Vera Stanković, N. Kuzmanović, E. Kabaš, S. Vukojičić, D. Lakušić, S. Jovanovic
Monitoring the presence of invasive species in natural wetlands is crucial for numerous reasons, including their negative impact on biodiversity, conservation and the ecosystem services provided by these important fragile areas. The aim of this paper was to investigate the presence and distribution of the highly invasive liana Echinocystis lobata (wild cucumber), and to determine its coenological relations with the native plant species in the Ramsar sites of the southern part of the Pannonian Plain (the southeastern part of Central Europe, northern Serbia). We conducted the phytocenological research of the selected protected riparian areas in detail over a six-year period (2011-2015 and 2020). This study revealed the significant presence of the highly invasive species E. lobata in the studied sites. Wild cucumbers were found in 146 plots in four out of the seven investigated areas. Cluster analyses distinguished four groups of relev?s dominated by E. lobata, which were described, and their dominant and constant species were identified. Habitat type identification was done, and the habitat preferences of E. lobata were determined. The spatial distribution of the four groups is such that they occur in a number of different habitat types or local communities. The species pool which makes up each of these four groups is thus geographically larger than the local community under study, so we can treat them as independently established and well-defined new community assemblages. Plant communities dominated by the invasive species E. lobata have not been described previously. Therefore, this work provides new data, and contributes to the further research and comparative analyses needed to describe invasive plant communities dominated by wild cucumber. In addition, the identification of habitat types which are most occupied by E. lobata is very important for managers of protected areas, as it allows them to better control and remove this highly invasive species, but also to prevent its further spread.
{"title":"Established stands of the highly invasive Echinocystis lobata on the Ramsar sites of the southern part of the Pannonian plain","authors":"Vera Stanković, N. Kuzmanović, E. Kabaš, S. Vukojičić, D. Lakušić, S. Jovanovic","doi":"10.2298/botserb2202197s","DOIUrl":"https://doi.org/10.2298/botserb2202197s","url":null,"abstract":"Monitoring the presence of invasive species in natural wetlands is crucial for numerous reasons, including their negative impact on biodiversity, conservation and the ecosystem services provided by these important fragile areas. The aim of this paper was to investigate the presence and distribution of the highly invasive liana Echinocystis lobata (wild cucumber), and to determine its coenological relations with the native plant species in the Ramsar sites of the southern part of the Pannonian Plain (the southeastern part of Central Europe, northern Serbia). We conducted the phytocenological research of the selected protected riparian areas in detail over a six-year period (2011-2015 and 2020). This study revealed the significant presence of the highly invasive species E. lobata in the studied sites. Wild cucumbers were found in 146 plots in four out of the seven investigated areas. Cluster analyses distinguished four groups of relev?s dominated by E. lobata, which were described, and their dominant and constant species were identified. Habitat type identification was done, and the habitat preferences of E. lobata were determined. The spatial distribution of the four groups is such that they occur in a number of different habitat types or local communities. The species pool which makes up each of these four groups is thus geographically larger than the local community under study, so we can treat them as independently established and well-defined new community assemblages. Plant communities dominated by the invasive species E. lobata have not been described previously. Therefore, this work provides new data, and contributes to the further research and comparative analyses needed to describe invasive plant communities dominated by wild cucumber. In addition, the identification of habitat types which are most occupied by E. lobata is very important for managers of protected areas, as it allows them to better control and remove this highly invasive species, but also to prevent its further spread.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"94 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76425527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
X. Yuan, Cuiyu Li, Yi Tang, Zhiyu Chen, Chun-Wen Huang
Grassland desertification seriously destroys the structure of the soil microbial communities and further accelerates the deterioration of grassland. In this study, functional microbial agents were sprayed on degraded grasslands and studied by means of real-time fluorescence quantitative PCR and high-throughput sequencing methods. The aim was to investigate the effects of microbial agents on the soil microbial community structure and soil remediation functions in different degraded grasslands (LDG: light desertification grassland, MDG: medium desertification grassland, and HDG: heavy desertification grassland). The results showed that after treatment with the microbial agents, bacterial abundance increased by 96.24% (LDG), 95.19% (MDG), and 93.47% (HDG), respectively, and fungal abundance increased by 85.77% (LDG), 95.85% (MDG) and 22.49% (HDG), respectively. Further, with the colonisation and acclimatisation of foreign functional microorganisms, the microbial agents greatly influenced the structure of the soil microbial community, increased the microbial diversity index, and significantly changed the microbial community composition. The application of the microbial agents did not only improve the forage yield and quality, but also guided the soil restoration, improved the soil water content of sandy grassland, adjusted the soil pH, significantly increased the content of soil organic matter, total nitrogen, and total phosphorus, and also inhibited the growth of soil-borne pathogens. Our findings provide new ideas and guidance for the management of degraded grassland.
{"title":"The effects of functional microbial agents on the soil microbial communities of high-frigid grassland under desertification in Northwest Sichuan","authors":"X. Yuan, Cuiyu Li, Yi Tang, Zhiyu Chen, Chun-Wen Huang","doi":"10.2298/botserb2202259y","DOIUrl":"https://doi.org/10.2298/botserb2202259y","url":null,"abstract":"Grassland desertification seriously destroys the structure of the soil microbial communities and further accelerates the deterioration of grassland. In this study, functional microbial agents were sprayed on degraded grasslands and studied by means of real-time fluorescence quantitative PCR and high-throughput sequencing methods. The aim was to investigate the effects of microbial agents on the soil microbial community structure and soil remediation functions in different degraded grasslands (LDG: light desertification grassland, MDG: medium desertification grassland, and HDG: heavy desertification grassland). The results showed that after treatment with the microbial agents, bacterial abundance increased by 96.24% (LDG), 95.19% (MDG), and 93.47% (HDG), respectively, and fungal abundance increased by 85.77% (LDG), 95.85% (MDG) and 22.49% (HDG), respectively. Further, with the colonisation and acclimatisation of foreign functional microorganisms, the microbial agents greatly influenced the structure of the soil microbial community, increased the microbial diversity index, and significantly changed the microbial community composition. The application of the microbial agents did not only improve the forage yield and quality, but also guided the soil restoration, improved the soil water content of sandy grassland, adjusted the soil pH, significantly increased the content of soil organic matter, total nitrogen, and total phosphorus, and also inhibited the growth of soil-borne pathogens. Our findings provide new ideas and guidance for the management of degraded grassland.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"45 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78680114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Sabovljević, G. Tomović, J. Pantović, Sanja Djurovic, Uroš Buzurović, T. Denchev, C. Denchev, Petya Boycheva, T. Dimitrova, Aleksandra Marković, A. Sabovljevic, S. Ștefănuț, C. Bîrsan, Elvedin Šabanović, V. Djordjević, M. Niketić, Sanja Sovran, E. Masic, D. Stoykov, B. Papp, B. Assyov, M. Slavova
This paper presents new records and noteworthy data on the following taxa in SE Europe and adjacent regions: red algae Lemanea fucina and Paralemanea annulata, parasitic fungus Anthracoidea pratensis, saprotrophic fungi Cyathus olla, Massaria campestris, and Xylaria sicula, stonewort Chara canescens, liverworts Gymnomitrion commutatum and Porella baueri, moss Acaulon triquetrum, monocots Anacamptis laxiflora, Cephalanthera damasonium, and Himantoglossum robertianum and dicot Jacobaea othonnae are given within SE Europe and adjacent regions.
{"title":"New records and noteworthy data of plants, algae and fungi in SE Europe and adjacent regions, 9","authors":"M. Sabovljević, G. Tomović, J. Pantović, Sanja Djurovic, Uroš Buzurović, T. Denchev, C. Denchev, Petya Boycheva, T. Dimitrova, Aleksandra Marković, A. Sabovljevic, S. Ștefănuț, C. Bîrsan, Elvedin Šabanović, V. Djordjević, M. Niketić, Sanja Sovran, E. Masic, D. Stoykov, B. Papp, B. Assyov, M. Slavova","doi":"10.2298/botserb2202311s","DOIUrl":"https://doi.org/10.2298/botserb2202311s","url":null,"abstract":"This paper presents new records and noteworthy data on the following taxa in SE Europe and adjacent regions: red algae Lemanea fucina and Paralemanea annulata, parasitic fungus Anthracoidea pratensis, saprotrophic fungi Cyathus olla, Massaria campestris, and Xylaria sicula, stonewort Chara canescens, liverworts Gymnomitrion commutatum and Porella baueri, moss Acaulon triquetrum, monocots Anacamptis laxiflora, Cephalanthera damasonium, and Himantoglossum robertianum and dicot Jacobaea othonnae are given within SE Europe and adjacent regions.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"7 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91112700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Ćosić, M. Sabovljević, B. Papp, Z. Giba, J. Šinžar-Sekulić, A. Sabovljevic, Milorad M Vujičić
The rare moss species Hennediella heimii (Pottiaceae) was established in in vitro culture. Various treatments were tested to achieve axenical cultures. The most effective sterilising procedure was NaDCC treatment of sporophytes, keeping the spore viability and giving high disposal of xenic cohabiting organisms. The effects of plant growth regulators were studied regarding new shoot formation, i.e. bud formation on the protonemal filaments and protonemal patch size. Low concentrations of cytokinin and medium concentrations of auxin are shown to increase protonemal patch size and shoot production. Multiplication of H. heimii was observed to occur spontaneously on BCD medium type, but to achieve better and rapid biomass production and development it is suggested to grow it on a BCD medium enriched with auxin and cytokinin combined.
{"title":"Micropropagation of rare bryo-halophyte Hennediella heimii","authors":"M. Ćosić, M. Sabovljević, B. Papp, Z. Giba, J. Šinžar-Sekulić, A. Sabovljevic, Milorad M Vujičić","doi":"10.2298/botserb2202187c","DOIUrl":"https://doi.org/10.2298/botserb2202187c","url":null,"abstract":"The rare moss species Hennediella heimii (Pottiaceae) was established in in vitro culture. Various treatments were tested to achieve axenical cultures. The most effective sterilising procedure was NaDCC treatment of sporophytes, keeping the spore viability and giving high disposal of xenic cohabiting organisms. The effects of plant growth regulators were studied regarding new shoot formation, i.e. bud formation on the protonemal filaments and protonemal patch size. Low concentrations of cytokinin and medium concentrations of auxin are shown to increase protonemal patch size and shoot production. Multiplication of H. heimii was observed to occur spontaneously on BCD medium type, but to achieve better and rapid biomass production and development it is suggested to grow it on a BCD medium enriched with auxin and cytokinin combined.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73519866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sennur Çalışkan, Pelin Gökfiliz-Yıldız, Ayşegül Özmen, H. Yıldırım, A. Uzel
This study aimed to investigate the endophytes of Juniperus macrocarpa collected from ?esme in ?zmir, Turkey, using a culture-dependent approach and to evaluate their antimicrobial activity for the first time. Since endophytes interact with phytochemicals of the host plant, in addition to the standard culture media, a J. macrocarpa extract supplemented culture media was also used for isolation to enhance the cultivability of the endophytes. Six bacteria out of twelve and three fungi out of seven were isolated from the plant extract supplemented culture media. The genotypic identification of the bacterial and fungal isolates was determined based on 16S rDNA and Internal Transcribed Spacer (ITS) sequence analysis, respectively. The genus Juniperus, which has ethnopharmacological uses, is rich in phytochemicals with multiple bioactivities. Since Juniperus spp. is listed as a priority natural habitat, it is necessary to find alternative resources that could replace the bioactive compounds of these plants. Endophytes of Juniperus spp. might be good candidates as antimicrobial producers. From this point of view, the antimicrobial activity of the crude fermentation liquid of the J. macrocarpa endophytes, and also aqueous and methanolic extracts of J. macrocarpa, were evaluated using a disc diffusion assay against a panel of test microorganisms, including antibiotic resistant ones. One fungus and seven bacteria showed remarkable antimicrobial activity against at least one test microorganism. These results indicated that some endophytes of J. macrocarpa had antimicrobial properties like their host plant and could substitute these plants as a source of antimicrobials.
{"title":"Juniperus macrocarpa endophytes isolated on standard‐ and plant extract supplemented-culture media - and evaluation of their antimicrobial activity","authors":"Sennur Çalışkan, Pelin Gökfiliz-Yıldız, Ayşegül Özmen, H. Yıldırım, A. Uzel","doi":"10.2298/botserb2202217c","DOIUrl":"https://doi.org/10.2298/botserb2202217c","url":null,"abstract":"This study aimed to investigate the endophytes of Juniperus macrocarpa collected from ?esme in ?zmir, Turkey, using a culture-dependent approach and to evaluate their antimicrobial activity for the first time. Since endophytes interact with phytochemicals of the host plant, in addition to the standard culture media, a J. macrocarpa extract supplemented culture media was also used for isolation to enhance the cultivability of the endophytes. Six bacteria out of twelve and three fungi out of seven were isolated from the plant extract supplemented culture media. The genotypic identification of the bacterial and fungal isolates was determined based on 16S rDNA and Internal Transcribed Spacer (ITS) sequence analysis, respectively. The genus Juniperus, which has ethnopharmacological uses, is rich in phytochemicals with multiple bioactivities. Since Juniperus spp. is listed as a priority natural habitat, it is necessary to find alternative resources that could replace the bioactive compounds of these plants. Endophytes of Juniperus spp. might be good candidates as antimicrobial producers. From this point of view, the antimicrobial activity of the crude fermentation liquid of the J. macrocarpa endophytes, and also aqueous and methanolic extracts of J. macrocarpa, were evaluated using a disc diffusion assay against a panel of test microorganisms, including antibiotic resistant ones. One fungus and seven bacteria showed remarkable antimicrobial activity against at least one test microorganism. These results indicated that some endophytes of J. macrocarpa had antimicrobial properties like their host plant and could substitute these plants as a source of antimicrobials.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"74 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76425656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Tomović, M. Sabovljević, I. Irimia, H. Taşkın, Eva Zupan, Petya Boycheva, Dobri Ivanov, B. Papp, J. Pantović, Aleksandra Marković, Sanja Djurovic, Uroš Buzurović, Sanja Sovran, E. Masic, S. Ștefănuț, T. Denchev, C. Denchev, Elvedin Šabanović, V. Djordjević, D. Stoykov, M. Niketić, M. Slavova, B. Assyov
This paper presents new records and noteworthy data on the following taxa in SE Europe and adjacent regions: red algae Lemanea rigida and Paralemanea torulosa, mycorrhizal fungi Amanita simulans and Terfezia pseudoleptoderma, parasitic fungus Microbotryum vinosum, saprotrophic fungus Sarcoscypha jurana, stonewort Chara tenuispina, mosses Brachytheciastrum collinum and Meesia longiseta, monocots Dactylorhiza romana and Neotinea maculata and dicots Adenophora liliifolia, Ambrosia artemisiifolia and Tanacetum corymbosum subsp. cinereum are given within SE Europe and adjacent regions.
{"title":"New records and noteworthy data of plants, algae and fungi in SE Europe and adjacent regions, 10","authors":"G. Tomović, M. Sabovljević, I. Irimia, H. Taşkın, Eva Zupan, Petya Boycheva, Dobri Ivanov, B. Papp, J. Pantović, Aleksandra Marković, Sanja Djurovic, Uroš Buzurović, Sanja Sovran, E. Masic, S. Ștefănuț, T. Denchev, C. Denchev, Elvedin Šabanović, V. Djordjević, D. Stoykov, M. Niketić, M. Slavova, B. Assyov","doi":"10.2298/botserb2202321t","DOIUrl":"https://doi.org/10.2298/botserb2202321t","url":null,"abstract":"This paper presents new records and noteworthy data on the following taxa in SE Europe and adjacent regions: red algae Lemanea rigida and Paralemanea torulosa, mycorrhizal fungi Amanita simulans and Terfezia pseudoleptoderma, parasitic fungus Microbotryum vinosum, saprotrophic fungus Sarcoscypha jurana, stonewort Chara tenuispina, mosses Brachytheciastrum collinum and Meesia longiseta, monocots Dactylorhiza romana and Neotinea maculata and dicots Adenophora liliifolia, Ambrosia artemisiifolia and Tanacetum corymbosum subsp. cinereum are given within SE Europe and adjacent regions.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"57 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78362954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Jakovljević, A. Bani, D. Pavlova, M. Konstantinou, P. Dimitrakopoulos, D. Kyrkas, R. Reeves, Tomica Mišljenović, G. Tomović, D. Van, A. Baker, Andonovska Baceva, J. Morel, G. Echevarria
Hyperaccumulator plants are able to tolerate extremely high concentrations of metals/metalloids in the soil in which they grow and to accumulate high concentrations in their shoots. To date, a total of 31 hyperaccumulator plant species have been identified in the Balkans, the centre of diversity and speciation in the European flora which is particularly rich in ultramafic areas. A further 8 species have yet to be confirmed through additional studies. Most of the 31 hyperaccumulator taxa (13 taxa or 41.9%) are species of the genus Odontarrhena, all hyperaccumulating Ni, but concentrations of this element above the hyperaccumulation threshold were also found in the genera Bornmuellera and Noccaea (all Brassicaceae), Orobanche (Orobanchaceae), Centaurea (Asteraceae) and Viola (Violaceae). The existence of hyperaccumulators of Tl and Zn is of particular interest because very few species worldwide hyperaccumulate these elements. Multiple metal hyperaccumulation was found in Noccaea kovatsii, as the hyperaccumulation of Zn was found in this species in addition to Ni, the primary accumulated element. Metal hyperaccumulation is discussed in terms of phylogenetic relationships and species distributions, with special attention to their systematics, the detection and recognition of new hyperaccumulating species and the possibilities for their future practical applications in phytotechnologies.
{"title":"Hyperaccumulator plant discoveries in the Balkans: Accumulation, distribution, and practical applications","authors":"K. Jakovljević, A. Bani, D. Pavlova, M. Konstantinou, P. Dimitrakopoulos, D. Kyrkas, R. Reeves, Tomica Mišljenović, G. Tomović, D. Van, A. Baker, Andonovska Baceva, J. Morel, G. Echevarria","doi":"10.2298/botserb2202161j","DOIUrl":"https://doi.org/10.2298/botserb2202161j","url":null,"abstract":"Hyperaccumulator plants are able to tolerate extremely high concentrations of metals/metalloids in the soil in which they grow and to accumulate high concentrations in their shoots. To date, a total of 31 hyperaccumulator plant species have been identified in the Balkans, the centre of diversity and speciation in the European flora which is particularly rich in ultramafic areas. A further 8 species have yet to be confirmed through additional studies. Most of the 31 hyperaccumulator taxa (13 taxa or 41.9%) are species of the genus Odontarrhena, all hyperaccumulating Ni, but concentrations of this element above the hyperaccumulation threshold were also found in the genera Bornmuellera and Noccaea (all Brassicaceae), Orobanche (Orobanchaceae), Centaurea (Asteraceae) and Viola (Violaceae). The existence of hyperaccumulators of Tl and Zn is of particular interest because very few species worldwide hyperaccumulate these elements. Multiple metal hyperaccumulation was found in Noccaea kovatsii, as the hyperaccumulation of Zn was found in this species in addition to Ni, the primary accumulated element. Metal hyperaccumulation is discussed in terms of phylogenetic relationships and species distributions, with special attention to their systematics, the detection and recognition of new hyperaccumulating species and the possibilities for their future practical applications in phytotechnologies.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"17 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90912947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Comparative palynological studies of Dipsacaceae s.str. show considerable diversity in the pollen grain morphology of its representatives. The aim of this research was to provide more detailed palynomorphological data on selected taxa whose pollen grains are characterised by tricolpate apertures (Pterocephalus plumosus, Succisa pratensis and Succisella inflexa). The pollen morphology of P. plumosus was studied using both light microscopy and scanning electron microscopy. The pollen grains are tricolpate, suboblate to prolate; large in size. Their outline in polar view (amb) is subcircular, rarely trilobate. The brevicolpi have a distinct margo and acute ends. The exine sculpture is echinate-microechinate-nanoechinate. The exine structure (columellae) of the pollen grains of P. plumosus, S. pratensis and S. inflexa was analysed for the first time in the current study. Pollen grains in P. plumosus are distinguished by long and dense columellae in the mesocolpia, and longer in the apocolpia. Our new ?alynomorphological data support a close relationship between S. pratensis and S. inflexa by the presence of shorter sparse columellae throughout the pollen exine. The UPGMA dendrogram shows the distinctive morphometric characters of P. plumosus pollen grains, which is thus included as a separate branch.
{"title":"Taxonomic significance of pollen morphology of Pterocephalus plumosus (Caprifoliaceae)","authors":"Z. Tsymbalyuk, Daniella Ivanova, L. Nitsenko","doi":"10.2298/botserb2202229t","DOIUrl":"https://doi.org/10.2298/botserb2202229t","url":null,"abstract":"Comparative palynological studies of Dipsacaceae s.str. show considerable diversity in the pollen grain morphology of its representatives. The aim of this research was to provide more detailed palynomorphological data on selected taxa whose pollen grains are characterised by tricolpate apertures (Pterocephalus plumosus, Succisa pratensis and Succisella inflexa). The pollen morphology of P. plumosus was studied using both light microscopy and scanning electron microscopy. The pollen grains are tricolpate, suboblate to prolate; large in size. Their outline in polar view (amb) is subcircular, rarely trilobate. The brevicolpi have a distinct margo and acute ends. The exine sculpture is echinate-microechinate-nanoechinate. The exine structure (columellae) of the pollen grains of P. plumosus, S. pratensis and S. inflexa was analysed for the first time in the current study. Pollen grains in P. plumosus are distinguished by long and dense columellae in the mesocolpia, and longer in the apocolpia. Our new ?alynomorphological data support a close relationship between S. pratensis and S. inflexa by the presence of shorter sparse columellae throughout the pollen exine. The UPGMA dendrogram shows the distinctive morphometric characters of P. plumosus pollen grains, which is thus included as a separate branch.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"10 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90382287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Kostić, D. Kitić, M. Vujović, Marija Marković, A. Pavlović, G. Stojanović
Headspace sampling is a fast, simple and economical way to prepare plant samples for analysis by gas chromatography. For the first time, the composition of the head space volatiles (HSV) of six Salvia species (S. verticillata, S. glutinosa, S. nemorosa, S. aethiopis, S. amplexicaulis and S. officinalis) in the flowering stage and two (S. glutinosa and S. sclarea) in the fruiting stage from Southeastern Serbia was analysed using the GC-FID-MS technique after headspace sampling. The chemical composition of the highly volatile compounds of the analysed species varies considerably. Monoterpene hydrocarbons represented the dominant class of volatile compounds in all the Salvia species, except for S. sclarea and S. aethiopis. The content of sesquiterpenes was the highest in S. aethiopis (96.9%) and S. glutinosa in the flowering phase (29.5%), while in all the other samples that percentage was below 10%. Oxygenated monoterpenes were the most abundant in S. sclarea, where the main component was oxygenated monoterpene linalyl acetate (97.7%). The main component of S. verticillata was ?-phellandrene, and its content varied depending on the plant location and sampling time. The main component of S. glutinosa in the flowering phase was limonene (16.6%), and in the fruiting phase sabinene (87.1%). Headspace analysis of the volatile components of S. aethiopis was carried out for the first time and the most abundant detected components were sesquiterpenes: (E)- caryophyllene (36.8%), ?-copaene (33.4%) and ?-elemene (7.3%). The analysis of the principal components was performed to interpret the grouping patterns, as well as to analyse the similarities and differences between the samples in terms of the composition of the volatile components. The samples were grouped into three clusters. The first cluster consisted of samples of S. verticillata (S1, S4 and S5) from different locations, the second comprised samples of S. glutinosa (S3), S. aethiopis (S8), S. amplexicaulis (S9) and S. officinalis (S10), while samples of S. nemorosa (S7) made up the third cluster. The HS-GC-FID-MS technique can be successfully used for the qualitative and quantitative analysis of volatile compounds of different Salvia species. The obtained results are important for evaluating the possibility of using different types of sage.
顶空取样是一种快速、简单、经济的制备植物样品的方法，用于气相色谱分析。采用气相色谱-红外光谱-质谱联用技术，首次对塞尔维亚东南部6种鼠尾草(S. verticillata, S. glutinosa, S. nemorosa, S. aethiopis, S. amplexicaulis和S. officinalis)开花期和2种鼠尾草(S. glutinosa和S. sclarea)的顶空挥发物(HSV)成分进行了分析。所分析物种的高挥发性化合物的化学成分差别很大。在所有鼠尾草品种中，单萜类化合物为挥发性化合物的优势类，除了ssclarea鼠尾草和saethiopis鼠尾草。倍半萜类化合物的含量以开花期的埃塞俄比亚葡萄(96.9%)和谷氨酸葡萄(29.5%)最高，其余样品均低于10%。其中含氧单萜含量最多，主要成分为芳樟酸单萜(97.7%)。黄花参的主要成分为?-香菜烯，其含量随植株位置和采样时间的不同而不同。开花期主要成分为柠檬烯(16.6%)，果期主要成分为沙宾烯(87.1%)。首次对埃塞俄比亚梭菌的挥发性成分进行顶空分析，丰度最高的是倍半萜类成分:(E)-石竹烯(36.8%)、-copaene(33.4%)和-榄香烯(7.3%)。主成分的分析是为了解释分组模式，以及分析样品之间在挥发性成分组成方面的相似性和差异性。这些样本被分成三组。第一个聚类由不同地点的轮状棘球绦虫(S1、S4和S5)组成，第二个聚类由谷氨酸棘球绦虫(S3)、埃塞俄比亚棘球绦虫(S8)、扩增棘球绦虫(S9)和officinalis (S10)组成，第三个聚类由线虫棘球绦虫(S7)组成。HS-GC-FID-MS技术可用于不同鼠尾草品种挥发性成分的定性和定量分析。所得结果对评价使用不同类型鼠尾草的可能性具有重要意义。
{"title":"A chemometric approach to the headspace sampled volatiles of selected Salvia species from Southeastern Serbia","authors":"E. Kostić, D. Kitić, M. Vujović, Marija Marković, A. Pavlović, G. Stojanović","doi":"10.2298/botserb2202285k","DOIUrl":"https://doi.org/10.2298/botserb2202285k","url":null,"abstract":"Headspace sampling is a fast, simple and economical way to prepare plant samples for analysis by gas chromatography. For the first time, the composition of the head space volatiles (HSV) of six Salvia species (S. verticillata, S. glutinosa, S. nemorosa, S. aethiopis, S. amplexicaulis and S. officinalis) in the flowering stage and two (S. glutinosa and S. sclarea) in the fruiting stage from Southeastern Serbia was analysed using the GC-FID-MS technique after headspace sampling. The chemical composition of the highly volatile compounds of the analysed species varies considerably. Monoterpene hydrocarbons represented the dominant class of volatile compounds in all the Salvia species, except for S. sclarea and S. aethiopis. The content of sesquiterpenes was the highest in S. aethiopis (96.9%) and S. glutinosa in the flowering phase (29.5%), while in all the other samples that percentage was below 10%. Oxygenated monoterpenes were the most abundant in S. sclarea, where the main component was oxygenated monoterpene linalyl acetate (97.7%). The main component of S. verticillata was ?-phellandrene, and its content varied depending on the plant location and sampling time. The main component of S. glutinosa in the flowering phase was limonene (16.6%), and in the fruiting phase sabinene (87.1%). Headspace analysis of the volatile components of S. aethiopis was carried out for the first time and the most abundant detected components were sesquiterpenes: (E)- caryophyllene (36.8%), ?-copaene (33.4%) and ?-elemene (7.3%). The analysis of the principal components was performed to interpret the grouping patterns, as well as to analyse the similarities and differences between the samples in terms of the composition of the volatile components. The samples were grouped into three clusters. The first cluster consisted of samples of S. verticillata (S1, S4 and S5) from different locations, the second comprised samples of S. glutinosa (S3), S. aethiopis (S8), S. amplexicaulis (S9) and S. officinalis (S10), while samples of S. nemorosa (S7) made up the third cluster. The HS-GC-FID-MS technique can be successfully used for the qualitative and quantitative analysis of volatile compounds of different Salvia species. The obtained results are important for evaluating the possibility of using different types of sage.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"218 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74938255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Slavkovska, B. Zlatković, M. Marčetić, N. Kovacevic, M. Drobac
This work was aimed at investigating the anatomy and composition of the essential oils from different organs of two Pimpinella species, P. tragium and P. saxifraga, originating from the central part of the Balkan Peninsula. The results of the analysis of the anatomy of the different organs of P. tragium and P. saxifraga showed that they were very similar in terms of the structure of the roots, stems and petioles. The roots showed a secondary structure and the stems had a primary structure with closed collateral vascular bundles. The petioles were cordatum, ribbed with an adaxial groove, and sparsely covered with short unior bicellular non-glandular trichomes with a pointed top (both species) or with long uniseriate, multicellular, non-glandular trichomes (P. saxifraga). Differences were observed in the anatomy of the leaves and in the indumentum of the fruits. The leaves of P. tragium were isobilateral, while those of P. saxifraga were dorsiventral. The P. tragium fruits were pubescent, covered with numerous, multicellular, uniseriate, straight or curved, gradually acuminate non-glandular trichomes, whereas the fruits of P. saxifraga were glabrous. These differences are of ecological and taxonomic significance. Secretory canals were observed in all the investigated organs of both species. The essential oils, isolated by hydrodistillation from the aerial parts with inflorescences, the roots from the flowering and fruiting period, and the fruits of both species, were analysed by GC-FID/MS. Although P. tragium and P. saxifraga essential oils showed certain differences in qualitative and quantitative patterns, all the oils were characterised by the presence of pseudoisoeugenol type phenylpropanoid epoxy-pseudoisoeugenyl-2-methylbutyrate, as well as by that of trinorsesquiterpenes pregeijerene, geijerene and trinoranastreptene. In addition, azulenes were present in all the root essential oils, whereas in the majority of the oils from the fruits and flowering aerial parts the common compound was the sesquiterpene ?-bisabolene. The occurrence of phenylpropanoids of pseudoisoeugenol type and geijerenes is important from the chemotaxonomic point of view and confirms them as chemical markers of the Pimpinella species analysed so far.
{"title":"The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae)","authors":"V. Slavkovska, B. Zlatković, M. Marčetić, N. Kovacevic, M. Drobac","doi":"10.2298/botserb2202269s","DOIUrl":"https://doi.org/10.2298/botserb2202269s","url":null,"abstract":"This work was aimed at investigating the anatomy and composition of the essential oils from different organs of two Pimpinella species, P. tragium and P. saxifraga, originating from the central part of the Balkan Peninsula. The results of the analysis of the anatomy of the different organs of P. tragium and P. saxifraga showed that they were very similar in terms of the structure of the roots, stems and petioles. The roots showed a secondary structure and the stems had a primary structure with closed collateral vascular bundles. The petioles were cordatum, ribbed with an adaxial groove, and sparsely covered with short unior bicellular non-glandular trichomes with a pointed top (both species) or with long uniseriate, multicellular, non-glandular trichomes (P. saxifraga). Differences were observed in the anatomy of the leaves and in the indumentum of the fruits. The leaves of P. tragium were isobilateral, while those of P. saxifraga were dorsiventral. The P. tragium fruits were pubescent, covered with numerous, multicellular, uniseriate, straight or curved, gradually acuminate non-glandular trichomes, whereas the fruits of P. saxifraga were glabrous. These differences are of ecological and taxonomic significance. Secretory canals were observed in all the investigated organs of both species. The essential oils, isolated by hydrodistillation from the aerial parts with inflorescences, the roots from the flowering and fruiting period, and the fruits of both species, were analysed by GC-FID/MS. Although P. tragium and P. saxifraga essential oils showed certain differences in qualitative and quantitative patterns, all the oils were characterised by the presence of pseudoisoeugenol type phenylpropanoid epoxy-pseudoisoeugenyl-2-methylbutyrate, as well as by that of trinorsesquiterpenes pregeijerene, geijerene and trinoranastreptene. In addition, azulenes were present in all the root essential oils, whereas in the majority of the oils from the fruits and flowering aerial parts the common compound was the sesquiterpene ?-bisabolene. The occurrence of phenylpropanoids of pseudoisoeugenol type and geijerenes is important from the chemotaxonomic point of view and confirms them as chemical markers of the Pimpinella species analysed so far.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"10 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76342602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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