和生物技术，以评估各种条件下的细胞健康、增殖、细胞毒性和功能活性。主要应用范围从日常细胞培养监测到药物筛选和毒理学研究、免疫学、疫苗开发、干细胞和再生医学。尽管CVAs具有悠久的历史和广泛的使用，但选择正确的检测方法仍然是研究人员面临的一个挑战。越来越多的可用检测选项导致了混乱和低效率，因为科学家们很难驾驭每种方法的差异、权衡和技术限制。许多研究人员继续使用他们训练时使用的检测方法，而不是探索更新、更灵敏或更合适的技术。实验室方案通常在没有重新评估的情况下传递，新项目经常采用基于便利性（例如，试剂可用性或现有设备）而不是合理选择标准的分析方法。一些小组故意选择敏感度较低的分析方法，假设它们能产生“更好分布”的数据。然而，出现这种不正确的理由是因为具有高检测限（LOD）的分析不能捕获小的变化，从而产生了对干净和分布良好的数据的误导看法。忽略微小的变化并不能提高准确性——它只会降低灵敏度，可能导致错误的结论。因此，本综述的目的是通过对检测方法进行分类，总结其概念、应用、优点和局限性，对当代cva进行全面概述，同时也强调了该领域对新方法的潜在需求。为了帮助研究人员为他们的实验目标选择最合适的检测方法，我们还提出了一个可视化的决策树，该决策树将机械见解与实际考虑相结合。

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引用次数: 0

Biosynthesis of high-value chorismate derivatives in Escherichia coli: Recent advances and perspectives 高价值氯酸盐衍生物在大肠杆菌中的生物合成：最新进展和展望

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-11-20 DOI: 10.1016/j.biotechadv.2025.108762

Xuewei Pan , Shuran Jiang , Mi Tang , Na Wang , Qisheng Sun , Taowei Yang , Zhiming Rao

Chorismate serves not only as a critical branch point for aromatic amino acids biosynthesis, but also as an essential precursor for various high-value aromatic compounds. Currently, the sustainable production of chorismate derivatives is attracting escalating interest due to their pivotal roles as vital components in pharmaceuticals, cosmetics, and specialty chemical intermediates. However, these compounds are commonly obtained through plant extraction or chemical synthesis, which suffer from complex extraction processes, low yields, and environmental burdens, often leading to supply constraints. Therefore, to overcome these challenges, an increasing number of studies are focusing on exploring more production routes for chorismate derivatives to achieve green and efficient synthesis. With the advancement of systems metabolic engineering and contemporary synthetic biology, genetically redesigned Escherichia coli has become pivotal microbial platforms for chorismate derivative biosynthesis. This review outlines the significance of chorismate derivatives and their de novo biosynthetic pathways in E. coli, while reviewing the latest metabolic engineering and synthetic biology strategies employed to boost production of these compounds. Additionally, the persisting challenges and emerging opportunities in leveraging engineered E. coli platforms for industrial-scale biosynthesis of these high-value compounds are discussed.

choris酸盐不仅是芳香氨基酸生物合成的关键分支点，而且是各种高价值芳香化合物的重要前体。目前，由于在医药、化妆品和特殊化学中间体中扮演着重要的角色，氯酸盐衍生物的可持续生产正引起越来越多的关注。然而，这些化合物通常通过植物提取或化学合成获得，这些化合物受到提取过程复杂，产量低和环境负担的影响，往往导致供应限制。因此，为了克服这些挑战，越来越多的研究集中在探索更多的氯酸盐衍生物的生产路线，以实现绿色高效的合成。随着系统代谢工程和当代合成生物学的发展，经过基因改造的大肠杆菌已成为choris酸衍生物生物合成的关键微生物平台。本文概述了choris酸衍生物及其在大肠杆菌中的新生物合成途径的意义，同时综述了用于促进这些化合物生产的最新代谢工程和合成生物学策略。此外，还讨论了利用工程大肠杆菌平台进行工业规模生物合成这些高价值化合物的持续挑战和新机遇。

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引用次数: 0

Advances in transcription factor-based biosensors for natural product biosynthesis: Optimization, emerging technologies, and future prospects 基于转录因子的天然产物生物合成生物传感器研究进展：优化、新兴技术和未来展望

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-11-20 DOI: 10.1016/j.biotechadv.2025.108765

Xiaohan Zhang , Xinping Wu , Hui Chen , Qingsong Shao , Zheng Ma , Yefei Wu , Shengliang Wu , Lianghong Yin , Nana Ding

Natural products (NPs) are increasingly applied in food, medicine, and biotechnology. However, their biosynthesis remains constrained by low titers and yields. Transcription factor-based biosensors (TFBs) can convert biological signals into measurable outputs, enabling real-time monitoring and dynamic regulation of biosynthetic pathways, thereby facilitating the overcoming of these limitations. This review highlights recent advances in applying TFBs to NP production, with a focus on high-throughput screening, adaptive evolution, and dynamic control. We further discuss innovative engineering approaches aimed at optimizing TFB performance, including in silico TF identification, protein engineering, and fine-tuning of regulatory elements. Finally, we examine the challenges associated with using TFBs for microbial NP production and explore their potential in emerging platforms such as cell-free systems and non-model microorganisms. These insights offer valuable perspectives on overcoming the current limitations of biosensing technologies and advancing the scalable production of NPs.

天然产物在食品、医药、生物技术等领域的应用日益广泛。然而，它们的生物合成仍然受到低滴度和产量的限制。基于转录因子的生物传感器（TFBs）可以将生物信号转化为可测量的输出，实现生物合成途径的实时监测和动态调节，从而促进克服这些限制。本文综述了近年来TFBs在NP生产中的应用进展，重点介绍了高通量筛选、自适应进化和动态控制。我们进一步讨论了旨在优化TFB性能的创新工程方法，包括硅TF鉴定、蛋白质工程和调控元件的微调。最后，我们研究了与使用TFBs生产微生物NP相关的挑战，并探索了它们在新兴平台（如无细胞系统和非模式微生物）中的潜力。这些见解为克服当前生物传感技术的局限性和推进NPs的规模化生产提供了有价值的视角。

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引用次数: 0

Strategies to Enhance Stability of Cryopreservation Processes for Cell-Based Products 提高细胞基产品低温保存过程稳定性的策略

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-11-19 DOI: 10.1016/j.biotechadv.2025.108763

Yuki Uno , Yusuke Hayashi , Hirokazu Sugiyama , Jun Okuda , Tetsuji Nakamura , Masahiro Kino-oka

The projected expansion of the global market for cell manufacturing, which contributes to regenerative medicine and cell therapies, warrants the designing and development of scalable cryopreservation processes for cell-based products (CBPs) for use in both standard and personalized therapies. However, the change in scale causes variations in process parameters, which affects the stability of the CBP quality. Therefore, the cryopreservation process for CBPs needs to be designed based on the concept of cell manufacturability and consideration of both engineering and biological aspects. In this review, we discussed strategies to enhance the quality stability of CBPs during cryopreservation, focusing primarily on four key processes: dispensing, freezing, storage, and thawing. Additionally, we discussed the application of simulation technologies because they aid in constructing digital twins for the designing and development of the cryopreservation process and facilitate efficiency with limited time and resources.

预计全球细胞制造市场的扩张有助于再生医学和细胞治疗，这保证了设计和开发可扩展的细胞基产品（CBPs）冷冻保存工艺，用于标准和个性化治疗。然而，尺度的变化会引起工艺参数的变化，从而影响CBP质量的稳定性。因此，CBPs的低温保存工艺需要基于细胞可制造性的概念以及工程和生物学方面的考虑来设计。在这篇综述中，我们讨论了在冷冻保存过程中提高CBPs质量稳定性的策略，主要集中在四个关键过程：配药、冷冻、储存和解冻。此外，我们还讨论了模拟技术的应用，因为它们有助于构建数字双胞胎来设计和开发冷冻保存过程，并在有限的时间和资源下提高效率。

引用次数: 0

Harnessing spatial transcriptomics to understand host-parasite interactions in plants and animals 利用空间转录组学来了解植物和动物中宿主-寄生虫的相互作用

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-11-17 DOI: 10.1016/j.biotechadv.2025.108760

Anna Pijnacker , Christine W. Bruggeman , Hendrik C. Korswagen , Geert Smant , José L. Lozano-Torres

Obligate parasites pose a significant threat to animal, human, and plant health by affecting host gene expression through mechanisms that are poorly understood. Spatial transcriptomic technologies are revolutionizing our understanding of animal-parasite interactions, revealing tissue reorganization, cellular responses, and infection dynamics at a microscopic scale. These technologies also accelerate the identification of potential targets for treating animal parasite infections. Despite their potential, the application of spatial transcriptomic technologies to plant-parasite interactions is limited. This review highlights key challenges in applying spatial transcriptomics to plants. By drawing parallels with advances in animal systems, we discuss how spatial transcriptomics could contribute to localize and identify effectors, uncover the molecular mechanisms of plant-parasite infections, and find novel disease control targets. This cross-disciplinary perspective provides a roadmap for future research in plant and animal parasitology.

专性寄生虫通过影响宿主基因表达的机制对动物、人类和植物健康构成重大威胁，但机制尚不清楚。空间转录组学技术正在彻底改变我们对动物与寄生虫相互作用的理解，在微观尺度上揭示组织重组、细胞反应和感染动态。这些技术还加速了对治疗动物寄生虫感染的潜在靶点的识别。尽管具有潜力，但空间转录组学技术在植物-寄生虫相互作用中的应用是有限的。本文综述了空间转录组学在植物研究中的主要挑战。通过与动物系统的相似之处，我们讨论了空间转录组学如何有助于定位和识别效应物，揭示植物-寄生虫感染的分子机制，并找到新的疾病控制靶点。这一跨学科的观点为未来的植物和动物寄生虫学研究提供了路线图。

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引用次数: 0