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Incidence of Measles Across the Population in Lviv Region in Ukraine 乌克兰利沃夫地区人口麻疹发病率
Pub Date : 2019-09-30 DOI: 10.33425/2639-9458.1065
N. Ivanchenko, Mariann Veres, N. Gonchar
In spite of vaccinal prevention, measles remains one of burning issues of public health service in Ukraine and Lviv region. According to statistics, increased measles cases in Ukraine is observing each 5-6 years. The predominant cause of incidence across the population is low preventive vaccination [3]. During 2017-2018-2019 in Lviv region there is the complicated epidemiological situation with measles incidence. In 2017 in Lviv region only 92 measles cases were recorded, but in 2018 11 474 cases (451.54 per 100 thousand people). Among the number of the diseased 8243 (72%) constitutes children. During 6 months in 2019, 6 024 people have diseased, including 4126 children, 1898 adults (218.62 per 100 thousand people). Among diseased children 73.7 % was not vaccinated according to the existing Calendar of Preventive Vaccination. 4 fatal cases have been recorded (2 in 2018 and 2 in 2019).
尽管进行了疫苗预防,麻疹仍然是乌克兰和利沃夫地区公共卫生服务的热点问题之一。据统计,乌克兰的麻疹病例每5-6年就会增加一次。人群发病率的主要原因是预防性疫苗接种率低[3]。2017-2018-2019年期间,利沃夫地区麻疹发病率的流行病学情况复杂。2017年,利沃夫地区仅记录了92例麻疹病例,但2018年为11474例(每10万人451.54例)。在患病人数中,8243人(72%)是儿童。在2019年的6个月里,有62024人患病,其中包括4126名儿童和1898名成年人(每10万人218.62人)。根据现有的预防性疫苗接种日历,患病儿童中73.7%没有接种疫苗。记录了4例死亡病例(2018年2例,2019年2例)。
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引用次数: 0
Organ Shortage- A Health Social Crisis. Causes, Outcomes and Proposals 器官短缺——健康社会危机。原因、结果和建议
Pub Date : 2019-09-30 DOI: 10.33425/2639-9458.1062
F. Cantarovich
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引用次数: 1
Evaluation of A Novel IgM Screening Enzyme-Linked Immunosorbent Assay for Sensitive Detection of Mayaro Virus-Infected Patients 一种新型IgM筛选酶联免疫吸附法对Mayaro病毒感染患者敏感检测的评价
Pub Date : 2019-06-30 DOI: 10.33425/2639-9458.1059
M. M., Freitas Henriques D, Abreu Lima J, C. L, Muigg N, K. O, Saschenbrecker S, Schlumberger W, S. K.
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引用次数: 0
Characterization of New Bacteriophages of Salmonella and Shigella For Polivalent Intestinal “Enco Bacterophage” Preparation 多价肠“Enco噬菌体”制备中沙门氏菌和志贺氏菌新型噬菌体的鉴定
Pub Date : 2019-06-30 DOI: 10.33425/2639-9458.1056
Gabisonia T, G. I, Topuria Nakaidze N, Loladze M, C. N, N. M, E. T, Kalandarishvili T, Tamarashvili N, Katamadze T
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引用次数: 0
A Simple Method to Immortalize Human Leukocytes and its Potential Applications 一种使人白细胞永生的简单方法及其潜在应用
Pub Date : 2019-06-30 DOI: 10.33425/2639-9458.1057
Elida Cleyse Gomes da Mata, Kanzaki L.I.B
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引用次数: 0
Prevalence and Antibiogram of Staphylococcus Aureus Isolated from Herd of Goat, Cow and Ram at Obinze, Imo State, Nigeria 尼日利亚伊莫州奥宾泽山羊、奶牛和公羊群分离的金黄色葡萄球菌的流行率和抗体谱
Pub Date : 2019-06-30 DOI: 10.33425/2639-9458.1058
Chijioke A. Nsofor
This study focused on the prevalence and antibiotic susceptibility pattern of Staphylococcus aureus isolated from livestock in Obinze, Owerri Imo State Nigeria. A total of 100 nasal swabs sample were collected, 86 samples were from goat, 9 samples were from cow and five samples were from ram. The samples were inoculated onto mannitol salt agar and the culture plates were incubated at 37 °C for 24 hours. The colonies showing yellow colour on mannitol salt agar were presumably identified as Staphylococcus spp; S. aureus was identified by biochemical tests. Antibiotics susceptibility pattern was determined by the disc diffusion method. The result of overall prevalence of the Staphylococcus aureus was 64%, with 62.79% isolates from goat sample, 66.67% isolates from cow sample and 80% isolates from ram samples. The frequency of resistance to antibiotics as observed in the animals were Sulphamethoxazole Trimethoprim 6 (12.77%), Nalidixic acid 32 (68.09%), Erythromycin 9 (19.15%), Amoxicillin/Clavulanic acid 16 (34.04%), Amoxicillin 17 (36.17%), Oxacillin 26 (55.32%), Gentamycin 9 (19.15%), Ciprofloxacin 6 (12.77%), Norfloxacin 20 (42.55%), Ampicilin19 (40.43%), Streptomycin 17 (36.17%). Sulphamethoxazole-Trimethoprim and Ciprofloxacin were the most sensitive antibiotics. The antimicrobial resistant pattern of Staphylococcus aureus isolate from the animals showed that 7 (14.89%) of the isolate were resistant to only one antibiotic and 2 (4.26%) isolates were resistant to 2 antibiotics and multidrug resistance was observed in 24 (51.07%) isolate of from goat, cow and ram giving a total of 33 (70.22%) resistant to antimicrobial agent respectively. The high prevalence of resistant strains of Staphylococcus aureus observed in this study may play a potential role of disseminating antibiotics resistant traits between animals and humans in the study area. Therefore, continued surveillance of this resistant strain of Staphylococcus aureus is very important for early prevention and control of community acquired infection. Keyword Staphylococcus aureus, Antibiotic resistance, Nigeria. Introduction Domestic livestock serve as a main source of food for man, as so many of their products such as milk and meat are essential raw materials in food production industries and in the preparation of many other consumables. The ability of Staphylococcus aureus to grow and produce staphylococcal enterotoxins (SEs) under a wide range of conditions is evident from the variety of foods implicated in the staphylococcal food poisoning (SFP) [1]. Similarly, there have been some reports indicating that domestic animals are the source of human infections, particularly humans in close contact with the animals either through the nature of their occupation or keeping the animals as pet. In this regard, the bacterial pathogen Staphylococcus aureus is one of the most important bacteria; particularly its methicillin resistant strain [2]. There is also evidence of the role of food animals in
本研究的重点是从尼日利亚奥韦里-伊莫州奥宾泽的牲畜中分离出的金黄色葡萄球菌的流行率和抗生素易感性模式。共采集了100份鼻拭子样本,86份来自山羊,9份来自奶牛,5份来自公羊。将样品接种在甘露醇盐琼脂上,并将培养板在37°C下孵育24小时。在甘露醇盐琼脂上显示黄色的菌落可能被鉴定为葡萄球菌属;金黄色葡萄球菌经生化鉴定。用纸片扩散法测定抗生素的药敏模式。金黄色葡萄球菌的总体流行率为64%,其中62.79%的分离株来自山羊样本,66.67%的分离株源自奶牛样本,80%的分离株取自公羊样本。在动物中观察到的抗生素耐药性频率为:磺胺甲恶唑-甲氧苄啶6(12.77%)、那利西酸32(68.09%)、红霉素9(19.15%)、阿莫西林/克拉维酸16(34.04%)、阿莫青霉素17(36.17%)、奥西林26(55.32%)、庆大霉素9(19.15%)、环丙沙星6(12.77%)、诺氟沙星20(42.55%)、氨苄19(40.43%),链霉素17(36.17%)、磺胺甲恶唑甲氧苄啶和环丙沙星是最敏感的抗生素。动物金黄色葡萄球菌分离株的耐药性模式显示,7株(14.89%)仅对一种抗生素耐药,2株(4.26%)对两种抗生素耐药。山羊、奶牛和公羊的24株(51.07%)分离株对多药耐药性分别为33株(70.22%)。本研究中观察到的金黄色葡萄球菌耐药性菌株的高流行率可能在研究区域的动物和人类之间传播抗生素耐药性特征方面发挥了潜在作用。因此,对这种耐药金黄色葡萄球菌菌株的持续监测对于早期预防和控制社区获得性感染非常重要。关键词金黄色葡萄球菌,抗生素耐药性,尼日利亚。引言家畜是人类的主要食物来源,因为它们的许多产品,如牛奶和肉,是食品生产行业和许多其他消耗品的制备中必不可少的原材料。金黄色葡萄球菌在各种条件下生长和产生葡萄球菌肠毒素(SE)的能力从与葡萄球菌食物中毒(SFP)有关的各种食物中可以明显看出[1]。同样,有一些报告表明,家畜是人类感染的来源,尤其是通过其职业性质或将动物作为宠物饲养而与动物密切接触的人类。在这方面,细菌病原体金黄色葡萄球菌是最重要的细菌之一;特别是其耐甲氧西林菌株[2]。在一些国家,也有证据表明食用动物在人类耐甲氧西林金黄色葡萄球菌(MRSA)中的作用,以及宠物可能是人类感染的来源。一些与动物密切合作的个体群体,如兽医,MRSA定植率很高[3,4]。随着肉类和畜牧业向更大规模的生产转移,抗生素生长药物的使用已经扩大。这些药物被添加到动物饲料中,以帮助它们长得更大以供屠宰,降低牲畜的脂肪并提高蛋白质含量。它们还可以预防大肠杆菌、沙门氏菌等细菌
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引用次数: 0
The Essential Role of Biofilms in Alzheimer’s Disease 生物膜在阿尔茨海默病中的重要作用
Pub Date : 2019-06-30 DOI: 10.33425/2639-9458.1060
H. Allen
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引用次数: 4
Dengue Serotypes in Bangladesh: Whole Genome Sequencing and Comparative Genomics Facilitates Pathogenesis and Epidemiology Studies and Informs Improved Disease Control 孟加拉国登革热血清型:全基因组测序和比较基因组学促进发病机制和流行病学研究,并为改善疾病控制提供信息
Pub Date : 2019-03-27 DOI: 10.33425/2639-9458.1054
Tahmina Tabassum, A. Taylor-Robinson
Dengue virus (DENV) is one of the most extensive arthropod-borne (arbo)viruses worldwide, especially prevalent in tropical and subtropical countries, and which is responsible for causing an array of clinical disease manifestations in humans. The increasing frequency of dengue outbreaks and the expanding range over which both the virus and its vector of transmission, female Aedes mosquitoes, are endemic, are pressing global public health concerns. The ongoing lack of either an efficacious vaccine or antiviral drug has contributed to the escalating problem. Inadequate knowledge of DENV genomic architecture and pathogenesis has proven to be a major drawback when designing effective prevention and treatment options. Mounting case numbers of dengue infection are reported each year in Bangladesh, yet no full-length genome sequence data are available for DENV isolates from this densely populated, developing South Asian nation. Sequencing and characterization of the whole genome of Bangladeshi DENV isolates of different virus serotypes is therefore an important priority in order to identify therapeutic target determinants against which to develop effective measures to combat the disease.
登革热病毒(DENV)是世界范围内最广泛的节肢动物传播病毒之一,尤其在热带和亚热带国家流行,并导致人类出现一系列临床疾病表现。登革热爆发的频率越来越高,病毒及其传播媒介雌性伊蚊的流行范围越来越广,这些都是全球公共卫生的紧迫问题。持续缺乏有效的疫苗或抗病毒药物导致了问题的升级。在设计有效的预防和治疗方案时,对登革病毒基因组结构和发病机制的了解不足已被证明是一个主要缺点。孟加拉国每年报告的登革热感染病例数都在增加,但目前还没有来自这个人口稠密、发展中的南亚国家的登革病毒分离株的全长基因组序列数据。因此,对不同病毒血清型的孟加拉国登革病毒分离株的全基因组进行测序和鉴定是确定治疗目标决定因素的重要优先事项,以制定有效措施来对抗该疾病。
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引用次数: 2
Prevalence of Methicillin Resistance Staphylococcus Aureus (MRSA) Infection and Incidence Rate of Diabetic MRSA Patients in Northern Region of KSA KSA北部地区耐甲氧西林金黄色葡萄球菌(MRSA)感染率和糖尿病MRSA患者发病率
Pub Date : 2019-03-27 DOI: 10.33425/2639-9458.1052
Fai Alassi Alanzi, Hanan O. Al Shammari, Samreen Soomro
Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium that causes infection in different parts of the body. It's tougher to treat than most strains of staphylococcus aureus. Because it's resistant to some commonly used antibiotics. Although enough data is available about MRSA prevalence worldwide but here is lack of research and accurate statistics that covers the north regions of Saudi Arabia. The aim of current research is to analyze the MRSA positive cases in Northern region of Saudi Arabia and to identify the diabetic patients with MRSA infection. Isolates of MRSA, recovered from various clinical samples from two different cities Rafha and Quriyat in the north region of Saudi Arabia. Samples were included in this prospective, cross-sectional study from Jan 2013 to June 2017. Agar diffusion method was employed according to the protocols of Clinical Laboratory Standards Institute. Out of total 800 S. aureus strains, the frequency rate of MRSA was 42.5% (n = 340). MRSA infection was found higher among the age group 20-40 years i.e. 33% (n=264), followed by 28% (n=224) in 60-80 years. Frequency of MRSA percentage in male and female was and 53% and 47%, respectively. MRSA was more frequently observed in eye sample 79% (n=632). MRSA showed resistance to antibiotics as cefuroxime (90.16%), streptomycin (59.3%), amikacin (95.8%), azithromycin (63.8%), ampicillin (95.8%), amoxicillin/clavulanic acid (76.4%), Penicillin G (96%), oxacillin (66%), gentamicin (68%), cefoxitine (94%) MRSA infection among diabetic patients showed significant prevalence that was found to be 30% and its alarming to stop the spread at this point. This study provided sufficient knowledge toward spread of multi drug resistant S. aureus against a number of antibiotics that were used to be sensitive before These results giving us a picture of real threat for spreading a life-threatening infection in the region if not taken serious action particularly the diabetic patients will be on challenge.
耐甲氧西林金黄色葡萄球菌(MRSA)是一种在身体不同部位引起感染的细菌。它比大多数金黄色葡萄球菌更难治疗。因为它对一些常用的抗生素有耐药性。尽管有足够的关于MRSA在全球范围内流行的数据,但缺乏涵盖沙特阿拉伯北部地区的研究和准确统计数据。本研究的目的是分析沙特阿拉伯北部地区MRSA阳性病例,并确定感染MRSA的糖尿病患者。耐甲氧西林金黄色葡萄球菌分离株,从沙特阿拉伯北部两个不同城市拉菲和库里亚特的各种临床样本中回收。样本包括在2013年1月至2017年6月的前瞻性横断面研究中。琼脂扩散法是根据临床实验室标准研究所的方案使用的。在总共800株金黄色葡萄球菌中,MRSA的发生率为42.5%(n=340)。MRSA感染在20-40岁年龄组中较高,即33%(n=264),其次是60-80岁年龄组的28%(n=224)。耐甲氧西林金黄色葡萄球菌在男性和女性中的发生率分别为53%和47%。在79%(n=632)的眼睛样本中更频繁地观察到MRSA。耐甲氧西林金黄色葡萄球菌对抗生素表现出耐药性,如头孢呋辛(90.16%)、链霉素(59.3%)、阿米卡星(95.8%)、阿奇霉素(63.8%)、氨苄青霉素(95.8%。这项研究为耐多药金黄色葡萄球菌对多种抗生素的传播提供了足够的知识,这些抗生素在以前是敏感的。这些结果让我们了解了如果不采取认真行动,特别是糖尿病患者将面临挑战,在该地区传播危及生命的感染的真正威胁。
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引用次数: 1
Enteral Anti-Tuberculosis Drugs Regimen Contributes to Mortality in Critical Patients with Smear Positive Pulmonary Tuberculosis 肠道抗结核药物方案对涂阳肺结核危重患者死亡率的影响
Pub Date : 2019-03-27 DOI: 10.33425/2639-9458.1051
Sueyanyongsiri P, Sueyanyongsiri S, S. N.
In severe pulmonary tuberculosis that requiring ventilation and critical care should have uncertain enteral absorption. This study aimed to evaluate the impact of enteral anti-tuberculosis regimen used on the survival of patients with smear positive pulmonary tuberculosis requiring mechanical ventilation. Patients aged >15 years with critical smear positive pulmonary tuberculosis were divided into enteral and parenteral antituberculosis drugs regimen groups based on the type of anti-tuberculosis used. Those patients who died or discharged within 24 hours of hospitalization were excluded. The primary endpoint was 40-day survival. In 5844 patients of tuberculosis from 2013 to 2018 have 675 patients identified that smear positive pulmonary tuberculosis, 657 were in enteral drugs group and 18 were in the parenteral fluoroquinolones group, 140 patients die and 535 patients survived. The two groups had statistically significant difference in acute respiratory failure and shock. There had no statistically significant difference between two groups in mortality by univariable risk ratio regression analysis. In generally parenteral regimens are increase mortality rate, but after adjusted all variable factors by multivariable risk ratio regression analysis, there had statistically significant difference between two groups in mortality (risk ratio=1.80; 95%confidence interval=1.25 to 2.58; P=0.001). The medial survival was 8 and 34 days in enteral and parenteral groups, significant difference in log rank test (P<0.002). Enteral anti-tuberculosis regimen may contribute to survival of smear positive pulmonary tuberculosis requiring mechanical ventilator.
在需要通气和重症监护的严重肺结核中,肠内吸收应该是不确定的。本研究旨在评估肠内抗结核方案对需要机械通气的涂阳肺结核患者生存的影响。年龄>15岁的危重涂阳肺结核患者根据使用的抗结核药物类型分为肠内和肠外抗结核药物方案组。那些在住院24小时内死亡或出院的患者被排除在外。主要终点为40天生存期。在2013年至2018年的5844名结核病患者中,675名患者被确定为涂阳肺结核,657名患者在肠内药物组,18名患者在胃肠外氟喹诺酮药物组,140名患者死亡,535名患者存活。两组在急性呼吸衰竭和休克方面有统计学意义的差异。通过单变量风险比回归分析,两组之间的死亡率没有统计学上的显著差异。在一般情况下,肠外方案的死亡率增加,但通过多变量风险比回归分析调整所有可变因素后,两组的死亡率有统计学显著差异(风险比=1.80;95%置信区间=1.25-2.58;P=0.001),log秩检验有显著性差异(P<0.002)。肠内抗结核方案可能有助于需要机械通气的涂阳肺结核患者的生存。
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引用次数: 0
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Microbiology & infectious diseases (Wilmington, Del.)
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