Pub Date : 2023-04-14DOI: 10.1590/1678-4324-2023220053
Fatma Ozkan, Z. Canturk
: Selonsertib (GS-4997), a serine/threonine kinase inhibitor, targets apoptosis signal-regulating kinase 1 (ASK1) and is now in a phase III clinical trial for the treatment of non-alcoholic steatohepatitis (NASH). Inflammation is a defense against an effect that can damage the tissue or organ itself. Cytokines are the agents that play a key role in acute inflammation and one of the most important cytokine proteins, IL-1β. In this study, we investigated whether selonsertib and diclofenac sodium (positive control) could inhibit IL-1β caused by two different LPS ( Salmonella enterica serotype enteritidis and Escherichia coli O111:B4) stimulated on THP-1 cell line. As a result of the WST-1 assay, the inhibitory concentration of diclofenac sodium at doses of IC 50 of 32.5 μM/mL and selonsertib was calculated to be 120 μM/mL. The immunological responses of diclofenac sodium and selonsertib (IL-1β) in different LPS-stimulated THP-1 cells were assessed cytometrically: diclofenac sodium and Selonsertib were found to be effective in stimulation from E. coli . The results showed that selonsertib had a significantly anti-inflammatory effect like diclofenac sodium.
{"title":"The Anti-inflammatory Effect of Selonsertib (GS-4997), an ASK1 Inhibitor, on LPS-Stimulated THP-1 Cells","authors":"Fatma Ozkan, Z. Canturk","doi":"10.1590/1678-4324-2023220053","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220053","url":null,"abstract":": Selonsertib (GS-4997), a serine/threonine kinase inhibitor, targets apoptosis signal-regulating kinase 1 (ASK1) and is now in a phase III clinical trial for the treatment of non-alcoholic steatohepatitis (NASH). Inflammation is a defense against an effect that can damage the tissue or organ itself. Cytokines are the agents that play a key role in acute inflammation and one of the most important cytokine proteins, IL-1β. In this study, we investigated whether selonsertib and diclofenac sodium (positive control) could inhibit IL-1β caused by two different LPS ( Salmonella enterica serotype enteritidis and Escherichia coli O111:B4) stimulated on THP-1 cell line. As a result of the WST-1 assay, the inhibitory concentration of diclofenac sodium at doses of IC 50 of 32.5 μM/mL and selonsertib was calculated to be 120 μM/mL. The immunological responses of diclofenac sodium and selonsertib (IL-1β) in different LPS-stimulated THP-1 cells were assessed cytometrically: diclofenac sodium and Selonsertib were found to be effective in stimulation from E. coli . The results showed that selonsertib had a significantly anti-inflammatory effect like diclofenac sodium.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67460939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-14DOI: 10.1590/1678-4324-2023220101
Murali Duraisamy
: Recently, the research is towards the development of high static gain DC-DC converters suitable for renewable energy applications. The high voltage gain can be achieved using isolated and non-isolated configurations of the converter. The magnetically coupled inductor based isolated DC-DC converter structures can have improved voltage gain, but they need large size inductors which may lead to increased cost. Thus, the modified structures of non-isolated conventional boost, CUK, and SEPIC topologies with inclusion of additional controlled and uncontrolled switches along with large number of passive components are employed to achieve the improved voltage gain. However, it leads to increased complexity and control. Hence, the researchers concentrated on development of hybrid non-isolated DC-DC converter topologies capable of achieving enhanced static voltage gain, without adding extra controlled switches and passive elements. In this paper, a hybrid non-isolated single-switch DC-DC converter structure is proposed for achieving high voltage gain than that of traditional non-isolated topologies. The proposed hybrid structure operating in continuous inductor current mode is obtained by connecting the conventional boost and the modified CUK converters in parallel. The power switch and the diodes have low voltage-current stress. The operation of the proposed hybrid topology during various modes is explored. The mathematical modeling of the proposed converter is also provided. The MATLAB / SIMULINK study of the suggested hybrid converter has been implemented. The digital simulation study proves the feasibility of the proposed hybrid converter concept and its steady-state behavior.
{"title":"A Transformerless Boost-Modified Cuk Combined Single-Switch DC-DC Converter Topology with Enhanced Voltage Gain","authors":"Murali Duraisamy","doi":"10.1590/1678-4324-2023220101","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220101","url":null,"abstract":": Recently, the research is towards the development of high static gain DC-DC converters suitable for renewable energy applications. The high voltage gain can be achieved using isolated and non-isolated configurations of the converter. The magnetically coupled inductor based isolated DC-DC converter structures can have improved voltage gain, but they need large size inductors which may lead to increased cost. Thus, the modified structures of non-isolated conventional boost, CUK, and SEPIC topologies with inclusion of additional controlled and uncontrolled switches along with large number of passive components are employed to achieve the improved voltage gain. However, it leads to increased complexity and control. Hence, the researchers concentrated on development of hybrid non-isolated DC-DC converter topologies capable of achieving enhanced static voltage gain, without adding extra controlled switches and passive elements. In this paper, a hybrid non-isolated single-switch DC-DC converter structure is proposed for achieving high voltage gain than that of traditional non-isolated topologies. The proposed hybrid structure operating in continuous inductor current mode is obtained by connecting the conventional boost and the modified CUK converters in parallel. The power switch and the diodes have low voltage-current stress. The operation of the proposed hybrid topology during various modes is explored. The mathematical modeling of the proposed converter is also provided. The MATLAB / SIMULINK study of the suggested hybrid converter has been implemented. The digital simulation study proves the feasibility of the proposed hybrid converter concept and its steady-state behavior.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67461016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-03DOI: 10.1590/1678-4324-2023220324
Marcello Rocha de Brito Júnior, Fábio Zacouteguy Ugalde, L. V. Gonzaga, Mayara Schulz, R. Fett, A. C. O. Costa, G. Tribuzi
: Juçara fruit ( Euterpe edulis Martius) is rich in nutrients and antioxidant compounds, a potential ingredient to meet consumer demands for beers with bioactive potential. In this study, different concentrations (10, 20, and 40
{"title":"Physicochemical Characteristics and Antioxidant Potential of a Fruit Beer Produced with Juçara (Euterpe edulis Martius) Fruit Pulp","authors":"Marcello Rocha de Brito Júnior, Fábio Zacouteguy Ugalde, L. V. Gonzaga, Mayara Schulz, R. Fett, A. C. O. Costa, G. Tribuzi","doi":"10.1590/1678-4324-2023220324","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220324","url":null,"abstract":": Juçara fruit ( Euterpe edulis Martius) is rich in nutrients and antioxidant compounds, a potential ingredient to meet consumer demands for beers with bioactive potential. In this study, different concentrations (10, 20, and 40","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67462898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-13DOI: 10.1590/1678-4324-2023220442
Thiago Luz, C. Vila, A. R. Aoki
: The benefits of complementarity between renewable energy sources have been presented as having a positive impact, but it has been neglected in generation expansion planning studies. In this paper the complementarity between renewable energy sources and regions of Brazil is characterized and the correlation coefficients are analyzed. A methodology is also proposed to optimize the complementarity between the sources, reducing the energy storage requirements. The methodology is applied to the Brazilian case to meet the projected demand for 2050 with a 100% renewable system and the complementarity is analyzed. The characterization of the data allowed us to propose five solar regions, four wind regions, three hydro regions and a region to represent the biomass. It was possible to find complementarity (negative correlation) of: 0.97 between hydro regions; 0.86 between wind and hydro regions and; 0.96 between biomass and hydro. The proposed methodology allowed us to know which regions and their respective technologies best benefit the electrical system, being the North for solar and the Northeast for wind. Finally, it was possible to reduce the demand for water storage with sources and regions that complement the seasonal hydro drought period.
{"title":"Complementarity Between Renewable Energy Sources and Regions - Brazilian Case","authors":"Thiago Luz, C. Vila, A. R. Aoki","doi":"10.1590/1678-4324-2023220442","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220442","url":null,"abstract":": The benefits of complementarity between renewable energy sources have been presented as having a positive impact, but it has been neglected in generation expansion planning studies. In this paper the complementarity between renewable energy sources and regions of Brazil is characterized and the correlation coefficients are analyzed. A methodology is also proposed to optimize the complementarity between the sources, reducing the energy storage requirements. The methodology is applied to the Brazilian case to meet the projected demand for 2050 with a 100% renewable system and the complementarity is analyzed. The characterization of the data allowed us to propose five solar regions, four wind regions, three hydro regions and a region to represent the biomass. It was possible to find complementarity (negative correlation) of: 0.97 between hydro regions; 0.86 between wind and hydro regions and; 0.96 between biomass and hydro. The proposed methodology allowed us to know which regions and their respective technologies best benefit the electrical system, being the North for solar and the Northeast for wind. Finally, it was possible to reduce the demand for water storage with sources and regions that complement the seasonal hydro drought period.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67463231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-13DOI: 10.1590/1678-4324-2023220316
Bihter Das, S. Toraman
: The new coronavirus SARS-CoV-2 is an infectious virus with a long incubation period, which was first detected in Wuhan, China, spread all over the world, seriously threatening human life. Therefore, accurate and rapid detection of SARS-CoV-2 is very important for controlling the epidemic and preventing its further spread. Currently, nucleic acid detection makes an important contribution to the prevention and control of SARS-CoV-2. In this study, a new and highly sensitive nucleic acid detection method for SARS-CoV-2 has been proposed. The nucleic acid sequences were digitized by Entropy-based mapping technique. Then, the digitized these sequences were divided into 100-unit sections using the sliding window method and given as input to Capsule Networks.10988 segments (5494 SARS-CoV-2, 5494 normal) are classified with capsule nets. With the proposed method, an accuracy performance of 100% was achieved by using capsule networks to identify SARS-CoV-2 from nucleic acid sequences. The results show that the proposed method successfully identifies SARS-CoV-2 from nucleic acid sequences.
{"title":"New Coronavirus 2 (SARS-CoV-2) Detection Method from Human Nucleic Acid Sequences Using Capsule Networks","authors":"Bihter Das, S. Toraman","doi":"10.1590/1678-4324-2023220316","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220316","url":null,"abstract":": The new coronavirus SARS-CoV-2 is an infectious virus with a long incubation period, which was first detected in Wuhan, China, spread all over the world, seriously threatening human life. Therefore, accurate and rapid detection of SARS-CoV-2 is very important for controlling the epidemic and preventing its further spread. Currently, nucleic acid detection makes an important contribution to the prevention and control of SARS-CoV-2. In this study, a new and highly sensitive nucleic acid detection method for SARS-CoV-2 has been proposed. The nucleic acid sequences were digitized by Entropy-based mapping technique. Then, the digitized these sequences were divided into 100-unit sections using the sliding window method and given as input to Capsule Networks.10988 segments (5494 SARS-CoV-2, 5494 normal) are classified with capsule nets. With the proposed method, an accuracy performance of 100% was achieved by using capsule networks to identify SARS-CoV-2 from nucleic acid sequences. The results show that the proposed method successfully identifies SARS-CoV-2 from nucleic acid sequences.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67462829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-13DOI: 10.1590/1678-4324-2023220278
C. Pezzini, Kássia Cristina Freire Zilch, S. M. Jahnke, A. Köhler
: During the storage period of dry tobacco and its derivatives, insect pests such as species of Ephestia Guenée (Lepidoptera: Pyralidae), popularly known as moths, cause damage to the product, being controlled mainly with physical practices and synthetic chemicals, although with limitations on their use and results. Some biological control agents, such as the parasitoid Habrobracon hebetor (Say) (Hymenoptera
{"title":"Effect of Habrobracon hebetor (Hymenoptera: Braconidae) Release on Moth Infestation in Stored Tobacco","authors":"C. Pezzini, Kássia Cristina Freire Zilch, S. M. Jahnke, A. Köhler","doi":"10.1590/1678-4324-2023220278","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220278","url":null,"abstract":": During the storage period of dry tobacco and its derivatives, insect pests such as species of Ephestia Guenée (Lepidoptera: Pyralidae), popularly known as moths, cause damage to the product, being controlled mainly with physical practices and synthetic chemicals, although with limitations on their use and results. Some biological control agents, such as the parasitoid Habrobracon hebetor (Say) (Hymenoptera","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67462440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-13DOI: 10.1590/1678-4324-2023220279
Moises Silvestre de Azevedo Martins, W. F. Carneiro, Bárbara do Carmo Rodrigues Virote, A. R. B. Vianna, L. D. Murgas
: The objective of this study was to develop a viable and low-cost model of voluntary physical exercise that could be applied to studies on metabolism and behavior. 40 male zebrafish (Danio rerio) were studied, divided into two groups: control and voluntary physical exercise. The model consisted of two aquariums connected by a translucent tube and a video camera on the side to measure physical exercise parameters of the animals. The animals showed higher acceleration and maximum speed and had a higher frequency of activity in the light period. In this model of voluntary physical exercise, we observed better performance in locomotor assessment tests, which was not accompanied by increased anxiety or changes in biochemical parameters related to lipid metabolism. Zebrafish responded positively to voluntary physical exercise and this model appears to be a good option for further studies.
{"title":"The Zebrafish (Danio rerio) as a Model for Studying Voluntary Physical Exercise and its Effects on Behavior and Metabolism","authors":"Moises Silvestre de Azevedo Martins, W. F. Carneiro, Bárbara do Carmo Rodrigues Virote, A. R. B. Vianna, L. D. Murgas","doi":"10.1590/1678-4324-2023220279","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220279","url":null,"abstract":": The objective of this study was to develop a viable and low-cost model of voluntary physical exercise that could be applied to studies on metabolism and behavior. 40 male zebrafish (Danio rerio) were studied, divided into two groups: control and voluntary physical exercise. The model consisted of two aquariums connected by a translucent tube and a video camera on the side to measure physical exercise parameters of the animals. The animals showed higher acceleration and maximum speed and had a higher frequency of activity in the light period. In this model of voluntary physical exercise, we observed better performance in locomotor assessment tests, which was not accompanied by increased anxiety or changes in biochemical parameters related to lipid metabolism. Zebrafish responded positively to voluntary physical exercise and this model appears to be a good option for further studies.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67462578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-13DOI: 10.1590/1678-4324-2023210786
Qingyao Li, Ting Lu, Xiaoyan Chen, Hongbiao Chu
: The essential oil from Citri Reticulatae Pericarpium (EOC) was analyzed by GC/MS. The major component of EOC was found to be d-limonene (92.3% of the total essential oil). Anti-ulcer and antioxidant activities of EOC were evaluated in several gastric ulcer models. The ulcer index or area and biochemical markers of superoxide dismutase (SOD) and malondialdehyde (MDA) were determined. EOC significantly inhibited gastric mucosal lesions induced by ulcerogenic agents with an inhibitory rate of nearly 50% at an oral dose of 85 mg/kg, and exerted a healing effect on acetic acid-induced ulcers with a curative rate of 63%. HCl/EtOH administration caused severe oxidative stress and lipid peroxidation, which was confirmed by significantly decreased SOD activity and increased MDA levels in mice gastric tissues. EOC pretreatment significantly increased SOD activity and decreased MDA levels, indicating that EOC protects the gastric mucosa against development of numerous lesions by inhibiting oxidative stress. These results demonstrate that EOC has anti-ulcerogenic activity and the mechanism underlying its gastric protective effect is associated with its antioxidant properties.
{"title":"Composition and Anti-ulcer Activity of the Essential Oil from Citri Reticulatae Pericarpium in Rodents","authors":"Qingyao Li, Ting Lu, Xiaoyan Chen, Hongbiao Chu","doi":"10.1590/1678-4324-2023210786","DOIUrl":"https://doi.org/10.1590/1678-4324-2023210786","url":null,"abstract":": The essential oil from Citri Reticulatae Pericarpium (EOC) was analyzed by GC/MS. The major component of EOC was found to be d-limonene (92.3% of the total essential oil). Anti-ulcer and antioxidant activities of EOC were evaluated in several gastric ulcer models. The ulcer index or area and biochemical markers of superoxide dismutase (SOD) and malondialdehyde (MDA) were determined. EOC significantly inhibited gastric mucosal lesions induced by ulcerogenic agents with an inhibitory rate of nearly 50% at an oral dose of 85 mg/kg, and exerted a healing effect on acetic acid-induced ulcers with a curative rate of 63%. HCl/EtOH administration caused severe oxidative stress and lipid peroxidation, which was confirmed by significantly decreased SOD activity and increased MDA levels in mice gastric tissues. EOC pretreatment significantly increased SOD activity and decreased MDA levels, indicating that EOC protects the gastric mucosa against development of numerous lesions by inhibiting oxidative stress. These results demonstrate that EOC has anti-ulcerogenic activity and the mechanism underlying its gastric protective effect is associated with its antioxidant properties.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67459670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.1590/1678-4324-2023220550
Neslihan Ay, Kaan Hürkan
: The pistachio nut ( Pistacia vera L.) is a popular nut which is beneficial for health. As a result of its high price, adulteration of pistachio products with other foods is common. The green pea ( Pisum sativum L.) is similar to pistachio in colour and texture when both are in a fine powder form, therefore powdered pistachio products are often adulterated with powdered green pea. In this study, we aimed to develop a novel method based on high resolution melting (HRM) to detect green pea adulteration in powdered pistachio products as low as in ratio. Novel primers that targeted the chloroplastidial sequence atp A, rpo C1 and ycf 3 were tested on admixture ratios of 90:10, 99:1, 499:1 and 999:1 of pistachio:green pea. The melting temperatures (Tm) were found to detect the adulteration for the targets rpo C1 and ycf 3. Since the atp A amplicons have identical guanine-cytosine content in both pistachio and green pea PCR products, it failed to distinguish the admixtures from the positive control samples. Differential plots and genotyping confidence percentages clearly distinguished the admixtures from positive controls down to a 0.1% ratio of pistachio:green pea. Sequencing of the amplicons supported the use of HRM to distinguish between pistachio and green pea. The use of HRM with novel rpoC1 and ycf3 primer sets was found to be useful for rapid, cost-effective, and reliable detection of green pea adulteration of pistachio down to a ratio of 0.1%.
{"title":"A Novel High-Resolution Melting Method for Detection of Adulteration on Pistachio (Pistacia vera L.)","authors":"Neslihan Ay, Kaan Hürkan","doi":"10.1590/1678-4324-2023220550","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220550","url":null,"abstract":": The pistachio nut ( Pistacia vera L.) is a popular nut which is beneficial for health. As a result of its high price, adulteration of pistachio products with other foods is common. The green pea ( Pisum sativum L.) is similar to pistachio in colour and texture when both are in a fine powder form, therefore powdered pistachio products are often adulterated with powdered green pea. In this study, we aimed to develop a novel method based on high resolution melting (HRM) to detect green pea adulteration in powdered pistachio products as low as in ratio. Novel primers that targeted the chloroplastidial sequence atp A, rpo C1 and ycf 3 were tested on admixture ratios of 90:10, 99:1, 499:1 and 999:1 of pistachio:green pea. The melting temperatures (Tm) were found to detect the adulteration for the targets rpo C1 and ycf 3. Since the atp A amplicons have identical guanine-cytosine content in both pistachio and green pea PCR products, it failed to distinguish the admixtures from the positive control samples. Differential plots and genotyping confidence percentages clearly distinguished the admixtures from positive controls down to a 0.1% ratio of pistachio:green pea. Sequencing of the amplicons supported the use of HRM to distinguish between pistachio and green pea. The use of HRM with novel rpoC1 and ycf3 primer sets was found to be useful for rapid, cost-effective, and reliable detection of green pea adulteration of pistachio down to a ratio of 0.1%.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67463580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.1590/1678-4324-2023210845
A. B. Pacheco, S. N. Duarte, Thaís da Silva Charles, J. G. Nascimento, A. Simões, J. E. Campos Júnior, A. Almeida, Hugo de Carvalho Ricardo, T. R. Lopes, F. L. F. Jesus, C. Lisboa
: The demands of high-quality zucchini fruits by the consumer market make studies regarding the improvement of cultivation systems, such as plant nutrition and salinity tolerance. Considering the demands of the consumer market for the high quality of zucchini fruits, it is necessary studies regarding the improvement of production systems for this improvement, such as nutrition and salinity tolerance to plant. Thus, this study aimed to evaluate the zucchini fruits quality characteristics, grown in soilless, under N:K ratios, and levels of electrical conductivity of the nutrient solution. The experiment was conducted in a protected environment in pots with 8.2 dm³ of volumetric capacity filled with commercial substrate. The trial design adopted was randomized blocks, with three replicates, in a 5x3 factorial scheme, with five levels of electrical conductivity (1.0; 2.0; 3.0; 4.0; and 5.0 dS m -1 ) and three N:K ratios (2:1; 1:1; and 1:2)
{"title":"Electrical Conductivity and Nitrogen:Potassium Ratios from Nutrigation in the Quality of Zucchini Fruits","authors":"A. B. Pacheco, S. N. Duarte, Thaís da Silva Charles, J. G. Nascimento, A. Simões, J. E. Campos Júnior, A. Almeida, Hugo de Carvalho Ricardo, T. R. Lopes, F. L. F. Jesus, C. Lisboa","doi":"10.1590/1678-4324-2023210845","DOIUrl":"https://doi.org/10.1590/1678-4324-2023210845","url":null,"abstract":": The demands of high-quality zucchini fruits by the consumer market make studies regarding the improvement of cultivation systems, such as plant nutrition and salinity tolerance. Considering the demands of the consumer market for the high quality of zucchini fruits, it is necessary studies regarding the improvement of production systems for this improvement, such as nutrition and salinity tolerance to plant. Thus, this study aimed to evaluate the zucchini fruits quality characteristics, grown in soilless, under N:K ratios, and levels of electrical conductivity of the nutrient solution. The experiment was conducted in a protected environment in pots with 8.2 dm³ of volumetric capacity filled with commercial substrate. The trial design adopted was randomized blocks, with three replicates, in a 5x3 factorial scheme, with five levels of electrical conductivity (1.0; 2.0; 3.0; 4.0; and 5.0 dS m -1 ) and three N:K ratios (2:1; 1:1; and 1:2)","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67460281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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