Pub Date : 2022-07-31DOI: 10.24070/bjvp.1983-0246.v15i2p83-92
L. Burns, A. Veiga, S. Moron, F. Cordova, Domenica de Souza, E. Viana, F. Zimermann, Rosane Gomes da Silva, L. Sousa, C. D. de Cordova, Sílvia Barbosa, A. Ramos
This study aimed to evaluate the effects of stress in bovines submitted to emergency slaughter caused by lengthy transportation to slaughterhouses in the state of Tocantins, Brazil. Blood collections were performed individually during exsanguinations. Tissue samples were obtained after evisceration on the slaughter line. The animals were divided into the Experimental Group (EG), with 19 bovines destined for immediate emergency slaughter, and the Control Group (CG), with 24 bovines slaughtered normally at the abattoir flow. The EG showed lower values of erythrocytes, hemoglobin, hematocrit, platelets, neutrophilia with band neutrophils, and lymphopenia. Hyperalbuminemia, high levels of total proteins, glucose, and creatinine, and lower urea levels were observed in the EG. The globulins were equal between the groups. Histopathology revealed hepatocytes with vacuolated cytoplasm (87.5% of the cases) and liver congestion (83.33%). Interstitial emphysema (95.65%), alveolar emphysema (65.21%), and congestion (52.17%) were observed in the lungs. The kidneys showed congestion (80.95%) and hyaline casts (100%). The spleens showed rarefaction in the white (70%) and red pulp (65%), in addition to hemosiderosis (10%). This suggests that the animals transported over long distances are submitted to severe stress, with water and food deprivation, resulting in hematological and biochemical changes, with histological lesions and serious bruises affecting the musculoskeletal system. There is loss of homeostasis, severe debilitation, with destination for emergency slaughter with the conditional use of meat for consumption.
{"title":"Hematological, biochemical and histopathological changes in cattle submitted to emergency slaughter","authors":"L. Burns, A. Veiga, S. Moron, F. Cordova, Domenica de Souza, E. Viana, F. Zimermann, Rosane Gomes da Silva, L. Sousa, C. D. de Cordova, Sílvia Barbosa, A. Ramos","doi":"10.24070/bjvp.1983-0246.v15i2p83-92","DOIUrl":"https://doi.org/10.24070/bjvp.1983-0246.v15i2p83-92","url":null,"abstract":"This study aimed to evaluate the effects of stress in bovines submitted to emergency slaughter caused by lengthy transportation to slaughterhouses in the state of Tocantins, Brazil. Blood collections were performed individually during exsanguinations. Tissue samples were obtained after evisceration on the slaughter line. The animals were divided into the Experimental Group (EG), with 19 bovines destined for immediate emergency slaughter, and the Control Group (CG), with 24 bovines slaughtered normally at the abattoir flow. The EG showed lower values of erythrocytes, hemoglobin, hematocrit, platelets, neutrophilia with band neutrophils, and lymphopenia. Hyperalbuminemia, high levels of total proteins, glucose, and creatinine, and lower urea levels were observed in the EG. The globulins were equal between the groups. Histopathology revealed hepatocytes with vacuolated cytoplasm (87.5% of the cases) and liver congestion (83.33%). Interstitial emphysema (95.65%), alveolar emphysema (65.21%), and congestion (52.17%) were observed in the lungs. The kidneys showed congestion (80.95%) and hyaline casts (100%). The spleens showed rarefaction in the white (70%) and red pulp (65%), in addition to hemosiderosis (10%). This suggests that the animals transported over long distances are submitted to severe stress, with water and food deprivation, resulting in hematological and biochemical changes, with histological lesions and serious bruises affecting the musculoskeletal system. There is loss of homeostasis, severe debilitation, with destination for emergency slaughter with the conditional use of meat for consumption.","PeriodicalId":9223,"journal":{"name":"Brazilian Journal of Veterinary Pathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42324218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-31DOI: 10.24070/bjvp.1983-0246.v15i2p121-123
F. Navolar, Julio Kioquetta, M. Ribeiro, A. Dearo, T. Faccin
Clinical history: A 4-year-old Quarter Horse stallion was unsuccessfully treated for a circumscribed and ulcerated lesion of unknown duration located lateral to the prepuce. Microscopic description: The superficial and deep preputial dermis contains multifocal to coalescing, well-demarcated eosinophilic granulomas characterized by a central area of abundant eosinophilic necrotic debris mixed with degenerate eosinophils surrounded by numerous epithelioid macrophages and occasional multinucleated giant cells (foreign-body type), rimmed by ill-defined layers of fibrous connective tissue. In the center of the granulomas, there are cross-sections of degenerate nematode larvae. The adjacent dermal collagen is expanded by dense inflammatory infiltrate composed mainly of eosinophils and a few lymphocytes and plasma cells. The dermal microvasculature is prominent, with hypertrophic reactive endothelial lining. There is also vascular proliferation amidst perpendicularly oriented plump fibroblasts embedded in collagen (granulation tissue). In addition, there are numerous shrunken, hypereosinophilic, fragmented or hyalinized collagen bundles, occasionally surrounded by degranulated eosinophils.
{"title":"Equine cutaneous habronematidosis","authors":"F. Navolar, Julio Kioquetta, M. Ribeiro, A. Dearo, T. Faccin","doi":"10.24070/bjvp.1983-0246.v15i2p121-123","DOIUrl":"https://doi.org/10.24070/bjvp.1983-0246.v15i2p121-123","url":null,"abstract":"Clinical history: A 4-year-old Quarter Horse stallion was unsuccessfully treated for a circumscribed and ulcerated lesion of unknown duration located lateral to the prepuce. Microscopic description: The superficial and deep preputial dermis contains multifocal to coalescing, well-demarcated eosinophilic granulomas characterized by a central area of abundant eosinophilic necrotic debris mixed with degenerate eosinophils surrounded by numerous epithelioid macrophages and occasional multinucleated giant cells (foreign-body type), rimmed by ill-defined layers of fibrous connective tissue. In the center of the granulomas, there are cross-sections of degenerate nematode larvae. The adjacent dermal collagen is expanded by dense inflammatory infiltrate composed mainly of eosinophils and a few lymphocytes and plasma cells. The dermal microvasculature is prominent, with hypertrophic reactive endothelial lining. There is also vascular proliferation amidst perpendicularly oriented plump fibroblasts embedded in collagen (granulation tissue). In addition, there are numerous shrunken, hypereosinophilic, fragmented or hyalinized collagen bundles, occasionally surrounded by degranulated eosinophils.","PeriodicalId":9223,"journal":{"name":"Brazilian Journal of Veterinary Pathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43830339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-30DOI: 10.24070/bjvp.1983-0246.v15i2p93-98
Verónica de Oca, S. Martínez, E. Salinas, R. Olivares, F. Sánchez-Godoy
Aspergillosis, the main causative agent of which is Aspergillus fumigatus, causes mortality in all types of birds. Gliotoxin (GT), one of the multiple virulence factors of A. fumigatus, has a variety of immunosuppressive effects. The corpse of an African grey parrot (Psittacus erithacus) was sent for necropsy and diagnostic rule-out. The lungs were enlarged, firm, and had dark-red coloration, on the parietal faces of both lungs, some semi-circular caseous necrosis areas were observed. The caudal thoracic and abdominal air sacs were thickened and contained a fibrin-heterophilic exudate. Histopathologically, a necrotic and granulomatous bronchopneumonia was observed with intralesional hyphae with characteristics compatible with Aspergillus sp. that were positive with Grocott´s staining. Fibrinous and heterophilic airsacculitis was found in the air sacs. A. fumigatus was isolated from lungs, characterized using serial microcultures, and confirmed using polymerase chain reaction. In addition, GT production was detected in vitro from the culture filtrate in which the isolate was grown; the organic extract was analysed via thin-layer chromatography. This is the first detection of GT in a case of pulmonary aspergillosis in a parrot, which could help to understand the pathogenesis of the disease in psittacines.
{"title":"Pathological and mycological characterization of pulmonary Aspergillus fumigatus infection producing gliotoxin in a captive African grey parrot (Psittacus erithacus)","authors":"Verónica de Oca, S. Martínez, E. Salinas, R. Olivares, F. Sánchez-Godoy","doi":"10.24070/bjvp.1983-0246.v15i2p93-98","DOIUrl":"https://doi.org/10.24070/bjvp.1983-0246.v15i2p93-98","url":null,"abstract":"Aspergillosis, the main causative agent of which is Aspergillus fumigatus, causes mortality in all types of birds. Gliotoxin (GT), one of the multiple virulence factors of A. fumigatus, has a variety of immunosuppressive effects. The corpse of an African grey parrot (Psittacus erithacus) was sent for necropsy and diagnostic rule-out. The lungs were enlarged, firm, and had dark-red coloration, on the parietal faces of both lungs, some semi-circular caseous necrosis areas were observed. The caudal thoracic and abdominal air sacs were thickened and contained a fibrin-heterophilic exudate. Histopathologically, a necrotic and granulomatous bronchopneumonia was observed with intralesional hyphae with characteristics compatible with Aspergillus sp. that were positive with Grocott´s staining. Fibrinous and heterophilic airsacculitis was found in the air sacs. A. fumigatus was isolated from lungs, characterized using serial microcultures, and confirmed using polymerase chain reaction. In addition, GT production was detected in vitro from the culture filtrate in which the isolate was grown; the organic extract was analysed via thin-layer chromatography. This is the first detection of GT in a case of pulmonary aspergillosis in a parrot, which could help to understand the pathogenesis of the disease in psittacines.","PeriodicalId":9223,"journal":{"name":"Brazilian Journal of Veterinary Pathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48535099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-30DOI: 10.24070/bjvp.1983-0246.v15i2p99-104
Mario Balario, F. Gonçalves, F. Leal, I. Cosentino, J. Vignoli, Nathalia Silvia, Felipe Brandão, A. Nassar, S. Miyashiro, N. Cunha, C. Del Fava
We described an outbreak of abomasal bloat in goat kids. Increased mortality with a history of abdominal bloating, dullness, and death was reported. Postmortem exanimation revealed dehydration, pale mucosa, ascites, abomasal tympanism and intestinal meteorism and congestion, and emphysematous abomasitis. Cytological evaluation from abomasum revealed gram-positive bacteria with a cuboid shape suggestive of Clostridium ventriculi, gram-positive bacilli suggestive of Clostridium perfringens, and ovoid basophilic yeasts. C. ventriculi and C. perfringens type A were confirmed by anaerobic culture and molecular tests. Histopathological findings revealed emphysematous abomasitis, necrosis of the gastric and intestinal walls, gastroenteritis, and intestinal thromboembolism. The possible associated factor was erroneous use of the milk replacer associated with inadequate kid management. Finally, the prophylactic measures suggested such as hygiene care, proper use of milk replacer, clostridial vaccination plan, and a good colostrum management were able to control the outbreak.
{"title":"Outbreak of abomasal bloat in goat kidsdue to Clostridium ventriculi and Clostridium perfringens type A","authors":"Mario Balario, F. Gonçalves, F. Leal, I. Cosentino, J. Vignoli, Nathalia Silvia, Felipe Brandão, A. Nassar, S. Miyashiro, N. Cunha, C. Del Fava","doi":"10.24070/bjvp.1983-0246.v15i2p99-104","DOIUrl":"https://doi.org/10.24070/bjvp.1983-0246.v15i2p99-104","url":null,"abstract":"We described an outbreak of abomasal bloat in goat kids. Increased mortality with a history of abdominal bloating, dullness, and death was reported. Postmortem exanimation revealed dehydration, pale mucosa, ascites, abomasal tympanism and intestinal meteorism and congestion, and emphysematous abomasitis. Cytological evaluation from abomasum revealed gram-positive bacteria with a cuboid shape suggestive of Clostridium ventriculi, gram-positive bacilli suggestive of Clostridium perfringens, and ovoid basophilic yeasts. C. ventriculi and C. perfringens type A were confirmed by anaerobic culture and molecular tests. Histopathological findings revealed emphysematous abomasitis, necrosis of the gastric and intestinal walls, gastroenteritis, and intestinal thromboembolism. The possible associated factor was erroneous use of the milk replacer associated with inadequate kid management. Finally, the prophylactic measures suggested such as hygiene care, proper use of milk replacer, clostridial vaccination plan, and a good colostrum management were able to control the outbreak.","PeriodicalId":9223,"journal":{"name":"Brazilian Journal of Veterinary Pathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43604561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-30DOI: 10.24070/bjvp.1983-0246.v15i2p114-117
Anne Ching-Nga Tse, Carlton Pak-Man Yuen
Clinical History: Five-year-old, spayed, stray brown cow with generalized cutaneous nodules present for a week. Necropsy Findings: The animal is in good nutritional condition. Several ticks are present on the ventrum and withers. Multifocally and widespread, affecting all areas of skin in a random distribution, there are variably sized (1-4 cm in diameter) cutaneous and/or subcutaneous nodules, sometimes with a targetoid appearance. A few nodules are ulcerated, and others have a central dense crust. On cut section, many nodules are well demarcated, with a light pink center delineated by a dark red haemorrhagic/hyperaemicline (acute infarcts). Similar nodules and ulcers are also present over the teats. Skinning reveals extensive subcutaneous thickening with yellowish gelatinous material (oedema) as well as multifocal areas of dark red discoloration (haemorrhages). Multiple superficial lymph nodes are enlarged and haemorrhagic, with the right pre-scapular lymph node most severely affected, measuring 16x7x8 cm.
{"title":"Lumpy skin disease in a cow","authors":"Anne Ching-Nga Tse, Carlton Pak-Man Yuen","doi":"10.24070/bjvp.1983-0246.v15i2p114-117","DOIUrl":"https://doi.org/10.24070/bjvp.1983-0246.v15i2p114-117","url":null,"abstract":"Clinical History: Five-year-old, spayed, stray brown cow with generalized cutaneous nodules present for a week. Necropsy Findings: The animal is in good nutritional condition. Several ticks are present on the ventrum and withers. Multifocally and widespread, affecting all areas of skin in a random distribution, there are variably sized (1-4 cm in diameter) cutaneous and/or subcutaneous nodules, sometimes with a targetoid appearance. A few nodules are ulcerated, and others have a central dense crust. On cut section, many nodules are well demarcated, with a light pink center delineated by a dark red haemorrhagic/hyperaemicline (acute infarcts). Similar nodules and ulcers are also present over the teats. Skinning reveals extensive subcutaneous thickening with yellowish gelatinous material (oedema) as well as multifocal areas of dark red discoloration (haemorrhages). Multiple superficial lymph nodes are enlarged and haemorrhagic, with the right pre-scapular lymph node most severely affected, measuring 16x7x8 cm.","PeriodicalId":9223,"journal":{"name":"Brazilian Journal of Veterinary Pathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42445082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-31DOI: 10.24070/bjvp.1983-0246.v15i1p57-61
Nicolás Enríquez Montenegro, Diana A. Ríos Castro, Paola A. Bedoya López, Carlos A. Chaves Velásquez, Tatiana G. Paz Calvache
A female Persian cat arrives for clinical assessment with a 3-month history of weight loss and sporadic vomiting. The clinical and paraclinical findings were hypodynamia cachexia, leukocytosis and presence of a mass in duodenum. Histopathological evaluation revealed a non-neoplastic tumor proliferation, which was organized into dense, sclerotic-like connective tissue trabeculae that anastomosed, with cells of spindle-shaped morphology, elongated and rounded nuclei with prominent nucleoli and fine granular chromatin. These cells were intermingled with abundant eosinophils and in smaller proportion lymphocytes, macrophages, plasma cells, with transmural distribution. Masson's trichrome differential staining trabeculae of collagen fibers. Based on the clinical and microscopic findings, the diagnosis of feline eosinophilic sclerosing fibroplasia is established, being this pathology’s first documented report in Colombia.
{"title":"Feline gastrointestinal eosinophilic sclerosing fibroplasia","authors":"Nicolás Enríquez Montenegro, Diana A. Ríos Castro, Paola A. Bedoya López, Carlos A. Chaves Velásquez, Tatiana G. Paz Calvache","doi":"10.24070/bjvp.1983-0246.v15i1p57-61","DOIUrl":"https://doi.org/10.24070/bjvp.1983-0246.v15i1p57-61","url":null,"abstract":"A female Persian cat arrives for clinical assessment with a 3-month history of weight loss and sporadic vomiting. The clinical and paraclinical findings were hypodynamia cachexia, leukocytosis and presence of a mass in duodenum. Histopathological evaluation revealed a non-neoplastic tumor proliferation, which was organized into dense, sclerotic-like connective tissue trabeculae that anastomosed, with cells of spindle-shaped morphology, elongated and rounded nuclei with prominent nucleoli and fine granular chromatin. These cells were intermingled with abundant eosinophils and in smaller proportion lymphocytes, macrophages, plasma cells, with transmural distribution. Masson's trichrome differential staining trabeculae of collagen fibers. Based on the clinical and microscopic findings, the diagnosis of feline eosinophilic sclerosing fibroplasia is established, being this pathology’s first documented report in Colombia.","PeriodicalId":9223,"journal":{"name":"Brazilian Journal of Veterinary Pathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47427085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-31DOI: 10.24070/bjvp.1983-0246.v15i1p31-37
T. D. de Carvalho, C. Eckstein, Larissa L. de Moura, N. Heleno, L. D. da Silva, D. D. Dos Santos, L. D. de Souza, A. R. Oliveira, R. Xavier, M. Thompson, R. Silva, R. Santos
This report describes a case of a pyogranulomatous dermatitis, osteomyelitis, and meningitis with Splendore-Hoeppli reaction caused by Staphylococcus aureus in a sheltered female adult cat coinfected with the feline leukemia virus (FeLV) and Leishmania sp. The cat had a mild anemia and marked increased total leukocytes, particularly band and segmented neutrophils. The cat had laboratorial diagnosis of FeLV and Leishmania sp. infections. Clinically, the cat had extensive and multifocal areas of ulceration in cranial region. Due to the progression of cutaneous lesions, progressive weight loss, and the risk for other sheltered animals, the cat was euthanized. Microscopically, there was marked pyogranulomatous ulcerative dermatitis, osteomyelitis and meningitis, with multiple large intralesional colonies of Gram-positive cocci associated with Splendore-Hoeppli reaction. Aerobic bacterial isolates were identified as S. aureus by MALDI-ToF MS. Leishmania sp. DNA sequences were detected in liver and spleen, and amastigotes were demonstrated in skin sections by immunohistochemistry. In conclusion, here we describe a case of S. aureus-induced pyogranulomatous meningitis with SH reaction in a cat naturally coinfected with FeLV and Leishmania sp.
{"title":"Staphylococcus aureus-induced pyogranulomatous dermatitis, osteomyelitis, and meningitis with Splendore-Hoeppli reaction in a cat coinfected with the feline leukemia virus and Leishmania sp.","authors":"T. D. de Carvalho, C. Eckstein, Larissa L. de Moura, N. Heleno, L. D. da Silva, D. D. Dos Santos, L. D. de Souza, A. R. Oliveira, R. Xavier, M. Thompson, R. Silva, R. Santos","doi":"10.24070/bjvp.1983-0246.v15i1p31-37","DOIUrl":"https://doi.org/10.24070/bjvp.1983-0246.v15i1p31-37","url":null,"abstract":"This report describes a case of a pyogranulomatous dermatitis, osteomyelitis, and meningitis with Splendore-Hoeppli reaction caused by Staphylococcus aureus in a sheltered female adult cat coinfected with the feline leukemia virus (FeLV) and Leishmania sp. The cat had a mild anemia and marked increased total leukocytes, particularly band and segmented neutrophils. The cat had laboratorial diagnosis of FeLV and Leishmania sp. infections. Clinically, the cat had extensive and multifocal areas of ulceration in cranial region. Due to the progression of cutaneous lesions, progressive weight loss, and the risk for other sheltered animals, the cat was euthanized. Microscopically, there was marked pyogranulomatous ulcerative dermatitis, osteomyelitis and meningitis, with multiple large intralesional colonies of Gram-positive cocci associated with Splendore-Hoeppli reaction. Aerobic bacterial isolates were identified as S. aureus by MALDI-ToF MS. Leishmania sp. DNA sequences were detected in liver and spleen, and amastigotes were demonstrated in skin sections by immunohistochemistry. In conclusion, here we describe a case of S. aureus-induced pyogranulomatous meningitis with SH reaction in a cat naturally coinfected with FeLV and Leishmania sp.","PeriodicalId":9223,"journal":{"name":"Brazilian Journal of Veterinary Pathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46320943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-31DOI: 10.24070/bjvp.1983-0246.v15i1p38-43
R. M. Pereira, J. L. de Araujo, Márcio A. Silva, G. B. S. Filho, H. A. Bom, L. D. Aires, F. A. Souza, F. Mendonça
A captive 17-year-old male crab-eating fox (Cerdocyon thous) presenting clinical signs of hyporexia and constipation for two weeks was found dead. On autopsy, a large and firm mass expanding the large intestine wall and totally obstructing the colon lumen was identified. Histopathology revealed a non-encapsulated poorly demarcated adenocarcinoma composed of cuboidal to pseudostratified columnar epithelium forming papillary projections of variable sizes. The cytoplasm of the neoplastic cells was immunopositive for CK7 and PAN-CK, but negative for VIM and CK20. Immunohistochemistry revealed an immunophenotype different from the enteric adenocarcinomas reported in humans and domestic dogs. This is the first case report of colonic papillary adenocarcinoma in a crab-eating fox.
{"title":"Colonic papillary adenocarcinoma in a crab-eating fox (Cerdocyon thous): Anatomopathological and immunohistochemical features","authors":"R. M. Pereira, J. L. de Araujo, Márcio A. Silva, G. B. S. Filho, H. A. Bom, L. D. Aires, F. A. Souza, F. Mendonça","doi":"10.24070/bjvp.1983-0246.v15i1p38-43","DOIUrl":"https://doi.org/10.24070/bjvp.1983-0246.v15i1p38-43","url":null,"abstract":"A captive 17-year-old male crab-eating fox (Cerdocyon thous) presenting clinical signs of hyporexia and constipation for two weeks was found dead. On autopsy, a large and firm mass expanding the large intestine wall and totally obstructing the colon lumen was identified. Histopathology revealed a non-encapsulated poorly demarcated adenocarcinoma composed of cuboidal to pseudostratified columnar epithelium forming papillary projections of variable sizes. The cytoplasm of the neoplastic cells was immunopositive for CK7 and PAN-CK, but negative for VIM and CK20. Immunohistochemistry revealed an immunophenotype different from the enteric adenocarcinomas reported in humans and domestic dogs. This is the first case report of colonic papillary adenocarcinoma in a crab-eating fox.","PeriodicalId":9223,"journal":{"name":"Brazilian Journal of Veterinary Pathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42447612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-31DOI: 10.24070/bjvp.1983-0246.v15i1p11-19
R. A. Pereira, A. F. Alves, I. D. de Amorim, Igor de Campos Fontes, Gabriela A. Santos, L. S. Campos, R. V. Nunes, H. Andrade, É. R. de Alvarenga, W. Tafuri
The aim of this work was to describe the anatomical pathology of dogs experimentally infected with Leishmania (Leishmania) infantum strain MCAN / BR / 2002 / BH401, a Brazilian form of L. infantum isolated from a symptomatic dog from an endemic area. For this, five beagles (three months old and both sexes) composed the experimental group. Markers of macrophage subpopulations M1 and M2 (related to resistance and susceptibility to visceral leishmaniasis) and the tissue cytokine transforming growth factor beta 1 (TGF-β1) (one of the main cytokines related to the fibrosis process and anti-inflammatory action) were evaluated in livers, lungs and kidneys. The BH 401 L. infantum strain induced classical lesions of the visceral disease where all evaluated organs showed a chronic inflammatory reaction and tissue parasitism associated with a higher expression of CD163 and TGF-β1 markers, might be related to the progression of the disease. In this work it was possible to conclude that the BH 401 strain reproduces canine visceral leishmaniasis that occurs naturally.
{"title":"Characterization of macrophage polarization in lesions of dogs inoculated with Leishmania (Leishmania) infantum (BH40) strain","authors":"R. A. Pereira, A. F. Alves, I. D. de Amorim, Igor de Campos Fontes, Gabriela A. Santos, L. S. Campos, R. V. Nunes, H. Andrade, É. R. de Alvarenga, W. Tafuri","doi":"10.24070/bjvp.1983-0246.v15i1p11-19","DOIUrl":"https://doi.org/10.24070/bjvp.1983-0246.v15i1p11-19","url":null,"abstract":"The aim of this work was to describe the anatomical pathology of dogs experimentally infected with Leishmania (Leishmania) infantum strain MCAN / BR / 2002 / BH401, a Brazilian form of L. infantum isolated from a symptomatic dog from an endemic area. For this, five beagles (three months old and both sexes) composed the experimental group. Markers of macrophage subpopulations M1 and M2 (related to resistance and susceptibility to visceral leishmaniasis) and the tissue cytokine transforming growth factor beta 1 (TGF-β1) (one of the main cytokines related to the fibrosis process and anti-inflammatory action) were evaluated in livers, lungs and kidneys. The BH 401 L. infantum strain induced classical lesions of the visceral disease where all evaluated organs showed a chronic inflammatory reaction and tissue parasitism associated with a higher expression of CD163 and TGF-β1 markers, might be related to the progression of the disease. In this work it was possible to conclude that the BH 401 strain reproduces canine visceral leishmaniasis that occurs naturally.","PeriodicalId":9223,"journal":{"name":"Brazilian Journal of Veterinary Pathology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42456011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-31DOI: 10.24070/bjvp.1983-0246.v15i1p44-49
A. Consalter, J. Guerra, J. Leite, R. Réssio, J. Barbosa, A. Ferreira, M. Balaro
An 11-year-old pet goat presented a clinical history of acute respiratory distress with ultrasound diagnosis of wide lung injury. The animal was euthanized due to welfare reasons. At necropsy, it was found pleural effusion and adhesion on the right cranioventral thoracic region. The right cranial and middle lung lobes were firm and light gray with a neocavity containing purulent exudate. From the middle lobe, there was a nodular proliferation occupying alveolar spaces, densely cellular and composed by cuboid-columnar epithelial cells arranged in papillae and acini (60%), as well as polygonal cells arranged in nests with squamous differentiation (40%). Marked pleomorphism, anisocytosis and anisocariosis were also noted. A total of 39 mitosis figures for ten fields at 400x magnification were counted. Lung samples were negative for jaagsiekte retrovirus (JSRV) by PCR. Immunostaining for TTF1 and P53 occurred in zones of adenomatous and squamous differentiation, respectively. In MIB-1, 14% (82/594) of immunolabeled cells were observed in the squamous component. In conclusion, the histopathological and immunohistochemical characteristics confirmed the diagnosis of a pulmonary adenosquamous carcinoma, without JSRV involvement, in goat species.
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