Pub Date : 2023-02-03DOI: 10.1590/1678-4324-2023220352
L. Armstrong, F. J. Z. Merino, M. D. Miguel, A. I. L. Lordello, O. G. Miguel
: The chemical investigation of Eugenia pyriformis , popularly known as uvaia, led to the isolation of three compounds, the triterpene β -amiryn ( 1 ) and the porphyrin derivatives pheophytin a ( 2 ) and b ( 3 ). Their structures were assigned based on spectroscopic NMR analysis, as well as by comparison with literature data. Most of the compounds found in the essential oil from leaves are terpenes derivatives, such as ο - cymene; 1,8-cineol; linalol, terpinen-4-ol; 2-carene. Flavonoids, steroids and/or terpenoids, lactones, saponins, tannins and fixed acids were detected in both the leaf and stem crude extracts and fractions generated from the Brazilian E. pyriformis plant. According to the results of the DPPH assay, the ethyl acetate fraction of the leaf extract showed higher antioxidant activity than the other samples tested. In the phosphomolybdenum complex assay, the crude ethanol extract of the leaf and stem, and the ethyl acetate fraction of the leaf, showed a higher antioxidant capacity than rutin. It was observed that the chloroform and ethyl acetate fractions of the leaf, and the hexane, ethyl acetate and hydroalcoholic fractions of the stem were toxic to Artemia salina brine shrimp with LD 50 < 1.0 µg/mL. The hexane fraction of the stem was also allelopathic in inhibiting the radicle growth of Lactuca sativa seeds. These results adding contributions to chemical and biological studies of this medicinal species
{"title":"Chemical Profile of Essential Oil, Extracts and Fractions of Eugenia pyriformis Cambess. and its Antioxidant, Cytotoxic and Allelopathic Activities","authors":"L. Armstrong, F. J. Z. Merino, M. D. Miguel, A. I. L. Lordello, O. G. Miguel","doi":"10.1590/1678-4324-2023220352","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220352","url":null,"abstract":": The chemical investigation of Eugenia pyriformis , popularly known as uvaia, led to the isolation of three compounds, the triterpene β -amiryn ( 1 ) and the porphyrin derivatives pheophytin a ( 2 ) and b ( 3 ). Their structures were assigned based on spectroscopic NMR analysis, as well as by comparison with literature data. Most of the compounds found in the essential oil from leaves are terpenes derivatives, such as ο - cymene; 1,8-cineol; linalol, terpinen-4-ol; 2-carene. Flavonoids, steroids and/or terpenoids, lactones, saponins, tannins and fixed acids were detected in both the leaf and stem crude extracts and fractions generated from the Brazilian E. pyriformis plant. According to the results of the DPPH assay, the ethyl acetate fraction of the leaf extract showed higher antioxidant activity than the other samples tested. In the phosphomolybdenum complex assay, the crude ethanol extract of the leaf and stem, and the ethyl acetate fraction of the leaf, showed a higher antioxidant capacity than rutin. It was observed that the chloroform and ethyl acetate fractions of the leaf, and the hexane, ethyl acetate and hydroalcoholic fractions of the stem were toxic to Artemia salina brine shrimp with LD 50 < 1.0 µg/mL. The hexane fraction of the stem was also allelopathic in inhibiting the radicle growth of Lactuca sativa seeds. These results adding contributions to chemical and biological studies of this medicinal species","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67463027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.1590/1678-4324-2023210843
J. B. Schallemberger, Nelson Libardi, R. C. Puerari, W. G. Matias, Maria Eliza Nagel-Hassemer
: This study evaluated the removal mechanisms of azo dyes from synthetic textile wastewater using spent mushroom substrate (SMS). The simultaneous adsorption and enzymatic degradation were analyzed. The effect of pH, SMS concentration and temperature were studied. The maximum enzymatic degradation (16.36%) was obtained at pH 4 and 65 g SMS L -1 , whereas the maximum adsorption (62.86%) at pH 8 and 155 g SMS L -1 , both at 25°C. The simultaneous enzymatic degradation and adsorption led to the removal of 75.67%. with increased color change. The kinetic study showed that dye adsorption was obtained in the first 30 minutes while the enzymatic degradation increased slowly over the time. The treatment with SMS resulted in the removal of 28.75% of the true color and no increase of effluent toxicity.
{"title":"Effect of Spent Mushroom Substrate on Azo Dye Removal and Effluent Treatment","authors":"J. B. Schallemberger, Nelson Libardi, R. C. Puerari, W. G. Matias, Maria Eliza Nagel-Hassemer","doi":"10.1590/1678-4324-2023210843","DOIUrl":"https://doi.org/10.1590/1678-4324-2023210843","url":null,"abstract":": This study evaluated the removal mechanisms of azo dyes from synthetic textile wastewater using spent mushroom substrate (SMS). The simultaneous adsorption and enzymatic degradation were analyzed. The effect of pH, SMS concentration and temperature were studied. The maximum enzymatic degradation (16.36%) was obtained at pH 4 and 65 g SMS L -1 , whereas the maximum adsorption (62.86%) at pH 8 and 155 g SMS L -1 , both at 25°C. The simultaneous enzymatic degradation and adsorption led to the removal of 75.67%. with increased color change. The kinetic study showed that dye adsorption was obtained in the first 30 minutes while the enzymatic degradation increased slowly over the time. The treatment with SMS resulted in the removal of 28.75% of the true color and no increase of effluent toxicity.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67460154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: Umbilical cord mesenchymal stem cells (UMSCs) from fetal tissues have become a valuable source of cell regeneration therapy due to their low immunogenicity, high plasticity and antimicrobial peptides. Most researches about UMSCs mainly focused on humans, mice, rabbits and other mammals
{"title":"In Vitro Culture and Multipotency Evaluation of Broiler Umbilical Cord Mesenchymal Stem Cells","authors":"Caiyun Ma, Gaofeng Liu, Chunjing Wang, Yu Guo, W. Guan, Changqing Liu, Hebao Wen","doi":"10.1590/1678-4324-2023220031","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220031","url":null,"abstract":": Umbilical cord mesenchymal stem cells (UMSCs) from fetal tissues have become a valuable source of cell regeneration therapy due to their low immunogenicity, high plasticity and antimicrobial peptides. Most researches about UMSCs mainly focused on humans, mice, rabbits and other mammals","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67460400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.1590/1678-4324-2023220166
Milind Dattatraya Giri, N. Patke, Y. Ingle
: Intensive growth of the pigeonpea crop in Central and South India, caused by adequate rainfall, resulted in taller plants with fewer fruited branches, leading to lower yields. However, pigeonpea crops could produce more pods if they restricted apical growth. Nipping (Cutting off the top growing portion) and planting distances are effective techniques to control apical dominance. Thus, there is a need to explore the benefits of simple techniques like nipping and planting distance, suppressing apical dominance. Therefore, this study aimed to determine the best nipping time and planting distance for pigeonpea in a rainfed environment to increase productivity. The experiment was conducted at the Dr. Panjabrao Deshmukh Krishi Vidyapeeth (Agricultural University), Akola (Maharashtra), India, between 2018 and 2020. The experiment was laid out in a randomized complete block design with two factors with three replications. There were three timings of nipping (45 days after planting, 60 days after planting, and no nipping) and four planting distances (90cm x 30cm, 120cm x 30cm, 150cm x 30cm, and 180cm x 30cm). Results indicated that early loss of apical dominance due to the nipping at 45 days after planting resulted in reduced plant height, increased branches, dry-matter accumulation, pods per plant, and markedly increased pigeon pea productivity by 11 percent. In addition, Pigeonpea crops' growth and yield parameters improved when planted at 180cm x 30cm. However, growing pigeonpea at a distance of 90cm x 30cm resulted in significantly higher pigeonpea seed yield, harvest index, and rainwater use efficiency than planting at a wider spacing.
{"title":"Pigeonpea Apical Dominance is Controlled for Optimum Yield via Nipping and Planting Distance","authors":"Milind Dattatraya Giri, N. Patke, Y. Ingle","doi":"10.1590/1678-4324-2023220166","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220166","url":null,"abstract":": Intensive growth of the pigeonpea crop in Central and South India, caused by adequate rainfall, resulted in taller plants with fewer fruited branches, leading to lower yields. However, pigeonpea crops could produce more pods if they restricted apical growth. Nipping (Cutting off the top growing portion) and planting distances are effective techniques to control apical dominance. Thus, there is a need to explore the benefits of simple techniques like nipping and planting distance, suppressing apical dominance. Therefore, this study aimed to determine the best nipping time and planting distance for pigeonpea in a rainfed environment to increase productivity. The experiment was conducted at the Dr. Panjabrao Deshmukh Krishi Vidyapeeth (Agricultural University), Akola (Maharashtra), India, between 2018 and 2020. The experiment was laid out in a randomized complete block design with two factors with three replications. There were three timings of nipping (45 days after planting, 60 days after planting, and no nipping) and four planting distances (90cm x 30cm, 120cm x 30cm, 150cm x 30cm, and 180cm x 30cm). Results indicated that early loss of apical dominance due to the nipping at 45 days after planting resulted in reduced plant height, increased branches, dry-matter accumulation, pods per plant, and markedly increased pigeon pea productivity by 11 percent. In addition, Pigeonpea crops' growth and yield parameters improved when planted at 180cm x 30cm. However, growing pigeonpea at a distance of 90cm x 30cm resulted in significantly higher pigeonpea seed yield, harvest index, and rainwater use efficiency than planting at a wider spacing.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67461200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.1590/1678-4324-2023220314
Valdenir Pereira Morais, Fabisleine Vieira Cabral, C. Fernandes, M. Miranda
: This literature brief review compiled the main biological activities and active chemical constituents that have already been isolated from Piper aduncum (Piperaceae), whose common name is spiked paper. In Brazilian Portuguese, it has been called pimenta-longa, aperta-ruão, pimenta de macaco, matico, erva de jaboti, jaborandi do mato, pimenta - de-fruto-ganchoso and tapa buraco . Extracts, essential oils (EOs) and compounds isolated from this species have exhibited remarkable fungicidal and insecticidal activities, besides antibacterial, antileishmanial, antioxidant, cytotoxic/antitumor, larvicidal, antiplatelet, molluscoidal and antiviral ones. P. aduncum is an endemic species in Brazil which has drawn researchers’ attention because of high contents of EOs that are extracted from its leaves, inflorescences and twigs. This study aimed at analyzing data on P. aduncum , its EOs, extracts and isolated compounds found in 45 research papers. Its major chemical constituent is definitely dillapiole, an arylpropanoid. Findings were carefully compiled by this brief review which reinforces the chemical and biological potential of this botanic species.
{"title":"Brief Review on Piper aduncum L., its Bioactive Metabolites and its Potential to Develop Bioproducts","authors":"Valdenir Pereira Morais, Fabisleine Vieira Cabral, C. Fernandes, M. Miranda","doi":"10.1590/1678-4324-2023220314","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220314","url":null,"abstract":": This literature brief review compiled the main biological activities and active chemical constituents that have already been isolated from Piper aduncum (Piperaceae), whose common name is spiked paper. In Brazilian Portuguese, it has been called pimenta-longa, aperta-ruão, pimenta de macaco, matico, erva de jaboti, jaborandi do mato, pimenta - de-fruto-ganchoso and tapa buraco . Extracts, essential oils (EOs) and compounds isolated from this species have exhibited remarkable fungicidal and insecticidal activities, besides antibacterial, antileishmanial, antioxidant, cytotoxic/antitumor, larvicidal, antiplatelet, molluscoidal and antiviral ones. P. aduncum is an endemic species in Brazil which has drawn researchers’ attention because of high contents of EOs that are extracted from its leaves, inflorescences and twigs. This study aimed at analyzing data on P. aduncum , its EOs, extracts and isolated compounds found in 45 research papers. Its major chemical constituent is definitely dillapiole, an arylpropanoid. Findings were carefully compiled by this brief review which reinforces the chemical and biological potential of this botanic species.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67462742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.1590/1678-4324-2023210442
Mariana Maciel Cavalcanti, T. L. Sampaio, D. B. Lima, Marcus Felipe Bezerra da Costa, I. E. P. Azevedo, M. L. Monteiro, J. Evangelista, M. Bandeira, A. Martins
: Ischemia/reperfusion (I/R) -induced Acute kidney injury (AKI) is characterized by hypoxia and production of reactive oxygen species (ROS), which could be prevented with antioxidant agents. The essential oil of Lippia alba (EOLA) chemotype citral-limonene is rich in components with antioxidant activity. So, this study aims to evaluate the nephroprotective effect of the EOLA on in vivo and in vitro models of renal I/R. Male Wistar rats were submitted to right nephrectomy, followed by ischemia by clamping the renal artery in the left kidney and a reperfusion. Animals received EOLA (200 mg/kg) or vehicle three days prior to I/R surgery. Blood and urine samples were obtained to evaluate creatinine, urea, uric acid, and creatinine clearance. The left kidney was collected for histological evaluations and analysis of thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH). HK-2 cells were used to evaluate the effect of EOLA on I/R in vitro
{"title":"Essential Oil of Lippia alba Protects Against Ischemic-Reperfusion Acute Kidney Injury","authors":"Mariana Maciel Cavalcanti, T. L. Sampaio, D. B. Lima, Marcus Felipe Bezerra da Costa, I. E. P. Azevedo, M. L. Monteiro, J. Evangelista, M. Bandeira, A. Martins","doi":"10.1590/1678-4324-2023210442","DOIUrl":"https://doi.org/10.1590/1678-4324-2023210442","url":null,"abstract":": Ischemia/reperfusion (I/R) -induced Acute kidney injury (AKI) is characterized by hypoxia and production of reactive oxygen species (ROS), which could be prevented with antioxidant agents. The essential oil of Lippia alba (EOLA) chemotype citral-limonene is rich in components with antioxidant activity. So, this study aims to evaluate the nephroprotective effect of the EOLA on in vivo and in vitro models of renal I/R. Male Wistar rats were submitted to right nephrectomy, followed by ischemia by clamping the renal artery in the left kidney and a reperfusion. Animals received EOLA (200 mg/kg) or vehicle three days prior to I/R surgery. Blood and urine samples were obtained to evaluate creatinine, urea, uric acid, and creatinine clearance. The left kidney was collected for histological evaluations and analysis of thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH). HK-2 cells were used to evaluate the effect of EOLA on I/R in vitro","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"107 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67460064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.1590/1678-4324-2023210792
José Manuel Ramírez-Ochoa, Sofia Michelle Moreno-Fernández, J. M. Juárez-Barrientos, Juan Pablo Alcántar-Vázquez, Nicolás Valenzuela-Jiménez, Raúl Moreno-de la Torre
: One of the main problems to be solved in the commercial production of Nile tilapia is the use of large volumes of steroids during the sex reversion process. The precise evaluation of the time after hatching to start the steroid treatment could help reduce its use in the short-term. The main objective of this work was to evaluate the sex proportion and the growth obtained in a mixed population of Nile tilapia fed fluoxymesterone at different days (3, 6, 9, 12, and 15) after hatching. A one-factor experiment was designed, with the day, after hatching, that the fluoxymesterone (5 mg) began to be supplied as factor (3, 6, 9, 12 and 15). Initial stocking density was 0.4 larvae L -1 . All groups were evaluated by triplicate. Steroid treatment finished at day 25 after larvae stocking. Squash technique was used to determine sex proportion at the end of the experiment (45 days). Growth evaluation was performed based on wet weight and total length obtained from each biometry. The results showed the highest percentage of males (92%) in the fish fed fluoxymesterone from day three after hatching. No anabolic effect on growth was observed in any steroid-treated fish. A negative effect on growth was observed after withdrawal of the steroid in the fish that were fed fluoxymesterone for the longest time. These results show that it is necessary to supply the fluoxymesterone prior to the onset of sex differentiation (5-6 days after hatching) to achieve a permanent reversion of the sex differentiation process.
{"title":"Effect of Fluoxymesterone on Sex Proportion and Growth Performance of Nile Tilapia (Oreochromis niloticus L.).","authors":"José Manuel Ramírez-Ochoa, Sofia Michelle Moreno-Fernández, J. M. Juárez-Barrientos, Juan Pablo Alcántar-Vázquez, Nicolás Valenzuela-Jiménez, Raúl Moreno-de la Torre","doi":"10.1590/1678-4324-2023210792","DOIUrl":"https://doi.org/10.1590/1678-4324-2023210792","url":null,"abstract":": One of the main problems to be solved in the commercial production of Nile tilapia is the use of large volumes of steroids during the sex reversion process. The precise evaluation of the time after hatching to start the steroid treatment could help reduce its use in the short-term. The main objective of this work was to evaluate the sex proportion and the growth obtained in a mixed population of Nile tilapia fed fluoxymesterone at different days (3, 6, 9, 12, and 15) after hatching. A one-factor experiment was designed, with the day, after hatching, that the fluoxymesterone (5 mg) began to be supplied as factor (3, 6, 9, 12 and 15). Initial stocking density was 0.4 larvae L -1 . All groups were evaluated by triplicate. Steroid treatment finished at day 25 after larvae stocking. Squash technique was used to determine sex proportion at the end of the experiment (45 days). Growth evaluation was performed based on wet weight and total length obtained from each biometry. The results showed the highest percentage of males (92%) in the fish fed fluoxymesterone from day three after hatching. No anabolic effect on growth was observed in any steroid-treated fish. A negative effect on growth was observed after withdrawal of the steroid in the fish that were fed fluoxymesterone for the longest time. These results show that it is necessary to supply the fluoxymesterone prior to the onset of sex differentiation (5-6 days after hatching) to achieve a permanent reversion of the sex differentiation process.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67460186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.1590/1678-4324-2023210821
Aylin Gazdağlı, Gizem Yıldırım Baştemur, E. Yörük, S. Özkorucuklu, G. Albayrak
: Fusarium culmorum produces polyketide-structured aurofusarin and its precursor metabolites. Aurofusarin biosynthetic pathway is catalysed by the products of eleven genes located in gene cluster. In this study, the effects of the expression levels of Pks12 , Gip6 , and
{"title":"Molecular Analysis of the Genes Responsible for Catalysing Intracellular Steps of Aurofusarin Biosynthesis in Fusarium culmorum","authors":"Aylin Gazdağlı, Gizem Yıldırım Baştemur, E. Yörük, S. Özkorucuklu, G. Albayrak","doi":"10.1590/1678-4324-2023210821","DOIUrl":"https://doi.org/10.1590/1678-4324-2023210821","url":null,"abstract":": Fusarium culmorum produces polyketide-structured aurofusarin and its precursor metabolites. Aurofusarin biosynthetic pathway is catalysed by the products of eleven genes located in gene cluster. In this study, the effects of the expression levels of Pks12 , Gip6 , and","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"68 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67460250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.1590/1678-4324-2023220644
Lucas Braga Albino, R. Sordi, J. G. Oliveira, Daniel Fernandes
: Adenosine is an important regulator within the cardiovascular system and modulates various processes through four distinct G protein-coupled receptors (A 1 , A 2A , A 2B , and A 3 ), causing hypotension and reduced renal blood flow, which contributes to ischemia tissue and organ dysfunction. Caffeine causes most of its biological effects by blocking adenosine receptors. Although caffeine is vastly used as a pharmacological tool in basic research, there is a lack of studies characterizing the caffeine doses necessary for blocking the cardiovascular effects of adenosine. Therefore, we evaluated the effect of different doses of caffeine (10, 30 and 100 mg/kg, s.c) on the cardiovascular changes induced by adenosine. In addition, the adenosine response was evaluated at different times (4 and 8 hours) after caffeine administration. The bolus injection of increasing doses of adenosine dose-dependently reduced blood pressure, renal blood flow and heart rate. The dose of 30 mg/kg was the most effective in blocking adenosine-induced cardiovascular events. However, the higher dose of caffeine (100 mg/kg) fails to block the adenosine effect on heart rate. The effect of caffeine lasts for 4 hours. Therefore, caffeine in the dose of 30 mg/kg can block the effects of adenosine for up to 4 hours. These data provide valuable information on the dose and frequency of caffeine administration for future studies in rats.
{"title":"Dose and Time-Dependent Effects of Caffeine on Cardiovascular Changes Induced by Adenosine","authors":"Lucas Braga Albino, R. Sordi, J. G. Oliveira, Daniel Fernandes","doi":"10.1590/1678-4324-2023220644","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220644","url":null,"abstract":": Adenosine is an important regulator within the cardiovascular system and modulates various processes through four distinct G protein-coupled receptors (A 1 , A 2A , A 2B , and A 3 ), causing hypotension and reduced renal blood flow, which contributes to ischemia tissue and organ dysfunction. Caffeine causes most of its biological effects by blocking adenosine receptors. Although caffeine is vastly used as a pharmacological tool in basic research, there is a lack of studies characterizing the caffeine doses necessary for blocking the cardiovascular effects of adenosine. Therefore, we evaluated the effect of different doses of caffeine (10, 30 and 100 mg/kg, s.c) on the cardiovascular changes induced by adenosine. In addition, the adenosine response was evaluated at different times (4 and 8 hours) after caffeine administration. The bolus injection of increasing doses of adenosine dose-dependently reduced blood pressure, renal blood flow and heart rate. The dose of 30 mg/kg was the most effective in blocking adenosine-induced cardiovascular events. However, the higher dose of caffeine (100 mg/kg) fails to block the adenosine effect on heart rate. The effect of caffeine lasts for 4 hours. Therefore, caffeine in the dose of 30 mg/kg can block the effects of adenosine for up to 4 hours. These data provide valuable information on the dose and frequency of caffeine administration for future studies in rats.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67463676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.1590/1678-4324-2023220180
N. Şahin, N. Bilgiçli, A. Sayaslan
: Red dog flour (RDF), a milling by-product, is rich in nutritionally and functionally crucial components. Extrusion conditions for extruded corn snacks (ECS) with RDF were optimized using Response Surface Methodology (RSM) with Box-Behnken Design (moisture content: 13.5%, RDF ratio: 20%, screw speed: 468 rpm, and die temperature: 110°C). Then, functional, nutritional, and sensory properties of ECS containing an increasing ratio of RDF were determined. The addition of RDF improved ash (1,84 fold), fat (2,7 fold) protein (0,51 fold), dietary fiber (roughly 2,8 fold), and mineral content (Fe, K Mg, P, and Se) significantly compared to ECS without RDF. Extrusion condition considerably declined the total phenolic content (from 144.50 to 25,09 mg GAE/100g), Trolox equivalent of antioxidant capacity (from 1.13 to 0.41 mMol Trolox/g), and phytic acid content (from 2147 to 968.11 mg/100g) of ECS with RDF compared to raw materials. However, the increasing ratio of RDF had an increment in bioactive compounds (up to 2,38 fold mg GAE/100g, and 1,63 fold mMol Trolox/g) and phytic acid content (up to 0.36 fold). According to the sensory evaluation, 30% of ECS with RDF was the most preferable snack.
{"title":"Improvement of Functional and Nutritional Properties of Extruded Snacks with the Utilization of Red Dog Flour","authors":"N. Şahin, N. Bilgiçli, A. Sayaslan","doi":"10.1590/1678-4324-2023220180","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220180","url":null,"abstract":": Red dog flour (RDF), a milling by-product, is rich in nutritionally and functionally crucial components. Extrusion conditions for extruded corn snacks (ECS) with RDF were optimized using Response Surface Methodology (RSM) with Box-Behnken Design (moisture content: 13.5%, RDF ratio: 20%, screw speed: 468 rpm, and die temperature: 110°C). Then, functional, nutritional, and sensory properties of ECS containing an increasing ratio of RDF were determined. The addition of RDF improved ash (1,84 fold), fat (2,7 fold) protein (0,51 fold), dietary fiber (roughly 2,8 fold), and mineral content (Fe, K Mg, P, and Se) significantly compared to ECS without RDF. Extrusion condition considerably declined the total phenolic content (from 144.50 to 25,09 mg GAE/100g), Trolox equivalent of antioxidant capacity (from 1.13 to 0.41 mMol Trolox/g), and phytic acid content (from 2147 to 968.11 mg/100g) of ECS with RDF compared to raw materials. However, the increasing ratio of RDF had an increment in bioactive compounds (up to 2,38 fold mg GAE/100g, and 1,63 fold mMol Trolox/g) and phytic acid content (up to 0.36 fold). According to the sensory evaluation, 30% of ECS with RDF was the most preferable snack.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67461351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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