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Bulletin of the Japanese Society of Scientific Fisheries最新文献

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Distribution of juxtaglomerular cells in the kidney of the red batfish [Halieutaea stellata] 红蝠鱼肾脏肾小球旁细胞的分布
Pub Date : 1993-01-01 DOI: 10.2331/SUISAN.59.35
M. Oguri
Histologic examination was made on the distribution pattern of juxtaglomerular (JG) cells in the aglomerular kidney of the red batfish Halieutaea stellata. In this examination, five specimens (71 to 183g in body weight) were used and serial histologic sections were prepared from left and right kidneys (0.26to 0.89g in combined weight) of these specimens. From detailed histologic observation performed on the whole sections, it was ascertained that JG cells were localized as clusters in several regions within the kidney and the number of cell clusters was 3 to 10 and 4 to 10 in the left and right kidney tissues, respectively.
对星状红蝠鱼肾小球旁细胞(JG)的分布规律进行组织学检查。本实验共取5个标本(体重71 ~ 183g),分别取左肾和右肾(总重量0.26 ~ 0.89g)作连续组织学切片。通过对整个切片进行详细的组织学观察,确定JG细胞在肾脏内的多个区域呈簇状分布,左肾和右肾组织的细胞簇数分别为3 ~ 10个和4 ~ 10个。
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引用次数: 0
Regeneration from segments of sporelings in Myagropsis myagroides and Sargassum horneri Myagropsis myagroides和马尾藻sposporeles片段的再生
Pub Date : 1993-01-01 DOI: 10.2331/SUISAN.59.789
N. Nanba
Regeneration from segments of sporelings in Myagropsis myagroides and Sargassum horneri was studied in laboratory culture. They were cut into a series of four segments: first leaf, upper portion of stem with basal part of first leaf and second leaf, lower portion of stem with basal part of rhizoids, and rhizoids. These segments were cultured for 2 weeks at 20°C under three different light intensities with 12L/12D. In M. myagroides cultured at 2000-2800lx, rhizoids regenerated from segments of the first leaf and the upper portion of the stem. More-over, both rhizoids and adventitious branches regenerated from segments of the lower portion of the stem. In S. horneri cultured at 2000-2800lx, rhizoids regenerated from segments of the upper portion of the stem, while adventitious branches regenerated from segments of rhizoids. Furthermore, both rhizoids and adventitious branches regenerated from segments of the first leaf and the lower portion of the stem. These results indicate that segments of the upper and lower portion of the stem of M. myagroides and a series of four segments of S. horneri have totipotency. Regeneration of segments was inhibited in both species cultured in total darkness and at 200-3001x.
在实验室培养中研究了Myagropsis myagroides和马尾藻(Sargassum horneri)孢子的再生。它们被切成四段:第一叶、茎上部与第一叶和第二叶基部、茎下部与根状花序基部、根状花序基部。这些片段在20°C、12L/12D三种不同光强条件下培养2周。在2000-2800lx培养的myagroides中,从第一片叶子的片段和茎的上部再生出块根。此外,根状分枝和不定分枝都是从茎下部的节段再生的。在2000-2800lx培养的金针菇中,茎上部的部分再生为块根,而不定枝从块根的部分再生为块根。此外,块根和不定枝都是由第一片叶和茎下部的部分再生的。这些结果表明,密氏菌茎的上、下两个部分和角孢菌的一系列四个部分具有全能性。在全暗和200-3001x培养条件下,两种植物的节段再生均受到抑制。
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引用次数: 4
Quality evaluation of seasoning extracts on the basis of their beta-buffer capacity and amino acid composition 基于-缓冲能力和氨基酸组成的调味提取物质量评价
Pub Date : 1993-01-01 DOI: 10.2331/SUISAN.59.177
H. Ren, Tetsuhito Hayashi, H. Endo, E. Watanabe
Several seasoning extracts prepared from yeast and animal materials were evaluated on the basis of their β-buffer capacity and free amino acid composition instead of conventional quality indices, i.e. total nitrogen, salt, and moisture content. There was a proportional relationship between sample concentration and its β-buffer capacity. Comparison of β-buffer capacities among commercially available seasoning extracts, their deproteinized samples, and reconstructed amino acid solutions on the basis of their analytical data led us the following conclustions. Area size and maximum pH of each peak shown in a β-buffer curve suggested the degree of acid hydrolysis, major amino acid, and extent of fullness and extention in taste given by several oligopeptides and water soluble proteins. These findings suggest that β-buffer capacity reflects the chemical composition of electrolytes and the taste quality of the extract.
本文以酵母和动物原料为原料制备的几种调味料的β-缓冲能力和游离氨基酸组成代替传统的质量指标,即总氮、盐和水分含量,对其进行了评价。样品浓度与其β-缓冲容量成正比关系。在分析数据的基础上,对市售调味品提取物、脱蛋白样品和重组氨基酸溶液的β-缓冲能力进行了比较,得出以下结论:在β-缓冲曲线上显示的每个峰的面积大小和最大pH值表明酸水解的程度、主要氨基酸以及几种寡肽和水溶性蛋白质所给予的丰满程度和口感的延伸程度。这些发现表明,β-缓冲容量反映了电解质的化学成分和提取物的口感质量。
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引用次数: 1
Effects of sorbitol on gelation and cross-linking of myosin heavy chain of salt-ground meat from walleye pollack 山梨糖醇对明太鱼盐磨肉肌球蛋白重链胶凝和交联的影响
Pub Date : 1993-01-01 DOI: 10.2331/SUISAN.59.1599
Y. Funatsu, Hajime Hosokawa, S. Nanbu, K. Arai
Walleye pollack surimi without any additives was ground with 2.5% NaCl, 0.3% sodium polyphosphate, and 0 ?? 8% sorbitol. The salt-ground meat was incubated at 20°C and 40°C for setting, and again at 90°C for 20min to prepare setting-heating gel. Breaking strength (BS) and breaking strain (bs) of the setting and setting-heating gels with the cross-linking of myosin heavy chain (HC) were investigated as a function of setting time.The values of BS and bs of the gels formed through setting with 0.8% sorbitol were higher than those of the gels formed by setting without sorbitol.During the setting at 20°C, cross-linked myosin HCs with small molecular sizes in the gels with 8% sorbitol were formed in a large quantity as compared with those in the gels without sorbitol. However, in the setting at 40°C, the increase of cross-linked myosin HC in the gels with 8% sorbitol was in a small quantity and was nearly identical to that of the gel without sorbitol.These results indicated that sorbitol in surimi affected the cross-linking of myosin HCs of the salt-ground meat and contributed to an improvement in gel formation of the meat.
用2.5% NaCl、0.3%聚磷酸钠、0 ??8%的山梨糖醇。将盐肉末分别于20℃和40℃孵育凝固,再于90℃孵育20min制备凝固加热凝胶。研究了具有肌球蛋白重链交联的凝固和加热凝胶的断裂强度(BS)和断裂应变(BS)随凝固时间的变化规律。添加0.8%山梨醇的凝胶的BS和BS值均高于不添加山梨醇的凝胶。在20℃的凝固过程中,与不含山梨醇的凝胶相比,含有8%山梨醇的凝胶中形成了大量的小分子交联肌球蛋白hc。然而,在40°C的设置下,8%山梨醇凝胶中交联肌球蛋白HC的增加量很少,与不含山梨醇凝胶的增加量几乎相同。这些结果表明,鱼糜中的山梨糖醇影响了盐磨肉中肌球蛋白hc的交联,并有助于改善肉的凝胶形成。
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引用次数: 15
n-3 Polyunsaturated fatty acids in lipids of ascidian Halocynthia roretzi 海鞘脂质中的n-3多不饱和脂肪酸
Pub Date : 1992-11-10 DOI: 10.2331/SUISAN.58.953
M. V. Vysotskii, T. Ota, T. Takagi
The fatty acid composition of the edible ascidain Halocynthia roretzi was studied by means of open-tubular GLC with medium polarity phase Silar-5CP. About fifty components were ob-served, and their distribution among neutral and polar lipids was compared. The main fatty acids were found to be 14:0, 16:0, 16:1 (n-7), 18:0, 18:1 (n-9), 18:1 (n-7), 18:4 (n-3), 20:3 (n-3), 20:5 (n-3), and 22:6 (n-3). In monoenes, (n-7) series fatty acids were predominant, while nine tenths of the polyenoic fatty acids were represented by (n-3) series fatty acids, mainly eicosapen-taenoic. The literature data obtained by packed column GLC were compared with those obtained in the present investigation.
采用中极性相Silar-5CP的开管GLC法研究了食用酸盐的脂肪酸组成。观察了大约50种成分,比较了它们在中性脂和极性脂中的分布。主要脂肪酸为14:0、16:0、16:1 (n-7)、18:0、18:1 (n-9)、18:1 (n-7)、18:4 (n-3)、20:3 (n-3)、20:5 (n-3)和22:6 (n-3)。单烯类脂肪酸以(n-7)系列脂肪酸为主,而十分之九的多烯类脂肪酸以(n-3)系列脂肪酸为主,主要为二十碳烯-二烯类脂肪酸。将填充柱GLC法所得的文献数据与本研究所得的进行了比较。
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引用次数: 6
Estimating rate of gene-centromere recombination at some isozyme loci in Haliotis diversicolor diversicolor and H. discus hannai 异色盘鱼和盘鱼同工酶位点基因-着丝粒重组率的估计
Pub Date : 1992-04-24 DOI: 10.2331/SUISAN.58.445
K. Fujino, K. Arai, M. Kudo, Kuniyuki Takahashi, T. Uematsu, S. Okumura
Analyses on the genotypic compositions in parents and their offspring populations induced by genome manipulation have made it possible to estimate rates of gene-centromere recombination at the four loci in Haliotis diversicolor diversicolor and at the ten loci in H. discus hannai. The present paper presents the results of the similar analyses on genotypic parentages to estimate the rates at the additional four loci in H. diversicolor diversicolor and that at additional one locus in H. discus hannai. Comparisons of the rate at each of seven homologous loci between the two species indicated that the estimates were close each other at the four loci coding phosphoglucomutase, leucine-aminopeptidase, malic enzyme, and superoxide dismutase and that those in H. discus hannai revealed approximately two times greater than those in H. diversicolor diversicolor at the three loci coding phosphoglucose dehydrogenase, phosphoglucose isomerase and mannosephosphate isomerase.
通过基因组操作对亲本及其后代群体基因型组成的分析,可以估算出在4个基因座和10个基因座上的着丝粒重组率。本文采用相似的基因型亲本分析方法,估计了多色麻的4个基因座和多色麻的1个基因座的遗传率。对7个同源基因座进行比较,结果表明,在编码磷酸葡萄糖糖化酶、亮氨酸氨基肽酶、苹果酸酶和超氧化物歧化酶的4个基因座上,两种植物的估计接近,而在编码磷酸葡萄糖脱氢酶、磷酸葡萄糖异构体酶和甘露糖磷酸异构体酶的3个基因座上,两种植物的估计约为两倍。
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引用次数: 3
Pharmacokinetic study of oxytetracycline in cultured rainbow trout, amago salmon, and yellowtail 土霉素在虹鳟、阿玛哥鲑鱼和黄尾鱼体内的药动学研究
Pub Date : 1992-01-01 DOI: 10.2331/SUISAN.58.1151
K. Uno, T. Aoki, R. Ueno
The pharmacokinetics and tissue levels of an antibiotic, oxytetracycline, were determined after oral administration (100mg/kg body weight) in cultured rainbow trout, amago salmon, and yel-lowtail. Oxytetracycline levels were determined by high-performance liquid chromatography. Serum levels of oxytetracycline in three fish species could not be fitted to compartment pharmaco-kinetic models with first-order absorption. The area under the serum concentration-time curve (AUC) were 32.1, 58.7, and 38.4 (μg •h/ml) for rainbow trout, amago salmon, and yellowtail, re-spectively. The mean residence time calculated from AUC were 50.3, 24.6, and 43.3h for rainbow trout, amago salmon, and yellowtail, respectively. Biological half-lives (T1/2) of the serum were 23, 16, and 28h for rainbow trout, amago salmon, and yellowtail, respectively. The T1/2 of muscle in yellowtail was shorter than those of the two salmonids. Oxytetracycline residues persisted in the liver of the three fish species. Elimination times were 22, 21, and 21 days for rainbow trout, amago salmon, and yellowtail, respectively.
口服抗生素土霉素(100mg/kg体重)对养殖虹鳟鱼、阿玛古鲑鱼和黄尾鱼的药代动力学和组织水平进行了测定。高效液相色谱法测定土霉素的含量。三种鱼类的血清土霉素水平不能符合一级吸收的室药动学模型。虹鳟鱼、阿玛戈鲑鱼和黄尾鱼的血清浓度-时间曲线下面积分别为32.1、58.7和38.4 (μg•h/ml)。根据AUC计算的平均停留时间,虹鳟鱼、阿玛戈鲑鱼和黄尾鱼分别为50.3、24.6和43.3h。虹鳟鱼、阿玛戈鲑鱼和黄尾鱼血清的生物半衰期(T1/2)分别为23、16和28h。黄尾鱼肌肉的T1/2比两种鲑科鱼短。土霉素残留在三种鱼类的肝脏中。虹鳟鱼、阿玛戈鲑鱼和黄尾鱼的清除时间分别为22、21和21天。
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引用次数: 29
Non-protein nitrogenous compounds in muscle extract of oceanic cephalopods 海洋头足类动物肌肉提取物中的非蛋白含氮化合物
Pub Date : 1992-01-01 DOI: 10.2331/SUISAN.58.2383
H. Iida, Koji Nakamura, T. Tokunaga
Chemical compositions, especially non-protein nitrogenous constituents of extractives from 14 kinds of squid and octopus caught in far seas, were analyzed to research the possibility of utilization as human food. The constituent of the extractive nitrogenous compounds was markedly different depending on the species. The ammonia content in muscle varied widely depending on the kind of squid. The highest value, 756mg-N/100g, was found in muchi-ika Mastigoteuthis sp., followed by yurei-ika Chiroteuthis imperator and yatsude-ika Octopoteuthis sp., which can be called true ammoniaic squid. In addition to ammonia, large amounts of TMA-N and DMA-N were deter-mined in several kinds of squid such as hotaru-ika-modoki Enoploteuthis chunu, suji-ika Eucleo-teuthis luminosa, and muchi-ika. In very large squid, giant squid Architeuthis japonica and nyudo-ika Moroteuthis robusta, extremely high moisture of around 90% was found and a very high level of ammonia was also detected. It is confirmed that the ammonia in squid muscle exists as ammonium chloride, because the concentration of chloride ion was almost equivalent to that of ammonium ion in all spec-imens analyzed. Composition and concentration of free amino acids varied also depending on the species. Generally, the concentration of free amino acids in oceanic squid was lower than that in normal squid. Considering the chemical characteristics of ammoniaic squid, it may be difficult to use them as human food.
分析了14种远海鱿鱼和章鱼提取物的化学成分,特别是非蛋白氮成分,探讨了其作为人类食物的可能性。不同种属提取的含氮化合物成分有显著差异。肌肉中的氨含量因鱿鱼种类而异。其含量最高的是muchi-ika Mastigoteuthis sp.,为756mg-N/100g,其次是yurei-ika Chiroteuthis imperator和yatsude-ika Octopoteuthis sp.,可称为真氨态鱿鱼。除氨外,还在hotaru- ka-modoki Enoploteuthis chunu、suji-ika Eucleo-teuthis luminosa和muchi-ika等几种鱿鱼中检测到大量的TMA-N和DMA-N。在非常大的鱿鱼,巨型鱿鱼Architeuthis japonica和nyudo-ika Moroteuthis robusta中,发现了大约90%的极高水分,并且还检测到非常高的氨含量。鱿鱼肌肉中的氨以氯化铵的形式存在,因为所有样品中氯离子的浓度与铵离子的浓度几乎相等。游离氨基酸的组成和浓度也因物种而异。一般来说,海洋鱿鱼的游离氨基酸浓度低于正常鱿鱼。考虑到氨鱿鱼的化学特性,可能很难将其作为人类的食物。
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引用次数: 11
Breeding season of the sunray surf clam Mactra chinensis in Tomakomai, southwest Hokkaido [Japan] 北海道西南部Tomakomai海蚬的繁殖期
Pub Date : 1992-01-01 DOI: 10.2331/SUISAN.58.1279
I. Sakurai, M. Kurata, T. Miyamoto
The annual reproductive cycle, breeding season and shell length at first sexual maturity of the sunray surf clam, Mactra chinensis, in Tomakomai, were examined histologically. Devel-opment of the germ cells was divided into five stages; in the female, they were oogonium, yolkless stage, early yolk-formation, late yolk-formation, and mature stage; in the male, they were spermatogonium, primary spermatocyte, secondary spermatocyte, spermatid, and sper-matozoon. On the basis of cytological characteristics and the relative amount of germ cells, maturation of the gonad was classified into recovery, early growing, late growing, maturing, spawning, and spent stages. The early growing stage appeared in March, while the late grow-ing stage was found in April in the male and in May in the female. The maturing stage was observed from May to June in the male and in June in the female, while the spawning stage occurred from July to September. The spent and recovery stages appeared from Octo-ber to November and from December to February, respectively. The breeding season of the clam was estimated to be from July to September. Shell length at first sexual maturity was 25-30mm.
用组织学方法对Tomakomai地区的中国蛤(Mactra chinensis)的年繁殖周期、繁殖季节和初次性成熟时的壳长进行了研究。生殖细胞的发育分为五个阶段;雌性分别为卵母细胞期、无蛋黄期、卵黄形成早期、卵黄形成晚期和成熟期;在男性中,它们是精原细胞、初级精母细胞、次级精母细胞、精细胞和精母细胞。根据性腺的细胞学特征和生殖细胞的相对数量,将性腺成熟分为恢复期、生长早期期、生长晚期期、成熟期、产卵期和度过期。雄性和雌性分别在4月和5月进入生育期,雄性和雌性分别在3月和4月进入生育期。雄鱼成熟期为5 ~ 6月,雌鱼成熟期为6月,产卵期为7 ~ 9月。10 - 11月为消耗期,12 - 2月为恢复期。据估计,蛤的繁殖期为7月至9月。初性成熟时壳长25 ~ 30mm。
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引用次数: 3
Discarding of the Japanese mantis shrimp Oratosquilla oratoria by small-scale trawlers in Tokyo bay [Japan]. 东京湾小型拖网渔船丢弃的日本螳螂虾(Oratosquilla oratoria)。
Pub Date : 1992-01-01 DOI: 10.2331/SUISAN.58.665
J. Ohtomi, N. Nakata, M. Shimizu
In Tokyo Bay small-scale trawlers discard considerable percentages of their catch of the Japanese mantis shrimp Oratosquilla oratoria, but no quantitative survey has so far been carried out. We aimed at making clear the actual fishing mortality, performed a monthly survey on discard percentages from April 1989 to April 1990 to discover seasonal changes, and estimated mortality rate after discarding through simultaneous on board experiments. The results revealed that both the discard percentage and the mortality of the discarded individuals are highest in summer, which was considered to be caused mainly by high air temperature. In total, individuals 2.4 times as large as those landed were discarded and died in a year.
在东京湾,小型拖网渔船丢弃了相当比例的日本螳螂虾(Oratosquilla oratoria),但迄今尚未进行定量调查。为了弄清实际的捕捞死亡率,我们从1989年4月至1990年4月每月进行一次弃置百分比调查,以发现季节变化,并通过船上同步实验估计弃置后的死亡率。结果表明,夏季丢弃率和丢弃个体死亡率最高,主要原因是气温较高。总共有2.4倍大的个体在一年内被丢弃并死亡。
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引用次数: 13
期刊
Bulletin of the Japanese Society of Scientific Fisheries
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