Biotechnology advances最新文献_第3页

Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications 手性螺旋支架：释放其在生物分子相互作用和生物医学应用中的潜力。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-01-03 DOI: 10.1016/j.biotechadv.2024.108513

Ghada Bouz , Jaroslav Žádný , Jan Storch , Jan Vacek

In nature, various molecules possess spiral geometry. Such helical structures are even prevalent within the human body, represented classically by DNA and three-dimensional (secondary structure) protein folding. In this review, we chose helicenes and helicene-like structures –synthetically accessible carbon-rich molecules– as a compelling example of helically chiral scaffolds. Helicene chemistry, traditionally anchored in materials science, has been a subject of increasing interest in the biomedical field due to the unique optical and chiral properties of these helical structures. This review explores the diverse applications of helicenes in biomedicine, focusing on their role in cell imaging, protective coatings for implants, drug delivery systems, biosensors, and drug discovery. We discuss the unique properties of helicenes and helicene-like structures, highlighting their ability to form complex interactions with various biomolecules and their potential in the development of candidates for therapeutic agents. Recent advances in helicene derivatives with enhanced circularly polarized luminescence and other photochemical properties are also reviewed, underlining their utility in precise bio-imaging and diagnostic techniques. The review consolidates the current literature and emphasizes the growing importance of helicenes in bridging chemistry, materials science, and biology for innovative technological and biomedical applications.

在自然界中，各种分子都具有螺旋几何形状。这种螺旋结构甚至在人体内也很普遍，典型的代表是DNA和三维（二级结构）蛋白质折叠。在这篇综述中，我们选择螺旋烯和螺旋烯类结构-可合成的富碳分子-作为螺旋手性支架的一个引人注目的例子。螺旋烯化学，传统上扎根于材料科学，由于这些螺旋结构独特的光学和手性，已经成为生物医学领域越来越感兴趣的主题。本文综述了螺旋蛋白在生物医学中的应用，重点介绍了螺旋蛋白在细胞成像、植入物保护涂层、药物传递系统、生物传感器和药物发现等方面的作用。我们讨论了螺旋烯和螺旋烯类结构的独特性质，强调了它们与各种生物分子形成复杂相互作用的能力，以及它们在开发候选治疗剂方面的潜力。综述了具有增强圆偏振光和其他光化学性质的螺旋烯衍生物的最新进展，强调了它们在精确生物成像和诊断技术中的应用。这篇综述整合了目前的文献，并强调螺旋烯在连接化学、材料科学和生物学的创新技术和生物医学应用中的重要性。

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引用次数: 0

Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions 细菌糖蛋白工程的进展：对当前技术、新出现的挑战和未来方向的评述。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2025-01-02 DOI: 10.1016/j.biotechadv.2024.108514

Ziyu Li , Yujie Wang , Xiaojing Zhao , Qing Meng , Guozhen Ma , Lijie Xie , Xiaolong Jiang , Yutao Liu , Di Huang

Protein glycosylation, which involves the addition of carbohydrate chains to amino acid side chains, imparts essential properties to proteins, offering immense potential in synthetic biology applications. Despite its importance, natural glycosylation pathways present several limitations, highlighting the need for new tools to better understand glycan structures, recognition, metabolism, and biosynthesis, and to facilitate the production of biologically relevant glycoproteins. The field of bacterial glycoengineering has gained significant attention due to the ongoing discovery and study of bacterial glycosylation systems. By utilizing protein glycan coupling technology, a wide range of valuable glycoproteins for clinical and diagnostic purposes have been successfully engineered. This review outlines the recent advances in bacterial protein glycosylation from the perspective of synthetic biology and metabolic engineering, focusing on the development of new glycoprotein therapeutics and vaccines. We provide an overview of the production of high-value, customized glycoproteins using prokaryotic glycosylation platforms, with particular emphasis on four key elements: (i) glycosyltransferases, (ii) carrier proteins, (iii) glycosyl donors, and (iv) host bacteria. Optimization of these elements enables precise control over glycosylation patterns, thus enhancing the potential of the resulting products. Finally, we discuss the challenges and future prospects of leveraging synthetic biology technologies to develop microbial glyco-factories and cell-free systems for efficient glycoprotein production.

蛋白质糖基化是指在氨基酸侧链上添加碳水化合物链，赋予蛋白质基本特性，在合成生物学应用中具有巨大潜力。尽管它很重要，但天然糖基化途径存在一些局限性，强调需要新的工具来更好地理解糖基结构、识别、代谢和生物合成，并促进生物相关糖蛋白的产生。由于细菌糖基化系统的不断发现和研究，细菌糖工程领域受到了极大的关注。通过利用蛋白聚糖偶联技术，已经成功地设计了一系列具有临床和诊断用途的有价值的糖蛋白。本文从合成生物学和代谢工程的角度综述了近年来细菌蛋白糖基化的研究进展，重点介绍了新的糖蛋白治疗药物和疫苗的开发。我们概述了使用原核糖基化平台生产高价值定制糖蛋白的概况，特别强调了四个关键要素：(i)糖基转移酶，（ii）载体蛋白，（iii）糖基供体和（iv）宿主细菌。这些元素的优化可以精确控制糖基化模式，从而提高最终产物的潜力。最后，我们讨论了利用合成生物学技术开发微生物糖工厂和无细胞系统以高效生产糖蛋白的挑战和未来前景。

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引用次数: 0

Lignocellulosic biomass as promising substrate for polyhydroxyalkanoate production: Advances and perspectives 木质纤维素生物质作为生产聚羟基烷酸酯的有前途的底物：进展与展望。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-12-30 DOI: 10.1016/j.biotechadv.2024.108512

Dongna Li , Fei Wang , Xuening Zheng , Yingying Zheng , Xiaosen Pan , Jianing Li , Xiaojun Ma , Fen Yin , Qiang Wang

The depletion of fossil resources, coupled with global warming and adverse environmental impact of traditional petroleum-based plastics, have necessitated the discovery of renewable resources and innovative biodegradable materials. Lignocellulosic biomass (LB) emerges as a highly promising, sustainable and eco-friendly approach for accumulating polyhydroxyalkanoate (PHA), as it completely bypasses the problem of “competition for food”. This sustainable and economically efficient feedstock has the potential to lower PHA production costs and facilitate its competitive commercialization, and support the principles of circular bioeconomy. LB predominantly comprises cellulose, hemicellulose, and lignin, which can be converted into high-quality substrates for PHA production by various means. Future efforts should focus on maximizing the value derived from LB. This review highlights the momentous and valuable research breakthroughs in recent years, showcasing the biosynthesis of PHA using low-cost LB as a potential feedstock. The metabolic mechanism and pathways of PHA synthesis by microbes, as well as the key enzymes involved, are summarized, offering insights into improving microbial production capacity and fermentation metabolic engineering. Life cycle assessment and techno-economic analysis for sustainable and economical PHA production are introduced. Technological hurdles such as LB pretreatment, and performance limitations are highlighted for their impact on enhancing the sustainable production and application of PHA. Meanwhile, the development direction of co-substrate fermentation of LB and with other carbon sources, integrated processes development, and co-production strategies were also proposed to reduce the cost of PHA and effectively valorize wastes.

化石资源的枯竭，加上全球变暖和传统石油基塑料对环境的不利影响，有必要发现可再生资源和创新的生物降解材料。木质纤维素生物质（LB）是一种非常有前途的、可持续的、环保的聚羟基烷酸酯（PHA）积累方法，因为它完全绕过了“食物竞争”的问题。这种可持续和经济高效的原料具有降低PHA生产成本和促进其竞争性商业化的潜力，并支持循环生物经济原则。LB主要由纤维素、半纤维素和木质素组成，它们可以通过各种方式转化为PHA生产的高质量底物。未来的努力应集中在最大限度地发挥LB的价值上。本文综述了近年来重大和有价值的研究突破，展示了使用低成本LB作为潜在原料的PHA生物合成。综述了微生物合成PHA的代谢机制和途径，以及所涉及的关键酶，为提高微生物生产能力和发酵代谢工程提供参考。介绍了PHA可持续经济生产的生命周期评价和技术经济分析。LB预处理和性能限制等技术障碍对PHA可持续生产和应用的影响尤为突出。同时，提出了LB与其他碳源共底物发酵的发展方向、一体化工艺开发和协同生产策略，以降低PHA的成本，有效地实现废弃物的增值。

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引用次数: 0

Biohydrogen fermentation from pretreated biomass in lignocellulose biorefinery: Effects of inhibitory byproducts and recent progress in mitigation strategies 木质纤维素生物炼制中预处理生物质的生物氢发酵：抑制副产物的影响和缓解策略的最新进展。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-12-29 DOI: 10.1016/j.biotechadv.2024.108508

Zi-Tong Zhao , Shan-Shan Yang , Geng Luo , Han-Jun Sun , Bing-Feng Liu , Guang-Li Cao , Mei-Yi Bao , Ji-Wei Pang , Nan-Qi Ren , Jie Ding

Lignocellulosic biomass (LCB) is expected to play a critical role in achieving the goal of biomass-to-bioenergy conversion because of its wide distribution and low price. Biomass fermentation is a promising method for the sustainable generation of biohydrogen (bioH₂) from the renewable feedstock. Due to the inherent resistant structure of biomass, LCB needs to be pretreated to improve its digestibility and utilization. However, certain intermediates by-products generated during the pretreatment process, such as phenolic compounds, furan derivatives, and aldehydes, have been identified as potent inhibitors of subsequent anaerobic fermentation due to their disruptive effects on the physiological and metabolic functions of hydrogen-producing microbiota. To counteract the negative effects of these inhibitors on bio-H₂ fermentation, various detoxification strategies for LCB hydrolysates have been explored. This review presents a comprehensive analysis of fermentation-inhibitory by-products commonly generated by modern pretreatment protocols and their negative impacts on biohydrogen fermentation. Furthermore, the underlying mechanisms of inhibition upon hydrogen-producing microbes and their impacts on microbial community dynamics are exhibited. State-of-the-art strategies for detoxifying pretreated LCB have been also discussed, along with alternative pretreatment strategies designed to minimize or eliminate the formation of inhibitory by-products. Additionally, this review addresses the significant gap in the economic viability assessments of these processes, offering a detailed evaluation of both the technological and economic feasibility of biomass fermentation. Given the limitations of previous studies, strategies for cost-effective pretreatment and detoxification should be developed in the future to overcome the inhibition of fermentation inhibitors in the bioconversion of biomass to hydrogen.

木质纤维素生物质（LCB）由于其广泛的分布和低廉的价格，有望在实现生物质到生物能源转换的目标中发挥关键作用。生物质发酵是从可再生原料中可持续生产生物氢（bioH2）的一种很有前途的方法。由于生物质固有的抗性结构，需要对LCB进行预处理，以提高其消化率和利用率。然而，预处理过程中产生的某些中间体副产物，如酚类化合物、呋喃衍生物和醛类，已被确定为后续厌氧发酵的有效抑制剂，因为它们对产氢微生物群的生理和代谢功能具有破坏性影响。为了抵消这些抑制剂对生物- h2发酵的负面影响，人们探索了LCB水解物的各种解毒策略。本文综述了现代预处理方案中常见的发酵抑制副产物及其对生物氢发酵的负面影响。此外，还揭示了抑制产氢微生物的潜在机制及其对微生物群落动态的影响。最先进的策略解毒预处理LCB也已讨论，以及替代预处理策略，旨在尽量减少或消除抑制副产物的形成。此外，本综述解决了这些工艺在经济可行性评估方面的重大差距，提供了生物质发酵技术和经济可行性的详细评估。鉴于以往研究的局限性，未来应开发具有成本效益的预处理和解毒策略，以克服发酵抑制剂对生物质转化为氢的抑制作用。

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引用次数: 0

Carboxysomes: The next frontier in biotechnology and sustainable solutions 羧基体：生物技术和可持续解决方案的下一个前沿。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-12-26 DOI: 10.1016/j.biotechadv.2024.108511

Sulamita Santos Correa , Júnia Schultz , Brandon Zahodnik-Huntington , Andreas Naschberger , Alexandre Soares Rosado

Some bacteria possess microcompartments that function as protein-based organelles. Bacterial microcompartments (BMCs) sequester enzymes to optimize metabolic reactions. Several BMCs have been characterized to date, including carboxysomes and metabolosomes. Genomic analysis has identified novel BMCs and their loci, often including genes for signature enzymes critical to their function, but further characterization is needed to confirm their roles. Among the various BMCs, carboxysomes, which are found in cyanobacteria and some chemoautotrophic bacteria, and are most extensively investigated. These self-assembling polyhedral proteinaceous BMCs are essential for carbon fixation. Carboxysomes encapsulate the enzymes RuBisCo and carbonic anhydrase, which increase the carbon fixation rate in the cell and decrease the oxygenation rate by RuBisCo. The ability of carboxysomes to concentrate carbon dioxide in crops and industrially relevant microorganisms renders them attractive targets for carbon assimilation bioengineering. Thus, carboxysome characterization is the first step toward developing carboxysome-based applications. Therefore, this review comprehensively explores carboxysome morphology, physiology, and biochemistry. It also discusses recent advances in microscopy and complementary techniques for isolating and characterizing this versatile class of prokaryotic organelles.

有些细菌具有作为蛋白质细胞器的微室。细菌微室（BMCs）隔离酶以优化代谢反应。到目前为止，已经对几种bmc进行了表征，包括羧基体和代谢体。基因组分析已经确定了新的bmc及其位点，通常包括对其功能至关重要的特征酶的基因，但需要进一步的表征来确认它们的作用。在各种bmc中，羧酸体是蓝藻和一些趋化自养细菌中发现的，研究最广泛。这些自组装的多面体蛋白bmc对碳固定至关重要。羧小体包封RuBisCo和碳酸酐酶，增加细胞内的碳固定速率，降低RuBisCo的氧合速率。羧酶体在作物和工业相关微生物中浓缩二氧化碳的能力使它们成为碳同化生物工程的有吸引力的目标。因此，羧基体表征是开发基于羧基体的应用的第一步。因此，本文对羧基体的形态、生理和生物化学进行了全面的探讨。它还讨论了显微镜和互补技术的最新进展，以分离和表征这类多用途的原核细胞器。

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引用次数: 0

Advanced technological approaches and market status analysis of xylose bioconversion and utilization: Xylooligosacharides and xylonic acid as emerging products 木糖生物转化利用的先进技术途径及市场现状分析：低聚木糖和木糖酸是新兴产品。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-12-26 DOI: 10.1016/j.biotechadv.2024.108509

Jian Han , Faqiha Hamza , Jianming Guo , Mahmoud Sayed , Sang-Hyun Pyo , Yong Xu

The efficient conversion of xylose is a short board of cask effect to lignocellulosic biorefining, by markedly affecting the total economic and environmental benefits. Based on a comprehensive analysis of the current commercial status of traditional xylose utilization and industrial technology development, this review outlines new technological avenues for the efficient utilization of xylose from lignocellulosic biomass, focusing on super prebiotic xylo-oligosaccharides and multifunctional platform compound xylonic acid. Firstly, the traditional products that can be derived from lignocellulosic xylose, including xylitol (447.88 billion USD in 2022), furfural (662 million USD in 2023), and bioethanol (46.18 billion USD in 2022), are introduced along with the current market status and latest production technologies. Then, the discussion covers the industrial development and production methods of xylo-oligosaccharides, and highlights the potential of xylonic acid, focusing on innovative whole-cell catalysis in a sealed oxygen supply-bioreactor system. Finally, other directions for efficient and high-value utilization of lignocellulosic xylose are summarized, including lactic acid, succinic acid, and 2,3-butanediol. This review aims to provide new perspectives on the utilization and valorization of xylose by summarizing main traditional industrial products and emerging products, thereby promoting the development of the entire lignocellulosic biomass field.

木糖的高效转化是木桶效应对木质纤维素生物精制的一个短板，它显著地影响着整体经济效益和环境效益。在综合分析传统木糖利用的商业现状和工业技术发展的基础上，综述了木质纤维素生物质中木糖高效利用的新技术途径，重点介绍了超级益生元低聚木糖和多功能平台化合物木糖酸。首先介绍了木质纤维素木糖可衍生的传统产品，包括木糖醇（2022年为4478.8亿美元）、糠醛（2023年为6.62亿美元）和生物乙醇（2022年为461.8亿美元），以及市场现状和最新生产技术。然后，讨论了低聚木糖的工业发展和生产方法，并强调了木糖酸的潜力，重点介绍了密封供氧生物反应器系统中创新的全细胞催化。最后，总结了木质纤维素木糖高效、高价值利用的其他方向，包括乳酸、琥珀酸和2,3-丁二醇。本文旨在通过总结木糖的主要传统工业产品和新兴产品，为木糖的利用和增值提供新的视角，从而促进整个木质纤维素生物质领域的发展。

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引用次数: 0

Biocatalytic oxyfunctionalization of unsaturated fatty acids to oxygenated chemicals via hydroxy fatty acids 不饱和脂肪酸经羟基脂肪酸氧化功能化为含氧化学物质的生物催化。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-12-26 DOI: 10.1016/j.biotechadv.2024.108510

Deok-Kun Oh , Tae-Eui Lee , Jin Lee , Kyung-Chul Shin , Jin-Byung Park

The selective oxyfunctionalization of unsaturated fatty acids is difficult in chemical reactions, whereas regio- and stereoselective oxyfunctionalization is often performed in biocatalytic synthesis. Fatty acid oxygenases, including hydratases, lipoxygenases, dioxygenases, diol synthases, cytochrome P450 monooxygenases, peroxygenases, and 12-hydroxylases, are used to convert C16 and C18 unsaturated fatty acids to diverse regio- and stereoselective mono-, di-, and trihydroxy fatty acids via selective oxyfunctionalization. The formed hydroxy fatty acids or hydroperoxy fatty acids are metabolized to industrially important oxygenated chemicals such as lactones, green leaf volatiles, and bioplastic monomers, including ω-hydroxy fatty acids, α,ω-dicarboxylic acids, and fatty alcohols, by biocatalysts. For increased oxyfunctionalization of unsaturated fatty acids, enzyme engineering, functional and balanced expression in recombinant cells, selection of suitable catalyst types, and reaction engineering have been suggested. This review describes biocatalysts involved in the oxyfunctionalization of unsaturated fatty acids and the production of hydroxy fatty acids and oxygenated chemicals.

不饱和脂肪酸的选择性氧化官能化在化学反应中是困难的，而区域和立体选择性氧化官能化通常在生物催化合成中进行。脂肪酸加氧酶，包括水合酶、脂加氧酶、双加氧酶、二醇合成酶、细胞色素P450单加氧酶、过加氧酶和12-羟化酶，可通过选择性氧化功能化将C16和C18不饱和脂肪酸转化为多种区域和立体选择性的单、二、三羟基脂肪酸。形成的羟基脂肪酸或羟基过氧脂肪酸被生物催化剂代谢成工业上重要的含氧化学物质，如内酯、绿叶挥发物和生物塑料单体，包括ω-羟基脂肪酸、α、ω-二羧酸和脂肪醇。为了增加不饱和脂肪酸的氧化官能化，建议采用酶工程、在重组细胞中功能和平衡表达、选择合适的催化剂类型和反应工程。本文综述了不饱和脂肪酸的氧化官能化、生产羟基脂肪酸和含氧化合物的生物催化剂。

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引用次数: 0

HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing hek组学：利用组学优化重组腺相关病毒（rAAV）基因治疗生产的HEK293的前景。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-12-19 DOI: 10.1016/j.biotechadv.2024.108506

Sai Guna Ranjan Gurazada , Hannah M. Kennedy , Richard D. Braatz , Steven J. Mehrman , Shawn W. Polson , Irene T. Rombel

Gene therapy is poised to transition from niche to mainstream medicine, with recombinant adeno-associated virus (rAAV) as the vector of choice. However, robust, scalable, industrialized production is required to meet demand and provide affordable patient access, which has not yet materialized. Closing the chasm between demand and supply requires innovation in biomanufacturing to achieve the essential step change in rAAV product yield and quality. Omics provides a rich source of mechanistic knowledge that can be applied to HEK293, the most commonly used cell line for rAAV production. In this review, the findings from a growing number of diverse studies that apply genomics, epigenomics, transcriptomics, proteomics, and metabolomics to HEK293 bioproduction are explored. Learnings from CHO-omics, application of omics approaches to improve CHO bioproduction, provide a framework to explore the potential of “HEK-omics” as a multi-omics-informed approach providing actionable mechanistic insights for improved transient and stable production of rAAV and other recombinant products in HEK293.

随着重组腺相关病毒（rAAV）作为载体的选择，基因治疗正准备从利基医学过渡到主流医学。然而，需要强大的、可扩展的工业化生产来满足需求并为患者提供负担得起的治疗，这一点尚未实现。缩小需求和供应之间的鸿沟需要生物制造的创新，以实现rAAV产品产量和质量的基本步骤变化。组学提供了丰富的机制知识来源，可应用于HEK293，最常用的rAAV生产细胞系。本文综述了基因组学、表观基因组学、转录组学、蛋白质组学和代谢组学在HEK293生物生产中的应用。从CHO- omics中获得的经验和应用组学方法改善CHO生物生产，为探索“HEK-Omics”作为一种多组学方法的潜力提供了一个框架，为改善rAAV和HEK293中其他重组产品的瞬时和稳定生产提供了可行的机制见解。

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引用次数: 0

Terahertz scanning near-field optical microscopy for biomedical detection: Recent advances, challenges, and future perspectives 太赫兹扫描近场光学显微镜用于生物医学检测：最新进展、挑战和未来展望。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-12-19 DOI: 10.1016/j.biotechadv.2024.108507

Shihan Yan , Guanyin Cheng , Zhongbo Yang , Yuansen Guo , Ligang Chen , Ying Fu , Fucheng Qiu , Jonathan J. Wilksch , Tianwu Wang , Yiwen Sun , Junchao Fan , Xunbin Wei , Jiaguang Han , Fei Sun , Shixiang Xu , Huabin Wang

Terahertz (THz) radiation is widely recognized as a non-destructive, label-free, and highly- sensitive tool for biomedical detections. Nevertheless, its application in precision biomedical fields faces challenges due to poor spatial resolution caused by intrinsically long wavelength characteristics. THz scanning near-field optical microscopy (THz-SNOM), which surpasses the Rayleigh criterion, offers micrometer and nanometer-scale spatial resolution, making it possible to perform precise bioinspection with THz imaging. THz-SNOM is attracting considerable attention for its potential in advanced biomedical research and diagnosis. Currently, its family typically includes four members based on distinct principles, which are suitable for different biological applications. This review provides an overview of the principles of these THz-SNOM modalities, outlines their various applications, identifies the obstacles hindering their performance, and envisions their future development.

太赫兹（THz）辐射被广泛认为是一种非破坏性的、无标签的、高灵敏度的生物医学检测工具。然而，由于其固有的长波特性导致空间分辨率较差，在精密生物医学领域的应用面临挑战。太赫兹扫描近场光学显微镜（THz- snom），超过瑞利标准，提供微米和纳米尺度的空间分辨率，使其能够执行精确的生物检测与太赫兹成像。THz-SNOM因其在先进生物医学研究和诊断方面的潜力而引起了相当大的关注。目前，根据不同的原理，其家族通常包括四个成员，适用于不同的生物学应用。本文概述了这些THz-SNOM模式的原理，概述了它们的各种应用，确定了阻碍其性能的障碍，并展望了它们的未来发展。

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引用次数: 0

The role of the proteosurfaceome and exoproteome in bacterial coaggregation 蛋白质表面组和外蛋白质组在细菌共聚集中的作用。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-12-16 DOI: 10.1016/j.biotechadv.2024.108505

Ana C. Afonso , Maria J. Saavedra , Manuel Simões , Lúcia C. Simões

Bacterial coaggregation is a critical process in multispecies biofilm formation, driven by specific molecular interactions that facilitate the adhesion and aggregation of bacterial cells. These interactions are essential for the development and persistence of complex microbial communities. This review provides a comprehensive analysis of the roles of the proteosurfaceome and exoproteome in bacterial coaggregation. The proteosurfaceome, comprising surface-bound molecules such as adhesins, drives species-specific interactions crucial for partner recognition and adhesion. In parallel, the exoproteome, particularly extracellular polymeric substances (EPS), enhances aggregate stability by reinforcing structural integrity and facilitating intercellular communication, although its direct role in coaggregation remains to be fully clarified. By integrating these perspectives, this review aims to elucidate how the proteosurfaceome and exoproteome influence bacterial coaggregation, offering insights into their combined impact on microbial community structure and function. Furthermore, we highlight existing knowledge gaps and propose directions for future research.

细菌共聚集是多物种生物膜形成的关键过程，由促进细菌细胞粘附和聚集的特定分子相互作用驱动。这些相互作用对于复杂微生物群落的发展和持续是必不可少的。本文综述了蛋白质表面组和外蛋白质组在细菌共聚集中的作用。蛋白质表面体，包括表面结合的分子，如粘附素，驱动物种特异性相互作用，对伴侣识别和粘附至关重要。与此同时，外蛋白质组，特别是细胞外聚合物（EPS），通过加强结构完整性和促进细胞间通讯来增强聚集体的稳定性，尽管其在共聚集中的直接作用仍有待完全阐明。通过整合这些观点，本文旨在阐明蛋白质表面组和外蛋白质组如何影响细菌共聚集，并为它们对微生物群落结构和功能的综合影响提供见解。此外，我们强调了现有的知识差距，并提出了未来的研究方向。

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