Biotechnology advances最新文献_第2页

Implications of glycosylation for the development of selected cytokines and their derivatives for medical use 糖基化对开发用于医疗用途的特定细胞因子及其衍生物的影响。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-10-22 DOI: 10.1016/j.biotechadv.2024.108467

Giulia Scapin , Ece Cagdas , Lise Marie Grav , Nathan E Lewis , Steffen Goletz , Lise Hafkenscheid

Cytokines are important regulators of immune responses, making them attractive targets for autoimmune diseases and cancer therapeutics. Yet, the significance of cytokine glycosylation remains underestimated. Many cytokines carry N- and O-glycans and some even undergo C-mannosylation. Recombinant cytokines produced in heterologous host cells may lack glycans or exhibit a different glycosylation pattern such as varying levels of galactosylation, sialylation, fucosylation or xylose addition compared to their human counterparts, potentially impacting critical immune interactions.

We focused on cytokines that are currently utilized or designed in advanced therapeutic formats, including immunocytokines, fusokines, engager cytokines, and genetically engineered ‘supercytokines.’ Despite the innovative designs of these cytokine derivatives, their glycosylation patterns have not been extensively studied. By examining the glycosylation of the human native cytokines, G-CSF and GM-CSF, interferons β and γ, TNF-α and interleukins-2, −3 -4, −6, −7, −9, −12, −13, −15, −17A, −21, and − 22, we aim to assess its potential impact on their therapeutic derivatives. Understanding the glycosylation of the native cytokines could provide critical insights into the safety, efficacy, and functionality of these next-generation cytokine therapies, affecting factors such as stability, bioactivity, antigenicity, and half-life. This knowledge can guide the choice of optimal expression hosts for production and advance the development of effective cytokine-based therapeutics and synthetic immunology drugs.

细胞因子是免疫反应的重要调节因子，使其成为自身免疫性疾病和癌症治疗的诱人靶点。然而，细胞因子糖基化的重要性仍被低估。许多细胞因子都带有 N-和 O-糖基，有些甚至会发生 C-甘露糖基化。在异源宿主细胞中产生的重组细胞因子可能缺乏聚糖，或表现出不同的糖基化模式，如与人类细胞因子相比，不同程度的半乳糖基化、硅氨酰化、岩藻糖基化或木糖添加，从而可能影响关键的免疫相互作用。我们重点研究了目前正在使用或设计用于高级治疗的细胞因子，包括免疫细胞因子、免疫细胞因子、吸引细胞因子和基因工程 "超级细胞因子"。尽管这些细胞因子衍生物设计新颖，但它们的糖基化模式还没有得到广泛研究。通过研究人类原生细胞因子、G-CSF 和 GM-CSF、干扰素 β 和 γ、TNF-α 以及白细胞介素-2、-3、-4、-6、-7、-9、-12、-13、-15、-17A、-21 和-22 的糖基化，我们旨在评估它们对其治疗衍生物的潜在影响。了解原生细胞因子的糖基化可为这些下一代细胞因子疗法的安全性、有效性和功能性提供重要的见解，这些见解会影响稳定性、生物活性、抗原性和半衰期等因素。这些知识可以指导选择最佳的表达宿主进行生产，并推动基于细胞因子的有效疗法和合成免疫学药物的开发。

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引用次数: 0

Constructed wetland microbial fuel cell as enhancing pollutants treatment technology to produce green energy 建造湿地微生物燃料电池作为提高污染物处理技术的绿色能源。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-10-20 DOI: 10.1016/j.biotechadv.2024.108468

Iryna Rusyn , Julio César Gómora-Hernández

The persistent challenge of water pollution, exacerbated by slow progress in ecofriendly technologies and accumulating pollutants, underscores the need for innovative solutions. Constructed Wetland Microbial Fuel Cell (CW-MFC) emerges as an intriguing environmental technology capable of adressing this issue by eliminating contaminants from wastewater while simultaneously producing green energy as an additional bonus. In recent years, CW-MFC technology has gained attention due to its sustainability and promising prospects for a circular waste-free industry. However, due to various technological and biological challenges, it has not yet achieved wide-scale application. This review examines the current state of CW-MFC technology and identifies both biotic and abiotic strategies for optimization through operational and structural improvements affecting biocomponents. Our review highlights several key findings: (1) Plants play an important role in reducing the system's inner resistance through mechanisms such as radial oxygen loss, evapotranspiration, and high photosynthetic flow, which facilitate electroactive bacteria and affect redox potential. (2) Plant characteristics such as root porosity, phloem and aerenchyma development, chlorophyll content, and plant biomass are key indicators of CW-MFC performance and significantly impact both pollutant removal and energy harvesting. (3) We expand the criteria for selecting suitable plants to include mesophytes and C3 pollutant-tolerant species, in addition to traditional aquatic and C4 plants. Additionally, the review presents several technical approaches that enhance CW-MFC efficiency: (1) design optimization, (2) use of novel materials, and (3) application of external electrical fields, aeration, light, and temperature adjustments. CW-MFCs are capable of nearly complete elimination of a wide range of contaminants, including organic matter (84 % ± 10), total nitrogen (80 % ± 7) and phosphorus (79 % ± 18) compounds, metals (86 % ± 10), pharmaceuticals (87 % ± 7), dyes (90 % ± 8), and other complex pollutants, while generating green energy. We hope our findings will be useful in optimizing CW-MFC design and providing insights for researchers aiming to advance the technology and facilitate its future scaling.

由于环保技术进展缓慢和污染物不断累积，水污染这一长期挑战变得更加严峻，这凸显了对创新解决方案的需求。构筑湿地微生物燃料电池（CW-MFC）是一项引人关注的环保技术，它能够消除废水中的污染物，同时还能生产绿色能源。近年来，CW-MFC 技术因其可持续性和无废物循环工业的美好前景而备受关注。然而，由于面临各种技术和生物挑战，该技术尚未实现大规模应用。本综述研究了 CW-MFC 技术的现状，并通过对生物组件的操作和结构改进，确定了生物和非生物优化策略。我们的综述强调了几个重要发现：（1）植物通过径向氧损失、蒸腾作用和高光合作用流量等机制，在降低系统内阻方面发挥着重要作用，这有利于电活性细菌的生长并影响氧化还原电位。(2）根系孔隙率、韧皮部和气生根发育、叶绿素含量和植物生物量等植物特征是 CW-MFC 性能的关键指标，对污染物去除和能量收集都有显著影响。(3）我们扩大了选择合适植物的标准，除了传统的水生植物和 C4 植物外，还包括中生植物和 C3 耐污染物种。此外，综述还介绍了几种提高 CW-MFC 效率的技术方法：（1）优化设计；（2）使用新型材料；（3）应用外部电场、曝气、光照和温度调节。CW-MFC 几乎能完全消除各种污染物，包括有机物（84 % ± 10）、总氮（80 % ± 7）和磷（79 % ± 18）化合物、金属（86 % ± 10）、药物（87 % ± 7）、染料（90 % ± 8）以及其他复杂污染物，同时还能产生绿色能源。我们希望我们的研究结果将有助于优化 CW-MFC 的设计，并为旨在推动该技术发展和促进其未来推广的研究人员提供见解。

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引用次数: 0

Exploring the versatility of Porphyridium sp.: A comprehensive review of cultivation, bio-product extraction, purification, and characterization techniques 探索卟啉藻的多功能性：培养、生物产品提取、纯化和表征技术综述。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-10-20 DOI: 10.1016/j.biotechadv.2024.108471

Anh Quynh Nguyen , Maedeh Mohammadi , Mahsa Alian , Gangatharan Muralitharan , Vikas Singh Chauhan , Venkatesh Balan

Interest in red microalgae of the Porphyridium genus has surged due to their richness in phycobiliproteins, polyunsaturated fatty acids, and sulfated polysaccharides. These biomasses and their derivatives find applications across food, feed, nutraceutical, pharmaceutical, and cosmetic industries. A deeper understanding of their properties and extraction methods is essential to optimize downstream processing. This paper comprehensively reviews Porphyridium sp., focusing on cultivation techniques, bioproduct extraction, purification, and characterization. It delves into protein, lipid, and polysaccharide extraction, considering the influence of culture conditions on biomass yield. Various methods like chromatography, electrophoresis, and membrane-based techniques for cell lysis and bioproduct recovery are explored, highlighting their pros and cons. By offering diverse insights, this review aims to inspire innovative research and industry progress in red microalgae biotechnology, contributing to sustainable solutions across sectors.

由于富含藻胆蛋白、多不饱和脂肪酸和硫酸化多糖，人们对卟啉属红色微藻的兴趣大增。这些生物质及其衍生物可应用于食品、饲料、保健品、制药和化妆品行业。深入了解它们的特性和提取方法对于优化下游加工过程至关重要。本文全面回顾了卟啉藻，重点介绍了培养技术、生物产品提取、纯化和表征。考虑到培养条件对生物质产量的影响，本文深入探讨了蛋白质、脂质和多糖的提取。还探讨了用于细胞裂解和生物产品回收的各种方法，如色谱法、电泳法和基于膜的技术，并强调了它们的优缺点。本综述通过提供不同的见解，旨在激励红色微藻生物技术领域的创新研究和行业进步，为各行各业的可持续解决方案做出贡献。

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引用次数: 0

Process simulation and evaluation of scaled-up biocatalytic systems: Advances, challenges, and future prospects 放大生物催化系统的过程模拟和评估：进展、挑战和未来展望。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-10-20 DOI: 10.1016/j.biotechadv.2024.108470

Zhonghao Chen , Lei Wang

With the increased demand for bio-based products and the rapid development of biomanufacturing technologies, biocatalytic reactions including microorganisms and enzyme based, have become promising approaches. Prior to the scale-up of production process, environmental and economic feasibility analysis are essential for the development of a sustainable and intelligent bioeconomy in the context of industry 4.0. To achieve these goals, process simulation supports system optimization, improves energy and resource utilization efficiencies, and supports digital bioprocessing. However, due to the insufficient understanding of cellular metabolism and interaction mechanisms, there is still a lack of rational and transparent simulation tools to efficiently simulate, control, and optimize microbial/enzymatic reaction processes. Therefore, there is an urgent need to develop frameworks that integrate kinetic modeling, process simulation, and sustainability analysis for bioreaction simulations and their optimization. This review summarizes and compares the advantages and disadvantages of different process simulation software and models in simulating biocatalytic processes, identifies the limitations of traditional reaction kinetics models, and proposes the requirement of simulations close to real reactions. In addition, we explore the current state of kinetic modeling at the microscopic scale and how process simulation can be linked to kinetic models of cellular metabolism and computational fluid dynamics modeling. Finally, this review discusses the requirement of sensitivity analysis and how machine learning can assist with optimization of simulations to improve energy efficiency and product yields from techno-economic and life cycle assessment perspectives.

随着对生物基产品需求的增加和生物制造技术的快速发展，包括微生物和酶在内的生物催化反应已成为前景广阔的方法。在扩大生产工艺规模之前，环境和经济可行性分析对于在工业 4.0 背景下发展可持续的智能生物经济至关重要。为实现这些目标，工艺模拟可支持系统优化，提高能源和资源利用效率，并支持数字化生物工艺。然而，由于对细胞新陈代谢和相互作用机制的认识不足，目前仍缺乏合理、透明的模拟工具来有效模拟、控制和优化微生物/酶反应过程。因此，亟需为生物反应模拟及其优化开发集成动力学建模、过程模拟和可持续性分析的框架。本综述总结并比较了不同过程模拟软件和模型在模拟生物催化过程中的优缺点，指出了传统反应动力学模型的局限性，并提出了模拟接近真实反应的要求。此外，我们还探讨了微观尺度动力学建模的现状，以及如何将过程模拟与细胞代谢动力学模型和计算流体动力学模型联系起来。最后，本综述讨论了敏感性分析的要求，以及机器学习如何从技术经济和生命周期评估的角度协助优化模拟，以提高能效和产品产量。

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引用次数: 0

Utilizing stimuli-responsive nanoparticles to deliver and enhance the anti-tumor effects of bilirubin 利用刺激响应型纳米粒子传递和增强胆红素的抗肿瘤效果。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-10-18 DOI: 10.1016/j.biotechadv.2024.108469

Elaheh Mirhadi , Alexandra E. Butler , Prashant Kesharwani , Amirhossein Sahebkar

Bilirubin (BR) is among the most potent endogenous antioxidants that originates from the heme catabolic pathway. Despite being considered as a dangerous and cytotoxic waste product at high concentrations, BR has potent antioxidant effects leading to the reduction of oxidative stress and inflammation, which play an important role in the development and progression of cancer. The purpose of this study is to introduce PEGylated BR nanoparticles (NPs), themselves or in combination with other anti-cancer agents. BR is effective when loaded into various nanoparticles and used in cancer therapy. Interestingly, BRNPs can be manipulated to create stimuli-responsive carriers providing a sustained and controlled, as well as on-demand, release of drug in response to internal or external factors such as reactive oxygen species, glutathione, light, enzymes, and acidic pH. This review suggests that BRNPs have the potential as tumor microenvironment-responsive delivery systems for effective targeting of various types of cancers.

胆红素（BR）是源自血红素分解途径的最有效的内源性抗氧化剂之一。尽管胆红素在高浓度时被认为是一种危险的细胞毒性废物，但它具有非凡的抗氧化作用，可减少氧化应激和炎症，而氧化应激和炎症在癌症的发生和发展中起着重要作用。本研究的目的是引入聚乙二醇化 BR 纳米粒子（NPs）本身或与其他抗癌剂结合使用。在癌症治疗中，BR 被载入各种纳米颗粒后，效果显著。有趣的是，BRNPs 可被操纵以创建刺激响应载体，从而根据活性氧、谷胱甘肽、光、酶和酸性 pH 值等内部或外部因素，提供持续、可控和按需的药物释放。综上所述，BRNPs 具有作为肿瘤微环境响应型递送系统的潜力，可有效针对各种癌症。

引用次数: 0

Exploring the landscape of FRET-based molecular sensors: Design strategies and recent advances in emerging applications 探索基于 FRET 的分子传感器：新兴应用领域的设计策略和最新进展。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-10-16 DOI: 10.1016/j.biotechadv.2024.108466

Neha Soleja, Mohd. Mohsin

Probing biological processes in living organisms that could provide one-of-a-kind insights into real-time alterations of significant physiological parameters is a formidable task that calls for specialized analytic devices. Classical biochemical methods have significantly aided our understanding of the mechanisms that regulate essential biological processes. These methods, however, are typically insufficient for investigating transient molecular events since they focus primarily on the end outcome. Fluorescence resonance energy transfer (FRET) microscopy is a potent tool used for exploring non-invasively real-time dynamic interactions between proteins and a variety of biochemical signaling events using sensors that have been meticulously constructed. Due to their versatility, FRET-based sensors have enabled the rapid and standardized assessment of a large array of biological variables, facilitating both high-throughput research and precise subcellular measurements with exceptional temporal and spatial resolution. This review commences with a brief introduction to FRET theory and a discussion of the fluorescent molecules that can serve as tags in different sensing modalities for studies in chemical biology, followed by an outlining of the imaging techniques currently utilized to quantify FRET highlighting their strengths and shortcomings. The article also discusses the various donor-acceptor combinations that can be utilized to construct FRET scaffolds. Specifically, the review provides insights into the latest real-time bioimaging applications of FRET-based sensors and discusses the common architectures of such devices. There has also been discussion of FRET systems with multiplexing capabilities and multi-step FRET protocols for use in dual/multi-analyte detections. Future research directions in this exciting field are also mentioned, along with the obstacles and opportunities that lie ahead.

探测生物体内的生物过程是一项艰巨的任务，需要专门的分析设备才能对重要生理参数的实时变化提供独到的见解。经典的生物化学方法极大地帮助了我们对重要生物过程调控机制的理解。然而，这些方法通常不足以研究瞬时分子事件，因为它们主要关注的是最终结果。荧光共振能量转移（FRET）显微镜是一种有效的工具，可利用精心构建的传感器，非侵入式地探索蛋白质之间的实时动态相互作用和各种生化信号事件。由于其多功能性，基于 FRET 的传感器能够对大量生物变量进行快速、标准化的评估，促进了高通量研究和具有卓越时空分辨率的精确亚细胞测量。这篇综述首先简要介绍了 FRET 理论，讨论了在化学生物学研究的不同传感模式中可用作标记的荧光分子，然后概述了目前用于量化 FRET 的成像技术，重点介绍了这些技术的优势和不足。文章还讨论了可用于构建 FRET 支架的各种供体-受体组合。具体来说，这篇综述深入介绍了基于 FRET 的传感器的最新实时生物成像应用，并讨论了此类设备的常见架构。此外，还讨论了具有复用功能的 FRET 系统和用于双/多分析物检测的多步骤 FRET 协议。此外，还提到了这一令人兴奋的领域未来的研究方向，以及面临的障碍和机遇。

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引用次数: 0

An overview of biomethanation and the use of membrane technologies as a candidate to overcome H2 mass transfer limitations 生物甲烷化概述和膜技术作为克服 H2 传质限制的候选技术的使用

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-10-14 DOI: 10.1016/j.biotechadv.2024.108465

Manuel Fachal-Suárez , Santhana Krishnan , Sumate Chaiprapat , Daniel González , David Gabriel

Energy produced from renewable sources such as sun or wind are intermittent, depending on circumstantial factors. This fact explains why energy supply and demand do not match. In this context, the interest in biomethanation has increased as an interesting contribution to the Power-to-gas concept (P2G), transforming the extra amount of produced electricity into methane (CH₄). The reaction between green hydrogen (H₂) (produced by electrolysis) and CO₂ (pollutant present in biogas) can be catalysed by different microorganisms to produce biomethane, that can be injected into existing natural gas grid if reaching the standards. Thus, energy storage for both hydrogen and electricity, as well as transportation problems would be solved. However, H₂ diffusion to the liquid phase for its further biological conversion is the main bottleneck due to the low solubility of H₂. This review includes the state-of-the-art in biological hydrogenotrophic methanation (BHM) and membrane-based technologies. Specifically, the use of hollow-fiber membrane bioreactors as a technology to overcome H₂ diffusion limitations is reviewed. Furthermore, the influence of operating conditions, microbiology, H₂ diffusion and H₂ injection methods are critically discussed before setting the main recommendations about BHM.

太阳或风能等可再生能源产生的能源是间歇性的，取决于环境因素。这就是能源供需不匹配的原因所在。在这种情况下，人们对生物甲烷化的兴趣与日俱增，因为它是 "电转气 "概念（P2G）的一个有趣贡献，可将额外生产的电力转化为甲烷（CH4）。绿色氢气（H2）（由电解产生）和二氧化碳（沼气中的污染物）之间的反应可由不同的微生物催化产生生物甲烷，如果达到标准，可注入现有的天然气网。这样，氢气和电力的储能以及运输问题都将得到解决。然而，由于 H2 的溶解度低，H2 扩散到液相进行进一步生物转化是主要瓶颈。本综述包括生物养氢甲烷化（BHM）和膜技术的最新进展。具体而言，综述了使用中空纤维膜生物反应器作为克服 H2 扩散限制的技术。此外，在提出有关 BHM 的主要建议之前，还批判性地讨论了操作条件、微生物学、H2 扩散和 H2 注入方法的影响。

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引用次数: 0

The current progress of tandem chemical and biological plastic upcycling 目前化学和生物串联塑料升级再循环技术的进展情况

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-10-10 DOI: 10.1016/j.biotechadv.2024.108462

Yifeng Hu , Yuxin Tian , Chenghao Zou , Tae Seok Moon

Each year, millions of tons of plastics are produced for use in such applications as packaging, construction, and textiles. While plastic is undeniably useful and convenient, its environmental fate and transport have raised growing concerns about waste and pollution. However, the ease and low cost of producing virgin plastic have so far made conventional plastic recycling economically unattractive. Common contaminants in plastic waste and shortcomings of the recycling processes themselves typically mean that recycled plastic products are of relatively low quality in some cases. The high cost and high energy requirements of typical recycling operations also reduce their economic benefits. In recent years, the bio-upcycling of chemically treated plastic waste has emerged as a promising alternative to conventional plastic recycling. Unlike recycling, bio-upcycling uses relatively mild process conditions to economically transform pretreated plastic waste into value-added products. In this review, we first provide a précis of the general methodology and limits of conventional plastic recycling. Then, we review recent advances in hybrid chemical/biological upcycling methods for different plastics, including polyethylene terephthalate, polyurethane, polyamide, polycarbonate, polyethylene, polypropylene, polystyrene, and polyvinyl chloride. For each kind of plastic, we summarize both the pretreatment methods for making the plastic bio-available and the microbial chassis for degrading or converting the treated plastic waste to value-added products. We also discuss both the limitations of upcycling processes for major plastics and their potential for bio-upcycling.

每年都有数百万吨塑料被生产出来，用于包装、建筑和纺织等领域。虽然塑料的实用性和便利性毋庸置疑，但其对环境的影响以及运输过程中的浪费和污染问题却日益引起人们的关注。然而，由于原生塑料易于生产且成本低廉，迄今为止，传统的塑料回收在经济上并不具有吸引力。塑料废弃物中常见的污染物和回收工艺本身的缺陷通常意味着回收的塑料产品在某些情况下质量相对较低。典型的回收操作成本高、能耗大，也降低了其经济效益。近年来，对经过化学处理的塑料废弃物进行生物升级再循环已成为替代传统塑料回收利用的一种很有前途的方法。与回收不同的是，生物升级再循环利用相对温和的工艺条件，以经济的方式将预处理过的塑料废弃物转化为高附加值产品。在本综述中，我们首先简要介绍了传统塑料回收的一般方法和局限性。然后，我们回顾了针对不同塑料（包括聚对苯二甲酸乙二酯、聚氨酯、聚酰胺、聚碳酸酯、聚乙烯、聚丙烯、聚苯乙烯和聚氯乙烯）的化学/生物混合升级再循环方法的最新进展。对于每种塑料，我们都总结了使塑料具有生物可利用性的预处理方法，以及降解或将处理后的塑料废物转化为高附加值产品的微生物底盘。我们还讨论了主要塑料升级再循环工艺的局限性及其生物升级再循环的潜力。

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引用次数: 0

From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects 从木质纤维素生物质到用于可持续生物制造的单细胞油：当前进展与前景。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-10-09 DOI: 10.1016/j.biotechadv.2024.108460

Yu Duan , Limei Chen , Longxue Ma , Farrukh Raza Amin , Yida Zhai , Guofu Chen , Demao Li

As global temperatures rise and arid climates intensify, the reserves of Earth's resources and the future development of humankind are under unprecedented pressure. Traditional methods of food production are increasingly inadequate in meeting the demands of human life while remaining environmentally sustainable and resource-efficient. Consequently, the sustainable supply of lipids is expected to become a pivotal area for future food development. Lignocellulose biomass (LB), as the most abundant and cost-effective renewable resource, has garnered significant attention from researchers worldwide. Thus, bioprocessing based on LB is appearing as a sustainable model for mitigating the depletion of energy reserves and reducing carbon footprints. Currently, the transformation of LB primarily focuses on producing biofuels, such as bioethanol, biobutanol, and biodiesel, to address the energy crisis. However, there are limited reports on the production of single cell oil (SCO) from LB. This review, therefore, provides a comprehensive summary of the research progress in lignocellulosic pretreatment. Subsequently, it describes how the capability for lignocellulosic use can be conferred to cells through genetic engineering. Additionally, the current status of saccharification and fermentation of LB is outlined. The article also highlights the advances in synthetic biology aimed at driving the development of oil-producing microorganism (OPM), including genetic transformation, chassis modification, and metabolic pathway optimization. Finally, the limitations currently faced in SCO production from straw are discussed, and future directions for achieving high SCO yields from various perspectives are proposed. This review aims to provide a valuable reference for the industrial application of green SCO production.

随着全球气温的升高和干旱气候的加剧，地球资源的储备和人类未来的发展面临着前所未有的压力。传统的粮食生产方式越来越无法在满足人类生活需求的同时保持环境可持续性和资源效率。因此，脂质的可持续供应有望成为未来粮食发展的一个关键领域。木质纤维素生物质（LB）作为最丰富、最具成本效益的可再生资源，已引起全球研究人员的极大关注。因此，基于木质纤维素的生物加工正在成为缓解能源储备枯竭和减少碳足迹的可持续模式。目前，枸杞的转化主要集中在生产生物燃料，如生物乙醇、生物丁醇和生物柴油，以应对能源危机。然而，有关从枸杞中生产单细胞油（SCO）的报道却很有限。因此，本综述全面总结了木质纤维素预处理的研究进展。随后，它介绍了如何通过基因工程赋予细胞利用木质纤维素的能力。此外，文章还概述了枸杞糖化和发酵的现状。文章还重点介绍了旨在推动产油微生物（OPM）发展的合成生物学进展，包括基因转化、底盘改造和代谢途径优化。最后，讨论了目前利用秸秆生产 SCO 所面临的限制，并从不同角度提出了实现 SCO 高产的未来方向。本综述旨在为绿色 SCO 生产的工业应用提供有价值的参考。

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引用次数: 0

The new chassis in the flask: Advances in Vibrio natriegens biotechnology research 烧瓶中的新底盘：天然弧菌生物技术研究进展。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances

Pub Date : 2024-10-09 DOI: 10.1016/j.biotechadv.2024.108464

Matthew Lima , Charandatta Muddana , Zhengyang Xiao , Anindita Bandyopadhyay , Pramod P. Wangikar , Himadri B. Pakrasi , Yinjie J. Tang

Biotechnology has been built on the foundation of a small handful of well characterized and well-engineered organisms. Recent years have seen a breakout performer gain attention as a new entrant into the bioengineering toolbox: Vibrio natriegens. This review covers recent research efforts into making V. natriegens a biotechnology platform, using a large language model (LLM) and knowledge graph to expedite the literature survey process. Scientists have made advancements in research pertaining to the fundamental metabolic characteristics of V. natriegens, development and characterization of synthetic biology tools, systems biology analysis and metabolic modeling, bioproduction and metabolic engineering, and microbial ecology. Each of these subcategories has relevance to the future of V. natriegens for bioengineering applications. In this review, we cover these recent advancements and offer context for the impact they may have on the field, highlighting benefits and drawbacks of using this organism. From examining the recent bioengineering research, it appears that V. natriegens is on the precipice of becoming a platform bacterium for the future of biotechnology.

生物技术一直是建立在少数特征鲜明、工程技术完善的生物体基础之上的。近年来，生物工程工具箱中出现了一种新的生物，它的表现备受瞩目：纳氏弧菌。本综述介绍了将纳氏弧菌打造成生物技术平台的最新研究成果，其中使用了大型语言模型（LLM）和知识图谱来加快文献调查过程。科学家们在与 V. natriegens 的基本代谢特征、合成生物学工具的开发和特征描述、系统生物学分析和代谢建模、生物生产和代谢工程以及微生物生态学有关的研究方面取得了进展。这些子类别中的每一个都与未来 V. natriegens 在生物工程方面的应用有关。在这篇综述中，我们将介绍这些最新进展，并就它们可能对该领域产生的影响提供背景资料，同时强调使用这种生物体的好处和缺点。从最近的生物工程研究来看，V. natriegens 即将成为未来生物技术的平台菌。

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引用次数: 0