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Bioconversion of agricultural and industrialised waste to generate valuable products 农业和工业废物的生物转化,以产生有价值的产品
Pub Date : 2019-12-31 DOI: 10.21472/bjbs.061411
Daniel Elijah Ngbede, U. Ahmadu, M. Gana, F. Attah
Agro and industrial wastes are rich in bioactive compounds. These wastes can be used as an alternate source for the production of different valuable products as the raw material in various industries. The use of agro-industrial wastes as raw materials can help to reduce the production cost and also reduce the pollution load from the environment. Agro-industrial wastes are used for manufacturing of enzymes, biofertilizer, biofuel, antibiotics, and other chemicals through solid state fermentation (SSF). A variety of microorganisms are used for the production of these valuable products through SSF processes. This reviewed work was aimed at bioconversion of agricultural and industrial wastes to generate valuable products.
农业和工业废物中含有丰富的生物活性化合物。这些废物可以作为生产不同有价值产品的替代来源,作为各种工业的原材料。利用农业工业废料作为原料可以帮助降低生产成本,也可以减少来自环境的污染负荷。农业工业废物通过固态发酵(SSF)用于制造酶、生物肥料、生物燃料、抗生素和其他化学品。各种微生物被用于通过SSF工艺生产这些有价值的产品。本文综述了农业和工业废物的生物转化,以产生有价值的产品。
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引用次数: 0
The use of functionality scales in patients with Post-Polio Syndrome 脊髓灰质炎后综合征患者功能量表的使用
Pub Date : 2019-08-31 DOI: 10.21472/bjbs.061305
Aline Simão Helou, A. Ramos, A. Oliveira, A. Quadros, F. Fávero
Post-Polio Syndrome (PPS) is a neurological disease, resulting in the decrease of the neuromuscular functions being characterized by a set of clinical manifestations that occur in individuals who had acute paralytic poliomyelitis. The patients with PPS begin to present new limitations on the performance of their ADLs and, at the same time, tend to ignore them, continuing their daily work with greater physical effort. The need for a functional evaluation is therefore necessary to find out where the commitments are in the ADLs and to help them without aggravating the degenerative PPS. To identify the main functional assessment scales used in individuals with PPS. A study review of the literature was performed on the functional scales used in patients with PPS described in the articles in the database. We found 12 functional scales in activities of daily living, as follows: Nottingham Health Profile (NHP), International Classification of Impairments, Disability's and Handicaps (ICIDH), Functional Status Questionnaire (FSQ), Physical Activity Scale for the Elderly (PASE), Sunnas ADL Index, Katz Index, Falls Efficacy Scale (FES), ADLs-Staircase, Barthel Index, Functional Independence Measure (FIM), Older Adult Resource Survey (OARS), and Self-Reporter ADL. None of the 12 scales mentioned in our study is validated for the use in patients with PPS, the most scales found in the literatures reviewed in this research were NHP and ICIDH. Therefore, we found 9 scales validated in Brazil, Barthel Index, Functional Independence Measure (FIM), Functional Status Questionnaire (FSQ), Falls Efficacy Scale (FES), Nottingham Health Profile (NHP), International Classification of Impairments, Disability's and Handicaps (ICIDH), Older Adult Resource Survey (OARS), Katz Index and Self-Reporter ADL.
脊髓灰质炎后综合征(PPS)是一种神经系统疾病,导致神经肌肉功能下降,其特征是急性麻痹性脊髓灰质炎患者出现的一系列临床表现。患有PPS的患者开始对其adl的表现提出新的限制,同时倾向于忽略它们,以更大的体力继续他们的日常工作。因此,有必要进行功能评价,以查明发展中国家的承诺在哪里,并在不加重退行性PPS的情况下帮助他们。确定PPS患者的主要功能评估量表。对数据库中文章中描述的PPS患者使用的功能量表进行了文献研究综述。我们发现了12种日常生活活动功能量表,分别是:Nottingham Health Profile (NHP)、International Classification of impairment, Disability's and handicapped (ICIDH)、functional Status Questionnaire (FSQ)、Physical Activity Scale for Elderly (PASE)、Sunnas ADL Index、Katz Index、Falls Efficacy Scale (FES)、ADLs-Staircase、Barthel Index、functional Independence Measure (FIM)、Elderly Adult Resource Survey (OARS)、Self-Reporter ADL。我们研究中提到的12种量表均未被证实可用于PPS患者,本研究查阅的文献中发现的量表最多的是NHP和ICIDH。因此,我们找到了巴西验证的9种量表:Barthel指数、功能独立性量表(FIM)、功能状态问卷(FSQ)、跌倒效果量表(FES)、诺丁汉健康状况量表(NHP)、国际损伤、残疾和残障分类量表(ICIDH)、老年人资源调查量表(OARS)、Katz指数和自我报告ADL。
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引用次数: 0
Antisnake venom activity and isolation of quercetin from the leaf of Neocarya macrophylla (Sabine) Prance ex F. White (Malpighiales: Chrysobalanaceae) 巨叶新果(Neocarya macrophylla (Sabine) Prance ex F. White)抗蛇毒活性及槲皮素的分离
Pub Date : 2019-08-31 DOI: 10.21472/bjbs.061306
A. Yusuf, M. Abdullahi, A. Musa, A. Haruna, V. Mzozoyana, H. Abubakar
Snake envenomation is a major cause of death and disability in many developing countries. Neocarya macrophylla (Sabine) Prance ex F. White (Malpighiales: Chrysobalanaceae) have been reportedly used in traditional medicine to treat snake envenomation. Bioassay-guided isolation of antivenom principles was carried out on the leaf of N. macrophylla against Naja nigricollis venom. The methanol extract of N. macrophylla leaf and its ethylacetate and n-butanol fraction significantly (P < 0.05) protected mice against venom-induced lethality with 100% survival rate and there was remarkable inhibition of the poisonous effects of PLA2 enzyme by the extracts and the fractions. Encouraged by this result, the ethylacetate soluble fraction was subjected to purification using vacuum liquid chromatography and gel filtration which led to the isolation of quercetin as the bioactive principle. The identity of the compound was determined on the basis of chemical tests, and by comparison of its 1H-NMR data with literature, this is the first report of isolation of this compound from the leaf of the plant. However, the results of the study suggests that the leaf of N. macrophylla possess significant antisnake venom activity which provide the scientific basis for its use in traditional treatment of snakebites.
在许多发展中国家,蛇中毒是造成死亡和残疾的主要原因。据报道,大叶新树(Sabine) Prance ex F. White (Malpighiales: Chrysobalanaceae)已在传统医学中用于治疗蛇中毒。获得抗蛇毒血清原则进行了隔离的叶n macrophylla对眼镜蛇nigricollis毒液。大叶青叶甲醇提取物及其乙酸乙酯和正丁醇部位对小鼠的毒致死亡有显著的保护作用(P < 0.05),小鼠存活率为100%,对PLA2酶的毒作用有显著的抑制作用。受此结果的鼓舞,对乙酸乙酯可溶性部分进行了真空液相色谱和凝胶过滤的纯化,从而分离出槲皮素作为生物活性原理。该化合物的身份是在化学测试的基础上确定的,并通过其1H-NMR数据与文献进行比较,这是从植物叶片中分离出该化合物的第一份报告。研究结果表明,大叶青叶具有明显的抗蛇毒活性,为其在传统蛇咬伤治疗中的应用提供了科学依据。
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引用次数: 9
Effect of administration of neurotransmitters: Serotonin and dopamine on testicular maturation in the freshwater crab Travancoriana schirnerae Bott, 1969 (Crustacea: Gecarcinucidae) 神经递质:血清素和多巴胺对淡水蟹睾丸成熟的影响,1969(甲壳纲:蟹科)
Pub Date : 2019-08-31 DOI: 10.21472/bjbs.061309
N. Gopal, A. Devi
The current investigation aimed at the effect of administration of the neurotransmitters, serotonin and dopamine, on testicular maturation in the freshwater crab Travancoriana schirnerae Bott, 1969 (Crustacea: Gecarcinucidae). Adult males in active, inactive and revival phases of spermatogenesis were injected with serotonin and dopamine in multiple doses and their dissected testes were submitted to histomorphological examinations. Though serotonin treatment during active phase showed increased values for testicular index, acinar diameter and proportion of mature spermatozoa, only the testicular index values differed statistically from that of controls. Serotonin administration during inactive phase caused an increase in testicular index and acinar diameter, reduction in intra and interacinar spaces and pycnosis of germ cells and detection of division stages. A notable increase in the testicular index, acinar diameter and fresh batches of spermatozoa was perceptible in crabs injected with serotonin during revival phase. In contrast, dopamine injection showed a decline in testicular activity irrespective of the phase of spermatogenesis, as evinced from low testicular index, acinar diameter, presence of small, irregularly shaped acini with indistinct acinar boundaries, reduction in the number of mature spermatozoa and absence of division stages. To conclude, the stimulatory neurotransmittant, serotonin can be used to induce testicular maturation in species of aquaculture potential.
本研究旨在研究神经递质、血清素和多巴胺对淡水蟹trancorana schirnerae Bott睾丸成熟的影响,1969(甲壳纲:gecocarcinidae)。对处于精子发生活跃期、不活跃期和恢复期的成年雄性分别注射多剂量血清素和多巴胺,解剖睾丸后进行组织形态学检查。活动期血清素治疗可提高睾丸指数、腺泡直径和成熟精子比例,但只有睾丸指数与对照组有统计学差异。在非活跃期给药血清素导致睾丸指数和腺泡直径增加,腺泡内和腺泡间间隙减小,生殖细胞增生,分裂期检测。在恢复期注射5 -羟色胺的螃蟹睾丸指数、腺泡直径和新鲜精子数量显著增加。相比之下,多巴胺注射显示睾丸活性下降,与精子发生的阶段无关,睾丸指数低,腺泡直径低,存在小而不规则形状的腺泡,腺泡边界不清,成熟精子数量减少,没有分裂阶段。综上所述,刺激性神经递质血清素可用于诱导有养殖潜力的种鱼睾丸成熟。
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引用次数: 0
Arbuscular mycorrhizal association and dependency determined the pioneer plant community, diversity structure and plant cover in two river bank under same climate 丛枝菌根的结合力和依赖性决定了同一气候条件下两河流域先锋植物群落、多样性结构和植被覆盖
Pub Date : 2019-08-31 DOI: 10.21472/bjbs.061313
Somdatta Ghosh, D. Kuila, N. Verma
Early colonization of plants in an area is influenced mostly by climatic, edaphic and phytogeographic factors. As arbuscular mycorrhizae (AM) was associated with early invasion of land plants on earth, AM may have some role in defining the first seral community in any land. Two riverbanks were selected to study their pioneer plant community structure with species composition and diversity, soil characters and arbuscular mycorrhizal association; and correlations among these factors. Species composition, diversity and richness indices, active AM association of early colonizing plant species, soil texture, moisture, pH and E.C in two river banks differed. Similarity index for plant species between the two communities was poor. Diversity and richness indices were high in K site while evenness was high in R site. AM colonization and spore density correlated highly with plant cover and frequency in both riverbanks. Soil moisture showed a strong negative impact on mycorrhization, soil organic carbon showed little. Soil pH showed varied correlation in different sites. Early colonizing plants in R site with silt-loam soil with high moisture level are found poorly mycotrophic or nonmycotrophic; though plant cover correlated highly with mycotrophy in both sites. Plants in sandy soil of K site are highly mycotrophic and with high arbuscular and vesicular colonizations. The distribution of frequency in R site is highly deviated from Raunkiuer’s frequency class; in K site it is rather stable. The soil condition is only key factor to determine plant composition and plant-mycorrhizal relations influencing colonization of early seral community.
植物在一个地区的早期定植主要受气候、土壤和植物地理因素的影响。丛枝菌根(AM)与陆地植物的早期入侵有关,它可能在确定陆地上最早的几个群落中起一定的作用。选取2条河堤,研究其先锋植物群落结构、物种组成和多样性、土壤特征及丛枝菌根关联;以及这些因素之间的相关性。两河岸的物种组成、多样性和丰富度指数、早期定殖植物的AM活性关联、土壤质地、湿度、pH和E.C均存在差异。群落间植物种类相似性指数较低。多样性和丰富度指数在K位点较高,均匀度指数在R位点较高。AM定植和孢子密度与两岸植被覆盖度和频率高度相关。土壤湿度对菌根的负向影响较强,土壤有机碳对菌根的负向影响较小。土壤pH值在不同立地表现出不同的相关性。高水分粉壤土R点早期定殖植物的真菌营养不良或无真菌营养;植被覆盖与真菌侵染呈高度相关。K点沙质土壤的植物是高度真菌营养的,具有较高的丛枝和水疱定植。R点的频率分布与Raunkiuer频率分类偏差较大;在K位点它是相当稳定的。土壤条件是决定植物组成和影响早期几种群落定植的植物-菌根关系的唯一关键因素。
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引用次数: 0
Nutritional, haematinic and biosafety evaluation of Ipomoea batatas (L.) Lam. (Solanales: Convolvulaceae) leaf extract on albino rats 马铃薯的营养、血液学和生物安全性评价林。(茄属植物:旋花科)叶提取物对白化大鼠的作用
Pub Date : 2019-08-31 DOI: 10.21472/BJBS.061301
B. Gabriel, M. Idu
This study evaluates the nutritional, Haematinic and biosafety of Ipomoea batatas (L.) Lam. (Solanales: Convolvulaceae) aqueous leaf extract on albino rats. Proximate and mineral compositions were determined using standard methods. Haematinic activity of the plant was done using graded doses; 125, 250, and 500 mg/kg and 40 mg/kg phenyl hydrazine hydrochloride to induce anaemia. A modified method was used for acute and sub-acute toxicological evaluations. Results from the study showed that aqueous extracts of I. batatas had significant increase in RBC, HCT, Hgb, MCV, MCH and MCHC at 7.19+/-0.45, 46.13+/-0.08, 13.43+/-0.27, 82.40+/-0.92, 24.15+/-1.24 and 37.78+/-0.20, respectively, when compared with the control group. Acute study showed no pathological behaviour with absent mortality. Sub-acute study of the spleen, heart, liver and kidney showed a mild activation of local immune system. The extracts recorded 89.14% scavenging property compared with 94.3% ascobate in antioxidant study. Proximate analysis had 31.56% of moisture content, 16.25% of protein, 7.64% of ash, 0.37% of crude fibre, 0.19% of fat and 43.99% of carbohydrate. Investigation of calcium, magnesium, Iron, potassium, phosphorus, sodium and zinc were 28.03, 339.61, 15.87, 4.61, 35.90, 4.21 and 0.08 mg/kg others were not detected. This may be due to stimulating mechanism of Myelo-Erythroid cell ratio in bone marrow or antioxidant effect. Result thus validates ethno-botanical uses of I. batatas for the treatment of anaemia.
本研究评价了马铃薯的营养、血化学和生物安全性。林。(茄属植物:旋花科)水提取物对白化大鼠的作用。比邻物和矿物成分采用标准方法测定。用分级剂量测定植物的血碱活性;125、250、500mg /kg和40mg /kg盐酸苯肼诱导贫血。采用改良的方法进行急性和亚急性毒理学评价。结果表明,与对照组相比,红血球水提液红细胞、HCT、Hgb、MCV、MCH和MCHC分别显著升高7.19+/-0.45、46.13+/-0.08、13.43+/-0.27、82.40+/-0.92、24.15+/-1.24和37.78+/-0.20。急性期未见病理性行为,无死亡。脾脏、心脏、肝脏和肾脏的亚急性研究显示局部免疫系统轻度激活。在抗氧化研究中,与抗坏血酸94.3%相比,提取物的清除率达到89.14%。水分含量31.56%,蛋白质含量16.25%,灰分含量7.64%,粗纤维含量0.37%,脂肪含量0.19%,碳水化合物含量43.99%。钙、镁、铁、钾、磷、钠、锌的检出值分别为28.03、339.61、15.87、4.61、35.90、4.21和0.08 mg/kg,其余未检出。这可能与骨髓中髓红细胞比例的刺激机制或抗氧化作用有关。从而验证了巴塔塔在治疗贫血方面的民族植物学应用。
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引用次数: 0
Digenean parasites of the Family Lecithodendriidae (Luhe, 1901) Odhner, 1901 infecting the frog Hoplobatrachus tigerinus (Anura: Dicroglossidae) of the Western Ghats, Wayanad Region, India 印度瓦亚纳德地区西高止山脉虎蛙(无尾目:虎蛙科)感染的蝗科寄生虫(Luhe, 1901
Pub Date : 2019-08-31 DOI: 10.21472/bjbs.061315
Dr. Shinad K, P. K. Prasadan
Three species of digenean parasites, Pleurogenoides euphlycti, P. wayanadensis and Meharorchis ranarum of the frog Hoplobatrachus tigerinus (Anura, Dicroglossidae) from the Wayanad Region of the South Western Ghats are reported in this paper. Multiple infections were also recorded during the study. Prevalence of infection of P. euphlycti, P. wayanadensis and M. ranarum were 15.15%, 9.09% and 12.12%, respectively, and the intensity of infection were 5.2, 7.3 and 8, and the mean abundance were 0.78, 0.66 and 0.96, respectively. Mean abundance is an indication of the dispersion of parasites among hosts. The mean abundance and the intensity were at the maximum level in M. ranarum infection and that of prevalence of infection was at the maximum level in of P. euphlycti infection.
本文报道了西南高止山脉瓦亚纳德地区虎尾蛙(无尾目,双翅蛙科)的三种地沟寄生虫pleurogenides euphlycti、P. wayanadensis和Meharorchis ranarum。研究期间还记录了多次感染。胡杨假丝兰、瓦氏假丝兰和ranarum的感染率分别为15.15%、9.09%和12.12%,感染强度分别为5.2、7.3和8,平均丰度分别为0.78、0.66和0.96。平均丰度是寄生虫在宿主之间分散的一个指标。ranarum感染的平均丰度和强度最高,而phlylycti感染的平均感染率最高。
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引用次数: 2
Effect of temperature on seed germination of Bombax costatum Pellegr. & Vuill. (Malvales: Malvaceae) from Ondo and Oyo States, Nigeria 温度对黄颡鱼种子萌发的影响。& Vuill。(马尔代夫语:Malvaceae)来自尼日利亚翁多州和奥约州
Pub Date : 2019-08-31 DOI: 10.21472/bjbs.061311
M. Ojo
Effect of temperature on seed germination of Bombax costatum Pellegr & Vuill. (Malvales: Malvaceae), from four provenances in South Western Nigeria was investigated. A set of three replicates of 25 seeds per provenance were used for the experiment which was laid in a completely randomized block design. The seeds were exposed to fire temperature regimes for germination tests: 28 oC, 20 oC, 5 oC, 35 oC and 20 oC. Seed germination was recorded for 4 weeks. Data obtained were subjected to analysis of variance. Result showed that significant differences in seed germination under varying temperature regimes (P < 0.05). Interaction effect between temperature and provenances was not significant (P < 0.05). Seeds placed in ambient temperature had the highest mean number of germination (20.75) while the least mean value (0.17) was observed for cold room (-20 oC). B. costatum seeds should be exposed to ambient temperature so as to promote seed germination.
温度对黄颡鱼种子萌发的影响。(Malvales: Malvaceae),来自尼日利亚西南部的四个种源。试验采用完全随机区组设计,每个种源设置3个重复,每个重复25粒种子。将种子暴露在28℃、20℃、5℃、35℃和20℃的火温下进行发芽试验。记录种子萌发4周。所得数据进行方差分析。结果表明,不同温度下种子萌发率差异显著(P < 0.05)。温度与种源间互作效应不显著(P < 0.05)。常温下种子平均发芽率最高(20.75),低温(-20℃)萌发率最低(0.17)。海参种子应置于环境温度下,以促进种子萌发。
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引用次数: 0
Evaluation of bat-related knowledge, perceptions, and practices in an urban community: A strategy for Conservation Biology and health promotion 城市社区蝙蝠相关知识、认知和实践的评估:保护生物学和健康促进策略
Pub Date : 2019-08-31 DOI: 10.21472/bjbs.061303
Norlan Santos, E. Paz, I. Carneiro, C. Franke
The aim of this ethnozoological study was to identify variables related to knowledge, perceptions, and practices associated with the occurrence of bats in an urban area. We carried a retrospective observational study with residents of 31 cases and 178 control households. We defined the cases as households where accidents occurred from 2012 to 2015, according to official health records. The control households have no accident record in the same area. We conducted data collection through a semi-structured questionnaire and performed descriptive analyses and proportion (chi-square) to identify differences between the responses of cases and controls. We have identified important gaps in relation to basic knowledge, good practices, and the importance of bats to public health. These findings should be targeted for intervention and correction by environmental educators and health managers aimed at reducing the risk of accidents with bats and zoonosis, mainly rabies.
本民族动物学研究的目的是确定与城市地区蝙蝠发生相关的知识、观念和实践相关的变量。我们对31例病例的居民和178个对照家庭进行了回顾性观察研究。根据官方健康记录,我们将这些病例定义为2012年至2015年间发生事故的家庭。对照家庭在同一地区无事故记录。我们通过半结构化问卷收集数据,并进行描述性分析和比例(卡方),以确定病例和对照组之间的差异。我们已经确定了在基本知识、良好做法和蝙蝠对公共卫生的重要性方面的重要差距。环境教育工作者和卫生管理人员应该针对这些发现进行干预和纠正,以减少蝙蝠和人畜共患病(主要是狂犬病)事故的风险。
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引用次数: 3
Effect of moderate exercise on renal changes and oxidative stress in ovariectomized rats with type 1 diabetes mellitus 适度运动对1型糖尿病去卵巢大鼠肾脏变化及氧化应激的影响
Pub Date : 2019-08-31 DOI: 10.21472/bjbs.061302
M. Silveira, L. S. B. Amaral, Samira Itana de Souza, Halanna Rocha Ferraz, Jéssica Alves Dias, Edilene Cândido Rocha, F. A. Silva, A. M. Magalhães, T. D. J. Soares
This study evaluated the aerobic exercise effects of moderate and progressive intensity on renal function and structure, and oxidative stress in ovariectomized rats with type 1 diabetes mellitus induced by streptozotocin (STZ). Eighteen Wistar rats were divided into 3 groups: OSC - ovariectomized and sedentary control rats; OSD - ovariectomized and sedentary diabetic rats; and OTD - ovariectomized and trained diabetic rats. After induction of diabetes, the OTD group was submitted to eight weeks of exercise. Twenty-four hours after the last training session urine samples were collected. Blood samples and kidneys were collected after euthanasia for renal function analysis, histology, morphometry and oxidative stress. Our results have shown a reduction of the weight gain, increase of kidney weight and postprandial glycemia in diabetic rats. However, exercise decreased glycosuria and prevented the proteinuria in OTD group rats. Focal segmental glomerulosclerosis (FSGS), juxtamedullary glomerular tuft area, tubulointerstitial lesions (TIL), brush border loss and tubular cell debridement were reduced in OTD rats. In addition, exercise training decreased urinary and plasma concentrations of thiobarbituric acid reactive substance (TBARS). Our results demonstrate the beneficial effect of progressive aerobic exercise on proteinuria, glycosuria, and renal structure in ovariectomized diabetic rats, which may be mediated in part by reduction of oxidative stress.
本研究探讨了中等强度和进行性有氧运动对链脲佐菌素(STZ)诱导的1型糖尿病去卵巢大鼠肾脏功能、结构和氧化应激的影响。18只Wistar大鼠分为3组:OSC -卵巢切除组和久坐对照组;OSD -去卵巢和久坐的糖尿病大鼠;和OTD -卵巢切除和训练糖尿病大鼠。诱发糖尿病后,OTD组进行为期8周的运动。在最后一次训练后24小时采集尿样。安乐死后采集血液和肾脏进行肾功能分析、组织学、形态学和氧化应激分析。我们的研究结果显示,糖尿病大鼠体重增加,肾脏重量增加和餐后血糖下降。然而,运动可以减少OTD组大鼠的糖尿,并预防蛋白尿。OTD大鼠局灶节段性肾小球硬化(FSGS)、近髓肾小球簇面积、小管间质病变(TIL)、刷状边界丧失和小管细胞清创均减少。此外,运动训练降低尿和血浆中硫代巴比妥酸反应性物质(TBARS)的浓度。我们的研究结果表明渐进式有氧运动对去卵巢糖尿病大鼠的蛋白尿、糖尿和肾脏结构的有益影响,这可能部分是通过减少氧化应激介导的。
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引用次数: 2
期刊
Brazilian Journal of Biological Sciences
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