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Ultrasonographic characteristics of prostate and seminal vesicle glands in developing Murrah buffalo bulls 发育中的默拉水牛前列腺和精囊腺的超声特征
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-30 DOI: 10.56825/bufbu.2023.4233507
Lal Chand Ranga, Ramesh Kumar Chandolia, Laxman Singh, Ravi Dutt
The study was performed in Murrah buffalo-bull calves to assess the developmental dimensions of the prostate and seminal vesicle (SV) glands from 1 to 30 months of age with transrectal ultrasonography. The width of prostate gland ranged between 1.39±0.07 and 2.65±0.14 cm. The rate of change in the mean width of the gland was gradual and almost same from one to thirty month (P<0.05). The correlation co-efficient between width of prostate gland and age was r2 = 0.73. The mean circumference and length of the SV gland at one month to thirty months of age ranged from 6.14±0.32 to 13.05±0.34 cm and 2.61±0.09 to 6.02±0.27 cm, respectively. The correlation co-efficient between length and age, width and age, and circumference of SV gland and age were r2 = 0.68, r2 = 0.55 and r2 = 0.74, respectively. From this study, it is concluded that the ultrasonographic evaluation of prostate and SV along with growth rate could be very practical tool for the assessment of puberty.
本研究采用经直肠超声检查法对1 ~ 30月龄Murrah水牛犊牛的前列腺和精囊腺(SV)的发育尺寸进行了评估。前列腺宽度在1.39±0.07 ~ 2.65±0.14 cm之间。腺体平均宽度的变化率是渐进的,从1个月到30个月几乎相同(P<0.05)。前列腺宽度与年龄的相关系数r2 = 0.73。月龄1 ~ 30月龄时,SV腺的平均周长和长度分别为6.14±0.32 ~ 13.05±0.34 cm和2.61±0.09 ~ 6.02±0.27 cm。SV腺长度与年龄、宽度与年龄、周长与年龄的相关系数分别为r2 = 0.68、r2 = 0.55和r2 = 0.74。由此可见,超声检查前列腺及SV随生长速率的变化可作为评估青春期发育的实用工具。
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引用次数: 0
Polypropylene mesh versus nylon mosquito net mesh for external abdominal hernias repair in cattle and buffaloes 聚丙烯网与尼龙蚊帐网在牛和水牛腹外疝修补中的比较
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-30 DOI: 10.56825/bufbu.2023.4233546
Naveen Kumar, Vineet Kumar, Harendra Singh Rathore, Sanjay Purohit, Anil Kumar Gangwar, Swapan Kumar Maiti
Herein, the effectiveness of locally sourced and autoclaved nylon mosquito net mesh for the repair of external abdominal hernias was investigated. Eleven animals (7 cattle and 4 buffaloes) having hernial ring sizes of 8 to 30 cm in diameter were divided into two Groups 1 (5 animals) and Group 2 (6 animals). In Group 1, nylon mosquito net mesh was used, and in Group 2, polypropylene mesh was used for the repair of defects. The animals were sedated with xylazine HCl and given local anaesthesia during surgery. Over the external hernial sac, an elliptical skin incision was made. The internal hernial sac was bluntly dissected and freed down to the hernial ring. The internal hernial sac was opened, and the hernial ring was repaired with nylon mosquito net mesh or polypropylene mesh using the inlay technique. After hernioplasty, the sac and subcutaneous tissue were repaired with prolene suture material. The skin incision was closed with simple interrupted mattress sutures using monofilament polyamide suture material. Antibiotics and analgesics were given postoperatively, and povidone iodine was used to treat the suture line daily. The skin sutures were removed on the 10th to 15th postoperative day, depending upon the wound condition. Mild inflammatory swelling in Group 1 was observed at the incision line, which had completely subsided by the 15th postoperative day. The wounds healed by first intention in both groups. In any case, no recurrence was seen in a follow-up period of 3 months. Without clinical signs of wound dehiscence, infection, or recurrence, all animals recovered uneventfully.
在此,我们研究了局部来源和蒸压尼龙蚊帐网用于腹外疝修复的有效性。将疝环直径为8 ~ 30 cm的动物11只(牛7只、水牛4只)分为1组(5只)和2组(6只)。第1组采用尼龙蚊帐网,第2组采用聚丙烯网进行缺损修复。手术中给予盐酸噻嗪镇静和局部麻醉。在外疝囊上方,做一个椭圆形皮肤切口。直接切开内部疝囊,释放至疝环。打开内疝囊,采用尼龙蚊帐网或聚丙烯网嵌体技术修复疝环。疝成形术后,气囊和皮下组织用丙烯缝合材料修复。皮肤切口采用单丝聚酰胺缝合材料进行简单间断床垫缝合。术后给予抗生素和镇痛药,每日应用聚维酮碘治疗缝合线。根据创面情况,于术后第10 ~ 15天拆除皮肤缝合线。第1组在切口线上出现轻度炎症性肿胀,至术后第15天完全消退。两组创面均首次愈合。随访3个月均未见复发。所有动物均无伤口裂开、感染或复发的临床症状。
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引用次数: 0
Comparative therapeutic efficacy of hypertonic saline solution andisotonic normal saline for fore-stomach impaction disorders in buffaloes (Bubalus bubalis) 高渗生理盐水与强直生理盐水治疗水牛前胃嵌塞症的疗效比较
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-30 DOI: 10.56825/bufbu.2023.4233533
Sujata Turkar, Sanjeev Kumar Uppal, Charanjeet Singh Randhawa
The aim of present investigation was to evaluate the comparative therapeutic efficacy of isotonic (0.9%) saline solution (NSS) and hypertonic (7.5%) saline solution (HSS) along with supportive medical treatment in buffaloes suffered with forestomach impaction. A total of 26 buffaloes were undertaken in two groups. In Group 1, twenty buffaloes were infused with isotonic (0.9%) saline solution 20 ml/kg body weight and in Group 2 six buffaloes were infused with hypertonic saline solution (7.5%) 2 ml/kg. The physical parameters (heart rate and respiration rate) were decreased more significantly in Group 1 after treatment. Similar trend was observed in the pretreatment hematological parameters viz. Hb, PCV, TLC, neutrophil count and ESR after fluid therapy which was indicative of expansion of plasma volume. There was not much effect on rumen liquor parameters after fluid therapy. The biochemical profile (Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), bilirubin, Blood Urea Nitrogen (BUN), creatinine, total protein, glucose, globulin and fibrinogen) was more significantly declined in Group 1 after fluid therapy, whereas plasma sodium and chloride levels significantly increased, and plasma potassium level more significantly decreased in Group 2. The recovery rates were 60% and 17% respectively in Group 1 and Group 2. Thus, Isotonic (0.9%) saline solution appears to more effective in therapeutic management of forestomach impaction in buffaloes.
本研究的目的是比较等渗盐水(0.9%)和高渗盐水(7.5%)配合支持治疗对水牛前胃嵌塞的疗效。共有26头水牛被分为两组。1组20头灌胃等渗(0.9%)生理盐水20 ml/kg体重,2组6头灌胃高渗(7.5%)生理盐水2 ml/kg。治疗后1组患者心率、呼吸速率等生理参数下降更为明显。液体治疗后的血液学参数Hb、PCV、TLC、中性粒细胞计数和ESR也有类似的趋势,表明血浆容量增加。液体疗法对瘤胃液参数影响不大。血浆生化指标(谷草转氨酶(AST)、碱性磷酸酶(ALP)、胆红素、血尿素氮(BUN)、肌酐、总蛋白、葡萄糖、球蛋白和纤维蛋白原)在液体治疗后1组下降更为显著,血浆钠、氯水平显著升高,血浆钾水平显著降低。组1和组2的回收率分别为60%和17%。因此,等渗(0.9%)生理盐水溶液在治疗水牛前胃嵌塞方面似乎更有效。
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引用次数: 0
Test-day and other milk recording options for prediction of lactation milk yield in Jaffarabadi (Bubalus bubalis) buffaloes Jaffarabadi (Bubalus bubalis)水牛泌乳量预测的测试日和其他牛奶记录选项
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-30 DOI: 10.56825/bufbu.2023.4234646
Pravin Nagibhai Chaudhari, Pratik Sanjaybhai Kapadiya, Mahesh Ramnivas Gadariya, Pranav Mayankbhai Gamit, Bhagavanji Dayabhai Savaliya
Generally, standard lactation milk yield is predicted based on test-day records collected at monthly intervals. Test-day milk production at different time intervals other than monthly intervals can be used to predict lactation milk yield of field bovines in field conditions. With the same possibility, this study was carried out to predict lactation milk yield in Jaffarabadi buffaloes from various test-day milk yield data retrieved for different time intervals. A total of 1,15,339 daily milk yield records in 176 lactations of 1st to 6th parity of 30 Jaffarabadi buffaloes lactating at the Cattle Breeding Farm, Kamdhenu University, Junagadh, Gujarat over a period of 28 years (1991 to 2018) were used for the study. Single monthly test-day milk yield recorded on 125th, 155th or 185th day i.e., 5th, 6th and 7th monthly test day yield alone provided only 50% reliability in determining the standard lactation milk yield. Daily peak yield alone was also found to be a poor predictor for lactation yield. Prediction equations using combination of consecutive two monthly test day yields from 4th to 10th monthly test day were found reliable source for prediction of lactation milk yield providing accuracy up to 82.19% whereas, daily peak yield in combination with single monthly test day yield at mid and late lactation was also predicted lactation milk yield with accuracies up to 72.23%. Milk production recorded at weekly interval could also be used to approximate milk production using the equation 15.35+6.91 × Sum of all weekly test-day yields, with precision of 98.93% or milk production recorded at fortnightly interval by the equation 18.04+14.65 × Sum of fortnightly test-day yields, with precision of 97.14%.
一般来说,标准泌乳量是根据每月收集的测试日记录来预测的。试验日产奶量在不同的时间间隔,而不是每月间隔,可用于预测奶牛在田间条件下的泌乳量。基于同样的可能性,本研究通过不同时间间隔的不同测试日产奶量数据来预测贾法拉巴迪水牛的泌乳量。该研究使用了古吉拉特邦朱纳加德坎德努大学养牛场30头Jaffarabadi水牛在28年(1991年至2018年)期间的176次1胎至6胎哺乳的1,15,339次日产奶量记录。在第125、155或185天,即第5、6和7个月测试日单独记录的单月测试日产奶量在确定标准泌乳产奶量时仅提供50%的可靠性。每日峰值产奶量也被发现是一个较差的预测量。利用连续2个月试验日产奶量(4 ~ 10个月试验日产奶量)组合的预测方程预测泌乳量的准确性可达82.19%,而每日峰值产奶量与泌乳中后期单月试验日产奶量组合预测泌乳量的准确性也可达72.23%。以周为间隔记录的产奶量也可用公式15.35+6.91 ×所有周试验日产奶量之和近似产奶量,精度为98.93%;或以两周为间隔记录的产奶量用公式18.04+14.65 ×两周试验日产奶量之和近似产奶量,精度为97.14%。
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引用次数: 0
Ultrasonographic diagnosis of reproductive disorders in Murrah buffaloes 穆拉水牛生殖障碍的超声诊断
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-30 DOI: 10.56825/bufbu.2023.4233225
Ravi Dutt, Gyan Singh
The present investigation was carried out in the year 2016 to 2017 at government livestock farm sector-1 and buffalo farm of LUVAS on Murrah buffaloes and a total of 70 post-partum animals were examined using real time B mode ultrasonography. A total of 13 animals were diagnosed with reproductive ailments including granulosa cell tumour, follicular cyst, mucometra, parovarian cyst, pyometra, hydrometra, cystitis and early embryonic mortality
本研究于2016 - 2017年在LUVAS政府畜牧场1部门和水牛养殖场对Murrah水牛进行了调查,共70只产后动物进行了实时B超检查。共有13只动物被诊断患有生殖疾病,包括颗粒细胞瘤、卵泡囊肿、粘液瘤、卵巢旁囊肿、脓水、积水、膀胱炎和早期胚胎死亡
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引用次数: 0
Ultrasonographic assessment of effect of neostigmine on abomasal atony in young calves 超声评价新斯的明对犊牛皱胃张力的影响
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-30 DOI: 10.56825/bufbu.2023.4233968
Bhavanam Sudhakara Reddy, Sirigireddy Sivajothi, Kambala Swetha
Present communication was focused on the ultrasonographic evaluation of the prokinetic effect of neostigmine in buffalo calves with abomasal atony. Six buffalo calves were selected for the study with absence of rumination, loss of feed intake, mild distended abdomen, achezia and frequent history of laying down and getting up with reduced rumen motility. Abomasal atony was confirmed by the ultrasonographic assessment of the abomasum motility. Calves were administered with injection neostigmine 0.02 mg/kg body weight slow intravenously along with 500 ml of 0.9% normal saline under the ultrasonographic monitoring. Abomasal motility was initiated by 18 to 42 minutes and complete abomasal motility was noticed by 150 to 180 minutes after administration of medications. Clinical improvement was noticed on the third day of therapy and efficacy of the neostigmine was noticed by ultrasonography. Present study concludes that neostigmine has prokinetic effect in buffalo calves with abomasal functional motility disorders. Ultrasonography is the best tool for assessment of abomasal motility without any adverse reactions and complications.
本文就新斯的明对皱胃肌张力失调小牛的促动力学作用的超声评价作一综述。选取6头无反刍、采食量减少、轻度腹胀、残缺、频繁起躺、瘤胃运动减少的水牛犊牛进行研究。超声检查证实皱胃肌张力不全。在超声监测下,犊牛缓慢静脉注射新斯的明0.02 mg/kg体重,同时加入0.9%生理盐水500 ml。给药后18 ~ 42分钟开始皱胃运动,150 ~ 180分钟皱胃运动完全。治疗第3天临床改善,超声检查新斯的明疗效。本研究表明,新斯的明对患有皱胃功能性运动障碍的水牛犊牛具有促进运动的作用。超声检查是评估皱胃运动的最佳工具,无任何不良反应和并发症。
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引用次数: 0
Assessing the acceptability of hormone-based estrus induction / synchronization protocols to overcome anestrus in buffaloes 评估以激素为基础的发情诱导/同步方案的可接受性,以克服水牛的不发情
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-30 DOI: 10.56825/bufbu.2023.4233575
Mallikerimath Mahantswamy, Sarvpreet Singh Ghuman, Bilawal Singh
Five hundred dairy households in the rural areas of Punjab state were interviewed through comprehensively designed questionnaires to assess the knowledge and experience of farmers regarding the hormone-based strategies to overcome anestrus and getting the buffaloes pregnant. About 81.8% dairy households were unaware (P<0.05) about any estrus induction or synchronization hormonal protocol and majority (65.5%) of these belonged to households with <5 animals. Out of households having awareness about hormonal protocol use in buffaloes, the majority (58.2%) belonged to households with >15 animal. Out of dairy farmers using hormonal protocols, the proportion of illiterate was 14.8%. Out of dairy farmers not aware about hormonal protocols, the proportion of illiterate was 52.3%. Data analysis of 91 dairy households who had used hormonal protocols revealed summer (65.9%) and anestrus (82%) as the major underlying reasons for their use in buffaloes. Low success rate (20.2%), high cost (50%) and non-availability of doctor (29.7%) were the reasons for the irregular use of hormonal protocols. The success rate of hormonal protocols under field condition in terms of conception was 47.1%. In summary, the major bottlenecks in acceptability of hormone-based therapeutics strategies under filed conditions are illiteracy, high cost of therapeutics strategies and lack of awareness in buffalo farmers especially in households with <5 animals.
通过综合设计的问卷,对旁遮普邦农村地区的500个奶农家庭进行了访谈,以评估农民对以激素为基础的策略克服发情和使水牛怀孕的知识和经验。约81.8%的奶牛家庭不知道任何发情诱导或同步激素方案(P<0.05),其中大多数(65.5%)属于有5头奶牛的家庭。在了解水牛激素使用方案的家庭中,大多数(58.2%)属于拥有15头动物的家庭。在使用激素方案的奶农中,文盲的比例为14.8%。在不了解激素方案的奶农中,文盲比例为52.3%。对91个使用激素方案的奶牛家庭的数据分析显示,夏季(65.9%)和发情(82%)是水牛使用激素的主要潜在原因。不规律使用激素方案的原因有:成功率低(20.2%)、费用高(50%)和无医生(29.7%)。野外条件下激素方案受孕成功率为47.1%。总之,在野外条件下接受激素治疗策略的主要瓶颈是文盲、治疗策略的高成本和水牛农民缺乏认识,特别是在有5只动物的家庭中。
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引用次数: 0
Use of hyaluronidase enzyme for hastening the cervical dilatation in successfully detorted uterine torsion affected buffaloes 应用透明质酸酶加速宫颈扩张在成功扭转子宫扭转水牛中的应用
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-30 DOI: 10.56825/bufbu.2023.4233570
Randhir Singh, Sarvpreet Singh Ghuman, Navdeep Singh
The successfully detorted uterine torsion buffaloes were either subjected to routine post-detorsion treatment (n=10) or in addition to routine treatment, intracervical hyaluronidase enzyme was administered (10,000 IU; 2.5 ml at each of 3, 6, 9, 12 o’clock position of cervix) immediately post-detorsion (n=10), followed by repeated administration at 6h interval (0, 6, 12, 18 h) till complete cervical dilatation or till 24 h post-detorsion. The time elapsed between successful uterine detorsion to complete cervical dilatation was reduced (P<0.05) in buffaloes subjected to enzyme treatment in comparison to their control counterparts (754.6±114.0 vs. 213.5±33.1 minutes). In buffaloes delivering dead fetus, following intracervical treatment, the interval between detorsion to complete cervical dilatation was reduced (P<0.05) to 240.8±44.0 minutes in comparison to their control counterparts (838.1±126.2 minutes). Moreover, following hyaluronidase treatment, the time interval from detorsion of a buffalo to complete cervical dilatation with respect to degree of uterine torsion was much lower (P<0.05) in treated compared to their control group counterparts having either <180o or ≥180o uterine torsion. The fertility parameters (number of days to first estrus and duration of service period) were improved (P>0.05) in hyaluronidase-treated buffaloes of both the groups (<18 h or ≥18 h duration of torsion) as compared to their control counterparts. Conclusion: Intracervical hyaluronidase treatment hastens the cervical dilatation in successfully detorted uterine torsion affected buffaloes with a subsequent potentially beneficial impact on fertility.
成功扭转的子宫扭转水牛,要么进行常规扭转后治疗(n=10),要么在常规治疗的基础上,给予宫颈内透明质酸酶(10000 IU;在宫颈3、6、9、12点钟位置(n=10)立即给药2.5 ml,然后每隔6小时(0、6、12、18小时)重复给药,直至宫颈完全扩张或至宫颈扭转后24小时。与对照组相比,经酶处理的水牛子宫成功扭转至宫颈完全扩张所需的时间(754.6±114.0分钟比213.5±33.1分钟)缩短(P<0.05)。在分娩死胎的水牛中,经过宫颈内治疗,与对照组(838.1±126.2分钟)相比,从扭曲到完全宫颈扩张的时间间隔缩短(P<0.05)至240.8±44.0分钟。此外,在透明质酸酶治疗后,与子宫扭转度为180度或≥180度的对照组相比,治疗组水牛从子宫扭转到完全宫颈扩张的时间间隔(P<0.05)要低得多。透明质酸酶处理的两组水牛(扭转期为18 h或扭转期≥18 h)的生育参数(至首次发情天数和服务时间)均较对照组提高(P>0.05)。结论:宫颈内透明质酸酶治疗可加速成功扭转子宫的水牛的宫颈扩张,对其生育能力有潜在的有益影响。
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引用次数: 0
Effect of some non-genetic factors on productive and reproductive traits of Mehsana buffaloes 一些非遗传因素对梅萨那水牛生产和繁殖性状的影响
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-30 DOI: 10.56825/bufbu.2023.4233965
Rishit Navinchandra Sathwara, Jay Prakash Gupta, Jagdish Devji Chaudhari, Pravin Ambabhai Patel
The records of 7782 Mehsana buffaloes sired by 184 sires maintained at Dudhsagar Research and Development Association (DURDA), Dudhsagar Dairy, Mehsana, Gujarat born during 1989 to 2012 were collected and analysed to examine the effect of non-genetic factors viz. period and season of calving and age at first calving. The least squares analysis was used for estimation of various non-genetic factors on First Lactation Milk Yield (FLMY), First Lactation Fat Yield (FLFY), Average Fat Percentage (AFP) and Age at First Calving (AFC). The least squares mean with standard error of FLMY, FLMY, AFP and AFC were estimated to be 1900.68±7.65 lit., 135.04±0.57 kg, 7.11±0.11 percentage and 1407.18±4.36 days, respectively. Highly significant (P≤0.01) effect of all the non-genetic factors (period and season of calving and age at first alving group) under study was observed on FLMY, FLMY and AFP in Mehsana buffaloes. Further, highly significant (P≤0.01) effect of season and period of birth was recorded on AFC. It is worthy to note that in Mehsana breed evaluation programme these factors should be considered as they have great influence of them.
收集和分析了1989年至2012年期间出生的7782头Mehsana水牛的记录,这些水牛来自于古吉拉特邦的Dudhsagar研究与发展协会(DURDA), Dudhsagar奶牛场,Dudhsagar, Mehsana。采用最小二乘分析对初泌乳产奶量(FLMY)、初泌乳脂肪量(FLFY)、平均脂肪率(AFP)和初产龄(AFC)的非遗传因素进行估计。FLMY、FLMY、AFP和AFC的最小二乘平均标准误差分别为1900.68±7.65 lit.、135.04±0.57 kg、7.11±0.11%和1407.18±4.36 d。各非遗传因素(产犊期、产犊季、初产组龄)对Mehsana水牛FLMY、FLMY和AFP均有极显著(P≤0.01)的影响。季节和出生期对AFC的影响极显著(P≤0.01)。值得注意的是,在马肉鸡品种评价方案中应考虑这些因素,因为它们对马肉鸡品种有很大的影响。
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引用次数: 0
The impact of intracervical hyaluronidase enzyme on cervical histomorphology in successfully detorted uterine torsion affected buffaloes 宫颈内透明质酸酶对成功扭转子宫畸形水牛宫颈组织形态学的影响
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-30 DOI: 10.56825/bufbu.2023.4233577
Randhir Singh, Sarvpreet Singh Ghuman, Devendra Pathak, Navdeep Singh
The present study evaluated the impact of intracervical hyaluronidase enzyme on cervical histomorphology in successfully detorted uterine torsion affected buffaloes. These animals were either subjected to routine post-detorsion treatment (n=10) or in addition to routine treatment, hyaluronidase enzyme (10,000 IU; 2.5 ml at each of 3, 6, 9, 12 o’clock position of cervix) was administered intracervically immediately post-detorsion (n=10), followed by repeated administration at 6 h interval (0, 6, 12, 18 h) till complete cervical dilatation or till 24 h after detorsion. The pre-treatment cervical biopsy samples were collected immediately after detorsion and post-treatment samples were collected at complete cervical dilatation which happened within 24 h after detorsion in all the cases. Following histomorphology, the quantification of collagen fibers as mean percentage area occupied collagen out of total tissue area in pre-treatment cervical biopsy samples of all the buffaloes was revealed as 21.0±5.7. During post-treatment period, at the time of complete cervical dilatation, this value exhibited a decrease in control (9.1±2.2; P>0.05) as well as hyaluronidase group (5.8±2.1; P<0.05). In summary, collagen fiber dispersion in cervical tissue of successfully detorted uterine torsion affected buffaloes suggested their role in cervical dilatation, whereby, intracervical hyaluronidase was able to enhance the cervical tissue collagen dispersion.
本研究评估了宫颈内透明质酸酶对成功扭转子宫的水牛宫颈组织形态学的影响。这些动物要么接受常规的变形后治疗(n=10),要么在常规治疗之外,给予透明质酸酶(10,000 IU;在扭转后立即(n=10)在宫颈3、6、9、12点钟位置各给药2.5 ml,然后每隔6小时(0、6、12、18小时)重复给药,直至宫颈完全扩张或扭转后24小时。治疗前宫颈活检标本均于扭转后立即采集,治疗后标本均于扭转后24 h内宫颈完全扩张时采集。根据组织形态学,所有水牛的颈椎活检样品中胶原纤维的定量为胶原占总组织面积的平均百分比为21.0±5.7。治疗后,在宫颈完全扩张时,该值比对照组下降(9.1±2.2;p (gt;0.05)和透明质酸酶组(5.8±2.1;术中,0.05)。综上所述,成功扭转子宫的水牛的宫颈组织中胶原纤维的弥散表明其在宫颈扩张中的作用,因此,宫颈内透明质酸酶能够增强宫颈组织中的胶原弥散。
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