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Growth and feeding habits in reared Japanese centropomid fish Lates japonicus 日本拟心鱼的生长和摄食习性
Pub Date : 1995-01-01 DOI: 10.2331/SUISAN.61.684
K. Tashiro, Y. Iwatsuki
Fifty-eight young Japanese centropomid fish Lates japonicus were captured from the Hitotsuse River estuary, Miyazaki Prefecture, southeastern Kyushu, Japan, through April to September, 1989, and their sizes of total length (TL) (mean TL 222mm) and body weight (BW) (mean 147g) were recorded. They were reared in a concrete tank (20 ton) until the end of November, 1992, growing to a mean TL of 500mm and mean BW of 1, 825g. The diet of the fish was comprised mainly of raw sardines, shrimp, jack and mackerel. However, negative Feeding efficiency rates and Conversion fac-tors were apparent over the two winter seasons. Feeding activity, based on the mean quantity of food taken per individual, was closely related to water temperature and salinity (S) changes, especially being highest over about 25°C (S 28-30) and reduced to zero below about 16°C (S 34). The species seemed to be strongly resistant to disease, skin erosion and transportation stress, because all individ-uals used for the experiment survived.
1989年4 ~ 9月,在日本九州东南部宫崎县一濑河河口捕获日本形心鱼幼鱼58尾,记录其体长(TL)大小(平均TL 222mm)和体重(BW)(平均147g)。1992年11月底,它们被饲养在一个混凝土罐中(20吨),平均体长为500毫米,平均体重为1825克。这些鱼的食物主要包括生沙丁鱼、虾、杰克鱼和鲭鱼。然而,在两个冬季期间,采食效率和转化系数均呈现明显的负变化。基于个体平均摄取量的摄食活动与水温和盐度(S)变化密切相关,特别是在25°C (S 28-30)以上最高,在16°C (S 34)以下降为零。这个物种似乎对疾病、皮肤侵蚀和运输压力有很强的抵抗力,因为所有用于实验的个体都存活了下来。
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引用次数: 8
Effect of water activity and storage temparature on the quality and microflora of smoked salmon 水活度和贮藏温度对烟熏鲑鱼品质和菌群的影响
Pub Date : 1994-01-01 DOI: 10.2331/SUISAN.60.569
Tsukasa Shimasaki, K. Miake, Y. Tsukamasa, M. Sugiyama, Y. Minegishi, H. Shinano
Two types of smoked salmon in the Aw range above 0.93 and less than 0.96 (L-Aw type) and above 0.96 (H-Aw type) were prepared, sliced, vacuum-packed and then stored for 40, 30, and 5 days at 5, 10, and 20°C, respectively. Changes in sensory evaluation, and microbiological and chemical characteristics were investigated throughout the storage period. The overall sensory score and textural evaluation of the H-Aw type decreased faster than the L-Aw type at each storage temperature. Viable cell counts and VB-N values of the H-Aw type were larger than the L-Aw type at each temperature during the storage period. As for microflora in the H-Aw type, Enterobacteriaceae was significant in the middle period at 10°C, and was very common at 20°C after 2 days of storage. Lactobacillus finally dominated under the temperature conditions examined. In the L-Aw type, Streptococcus and Lactobacillus were predominant on the last day of storage at 20°C, and 10 and 5°C storage, respectively. These findings suggest that the storage temperature and Aw of smoked salmon are closely correlated to shelf-life, because a combination of storage temperature and Aw affected the viable cell counts and formation of microflora during the storage period and induced the changes of sensory evaluation and VB-N values.
将Aw值在0.93以上、0.96以下(L-Aw型)和0.96以上(H-Aw型)的两种烟熏三文鱼分别制备、切片、真空包装,在5℃、10℃和20℃条件下分别保存40、30和5 d。在整个贮藏期间,研究了感官评价、微生物学和化学特性的变化。在不同的贮藏温度下,H-Aw型的整体感官评分和质地评价下降速度均快于L-Aw型。贮藏期间各温度下H-Aw型的活细胞数和VB-N值均大于L-Aw型。H-Aw型菌群中,10℃中期以Enterobacteriaceae为主,20℃保存2 d后最为常见。乳杆菌在温度条件下最终占主导地位。在L-Aw型中,20℃、10℃和5℃贮藏的最后一天,链球菌和乳酸杆菌分别占主导地位。综上所示,烟熏三文鱼的贮藏温度和湿度与货架期密切相关,因为贮藏温度和湿度的组合影响了烟熏三文鱼在贮藏期间的活细胞数量和微生物区系的形成,并引起感官评价和VB-N值的变化。
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引用次数: 16
Factors inhibiting growth of the rotifer Brachionus plicatilis in high density cultivation by feeding condensed Chlorella 高密度饲养浓缩小球藻对轮虫繁殖的抑制因素
Pub Date : 1994-01-01 DOI: 10.2331/SUISAN.60.207
K. Yoshimura, C. Kitajima, Y. Miyamoto, G. Kishimoto
In order to investigate the factors inhibiting the growth of rotifer (S-type) in high density cultivation, a series of experiments were conducted, cultivating rotifer in 30l and 1kl vessels by feeding condensed Chlorella. Although there was no difference in the maximum population of rotifer in the culture experiments at different temperatures of 24, 28, and 32°C, the growth rate increased as the culture temperature increased. In the cultivation system supplied with air, the maximum rotifer population did not exceed 4, 000-5, 000 individuals/ml because oxygen levels were restricted due to the accumulation of undissociated ammonia. Feeding oxygen gas to the rotifer culture system enabled high density cultivation with a population of 20, 000 individuals/ml. In such an oxygen-rich culture system, the concentration of undissociated ammonia is considered to be the main factor inhibiting the growth of rotifer.
为了研究高密度培养条件下抑制s型轮虫生长的因素,在30l和1kl的容器中饲喂浓缩小球藻培养轮虫。24℃、28℃和32℃培养实验中轮虫的最大种群数量虽然没有差异,但随着培养温度的升高,生长速度增加。在提供空气的栽培系统中,由于未解离氨的积累限制了氧气水平,最大轮虫种群不超过4000 - 5000只/ml。向轮虫培养系统提供氧气,实现了高密度培养,种群数量为20,000只/ml。在这种富氧培养体系中,未解离氨的浓度被认为是抑制轮虫生长的主要因素。
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引用次数: 21
Lipid class and fatty acid composition of liver of firefly squid Watasenia scintillans 萤火鱿鱼肝脏脂类及脂肪酸组成
Pub Date : 1994-01-01 DOI: 10.2331/SUISAN.60.247
K. Kawasaki, T. Ooizumi, Seishiro Hayashi, Kenji Hayashi
The lipid content and composition of the liver of firefly squid Watasenia scintillans from the Sea of Japan were investigated. The amount of liver lipids of firefly squid increased from March to April and then decreased in May or June. From March to July the liver of firefly squid from Toyama Bay contained a larger amount of lipids than that from Wakasa Bay. The liver lipids of firefly squid were characterized by high levels of triglycerides (60.9-72.7%) with small amounts of other components. The lipid class composition was more or less constant in spite of the differences in the fishing ground and season. The component fatty acids of liver lipids consisted of saturates (22.4-26.4%), monoenes (37.5-45.6%), and polyenes (29.9-37.8%). The major fatty acids were palmitic (13.6-16.3%), palmitoleic (6.5-9.2%), oleic (16.3-21.5%), eicosenoic (5.3-9.3%), eicosapentaenoic (13.0-20.0%), and docosahexaenoic (7.6-12.3%) acids. Firefly squid from Toyama Bay contained higher percentages of eicosapentaenoic acid than that from Wakasa Bay. The seasonal change in the eicosapentaenoic acid level was coincident with that of the lipid contents in the liver.
研究了日本海鱿鱼(Watasenia scintillans)肝脏的脂质含量和组成。3 ~ 4月鱿鱼肝脂含量呈上升趋势,5、6月呈下降趋势。3 ~ 7月富山湾鱿鱼肝脏脂质含量高于若浅湾鱿鱼肝脏脂质含量。鱿鱼肝脂的特点是甘油三酯含量高(60.9 ~ 72.7%),其他成分含量少。尽管渔场和季节不同,但脂类组成基本不变。肝脏脂质脂肪酸组成主要为饱和脂肪酸(22.4-26.4%)、单烯脂肪酸(37.5-45.6%)和多烯脂肪酸(29.9-37.8%)。主要脂肪酸为棕榈酸(13.6 ~ 16.3%)、棕榈油酸(6.5 ~ 9.2%)、油酸(16.3 ~ 21.5%)、二十烯酸(5.3 ~ 9.3%)、二十碳五烯酸(13.0 ~ 20.0%)和二十二碳六烯酸(7.6 ~ 12.3%)。富山湾鱿鱼中二十碳五烯酸的含量高于若浅湾鱿鱼。二十碳五烯酸水平的季节变化与肝脏脂质含量的季节变化一致。
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引用次数: 8
Identification of proteolytic activities of gel-degrading factors in three lizardfish species [Saurida undosquamis, S. wanieso, S. elongata] 三种蜥蜴鱼凝胶降解因子蛋白水解活性的鉴定[Saurida undosquamis, S. wanieso, S. elongata]
Pub Date : 1993-01-01 DOI: 10.2331/SUISAN.59.1039
Pantip Suwansakornkul, Y. Itoh, S. Hara, Atsushi Obatake
The inhibitory effects of several selected proteinase inhibitors, soybean trypsin inhibitor, leupeptin, antipain, E-64, NEM and EDTA, on myosin heavy chain (MHC) degradation of unwashed and washed meat gels from three lizardfish species, maeso or true lizardfish Saurida undosquamis, wanieso Saurida wanieso, tokageeso or shortfin lizardfish Saurida elongata, were investigated in order to identify the proteolytic activities of gel-degrading factors (GDFs) existing in mat. It was apparent that the proteolytic activities of GDFs responsible for MHC degradation of meat gels from all species at 40°C resulted from two myofibril-associated (Mf) type proteinases, mainly of serine proteinase and slightly of cysteine proteinase. In addition, as indicated by the amino acid patterns obtained from TCA-soluble fraction of meat gels with the presence of various inhibitors, these proposed proteinases were not distinct in these three species. With regard to the MHC degradation at 60°C, the proteolytic activity present in GDFs of maeso, proposed as myofibril-associated gel degrading factors (Mf-GDFs), belonged to cysteine proteinase while that of wanieso and tokageeso were sarcoplasmic-type serine proteinases. The Mf-GDF corresponding to the MHC degradation at 60°C, which occasionally occurs in wanieso meat gel, showed the proteolytic activities of serine proteinase.
几种选定的蛋白酶抑制剂(大豆胰蛋白酶抑制剂、胰肽、抗痛剂、E-64、NEM和EDTA)对三种蜥蜴鱼(maeso或true lizardfish Saurida undosquamis、wanieso Saurida wanieso、tokageeso或shortfinlizardfish Saurida elongata)未洗涤和洗涤肉凝胶中肌球蛋白重链(MHC)降解的抑制作用。结果表明,在40°C条件下,所有种类肉类凝胶中降解MHC的凝胶降解因子(GDFs)的蛋白水解活性是由两种肌原纤维相关(Mf)型蛋白酶引起的,主要是丝氨酸蛋白酶,少量是半胱氨酸蛋白酶。此外,从含有各种抑制剂的肉凝胶的tca可溶性部分中获得的氨基酸模式表明,这些拟议的蛋白酶在这三个物种中并没有明显的差异。关于60℃下MHC的降解,maeso的GDFs中存在的蛋白水解活性被认为是肌原纤维相关凝胶降解因子(Mf-GDFs),属于半胱氨酸蛋白酶,而wanieso和tokageeso的蛋白水解活性为肌浆型丝氨酸蛋白酶。偶有发生在wanieso肉凝胶中的MHC 60℃降解对应的Mf-GDF显示出丝氨酸蛋白酶的蛋白水解活性。
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引用次数: 19
Attraction activities of fruit extracts for the oriental weatherfish Misgurnus anguillicaudatus 果实提取物对东方风信鱼的引诱作用
Pub Date : 1993-01-01 DOI: 10.2331/SUISAN.59.1757
K. Harada, T. Miysaki
The attaction activities of fruit-flesh or fruit-rind water extracts were statistically estimated on the basis of an exploratory behavior of the oriental weatherfish Misgurnus anguillicaudatus. Fruits tested were 36 forms of 25 species: 22 of 13 in Rosaceae, 6 of 6 in Rutaceae, 3 of 1 in Vitaceae, and 1 each in five other famillies (Actinidiaceae, Cucurbitaceae, Ebenaceae, Moraceae, and Myricaceae). Of fruit-flesh, the attraction activity in Rosaceae was high in eight forms: one apricot (adventitious seedling form), three cherries (Napoleon biggreau, hybrid and Prunus cerasus forms), one European plum (Soldum form), one nectarine, one peach (adventitious seedling form), and one strawberry. The attraction in Rutaceae was high only in unshiu orange, but was low in two orange forms. The attraction in Vitaceae was found to be low in only one grape (Delaware form). The attraction in the other five families was low in kiwi fruit (Actinidiaceae), persimmon (Ebenaceae), and myrica (Myricaceae). Of the fruit extracts tested, strawberry was found to be highest in attraction, though its attraction activity depended on the conentration. Of fruit-rind, the attraction in Rutaceae was low only in unshiu orange. The relationship between the attraction effect and the characteristics of fruits is also discussed.
以东方风带鱼Misgurnus anguillicaudatus的诱虫行为为基础,统计估计了果肉水提取物和果皮水提取物的诱虫活性。试验果实为25种36种,蔷薇科13种中的22种,芦花科6种中的6种,Vitaceae 1种中的3种,其他5科(猕猴桃科、葫芦科、Ebenaceae、桑葚科和杨梅科)各1种。在果实果肉中,蔷薇科杏(不定苗型)1个、樱桃(拿破仑、杂种和樱李)3个、欧洲李(小李型)1个、油桃1个、桃(不定苗型)1个、草莓1个吸引活性较高。芦花科植物中,只有云秀橙的吸力高,两种橙的吸力都较低。在Vitaceae中,发现只有一种葡萄(Delaware)的吸引力较低。猕猴桃科(猕猴桃科)、柿子科(柿子科)和杨梅科(杨梅科)的吸力较低。在测试的水果提取物中,草莓被发现具有最高的吸引力,尽管它的吸引力活性取决于浓度。果皮对芸香科植物的吸引力较低,仅对云秀柑的吸引力较低。并讨论了引种效应与果实特性的关系。
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引用次数: 4
Migrating behaviour of fish schools in set-net fishing grounds 设网渔场内鱼群的迁徙行为
Pub Date : 1993-01-01 DOI: 10.2331/SUISAN.59.473
Mun-Kwan Kim, T. Arimoto, Y. Matsushita, Y. Inoue
The migrating behaviour of fish schools such as jack mackerel Trachurus japonicus, spotted mackerel Scomber australasicus, and Japanese anchovy Engraulis japonicus was investigated in set-net fishing grounds by means of sonar image records. The spotted mackerel and Japanese anchovy schools moved at an average speed of 18-19cm/s, while the jack mackerel schools moved at 29cm/s. Schools of all species changed their direction of movement at an average veering angle of 19 ?? 36°. However, the veering angle of Japanese anchovy schools showed a tendency to be larger than that of the other two species. The average duration of straight movement of the spotted mackerel and Japanese anchovy schools was 2 ?? 2.4min, while that of the jack mackerel schools was 2.9min. This confirms a frequent veering of fish schools in the set-net fishing grounds. The jack mackerel and Japanese anchovy schools tend to move along the isobathic route with a depth change ratio of 0.6 ?? 0.8m/min between the first and final image. In case of the spotted mackerel schools the larger depth change was observed up to 6m/min.
采用声纳图像记录的方法,研究了在渔场设网渔场的长尾鲭鱼、斑点鲭鱼、日本凤尾鱼等鱼群的洄游行为。斑点鲭鱼和日本凤尾鱼鱼群的平均移动速度为18-19厘米/秒,而长尾鲭鱼鱼群的移动速度为29厘米/秒。所有物种的鱼群改变它们的运动方向,平均转向角度为19 ??36°。然而,日本凤尾鱼鱼群的转向角度呈现出比其他两个物种更大的趋势。斑点鲭鱼和日本凤尾鱼鱼群的平均直线运动时间为2 ??青花鱼组为2.4min,青花鱼组为2.9min。这证实了固定渔网渔场的鱼群经常转向。青花鱼和日本凤尾鱼鱼群倾向于沿等深路线移动,深度变化比为0.6 ??第一张图像和最终图像之间的差距为0.8m/min。斑点鲭鱼鱼群的深度变化较大,可达6米/分钟。
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引用次数: 4
Spawning, eggs, larvae and juveniles of the purplish amberjak Seriola dumerili 紫色琥珀的产卵、卵、幼虫和幼鱼
Pub Date : 1993-01-01 DOI: 10.2331/SUISAN.59.1479
K. Tachihara, Ryousei Ebisu, Yasuo Tukashima
The spawning, embryonic development, and morphological changes of the purplish amberjack Seriola dumerili were observed using specimens reared at the Aquaculture Research Laboratory of Nagasaki Prefectural Institute of Fisheries. Spawning was induced by intra-mascular injection of pituitary homogenate of the chum salmon Oncorhynchus keta and the genital hormone. Spawning occurred 36 hours after hormone treatment and 1, 400, 000 and 7, 000, 000 eggs were obtained in 1990 and 1991, respectively. The eggs were pelagic, spherical, and 1.05-1.20mm in diameter with a single oil globule of 0.22-0.28mm. The larvae hatched out 37 hours after spawning (W. T. 24.6°C). The newly hatched larvae were 2.7-3.1mm in length with 26 myotomes and a single oil globule. The yolk was mostly absorbed on the 4th day after hatching when the larvae attained 3.9mm in length and began to feed on rotifers. In the postlarval stage (6.3mm B. L.) the rudiments of ventral, dorsal, and anal fins began to develop and the vertebra was well ossified. In the juvenile stage (11.2mm B. L.) the membranous fins disappeared and fish gathered around drifting algae. Later in the juvenile stage (35.0mm B. L.) six or seven lateral bands appeared in the trunk as well as a band from the eye to the anterior part of the dorsal fin base. At a body length of 100mm, they reached the stage of young fish, while at a size of over 170mm in body length a yellow vertical stripe appeared from behind the operculum to the caudal peduncle. The smallest size of sexual maturation was thought to be about 600mm. Notochord or standard length at each growth stage was as follows: 2.7-3.9mm (yolk sac stage), 3.9-4.7mm (preflexion stage), 4.8-8.5mm (postflexion stage), 10-100mm (juvenile stage), 100-600mm (young stage), and over 600mm (adult stage).
利用长崎县水产研究所水产养殖研究室饲养的标本,观察了紫琥珀的产卵、胚胎发育和形态变化。用大马哈鱼垂体匀浆和生殖激素肌内注射诱导产卵。在激素处理后36小时产卵,1990年和1991年分别获得140万和700万枚卵。卵为远洋球形,直径1.05-1.20mm,单个油球0.22-0.28mm。产卵37 h后孵化,温度24.6℃。新孵化的幼虫体长2.7 ~ 3.1mm,肌切体26个,油球1个。孵化后第4天,幼虫体长达到3.9mm,开始以轮虫为食,卵黄基本吸收。在幼虫后期(6.3mm b.l.),腹鳍、背鳍和肛鳍的雏形开始发育,椎体已经很好地骨化了。在幼鱼期(11.2mm b.l),膜鳍消失,鱼类聚集在漂流藻周围。幼鱼期(胸径35.0mm),躯干出现六七条侧带,从眼部到背鳍基部前部出现一条侧带。体长100毫米时为幼鱼期,体长超过170毫米时,体盖后至尾柄处出现黄色垂直条纹。性成熟的最小尺寸被认为约为600毫米。各生长阶段脊索或标准长度分别为:卵囊期2.7 ~ 3.9mm、前屈期3.9 ~ 4.7mm、后屈期4.8 ~ 8.5mm、幼期10 ~ 100mm、幼期100 ~ 600mm、成虫期600mm以上。
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引用次数: 42
Alternative protein sources for fish meal in diets of young yellowtail 幼黄尾鱼饲料中鱼粉蛋白质的替代来源
Pub Date : 1993-01-01 DOI: 10.2331/SUISAN.59.137
S. Shimeno, T. Masumoto, T. Hujita, T. Mima, S. Ueno
Meat meal, meat-and-bone meal, corn gluten meal, and rapeseed meal were evaluated individually as partial replacement for fish meal in single moist pellet diets for young yellowtail Seriola quinqueradiata. Brown fish meal in the diet was replaced isonitrogenously and isocaloricaly with 10, 20, and 30% of each source. Better growth and feed efficiency were found in fish fed on diets with replacement of 10% of all the protein sources used, and the performance further increased as the replacement with meat meal was increased up to 30%. On the other hand, the performance tended to decrease as the replacement of other sources were increased to levels of more than 10%, and the worst performance together with diminished blood and serum characteristics were found in fish fed on the diet with 30% rapeseed meal. These results show that fish meal can be replaced with several cheaper proteins such as meat meal and corn gluten meal within limited amounts, and that the adequate addition of these protein sources to fish meal-based diets improves not only the dietary cost but also their performance.
分别评价了肉粉、肉骨粉、玉米蛋白粉和油菜籽粉在单湿颗粒饲料中部分替代鱼粉的效果。用10%、20%和30%的褐鱼粉等氮、等热量替代饲料中的褐鱼粉。在替代10%蛋白质源的饲粮中,鱼的生长和饲料效率较好,当替代30%的肉粉时,性能进一步提高。另一方面,当其他来源替代量增加到10%以上时,生产性能呈下降趋势,在添加30%油菜籽粕的饲料中,生产性能最差,血液和血清特性下降。上述结果表明,在一定数量的范围内,可以用几种更便宜的蛋白质如肉粉和玉米蛋白粉代替鱼粉,在鱼粉为基础的饲料中适当添加这些蛋白质来源不仅可以提高饲料成本,而且可以提高饲料的生产性能。
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引用次数: 89
Age and growth of the sunray surf clam Mactra chinensis in Tomakomai, southwest Hokkaido [Japan] 日本北海道西南部Tomakomai海蛤的年龄和生长
Pub Date : 1993-01-01 DOI: 10.2331/SUISAN.59.469
I. Sakurai
The age and growth of the sunray surf clam Mactra chinensis in Tomakomai were studied by measuring shell rings. Monthly measurement of shell increment from the last ring indicated ring formation to occur annually from November to January when seawater temperature dropped below about 10°C, and thus to be regardable as an age indicator. Age at the first ring formation was estimated at 0 by observing the shell rings of newly settled clams. Age was determined by counting the number of rings, and the relationship between age and shell length was expressed using von Bertalanffy's growth equation: Lt=78.31 [1-exp{-0.67(t-0.34)}], Lt being shell length (mm) at age t (years).
采用测量壳环的方法,研究了Tomakomai海蛤(Mactra chinensis)的年龄和生长。每月从最后一个环开始测量贝壳增量,表明每年11月至次年1月海水温度低于10℃左右时形成环,因此可作为年龄指标。通过观察新定居蛤的壳环,估计第一次环形成的年龄为0岁。年龄通过计算环数来确定,年龄与壳长之间的关系用von Bertalanffy生长方程表示:Lt=78.31 [1-exp{-0.67(t-0.34)}], Lt为年龄t(年)时的壳长(mm)。
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引用次数: 10
期刊
Bulletin of the Japanese Society of Scientific Fisheries
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