Journal of biophotonics最新文献

Photobiomodulation shows promise in modulating complex mechanisms of chronic wound healing. Current photobiomodulation devices remain costly and technically demanding. Therefore, this study reports the development of a simple, portable and operationally and easy-to-use LED-based photobiomodulation device for the treatment of skin lesions. The design and operating parameters confirmed the device's safety and energy efficiency. In vitro LED irradiation on fibroblasts increased cell viability, without inducing proliferation. The treatment prevented cellular shrinkage, as evidenced by a higher number of smaller cells in the control group. Irradiation also enhanced cell migration at 72 and 96 h after treatment. Significantly increased adhesion to fibronectin was observed in the irradiated group, with no effect on type I collagen adhesion. Nevertheless, no significant differences were observed in the proportion of cells in the sub-G0/G1, G0/G1, S, and G2/M phases of the cell cycle. In conclusion, the LED device provides a reliable source of electromagnetic stimulation with healing effects.

Accurate identification of dysplasia in Barrett's esophagus (BE) remains a challenge. Advanced optical imaging techniques may allow for better localization of dysplasia in BE. Here, we have assessed the potential clinical utility of a previously described multimodal imaging probe combining optical coherence tomography (OCT) with angle-resolved low coherence interferometry (a/LCI) to prospectively identify dysplasia in BE. Imaging was conducted on 37 patients undergoing endoscopic surveillance of BE, yielding co-registered biopsies of 50 esophageal sites. The a/LCI nuclear morphology data were compared to a previous decision line to prospectively predict dysplasia, demonstrating 100% sensitivity, 93% specificity, and 94% overall accuracy. The NPV was 100%, comparable to previous a/LCI studies. The addition of OCT imaging markedly improved PPV and specificity, compared to previous studies with a/LCI alone, illustrating the clinical utility of the combined platform. These findings suggest that combining OCT and a/LCI enables better detection of dysplasia by providing better guidance.

Analysis of sequential alterations in body fluid constituents, such as serum, is vital for assessing inflammation. Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy was used to monitor biomolecular changes in a Dextran Sodium Sulfate (DSS) mouse colitis model. Serum samples collected on days 0, 3, and 7 post- (3% DSS) administration showed stepwise changes in lipid, protein, and carbohydrate regions. Statistically significant (SS) changes (p < 0.05) were detected at the initial stage of colitis (day 0 to 3) in six spectral biomarkers (SBMs), including the integral ratio of α-helix to β-sheet (Amide I) and the β-sheet (Amide I) to Tyrosine (Amide II). Changes intensified from day 3 to 7, with the most prominent differences from day 0 to 7, even when the initial changes (day 0 to 3) were not SS. These results highlight the potential of ATR-FTIR as a minimally invasive pre-screening tool for monitoring colitis progression.

Microbiological water quality assessment relies on culture-based methods that are time-consuming, resource-intensive, and often lack specificity. To address these limitations, we developed a prototype for automated, label-free, and nondestructive microbial identification based on discrete frequency infrared (DFIR) multispectral imaging. By combining monochromatic quantum cascade lasers (QCLs) with an uncooled bolometer array, this prototype captures spectral and morphological fingerprints of colonies directly on filtration membranes. A demonstration database of 3230 colonies from 11 strains across 7 genera was acquired. In average, deep-learning based classification achieved a 96.5% ± 1.3% correct identification rate. Overall, this prototype brings DFIR imaging one step closer to an industry-ready microbial identification tool.

A comprehensive image catalog from an in vitro investigation of teeth with occlusal carious lesions is provided. The aim was to visualize carious lesions using various imaging techniques in order to present different stages of caries progression and to provide reference data for the development and validation of polarization-sensitive optical coherence tomography (PS-OCT) as a non-ionizing diagnostic method for dental caries. The study covers a variety of approaches, bridging the gap from basic histopathological analysis of thin sections to future optical imaging of occlusal carious lesions in vivo. All measurements were performed to support the interpretation and validation of PS-OCT imaging based on the degree of polarization (DOP).

Currently, local excision of choroidal melanoma primarily depends on the intraoperative visual assessment. To improve surgical precision, we employed multiphoton microscopy (MPM) to characterize the microstructural and spectral features of both normal tissue and tumor boundary. The spectral differences between normal choroid and tumor boundary were systematically analyzed using lambda mode MPM. Collagen parameters were quantified by combining with image processing techniques to elucidate the structural alterations of normal tissues and choroidal melanomas. The results of the study demonstrated that the MPM enables label-free identification of tumor boundary, with NADH molecular changes serving as a critical basis for qualitative differentiation. Furthermore, utilizing self-developed image processing algorithms, the alteration of collagen fiber provides a potential quantitative index for identifying the tumor boundary. This study confirms the capability of MPM in identifying tumor boundary. With the advancement of multiphoton endoscopes, it holds promise for providing precise intraoperative guidance during choroidal melanoma resection.