Pub Date : 2022-03-30DOI: 10.21776/ub.biotropika.2022.010.01.09
Idris Hermawan, M. Amin, Suhadi Suhadi
The springtails belong to the Collembola subclass of insect class and are a mesofauna insect that acts as a detritivore and bio-indicator in the ecosystem. Genetic diversity is influenced by the total genetic variation present in genes coding for species or organisms. COI gene is a gene that is useful as a genetic marker for mitochondrial DNA. The genetic diversity of springtails based on mtDNA COI gene sequences is not widely known in Malang. Based on this, the study aims to reveal the genetic diversity of springtails in Malang. This research was conducted by three locations in Malang (Cangar, Bedengan and Tambakasri). Measurements of microclimate (humidity and air temperature) and altitude measurements were carried out at springtails sampling, then carried out DNA isolation process from springtails samples obtained, COI amplification process and sequencing were carried out. After obtaining the sequence data, genetic diversity and polymorphism analysis were carried out. Results of this study were the target genes amplified from all samples of springtails with DNA fragments along 677-683 bp. The sample of springtails had a genetic identity match with Homidia socia Denis and Homidia cingula Börner based on the BLAST analysis, results of genetic diversity analysis showed that samples tested had high genetic diversity, the composition of nucleotide bases A/T was 60,8%, genetic variation in the form of transition substitution (87 sites) and transversion (55 sites), and the results of polymorphism analysis showed the value of 0,18201 nucleotide diversity, number of segregation was 162 sites and sequences conservation was 0,744.
{"title":"Genetic diversity of Springtails (Collembola Subclass) Based on Cytochrome oxidase Subunit I (COI) Genes in Malang","authors":"Idris Hermawan, M. Amin, Suhadi Suhadi","doi":"10.21776/ub.biotropika.2022.010.01.09","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2022.010.01.09","url":null,"abstract":"The springtails belong to the Collembola subclass of insect class and are a mesofauna insect that acts as a detritivore and bio-indicator in the ecosystem. Genetic diversity is influenced by the total genetic variation present in genes coding for species or organisms. COI gene is a gene that is useful as a genetic marker for mitochondrial DNA. The genetic diversity of springtails based on mtDNA COI gene sequences is not widely known in Malang. Based on this, the study aims to reveal the genetic diversity of springtails in Malang. This research was conducted by three locations in Malang (Cangar, Bedengan and Tambakasri). Measurements of microclimate (humidity and air temperature) and altitude measurements were carried out at springtails sampling, then carried out DNA isolation process from springtails samples obtained, COI amplification process and sequencing were carried out. After obtaining the sequence data, genetic diversity and polymorphism analysis were carried out. Results of this study were the target genes amplified from all samples of springtails with DNA fragments along 677-683 bp. The sample of springtails had a genetic identity match with Homidia socia Denis and Homidia cingula Börner based on the BLAST analysis, results of genetic diversity analysis showed that samples tested had high genetic diversity, the composition of nucleotide bases A/T was 60,8%, genetic variation in the form of transition substitution (87 sites) and transversion (55 sites), and the results of polymorphism analysis showed the value of 0,18201 nucleotide diversity, number of segregation was 162 sites and sequences conservation was 0,744.","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86027112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-30DOI: 10.21776/ub.biotropika.2022.010.01.02
M. Ihsan, R. Azrianingsih, R. Rugayah, Muhammad Efendi
{"title":"Morphological Variations Species of Ziziphus (Rhamnaceae) in Java","authors":"M. Ihsan, R. Azrianingsih, R. Rugayah, Muhammad Efendi","doi":"10.21776/ub.biotropika.2022.010.01.02","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2022.010.01.02","url":null,"abstract":"","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"183 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76885983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-30DOI: 10.21776/ub.biotropika.2022.010.01.04
Andista Firstiantono, Sri Rahayu, Agung Pramana Warih Marhendra, A. Soewondo
{"title":"Potential of Combination Marsilea crenata and Curcuma xanthorriza to Improve Sperm Quality of Male Mice Exposed by Monosodium Glutamate","authors":"Andista Firstiantono, Sri Rahayu, Agung Pramana Warih Marhendra, A. Soewondo","doi":"10.21776/ub.biotropika.2022.010.01.04","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2022.010.01.04","url":null,"abstract":"","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"15 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72638294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-30DOI: 10.21776/ub.biotropika.2022.010.01.06
Romario Dion, Muhammad Farrel Ewaldo, Muhammad Faishal Fauzaan, Ilham Aris Wandi, Rina Sari Asih
{"title":"In silico Exploration of Phospholipase A2 Inhibitor Compounds from Lufariella variabilis as Antivenom of Ophiophagus hannah","authors":"Romario Dion, Muhammad Farrel Ewaldo, Muhammad Faishal Fauzaan, Ilham Aris Wandi, Rina Sari Asih","doi":"10.21776/ub.biotropika.2022.010.01.06","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2022.010.01.06","url":null,"abstract":"","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84101381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-30DOI: 10.21776/ub.biotropika.2022.010.01.08
Rudy Hermawan, Tryanisa Ridla Amalia
{"title":"Ceratiomyxa arbuscula: A White Tubular Myxomycetes in West Java","authors":"Rudy Hermawan, Tryanisa Ridla Amalia","doi":"10.21776/ub.biotropika.2022.010.01.08","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2022.010.01.08","url":null,"abstract":"","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84681812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21776/ub.biotropika.2022.010.02.01
Agung Sih Kurnianto, Santi Prastiwi, N. Dewi, Ahmad Ilham Tanzil, Wildan Muhlison, Wagiyana Wagiyana
Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae) is the biggest threat to onion farming, especially in the vegetative phase. In severe attacks, this pest can cause yield losses of up to 100%. Compatibility is one way to increase the efficiency and effectiveness of pest control by using plant-based pesticides or biological agents. Neem Leaf Extract (Azadirachta indica, acronym=NLE) has an azadirachtin compound that can inhibit insect growth, reduce appetite, reproduction, and hatch eggs. The fungus Beauveria bassiana (acronym=Bb) can secrete chitinase, lipase, proteinase enzymes that are able to decompose insect cuticles. NLE was obtained through the extraction method of plant-based pesticides and stored at 4oC until the experiment time. The Bb used was a commercial B. bassiana inoculum in the flour form with a density of 4.5 x 1010 spores/gram (trade name = Natural BVR). To determine the advantages of compatibility, this study is held by a single toxicity test of Bb and NLE, and the combined toxicity test of both. This study used a completely randomized design (CRD) consisting of 6 treatments with 4 replications, each replication used 12 larvae (a total of 48 larvae). Combination toxicity has a higher toxicity than the single use of NLE and Bb. At the LC 95 level, the combination treatment of Bb and NLE was 1.06-1.15 times more toxic than the single treatment of Bb, and 6.87-7.79 times more toxic than the single treatment of NLE. NLE and Bb are considered to have high compatibility (strong synergistic with GI value <0.5). Theoretically, the compatibility of NLE plant-based pesticides and Bb is very promising to replace chemical pesticides that have long-term adverse effects on agroecosystems
{"title":"Compatibility of Neem (Azadirachta indica) and Beauveria bassiana for control of Spodoptera exigua and the Theoritical Impact to The Agroecorsystem","authors":"Agung Sih Kurnianto, Santi Prastiwi, N. Dewi, Ahmad Ilham Tanzil, Wildan Muhlison, Wagiyana Wagiyana","doi":"10.21776/ub.biotropika.2022.010.02.01","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2022.010.02.01","url":null,"abstract":"Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae) is the biggest threat to onion farming, especially in the vegetative phase. In severe attacks, this pest can cause yield losses of up to 100%. Compatibility is one way to increase the efficiency and effectiveness of pest control by using plant-based pesticides or biological agents. Neem Leaf Extract (Azadirachta indica, acronym=NLE) has an azadirachtin compound that can inhibit insect growth, reduce appetite, reproduction, and hatch eggs. The fungus Beauveria bassiana (acronym=Bb) can secrete chitinase, lipase, proteinase enzymes that are able to decompose insect cuticles. NLE was obtained through the extraction method of plant-based pesticides and stored at 4oC until the experiment time. The Bb used was a commercial B. bassiana inoculum in the flour form with a density of 4.5 x 1010 spores/gram (trade name = Natural BVR). To determine the advantages of compatibility, this study is held by a single toxicity test of Bb and NLE, and the combined toxicity test of both. This study used a completely randomized design (CRD) consisting of 6 treatments with 4 replications, each replication used 12 larvae (a total of 48 larvae). Combination toxicity has a higher toxicity than the single use of NLE and Bb. At the LC 95 level, the combination treatment of Bb and NLE was 1.06-1.15 times more toxic than the single treatment of Bb, and 6.87-7.79 times more toxic than the single treatment of NLE. NLE and Bb are considered to have high compatibility (strong synergistic with GI value <0.5). Theoretically, the compatibility of NLE plant-based pesticides and Bb is very promising to replace chemical pesticides that have long-term adverse effects on agroecosystems","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89241337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-17DOI: 10.21776/ub.biotropika.2021.009.03.01
Frenly Marvi Selano, R. Hadi Purwanto, Probo Santoso
{"title":"Plants Potential of Green and Open Space Planning (RTH) to Mitigate CO2 Gas Emission in Ambon","authors":"Frenly Marvi Selano, R. Hadi Purwanto, Probo Santoso","doi":"10.21776/ub.biotropika.2021.009.03.01","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2021.009.03.01","url":null,"abstract":"","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"108 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85657715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-17DOI: 10.21776/ub.biotropika.2021.009.03.08
Selis Meriem
{"title":"Review: Regulation of Phytohormones and Environmental Stresses to Increase the Production of Plant Secondary Metabolites","authors":"Selis Meriem","doi":"10.21776/ub.biotropika.2021.009.03.08","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2021.009.03.08","url":null,"abstract":"","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75917524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-17DOI: 10.21776/ub.biotropika.2021.009.03.07
Hamdani Dwi Prasetyo, M. Ramadhan
{"title":"Quality Profile of Riparian Zone and Vegetation Quality in Amprong River, Tumpang District Based on QBR index and NDVI","authors":"Hamdani Dwi Prasetyo, M. Ramadhan","doi":"10.21776/ub.biotropika.2021.009.03.07","DOIUrl":"https://doi.org/10.21776/ub.biotropika.2021.009.03.07","url":null,"abstract":"","PeriodicalId":9004,"journal":{"name":"Biotropika: Journal of Tropical Biology","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83097773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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