Pub Date : 2022-08-01DOI: 10.21776/ub.biotropika.2022.010.02.07
Anak Agung Gede Indraningrat, Pande Putu Christine Putri Purnami, Ida Bagus Gede Darmayasa
{"title":"Antibacterial Activity Screening Of Bacterial Isolates Associated With Seaweed Eucheuma cottonii From Coastal Area In Buleleng, Bali","authors":"Anak Agung Gede Indraningrat, Pande Putu Christine Putri Purnami, Ida Bagus Gede Darmayasa","doi":"10.21776/ub.biotropika.2022.010.02.07","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2022.010.02.07","url":null,"abstract":"","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89930556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-01DOI: 10.21776/ub.biotropika.2022.010.02.04
R. Rismawati, Yoga Dwi Jatmiko, S. Widyarti
{"title":"Antibacterial Activity of Pluchea indica Leaf Extract was Increased After Being Fermented with Saccharomyces cerevisiae and Added with its Cell-Free Supernatant","authors":"R. Rismawati, Yoga Dwi Jatmiko, S. Widyarti","doi":"10.21776/ub.biotropika.2022.010.02.04","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2022.010.02.04","url":null,"abstract":"","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77428739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-01DOI: 10.21776/ub.biotropika.2022.010.02.10
L. Purnamasari, Maria Victoria Carolino, Joseph F. dela Cruz
{"title":"The Antibacterial Properties of Psidium guajava Leaf Extract as a Wound Healing Agent of Laboratory Animals: a Review","authors":"L. Purnamasari, Maria Victoria Carolino, Joseph F. dela Cruz","doi":"10.21776/ub.biotropika.2022.010.02.10","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2022.010.02.10","url":null,"abstract":"","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82375273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-01DOI: 10.21776/ub.biotropika.2022.010.02.06
Fajar Husen, S.Si., M.Si., Nuniek Ina Ratnaningtyas
{"title":"Inhibitory Test of Gentamicin Antibiotics Against Escherichia coli and Staphylococcus aureus Bacteria Using Disc Method","authors":"Fajar Husen, S.Si., M.Si., Nuniek Ina Ratnaningtyas","doi":"10.21776/ub.biotropika.2022.010.02.06","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2022.010.02.06","url":null,"abstract":"","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89793271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-30DOI: 10.21776/ub.biotropika.2022.010.01.01
Marina Ratte, Irmanida Batubara, Y. Lestari
Xylocarpus granatum produces various bioactive compounds with diverse biological activities, one of them as an antioxidant. Endophytic actinobacteria can also produce bioactive compounds. This study aimed to explore the existence of the endophytic actinobacteria of X. granatum fruit and determine its antioxidant activity. The research method was carried out by isolating the endophytic actinobacteria from the fruit using selective media Humic Acid (HV). Furthermore, characterizing the obtained isolates using four different media, namely YSA, ISP2, ISP3, and ISP4, measured antioxidant activity for actinobacterial supernatants and actinobacterial ethyl acetate extracts using the DPPH method. The results showed that from X. granatum, 15 isolates of endophytic actinobacteria were obtained from fruit, seeds, and leaves. Endophytic actinobacteria isolates from X. granatum showed different colors of aerial mycelium, substrate mycelium, and spore chain type. XGF11, XGF12, and XGF4 isolates produced high antioxidant activity of the supernatant; 57.30%, 49.77%, and 41.90% inhibition, respectively. The antioxidant capacity of XGF12 ethyl acetate extract was 501.60 μg AEAC/mg extract and 465.47 μg AEAC/mg extract for XGF11 ethyl acetate extract. Based on 16S rRNA gene analysis, isolates XGF12 and XGF11 showed similarities with Streptomyces xylanilyticus, and isolated XGF4 showed similarities with Brevibacterium sediminis.
{"title":"Morphological Characterization and Antioxidant Activity of Actinobacteria from Xylocarpus granatum Growing in Mangrove Habitat","authors":"Marina Ratte, Irmanida Batubara, Y. Lestari","doi":"10.21776/ub.biotropika.2022.010.01.01","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2022.010.01.01","url":null,"abstract":"Xylocarpus granatum produces various bioactive compounds with diverse biological activities, one of them as an antioxidant. Endophytic actinobacteria can also produce bioactive compounds. This study aimed to explore the existence of the endophytic actinobacteria of X. granatum fruit and determine its antioxidant activity. The research method was carried out by isolating the endophytic actinobacteria from the fruit using selective media Humic Acid (HV). Furthermore, characterizing the obtained isolates using four different media, namely YSA, ISP2, ISP3, and ISP4, measured antioxidant activity for actinobacterial supernatants and actinobacterial ethyl acetate extracts using the DPPH method. The results showed that from X. granatum, 15 isolates of endophytic actinobacteria were obtained from fruit, seeds, and leaves. Endophytic actinobacteria isolates from X. granatum showed different colors of aerial mycelium, substrate mycelium, and spore chain type. XGF11, XGF12, and XGF4 isolates produced high antioxidant activity of the supernatant; 57.30%, 49.77%, and 41.90% inhibition, respectively. The antioxidant capacity of XGF12 ethyl acetate extract was 501.60 μg AEAC/mg extract and 465.47 μg AEAC/mg extract for XGF11 ethyl acetate extract. Based on 16S rRNA gene analysis, isolates XGF12 and XGF11 showed similarities with Streptomyces xylanilyticus, and isolated XGF4 showed similarities with Brevibacterium sediminis.","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"30 6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83001558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-30DOI: 10.21776/ub.biotropika.2022.010.01.10
M. Fathoni, L. Hakim, N. Kurniawan
In Bromo Tengger Semeru National Park (BTSNP), buffer zone areas, one of which is the Lesti upstream rivers, play a critical role in sustaining biodiversity and community life. Frogs serve as a bioindicator of environmental change, particularly in the upstream rivers of the Lesti. The present study examines the diversity and community structure of Anura in the upstream rivers of Lesti, which are included in the BTSNP buffer zones. Throughout the surveys, up to 14 anuran species were encountered in four sites adjacent to the BTSNP border and crossed by the Lesti rivers. This study suggests that buffer zones may support herpetofaunal diversity by allowing humans to interact with them and sustainably utilize the resources in the areas. Urban development and conservation in these areas must be kept current in order to minimize environmental disruption caused by humans
{"title":"Anuran diversity and community structure in Lesti upriver across buffer zone habitat in Bromo Tengger Semeru National Park","authors":"M. Fathoni, L. Hakim, N. Kurniawan","doi":"10.21776/ub.biotropika.2022.010.01.10","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2022.010.01.10","url":null,"abstract":"In Bromo Tengger Semeru National Park (BTSNP), buffer zone areas, one of which is the Lesti upstream rivers, play a critical role in sustaining biodiversity and community life. Frogs serve as a bioindicator of environmental change, particularly in the upstream rivers of the Lesti. The present study examines the diversity and community structure of Anura in the upstream rivers of Lesti, which are included in the BTSNP buffer zones. Throughout the surveys, up to 14 anuran species were encountered in four sites adjacent to the BTSNP border and crossed by the Lesti rivers. This study suggests that buffer zones may support herpetofaunal diversity by allowing humans to interact with them and sustainably utilize the resources in the areas. Urban development and conservation in these areas must be kept current in order to minimize environmental disruption caused by humans","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73565703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-30DOI: 10.21776/ub.biotropika.2022.010.01.05
Andrio A Wibowo, A. Basukriadi, Erwin Nurdin
{"title":"Fire Hotspot and Particulate Matter (Pm2.5) Distribution Patterns In Tropical Rainforest Ecosystems Of Batanghari River Basin In July 2021","authors":"Andrio A Wibowo, A. Basukriadi, Erwin Nurdin","doi":"10.21776/ub.biotropika.2022.010.01.05","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2022.010.01.05","url":null,"abstract":"","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73783681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aims of this study were to isolate Lysinibacillus sphaericus from sewage at some housing complex in Mataram city and evaluate its toxicity against third Aedes aegypti larvae. The bacteria collected from mud taken from diluted sewage and objected to heat shock procedure at 80oC for 30 minutes. The microbiological culture was done using a Nutrient Agar solid medium and incubated at 30oC for 72 hours. Bacterial characterization was done based on bacterial colony morphology, cell morphology, cell physiology, and cell biochemistry characteristics. Toxicity test on 3rd Aedes aegypti larvae was done for 24, 48, and 72 hours applying Nutrient Broth medium with various dilution. From this study total of 11 isolates of Lysinibacillus sphaericus were isolated, only three isolates showed a high killing rate against Ae. aegypti in 24-hour observation. They were isolate Bs9-2-3 (LC50 24-hour value was 1.75 x 10 cell/mL), isolate Bs9-1-5 (LC50 24-hour value was 6.23 x 10 cell/mL) and isolate Bs2-1-2 (LC50 24-hour value was 7.17 x 10 cell/mL). These local isolates of L. sphaericus had good potential to be developed for bacterial-based biopesticide/biolarvicide for battling Aedes mosquito larvae in the near future.
{"title":"Isolation of Entomopathogenic Lysinibacillus sphaericus from Sewage at Some Housing Complex in Mataram City and Evaluation of Its Toxicity Against Aedes aegypti Larvae in Laboratory","authors":"Novia Devi Kusuma Dewi, E. Hidayati, Sarkono Sarkono, Eka Sunarwidhi Prasedya, Bambang Fajar Suryadi","doi":"10.21776/ub.biotropika.2022.010.01.07","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2022.010.01.07","url":null,"abstract":"The aims of this study were to isolate Lysinibacillus sphaericus from sewage at some housing complex in Mataram city and evaluate its toxicity against third Aedes aegypti larvae. The bacteria collected from mud taken from diluted sewage and objected to heat shock procedure at 80oC for 30 minutes. The microbiological culture was done using a Nutrient Agar solid medium and incubated at 30oC for 72 hours. Bacterial characterization was done based on bacterial colony morphology, cell morphology, cell physiology, and cell biochemistry characteristics. Toxicity test on 3rd Aedes aegypti larvae was done for 24, 48, and 72 hours applying Nutrient Broth medium with various dilution. From this study total of 11 isolates of Lysinibacillus sphaericus were isolated, only three isolates showed a high killing rate against Ae. aegypti in 24-hour observation. They were isolate Bs9-2-3 (LC50 24-hour value was 1.75 x 10 cell/mL), isolate Bs9-1-5 (LC50 24-hour value was 6.23 x 10 cell/mL) and isolate Bs2-1-2 (LC50 24-hour value was 7.17 x 10 cell/mL). These local isolates of L. sphaericus had good potential to be developed for bacterial-based biopesticide/biolarvicide for battling Aedes mosquito larvae in the near future.","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84244698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Drought is a threat that affects plant growth and development. Wheat crops are very sensitive to heat and drought stress during flowering and grain development, which negatively affects grain yield and quality. Wheat with drought tolerant properties is a genetically modified plant obtained by inserting a cold shock protein gene from Escherichia coli so that it can increase the ability of wheat to adapt to drought. This literature review aims to provide information about the role of cold shock protein gene insertion from Escherichia coli in wheat plants to produce drought tolerant traits. The results of the literature review showed that the CspA gene from Escherichia coli inserted into DNA could increase gene expression by changing the nucleotides in the DNA sequence to increase G+C. Gene insertion produces recombinant DNA with specific nucleotide changes. Nucleotide changes were carried out to increase the overall G+C amino acid content without changing the existing CspA and CspB protein sequences. These changes played an important role in helping wheat adapt to drought. The leaves on the transgenic wheat showed a decrease in the rate of water loss because the stomata in these plants closed faster than in the ordinary wheat so that the transgenic wheat became more drought tolerant.
{"title":"INSERTION OF COLD SHOCK GENE FROM ESCHERICHIA COLI IN WHEAT PLANTS TO PRODUCE DROUGHT TOLERANT PROPERTIES","authors":"Takashi Reymond Rimpan, Naomi Dwany, Geraldy John Meyners, Yohana Delvia Ayustin, Wahyu Irawati","doi":"10.21776/ub.biotropika.2022.010.01.03","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2022.010.01.03","url":null,"abstract":"Drought is a threat that affects plant growth and development. Wheat crops are very sensitive to heat and drought stress during flowering and grain development, which negatively affects grain yield and quality. Wheat with drought tolerant properties is a genetically modified plant obtained by inserting a cold shock protein gene from Escherichia coli so that it can increase the ability of wheat to adapt to drought. This literature review aims to provide information about the role of cold shock protein gene insertion from Escherichia coli in wheat plants to produce drought tolerant traits. The results of the literature review showed that the CspA gene from Escherichia coli inserted into DNA could increase gene expression by changing the nucleotides in the DNA sequence to increase G+C. Gene insertion produces recombinant DNA with specific nucleotide changes. Nucleotide changes were carried out to increase the overall G+C amino acid content without changing the existing CspA and CspB protein sequences. These changes played an important role in helping wheat adapt to drought. The leaves on the transgenic wheat showed a decrease in the rate of water loss because the stomata in these plants closed faster than in the ordinary wheat so that the transgenic wheat became more drought tolerant.","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"244 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74967297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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