The present study was aimed to assess the type and prevalence of gross and microscopic lesions in ovaries of buffaloes obtained from abattoir. The prevalence of abnormalities of uterus were highest (17.02%; 181 samples) followed by ovaries (7.98%; 85 samples) among ten different types of anatomic abnormalities with overall prevalence of 29.83% (317/1062). Among the 85 ovarian lesions, the most observed lesions were sub active ovaries (34.11%), par ovarian cyst (20%), ovarobursal adhesion (20%), and cystic ovary (20%). To decipher ovarian hypofunction and other abnormalities, per-rectal examination and transrectal ultrasonography is used with variable degree of success to diagnose unilateral small sized pathological affections. In addition to visualize reproductive organs, ultrasonography also helpful to assess morphotype, localization, number, and size as well as shape of internal formations. The project sought to procure basic data for further investigation that would enable providing scientific support for methods to boost production through full utilization of the bubaline genetic potential.
{"title":"Macro and microscopic examination of ovarian pathologies in abattoir samples of buffalo of Agra region, Uttar Pradesh, India","authors":"Sushant Srivastava, Vijay Gautam, Pushkar Sharma, Saurabh Verma, Rajesh Kumar, Rabindra Kumar","doi":"10.56825/bufbu.2023.4243808","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4243808","url":null,"abstract":"The present study was aimed to assess the type and prevalence of gross and microscopic lesions in ovaries of buffaloes obtained from abattoir. The prevalence of abnormalities of uterus were highest (17.02%; 181 samples) followed by ovaries (7.98%; 85 samples) among ten different types of anatomic abnormalities with overall prevalence of 29.83% (317/1062). Among the 85 ovarian lesions, the most observed lesions were sub active ovaries (34.11%), par ovarian cyst (20%), ovarobursal adhesion (20%), and cystic ovary (20%). To decipher ovarian hypofunction and other abnormalities, per-rectal examination and transrectal ultrasonography is used with variable degree of success to diagnose unilateral small sized pathological affections. In addition to visualize reproductive organs, ultrasonography also helpful to assess morphotype, localization, number, and size as well as shape of internal formations. The project sought to procure basic data for further investigation that would enable providing scientific support for methods to boost production through full utilization of the bubaline genetic potential.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":"120 25","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139134116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to determine the presence of Chlamydia spp. in a dual-purpose zebu and water buffalo-mixed herd cohabitating in a ranch located in southern Mexico. The zootechnical purpose of this herd is milk and cheese production. A total of 52 vaginal exudate samples were obtained from clinically healthy water buffaloes, less than one month after parturition, and two vaginal exudate samples were taken from cows that had recently aborted. L929 cells were used for bacterial isolation. Two of these cultures were infected, confirmed by direct immunofluorescence. Total DNA was extracted for analysis with two types of real-time PCR, a Chlamydiaceae-specific real-time PCR and another species-specific real-time PCR for C. abortus, C. psittaci and C. pecorum. Two Chlamydia isolates were obtained, one from a water buffalo positive to C. abortus and C. pecorum, the other from a cow positive to C. abortus. This is the first report of the presence of C. abortus and C. pecorum in water buffaloes in Mexico. C. abortus was also detected in one cow living in the same herd as the water buffaloes.
{"title":"Identification of Chlamydia abortus and Chlamydia pecorum in water buffaloes (Bubalus bubalis) and cows cohabitating the same herd","authors":"Magdalena Limón–González, R. Hernández-Castro, Gabriela Palomares Reséndiz, Enrique Herrera López, Efrén Díaz Aparicio","doi":"10.56825/bufbu.2023.4243989","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4243989","url":null,"abstract":"The aim of this study was to determine the presence of Chlamydia spp. in a dual-purpose zebu and water buffalo-mixed herd cohabitating in a ranch located in southern Mexico. The zootechnical purpose of this herd is milk and cheese production. A total of 52 vaginal exudate samples were obtained from clinically healthy water buffaloes, less than one month after parturition, and two vaginal exudate samples were taken from cows that had recently aborted. L929 cells were used for bacterial isolation. Two of these cultures were infected, confirmed by direct immunofluorescence. Total DNA was extracted for analysis with two types of real-time PCR, a Chlamydiaceae-specific real-time PCR and another species-specific real-time PCR for C. abortus, C. psittaci and C. pecorum. Two Chlamydia isolates were obtained, one from a water buffalo positive to C. abortus and C. pecorum, the other from a cow positive to C. abortus. This is the first report of the presence of C. abortus and C. pecorum in water buffaloes in Mexico. C. abortus was also detected in one cow living in the same herd as the water buffaloes.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":"78 14","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139130537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-31DOI: 10.56825/bufbu.2023.4243847
Meenakshi Rawat, Mridula Sharma, Kamal Devlal, Dinesh Pandey, Harihar Prasad Gupta, M. Rana
The present study was conducted to observe effect of percoll density gradient centrifugation of buffalo bull semen on quantity of X sperms. Ejaculates were collected by artificial vagina method. Semen with mass motility >+3 and progressive motility >70 % were selected for experiment. X sperm Enrichment of semen was done by discontinuous percoll density gradient centrifugation and three groups were formed ie Group 1 (3 layer 70%, 50% and 30%) Group 2 (7 layer 70%, 60%, 50%, 40%, 30%, 20% and 10%) Group 3 (7 layer 80%, 70%, 60%, 50%, 40%, 30% and 20%). Centrifugation of semen of three groups and control (fresh semen without gradients) was done. After centrifugation, the supernatant part was removed and the pellet of each group was used for X sperm enrichment assessment by Raman spectroscopy. Results revealed that X sperm enrichment was higher in the pellets of Group 2 followed Group 3, Group 1 and Control as Raman peaks on DNA specific bands corresponds to more number of x sperm were higher respectively.
本研究旨在观察对水牛精液进行 percoll 密度梯度离心对 X 精子数量的影响。采用人工阴道法收集射精。实验选择了大量运动能力大于+3 和渐进运动能力大于 70% 的精液。精液中 X 精子的富集是通过不连续 percoll 密度梯度离心法进行的，共分为三组，即第一组（3 层 70%、50% 和 30%）、第二组（7 层 70%、60%、50%、40%、30%、20% 和 10%）、第三组（7 层 80%、70%、60%、50%、40%、30% 和 20%）。对三组精液和对照组（无梯度的新鲜精液）进行离心。离心后，去掉上清液部分，取各组的沉淀，用拉曼光谱评估 X 精子的富集情况。结果显示，第 2 组、第 3 组、第 1 组和对照组的颗粒中 X 精子富集度较高，因为 DNA 特定条带上的拉曼峰分别对应较多的 X 精子。
{"title":"Quantification of X sperm by raman spectroscopy in percoll density gradient centrifuged buffalo semen","authors":"Meenakshi Rawat, Mridula Sharma, Kamal Devlal, Dinesh Pandey, Harihar Prasad Gupta, M. Rana","doi":"10.56825/bufbu.2023.4243847","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4243847","url":null,"abstract":"The present study was conducted to observe effect of percoll density gradient centrifugation of buffalo bull semen on quantity of X sperms. Ejaculates were collected by artificial vagina method. Semen with mass motility >+3 and progressive motility >70 % were selected for experiment. X sperm Enrichment of semen was done by discontinuous percoll density gradient centrifugation and three groups were formed ie Group 1 (3 layer 70%, 50% and 30%) Group 2 (7 layer 70%, 60%, 50%, 40%, 30%, 20% and 10%) Group 3 (7 layer 80%, 70%, 60%, 50%, 40%, 30% and 20%). Centrifugation of semen of three groups and control (fresh semen without gradients) was done. After centrifugation, the supernatant part was removed and the pellet of each group was used for X sperm enrichment assessment by Raman spectroscopy. Results revealed that X sperm enrichment was higher in the pellets of Group 2 followed Group 3, Group 1 and Control as Raman peaks on DNA specific bands corresponds to more number of x sperm were higher respectively.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":"6 14","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139135855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-31DOI: 10.56825/bufbu.2023.4244454
Amit Kumar, Gyan Singh, J. Andonissamy, Pradeep Kumar, Arjun Venkateshappa, Renu Bala, N. Verma, C. Patil, Rakesh Kumar Sharma
This study was aimed to decipher the inter-relationship peripheral hormones [Insulin-like growth hormone (IGF-1), testosterone and growth hormone] with body weight, body condition score and scrotal circumfermnce across age-groups in male buffalo. Male buffalo (n=20) of different age groups viz. Group 1 (0 to 8 months), 2 (9 to 16 months), 3 (17 to 24 months) and 4 (25 to 32 months) were selected and Blood was collected along with body weight, body condition score and scrotal circumference. Significant difference (P<0.05) in the body weight, body condition core and scrotal circumference was observed between the groups. Peripheral IGF-1 level increased with age, highest in Group 4 (202.4±9.36 ng/ml). Similarly, testosterone was different between Group 1, 2 and 4, highest in Group 4 (1.73±0.02 ng/ml). Growth hormone, differed (P<0.05) between Group 1 (3.65±0.50 ng/ml), Group 3 (3.65±0.50 ng/ml) and Group 4 (8.56±1.96 ng/ml). Postive correlation (P<0.05) between various parameters (body weight, body condition score and scrotal circumference, testosterone and growth hormone) was observed. In conclusion, this study reports the age-related variations and inter-relationships of peripheral hormones with body weight, body condition score and scrotal circumference in male buffalo.
{"title":"Inter-relationship of peripheral hormones (IGF-1, testosterone and growth hormone) with reproductive traits in male buffalo","authors":"Amit Kumar, Gyan Singh, J. Andonissamy, Pradeep Kumar, Arjun Venkateshappa, Renu Bala, N. Verma, C. Patil, Rakesh Kumar Sharma","doi":"10.56825/bufbu.2023.4244454","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4244454","url":null,"abstract":"This study was aimed to decipher the inter-relationship peripheral hormones [Insulin-like growth hormone (IGF-1), testosterone and growth hormone] with body weight, body condition score and scrotal circumfermnce across age-groups in male buffalo. Male buffalo (n=20) of different age groups viz. Group 1 (0 to 8 months), 2 (9 to 16 months), 3 (17 to 24 months) and 4 (25 to 32 months) were selected and Blood was collected along with body weight, body condition score and scrotal circumference. Significant difference (P<0.05) in the body weight, body condition core and scrotal circumference was observed between the groups. Peripheral IGF-1 level increased with age, highest in Group 4 (202.4±9.36 ng/ml). Similarly, testosterone was different between Group 1, 2 and 4, highest in Group 4 (1.73±0.02 ng/ml). Growth hormone, differed (P<0.05) between Group 1 (3.65±0.50 ng/ml), Group 3 (3.65±0.50 ng/ml) and Group 4 (8.56±1.96 ng/ml). Postive correlation (P<0.05) between various parameters (body weight, body condition score and scrotal circumference, testosterone and growth hormone) was observed. In conclusion, this study reports the age-related variations and inter-relationships of peripheral hormones with body weight, body condition score and scrotal circumference in male buffalo.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":" 17","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139135546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-31DOI: 10.56825/bufbu.2023.4244266
G. Singh, Anil Gattani, Ajeet Kumar, Anil Kumar
The minor surgical intervention and safe restraining requires sedation and analgesia especially in furious animals like buffaloes which can be achieved by using proper combination of sedative and analgesic drugs. The combination of xylazine with opioids is an effective way to achieve sedation and analgesia in cattle and horses. Six apparently healthy buffaloes having minor wounds were included in the present study. The sedation was accomplished by fentanyl (5 µg per kg b.wt) plus xylazine (0.05 mg per kg b.wt) intravenously. Physiological, hematobiochemical and electrocardiographic parameters were observed at different time interval after the administration of drugs. Following the administration of medicine, there was a significant reduction in heart rate, systolic blood pressure, diastolic blood pressure, mean arterial blood pressure, hemoglobin, and packed cell volume. Respiration rate increased significantly at 30 minutes interval. PR interval and QT interval increased significantly at 20 minutes and 15 minutes respectively of the observation period, whereas a significant increase in the duration of ST segment was noticed from 15 minutes to 30 minutes. The present study revealed that the drug combination has no deleterious effects on cardiopulmonary, hematobiochemical and physiological parameters during the observation period.
{"title":"Cardiac and physico-chemical responses to xylazine and fentanyl analgesia in Bubalus bubalis","authors":"G. Singh, Anil Gattani, Ajeet Kumar, Anil Kumar","doi":"10.56825/bufbu.2023.4244266","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4244266","url":null,"abstract":"The minor surgical intervention and safe restraining requires sedation and analgesia especially in furious animals like buffaloes which can be achieved by using proper combination of sedative and analgesic drugs. The combination of xylazine with opioids is an effective way to achieve sedation and analgesia in cattle and horses. Six apparently healthy buffaloes having minor wounds were included in the present study. The sedation was accomplished by fentanyl (5 µg per kg b.wt) plus xylazine (0.05 mg per kg b.wt) intravenously. Physiological, hematobiochemical and electrocardiographic parameters were observed at different time interval after the administration of drugs. Following the administration of medicine, there was a significant reduction in heart rate, systolic blood pressure, diastolic blood pressure, mean arterial blood pressure, hemoglobin, and packed cell volume. Respiration rate increased significantly at 30 minutes interval. PR interval and QT interval increased significantly at 20 minutes and 15 minutes respectively of the observation period, whereas a significant increase in the duration of ST segment was noticed from 15 minutes to 30 minutes. The present study revealed that the drug combination has no deleterious effects on cardiopulmonary, hematobiochemical and physiological parameters during the observation period.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":"71 9","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139130498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The goal of the current study was to assess the efficacy of powder Harbominvit in non-infectious repeat breeder buffaloes with a selective test such as white side test, cervical pH, PMNs cell count, Fern patten and estimation of serum phosphorous. 24 buffaloes that tested negative for white side test was chosen for the current study out of a total of 51 buffaloes that underwent the test from Nanded and Parbhani districts of Marathwada region in Maharashtra under infertility camps organized by NDDB, Mother dairy, and Collage of animal and veterinary science Parbhani. MAFSU. In the Treatment group at day 0 before treatment repeat breeder buffaloes showed a Mean ± SE cervical pH value was 7.38±0.07 while the Control group showed a Mean ± SE cervical pH value was 7.59±0.08. The white side test was 100% negative for both groups. The mean ± SE value for PMN cell count for the Treatment group is 2.67±0.31 while the Control group was 2.08±0.22. The mean ± SE value for serum phosphorus value for the Treatment group before treatment is 3.81a±0.11 and after treatment is 4.937a±0.18 while the Control group shows before 3.68a±0.09 and after treatment 4.17a±0.20 and mean ± SE value for Fern pattern for Treatment group and Control group was 0.750±0.13 and 0.66±0.14 respectively. The conception rates in buffaloes of Group 1 with the treatment of powder Harbominvit and Group 2 with no treatment serve as control were 75% (09/12), and 41.66 % (05/12) respectively (Table 2). The overall conception rate in the present study was 58.33% (14/24) in non-infectious repeat-breeding buffaloes. As an herbal combination with chelated minerals and some vitamins has best conception rate in non-infectious repeat breeder buffaloes having the advantage of reducing the cost of treatment in repeat breeding as compared to other treatment protocols.
{"title":"Therapeutic management of non-infectious repeat breeder buffaloes by using phytomedicine","authors":"Vaibhav Vijay Choutmal, Manjusha Ganeshrao Patil, Nitin Manmohan Markandeya, Pankaj Bhanudas Hase","doi":"10.56825/bufbu.2023.4235405","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4235405","url":null,"abstract":"The goal of the current study was to assess the efficacy of powder Harbominvit in non-infectious repeat breeder buffaloes with a selective test such as white side test, cervical pH, PMNs cell count, Fern patten and estimation of serum phosphorous. 24 buffaloes that tested negative for white side test was chosen for the current study out of a total of 51 buffaloes that underwent the test from Nanded and Parbhani districts of Marathwada region in Maharashtra under infertility camps organized by NDDB, Mother dairy, and Collage of animal and veterinary science Parbhani. MAFSU. In the Treatment group at day 0 before treatment repeat breeder buffaloes showed a Mean ± SE cervical pH value was 7.38±0.07 while the Control group showed a Mean ± SE cervical pH value was 7.59±0.08. The white side test was 100% negative for both groups. The mean ± SE value for PMN cell count for the Treatment group is 2.67±0.31 while the Control group was 2.08±0.22. The mean ± SE value for serum phosphorus value for the Treatment group before treatment is 3.81a±0.11 and after treatment is 4.937a±0.18 while the Control group shows before 3.68a±0.09 and after treatment 4.17a±0.20 and mean ± SE value for Fern pattern for Treatment group and Control group was 0.750±0.13 and 0.66±0.14 respectively. The conception rates in buffaloes of Group 1 with the treatment of powder Harbominvit and Group 2 with no treatment serve as control were 75% (09/12), and 41.66 % (05/12) respectively (Table 2). The overall conception rate in the present study was 58.33% (14/24) in non-infectious repeat-breeding buffaloes. As an herbal combination with chelated minerals and some vitamins has best conception rate in non-infectious repeat breeder buffaloes having the advantage of reducing the cost of treatment in repeat breeding as compared to other treatment protocols.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136142383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-13DOI: 10.56825/bufbu.2023.4233581
Girish Patil Shivanagowda, Ramakrishna Chitimalla, Nagappa Karabasanavar, Arup Ratan Sen
Farm-to-fork traceability has emerged as benchmark for meat quality assurance in the International meat market. India is the largest exporter of buffalo (Bubalus bubalis) meat in the world; nevertheless, need for indigenous traceability system based quality assurance protocols to enable exports to the developed countries has been increasingly emphasized. To address this gap, a complete protocol for achieving traceability was conceptualized and a traceability database (www.livestocktraceindia.in) was developed to support the Indian buffalo meat sector. Traceability model and the database established was field tested. This article provides brief information of the traceability framework and the mode of its operation. The traceability database encompasses enrolment of animals, farms and abattoirs. Animal identification was achieved using ear tags with Internationally accepted identification numbers. Premises including farms and abattoirs were identified using unique pin code based system. Provision was given to end user to retrieve information and trace back the origin of meat using the database’s retrieval system. Database and traceability protocols developed can help promote livestock sector, meat traceability and meat export in India. The database can act as model for establishment of traceability system in other countries producing and exporting the buffalo meat.
{"title":"A database for buffalo meat traceability in India","authors":"Girish Patil Shivanagowda, Ramakrishna Chitimalla, Nagappa Karabasanavar, Arup Ratan Sen","doi":"10.56825/bufbu.2023.4233581","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4233581","url":null,"abstract":"Farm-to-fork traceability has emerged as benchmark for meat quality assurance in the International meat market. India is the largest exporter of buffalo (Bubalus bubalis) meat in the world; nevertheless, need for indigenous traceability system based quality assurance protocols to enable exports to the developed countries has been increasingly emphasized. To address this gap, a complete protocol for achieving traceability was conceptualized and a traceability database (www.livestocktraceindia.in) was developed to support the Indian buffalo meat sector. Traceability model and the database established was field tested. This article provides brief information of the traceability framework and the mode of its operation. The traceability database encompasses enrolment of animals, farms and abattoirs. Animal identification was achieved using ear tags with Internationally accepted identification numbers. Premises including farms and abattoirs were identified using unique pin code based system. Provision was given to end user to retrieve information and trace back the origin of meat using the database’s retrieval system. Database and traceability protocols developed can help promote livestock sector, meat traceability and meat export in India. The database can act as model for establishment of traceability system in other countries producing and exporting the buffalo meat.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135919689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-06DOI: 10.56825/bufbu.2023.4232549
Chandrvadan Laxman Badgujar, Satyawan Madhukar Agivale, Shaikh Azmat Shaikh Maulana, Abdul Khyum Noor Mysore
Epidural anaesthesia in buffaloes is done at sacro coccygeal or at first inter-coccygeal space. A new technique of injecting epidural anaesthesia was compared with traditional technique at first inter coccygeal space. Two group were done with Group 1 consisting of 10 cases and Group 2 with 6 cases were with affections of Tail gangrene, prolapse of vagina, caesarean section, vaginal tear and tail wound studied at Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Udgir. Group 1 was anesthetized by traditional method by inserting needle at 45 degree angle at first inter coccygeal space. Group 2 was anaesthetized by catheterization where in first as traditional technique 16 G spinal needle passed into epidural space then catheter was inserted and directed inside 1 to 1.5 inch inside and placed by confirming site. Study showed catheterization useful when incremental dose is necessary. It required care and experience to maintain catheter in position. Injury or trauma was minimum compared to traditional technique.
{"title":"Epidural anaesthesia with traditional technique versus epidural catheterization technique in buffaloes","authors":"Chandrvadan Laxman Badgujar, Satyawan Madhukar Agivale, Shaikh Azmat Shaikh Maulana, Abdul Khyum Noor Mysore","doi":"10.56825/bufbu.2023.4232549","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4232549","url":null,"abstract":"Epidural anaesthesia in buffaloes is done at sacro coccygeal or at first inter-coccygeal space. A new technique of injecting epidural anaesthesia was compared with traditional technique at first inter coccygeal space. Two group were done with Group 1 consisting of 10 cases and Group 2 with 6 cases were with affections of Tail gangrene, prolapse of vagina, caesarean section, vaginal tear and tail wound studied at Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Udgir. Group 1 was anesthetized by traditional method by inserting needle at 45 degree angle at first inter coccygeal space. Group 2 was anaesthetized by catheterization where in first as traditional technique 16 G spinal needle passed into epidural space then catheter was inserted and directed inside 1 to 1.5 inch inside and placed by confirming site. Study showed catheterization useful when incremental dose is necessary. It required care and experience to maintain catheter in position. Injury or trauma was minimum compared to traditional technique.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134945263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plasma melatonin vis-a-vis anestrus or estrus status of buffaloes during summer or winter season (n=50 in each group) suggested its impact on reproductive axis as plasma melatonin activity tended to be higher (P>0.05) in buffaloes exhibiting estrus compared to their anestrous counterparts irrespective of season. The differential reproductive status of these buffaloes was confirmed by history as well as differences (P<0.05) in diameter of largest follicle and luteal profile. Further, for evaluating the impact of melatonin treatment during summer season on reproductive axis, 132 anestrous buffaloes were subcutaneously inserted 2x4 mm absorbable slow-release melatonin implants (18 mg/50 kg b wt) at the base of left ear and 60 buffaloes were used as control. In these buffaloes, ovarian ultrasonography and jugular vein blood sampling was carried out at 7-day interval till day 35 post-treatment or till ovulation, whichever was earlier. Control and implanted buffaloes were subjected to artificial insemination (AI) at overt or induced estrus followed by pregnancy diagnosis at day 90 post-AI. In treatment group, an increase (P<0.05) in plasma melatonin was recorded in all the buffaloes, exhibiting differential fertility status, during post-treatment study period compared to their pre-treatment and control group values. However, within treatment group, there was no difference (P>0.05) in plasma melatonin between ovulatory or non-ovulatory as well as between pregnant or non-pregnant counterparts. Moreover, plasma melatonin within control buffaloes remained similar (P>0.05) throughout the study period irrespective of differential exhibition of fertility. It can be concluded that factors other than circulating melatonin are also involved in the display of differential fertility in terms of initiation of ovarian cyclicity/ovulation or ability to conceive in summer anestrous buffaloes.
血浆褪黑素与夏季或冬季水牛的发情或发情状态的关系(每组n=50)表明其对生殖轴的影响，因为无论季节如何，处于发情状态的水牛血浆褪黑素活性往往高于处于无情状态的水牛(P>0.05)。历史以及最大卵泡直径和黄体轮廓的差异(P<0.05)证实了这些水牛的不同生殖状态。此外，为了评估夏季褪黑素治疗对生殖轴的影响，我们在132头发情水牛的左耳基部皮下植入2x4 mm可吸收的缓释褪黑素植入物(18 mg/50 kg b wt)，并以60头水牛作为对照。每隔7天进行卵巢超声检查和颈静脉采血，直至治疗后第35天或排卵前(以较早者为准)。对照和植入水牛在明显或诱导发情时进行人工授精，并在人工授精后第90天进行妊娠诊断。在治疗组，与治疗前和对照组相比，在治疗后的研究期间，所有水牛血浆褪黑素均增加(P<0.05)，表现出不同的生育状态。而在治疗组内，排卵期与非排卵期、孕妇与非孕妇血浆褪黑素水平差异无统计学意义(P>0.05)。此外，在整个研究期间，对照水牛的血浆褪黑素保持相似(P>0.05)，而不考虑生育表现的差异。由此可以得出结论，除循环褪黑素外，其他因素也参与了在卵巢周期/排卵启动或夏季无情水牛受孕能力方面表现出的差异生育能力。
{"title":"Assessment of plasma melatonin profile in summer anestrous buffaloes exhibiting differential fertility following melatonin implants treatment","authors":"Sarvpreet Singh Ghuman, Mrignak Honparkhe, Bilawal Singh","doi":"10.56825/bufbu.2023.4233571","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4233571","url":null,"abstract":"Plasma melatonin vis-a-vis anestrus or estrus status of buffaloes during summer or winter season (n=50 in each group) suggested its impact on reproductive axis as plasma melatonin activity tended to be higher (P>0.05) in buffaloes exhibiting estrus compared to their anestrous counterparts irrespective of season. The differential reproductive status of these buffaloes was confirmed by history as well as differences (P<0.05) in diameter of largest follicle and luteal profile. Further, for evaluating the impact of melatonin treatment during summer season on reproductive axis, 132 anestrous buffaloes were subcutaneously inserted 2x4 mm absorbable slow-release melatonin implants (18 mg/50 kg b wt) at the base of left ear and 60 buffaloes were used as control. In these buffaloes, ovarian ultrasonography and jugular vein blood sampling was carried out at 7-day interval till day 35 post-treatment or till ovulation, whichever was earlier. Control and implanted buffaloes were subjected to artificial insemination (AI) at overt or induced estrus followed by pregnancy diagnosis at day 90 post-AI. In treatment group, an increase (P<0.05) in plasma melatonin was recorded in all the buffaloes, exhibiting differential fertility status, during post-treatment study period compared to their pre-treatment and control group values. However, within treatment group, there was no difference (P>0.05) in plasma melatonin between ovulatory or non-ovulatory as well as between pregnant or non-pregnant counterparts. Moreover, plasma melatonin within control buffaloes remained similar (P>0.05) throughout the study period irrespective of differential exhibition of fertility. It can be concluded that factors other than circulating melatonin are also involved in the display of differential fertility in terms of initiation of ovarian cyclicity/ovulation or ability to conceive in summer anestrous buffaloes.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135646793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-04DOI: 10.56825/bufbu.2023.4235464
Rajesh Ranjan, Opinder Singh, Devendra Pathak
The present study was conducted on placentomes of 31 pregnant buffaloes ranging from 38 to 243 days of gestation to explore the microscopic details of giant cells and their behavior in buffalo placentomes. Various histological and histochemical stains used in the study revealed its structural and chemical details. The study revealed that giant cells played a major role in transplacental transfer of nutrients and other metabolites required by the fetal and maternal tissues. High polysaccharide content and intense enzymatic reaction indicated high metabolic activity of the giant cells in the placenta. The migratory nature of giant cells observed in the present study revealed its role in transfer of metabolites. The cytoplasmic processes observed in the study indicated its fusion with the cryptal epithelium as a medium of transfer of metabolites and formation of multinucleated giant cells. Strong acid phosphates activity can be correlated with its erythrophagocytic nature as a medium of transfer of iron molecules to the developing fetus.
{"title":"Microscopic exploration into the behavior of giant cells in placentomes of buffalo (Bubalus bubalis)","authors":"Rajesh Ranjan, Opinder Singh, Devendra Pathak","doi":"10.56825/bufbu.2023.4235464","DOIUrl":"https://doi.org/10.56825/bufbu.2023.4235464","url":null,"abstract":"The present study was conducted on placentomes of 31 pregnant buffaloes ranging from 38 to 243 days of gestation to explore the microscopic details of giant cells and their behavior in buffalo placentomes. Various histological and histochemical stains used in the study revealed its structural and chemical details. The study revealed that giant cells played a major role in transplacental transfer of nutrients and other metabolites required by the fetal and maternal tissues. High polysaccharide content and intense enzymatic reaction indicated high metabolic activity of the giant cells in the placenta. The migratory nature of giant cells observed in the present study revealed its role in transfer of metabolites. The cytoplasmic processes observed in the study indicated its fusion with the cryptal epithelium as a medium of transfer of metabolites and formation of multinucleated giant cells. Strong acid phosphates activity can be correlated with its erythrophagocytic nature as a medium of transfer of iron molecules to the developing fetus.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135646794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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