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Perceptions of pesticide use and health risks among apple growers in Kashmir Himalaya 克什米尔喜马拉雅地区苹果种植者对杀虫剂使用和健康风险的认知
Pub Date : 2023-07-13 DOI: 10.1002/jsf2.143
Moieza Ashraf, Saima Hassan, Shahid Ahmad Dar

Background

Understanding the patterns of pesticide use is important in protecting the environment and farmer's health for improvement of sustainability in chemical pest control. Studies on the knowledge and awareness of pesticides by farmers are often incomplete. Therefore, our study aimed at investigating the awareness and knowledge of pesticides from a detailed survey of 394 apple growers in the Kashmir Himalaya.

Results

The study revealed that farmers have inadequate knowledge and awareness about pesticides. About 47.2% of the farmers are choosing pesticides based on the efficiency of the pest/pathogen control, 38.1% are choosing pesticides based on the cost of the product, and 14.7% of the farmers are choosing pesticides based on ease of availability. Most of the farmers rated the principles of Integrated Pest Management (IPM) as not important. The farmers believed that the use of pesticides is the only way of controlling the pest outbreaks that ultimately led to reckless spraying of pesticides. Our results revealed that farmers are spraying pesticides without protective gears and inappropriate dumping of pesticides and their containers, risking the health of humans and the environment. Almost 96% of the farmers feel burning and irritation to the eyes and skin, headache, and vomiting due to pesticide spraying. The results of this research work accentuate the requirement to educate apple growers about the adequate and safe use of pesticides to avoid risks to public and environmental health.

Conclusion

The study concludes that farmers are significantly impacted by the use of pesticides. Ineffective actions of pesticide dealers, government regulations, and illiteracy of farmers are the main causes of the reckless use of pesticides. Therefore, special attention should be paid towards sustainable approaches of pesticide spraying and use of safety gears like personal protective equipment, for protection of human and environmental health.

背景了解农药使用模式对于保护环境和农民健康,提高化学害虫防治的可持续性具有重要意义。农民对农药知识和认识的研究往往是不完整的。因此,我们的研究旨在调查克什米尔喜马拉雅地区394名苹果种植者对杀虫剂的认识和知识。结果研究表明,农民对农药的认识和认识不足。约47.2%的农民根据病虫害/病原体控制的效率选择农药,38.1%的农民根据产品成本选择农药,14.7%的农民基于易得性选择农药。大多数农民认为虫害综合治理原则并不重要。农民们认为,使用杀虫剂是控制害虫爆发的唯一方法,而害虫爆发最终导致了不计后果地喷洒杀虫剂。我们的研究结果显示,农民在没有防护装备的情况下喷洒农药,并不当倾倒农药及其容器,这危及人类健康和环境。近96%的农民因喷洒农药而感到眼睛和皮肤灼热、刺激、头痛和呕吐。这项研究工作的结果强调了对苹果种植者进行充分和安全使用杀虫剂教育的要求,以避免对公众和环境健康造成风险。结论农药使用对农民影响较大。农药经销商的无效行为、政府法规和农民的文盲是鲁莽使用农药的主要原因。因此,应特别注意可持续的杀虫剂喷洒方法和个人防护设备等安全装备的使用,以保护人类和环境健康。
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引用次数: 0
Perceptions of pesticide use and health risks among Apple growers in Kashmir Himalayas 克什米尔喜马拉雅山脉苹果种植者对农药使用和健康风险的认知
Pub Date : 2023-07-13 DOI: 10.1002/jsf2.143
M. Ashraf, S. Hassan, S. Dar
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引用次数: 0
Improving functional properties of rice protein isolates by hydrolysis using alcalase enzyme and assessing bioactivities of hydrolysates alcalase酶水解提高水稻分离蛋白的功能特性及其生物活性评价
Pub Date : 2023-07-13 DOI: 10.1002/jsf2.140
Kavitha Susan Issac, Shubha Banerjee, Sahayog N. Jamdar

Background

Developing value-added products from rice grains is becoming increasingly significant to enhance productivity from excess rice grains. Therefore, the objective of this study was to improve the functional properties of rice protein from four rice varieties, namely, RRF-127, RRF-105, Swarna, and Protezin by enzymatic hydrolysis, and also to evaluate bioactivities like antioxidant, ACE, and DPP-IV inhibitory activities of the hydrolysates.

Results

The alcalase hydrolysis increased protein solubility to >80% in the pH range of 4–10. The foaming capacity and stability of rice protein hydrolysates (RPHs) improved with the increase in pH. The hydrolysis significantly improved the emulsification activity index (EAI) in the pH range of 5–9. The hydrolysates showed antioxidant activity, namely, ABTS+radical scavenging, reducing power, and metal ion chelation. RRF-105 was found to possess the most potent antioxidant capacity among the hydrolysates. RPH could alleviate oxidative damage in erythrocytes, and their protective effects were concentration-dependent. The hydrolysates had stronger inhibition of ACE and DPP-IV enzymes. Protezin exhibited the highest ACE inhibition (63%), while Swarna demonstrated the most effective DPP-IV inhibition (66%).

Conclusion

Hydrolysis with alcalase improved the functional and bioactive properties of rice proteins, indicating that RPH could serve as a source of bioactive peptides or as a functional ingredient with potential properties.

利用过剩的稻谷提高生产力,开发稻谷增值产品变得越来越重要。因此,本研究的目的是通过酶解提高4个水稻品种(RRF-127、RRF-105、Swarna和Protezin)水稻蛋白的功能特性,并评价其水解产物的抗氧化、ACE和DPP-IV抑制活性等生物活性。结果在4 ~ 10的pH范围内,alcalase水解使蛋白溶解度提高到80%。随着pH值的增加,大米蛋白水解物(RPHs)的发泡能力和稳定性得到改善,在pH值为5 ~ 9的范围内,大米蛋白水解物的乳化活性指数(EAI)显著提高。水解产物具有抗氧化活性,即清除ABTS•+自由基、还原能力和金属离子螯合能力。结果表明,RRF-105具有较强的抗氧化能力。RPH能减轻红细胞氧化损伤,其保护作用呈浓度依赖性。水解产物对ACE和DPP-IV酶有较强的抑制作用。Protezin表现出最高的ACE抑制(63%),而Swarna表现出最有效的DPP-IV抑制(66%)。结论alcalase水解提高了水稻蛋白的功能和生物活性,表明RPH可以作为生物活性肽的来源或作为具有潜在功能的功能成分。
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引用次数: 0
Effects of different extraction methods on the phenolic profile, antioxidant and antimicrobial activity of the coffee grounds and coffee silverskin (Coffea arabica L.) 不同提取方法对咖啡渣和咖啡银皮酚类物质、抗氧化和抗菌活性的影响
Pub Date : 2023-06-23 DOI: 10.1002/jsf2.139
Heloisa Patrício Inácio, Gabriela Soster Santetti, Marina Volpato Dacoreggio, Isabel Cristina da Silva Haas, Julia Baranzelli, Tatiana Emanuelli, Rodrigo Barcellos Hoff, Aniela Pinto Kempka, Carlise Beddin Fritzen Freire, Renata Dias de Mello Castanho Amboni

Background

During coffee processing, a large volume of waste is generated. This study investigated the phenolic profile, antioxidant and antibacterial activity of coffee grounds and silverskin using different extraction methods.

Results

Ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), and ultrasound-assisted enzyme extraction (UAEE) were effective for extraction of polyphenols, with UAE being the method that promoted the highest extraction of polyphenols. The coffee ground and coffee silverskin extracts showed high antioxidant capacity for the three methods ABTS, DPPH, and ORAC (4013.36–3241.89 μmol Trolox/g; 1.82–2.06 IC50 μg/mL; and 26.60–16.20 μmol Trolox/mL, respectively). The phenolic profile demonstrated that most of the compounds in both matrices were chlorogenic acid, caffeic acid, 2,4-DHBA, 2,5-DHBA, and 3,4-DHB. EAE promoted a greater release of polyphenols in the coffee ground (2676.42 μg/g), while UAEE was more effective for the coffee silverskin (2111.54 μg/g). The Principal Component Analysis grouped the samples according to the extraction method, based on their content and phenolic potential, for coffee ground and coffee silverskin. As for antibacterial activity, the most expressive result was for Gram-positive bacteria. For both matrices, when extracted by the UAEE method, they presented the minimum inhibitory concentration to inhibit the growth of Bacillus subtilis, Bacillus cereus, and Listeria monocytogenes.

Conclusion

The extraction methods used are environmentally safe alternatives, enabling the reuse of natural compounds and contributing to the circular economy, seeking to transform waste into raw material for the extraction of compounds of interest and possible subsequent application in agriculture as biofertilizers or bioherbicides.

在咖啡的加工过程中,会产生大量的废物。研究了不同提取方法下咖啡渣和银皮的酚类成分、抗氧化和抗菌活性。结果超声辅助提取法(UAE)、酶辅助提取法(EAE)和超声辅助酶提取法(UAEE)均可有效提取多酚,其中UAE法对多酚的提取率最高。咖啡渣和咖啡银皮提取物对ABTS、DPPH和ORAC具有较高的抗氧化能力(4013.36 ~ 3241.89 μmol Trolox/g);1.82 ~ 2.06 IC50 μg/mL;和26.60 ~ 16.20 μmol Trolox/mL)。酚类分析表明,两种基质中的大部分化合物为绿原酸、咖啡酸、2,4- dhba、2,5- dhba和3,4- dhb。EAE对咖啡渣中多酚的释放作用更大(2676.42 μg/g),而UAEE对咖啡银皮的释放作用更大(2111.54 μg/g)。主成分分析根据提取方法对咖啡粉和咖啡银皮的含量和酚类电位进行分组。抑菌活性以革兰氏阳性菌最具代表性。对于这两种基质,当用UAEE法提取时,它们都呈现出抑制枯草芽孢杆菌、蜡样芽孢杆菌和单核增生李斯特菌生长的最低抑制浓度。结论所采用的提取方法是一种环境安全的替代方法,可以实现天然化合物的重复利用,促进循环经济,寻求将废物转化为提取感兴趣化合物的原料,并可能在农业上作为生物肥料或生物除草剂应用。
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引用次数: 0
Target-mediated suppression of rolling circle amplification for label-free detection of aflatoxin B1 目标介导的滚动圈扩增抑制黄曲霉毒素B1无标记检测
Pub Date : 2023-06-20 DOI: 10.1002/jsf2.138
Lili Yao, Yuqin Cheng, Baolei Dong, Ling Yan, Hao Qu, Yu Mao, Changhong Liu, Lei Zheng

Background

Aflatoxin B1 (AFB1) has been considered as a prevailing natural carcinogenic mycotoxin in various food and feedstuff products, posing serious endangers to human health. The quantitative and selective detection of AFB1 was significantly essential for food safety control.

Results

We developed a label-free fluorescent aptasensor using a target-binding aptamer probe as the rolling circle amplification (RCA) primer for AFB1 detection. In the presence of AFB1, the probe preferentially combined with AFB1 rather than binding to the circular template, inhibiting the initiation of RCA process. The detection limit of the proposed method was as low as 6.6 nM, and the linear dynamic response range was four orders of magnitude wide. Besides, it revealed excellent specificity for AFB1 and high-quality detection performance in food samples.

Conclusion

The presented detection method was simple, rapid, and economical, which indicated a promising application in food safety monitoring.

黄曲霉毒素B1 (AFB1)被认为是各种食品和饲料产品中普遍存在的天然致癌性真菌毒素,对人类健康造成严重危害。AFB1的定量和选择性检测对食品安全控制具有重要意义。结果我们开发了一种无标记的荧光核酸适体传感器,使用目标结合核酸适体探针作为滚动圈扩增(RCA)引物检测AFB1。在AFB1存在的情况下,探针优先与AFB1结合而不是与圆形模板结合,从而抑制了RCA过程的启动。该方法的检出限低至6.6 nM,线性动态响应范围宽4个数量级。此外,该方法对AFB1具有良好的特异性,在食品样品中具有较高的检测性能。结论该检测方法简便、快速、经济,在食品安全监测中具有广阔的应用前景。
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引用次数: 0
Traditional Asian plant-based fermented foods as vitamin B12 sources: A mini-review 传统亚洲植物发酵食品作为维生素B12来源:综述
Pub Date : 2023-06-20 DOI: 10.1002/jsf2.137
Kyohei Koseki, Fei Teng, Tomohiro Bito, Fumio Watanabe

Plant-based foods, such as vegetables and beans, are deficient in vitamin B12 (B12). If plant-based foods containing adequate B12 can be produced microbially, incorporating them into a vegetarian diet would prevent B12 deficiency. Asian indigenous fermented foods from soybeans, vegetables, and tea leaves, are commercially accessible. The B12 level in the fermented products has been characterized to determine whether they may serve as a viable source of B12 in humans. Since their B12 content is very low (<0.5 μg/100 g wet weight), most traditional Asian plant-based fermented foods do not appear to serve as adequate daily B12 sources. However, some stinky tofu and pickled vegetable products contain >10 μg of B12 per 100 g wet weight. Nonetheless, further clinical research is warranted to clarify whether these products can increase the serum B12 levels of humans.

植物性食物,如蔬菜和豆类,缺乏维生素B12。如果含有足够B12的植物性食物可以通过微生物产生,那么将它们纳入素食中就可以预防B12缺乏症。亚洲本土的大豆、蔬菜和茶叶发酵食品在商业上是可以买到的。已对发酵产品中的B12水平进行了表征,以确定它们是否可以作为人类B12的可行来源。由于它们的B12含量非常低(0.5 μg/100克湿重),大多数传统的亚洲植物发酵食品似乎不能作为足够的日常B12来源。然而,一些臭豆腐和腌制蔬菜产品每100克湿重含有10 μg的B12。然而,需要进一步的临床研究来阐明这些产品是否能提高人类血清B12水平。
{"title":"Traditional Asian plant-based fermented foods as vitamin B12 sources: A mini-review","authors":"Kyohei Koseki,&nbsp;Fei Teng,&nbsp;Tomohiro Bito,&nbsp;Fumio Watanabe","doi":"10.1002/jsf2.137","DOIUrl":"10.1002/jsf2.137","url":null,"abstract":"<p>Plant-based foods, such as vegetables and beans, are deficient in vitamin B<sub>12</sub> (B<sub>12</sub>). If plant-based foods containing adequate B<sub>12</sub> can be produced microbially, incorporating them into a vegetarian diet would prevent B<sub>12</sub> deficiency. Asian indigenous fermented foods from soybeans, vegetables, and tea leaves, are commercially accessible. The B<sub>12</sub> level in the fermented products has been characterized to determine whether they may serve as a viable source of B<sub>12</sub> in humans. Since their B<sub>12</sub> content is very low (&lt;0.5 μg/100 g wet weight), most traditional Asian plant-based fermented foods do not appear to serve as adequate daily B<sub>12</sub> sources. However, some stinky tofu and pickled vegetable products contain &gt;10 μg of B<sub>12</sub> per 100 g wet weight. Nonetheless, further clinical research is warranted to clarify whether these products can increase the serum B<sub>12</sub> levels of humans.</p>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 7","pages":"294-298"},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41390128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid and simultaneous identification of Lonicera Japonicae Flos and Lonicerae Flos using MLPA-HRM and rhPCR-HRM MLPA - HRM和rhPCR - HRM快速同时鉴定金银花和金银花
Pub Date : 2023-06-15 DOI: 10.1002/jsf2.136
Jing Mo, Wenbin Wang, Yongyi Wang, Huachun Cheng, Xiaofang Li, Bo Wang

Background

Lonicera Japonicae Flos (LJF) and Lonicerae Flos (LF), derived from different species of Lonicera, are two different types of traditional Chinese medicine (TCM) according to their differences in composition and effectiveness. Because of the high price, good effectiveness of the medicinal, and functional values, LJF is often adulterated by LF in the market. Therefore, two single nucleotide polymorphism-based genotyping methods, multiplex ligation-dependent probe amplification high-resolution melting (MLPA-HRM) and RNase H2-dependant PCR (rhPCR)-HRM, were established for the identification of LF and LJF.

Results

Highly specific MLPA probes and rhPCR-primers were designed based on the trnL-trnF regions. The melting temperature (Tm) of amplicons was derived from the peak of the HRM curve, and the differences in Tm values were used to identify LJF and LF. The two methods have both shown good specificity for identifying LJF and LF without cross-reaction, and have high sensitivity with the detection limits of 0.1 ng DNA template. For mixed samples, MLPA-HRM could detect LJF mixed with 10% LF while rhPCR-HRM could detect 5% LF in mixed samples. The MLPA-HRM and rhPCR-HRM were performed on DNA extracted from 54 samples of LJF and LF randomly selected from the medicinal material market, all samples were successfully identified and further verified with DNA barcoding.

Conclusion

MLPA-HRM and rhPCR-HRM methods developed in our study not only provided fast, simple, sensitive, and practical identification of LJF and LF, but also provided technical references for the adulteration identification of other herb-medicines, they are of great significance to the quality control and safe use of plant medicinal materials.

金银花(Lonicera Japonicae Flos, LJF)和金银花(Lonicerae Flos, LF)是两种不同种类的中药,它们的成分和药效不同。由于LJF价格高,药用效果好,功能价值高,市场上经常出现LJF掺假现象。因此,建立了两种基于单核苷酸多态性的基因分型方法,多重连接依赖探针扩增高分辨率熔融(MLPA-HRM)和RNase h2依赖PCR (rhPCR)-HRM,用于鉴定LF和LJF。结果基于trnL-trnF区域设计了高特异性的MLPA探针和rhpcr引物。从HRM曲线的峰值得到扩增子的熔化温度(Tm),并利用Tm值的差异来识别LJF和LF。两种方法鉴定LJF和LF均具有良好的特异性,无交叉反应,灵敏度高,检测限为0.1 ng DNA模板。对于混合样品,MLPA-HRM可以检测到混合10% LF的LJF,而rhPCR-HRM可以检测到混合样品中5% LF。对从药材市场随机抽取的54份LJF和LF样品进行MLPA-HRM和rhPCR-HRM,所有样品均成功鉴定并进一步进行DNA条形码验证。结论本研究建立的MLPA-HRM和rhPCR-HRM方法不仅能快速、简便、灵敏、实用地鉴别黄芪和黄芪,还可为其他中药材的掺假鉴定提供技术参考,对植物药材的质量控制和安全使用具有重要意义。
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引用次数: 0
Applications of curcumin loaded nanoemulsions in photoinactivation of Aspergillus niger spores 姜黄素负载纳米乳液在黑曲霉孢子光活化中的应用
Pub Date : 2023-05-25 DOI: 10.1002/jsf2.134
J. Alejandro Arboleda-Murillo, Andres F. Cañon-Ibarra, Leidy T. Sanchez, Magda I. Pinzon, Cristian C. Villa

Background

Aspergillus niger is one of the most common fungal contaminants of food products, leading to several economic losses on fruit, vegetables and other food products during storage. Over the years, several new techniques have been developed to control fungal growth, including photodynamic inactivation, in which excitation of a photosensitizer in the right wavelength leads to ROS production and microbial death.

Results

On this paper, curcumin was used as a natural photosentitizer against A. niger spores. Nanoemulsions were used in order to encapsulated curcumin and increase its water solubility. Results showed that spores inhibition could reach up to 80% after 3 min of irradiation while values for un-encapsulated curcumin were around 30%.

Conclusion

Results presented show that nanoemulsions could be use to enhance the photoinactivation properties of curcumin, against fungal spores from A. niger. This type of process can be use for food decontamination and preservation.

背景黑曲霉是食品中最常见的真菌污染物之一,在储存过程中给水果、蔬菜等食品带来了巨大的经济损失。多年来,已经开发了几种控制真菌生长的新技术,包括光动力灭活,其中在合适的波长下激发光敏剂会导致ROS的产生和微生物死亡。结果姜黄素是一种天然的抗黄曲霉的光稳定剂。 黑色素孢子。使用纳米乳液来包封姜黄素并增加其水溶性。结果表明,3天后孢子抑制率可达80% 而未包封的姜黄素的值约为30%。结论纳米乳液可提高姜黄素对真菌孢子的光活化性能。 尼日尔。这种类型的工艺可以用于食品净化和保存。
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引用次数: 0
Influence of adaptation length on metabolizable energy assay of maize and barley supplemented with or without glucanase determined by index method in broilers 指数法测定玉米和大麦添加或不添加葡聚糖酶对肉鸡代谢能测定的影响
Pub Date : 2023-05-25 DOI: 10.1002/jsf2.135
S. Veluri, O. Olukosi
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引用次数: 0
Applications of Curcumin Loaded Nanoemulsions in Photoinactivation of A. Niger spores 姜黄素负载纳米乳液在光灭活A.Niger孢子中的应用
Pub Date : 2023-05-25 DOI: 10.1002/jsf2.134
J. A. Arboleda‐Murillo, Andrés F. Cañon-Ibarra, Leidy T. Sanchez, M. I. Pinzón, Cristian C. Villa
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引用次数: 0
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