Charles Amele Ndjoumoui, Mathieu Tchideme, Edwin Dinayen Somnjom
The ever-increasing urbanisation and deforestation in the metropolises of the Eastern region has led to the loss of a great diversity of fruit and even forest species. In view of this difficulty, a study was carried out on diversity of fruit species and production contraints in home gardens in the metropolises of Eastern region of Cameroon. The methodological approach involved snowball sampling through the administration of a semi-structured questionnaire to 189 home gardens in the districts of Mandjou, Bertoua II and Batouri. The results showed a diversity of 15 fruit species corresponding to an abundance of 4126 individuals. The mandarin tree is the most cultivated species (1153 trees). Young trees (<10) are in the majority in Bertoua (55,3%) and Mandjou (60,5%). Seeds are mainly sourced from local markets (73%) and trees maintenance is almost non-existent (86,4%). The most important production contraint is low production (41,6%). This study highlighted the diversity of fruit species and production contraints in home gardens in the East Cameroon region. Key words : Diversity, fruit species, production contraints, home gardens.
{"title":"Diversité des espèces fruitières et contraintes de production des jardins de case des métropoles de la Région de l’Est Cameroun","authors":"Charles Amele Ndjoumoui, Mathieu Tchideme, Edwin Dinayen Somnjom","doi":"10.4314/cajeb.v15i1.4","DOIUrl":"https://doi.org/10.4314/cajeb.v15i1.4","url":null,"abstract":"The ever-increasing urbanisation and deforestation in the metropolises of the Eastern region has led to the loss of a great diversity of fruit and even forest species. In view of this difficulty, a study was carried out on diversity of fruit species and production contraints in home gardens in the metropolises of Eastern region of Cameroon. The methodological approach involved snowball sampling through the administration of a semi-structured questionnaire to 189 home gardens in the districts of Mandjou, Bertoua II and Batouri. The results showed a diversity of 15 fruit species corresponding to an abundance of 4126 individuals. The mandarin tree is the most cultivated species (1153 trees). Young trees (<10) are in the majority in Bertoua (55,3%) and Mandjou (60,5%). Seeds are mainly sourced from local markets (73%) and trees maintenance is almost non-existent (86,4%). The most important production contraint is low production (41,6%). This study highlighted the diversity of fruit species and production contraints in home gardens in the East Cameroon region. \u0000Key words : Diversity, fruit species, production contraints, home gardens.","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78095731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. B. Bilong Bilong, M. Oyono, S. Fosso, L. Lehman
During multiparasitism, the interspecific association among parasite species occur at the single host, population and community levels. Their detection and understanding are crucial to prevent and manage infectious diseases. In order to find out potential interspecific interactions among parasite species at a host population level, a cross-sectional study was carried out from September 2017 to July 2018 on schoolchildren aged from 4 to 15 years old; primary schools were randomly selected in the Nyong-et-Mfoumou Division. Stool samples and blood smears were analysed to detect parasitic forms of protozoa and helminths. Parasite interspecific associations were explored by ecological indices of association: Dice (D), Forbes (F) and tetrachoric coefficient (φ). The parasitological analysis revealed the presence of 13 parasite species belonging to 11 families, 9 orders, 7 classes and 5 phyla. A cooperation or positive association was found between E. coli and E. histolytica/dispar, A. lumbricoides and T. trichiura, E. coli and P. falciparum, E. histolytica/dispar and P. falciparum, E. coli and A. lumbricoides, and E. coli and T. trichiura. They co-occurred together more frequently than expected by chance. The conflict or negative association was noticed between G. intestinalis and both A. lumbricoides and T. trichiura, and between A. lumbricoides and P. falciparum. The independence was found between G. intestinalis and both E. histolytica/dispar and E. coli, and between M. perstans and En. nana, G. intestinalis, E. coli and E. histolytica/dispar. Further studies are needed to identify the real interaction mechanisms between parasite species and to evaluate the consequences of multiparasitism for both parasite species and the host.
{"title":"Ecological characterization of interspecific relationships between human parasites: conflict, cooperation or independence?","authors":"C. B. Bilong Bilong, M. Oyono, S. Fosso, L. Lehman","doi":"10.4314/cajeb.v15i1.2","DOIUrl":"https://doi.org/10.4314/cajeb.v15i1.2","url":null,"abstract":" During multiparasitism, the interspecific association among parasite species occur at the single host, population and community levels. Their detection and understanding are crucial to prevent and manage infectious diseases. In order to find out potential interspecific interactions among parasite species at a host population level, a cross-sectional study was carried out from September 2017 to July 2018 on schoolchildren aged from 4 to 15 years old; primary schools were randomly selected in the Nyong-et-Mfoumou Division. Stool samples and blood smears were analysed to detect parasitic forms of protozoa and helminths. Parasite interspecific associations were explored by ecological indices of association: Dice (D), Forbes (F) and tetrachoric coefficient (φ). The parasitological analysis revealed the presence of 13 parasite species belonging to 11 families, 9 orders, 7 classes and 5 phyla. A cooperation or positive association was found between E. coli and E. histolytica/dispar, A. lumbricoides and T. trichiura, E. coli and P. falciparum, E. histolytica/dispar and P. falciparum, E. coli and A. lumbricoides, and E. coli and T. trichiura. They co-occurred together more frequently than expected by chance. The conflict or negative association was noticed between G. intestinalis and both A. lumbricoides and T. trichiura, and between A. lumbricoides and P. falciparum. The independence was found between G. intestinalis and both E. histolytica/dispar and E. coli, and between M. perstans and En. nana, G. intestinalis, E. coli and E. histolytica/dispar. Further studies are needed to identify the real interaction mechanisms between parasite species and to evaluate the consequences of multiparasitism for both parasite species and the host. \u0000 ","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86230959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jonathan Moucharou Ngnignigniwou, Junior Baudoin Wouokoue Taffo, V. Nguetsop
This study was conducted to characterize the floristic, structural and ecological diversity of the Mawouon Forest in the Noun division (Western Highlands Cameroon). In total, 14 plots of 30 m x 100 m (3000 m²) were laid out. Within each, all individuals with a diameter ≥ 10 cm at 1.30 m in height were measured. A total of 2324 individuals belonging to 102 species, 78 genera and 35 families have been identified. The richest family was the Fabaceae (15 species) followed by Euphorbiaceae (12 species) and Moraceae (10 species). The most abundant family was the Rubiaceae (26.97%) followed by Euphorbiaceae (17.16%) and Cecropiaceae (12.65%). The Shannon diversity index was 4.51 bits and the Pielou evenness index was 0.67. Alchornea laxiflora with 89.21% of Important Value index was most importance species followed by Myrianthus arboreus (89.03%) and Polyscias fulva (79.12%). The diameter structure shows a predominance of young individuals. The abundance of megaphanerophytes and mesophanerophytes reflects the adaptive plant strategies that correspond to the competitive strategy. The relative abundance of Guinean-Congolese domain species translates a great maturity of this forest that seems little or not disturbed by human activity.
{"title":"Woody species diversity and ecological characteristics of the Mawouon forest, in the Western Highlands of Cameroon","authors":"Jonathan Moucharou Ngnignigniwou, Junior Baudoin Wouokoue Taffo, V. Nguetsop","doi":"10.4314/cajeb.v15i1.5","DOIUrl":"https://doi.org/10.4314/cajeb.v15i1.5","url":null,"abstract":"This study was conducted to characterize the floristic, structural and ecological diversity of the Mawouon Forest in the Noun division (Western Highlands Cameroon). In total, 14 plots of 30 m x 100 m (3000 m²) were laid out. Within each, all individuals with a diameter ≥ 10 cm at 1.30 m in height were measured. A total of 2324 individuals belonging to 102 species, 78 genera and 35 families have been identified. The richest family was the Fabaceae (15 species) followed by Euphorbiaceae (12 species) and Moraceae (10 species). The most abundant family was the Rubiaceae (26.97%) followed by Euphorbiaceae (17.16%) and Cecropiaceae (12.65%). The Shannon diversity index was 4.51 bits and the Pielou evenness index was 0.67. Alchornea laxiflora with 89.21% of Important Value index was most importance species followed by Myrianthus arboreus (89.03%) and Polyscias fulva (79.12%). The diameter structure shows a predominance of young individuals. The abundance of megaphanerophytes and mesophanerophytes reflects the adaptive plant strategies that correspond to the competitive strategy. The relative abundance of Guinean-Congolese domain species translates a great maturity of this forest that seems little or not disturbed by human activity.","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"127 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77609828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The incidence of healthy pregnant women carrying CTX-M-type extended-spectrum beta-lactamase (ESBL)-producing E. coli and their transmission to neonates is increasing worldwide. ESBL-E coli and especially the carriage of CTX-M-type causes early or late onset of neonatal sepsis, resulting in increased morbidity and mortality rates. Although maternal carriage and maternal-neonatal transmissions of ESBL-E have been reported in several countries, the prevalence of CTX-M-type ESBL-producing E. coli in pregnant women and its transmission to newborns at birth in Cameroon has not been reported yet. We describe here the carriage of CTX-M-type ESBL-producing E. coli pregnant women in neonatal ward of the Yaoundé gyneco-obstetric and pediatric hospital and their transmission to newborns. Among the 102 pregnant women and their newborns present in the ward, 88 (86.3%) and 75 (73.5%) E. coli strains were detected in rectal colonization, respectively. Antibiotic susceptibility testing of E. coli isolated from the mothers indicated a higher resistance rate to antibiotics of the β-lactams and sulfamide families, while the resistances to other antibiotic families (aminosides, quinolones and fluoroquinolones) were low. Comparatively, only cefotaxime (100%) showed a higher resistance rate to E. coli isolated from newborns. This may suggest a different source of contamination between mothers and newborns. Moreover, the rate of carriage of CTX-M-type ESBL-producing E. coli in pregnant mother and their newborns were 30.7 % and 14.7 %, respectively. This suggests that newborns had other colonization sources than the mothers. Indeed, multiple regression analysis indicated that newborns were exposed to CTX-M-type ESBL-producing E. coli from mothers and that from the hospital environment (eg. caregivers). Overall, the current investigation may provide insight on establishing an efficient therapeutic strategy against materno-neonatal and nosocomial transmission of CTX-M-type ESBL-producing E. coli.
{"title":"Antibiotic resistance pattern of extended-spectrum-beta lactamases-producing Escherichia coli isolated from pregnant women and their new born","authors":"Carol Suzie Titsamp Lacmago, Simon Ngamli Fewou","doi":"10.4314/cajeb.v15i1.1","DOIUrl":"https://doi.org/10.4314/cajeb.v15i1.1","url":null,"abstract":"The incidence of healthy pregnant women carrying CTX-M-type extended-spectrum beta-lactamase (ESBL)-producing E. coli and their transmission to neonates is increasing worldwide. ESBL-E coli and especially the carriage of CTX-M-type causes early or late onset of neonatal sepsis, resulting in increased morbidity and mortality rates. Although maternal carriage and maternal-neonatal transmissions of ESBL-E have been reported in several countries, the prevalence of CTX-M-type ESBL-producing E. coli in pregnant women and its transmission to newborns at birth in Cameroon has not been reported yet. We describe here the carriage of CTX-M-type ESBL-producing E. coli pregnant women in neonatal ward of the Yaoundé gyneco-obstetric and pediatric hospital and their transmission to newborns. Among the 102 pregnant women and their newborns present in the ward, 88 (86.3%) and 75 (73.5%) E. coli strains were detected in rectal colonization, respectively. Antibiotic susceptibility testing of E. coli isolated from the mothers indicated a higher resistance rate to antibiotics of the β-lactams and sulfamide families, while the resistances to other antibiotic families (aminosides, quinolones and fluoroquinolones) were low. Comparatively, only cefotaxime (100%) showed a higher resistance rate to E. coli isolated from newborns. This may suggest a different source of contamination between mothers and newborns. Moreover, the rate of carriage of CTX-M-type ESBL-producing E. coli in pregnant mother and their newborns were 30.7 % and 14.7 %, respectively. This suggests that newborns had other colonization sources than the mothers. Indeed, multiple regression analysis indicated that newborns were exposed to CTX-M-type ESBL-producing E. coli from mothers and that from the hospital environment (eg. caregivers). Overall, the current investigation may provide insight on establishing an efficient therapeutic strategy against materno-neonatal and nosocomial transmission of CTX-M-type ESBL-producing E. coli.","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81574933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hervé Sime Djomo, C. Suh, A. G. Mbong, Dély Carlos Deloko Temfack, H. Mafouasson, Michel d’Aquin Mbotchak Leumassi, Martial Stéphane Kamtchoum, T. Fonkou
Viruses are a limiting factor in maize production worldwide. Maize streak virus (MSV) significantly reduces maize yield in Africa and causes production losses ranging from 30 to 100%. In Cameroon, the incidence of maize streak disease ranges from 10 to 60% depending on the agro-ecological zone; and this is due to the susceptibility of the varieties used. A natural screening of 12 maize varieties for resistance to MSV was conducted at Institute of Agricultural Research and Development (IRAD) at Foumbot in 2019 and 2020, during the dry and rainy seasons. Prior to the trial, vector abundance was determined in the different localities of the Western Highlands of Cameroon. The incidence and severity of maize streak disease (MSD) was assessed at symptom onset for all varieties. The presence of MSV in leaf samples was confirmed by the Triple Antibody Sandwich Enzyme-Linked Immunosorbent Assay (TAS-ELISA). The results showed that leafhoppers were more abundant in the Foumbot locality. MSV infections are higher in the dry season than in the rainy season. The maize varieties KASSAÏ, ACR06, ATP and MADJSYN VAR2 are less susceptible to MSV. These varieties could be used in the control of maize streak disease to limit yield losses.
{"title":"Response of some maize (Zea mays L.) varieties to natural infection of Maize streak virus in the Western Highlands of Cameroon","authors":"Hervé Sime Djomo, C. Suh, A. G. Mbong, Dély Carlos Deloko Temfack, H. Mafouasson, Michel d’Aquin Mbotchak Leumassi, Martial Stéphane Kamtchoum, T. Fonkou","doi":"10.4314/cajeb.v15i1.3","DOIUrl":"https://doi.org/10.4314/cajeb.v15i1.3","url":null,"abstract":"Viruses are a limiting factor in maize production worldwide. Maize streak virus (MSV) significantly reduces maize yield in Africa and causes production losses ranging from 30 to 100%. In Cameroon, the incidence of maize streak disease ranges from 10 to 60% depending on the agro-ecological zone; and this is due to the susceptibility of the varieties used. A natural screening of 12 maize varieties for resistance to MSV was conducted at Institute of Agricultural Research and Development (IRAD) at Foumbot in 2019 and 2020, during the dry and rainy seasons. Prior to the trial, vector abundance was determined in the different localities of the Western Highlands of Cameroon. The incidence and severity of maize streak disease (MSD) was assessed at symptom onset for all varieties. The presence of MSV in leaf samples was confirmed by the Triple Antibody Sandwich Enzyme-Linked Immunosorbent Assay (TAS-ELISA). The results showed that leafhoppers were more abundant in the Foumbot locality. MSV infections are higher in the dry season than in the rainy season. The maize varieties KASSAÏ, ACR06, ATP and MADJSYN VAR2 are less susceptible to MSV. These varieties could be used in the control of maize streak disease to limit yield losses.","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76568204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kinjouo Ghislain Kenguem, V. Nguetsop, Solefack Marie Caroline Momo
Les forêts d’Afrique centrale contiennent plusieurs produits d’origine animale et végétale. Les produits d’origine végétale peuvent se distinguer en produits forestiers ligneux et non ligneux. Le présent travail de recherche porte sur l’importance et la gestion durable de quelques Produits Forestiers Non Ligneux (PFNL), notamment l’écorce de trois arbres (Garcinia lucida, Scorodophloeus zenkeri, Alstonia boonei) dans la localité de Ngovayang I. À l’aide d’un questionnaire semi-structuré, les caractéristiques du secteur des PFNL, les stratégies de gestion, ainsi que l’impact de la valorisation des PFNL ont été déterminés. Il ressort des enquêtes que les écorces de ces trois espèces sont connues et utilisées à des fins diverses telles que la consommation, l’automédication et la vente. L’initiation à l’utilisation de l’écorce de ces espèces est liée au sexe et est également fonction de l’âge des enquêtés. Leurs écorces sont approvisionnées sur le marché et vendues en majorité par les hommes. G. lucida et S. zenkeri sont utilisées à des fins nutritionnelles, médicinales et lucratives, tandis qu’Alstonia boonei est utilisée exclusivement à des fins médicinales. Pour des personnes plus jeunes, d’une tranche d’âge comprise entre 15 et 35 ans, l’exploitation de l’écorce de ces trois espèces n’a aucun impact néfaste sur la disponibilité des ressources forestières. S’agissant des personnes âgées de 35 à 65 ans, l’exploitation non contrôlée et l’écorçage anarchique de G. lucida et de S. zenkeri peuvent entrainer leur pénurie et même leur disparition dans cette région. Toutefois, la récolte échelonnée de l’écorce de G. lucida, S. zenkeri et A. boonei est utilisée comme stratégie de gestion de l’écorce de ces arbres dans cette localité. Central African forests are teemed with several goods of animal and plant origin. Goods of plant origin are forest products and non-timber forest products. This research work focused on the identification and importance of some non-timber forest products (NTFPs), especially the bark of tree species (Garcinia lucida, Scorodophloeus zenkeri and Alstonia boonei) in the locality of Ngovayang I. Using a semi-structured questionnaire, the characteristics of the NTFP sector, management strategies, and the impact of NTFP exploitation were determined in Ngovayang I. Surveys show that the bark of these three species is well known and used for various purposes such as consumption, self-medication and sale. The initiation of the use of the bark of these species is linked to sex and also depends on the age of the respondents. Their bark is supplied on the market and sold mostly by men. G. lucida and S. zenkeri are used for food, medicinal and profit-making purposes, while A. boonei is used exclusively for medicinal purposes. For younger people, aged between 15 and 35 years old, the exploitation of the bark of these three species has no negative impact on forest resources. For old people (35 to 65 years), the uncontrolled exploitation a
中非的森林含有许多动植物产品。植物源产品可分为木质和非木质森林产品。本研究工作涉及可持续森林管理的重要性和一些非木材林产品(软木树皮等)、三棵假丝酵母(lucida Scorodophloeus zenkeri、Alstonia boonei)镇的农人组织一、问卷、半正规部门的特点是从森林管理战略、以及森林中回收的影响已经确定。调查显示，这三种植物的树皮都是已知的，并被用于各种用途，如消费、自我治疗和销售。使用这些物种树皮的开始与性别有关，也与受访者的年龄有关。它们的树皮在市场上供应，主要由男性出售。G. lucida和S. zenkeri用于营养、医药和盈利目的，而alstonia boonei仅用于医药目的。对于年龄在15至35岁之间的年轻人来说，砍伐这三种树种的树皮对森林资源的可获得性没有不利影响。对于35 - 65岁的人来说，G. lucida和S. zenkeri的不受控制的开发和不受控制的剥皮可能导致他们在该地区短缺甚至消失。然而，在该地区，分阶段采集G. lucida、S. zenkeri和A. boonei的树皮被用作这些树的树皮管理策略。= =地理= =根据美国人口普查局的数据，该县的总面积为，其中土地和(1.)水。植物来源的货物是森林产品和非木材森林产品。鉴定and work This research研究on the怎么解释non-timber forest of products (NTFPs)的重要性,特别是皮of the tree,假丝酵母品种(lucida Scorodophloeus zenkeri and Alstonia boonei) in the i .利用locality of农人组织了semi-structured问卷、圣母NTFP sector management strategies)、the特征Ngovayang i确定了NTFP开发的影响。调查显示，这三个物种的树皮是众所周知的，并被用于各种用途，如消费、自我用药和销售。入门of The use of The树皮of these品种is挂钩to sex and also取决于第on The age of The过渡。他们的bark是在市场上供应的，大部分是男人。= =地理= =根据美国人口普查，这个县的面积为，其中土地面积为，其中土地面积为。对于15 - 35岁的年轻人来说，这三个物种的树皮的开发对森林资源没有负面影响。对于老年人(35 - 65岁)来说，G. lucida和S. zenkeri的不受控制的开发和砍伐可能导致该地区这两种树种的减少甚至灭绝。休息,计划harvest of the树皮、假丝酵母of lucida Scorodophloeus zenkeri and Alstonia boonei is used as strategy of management of the树皮trees of these in this locality)。
{"title":"Utilisation ethnobotanique de Garcinia lucida, Scorodophloeus zenkeri et Alstonia boonei dans la localité de Ngovayang I (Sud Cameroun)","authors":"Kinjouo Ghislain Kenguem, V. Nguetsop, Solefack Marie Caroline Momo","doi":"10.4314/cajeb.v14i2.4","DOIUrl":"https://doi.org/10.4314/cajeb.v14i2.4","url":null,"abstract":"Les forêts d’Afrique centrale contiennent plusieurs produits d’origine animale et végétale. Les produits d’origine végétale peuvent se distinguer en produits forestiers ligneux et non ligneux. Le présent travail de recherche porte sur l’importance et la gestion durable de quelques Produits Forestiers Non Ligneux (PFNL), notamment l’écorce de trois arbres (Garcinia lucida, Scorodophloeus zenkeri, Alstonia boonei) dans la localité de Ngovayang I. À l’aide d’un questionnaire semi-structuré, les caractéristiques du secteur des PFNL, les stratégies de gestion, ainsi que l’impact de la valorisation des PFNL ont été déterminés. Il ressort des enquêtes que les écorces de ces trois espèces sont connues et utilisées à des fins diverses telles que la consommation, l’automédication et la vente. L’initiation à l’utilisation de l’écorce de ces espèces est liée au sexe et est également fonction de l’âge des enquêtés. Leurs écorces sont approvisionnées sur le marché et vendues en majorité par les hommes. G. lucida et S. zenkeri sont utilisées à des fins nutritionnelles, médicinales et lucratives, tandis qu’Alstonia boonei est utilisée exclusivement à des fins médicinales. Pour des personnes plus jeunes, d’une tranche d’âge comprise entre 15 et 35 ans, l’exploitation de l’écorce de ces trois espèces n’a aucun impact néfaste sur la disponibilité des ressources forestières. S’agissant des personnes âgées de 35 à 65 ans, l’exploitation non contrôlée et l’écorçage anarchique de G. lucida et de S. zenkeri peuvent entrainer leur pénurie et même leur disparition dans cette région. Toutefois, la récolte échelonnée de l’écorce de G. lucida, S. zenkeri et A. boonei est utilisée comme stratégie de gestion de l’écorce de ces arbres dans cette localité. \u0000 \u0000Central African forests are teemed with several goods of animal and plant origin. Goods of plant origin are forest products and non-timber forest products. This research work focused on the identification and importance of some non-timber forest products (NTFPs), especially the bark of tree species (Garcinia lucida, Scorodophloeus zenkeri and Alstonia boonei) in the locality of Ngovayang I. Using a semi-structured questionnaire, the characteristics of the NTFP sector, management strategies, and the impact of NTFP exploitation were determined in Ngovayang I. Surveys show that the bark of these three species is well known and used for various purposes such as consumption, self-medication and sale. The initiation of the use of the bark of these species is linked to sex and also depends on the age of the respondents. Their bark is supplied on the market and sold mostly by men. G. lucida and S. zenkeri are used for food, medicinal and profit-making purposes, while A. boonei is used exclusively for medicinal purposes. For younger people, aged between 15 and 35 years old, the exploitation of the bark of these three species has no negative impact on forest resources. For old people (35 to 65 years), the uncontrolled exploitation a","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74310283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mbasso Joseph Eric Tameko, N. R. Nono, M. Frese, J. R. Chouna, N. Lenta, Pépin Nkeng-Efouet-Alango, A. L. Tapondjou, N. Sewald
Phytochemical investigation of the stem bark extract of Psychotria djumaensis led to the isolation of ten known pentacyclic triterpenoids among which one lupane-, three oleanane-, and six ursane-types. The structures of the isolated compounds were established by means of spectroscopic methods and by comparison with previously reported data. The isolated compounds were tested against five strains of bacteria, and the cytotoxicity against the human cervix carcinoma KB-3-1 cell was evaluated. The isolates exhibited weak antibacterial activity against the five strains. The cytotoxic activity exhibited by 3β,19α-dihydroxyurs-12-en-28-oic acid (5) (IC50 5.9 µM) was about three times more significant than that of the reference [(+) griseofulvin, IC50 17-21 µM].
{"title":"Cytotoxic and antimicrobial activities of phytoconstituents of the stem bark extract of Psychotria djumaensis","authors":"Mbasso Joseph Eric Tameko, N. R. Nono, M. Frese, J. R. Chouna, N. Lenta, Pépin Nkeng-Efouet-Alango, A. L. Tapondjou, N. Sewald","doi":"10.4314/cajeb.v14i2.3","DOIUrl":"https://doi.org/10.4314/cajeb.v14i2.3","url":null,"abstract":"Phytochemical investigation of the stem bark extract of Psychotria djumaensis led to the isolation of ten known pentacyclic triterpenoids among which one lupane-, three oleanane-, and six ursane-types. The structures of the isolated compounds were established by means of spectroscopic methods and by comparison with previously reported data. The isolated compounds were tested against five strains of bacteria, and the cytotoxicity against the human cervix carcinoma KB-3-1 cell was evaluated. The isolates exhibited weak antibacterial activity against the five strains. The cytotoxic activity exhibited by 3β,19α-dihydroxyurs-12-en-28-oic acid (5) (IC50 5.9 µM) was about three times more significant than that of the reference [(+) griseofulvin, IC50 17-21 µM].","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84660612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gueaba Helene Mbuntcha, Dongmo Hervet Paulin Fogang, A. J. Seukep, C. Schippa, E. Duñach, Verlaine Woguem, T. Fonkou, H. Womeni, A. L. Tapondjou
Background: The current study examined the chemical profile and in vitro antibacterial activity of essential oils (EOs) extracted from Aframomum danielli (leaves and seeds), Aframomum chlamydentum (leaves), and Aframomum melegueta (leaves) against foodborne pathogenic bacteria. Methods: The hydro-distillation technique using a Clevenger-type apparatus was used to extract EOs, whereas the Gas Chromatography-Mass Spectrometry (GC-MS) and GC coupled to Flame Ionization Detector (GC-FID) allowed the chemical characterization of oil constituents. The broth micro-dilution method was applied for the determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Besides, some modes of action were studied on the cell membrane integrity and biofilm formation of Salmonella typhi. Results: The major compounds identified from EOs of A. danielli seeds were eucalyptol (48.707%), limonene (11.368%), beta pinene (10.342-10.335%), and alpha terpineol (8.785-9.049%), whereas EOs from A. danielli leaves were dominated by sabinene (42.87%), beta pinene, (11.22%), caryophyllene (7.84%), terpinen-4-ol (5.68%), linalool (3.48%) and gamma terpinene (2.02%). Major volatile markers from EOs of A. chlamydentum leaves comprised beta pinene (49.72%), caryophyllene (10.62%), alpha pinene (6.21%) and linalool (2.96%), while those of EOs from A. melegueta included beta pinene (37.15%), caryophyllene (17.64%), caryophyllene oxide (8.72%) and alpha pinene (8.26%). This study is the first to report on the chemical constituents of EOs from A. chlamydentum. Test oils displayed significant antibacterial activity with the MIC ranging from 0.0625 to 0.5% (v/v). EOs from A. melegueta (leaves) appeared to be the most active, acting against all tested bacteria. All EOs identified displayed bactericidal effects against Citrobacter freundii, a bacterium known to cause a broad range of infections associated with a higher rate of in-hospital mortality. The EOs from A. melegueta may act through perturbation of cell membrane integrity and permeability as well as the inhibition of bacterial biofilm formation. Conclusion: Our findings suggest the possible application of essential oils in agricultural food products for the control of bacterial diseases.
{"title":"GC-MS/FID analysis, antibacterial activity and modes of action of essential oils from three Aframomum species found in Cameroon against foodborne pathogenic bacteria","authors":"Gueaba Helene Mbuntcha, Dongmo Hervet Paulin Fogang, A. J. Seukep, C. Schippa, E. Duñach, Verlaine Woguem, T. Fonkou, H. Womeni, A. L. Tapondjou","doi":"10.4314/cajeb.v14i2.5","DOIUrl":"https://doi.org/10.4314/cajeb.v14i2.5","url":null,"abstract":"Background: The current study examined the chemical profile and in vitro antibacterial activity of essential oils (EOs) extracted from Aframomum danielli (leaves and seeds), Aframomum chlamydentum (leaves), and Aframomum melegueta (leaves) against foodborne pathogenic bacteria. \u0000Methods: The hydro-distillation technique using a Clevenger-type apparatus was used to extract EOs, whereas the Gas Chromatography-Mass Spectrometry (GC-MS) and GC coupled to Flame Ionization Detector (GC-FID) allowed the chemical characterization of oil constituents. The broth micro-dilution method was applied for the determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Besides, some modes of action were studied on the cell membrane integrity and biofilm formation of Salmonella typhi. \u0000Results: The major compounds identified from EOs of A. danielli seeds were eucalyptol (48.707%), limonene (11.368%), beta pinene (10.342-10.335%), and alpha terpineol (8.785-9.049%), whereas EOs from A. danielli leaves were dominated by sabinene (42.87%), beta pinene, (11.22%), caryophyllene (7.84%), terpinen-4-ol (5.68%), linalool (3.48%) and gamma terpinene (2.02%). Major volatile markers from EOs of A. chlamydentum leaves comprised beta pinene (49.72%), caryophyllene (10.62%), alpha pinene (6.21%) and linalool (2.96%), while those of EOs from A. melegueta included beta pinene (37.15%), caryophyllene (17.64%), caryophyllene oxide (8.72%) and alpha pinene (8.26%). This study is the first to report on the chemical constituents of EOs from A. chlamydentum. Test oils displayed significant antibacterial activity with the MIC ranging from 0.0625 to 0.5% (v/v). EOs from A. melegueta (leaves) appeared to be the most active, acting against all tested bacteria. All EOs identified displayed bactericidal effects against Citrobacter freundii, a bacterium known to cause a broad range of infections associated with a higher rate of in-hospital mortality. The EOs from A. melegueta may act through perturbation of cell membrane integrity and permeability as well as the inhibition of bacterial biofilm formation. \u0000Conclusion: Our findings suggest the possible application of essential oils in agricultural food products for the control of bacterial diseases.","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78620014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Yakubu, Ya’u Anas, Halima Ibrahim, Fati Ahmed Abdullahi, A. S. Dalhatu
The scarcity and high price associated with fossil fuel has urged countries to research resources for alternative energy sources. Biofuels like bioethanol produced from lignocellulosic biomass (corn cob) were considered potential alternative. Cellulose composition from isolated cell wall material of corn cobs was investigated under two different pre-treatments using H2SO4 and NH4OH at varying concentrations of 5%, 10%, 20%, 30% and 40%. Cell wall not treated acted as control. Colorimetric anthrone-assay followed by absorbance reading at 625nm revealed that glucose is present in reasonable amount in corn cob. The analysis of variance (ANOVA) indicated significant differences among pre-treated compared to untreated (Control) corn cob samples at p≤0.05. Acid pre-treatment showed better glucose yield compared to alkali pre-treatment with results revealing 20% (19.37µg/ml) H2SO4 to be the optimal concentration producing highest glucose yield. The study reveals the potential of corn cob as a lignocellulosic feed stock for biofuel production.
{"title":"Effect of varying the concentration of sulphuric acid and ammonium hydroxide on the release of cellulose from isolated cell wall component of corn cob","authors":"S. Yakubu, Ya’u Anas, Halima Ibrahim, Fati Ahmed Abdullahi, A. S. Dalhatu","doi":"10.4314/cajeb.v14i2.1","DOIUrl":"https://doi.org/10.4314/cajeb.v14i2.1","url":null,"abstract":"The scarcity and high price associated with fossil fuel has urged countries to research resources for alternative energy sources. Biofuels like bioethanol produced from lignocellulosic biomass (corn cob) were considered potential alternative. Cellulose composition from isolated cell wall material of corn cobs was investigated under two different pre-treatments using H2SO4 and NH4OH at varying concentrations of 5%, 10%, 20%, 30% and 40%. Cell wall not treated acted as control. Colorimetric anthrone-assay followed by absorbance reading at 625nm revealed that glucose is present in reasonable amount in corn cob. The analysis of variance (ANOVA) indicated significant differences among pre-treated compared to untreated (Control) corn cob samples at p≤0.05. Acid pre-treatment showed better glucose yield compared to alkali pre-treatment with results revealing 20% (19.37µg/ml) H2SO4 to be the optimal concentration producing highest glucose yield. The study reveals the potential of corn cob as a lignocellulosic feed stock for biofuel production.","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91261075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Modjinan Kayangar, N. R. Nono, T. R. Fouedjou, T. B. Tchegnitegni, K. B. Ponou, B. R. Teponno, J. K. Siems, F. M. Melzig, L. Barboni, A. L. Tapondjou
Background: C. arborea belonging to Araliaceae family is used traditionally to cure many alien diseases including gonorrhoeae infection, diarrhea, malaria, and diabetes mellitus. The plant has been examined on the basis of scientific in vitro and in vivo evaluations possessing the major pharmacological activities including antimicrobial, antibacterial, antihyperglycemic, antiplasmodial and anticancer properties. Aim of the study: In the present paper, we reported the isolation and characterization of secondary metabolites from the methanol extract of the stem bark of Cussonia arborea Hochst after a short review of the traditional and pharmacological studies done on this important medicinal plant. Materials and methods: MeOH extract of stem bark of C. arborea was suspended in water and successively extracted with EtOAc and n-BuOH. The EtOAc extract (18 g) was subjected to repeated column chromatography to yield seven (1-7) compounds. Their structures were determined by means of NMR, and published data. Results: The isolated compounds were identified as: protocatechuic acid (1), mixture of 3,23-dihydroxyolean-12-en-28-oic acid (2a) and 3,23-dihydroxyurs-12-en-28-oic acid (2b) in ratio 5/4, 3-O-β-D-xylopyranosylolean-12-en-28-oic acid (3), 3-O-α-L-arabinopyranosylolean-12-en-28-oic acid (4), β-resorcylic acid (5), mixture of 3-O-β-D-glucopyranosyl-23-hydroxyolean-12-en-28-oic acid (6a) and 3-O-β-D-glucopyranosyl-23-hydroxyurs-12-en-28-oic acid (6b) in ration 4/1, 3-O-β-D-glucopyranosyl-(1→2)-α-L-arabinopyranosyl-3β-hydroxyolean-12-en-28-oic acid (7). Compounds 3, 4, 5, 7, 2b and 6b are herein reported for the first time in this plant
{"title":"Cussonia arborea Hochst (Araliaceae): Ethnobotany, Pharmacology and Phytochemistry","authors":"Modjinan Kayangar, N. R. Nono, T. R. Fouedjou, T. B. Tchegnitegni, K. B. Ponou, B. R. Teponno, J. K. Siems, F. M. Melzig, L. Barboni, A. L. Tapondjou","doi":"10.4314/cajeb.v14i2.2","DOIUrl":"https://doi.org/10.4314/cajeb.v14i2.2","url":null,"abstract":"Background: C. arborea belonging to Araliaceae family is used traditionally to cure many alien diseases including gonorrhoeae infection, diarrhea, malaria, and diabetes mellitus. The plant has been examined on the basis of scientific in vitro and in vivo evaluations possessing the major pharmacological activities including antimicrobial, antibacterial, antihyperglycemic, antiplasmodial and anticancer properties. \u0000Aim of the study: In the present paper, we reported the isolation and characterization of secondary metabolites from the methanol extract of the stem bark of Cussonia arborea Hochst after a short review of the traditional and pharmacological studies done on this important medicinal plant. \u0000Materials and methods: MeOH extract of stem bark of C. arborea was suspended in water and successively extracted with EtOAc and n-BuOH. The EtOAc extract (18 g) was subjected to repeated column chromatography to yield seven (1-7) compounds. Their structures were determined by means of NMR, and published data. \u0000Results: The isolated compounds were identified as: protocatechuic acid (1), mixture of 3,23-dihydroxyolean-12-en-28-oic acid (2a) and 3,23-dihydroxyurs-12-en-28-oic acid (2b) in ratio 5/4, 3-O-β-D-xylopyranosylolean-12-en-28-oic acid (3), 3-O-α-L-arabinopyranosylolean-12-en-28-oic acid (4), β-resorcylic acid (5), mixture of 3-O-β-D-glucopyranosyl-23-hydroxyolean-12-en-28-oic acid (6a) and 3-O-β-D-glucopyranosyl-23-hydroxyurs-12-en-28-oic acid (6b) in ration 4/1, 3-O-β-D-glucopyranosyl-(1→2)-α-L-arabinopyranosyl-3β-hydroxyolean-12-en-28-oic acid (7). Compounds 3, 4, 5, 7, 2b and 6b are herein reported for the first time in this plant","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82611459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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