Pub Date : 2024-04-02DOI: 10.21776/ub.biotropika.2024.012.01.01
Nur Asni Setiani, Rafika Zahraeni, Siti Uswatun Hasanah
Endophytic bacteria are in symbiosis with plants and can produce secondary metabolites similar to those produced by their host. Kumis kucing (Orthosiphon aristatus) is a traditional medicinal plant that has been proven to have many pharmacological activities, including antiviral and antibacterial. This study aims to isolate and identify endophytic bacteria from kumis kucing leaves. Endophytic bacteria were isolated from kumis kucing leaf by spreading method on Trypticase Soy Agar (TSA) media and incubated at room temperature for 24 hours. Colonies that grew with bacterial morphology were inoculated on TSA media to obtain pure cultures. Pure cultures of isolates were identified through Gram staining, 16S rRNA gene sequencing, and bacterial growth curves. There were 8 isolates with bacterial morphology which identified by Gram staining. The staining results showed that all were classified as Gram negative with rod and coccus shapes. Sequencing using the 16S rRNA gene identified 3 bacterial isolates, Acinetobacter schindleri, Pantoea agglomerans, and Pseudomonas lurida. The three bacteria have different time to reach stationary phases in order to produce their secondary metabolites.
{"title":"Isolation and Identification of Endophytic Bacteria from Kumis Kucing Leaves (Orthosiphon aristatus Benth.)","authors":"Nur Asni Setiani, Rafika Zahraeni, Siti Uswatun Hasanah","doi":"10.21776/ub.biotropika.2024.012.01.01","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2024.012.01.01","url":null,"abstract":"Endophytic bacteria are in symbiosis with plants and can produce secondary metabolites similar to those produced by their host. Kumis kucing (Orthosiphon aristatus) is a traditional medicinal plant that has been proven to have many pharmacological activities, including antiviral and antibacterial. This study aims to isolate and identify endophytic bacteria from kumis kucing leaves. Endophytic bacteria were isolated from kumis kucing leaf by spreading method on Trypticase Soy Agar (TSA) media and incubated at room temperature for 24 hours. Colonies that grew with bacterial morphology were inoculated on TSA media to obtain pure cultures. Pure cultures of isolates were identified through Gram staining, 16S rRNA gene sequencing, and bacterial growth curves. There were 8 isolates with bacterial morphology which identified by Gram staining. The staining results showed that all were classified as Gram negative with rod and coccus shapes. Sequencing using the 16S rRNA gene identified 3 bacterial isolates, Acinetobacter schindleri, Pantoea agglomerans, and Pseudomonas lurida. The three bacteria have different time to reach stationary phases in order to produce their secondary metabolites.","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140751117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.21776/ub.biotropika.2024.012.01.03
Nur Aini, S. Suharjono
Shallot base rot disease (Allium cepa L.) caused by the fungus Fusarium causes many crop losses, thus control efforts are required. Microbial biopesticides are used as an alternative to control pests and plant pathogens that are environmentally friendly and target-specific. This study aims to evaluate the diversity of liquid biopesticide bacteria and their potential to control the Fusarium sp. pathogen of shallots. This study includes shallot sampling, isolation and purification of liquid biopesticide bacteria, liquid biopesticide antifungal test, double culture antagonistic test of bacterial isolates against Fusarium sp., and identification of potential bacterial isolates based on 16S rDNA sequence similarity. The results showed that 2 bacterial isolates B7 and B2 had the highest Importance Value Index of 94.21% and 90.59% respectively, with a bacterial diversity index of 1.44. Liquid biopesticide microbial biomass has a high inhibition zone in the antifungal test against Fusarium sp. with an average inhibition zone of 6.45 mm. Bacterial isolate B2 has the highest potential inhibition of 71.3% against Fusarium sp. and was identified as Genus Serratia with a similarity value of 96.79%.
{"title":"Diversity and Potential Analysis of Liquid Biopesticide Bacteria for Fusarium sp. Control of Shallot -Basal Rot","authors":"Nur Aini, S. Suharjono","doi":"10.21776/ub.biotropika.2024.012.01.03","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2024.012.01.03","url":null,"abstract":"Shallot base rot disease (Allium cepa L.) caused by the fungus Fusarium causes many crop losses, thus control efforts are required. Microbial biopesticides are used as an alternative to control pests and plant pathogens that are environmentally friendly and target-specific. This study aims to evaluate the diversity of liquid biopesticide bacteria and their potential to control the Fusarium sp. pathogen of shallots. This study includes shallot sampling, isolation and purification of liquid biopesticide bacteria, liquid biopesticide antifungal test, double culture antagonistic test of bacterial isolates against Fusarium sp., and identification of potential bacterial isolates based on 16S rDNA sequence similarity. The results showed that 2 bacterial isolates B7 and B2 had the highest Importance Value Index of 94.21% and 90.59% respectively, with a bacterial diversity index of 1.44. Liquid biopesticide microbial biomass has a high inhibition zone in the antifungal test against Fusarium sp. with an average inhibition zone of 6.45 mm. Bacterial isolate B2 has the highest potential inhibition of 71.3% against Fusarium sp. and was identified as Genus Serratia with a similarity value of 96.79%.","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140753934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.21776/ub.biotropika.2024.012.01.02
Hana Safitri, Abdul Malik, Arnia Sari Mukaromah
Keji plant (Staurogyne elongata (Blume) Kuntze) is an herbal medicinal plant that is often found on the islands of Java to Sumatra. The leaves and roots of keji plants (S. elongata) are used by the community as diuretic drugs and increase blood pressure and are believed to treat bladder stones, kidney stones, and joint problems. However, research on secondary metabolites in the stems and roots of keji plants has never been done. The aims of the study were to identify the secondary metabolites content and marker compounds in the stems and roots of keji plants. The research stages were drying and pollination of samples, extraction, and analysis using Gas Chromatography-Mass Spectrometry (GC-MS). The results showed that the compounds in the stem were nine secondary metabolite compounds from the phenol, terpenoid, alcohol and ester group. Meanwhile, secondary metabolite compounds in the roots of keji plants were ten compounds from coumaran, phenols, and ester group. The marker compounds in the stem were phenol, 2,2'-methylenebis[6-(1,1-dimethylethyl)-4-methyl- while the marker compounds in the roots were octadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester.
{"title":"SECONDARY METABOLITE PROFILE IN STEM AND ROOT OF KEJI PLANT (Staurogyne elongata (Blume) Kuntze)","authors":"Hana Safitri, Abdul Malik, Arnia Sari Mukaromah","doi":"10.21776/ub.biotropika.2024.012.01.02","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2024.012.01.02","url":null,"abstract":"Keji plant (Staurogyne elongata (Blume) Kuntze) is an herbal medicinal plant that is often found on the islands of Java to Sumatra. The leaves and roots of keji plants (S. elongata) are used by the community as diuretic drugs and increase blood pressure and are believed to treat bladder stones, kidney stones, and joint problems. However, research on secondary metabolites in the stems and roots of keji plants has never been done. The aims of the study were to identify the secondary metabolites content and marker compounds in the stems and roots of keji plants. The research stages were drying and pollination of samples, extraction, and analysis using Gas Chromatography-Mass Spectrometry (GC-MS). The results showed that the compounds in the stem were nine secondary metabolite compounds from the phenol, terpenoid, alcohol and ester group. Meanwhile, secondary metabolite compounds in the roots of keji plants were ten compounds from coumaran, phenols, and ester group. The marker compounds in the stem were phenol, 2,2'-methylenebis[6-(1,1-dimethylethyl)-4-methyl- while the marker compounds in the roots were octadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester.","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140753323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-23DOI: 10.21776/ub.biotropika.2023.011.03.03
Ichda Arini Dinana, Widodo Widodo, Nia Kurniawan
Treatment options for diabetes may vary widely, however, around 50% of all diabetic patients do not reach the minimum glycemic target. Therefore, drugs that are able to lower glucose levels through unconventional targets are still needed for the treatment of type 2 diabetes. This study aimed to investigate the activity of cardiotoxins from N. sputatrix towards Kv channels as potential targets for glucose level reduction. Molecular docking analysis was conducted using the ClusPro web server, and the resulting protein-ligand interactions were visualized using BioVia Discovery Studio and LigPlus v.4.5.3. The stability of the docking structures was further examined through molecular dynamics simulations. The CTX-1, CTX-3, CTX-4, CTX-5, and CTX-KJC3 sequences from N. sputatrix were aligned and modeled, focusing on the 62-81 amino acid residues. The models of CTX-3, CTX-4, and CTX-5 demonstrated interactions with the binding site of the KcsA receptor. Additionally, substituting the Val74 residue in the CTX62-81 fragment with Lys74 resulted in reduced binding energy and mitigated the cytotoxic effects of CTX, while maintaining its insulinotropic activity
{"title":"Computational Design of a Truncated Javan Spitting Cobra (Naja sputatrix) Venom Cardiotoxin Analogue as a Promising Insulinotropic Agent","authors":"Ichda Arini Dinana, Widodo Widodo, Nia Kurniawan","doi":"10.21776/ub.biotropika.2023.011.03.03","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2023.011.03.03","url":null,"abstract":"Treatment options for diabetes may vary widely, however, around 50% of all diabetic patients do not reach the minimum glycemic target. Therefore, drugs that are able to lower glucose levels through unconventional targets are still needed for the treatment of type 2 diabetes. This study aimed to investigate the activity of cardiotoxins from N. sputatrix towards Kv channels as potential targets for glucose level reduction. Molecular docking analysis was conducted using the ClusPro web server, and the resulting protein-ligand interactions were visualized using BioVia Discovery Studio and LigPlus v.4.5.3. The stability of the docking structures was further examined through molecular dynamics simulations. The CTX-1, CTX-3, CTX-4, CTX-5, and CTX-KJC3 sequences from N. sputatrix were aligned and modeled, focusing on the 62-81 amino acid residues. The models of CTX-3, CTX-4, and CTX-5 demonstrated interactions with the binding site of the KcsA receptor. Additionally, substituting the Val74 residue in the CTX62-81 fragment with Lys74 resulted in reduced binding energy and mitigated the cytotoxic effects of CTX, while maintaining its insulinotropic activity","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139604716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-23DOI: 10.21776/ub.biotropika.2023.011.03.02
Wirentyas Nafika, A. Munawarti
Rootstock apple is a type of plant used in grafting propagation. Research related to the response of morphogenesis in this plant has not been widely carried out. The aim of this research was to study the effect of auxin and cytokinin ratio on the morphogenesis response of leaf and petioles explants of rootstock apple and to study variations in the ratio of auxin and cytokinin with the best response. The study used a 2-factorial Completely Randomized Design (CRD) with variations in the types of explants and combinations of plants growth regulators (PGR) in the culture medium. There were 12 treatments with five repetitions. Explants in the form of young apple leaf (third leaf) and petiole rootstock that had been sterilized and cut with a size of 5x5 mm for the leaf and 5 mm long for the petiole were inoculated into MS medium with cytokinin in the form of BAP (1 ppm) combined with auxin, IBA (0, 0.1, 0.2, 0.3, 0.4, 0.5 ppm). Each culture bottle contains five explants. The results showed that the average leaf explant was capable of producing 34% callus. Meanwhile, petiole explants produced 4% callus. Callus began to form on leaf and petiole explants on the 26th and 42nd days after inoculation respectively. Most calluses have a compact texture with variations in green, yellowish white, and brownish green. The PGR combination with the highest percentage of callus formation in leaf explants was found in the BAP 1 ppm + IBA 0.4 ppm treatment, while the petiole was in the BAP 1 ppm + IBA 0.3 ppm treatment
{"title":"Morphogenesis Response of Leaf and Petiole Explant of Rootstock Apple (Malus sylvestris Mill.) to Auxin and Cytokinin","authors":"Wirentyas Nafika, A. Munawarti","doi":"10.21776/ub.biotropika.2023.011.03.02","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2023.011.03.02","url":null,"abstract":"Rootstock apple is a type of plant used in grafting propagation. Research related to the response of morphogenesis in this plant has not been widely carried out. The aim of this research was to study the effect of auxin and cytokinin ratio on the morphogenesis response of leaf and petioles explants of rootstock apple and to study variations in the ratio of auxin and cytokinin with the best response. The study used a 2-factorial Completely Randomized Design (CRD) with variations in the types of explants and combinations of plants growth regulators (PGR) in the culture medium. There were 12 treatments with five repetitions. Explants in the form of young apple leaf (third leaf) and petiole rootstock that had been sterilized and cut with a size of 5x5 mm for the leaf and 5 mm long for the petiole were inoculated into MS medium with cytokinin in the form of BAP (1 ppm) combined with auxin, IBA (0, 0.1, 0.2, 0.3, 0.4, 0.5 ppm). Each culture bottle contains five explants. The results showed that the average leaf explant was capable of producing 34% callus. Meanwhile, petiole explants produced 4% callus. Callus began to form on leaf and petiole explants on the 26th and 42nd days after inoculation respectively. Most calluses have a compact texture with variations in green, yellowish white, and brownish green. The PGR combination with the highest percentage of callus formation in leaf explants was found in the BAP 1 ppm + IBA 0.4 ppm treatment, while the petiole was in the BAP 1 ppm + IBA 0.3 ppm treatment","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139604032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-23DOI: 10.21776/ub.biotropika.2023.011.03.04
Fikriya Novita Sari, Rizky Senna Samoedra, Setyaki Kevin Pratama, Sri Rahayu, A. Soewondo, Muhaimin Rifa'i
Diabetes mellitus is a metabolic disorder characterized by high blood glucose (hyperglycemia). Hyperglycemia will cause the body to undergo physiological changes such as free radical, antioxidant and inflammation alteration. This research aims to compare the profile of free radicals, endogenous antioxidants, and cytokines in mouse models of type 1 diabetes mellitus (T1DM). Mice were separated into two different groups, normal and diabetic mice groups. The normal group was a group of mice that were not induced to have diabetic conditions, while the diabetic mice group was induced to be diabetic using streptozotocin injection. Blood glucose levels were checked every three days for 14 days, while the immune response was evaluated after 14 days using flow cytometry. Data analysis was done using SPSS software with t-test analysis. This research showed that the increasing ROS represented by MDA would trigger inflammation in T1DM represented by the increasing TNF-alpha along with IFN-gamma and reducing anti-inflammatory cytokines represented by IL-10. Interestingly, SOD expression, which is an endogenous antioxidant, is also increased in the diabetic mice group, and we conclude that it is some sort of adaptive response of the diabetic mice group against the increasing ROS.
{"title":"A Comparative Profile of Free Radicals, Endogenous Antioxidants, and Cytokines in Mouse Model of Type 1 Diabetes Mellitus","authors":"Fikriya Novita Sari, Rizky Senna Samoedra, Setyaki Kevin Pratama, Sri Rahayu, A. Soewondo, Muhaimin Rifa'i","doi":"10.21776/ub.biotropika.2023.011.03.04","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2023.011.03.04","url":null,"abstract":"Diabetes mellitus is a metabolic disorder characterized by high blood glucose (hyperglycemia). Hyperglycemia will cause the body to undergo physiological changes such as free radical, antioxidant and inflammation alteration. This research aims to compare the profile of free radicals, endogenous antioxidants, and cytokines in mouse models of type 1 diabetes mellitus (T1DM). Mice were separated into two different groups, normal and diabetic mice groups. The normal group was a group of mice that were not induced to have diabetic conditions, while the diabetic mice group was induced to be diabetic using streptozotocin injection. Blood glucose levels were checked every three days for 14 days, while the immune response was evaluated after 14 days using flow cytometry. Data analysis was done using SPSS software with t-test analysis. This research showed that the increasing ROS represented by MDA would trigger inflammation in T1DM represented by the increasing TNF-alpha along with IFN-gamma and reducing anti-inflammatory cytokines represented by IL-10. Interestingly, SOD expression, which is an endogenous antioxidant, is also increased in the diabetic mice group, and we conclude that it is some sort of adaptive response of the diabetic mice group against the increasing ROS.","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139604466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-23DOI: 10.21776/ub.biotropika.2023.011.03.01
Thomas Frans Pattiasina, Ihwan Tjolli, Aplena Ellen Bless, Krisma Lekitoo
Data and information on resource utilization supported by vegetation mapping is the basis for developing a sustainable mangrove management strategy. This study aims to map the density of vegetation and identify the use of mangroves by the community in the Klawalu Mangrove Tourism Park (Taman Wisata Mangrove Klawalu-TWMK) Sorong City. The satellite imagery used is Sentinel 2A level 1C. Image pre-processing includes atmospheric correction, geometric correction and image cropping. Determination of the extent and distribution of mangrove vegetation is carried out by applying the color composite method and mangrove boundary delineation. Mangrove vegetation density mapping was carried out by applying the Normalized Difference Vegetation Index (NDVI) method. Identification of the species and intensity of mangrove utilization was carried out through observation and interviews with respondents who were determined purposively. Based on the results of the analysis, the total area of mangrove vegetation in TWMK and its surroundings which is mapped is 144.64 ha. Of this area, 69.01 ha (47.71%) is classified as very dense, 34.96 ha (24.17%) is classified as dense and 24.23 ha (16.75%) is classified as moderate, 12. 94 ha (8.95%) is classified as sparse, and 3.5 ha (2.42%) is classified as very sparse. The intensity of resource utilization from the mangrove ecosystem is quite high, especially the utilization of mangrove wood and coral mining. Awareness/education efforts need to be continued, as well as alternative livelihood solutions to reduce the heavy dependence on mangrove resources.
{"title":"A Mangrove Density Mapping for Sustainable Utilization and Management in Klawalu Mangrove Tourism Park, Sorong City","authors":"Thomas Frans Pattiasina, Ihwan Tjolli, Aplena Ellen Bless, Krisma Lekitoo","doi":"10.21776/ub.biotropika.2023.011.03.01","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2023.011.03.01","url":null,"abstract":"Data and information on resource utilization supported by vegetation mapping is the basis for developing a sustainable mangrove management strategy. This study aims to map the density of vegetation and identify the use of mangroves by the community in the Klawalu Mangrove Tourism Park (Taman Wisata Mangrove Klawalu-TWMK) Sorong City. The satellite imagery used is Sentinel 2A level 1C. Image pre-processing includes atmospheric correction, geometric correction and image cropping. Determination of the extent and distribution of mangrove vegetation is carried out by applying the color composite method and mangrove boundary delineation. Mangrove vegetation density mapping was carried out by applying the Normalized Difference Vegetation Index (NDVI) method. Identification of the species and intensity of mangrove utilization was carried out through observation and interviews with respondents who were determined purposively. Based on the results of the analysis, the total area of mangrove vegetation in TWMK and its surroundings which is mapped is 144.64 ha. Of this area, 69.01 ha (47.71%) is classified as very dense, 34.96 ha (24.17%) is classified as dense and 24.23 ha (16.75%) is classified as moderate, 12. 94 ha (8.95%) is classified as sparse, and 3.5 ha (2.42%) is classified as very sparse. The intensity of resource utilization from the mangrove ecosystem is quite high, especially the utilization of mangrove wood and coral mining. Awareness/education efforts need to be continued, as well as alternative livelihood solutions to reduce the heavy dependence on mangrove resources.","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139604870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-01DOI: 10.21776/ub.biotropika.2023.011.02.05
Septiawan Putranto, Sri Rahayu, G. Ciptadi, Siska Nanda Widhaningrum
Water clover (Marsilea crenata) and moringa leaves (Moringa oleifera) are types of herbal plants that are widely used in various alternative medicines. Pharmacologically, both have active compounds such as flavonoids which have cellular activities such as antioxidants and estrogenic. This study aims to evaluate the effect of a combination of water clover ethanol extract and Moringa leaf ethanol extract with different treatment groups with the same concentration on follicle and oocyte growth based on follicle diameter and oocyte diameter after six days of culture. This experimental study used 24 antral follicles (2.5-3.2 mm) which were isolated, and randomly divided into four treatment groups namely K (control) without supplementation of water clover ethanol extract and moringa leaf ethanol extract, group D1 with water clover ethanol extract, group D2 with supplementation of moringa leaf ethanol extract, and group D3 with supplementation of a combination of water clover ethanol extract and moringa leaf
{"title":"Effect of a Combination of Water Clover (Marsilea Crenata) Ethanol Extract and Moringa Leaf (Moringa oleifera) Ethanol Extract on Follicle and Oocyte Diameter of Goat: In Vitro Study","authors":"Septiawan Putranto, Sri Rahayu, G. Ciptadi, Siska Nanda Widhaningrum","doi":"10.21776/ub.biotropika.2023.011.02.05","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2023.011.02.05","url":null,"abstract":"Water clover (Marsilea crenata) and moringa leaves (Moringa oleifera) are types of herbal plants that are widely used in various alternative medicines. Pharmacologically, both have active compounds such as flavonoids which have cellular activities such as antioxidants and estrogenic. This study aims to evaluate the effect of a combination of water clover ethanol extract and Moringa leaf ethanol extract with different treatment groups with the same concentration on follicle and oocyte growth based on follicle diameter and oocyte diameter after six days of culture. This experimental study used 24 antral follicles (2.5-3.2 mm) which were isolated, and randomly divided into four treatment groups namely K (control) without supplementation of water clover ethanol extract and moringa leaf ethanol extract, group D1 with water clover ethanol extract, group D2 with supplementation of moringa leaf ethanol extract, and group D3 with supplementation of a combination of water clover ethanol extract and moringa leaf","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85490056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-01DOI: 10.21776/ub.biotropika.2023.011.02.01
Kartika Prabasari, Nia Kurnianingsih, N. Kurniawan
The antivenom for Malayan krait (Bungarus candidus) venom has not yet been available in Indonesia, leading to many fatal snakebite cases. Alternative treatment approaches using medicinal plants are needed to be explored. This study investigated the potential of medicinal plants natural bioactive compounds as toxic alpha-delta protein bungarotoxin inhibitor in B. candidus venom. The approach taken is using the 3D structure of the alpha-delta protein of bungarotoxin B. candidus predicted by SWISS-MODEL. Knapsack Family Database and PubChem were used for bioactive compounds datamining.
马来亚金甲蛇毒的抗蛇毒血清在印度尼西亚还没有得到，导致许多致命的蛇咬伤病例。需要探索利用药用植物的替代治疗方法。本研究探讨了药用植物天然活性化合物作为毒毒素α - δ蛋白抑制剂的潜力。采用的方法是利用SWISS-MODEL预测的班加罗毒素念珠菌α - δ蛋白的三维结构。使用backpack Family Database和PubChem进行生物活性化合物的数据挖掘。
{"title":"In silico Exploration of Bioactive Compounds from Withania somnifera as Inhibitor for Alpha Delta Bungarotoxin of Bungarus candidus Venom","authors":"Kartika Prabasari, Nia Kurnianingsih, N. Kurniawan","doi":"10.21776/ub.biotropika.2023.011.02.01","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2023.011.02.01","url":null,"abstract":"The antivenom for Malayan krait (Bungarus candidus) venom has not yet been available in Indonesia, leading to many fatal snakebite cases. Alternative treatment approaches using medicinal plants are needed to be explored. This study investigated the potential of medicinal plants natural bioactive compounds as toxic alpha-delta protein bungarotoxin inhibitor in B. candidus venom. The approach taken is using the 3D structure of the alpha-delta protein of bungarotoxin B. candidus predicted by SWISS-MODEL. Knapsack Family Database and PubChem were used for bioactive compounds datamining.","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91086776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asteroidea is a marine invertebrate that has a role as a bioindicator, but the research related to abundance and potential of Asteroidea as a bioindicator of lead on Madura Island is not to be done. This study aimed to analyze the effect of Pb concentration on Asteroidea population density in Bangkalan Regency. The transect plotting method with a plot measuring 1x1 m2 with as 30 plots in the intertidal zone for collecting data. Seawater sampling was divided into three stations, such as station I-0 meters (edge), station II-50 meters (middle) and station III-100 meters (deep zone). Data analysis was carried out with quantitative descriptive with Asteroida composition including diversity index, relative density and dominance index as parameters. The results obtained were species of starfish, Cryptasterina sp. and Aquilonastra coronata with a diversity index value of 0, a relative density of 100% and a dominance index of 1. The results for Pb concentration on Tengket Beach at a depth of 0 meters, 50 meters and 100 are 0.072 ppm; 0.16 ppm; 0.61 ppm and Pantai Modung at 0.5 ppm; 0.83 ppm; 0.82 ppm. Based on the analysis of the abundance of Asteroidea, as higher of Pb concentration contained in the waters, the lower Asteroidea’s density will be found. It concluded the abundance of Asteroidea population can be used as an indicator of Pb in coastal waters.
{"title":"Abundance and Potential of Asteroidea as Bioindicator of Lead (Pb) in the Coastal Zone at Bangkalan Regency, Madura","authors":"Yusfita Kurniawati, Arnetta Yolanda Maramis, Istatik Mudloifah, Dwi Anggorowati Rahayu","doi":"10.21776/ub.biotropika.2023.011.02.04","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2023.011.02.04","url":null,"abstract":"Asteroidea is a marine invertebrate that has a role as a bioindicator, but the research related to abundance and potential of Asteroidea as a bioindicator of lead on Madura Island is not to be done. This study aimed to analyze the effect of Pb concentration on Asteroidea population density in Bangkalan Regency. The transect plotting method with a plot measuring 1x1 m2 with as 30 plots in the intertidal zone for collecting data. Seawater sampling was divided into three stations, such as station I-0 meters (edge), station II-50 meters (middle) and station III-100 meters (deep zone). Data analysis was carried out with quantitative descriptive with Asteroida composition including diversity index, relative density and dominance index as parameters. The results obtained were species of starfish, Cryptasterina sp. and Aquilonastra coronata with a diversity index value of 0, a relative density of 100% and a dominance index of 1. The results for Pb concentration on Tengket Beach at a depth of 0 meters, 50 meters and 100 are 0.072 ppm; 0.16 ppm; 0.61 ppm and Pantai Modung at 0.5 ppm; 0.83 ppm; 0.82 ppm. Based on the analysis of the abundance of Asteroidea, as higher of Pb concentration contained in the waters, the lower Asteroidea’s density will be found. It concluded the abundance of Asteroidea population can be used as an indicator of Pb in coastal waters.","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73376661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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