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Does antioxidant potential of traditional rice varieties vary with processing? 传统水稻品种的抗氧化能力是否随加工过程而变化?
Pub Date : 2020-08-24 DOI: 10.4038/ijms.v8i2.144
T. Thennakoon, S. Ekanayake
Antioxidants protect cells and tissues from free radical damage and therefore, are important in the prevention and management of a variety of chronic diseases. Bran of pigmented rice are potent sources of naturally occurring antioxidants. Rice being the staple diet of Sri Lankans and traditional rice gaining more attention at present, investigating the effect of processing and cooking on total phenol content and antioxidant potentials of selected traditional rice varieties were the aims of this study. Differently processed (raw undermilled, raw polished [4%] and parboiled undermilled) six rice varieties, namely, Godaheenati, Batapola el, Dik wee, Dahanala, Unakola samba and, Hangimuththan were used in the study. The antioxidant properties of rice flour extracted with phosphate buffer solution (PBS) were determined by 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, 1,1-diphenyl-2-picrylhydrazine (DPPH) radical scavenging activity and, ferric reducing antioxidant power (FRAP)assays. Moreover, the total polyphenolic content (TPC) of differently processed rice was analysed. Mean TPC of both uncooked and cooked, raw polished rice was the least (4.9-6.1 mg GAE/g) followed by parboiled (4.9-6.1 mg GAE/g). The highest TPC was in raw (5.3-6.7 mg GAE/g) rice. Mean ABTS activity of raw polished rice (0.8-1.9 mg TE/g) was the least followed by parboiled (1.2- 2.3 mg TE/g) and raw rice (1.3-2.1 mg TE/g). Mean DPPH scavenging and FRAP activities followed the same pattern with raw rice having the highest (4.5-6.2 mg AE/100g; 4.6-14.4 mg AE/100g) followed by parboiled (4.4-5.1 mg AE/100g); 5.0-15.2 mg AE/100g) and the least in raw polished (4.0-4.6 mg AE/100g; 5.1-18.5 mg AE/100g) respectively. Phenolic compounds and antioxidant potential increased in the order of raw polished, parboiled, and raw rice flour in both cooked and uncooked rice. Rice grains with red coloured bran produced higher antioxidant activity compared to varieties with white bran. However, cooking reduced the antioxidant potentials in all differently processed varieties.
抗氧化剂保护细胞和组织免受自由基损伤,因此,在预防和管理各种慢性疾病中很重要。有色米的麸皮是天然抗氧化剂的有效来源。水稻是斯里兰卡人的主食,传统水稻也越来越受到重视,研究加工和蒸煮对传统水稻品种总酚含量和抗氧化能力的影响是本研究的目的。研究中使用了六种不同加工方式(未经碾磨、未经抛光[4%]和半煮未碾磨)的大米品种,即Godaheenati、Batapola el、Dik wee、Dahanala、Unakola samba和Hangimuththan。采用磷酸缓冲液(PBS)提取的米粉,通过2,2′-氮基-双(3-乙基苯并噻唑-6-磺酸)(ABTS)自由基清除能力、1,1-二苯基-2-吡啶肼(DPPH)自由基清除能力和铁还原抗氧化能力(FRAP)测定其抗氧化性能。并对不同加工方式大米的总多酚含量进行了分析。生精米和熟精米的平均TPC最低(4.9-6.1 mg GAE/g),其次是煮熟的米(4.9-6.1 mg GAE/g)。TPC以生米最高(5.3 ~ 6.7 mg GAE/g)。粗精米(0.8 ~ 1.9 mg TE/g)的平均ABTS活性最低,其次是半熟米(1.2 ~ 2.3 mg TE/g)和粗米(1.3 ~ 2.1 mg TE/g)。平均DPPH清除率和FRAP活性均以糙米最高(4.5 ~ 6.2 mg AE/100g;4.6 ~ 14.4 mg AE/100g),其次是半煮(4.4 ~ 5.1 mg AE/100g);5.0-15.2 mg AE/100g),生料中AE最少(4.0-4.6 mg AE/100g;5.1 ~ 18.5 mg AE/100g)。熟米和生米中酚类化合物和抗氧化潜力依次增加,依次为生磨米、半煮米和生米粉。红色米糠比白色米糠具有更高的抗氧化活性。然而,烹饪降低了所有不同加工品种的抗氧化能力。
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引用次数: 3
Comparative study on operation theatre facility utilization in government hospitals and semi-government hospitals 公立医院与半公立医院手术室设施利用的比较研究
Pub Date : 2020-08-24 DOI: 10.4038/ijms.v8i2.138
E. K. Wellala, G. Dharmaratne
The operation theatre (OT) complex is a costly component of a hospital budget expenditure. This area of hospital activity requires maximum utilization to ensure optimum cost-benefit ratio. Two tertiary care centers in the Colombo district, Sri Jayewardenepura General Hospital (SJGH), the only semi-government tertiary care center in Sri Lanka, and Colombo South Teaching Hospital (CSTH) providing similar service delivery to government hospitals were studied to compare utilization patterns. The results showed no significant statistical difference in average per-bed utilization of SJGH and CSTH (P=0.499). The average per-bed utilization rate at CSTH was 23.26%, and SJGH was 25.66%. There was a significant statistical difference in the average per-bed capacity utilization of operating theaters at CSTH and SJGH. The average per bed capacity utilization time per day was significantly higher at SJGH (P=0.040). The average per-bed capacity utilization rate at CSTH was 11.63% and 15.2% at SJGH. According to the Consultants' perception of the importance of factors affecting utilization, less significance is given to time management and related administrative functions even though they have the highest correlation to utilization factors. Utilization observed at both hospitals was lower than the global benchmark between 70% and 80%. The study identified the importance of factors affecting utilization towards human resource availability, motivation, training needs and equipment, and resource availability. Surgeons have given less priority to time management and related administrative functions though the literature suggests time management and relative administratively modifiable factors as the most critical factors. (Gupta, 2011). Therefore, it is recommended to address the factors such as “cancellation of planned surgeries”, “accurate prediction of procedure times in scheduling theatre lists”, “convenient theater time schedules with other clinical work”, “patient turn over time”, and “patient transport time from wards” as they can result in overall improvement in operating facility utilization.
手术室(OT)综合体是医院预算支出中昂贵的组成部分。医院活动的这一领域需要最大限度地利用,以确保最佳的成本效益比。对科伦坡地区的两个三级保健中心Sri Jayewardenepura总医院(SJGH)(斯里兰卡唯一的半政府三级保健中心)和科伦坡南教学医院(CSTH)(提供与政府医院类似的服务)进行了研究,以比较利用模式。结果显示,SJGH和CSTH的平均床位利用率差异无统计学意义(P=0.499)。CSTH的平均床位利用率为23.26%,SJGH的平均床位利用率为25.66%。中院与上海综合医院手术室平均床位利用率差异有统计学意义。SJGH的平均每日病床容量利用时间显著高于SJGH (P=0.040)。中港医院的平均床位使用率为11.63%,上海医院则为15.2%。根据顾问对影响利用的因素的重要性的看法,时间管理和有关的行政职能的重要性较低,尽管它们与利用因素的相关性最高。在这两家医院观察到的利用率低于70%至80%的全球基准。这项研究确定了影响利用的因素对人力资源的可得性、动机、培训需要和设备以及资源的可得性的重要性。尽管文献表明时间管理和相关的行政管理因素是最关键的因素,但外科医生对时间管理和相关行政管理功能的重视程度较低。(古普塔,2011)。因此,建议解决“计划手术取消”、“在安排手术室名单时准确预测手术时间”、“方便与其他临床工作安排的手术室时间”、“患者转诊时间”、“患者从病房转移时间”等因素,从而提高手术设施的整体利用率。
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引用次数: 0
Taste of architectural space in real estate development: Review the concept of an emerging phenomenon 房地产开发中的建筑空间品味:回顾一种新兴现象的概念
Pub Date : 2020-08-24 DOI: 10.4038/ijms.v8i2.143
H. Herath, M. A. N. Rasanjalee Perera, D. P. Chadrasekara
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引用次数: 0
English language teaching in government schools in Sri Lanka: a survey based on Kinniya Education Zone, Trincomalee 斯里兰卡公立学校的英语教学:基于亭可马里金尼亚教育区的调查
Pub Date : 2020-08-24 DOI: 10.4038/ijms.v8i2.140
A. F. Abdul Halik
A This study was carried out with the aim of investigating the state of ELT in Kinniya Education Zone, Trincomalee and the factors affecting ELT. This study was a survey research and quantitative method was used for primary data collection. Kinniya Education Zone was selected as the research setting and 35 English as a Second Language teachers from 35 town schools and rural schools were randomly selected as sample population. A questionnaire with close-ended questions was used as the research instrument. Based on the findings of the data analysis, most of the participants’ educational qualifications were Higher National Diploma in English (HNDE) and English medium degrees. Moreover, a large number of students agreed that the ESL teachers in Kinniya Education Zone encounter challenges in teaching English. Lack of interest, lack of basic knowledge in English, crowded classrooms, use of traditional teaching methods and mother tongue and lack of modern teaching/learning equipment are identified as major factors for the failure of ELT in Kinniya Education Zone. According to participants’ responses, the following suggestions can be implemented at government schools in Sri Lanka to overcome the failure of ELT in Kinniya Education Zone. They are: use of modern teaching approaches, establishing a separate language unit with modern teaching equipment in each school, appointing competent and trained teachers at primary sections/schools, using English Language in the classrooms, reducing the number of students in the crowded classes and application of interesting language games and activities in the classroom.
本研究旨在调查亭可马里肯尼亚教育区英语教学现状及影响英语教学的因素。本研究为调查研究,主要资料收集采用定量方法。选取肯尼亚教育区作为研究场所,随机抽取35所城镇学校和农村学校的35名英语作为第二语言教师作为样本人群。采用封闭式问题问卷作为研究工具。根据数据分析的结果,大多数参与者的教育资格是国家英语高级文凭(HNDE)和英语中等学位。此外,很多学生都认为肯尼亚教育区的ESL教师在英语教学中遇到了挑战。缺乏兴趣,缺乏英语基础知识,教室拥挤,使用传统的教学方法和母语,缺乏现代化的教学设备是Kinniya教育区英语教学失败的主要因素。根据与会者的反馈,以下建议可以在斯里兰卡的政府学校实施,以克服Kinniya教育区的英语教学失败。它们是:使用现代化的教学方法,在每个学校建立一个独立的语言单位并配备现代化的教学设备,在小学部/学校任命称职和训练有素的教师,在课堂上使用英语,减少拥挤班级的学生人数,在课堂上应用有趣的语言游戏和活动。
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引用次数: 1
The prevalence and correlates of depressive symptoms in patients with cancer treated in a Tertiary health care facility in Southern Sri Lanka 斯里兰卡南部三级卫生保健机构治疗的癌症患者抑郁症状的患病率及其相关因素
Pub Date : 2020-08-24 DOI: 10.4038/ijms.v8i2.137
E. Weeratunga, C. Senadheera, H. Hettiarachchi, B. Perera
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引用次数: 2
Dialectic nature of digital culture: theoretical analysis of evolutionary ‘digital Buddhism’ of social media debate 数字文化的辩证法本质:对社交媒体辩论中演化的“数字佛教”的理论分析
Pub Date : 2020-08-24 DOI: 10.4038/ijms.v8i2.141
W. Wijayarathna, P. Abhayasundere, P. Jayaweera
Popular Buddhism is one of the key cultural aspects in present Sri Lankan society.  Its main cause is the characteristics of the path of the Buddhism which it currently travels. Digital anthropology is a field that studies the complex relationships between human culture and the concept of digital. Prominently, digital is a concept based on binary numbers which means 1 and 0. This research aims to provide some insight into the behavior of digital within the social institution of religion. To accomplish these objectives the researchers utilized a Facebook page (Siri Sadaham Ashramaya) and conducted an analysis through the comments from its purposively selected post and it was based on speech act theory elaborated by J. L. Austin (1962) and John Searle (1975). The theoretical approach for this research is the first principle of digital anthropology: dialectic nature of digital culture, developed by Daniel Miller and Heather Horst. A hypothesis was applied as: modern popular Buddhism expand the dialectic nature of culture through social media that consisted of thesis, antithesis, and synthesis as Hegal and Marx stated. The results of the study proved the hypothesis and the opinions of the respective audience’s reactions and comments among the applied Facebook page, and emphasized the dialectical nature of digital culture which means the contribution to the increment of logical background of the culture. Consequently, this study suggests that the modern popular Buddhism concept evolved into a novel concept as “Digital Buddhism” and it can be defined as a new division of Sri Lankan Buddhism as proposed by Gananth Obeyesekere and Richard Gombrich during 1988s. Researchers propose a newly designed model to analyze the digital nature and the theoretical perspective of digital anthropology utilizing the nature of modern popular Buddhism. KEYWORDS:  anthropology, digital, dialectic, popular Buddhism, culture
民间佛教是当今斯里兰卡社会重要的文化方面之一。其主要原因是其目前所走的佛教道路的特点。数字人类学是研究人类文化与数字概念之间复杂关系的学科。突出的是,数字是一个基于二进制数的概念,即1和0。本研究旨在为宗教社会制度下的数字行为提供一些见解。为了实现这些目标,研究人员利用了一个Facebook页面(Siri Sadaham Ashramaya),并通过有目的选择的帖子的评论进行了分析,这是基于J. L. Austin(1962)和John Searle(1975)阐述的言语行为理论。这项研究的理论方法是数字人类学的第一原则:数字文化的辩证法本质,由丹尼尔·米勒和希瑟·霍斯特提出。一个假设是:现代大众佛教通过黑格尔和马克思所说的由正、反、合组成的社交媒体,扩展了文化的辩证法本质。研究结果证明了假设和各自受众在应用Facebook页面中的反应和评论意见,并强调了数字文化的辩证性质,即对文化逻辑背景增量的贡献。因此,本研究表明,现代流行佛教概念演变为一个新颖的概念,即 - œDigital佛教 -,它可以被定义为Gananth Obeyesekere和Richard Gombrich在20世纪80年代提出的斯里兰卡佛教的新分支。研究人员利用现代大众佛教的本质,提出了一种新的模型来分析数字本质和数字人类学的理论视角。关键词:人类学,数字化,辩证法,大众佛教,文化
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引用次数: 1
Effect of selected water quality parameters on the prevalence of Poecilia reticulata (Guppy) population in Sri Jayewardenepura canal system 选定水质参数对Sri Jayewardenepura运河系统孔雀鱼种群流行的影响
Pub Date : 2020-08-24 DOI: 10.4038/ijms.v8i2.139
P. Pethiyagoda, S. De Alwis, B. D. de Silva
Present study was carried out to determine the effect of some selected water quality parameters on the prevalence of P. reticulata(Guppy) population in Sri Jayewardenepura canal system. Fish and water samples were collected in six locations from January 2016 to December 2017. Dissolved Oxygen (DO), Biochemical Oxygen Demand (BOD), Electrical Conductivity (EC), Hardness, Alkalinity, Nitrate-Nitrogen (N-NO 3 - ), Orthophosphate (PO 4 3- ) ,Temperature, pH ,Water velocity and depths in each sites were measured. Total lengths and weights of specimens (n-520) were measured and relative densities (RD) were calculated. Guppy was the most abundant species in all sites and high densities were recorded at slow moving and stagnated shallow waters. Results revealed that all the sites were subjected to moderate or high pollution and most conspicuous were the low DO (0.83±0.12-4.84±0.76 mg/l) and high BOD (1.78±0.4-9.10±0.6 mg/l) levels. Polluted water quality of studied locations were further confirmed by high values for (N-NO3-) (3.9±0.8-11.5±1.2 mg/l), PO43- (1.9±0.4-3.9±0.3mg/l) and EC (257.6±12.8-801.6±42.0µS/cm). Other recorded water quality parameters pH (6.6±0.2– 8.3±0.4), Temperature (29.9±1.1-8.3±0.4), Hardness (80.0±10.4- 113.3±13.1 mg/l) and Alkalinity (70.7±11.5-179.6±20.4 mg/l) are in suitable range of fish and aquatic life. Despite the fact that the locations were polluted, RD of guppy (32.7±27.7-85.7±22.9) were considerable in all locations. The study showed that the RD of guppy significantly varied with some of the water quality parameters; decreasing with increasing DO, N-NO 3- ,PO 43- levels (P≤0.05) as well as with water depths and velocity of water and increasing with increasing pH, BOD and alkalinity of water. The study recorded that the average adult sizes of fish were smaller (♀-35 mm, ♂-25 mm) than the previous records (♀-60 mm, ♂-40 mm) in the present study area. Thus, study concludes that, although the water quality parameters are affecting their growth rates and relative densities.
本研究旨在确定若干水质参数对Sri Jayewardenepura运河系统中孔雀鱼(P. reticulata, Guppy)种群流行的影响。2016年1月至2017年12月在六个地点采集了鱼和水样本。测定各测点的溶解氧(DO)、生化需氧量(BOD)、电导率(EC)、硬度、碱度、硝酸盐氮(n - no3 -)、正磷酸盐(po4 - 3-)、温度、pH、流速和深度。测量了n-520标本的总长度和总重量,并计算了相对密度。孔雀鱼是所有地点最丰富的物种,在缓慢移动和停滞的浅水中记录到高密度。结果表明,各试验点均为中度或重度污染,其中以低DO(0.83±0.12 ~ 4.84±0.76 mg/l)和高BOD(1.78±0.4 ~ 9.10±0.6 mg/l)污染最为显著。研究地点的污染水质进一步证实了(N-NO3-)(3.9±0.8 ~ 11.5±1.2 mg/l)、PO43-(1.9±0.4 ~ 3.9±0.3mg/l)和EC(257.6±12.8 ~ 801.6±42.0µS/cm)的高值。其他记录的水质参数pH值(6.6±0.2 -8.3±0.4)、温度(29.9±1.1-8.3±0.4)、硬度(80.0±10.4- 113.3±13.1 mg/l)和碱度(70.7±11.5-179.6±20.4 mg/l)均在鱼类和水生生物的适宜范围内。尽管这些地点受到污染,但孔雀鱼的RD(32.7±27.7 ~ 85.7±22.9)在所有地点都相当可观。研究表明,孔雀鱼的RD随某些水质参数的变化有显著差异;随DO、n - no3 -、po43水平的增加而降低(P≤0.05),随水体深度和流速的增加而增加,随水体pH、BOD和碱度的增加而增加。研究发现,在本研究区域,鱼的平均成年尺寸(♀-35 mm,♂-25 mm)比以前的记录(♀-60 mm,♂-40 mm)要小。因此,研究得出结论,虽然水质参数影响它们的生长速度和相对密度。
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引用次数: 0
Projected extreme rainfall indices in Guinea and Sudano-sahelian Ecological Zones, Nigeria 尼日利亚几内亚和苏丹-萨赫勒生态区极端降雨指数预估
Pub Date : 2020-06-29 DOI: 10.4038/ijms.v7i1.120
A. Salihu, A. Abdulkadir, G. N. Nsofor, M. Y. Otache
Drought and Flood episodes are twin issues that are consequences of extreme rainfall events. The negative impact of extreme rainfall events makes understanding their behaviour under the future climate change necessary for regional planning. Hence, the objective of the study is to project extreme rainfall indices in Guinea and Sudano-Sahelian ecological zones, Nigeria. A set of four extreme rainfall indices namely: maximum 5-day rainfall (Rx5day), heavy rainfall days (R10mm), consecutive wet days (CWD) and consecutive dry days (CDD) were adopted. The data and computation were done using KNMI Climate Explorer. Projections were produced for the near-term 2019-2048, mid-term 2049-2078 and long-term 2079-2100 periods with reference to the 1959-1988 and 1989-2018 baselines. The multi-model ensemble mean of couple model intercomparison project 5 (CMIP5) under representative concentration pathways (RCPs) 2.6, 4.5, and 8.5 were used. Mann-Kendal statistical test was adopted to analyze the trends in extreme rainfall indices at the 0.05 significance level. Based on the results, it can be deduced that there is a significant positive trend in the whole Guinea and Sudano-Sahelian ecological zone as a region for maximum 5-day rainfall with respect to all the three RCPs. As for heavy rainfall, itreveals that there is no significant positive trend for RCP2.6 with respect to the three projected periods under consideration but significant positive trends with respect to 2049-2078 for RCP4.5 as well as RCP8.5 with respect to 2049-2078 and 2079-2100 periods. Increase in CDD, as well as a decrease in CWD, were both not significant at the 0.05 confidence level. Therefore, it is expected that this study will aid guidance to the understanding of the ongoing changes as well as possible changes in rainfall and rainfall-related extremes. It is also important for future planning of water resources management and agriculture in Guinea and Sudano-Sahelian ecological zones of Nigeria.
干旱和洪水是极端降雨事件的双重后果。极端降雨事件的负面影响使得了解其在未来气候变化下的行为成为区域规划的必要条件。因此,本研究的目的是预测几内亚和尼日利亚苏丹-萨赫勒生态区的极端降雨指数。采用5天最大降雨量(Rx5day)、强降雨日数(R10mm)、连续湿日数(CWD)和连续干日数(CDD) 4个极端降雨指标。数据和计算使用KNMI气候探索者完成。根据1959-1988年和1989-2018年的基线,对2019-2048年的短期、2049-2078年的中期和2079-2100年的长期进行了预测。采用典型浓度路径(rcp) 2.6、4.5和8.5下的耦合模式比对项目5 (CMIP5)的多模式集合平均值。采用Mann-Kendal统计检验,在0.05显著水平上分析极端降雨指数变化趋势。结果表明,整个几内亚和苏丹-萨赫勒生态区作为最大5天降雨量区域,在所有3个区域均呈现显著的正趋势。对于强降水,RCP2.6相对于所考虑的三个预估期没有显著的正趋势,而RCP4.5和RCP8.5相对于2049 ~ 2078和2079 ~ 2100期有显著的正趋势。CDD的增加和CWD的减少在0.05的置信水平上均不显著。因此,预计这项研究将有助于指导人们了解降雨和与降雨有关的极端事件的持续变化以及可能的变化。这对几内亚和尼日利亚的苏丹-萨赫勒生态区的水资源管理和农业的未来规划也很重要。
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引用次数: 0
Antimicrobial properties of ethanolic and methanolic extracts of finger millet (Eleusine coracana (L.) Gaertn.) varieties cultivated in Sri Lanka 小谷乙醇和甲醇提取物的抗菌性能研究在斯里兰卡栽培的品种
Pub Date : 2020-06-29 DOI: 10.4038/ijms.v7i1.123
S. Jayawardana, J. Samarasekera, G. Hettiarachchi, J. Gooneratne, R. Maharjan, M. Choudhary
The emerging significance of natural antimicrobial agents creates an imperative need to identify novel plant sources with antimicrobial activities. The objective of the present study was to evaluate antibacterial, antifungal and β-lactamase enzyme inhibitory activities of ethanolic and methanolic extracts of Ravi, Rawana and Oshadha finger millet varieties. Flours of whole grains of the finger millet varieties were extracted with absolute ethanol and methanol separately. Antibacterial activities against six antibiotic-sensitive and four antibiotic-resistant pathogenic bacterial strains were evaluated using the resazurin reduction assay. Antifungal activities against six antimicrobial-sensitive pathogenic fungal strains were evaluated using the agar tube dilution method. β-Lactamase enzyme inhibitory activity was evaluated using a standard method. Both ethanolic and methanolic extracts of the three finger millet varieties showed dose-dependent inhibitory activities against the tested antibiotic-sensitive and antibiotic-resistant bacterial strains while exhibiting high inhibitions against Gram-positive antibiotic-sensitive bacterial strains when compared to Gram-negative antibiotic-sensitive bacterial strains. The findings revealed the antibacterial potential of both ethanolic and methanolic extracts of the three finger millet varieties against antibiotic-sensitive Staphylococcus aureus (ATCC ® 6538™) and Bacillus subtilis (ATCC ® 23857™) strains and the minimum inhibitory concentrations of the extracts against S. aureus and B. subtilis were found to be 2.1 and 1.8 mg/ml, respectively. However, none of the extracts can be considered as significantly active against the tested antibiotic-sensitive and antibiotic-resistant bacterial strains when compared to the standard drugs. In addition, none of the extracts can be considered as active against the tested fungal strains at the tested concentrations. Nevertheless, all extracts showed more activities against the tested bacterial strains when compared to the tested fungal strains. Since all extracts showed less than 40% β-lactamase inhibitory activities even at 2 mg/ml concentration, they do not qualify as promising sources of β-lactamase inhibitors at the tested concentration.
天然抗菌剂的新意义创造了迫切需要识别具有抗菌活性的新型植物来源。研究了拉维、拉瓦纳和奥沙达三种手指粟乙醇和甲醇提取物的抑菌、抗真菌和β-内酰胺酶抑制活性。用无水乙醇和甲醇分别提取五指粟全粒面粉。采用瑞祖脲还原法对6株抗生素敏感菌和4株抗生素耐药菌进行抑菌活性评价。采用琼脂管稀释法对6株抗菌敏感病原菌进行抑菌活性评价。采用标准方法测定β-内酰胺酶抑制活性。三种小谷子品种的乙醇和甲醇提取物对抗生素敏感和耐药菌株均表现出剂量依赖性的抑制活性,而对革兰氏阳性抗生素敏感菌株的抑制活性高于革兰氏阴性抗生素敏感菌株。结果表明,三种五指粟品种乙醇和甲醇提取物对抗生素敏感的金黄色葡萄球菌(ATCC®6538™)和枯草芽孢杆菌(ATCC®23857™)均有抑菌作用,对金黄色葡萄球菌和枯草芽孢杆菌的最低抑菌浓度分别为2.1和1.8 mg/ml。然而,与标准药物相比,没有一种提取物可以被认为对所测试的抗生素敏感和抗生素耐药菌株具有显着活性。此外,在测试浓度下,没有一种提取物可以被认为对测试的真菌菌株有活性。然而,所有提取物对所测试的细菌菌株的活性都高于所测试的真菌菌株。由于所有提取物即使在2 mg/ml浓度下也显示出低于40%的β-内酰胺酶抑制活性,因此在测试浓度下,它们不符合β-内酰胺酶抑制剂的有希望的来源。
{"title":"Antimicrobial properties of ethanolic and methanolic extracts of finger millet (Eleusine coracana (L.) Gaertn.) varieties cultivated in Sri Lanka","authors":"S. Jayawardana, J. Samarasekera, G. Hettiarachchi, J. Gooneratne, R. Maharjan, M. Choudhary","doi":"10.4038/ijms.v7i1.123","DOIUrl":"https://doi.org/10.4038/ijms.v7i1.123","url":null,"abstract":"The emerging significance of natural antimicrobial agents creates an imperative need to identify novel plant sources with antimicrobial activities. The objective of the present study was to evaluate antibacterial, antifungal and β-lactamase enzyme inhibitory activities of ethanolic and methanolic extracts of Ravi, Rawana and Oshadha finger millet varieties. Flours of whole grains of the finger millet varieties were extracted with absolute ethanol and methanol separately. Antibacterial activities against six antibiotic-sensitive and four antibiotic-resistant pathogenic bacterial strains were evaluated using the resazurin reduction assay. Antifungal activities against six antimicrobial-sensitive pathogenic fungal strains were evaluated using the agar tube dilution method. β-Lactamase enzyme inhibitory activity was evaluated using a standard method. Both ethanolic and methanolic extracts of the three finger millet varieties showed dose-dependent inhibitory activities against the tested antibiotic-sensitive and antibiotic-resistant bacterial strains while exhibiting high inhibitions against Gram-positive antibiotic-sensitive bacterial strains when compared to Gram-negative antibiotic-sensitive bacterial strains. The findings revealed the antibacterial potential of both ethanolic and methanolic extracts of the three finger millet varieties against antibiotic-sensitive Staphylococcus aureus (ATCC ® 6538™) and Bacillus subtilis (ATCC ® 23857™) strains and the minimum inhibitory concentrations of the extracts against S. aureus and B. subtilis were found to be 2.1 and 1.8 mg/ml, respectively. However, none of the extracts can be considered as significantly active against the tested antibiotic-sensitive and antibiotic-resistant bacterial strains when compared to the standard drugs. In addition, none of the extracts can be considered as active against the tested fungal strains at the tested concentrations. Nevertheless, all extracts showed more activities against the tested bacterial strains when compared to the tested fungal strains. Since all extracts showed less than 40% β-lactamase inhibitory activities even at 2 mg/ml concentration, they do not qualify as promising sources of β-lactamase inhibitors at the tested concentration.","PeriodicalId":91884,"journal":{"name":"International journal of current multidisciplinary studies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87463652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Assessment of heavy metal contamination in the sediments of Meda - Ela canal near Karadiyana semi-controlled dumpsite, Sri Lanka 斯里兰卡卡拉迪亚纳半控制垃圾场附近梅达-埃拉运河沉积物中重金属污染的评估
Pub Date : 2020-06-29 DOI: 10.4038/ijms.v7i1.118
S. Koliyabandara, A. Cooray, S. Liyanage, C. Siriwardana
The pollution of natural waterbodies with landfill leachate from dumpsites is a severe environmental issue all over the world. Karadiyana semi-controlled dumpsite in the Western province of Sri Lanka is one of the largest dumpsites in the country. The leachate generated in the dumpsite directly flows into Meda Ela canal which is a tributary of Bolgoda Lake Weras Ganga system causing a significant pollution threat on aquatic life. The current study assesses the contamination level of selected heavy metals in the sediments of Meda-Ela close to the dumpsite. The study was carried out for a period of one year from January - December 2018. The sediment samples were characterized with an alkaline pH ranging from 8.40 to 8.70. The electrical conductivity of the sediment was recorded with a mean value of 139.93 ± 26.7 µS/cm. The mean concentration of Cu in sediment was of 73.53± 2.52 mg/Kg and it falls into the EPA guideline for heavily polluted sediments. Total Cu concentration exceeds the threshold effect concentration but falls below the probable effect concentration. The total Fe concentration was relatively high (110.83 ±1.96 mg/Kg) among the measured metal species. The other metals, Cd, Cr and Zn was recorded with mean concentrations of 0.38 ± 0.03,103 ± 41.62, 94.4 ± 2.08 mg/Kg respectively. Pollution Load Index of the sediment was 0.87 and therefore it comes under no pollution category. This suggests that there is no appreciable input from anthropogenic sources. Based on geo- accumulation index, sediments are moderately polluted with respect to Cu and Cd.
垃圾填埋场渗滤液对天然水体的污染是一个全球性的严重环境问题。位于斯里兰卡西部省份的Karadiyana半控制垃圾场是该国最大的垃圾场之一。垃圾场产生的渗滤液直接流入梅达埃拉运河,这条运河是博尔戈达湖和恒河系统的一条支流,对水生生物造成了严重的污染威胁。目前的研究评估了靠近垃圾场的Meda-Ela沉积物中选定重金属的污染水平。该研究于2018年1月至12月进行了为期一年的研究。沉积物样品的碱性pH值在8.40 ~ 8.70之间。测得沉积物电导率平均值为139.93±26.7µS/cm。沉积物中Cu的平均浓度为73.53±2.52 mg/Kg,符合EPA对重度污染沉积物的指导标准。总Cu浓度超过阈值效应浓度,但低于可能效应浓度。总铁浓度较高(110.83±1.96 mg/Kg)。其他金属Cd、Cr、Zn的平均浓度分别为0.38±0.03、103±41.62、94.4±2.08 mg/Kg。沉积物的污染负荷指数为0.87,属于无污染范畴。这表明没有明显的人为来源的输入。根据地质堆积指数,沉积物中Cu和Cd的污染程度为中度。
{"title":"Assessment of heavy metal contamination in the sediments of Meda - Ela canal near Karadiyana semi-controlled dumpsite, Sri Lanka","authors":"S. Koliyabandara, A. Cooray, S. Liyanage, C. Siriwardana","doi":"10.4038/ijms.v7i1.118","DOIUrl":"https://doi.org/10.4038/ijms.v7i1.118","url":null,"abstract":"The pollution of natural waterbodies with landfill leachate from dumpsites is a severe environmental issue all over the world. Karadiyana semi-controlled dumpsite in the Western province of Sri Lanka is one of the largest dumpsites in the country. The leachate generated in the dumpsite directly flows into Meda Ela canal which is a tributary of Bolgoda Lake Weras Ganga system causing a significant pollution threat on aquatic life. The current study assesses the contamination level of selected heavy metals in the sediments of Meda-Ela close to the dumpsite. The study was carried out for a period of one year from January - December 2018. The sediment samples were characterized with an alkaline pH ranging from 8.40 to 8.70. The electrical conductivity of the sediment was recorded with a mean value of 139.93 ± 26.7 µS/cm. The mean concentration of Cu in sediment was of 73.53± 2.52 mg/Kg and it falls into the EPA guideline for heavily polluted sediments. Total Cu concentration exceeds the threshold effect concentration but falls below the probable effect concentration. The total Fe concentration was relatively high (110.83 ±1.96 mg/Kg) among the measured metal species. The other metals, Cd, Cr and Zn was recorded with mean concentrations of 0.38 ± 0.03,103 ± 41.62, 94.4 ± 2.08 mg/Kg respectively. Pollution Load Index of the sediment was 0.87 and therefore it comes under no pollution category. This suggests that there is no appreciable input from anthropogenic sources. Based on geo- accumulation index, sediments are moderately polluted with respect to Cu and Cd.","PeriodicalId":91884,"journal":{"name":"International journal of current multidisciplinary studies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73051427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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International journal of current multidisciplinary studies
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