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A Comparative Evaluation and Utilization of Entrapped Bacillus sp. (Gen Bank MN 243657) and Pseudomonas aeruginosa SU-1 in Poultry Waste Degradation 包埋芽孢杆菌sp. (Gen Bank MN 243657)与铜绿假单胞菌SU-1在家禽粪便降解中的比较评价与利用
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.072072
Bacillus sp. (Genbank MN243657) was obtained from the Center for Bioscience and Nanoscience Research, and P. aeruginosa SU-1 were used in this study. Initially, biomass was optimized for the incubation period, pH, and temperature, following biomass optimization with RSM. The obtained organisms were able to produce keratinase, which was found to be a 64 KDa protein and 56KDa. Now the biomass produced using the optimized condition was immobilized and checked for its ability to degrade keratinase, and efficiency was compared. Immobilized P. aeruginosa SU-1 was degrading keratin and feather better than Bacillus sp. (Genbank MN243657). It was found to reduce featherweight by 57.3% and 41%. Hence it was found that both the immobilized bacterium can be used for poultry waste treatment, and P. aeruginosa SU-1 was more efficient than Bacillus sp. (Genbank MN243657).
Bacillus sp. (Genbank MN243657)来自生物科学与纳米科学研究中心,P. aeruginosa SU-1用于本研究。首先,在RSM优化生物量之后,对生物量进行了孵育期、pH和温度的优化。获得的生物能够产生角化酶,发现它是64 KDa蛋白和56KDa蛋白。将优化条件下产生的生物质进行固定化,考察其降解角化酶的能力,并比较其效率。固定化铜绿假单胞菌SU-1对角蛋白和羽毛的降解效果优于芽孢杆菌(Genbank MN243657)。研究发现,它可以减少57.3%和41%的羽毛重量。结果表明,这两种固定化菌均可用于处理家禽废弃物,且P. aeruginosa SU-1的处理效率高于Bacillus sp. (Genbank MN243657)。
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引用次数: 0
Azolla pinnata: potential phytoremediation, antimicrobial and antioxidant applications 小红花:潜在的植物修复、抗菌和抗氧化应用
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.081081
Azolla is an aquatic nitrogen-fixing pteridophyte with a wide distribution in temperate and tropical freshwater ecosystems and paddy fields. Its feasibility as a source for the development of health supplements was tested by analyzing the phytochemical, antimicrobial, and antioxidant properties of the fern. The plant was found to be rich in phenolic compounds like tannins, saponins, etc. which shows it will be of good medicinal value. The DPPH antioxidant activity of the various extracts shows the good presence of antioxidants, which shows its potential use as health supplements. A fair antibacterial activity was shown against the disease, causing bacteria Staphylococcus sp. and Bacillus sp. It can be concluded that the fern has good nutritional and antibacterial activity. The phytoremediation property of Azolla grown in a metal-containing sample was assessed using atomic absorption spectroscopy, and positive results indicated its prospective use in industrial or laboratory wastewater treatment. Thus Azolla is an ideal candidate for future research in food, feed, and fodder applications. It can be utilized as a natural plant-based antimicrobial and also as a water purifier.
杜鹃花是一种水生固氮蕨类植物,广泛分布于温带和热带淡水生态系统和稻田中。通过分析蕨类植物的化学、抗菌和抗氧化特性,对其作为保健品开发来源的可行性进行了测试。该植物富含单宁、皂苷等酚类化合物,具有良好的药用价值。各种提取物的DPPH抗氧化活性显示了抗氧化剂的良好存在,显示了其作为保健品的潜在用途。对病原菌葡萄球菌和芽孢杆菌均有较好的抑菌活性,具有良好的营养和抑菌活性。利用原子吸收光谱法研究了生长在含金属样品中的杜鹃花的植物修复性能,结果表明其在工业或实验室废水处理中具有良好的应用前景。因此,杜鹃花是未来研究食品、饲料和饲料应用的理想候选者。它可以用作天然植物基抗菌剂,也可以用作净水器。
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引用次数: 0
Excessive Daytime Sleepiness Among Medical Students – A Cross-Sectional Survey to Study the Risk Factors 医学生日间过度嗜睡——一项研究危险因素的横断面调查
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.034034
Sleep is a condition of the body and mind which typically occurs for several hours every night, in which the nervous system is inactive, the eyes are closed, the postural muscles are relaxed, and consciousness is practically suspended. Daytime sleepiness, or difficulty in maintaining a desired level of wakefulness, is frequently viewed by the general population as a common experience and predictable consequence of insufficient sleep. The aim of this study was to determine the prevalence of excessive daytime sleepiness and factors associated with medical students in a private university in Malaysia. A cross-sectional study was conducted among year 1 to year 5 medical students. A self-administered questionnaire was used to collect data from the respondents. The data were analyzed using the Statistical Package of Social Sciences (SPSS) version 19.0. A minimum sample size of 400 was obtained. The percentage of Excessive day time sleepiness (EDS) among the students was 44% (176 of 400). Association between EDS and other independent variables was seen by the Chi-square test with p-value < 0.05 considered as a significant association. Of the various factors determined, a statistically significant association was found between internet usage and EDS.
睡眠是身体和精神的一种状态,通常每晚持续几个小时。在这种状态下,神经系统不活动,眼睛闭着,体位肌肉放松,意识实际上处于暂停状态。白天嗜睡,或难以保持理想的清醒水平,通常被普通人群视为睡眠不足的常见经历和可预见的后果。本研究的目的是确定马来西亚一所私立大学医学生日间过度嗜睡的患病率及其相关因素。本研究对一至五年级医学生进行横断面研究。一份自我管理的问卷被用来收集调查对象的数据。使用SPSS 19.0版社会科学统计软件包对数据进行分析。最小样本量为400。400名学生中有176名学生白天过度嗜睡(EDS),占44%。经卡方检验,EDS与其他自变量之间存在相关性,p值< 0.05为显著相关。在确定的各种因素中,发现互联网使用与EDS之间存在统计学上显著的关联。
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引用次数: 0
Computing a Smart Device to Classify Recurring Outbreak of Copd 计算智能设备对慢性阻塞性肺病复发进行分类
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.033033
Health care has experienced an unprecedented rise in the last decade. In order to keep their vital signs measured, patients will have to make regular visits to the doctor. The Cough detection device helps in the diagnosis and treatment of respiratory problems at an early age. Cough aims to remove mucus and bronchial diseases, which plays an important role in prevention and cure. More than 3 million individuals died of COPD in 2012, which is equivalent to 6 percent of all fatalities worldwide a year, according to world estimates. Citizens consider it impossible to track cough-related illnesses in rural areas because of the sophistication of the system and its testing costs. Nowadays, some people do have a mix of COPD and asthma. There is an immense demand for non-invasive ways of calculating these vital signs. This paper aims to develop and deploy a safe, inexpensive, low powered, non-intrusive, and effective device that can be worn daily and track the vital signs and show the performance to the mobile phone. The physician can also conveniently access such data through a wireless medium. This paper deals primarily with the acquisition of vital signs for signal conditioning and data: ECG, heart rate, blood pressure, body temperature, Sp02, cough rate, and respiration rate. This work proposes an innovative wearable cough detection method for early diagnosis and very early treatment of pulmonary diseases.
在过去十年中,医疗保健经历了前所未有的增长。为了测量他们的生命体征,病人必须定期去看医生。咳嗽检测装置有助于早期呼吸道疾病的诊断和治疗。咳嗽的目的是清除黏液和支气管疾病,在防治中起着重要作用。据世界估计,2012年有300多万人死于慢性阻塞性肺病,相当于全球每年死亡人数的6%。由于系统的复杂性和检测成本,农村居民认为不可能追踪与咳嗽有关的疾病。现在,有些人确实同时患有慢性阻塞性肺病和哮喘。对于计算这些生命体征的非侵入性方法有着巨大的需求。本文旨在开发和部署一种安全,廉价,低功耗,非侵入性,有效的设备,可以日常佩戴,跟踪生命体征并向手机显示性能。医生也可以通过无线媒介方便地访问这些数据。本文主要研究生命体征的信号调理和数据采集:心电图、心率、血压、体温、Sp02、咳嗽率和呼吸率。本工作提出了一种创新的可穿戴咳嗽检测方法,用于肺部疾病的早期诊断和早期治疗。
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引用次数: 0
Characterization of Phycocyanin Pigment from Marine Filamentous Cyanobacteria Geitlerinema sp. 海洋丝状蓝藻Geitlerinema sp.藻蓝色素的表征
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.074074
Algae is a simple and vast group of photosynthetic organisms. Algae grow in most of the natural environments, including water, rocks, and soil, but interestingly also grow on and in other organisms. Their main habitats are freshwater, brackish and marine ecosystems, Also volcanic waters and salt waters. Among them, marine algae are recognized as a rich source of beneficial bioactive compounds. Marine algal originated compounds have been reported to exhibit various biological activities such as anticoagulant, anti-viral, antioxidant, anti-allergic, anti-cancer, anti-inflammatory, anti-obesity, etc. Phycocyanin is a blue-colored naturally existing phycobiliprotein pigment, which is abundant in cyanobacteria, blue-green algae. In this study, phycocyanin was extracted from the Geitlerinema sp. UV-Vis spectroscopic analysis and HPLC analysis confirmed the presence of phycocyanin. The extracted pigment was assessed for their antioxidant property by various methods such as Phosphomolybdenum assay, Ferric ions reducing power assay, DPPH radical scavenging activity, Hydrogen peroxide free radical scavenging assay, Anti-lipid peroxidation activity. The study reveals that the phycocyanin pigments possess the antioxidant property, and it can be used as a promising pharmaceutical and nutraceutical compound.
藻类是一种简单而庞大的光合生物。藻类生长在大多数自然环境中,包括水、岩石和土壤,但有趣的是,藻类也生长在其他生物身上或体内。它们的主要栖息地是淡水、咸淡水和海洋生态系统,也有火山水和咸水。其中,海藻被认为是有益生物活性化合物的丰富来源。据报道,海藻源化合物具有抗凝血、抗病毒、抗氧化、抗过敏、抗癌、抗炎、抗肥胖等多种生物活性。藻蓝蛋白是一种天然存在的蓝色藻胆蛋白色素,在蓝藻、蓝藻中含量丰富。本研究从Geitlerinema sp.中提取了藻蓝蛋白,紫外可见光谱分析和高效液相色谱分析证实了藻蓝蛋白的存在。采用磷钼法、铁离子还原力法、DPPH自由基清除法、过氧化氢自由基清除法、抗脂质过氧化活性等方法评价提取的色素的抗氧化性能。研究表明,藻蓝蛋白具有良好的抗氧化性能,是一种很有前途的药用和营养保健化合物。
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引用次数: 0
Fabrication of Gelatin/Chitosan/Zeolite Composite Packaging Film for Enhancing the Quality of Fresh Meat During Storage 明胶/壳聚糖/沸石复合包装膜的制备提高鲜肉贮藏品质
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.053053
R. Rajan, T. K. Gopal
Valorisation of fishery by-products for the extraction of gelatin-like biopolymers is an active area of research presently due to their significant food and biomedical applications. In the present study, gelatin was extracted from the discarded portions of the Amphioctopusmarginatus. One of the major octopus species landed near the Kerala coast. Physico-chemical and functional characterization of the extracted gelatin was done based on SDS- PAGE, UV-Vis spectrophotometer, FTIR, XRD, HPLC, gelling and emulsifying capacities. Later, biocomposite film based on the gelatin/chitosan/zeolite was developed and characterized according to its solubility, opacity, spectroscopic analysis, antioxidative and antimicrobial activities. The results showed the dimensions of improving the properties of biopolymer films in terms of its ability to prevent the oxidative lipid changes, protein degradation and microbial spoilage in packaged fresh chicken meat over 9 days of refrigerated storage. The gelatin/chitosan/zeolite composite film thus developed has the potential to serve as a promising bio-based packaging material in the food packaging and meat processing industries.
由于明胶样生物聚合物在食品和生物医学上的重要应用,渔业副产品的价值提取目前是一个活跃的研究领域。在本研究中,明胶是从边角两栖章鱼的废弃部分中提取的。其中一种主要的章鱼降落在喀拉拉邦海岸附近。通过SDS- PAGE、紫外-可见分光光度计、FTIR、XRD、HPLC、胶凝和乳化性能对提取的明胶进行了理化和功能表征。随后,开发了明胶/壳聚糖/沸石复合生物膜,并对其溶解度、不透明度、光谱分析、抗氧化和抗菌活性进行了表征。研究结果表明,生物聚合物薄膜对包装新鲜鸡肉中氧化性脂质变化、蛋白质降解和微生物腐败的抑制作用有待进一步提高。由此制备的明胶/壳聚糖/沸石复合膜在食品包装和肉类加工行业中具有广阔的应用前景。
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引用次数: 0
Mixed fruit wastes as a potential source for Bacteriocin Producing Lactic acid bacterium – Lactobacillus saniviri NKSS1 混合水果废料作为产生细菌素的乳酸菌-桑尼韦里乳杆菌NKSS1的潜在来源
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.099099
Mixed fruit juices contain microflora on the surface of fruits during the harvest and post-harvest practices. The presence of useful organisms like lactic acid bacteria from the mixed fruit wastes was explored in this study since these microbes use these wastes as a nutrient source for their growth. The lactic acid bacteria isolated using MRS medium was identified as Lactobacillus saniviri NKSS1 by 16s rRNA analysis. The bacteriocin produced by Lactobacillus saniviri NKSS1 showed inhibitory effect against the food pathogen (Listeria monocytogenes) and clinical pathogen (Acinetobacter baumannii). Optimization of bacteriocin production from Lactobacillus saniviri NKSS1 was achieved at 24 h of incubation, temperature at 35 °C with the initial medium pH of 6.5. The carbon & nitrogen sources like dextrose (3% w/v) and yeast extract (0.75% w/v) enhanced the production of bacteriocin in MRS medium. Antimicrobial activity was reduced in the partially purified bacteriocin when incubated at 95 °C for 2 h but it retained its activity in the pH range of 5.5 to 8.5. Whereas, metals like CuSO4 and MgSO4 at (0.5 % w/v) interfered with the antagonistic activity of partially purified bacteriocin. Ionic detergents like SDS and CTAB partially decreased the antimicrobial activity, while other non-ionic detergents inhibited the antimicrobial activity completely. The molecular weight of partially purified bacteriocin from Lactobacillus saniviri NKSS1 was found to be 10.9 kDa. It can be concluded that bacteriocin of L. saniviri NKSS1 holds a promising potential for extension of shelf-life and improvement of microbiological safety in food industries.
在收获和收获后的实践中,混合果汁在水果表面含有微生物群。由于这些微生物利用这些废物作为其生长的营养来源,因此本研究探索了混合水果废物中乳酸菌等有益生物的存在。MRS培养基分离得到的乳酸菌经16s rRNA分析鉴定为桑尼viri乳杆菌NKSS1。saniviri乳杆菌NKSS1产生的细菌素对食品致病菌(单核增生李斯特菌)和临床致病菌(鲍曼不动杆菌)均有抑制作用。在培养24 h,温度35℃,初始培养基pH为6.5的条件下,对桑尼viri乳杆菌NKSS1产菌素进行了优化。葡萄糖(3% w/v)和酵母浸膏(0.75% w/v)等碳氮源均能提高MRS培养基中细菌素的产量。部分纯化的细菌素在95℃下孵育2 h,抑菌活性降低,但在pH为5.5 ~ 8.5范围内仍保持抑菌活性。而金属如CuSO4和MgSO4在(0.5% w/v)下干扰部分纯化的细菌素的拮抗活性。SDS和CTAB等离子型洗涤剂部分降低了抗菌活性,而其他非离子型洗涤剂则完全抑制了抗菌活性。从saniviri乳杆菌NKSS1中部分纯化的细菌素分子量为10.9 kDa。由此可见,saniviri NKSS1细菌素在延长食品保质期和提高食品微生物安全性方面具有广阔的应用前景。
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引用次数: 0
Biopolymer Based Nanoparticles: Isolation, Characterization, and Applications 基于生物聚合物的纳米颗粒:分离、表征和应用
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.044044
The budding field of Nanotechnology has become one of the most popular areas of interest for current research and development, especially in the research areas of polymer science and technology. Natural Polysaccharide polymers are found abundant in nature and are now frequently been used for the preparation of many nanomaterials. When compared to the synthetic polymers available, natural biopolymers are more biocompatible, biodegradable, and renewable in nature. The production of Nanomaterials from biopolymers has eventually improved the properties of the Nano-products developed. Nature has provided many different varieties of natural polymers; one such plant-derived polymer is the plant Gum exudate. Gums are commonly exuded by plants at very small quantities, forming conspicuous incrustations. These Natural gums exudates are polysaccharides. They are chemically inert, biocompatible, biodegradable, and sustainable in nature. In this study, plant gum-based biopolymers were used for the synthesis of nanoparticles. The natural gum exudates were collected from the plants' Araucaria heterophylla, Azadirachta indica. The obtained biopolymers were purified by the solvent extraction method and were characterized by UV-Vis Spectroscopy, FTIR, and TGA. Further, the purified biopolymers were subjected to the encapsulation of drugs (as Nanocarrier) and coating for SPIONs (superparamagnetic iron oxide nanoparticles). The synthesized nanoparticles were characterized by UV-Vis Spectroscopy, FTIR, SEM, EDAX, TEM, and AFM. The biopolymer-based nanocarriers were about 200nm in size and were utilized for effective drug delivery studies using MCF-7 Breast cancer lines. Finally, the biopolymer encapsulated SPIONs particles of size around 40nm were used for water treatment studies - removing the heavy metal chromium as well as mosquito larvae from water. Thus, the nano-products developed were found to be more eco-friendly, efficient, and also cost-effective.
纳米技术这一新兴领域已经成为当前研究和发展中最受欢迎的领域之一,特别是在聚合物科学和技术的研究领域。天然多糖聚合物在自然界中是丰富的,现在经常被用于制备许多纳米材料。与现有的合成聚合物相比,天然生物聚合物具有更强的生物相容性、可生物降解性和可再生性。生物聚合物纳米材料的生产最终改善了纳米产品的性能。大自然提供了许多不同种类的天然聚合物;其中一种植物衍生的聚合物是植物胶渗出物。树胶通常由植物少量渗出,形成明显的表皮。这些天然胶质渗出物是多糖。它们具有化学惰性、生物相容性、可生物降解性和可持续性。在本研究中,植物胶基生物聚合物用于纳米颗粒的合成。天然树胶渗出液采集自芥蓝、印楝等植物。采用溶剂萃取法对所得生物聚合物进行了纯化,并用紫外可见光谱、红外光谱和热重分析仪对所得生物聚合物进行了表征。此外,纯化的生物聚合物进行药物包封(作为纳米载体)和SPIONs(超顺磁性氧化铁纳米颗粒)涂层。采用紫外可见光谱、FTIR、SEM、EDAX、TEM和AFM对合成的纳米颗粒进行了表征。基于生物聚合物的纳米载体尺寸约为200nm,用于MCF-7乳腺癌细胞系的有效药物递送研究。最后,将40nm大小的生物聚合物包裹的SPIONs颗粒用于水处理研究-去除水中的重金属铬和蚊子幼虫。因此,所开发的纳米产品更加环保、高效,并且具有成本效益。
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引用次数: 0
Antidiabetic Activity of Fish Oil from Sardinella longiceps Against Wound Pathogen in Diabetic Patients 长尾沙丁鱼鱼油对糖尿病患者伤口病原菌的抗糖尿病作用
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.058058
Diabetes mellitus is a serious problem worldwide. It arises due to the failure of the pancreas to secrete insulin, which is mandatory to maintain a normal blood sugar level. Deficient insulin action cause diabetes. In this study, oil was extracted from Sardinella longiceps by appropriate techniques. The antidiabetic activity was examined by an alloxan-induced method. It resulted in decreased blood sugar levels.
糖尿病是一个世界性的严重问题。它是由于胰腺不能分泌胰岛素而引起的,而胰岛素是维持正常血糖水平所必需的。胰岛素作用不足会导致糖尿病。本研究采用适当的工艺从长尾沙丁鱼中提取油脂。采用四氧嘧啶诱导法检测其抗糖尿病活性。它导致血糖水平降低。
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引用次数: 0
Influence of Probiotic Bacteria Pediococcus sp and Bifidobacterium sp on Immune System 益生菌Pediococcus sp和双歧杆菌sp对免疫系统的影响
Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.064064
Probiotic organisms influence the intestinal health. It helps in the maturation of the immune system, the development of normal intestinal morphology, maintains the load of intestinal flora, etc. It prevents the attachment of pathogenic microorganisms and the entry of allergens into the intestine. Probiotics improve the immune status of the individuals, which was detected by checking the levels of IgG and IgM for E. coli infection on an experimental animal fed with a probiotic drink prepared using Pediococcus sp and Bifidobacterium sp for one month. Control animals showed an increase in the level of Immunoglobulins. Both IgG and IgM, indicated the level of infection, whereas test animals did not show any increase in the level of Immunoglobulins.
益生菌影响肠道健康。它有助于免疫系统的成熟,正常肠道形态的发育,维持肠道菌群的负荷等。它可以防止致病微生物的附着和过敏原进入肠道。益生菌可以提高个体的免疫状态,通过检测实验动物大肠杆菌感染的IgG和IgM水平,实验动物喂食由Pediococcus sp和Bifidobacterium sp配制的益生菌饮料一个月。对照动物的免疫球蛋白水平增加。IgG和IgM均显示感染水平,而试验动物的免疫球蛋白水平未显示任何增加。
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引用次数: 0
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Proceedings. International Meshing Roundtable
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