Pub Date : 2018-01-01DOI: 10.18488/JOURNAL.57.2018.71.17.24
Getnet Belay, Tigist Minyamer, Getinet Masresha
Bubugn is a cereal based traditional fermented Ethiopian low alcoholic beverage. It is one of the traditional fermented beverages used for a drink of holidays, wedding ceremony and also used as a source of income. The aims of this study were to determine the microbiology, microbial contaminants and physicochemical characteristics of Bubugn. Nine Samples of Bubugn were collected from Gondar town in three district areas; Azezo (A), Arada (B) and Kebele 18 (C). Microbial counts and physicochemical analysis were enumerated using standard microbiological methods. The mean value of the pH of the samples was 4.20 + 0.14; and the mean value of moisture content was 45. 79 +1.35. The mean crude fat, ash and ethanol contents of Bubugn were 6.67 + 0.16, 4.47+ 0.40 and 1.79 + 0.13, respectively. The mean number of total mesophilic aerobic bacteria, yeasts and molds and lactic acid bacteria of Bubugn were 3.74×105 cfu/ml, 5.68 ×106 cfu/ml and 7.52×105 cfu/ml respectively. Bubugn samples were contaminated by Shigella species, E. coli and S. aureus which could be due to poor hygienic conditions related to washing of preparation material, use of contaminated water and poor personal and domestic hygiene. The collected Bubugn samples were contaminated by different microorganisms and therefore, there should be the development of an advanced technique to improve quality.
{"title":"The Microbial Profile and Contaminants of Bubugn, An Ethiopian House Hould Fermented Beverage","authors":"Getnet Belay, Tigist Minyamer, Getinet Masresha","doi":"10.18488/JOURNAL.57.2018.71.17.24","DOIUrl":"https://doi.org/10.18488/JOURNAL.57.2018.71.17.24","url":null,"abstract":"Bubugn is a cereal based traditional fermented Ethiopian low alcoholic beverage. It is one of the traditional fermented beverages used for a drink of holidays, wedding ceremony and also used as a source of income. The aims of this study were to determine the microbiology, microbial contaminants and physicochemical characteristics of Bubugn. Nine Samples of Bubugn were collected from Gondar town in three district areas; Azezo (A), Arada (B) and Kebele 18 (C). Microbial counts and physicochemical analysis were enumerated using standard microbiological methods. The mean value of the pH of the samples was 4.20 + 0.14; and the mean value of moisture content was 45. 79 +1.35. The mean crude fat, ash and ethanol contents of Bubugn were 6.67 + 0.16, 4.47+ 0.40 and 1.79 + 0.13, respectively. The mean number of total mesophilic aerobic bacteria, yeasts and molds and lactic acid bacteria of Bubugn were 3.74×105 cfu/ml, 5.68 ×106 cfu/ml and 7.52×105 cfu/ml respectively. Bubugn samples were contaminated by Shigella species, E. coli and S. aureus which could be due to poor hygienic conditions related to washing of preparation material, use of contaminated water and poor personal and domestic hygiene. The collected Bubugn samples were contaminated by different microorganisms and therefore, there should be the development of an advanced technique to improve quality.","PeriodicalId":91506,"journal":{"name":"International journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67986640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.18488/JOURNAL.57.2018.71.64.69
Luke Chinaru Nwosu, G. Ugagu, A. Eluwa, T. Arotolu, O. Obi, Daniel D. Adanyi, V. Okereke, Vivian A. Azoro, Kalu U. Ukpai, Gospel U. Nzewuihe, I. A. Lawal, O. A. Uwalaka
Powders from five elite plant materials (nutmeg, Myristica fragrans; red hot chilli pepper, Capsicum annuum; ginger rhizome, Zingiber officinale; black pepper, Piper nigrum and alligator pepper, Aframomum melegueta) at 1.5 % w/w concentration were assessed against cowpea bruchid, Callosobruchus maculatus infestations on stored cowpea seeds under laboratory environmental conditions in three agro-ecological zones in Nigeria by members of the Research Group. The conventional synthetic insecticide, permethrin was included as standard check, while untreated seeds served as the control. Adult mortality, oviposition rate and adult emergence were the indices considered in the experiments. For the first three days of exposure, black pepper (P. nigrum) powder caused the highest percentage mortality (68.8, 78.0 and 83.8 % respectively) of the bruchid adults and differed significantly (P
{"title":"Insecticidal Activities of Five Medicinal Plant Materials against Callosobruchus Maculatus Fabricius (Coleoptera: Chrysomelidae) Infesting Cowpea Seeds in Storage","authors":"Luke Chinaru Nwosu, G. Ugagu, A. Eluwa, T. Arotolu, O. Obi, Daniel D. Adanyi, V. Okereke, Vivian A. Azoro, Kalu U. Ukpai, Gospel U. Nzewuihe, I. A. Lawal, O. A. Uwalaka","doi":"10.18488/JOURNAL.57.2018.71.64.69","DOIUrl":"https://doi.org/10.18488/JOURNAL.57.2018.71.64.69","url":null,"abstract":"Powders from five elite plant materials (nutmeg, Myristica fragrans; red hot chilli pepper, Capsicum annuum; ginger rhizome, Zingiber officinale; black pepper, Piper nigrum and alligator pepper, Aframomum melegueta) at 1.5 % w/w concentration were assessed against cowpea bruchid, Callosobruchus maculatus infestations on stored cowpea seeds under laboratory environmental conditions in three agro-ecological zones in Nigeria by members of the Research Group. The conventional synthetic insecticide, permethrin was included as standard check, while untreated seeds served as the control. Adult mortality, oviposition rate and adult emergence were the indices considered in the experiments. For the first three days of exposure, black pepper (P. nigrum) powder caused the highest percentage mortality (68.8, 78.0 and 83.8 % respectively) of the bruchid adults and differed significantly (P","PeriodicalId":91506,"journal":{"name":"International journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67987344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antihyperglycemic Effects of Aqueous Extracts of Zingiber Officinale, Cinnamonum Zeylanicum and their Combination in Experimental Rats","authors":"Muhammad Usman, A. J. Alhassan, Hauwa Sa ad","doi":"10.18488/journal.57.2018.71.25.30","DOIUrl":"https://doi.org/10.18488/journal.57.2018.71.25.30","url":null,"abstract":"Article History Received: 17 July 2018 Revised: 24 August 2018 Accepted: 27 September 2018 Published: 7 November 2018","PeriodicalId":91506,"journal":{"name":"International journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67987143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-06-21DOI: 10.1504/IJBT.2017.10004750
Z. Kulichova, H. Coumou, Mahaletchumy Arujanan, C. Wehrmann, P. Osseweijer
Scientists have always played an important role in informing policy decisions. However, many controversial policy problems regarding science and technology, such as agricultural biotechnology, are often characterised by low value consensus and high level of complexity. In these circumstances various policy actors legitimate their policy preferences using science. In this article, we challenge the linear model of science and policy and argue that the stakeholder model of science in policy is more appropriate for governance of controversial policy problems regarding science and technology. We build our argument on available literature and empirical data from interviews and two online surveys. We choose agricultural biotechnology as the case study to illustrate scientists' perception about their role in policy-making. Our study illustrates that agricultural biotechnology scientists sympathise with the stakeholder model of science and policy. However, there is a gap between perceived ideal role for scientists in policy-making and the role, which these scientists actually take.
{"title":"The role of scientists in policy-making regarding agricultural biotechnology: from traditional to alternative views","authors":"Z. Kulichova, H. Coumou, Mahaletchumy Arujanan, C. Wehrmann, P. Osseweijer","doi":"10.1504/IJBT.2017.10004750","DOIUrl":"https://doi.org/10.1504/IJBT.2017.10004750","url":null,"abstract":"Scientists have always played an important role in informing policy decisions. However, many controversial policy problems regarding science and technology, such as agricultural biotechnology, are often characterised by low value consensus and high level of complexity. In these circumstances various policy actors legitimate their policy preferences using science. In this article, we challenge the linear model of science and policy and argue that the stakeholder model of science in policy is more appropriate for governance of controversial policy problems regarding science and technology. We build our argument on available literature and empirical data from interviews and two online surveys. We choose agricultural biotechnology as the case study to illustrate scientists' perception about their role in policy-making. Our study illustrates that agricultural biotechnology scientists sympathise with the stakeholder model of science and policy. However, there is a gap between perceived ideal role for scientists in policy-making and the role, which these scientists actually take.","PeriodicalId":91506,"journal":{"name":"International journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47218334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-06-21DOI: 10.1504/IJBT.2017.10005655
Charles Matlou, M. Antwi, C. Chagwiza
The use of Bacillus thuringiensis (Bt) maize gene was commercialised by the South African Government in 1998. This gene makes the maize resistant to maize stem borer thereby improving production. However, despite the efficiency of any given technology, there are always factors which encourage or discourage farmers to adopt and these need to be identified. This study was therefore carried out to determine the factors that influence adoption of Bt maize among 121 developing maize farmers in Gauteng Province, South Africa. Using a logistic regression model, the findings show that a set of socio-economic factors influence farmers' decision to adopt Bt maize technology from 2011 to 2014 maize production season. Policymakers should pay closer attention to such factors to stimulate the scaling up in adoption of Bt maize technology and ensure its sustainability. This will help improve productivity and curb maize shortages currently facing the country and the world over.
{"title":"Determinants of maize farmers' adoption of Bt maize in Gauteng Province of South Africa","authors":"Charles Matlou, M. Antwi, C. Chagwiza","doi":"10.1504/IJBT.2017.10005655","DOIUrl":"https://doi.org/10.1504/IJBT.2017.10005655","url":null,"abstract":"The use of Bacillus thuringiensis (Bt) maize gene was commercialised by the South African Government in 1998. This gene makes the maize resistant to maize stem borer thereby improving production. However, despite the efficiency of any given technology, there are always factors which encourage or discourage farmers to adopt and these need to be identified. This study was therefore carried out to determine the factors that influence adoption of Bt maize among 121 developing maize farmers in Gauteng Province, South Africa. Using a logistic regression model, the findings show that a set of socio-economic factors influence farmers' decision to adopt Bt maize technology from 2011 to 2014 maize production season. Policymakers should pay closer attention to such factors to stimulate the scaling up in adoption of Bt maize technology and ensure its sustainability. This will help improve productivity and curb maize shortages currently facing the country and the world over.","PeriodicalId":91506,"journal":{"name":"International journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47374991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-06-21DOI: 10.1504/IJBT.2017.10004751
Alessandro Marra, Paola Antonelli, C. Pozzi, Alessandro Sarra
Biotechnology is a fast growing market led by a multitude of start-ups and SMEs, extraordinarily active in research and development and technological innovation for industrial purposes. Given the high fragmentation of the industry and the radical disruptive nature of the innovative process, any empirical investigation aimed at identifying biotech R&D and industry applications makes a valuable contribution. Moreover, given the spatial concentration of biotech companies, often at the metropolitan level, the detection of specialised aggregates based on firms' technological and market proximity offers an interesting perspective of study on clusters. The paper employs network analysis using metadata to determine emerging firms' specialisations and capture technological and market complementarities, and emphasises the latter as drivers of biotech aggregates, in San Diego, CA, and Cambridge, MA, two large cities at the industry forefront. Results show that San Diego has the larger and less interconnected network, compared to Cambridge, although existing clusters, specialisations and industry applications seem to be complementary and better interconnected.
{"title":"Biotech specialisations and metropolitan clusters in San Diego, CA, and Cambridge, MA - a network analysis using metadata","authors":"Alessandro Marra, Paola Antonelli, C. Pozzi, Alessandro Sarra","doi":"10.1504/IJBT.2017.10004751","DOIUrl":"https://doi.org/10.1504/IJBT.2017.10004751","url":null,"abstract":"Biotechnology is a fast growing market led by a multitude of start-ups and SMEs, extraordinarily active in research and development and technological innovation for industrial purposes. Given the high fragmentation of the industry and the radical disruptive nature of the innovative process, any empirical investigation aimed at identifying biotech R&D and industry applications makes a valuable contribution. Moreover, given the spatial concentration of biotech companies, often at the metropolitan level, the detection of specialised aggregates based on firms' technological and market proximity offers an interesting perspective of study on clusters. The paper employs network analysis using metadata to determine emerging firms' specialisations and capture technological and market complementarities, and emphasises the latter as drivers of biotech aggregates, in San Diego, CA, and Cambridge, MA, two large cities at the industry forefront. Results show that San Diego has the larger and less interconnected network, compared to Cambridge, although existing clusters, specialisations and industry applications seem to be complementary and better interconnected.","PeriodicalId":91506,"journal":{"name":"International journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45079382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-06-21DOI: 10.1504/IJBT.2017.10004752
C. Cormick, L. Romanach, Owen Craig
The Holy Grail of public attitude research towards agricultural biotechnology is too often to try and find a simple single statistic that will capture public support or rejection of GM foods and crops, often to suit political or media needs. Public attitude research, however, shows that opinions are too complex and variable to be captured by a single statistic and, indeed, more complex forms of analyses are needed to better capture these complexities and variabilities. The Australian Department of Innovation sought to capture such complexities through a large-scale multiple-methodology study that included: longitudinal data, measuring attitudes across a broad scale of support or rejection, seeking relative comparisons of attitudes to other food types, analysing what would change attitudes and, vitally, examining the values that influence attitudes. Taken together the findings allowed for a values segmentation of the public, into four key segments, with distinct homogenous attitudes and values that demonstrate there is not a single 'Holy Grail figure' that defines public support for agricultural biotechnology. A deeper understanding of the distinct values segments, however, allows for not just a better understanding of the diversity of views that exist, but a better understanding of what messages will most aligned with those segments to best reach them.
{"title":"Searching for the Holy Grail: untangling the complexity of public attitudes towards agricultural biotechnology","authors":"C. Cormick, L. Romanach, Owen Craig","doi":"10.1504/IJBT.2017.10004752","DOIUrl":"https://doi.org/10.1504/IJBT.2017.10004752","url":null,"abstract":"The Holy Grail of public attitude research towards agricultural biotechnology is too often to try and find a simple single statistic that will capture public support or rejection of GM foods and crops, often to suit political or media needs. Public attitude research, however, shows that opinions are too complex and variable to be captured by a single statistic and, indeed, more complex forms of analyses are needed to better capture these complexities and variabilities. The Australian Department of Innovation sought to capture such complexities through a large-scale multiple-methodology study that included: longitudinal data, measuring attitudes across a broad scale of support or rejection, seeking relative comparisons of attitudes to other food types, analysing what would change attitudes and, vitally, examining the values that influence attitudes. Taken together the findings allowed for a values segmentation of the public, into four key segments, with distinct homogenous attitudes and values that demonstrate there is not a single 'Holy Grail figure' that defines public support for agricultural biotechnology. A deeper understanding of the distinct values segments, however, allows for not just a better understanding of the diversity of views that exist, but a better understanding of what messages will most aligned with those segments to best reach them.","PeriodicalId":91506,"journal":{"name":"International journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44140842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.18488/journal.57.2017.61.1.10
Istifanus Boyi Maikasuwa, C. Iheukwumere, I. O. Ogbonna
Some grains were analysed for their nutrient compositions for use in the formulation of bacteriological media in consideration of cheap and effective alternative to the conventional media. The media were respectively formulated using Acha (Digitaria exilis), Maize (Zea mays), Rice (Oryza sativa), Guinea corn (Sorghum species) and Millet (Pennisetum glaucum). Proximate analyses of the grains showed reasonable amounts of carbohydrates (55.0 to 73.9%), moisture contents (9.92 to 11.25%) and crude proteins (7.39 to 11.77%). Crude fibre and ash had the least % in the five grains used. Amongst the macro-elements, carbon is the most abundant followed by nitrogen. Using atomic absorption spectrophotometer (AAS), Zn, Co, Cu and Mo were not detectable in the grains, whereas Mn was present in minute quantities (= 0.015%). All the bacteria assessed grew on the formulated media. Statistical analysis of the data indicated homogeneity in growth rates of the test bacteria on most of the media formulated. However higher microbial counts observed on millet extract agar (MIEA) suggests that the medium could serve as an alternative growth medium for bacteria in place of the conventional nutrient agar (NA) with reduced cost.
{"title":"Nutrient Compositions of Different Grains for Use in the Formulation of Bacteriological Media","authors":"Istifanus Boyi Maikasuwa, C. Iheukwumere, I. O. Ogbonna","doi":"10.18488/journal.57.2017.61.1.10","DOIUrl":"https://doi.org/10.18488/journal.57.2017.61.1.10","url":null,"abstract":"Some grains were analysed for their nutrient compositions for use in the formulation of bacteriological media in consideration of cheap and effective alternative to the conventional media. The media were respectively formulated using Acha (Digitaria exilis), Maize (Zea mays), Rice (Oryza sativa), Guinea corn (Sorghum species) and Millet (Pennisetum glaucum). Proximate analyses of the grains showed reasonable amounts of carbohydrates (55.0 to 73.9%), moisture contents (9.92 to 11.25%) and crude proteins (7.39 to 11.77%). Crude fibre and ash had the least % in the five grains used. Amongst the macro-elements, carbon is the most abundant followed by nitrogen. Using atomic absorption spectrophotometer (AAS), Zn, Co, Cu and Mo were not detectable in the grains, whereas Mn was present in minute quantities (= 0.015%). All the bacteria assessed grew on the formulated media. Statistical analysis of the data indicated homogeneity in growth rates of the test bacteria on most of the media formulated. However higher microbial counts observed on millet extract agar (MIEA) suggests that the medium could serve as an alternative growth medium for bacteria in place of the conventional nutrient agar (NA) with reduced cost.","PeriodicalId":91506,"journal":{"name":"International journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67986332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.18488/JOURNAL.57.2017.61.11.18
Amakoé Adjanke, K. Tona, I. Toko, M. Gbéassor
An experiment was conducted to determine the effect of treated palm kernel meal in diets on production parameters of Nile tilapia Oreochromis niloticus reared in concrete tanks. Fingerlings of Tilapia with an average initial weight 6.22 ± 0.25 g and an average size 7.58 ± 0.21 cm were fed four isonitrogenous diets each containing 30% palm kernel meal treated one-hour or not. So we have NT (untreated), DW (dipped in water), CW (cooked in water) and SW (steamed). These diets were compared with a commercial fish feed, Raanan (RA), all about 32% crude protein. After 8 weeks of experiment the final body weight varied between 29.57 and 43.01 g according to the tested treatments. The best growth rate and food conversion ratio were obtained with diet CW containing palm kernel meal cooked in water with specific growth rate (SGR) of 3.24 %/d and food conversion ratio (FCR) of 1.97 against a SGR of 3.44%/d and a FCR of 1.72 obtained with commercial feed (RA). Moreover, the tested diets do not seem to have any effect on the intestine size but the cooking seems to act on fish liver weight and body composition. The production parameters were improved with the diet containing palm kernel meal cooked in water which seems to be the interest food for O. niloticus on growing.
{"title":"Effects of Technological Treatments of Dietary Palm Kernel Meal on Feed Intake, Growth and Body Composition of Oreochromis Niloticus Reared in Concrete Tanks","authors":"Amakoé Adjanke, K. Tona, I. Toko, M. Gbéassor","doi":"10.18488/JOURNAL.57.2017.61.11.18","DOIUrl":"https://doi.org/10.18488/JOURNAL.57.2017.61.11.18","url":null,"abstract":"An experiment was conducted to determine the effect of treated palm kernel meal in diets on production parameters of Nile tilapia Oreochromis niloticus reared in concrete tanks. Fingerlings of Tilapia with an average initial weight 6.22 ± 0.25 g and an average size 7.58 ± 0.21 cm were fed four isonitrogenous diets each containing 30% palm kernel meal treated one-hour or not. So we have NT (untreated), DW (dipped in water), CW (cooked in water) and SW (steamed). These diets were compared with a commercial fish feed, Raanan (RA), all about 32% crude protein. After 8 weeks of experiment the final body weight varied between 29.57 and 43.01 g according to the tested treatments. The best growth rate and food conversion ratio were obtained with diet CW containing palm kernel meal cooked in water with specific growth rate (SGR) of 3.24 %/d and food conversion ratio (FCR) of 1.97 against a SGR of 3.44%/d and a FCR of 1.72 obtained with commercial feed (RA). Moreover, the tested diets do not seem to have any effect on the intestine size but the cooking seems to act on fish liver weight and body composition. The production parameters were improved with the diet containing palm kernel meal cooked in water which seems to be the interest food for O. niloticus on growing.","PeriodicalId":91506,"journal":{"name":"International journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67986619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-11-03DOI: 10.18488/JOURNAL.57/2016.5.4/57.4.52.61
Fatemeh Ghorbaniparsa, H. Ofoghi
Calcitonin (CT), a 32 amino acid polypeptide hormone is a powerful and specific inhibitor of bone resorption and is used to treat several human diseases like hypercalcemia and osteoporosis. To date, many pharmaceutical proteins of mammalian origin have been synthesized in plants. To increase the production level of heterologous proteins in plants, strategies such as choice of stronger promoters and optimization of codon usage are of major concern. In this study, a human calcitonin (hCT) gene, driven by two different promoters (Patatin Class I and Cauliflower mosaic virus 35S) was expressed in two types of potato's cultivars (cvs) Kardal and Marfona plants, using Agrobacterium-mediated transformation. The transgenic plants were analyzed by molecular methods and hCT concentration was determined by quantitative EASIA. The results showed the localization of hCT production in Kardal potato tubers led to 0.7% of total soluble proteins whiles total soluble protein was 0.2% when CaMV 35S promoter was deployed, these results of Marfona when used Patatin Class I and Cauliflower mosaic virus 35S promoters were 0.3% of the total soluble protein and 0.1% of the total soluble protein in the respect. Data were analyzed by SPSS software using analytical statistics. There was significant difference in mean score of hCT production when two different promoters and two different cultivars were used. These results showed that organ specific expression in potato led to nearly 3 fold higher hCT accumulation than constitutive expression and Kardal cultivar expressed hCT about 2 times higher than Marfona cultivar.
降钙素(CT)是一种32个氨基酸的多肽激素，是一种强大的特异性骨吸收抑制剂，用于治疗多种人类疾病，如高钙血症和骨质疏松症。迄今为止，许多源自哺乳动物的药用蛋白已经在植物中合成。为了提高植物中异源蛋白的产生水平，选择更强的启动子和优化密码子的使用等策略是人们关注的主要问题。本研究利用农杆菌介导的转化技术，在两种马铃薯品种Kardal和Marfona植株上表达了一个人类降钙素(hCT)基因，该基因由两种不同的启动子(Patatin Class I和花菜花叶病毒35S)驱动。采用分子分析方法对转基因植株进行分析，采用定量EASIA法测定hCT浓度。结果表明，在卡达尔马铃薯块茎中定位产生hCT，总可溶性蛋白为0.7%，而在CaMV 35S启动子中定位产生的总可溶性蛋白为0.2%，在使用Patatin I类启动子和花椰菜花叶病毒35S启动子时定位产生的总可溶性蛋白为0.3%，在总可溶性蛋白中定位产生的总可溶性蛋白为0.1%。数据采用SPSS软件进行分析统计。使用两种不同的启动子和两种不同的品种，hCT生产的平均得分有显著差异。结果表明，在马铃薯中，器官特异性表达导致hCT的积累量比构成型表达量高出近3倍，卡达尔品种的hCT表达量比马弗纳品种高出约2倍。
{"title":"Comparing Patatin Class I and Camv 35s Promoters in Expression of Human Calcitonin Gene in Potato (Solanum Tuberosum Cvs. Kardal And Marfona)","authors":"Fatemeh Ghorbaniparsa, H. Ofoghi","doi":"10.18488/JOURNAL.57/2016.5.4/57.4.52.61","DOIUrl":"https://doi.org/10.18488/JOURNAL.57/2016.5.4/57.4.52.61","url":null,"abstract":"Calcitonin (CT), a 32 amino acid polypeptide hormone is a powerful and specific inhibitor of bone resorption and is used to treat several human diseases like hypercalcemia and osteoporosis. To date, many pharmaceutical proteins of mammalian origin have been synthesized in plants. To increase the production level of heterologous proteins in plants, strategies such as choice of stronger promoters and optimization of codon usage are of major concern. In this study, a human calcitonin (hCT) gene, driven by two different promoters (Patatin Class I and Cauliflower mosaic virus 35S) was expressed in two types of potato's cultivars (cvs) Kardal and Marfona plants, using Agrobacterium-mediated transformation. The transgenic plants were analyzed by molecular methods and hCT concentration was determined by quantitative EASIA. The results showed the localization of hCT production in Kardal potato tubers led to 0.7% of total soluble proteins whiles total soluble protein was 0.2% when CaMV 35S promoter was deployed, these results of Marfona when used Patatin Class I and Cauliflower mosaic virus 35S promoters were 0.3% of the total soluble protein and 0.1% of the total soluble protein in the respect. Data were analyzed by SPSS software using analytical statistics. There was significant difference in mean score of hCT production when two different promoters and two different cultivars were used. These results showed that organ specific expression in potato led to nearly 3 fold higher hCT accumulation than constitutive expression and Kardal cultivar expressed hCT about 2 times higher than Marfona cultivar.","PeriodicalId":91506,"journal":{"name":"International journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67989544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：